##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146454_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3606452
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.797544511891466	31.0	31.0	34.0	30.0	34.0
2	31.73796933939506	31.0	31.0	34.0	30.0	34.0
3	31.937157904777326	31.0	31.0	34.0	30.0	34.0
4	35.694115712617275	37.0	35.0	37.0	33.0	37.0
5	35.605874416185216	37.0	35.0	37.0	33.0	37.0
6	35.64706503788211	37.0	35.0	37.0	33.0	37.0
7	35.55885368777957	37.0	35.0	37.0	33.0	37.0
8	35.539852464416555	37.0	35.0	37.0	33.0	37.0
9	37.11765829685242	39.0	37.0	39.0	33.0	39.0
10-11	37.066739831834724	39.0	37.0	39.0	33.0	39.0
12-13	37.0157175251466	39.0	37.0	39.0	33.0	39.0
14-15	38.22092641188625	40.0	37.0	41.0	33.0	41.0
16-17	38.21586298666945	40.0	37.0	41.0	33.0	41.0
18-19	38.26231251656753	40.0	37.5	41.0	33.0	41.0
20-21	38.2739110350006	40.0	38.0	41.0	33.0	41.0
22-23	38.24597402100458	40.0	37.5	41.0	33.0	41.0
24-25	38.18663134848322	40.0	37.0	41.0	33.0	41.0
26-27	38.04954259754462	40.0	37.0	41.0	33.0	41.0
28-29	37.975743195805734	40.0	37.0	41.0	33.0	41.0
30-31	37.847298813348964	40.0	37.0	41.0	33.0	41.0
32-33	37.73306770754193	40.0	36.5	41.0	32.0	41.0
34-35	37.63968659502469	40.0	36.0	41.0	32.0	41.0
36-37	37.555603401903035	40.0	36.0	41.0	32.0	41.0
38-39	37.446311638141864	40.0	36.0	41.0	31.5	41.0
40-41	37.31792479145709	40.0	36.0	41.0	31.0	41.0
42-43	37.196922210527134	39.0	35.5	41.0	31.0	41.0
44-45	37.025884997221645	39.0	35.0	41.0	31.0	41.0
46-47	36.80858209120765	39.0	35.0	41.0	30.0	41.0
48-49	36.579629231166805	39.0	35.0	41.0	30.0	41.0
50-51	36.386923630204976	39.0	35.0	41.0	30.0	41.0
52-53	36.20610325605332	38.5	35.0	40.5	29.0	41.0
54-55	36.00838996332129	38.0	35.0	40.0	29.0	41.0
56-57	35.76899235037649	38.0	34.0	40.0	28.5	41.0
58-59	35.50962081292084	37.0	34.0	40.0	28.0	41.0
60-61	35.2501439087502	37.0	34.0	40.0	28.0	41.0
62-63	34.97446770399273	36.0	34.0	39.5	28.0	41.0
64-65	34.70593966036426	36.0	33.5	39.0	28.0	41.0
66-67	34.47139418464463	35.0	33.0	39.0	27.5	41.0
68-69	34.26681028889335	35.0	33.0	39.0	27.0	41.0
70-71	34.02897751030653	35.0	33.0	38.0	27.0	40.0
72-73	33.80839520392895	35.0	33.0	37.0	26.5	40.0
74-75	33.61703996060394	35.0	33.0	37.0	27.0	39.0
76-77	33.44083589633246	35.0	33.0	36.0	27.0	39.0
78-79	33.31618748842352	35.0	33.0	36.0	27.0	39.0
80-81	33.151964590129026	35.0	33.0	36.0	27.0	37.5
82-83	32.95909470027606	35.0	33.0	35.0	27.0	37.0
84-85	32.81389451460882	35.0	33.0	35.0	27.0	37.0
86-87	32.672436649649015	35.0	33.0	35.0	27.0	36.0
88-89	32.555949587017935	35.0	33.0	35.0	26.5	36.0
90-91	32.47080357647904	35.0	33.0	35.0	26.0	36.0
92-93	32.39417965357642	35.0	33.0	35.0	26.0	36.0
94-95	32.34666619713779	35.0	33.0	35.0	26.0	35.0
96-97	32.29526415435447	35.0	33.0	35.0	26.0	35.0
98-99	32.252601587377285	35.0	33.0	35.0	26.0	35.0
100	31.753018201822734	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5138518430874441
1101	2	0.6264954764983699
1101	3	0.4680277099327661
1101	4	0.35146187246736815
1101	5	0.3794789712083855
1101	6	0.4170338503656126
1101	7	0.4113250710507472
1101	8	0.44658201340158143
1101	9	0.5538477464369009
1101	10-11	0.5614178028808183
1101	12-13	0.5840507237293338
1101	14-15	0.7973468781784305
1101	16-17	0.7419495230305984
1101	18-19	0.7648721572954855
1101	20-21	0.737984095058529
1101	22-23	0.7618629879244025
1101	24-25	0.7532227409277326
1101	26-27	0.7710260175162773
1101	28-29	0.8320514992374015
1101	30-31	0.8087463919583726
1101	32-33	0.8118055528042092
1101	34-35	0.7425372111359962
1101	36-37	0.8353584542056893
1101	38-39	0.8956174571282105
1101	40-41	0.8296907482013722
1101	42-43	0.8604655170669773
1101	44-45	0.8303415533749572
1101	46-47	0.8481837215592378
1101	48-49	0.8868037054058746
1101	50-51	0.8747250375261402
1101	52-53	0.943752230110789
1101	54-55	0.8695032175913369
1101	56-57	0.8997748268306296
1101	58-59	0.8242407576195987
1101	60-61	0.8135238859763305
1101	62-63	0.8262748495847774
1101	64-65	0.8300502171777282
1101	66-67	0.7613420326568203
1101	68-69	0.6991456742040825
1101	70-71	0.7282267348642009
1101	72-73	0.7239072594751619
1101	74-75	0.6890608353201984
1101	76-77	0.7080035461883654
1101	78-79	0.7248167735744957
1101	80-81	0.7036909412063821
1101	82-83	0.6517051353617163
1101	84-85	0.6663227669997056
1101	86-87	0.6995200194146278
1101	88-89	0.6944702687353939
1101	90-91	0.6485487819654878
1101	92-93	0.6788407689548706
1101	94-95	0.7061367300994092
1101	96-97	0.716586200460732
1101	98-99	0.6549554837647023
1101	100	0.7609940700912432
1102	1	0.4225816818435675
1102	2	0.5375013289514357
1102	3	0.4211878886779381
1102	4	0.3203849704201218
1102	5	0.34124704020605634
1102	6	0.3705060421902999
1102	7	0.3459317218518265
1102	8	0.372421350087734
1102	9	0.4879093918662534
1102	10-11	0.49491175037999113
1102	12-13	0.437534319428579
1102	14-15	0.5889265951012419
1102	16-17	0.6523480135722934
1102	18-19	0.5983844201083386
1102	20-21	0.5910660593509647
1102	22-23	0.5639054717727561
1102	24-25	0.6287607388681167
1102	26-27	0.638305268070539
1102	28-29	0.6562969428711654
1102	30-31	0.6833759580198304
1102	32-33	0.7195536216329259
1102	34-35	0.7222162746206351
1102	36-37	0.6904472316412296
1102	38-39	0.6642284268107943
1102	40-41	0.6578074424754021
1102	42-43	0.7493003943390164
1102	44-45	0.7719612798509132
1102	46-47	0.6779985180288719
1102	48-49	0.689262910636451
1102	50-51	0.639303912021326
1102	52-53	0.6139079448342244
1102	54-55	0.6749799955398501
1102	56-57	0.6881050154591506
1102	58-59	0.6211515363160842
1102	60-61	0.6476117564774668
1102	62-63	0.5938347429643116
1102	64-65	0.6241453523693252
1102	66-67	0.6127252420066114
1102	68-69	0.5867991561487145
1102	70-71	0.5591593414213136
1102	72-73	0.5528528656813378
1102	74-75	0.49428611367841313
1102	76-77	0.42832878315473977
1102	78-79	0.46191442222611556
1102	80-81	0.49138520064184377
1102	82-83	0.5135777974714344
1102	84-85	0.4949624268340287
1102	86-87	0.518170230712677
1102	88-89	0.5149130385637335
1102	90-91	0.5081257711736598
1102	92-93	0.5605058344714706
1102	94-95	0.5433046450487709
1102	96-97	0.5209249715607811
1102	98-99	0.5606805359652682
1102	100	0.5991397616920793
1103	1	0.23221855195438934
1103	2	-0.27061786904431173
1103	3	0.15452829078238395
1103	4	0.19215002437422157
1103	5	0.20994068367917862
1103	6	0.2076900899270342
1103	7	0.26403192834448674
1103	8	0.27426645988136755
1103	9	0.3339293499876632
1103	10-11	0.39758404284668103
1103	12-13	0.3885944371062209
1103	14-15	0.48907209620300307
1103	16-17	0.48325441999260477
1103	18-19	0.4316073875035329
1103	20-21	0.4834444873236876
1103	22-23	0.45669787234615455
1103	24-25	0.4640829199173808
1103	26-27	0.4617407165182428
1103	28-29	0.5610274731290659
1103	30-31	0.5703702271781879
1103	32-33	0.5607236991020628
1103	34-35	0.561272583509556
1103	36-37	0.5569153626939922
1103	38-39	0.5266240012356747
1103	40-41	0.44930926256356685
1103	42-43	0.5386012233116233
1103	44-45	0.533871329765212
1103	46-47	0.5657988732439918
1103	48-49	0.5916275965114437
1103	50-51	0.5813684565904609
1103	52-53	0.5250141614946813
1103	54-55	0.4828656458328098
1103	56-57	0.4990603906442175
1103	58-59	0.4966148659020533
1103	60-61	0.5807896223303786
1103	62-63	0.5155645707864878
1103	64-65	0.5498245416770544
1103	66-67	0.5843364605584114
1103	68-69	0.5124158582689375
1103	70-71	0.48411671073827733
1103	72-73	0.43635281546148263
1103	74-75	0.4472651405114334
1103	76-77	0.4210187855418539
1103	78-79	0.36852403625783126
1103	80-81	0.3577992590737651
1103	82-83	0.41967687873988524
1103	84-85	0.4111214904514142
1103	86-87	0.424292428628533
1103	88-89	0.4153417052185233
1103	90-91	0.4436603444475722
1103	92-93	0.40047954517123685
1103	94-95	0.42823156620939073
1103	96-97	0.3963636460650548
1103	98-99	0.3884896326977909
1103	100	0.41213258573891665
1104	1	0.3712763805626196
1104	2	0.4251598595794661
1104	3	0.39665999600772395
1104	4	0.3007049735079761
1104	5	0.25339266996738274
1104	6	0.27239099971907166
1104	7	0.24873900133850668
1104	8	0.3041112195463711
1104	9	0.2633308110565551
1104	10-11	0.30492052379849355
1104	12-13	0.3160234942840745
1104	14-15	0.4457118979506234
1104	16-17	0.4746610418332793
1104	18-19	0.47777552015328695
1104	20-21	0.4402975377067051
1104	22-23	0.4398845421865758
1104	24-25	0.3830541692808822
1104	26-27	0.5176564425261603
1104	28-29	0.5083620403702653
1104	30-31	0.5093310475521946
1104	32-33	0.4462908391644902
1104	34-35	0.4798511501028315
1104	36-37	0.4612340149028711
1104	38-39	0.5180108096802485
1104	40-41	0.5196678136016288
1104	42-43	0.4812922176600125
1104	44-45	0.4645978575315084
1104	46-47	0.43903419087921236
1104	48-49	0.5434856747983474
1104	50-51	0.49024670222572553
1104	52-53	0.4816576977754963
1104	54-55	0.4867141977193583
1104	56-57	0.4883444260390135
1104	58-59	0.5233641185336921
1104	60-61	0.5013801245460598
1104	62-63	0.5655660727478349
1104	64-65	0.4079589823732732
1104	66-67	0.43072645978813995
1104	68-69	0.46009836178265573
1104	70-71	0.49537624683483017
1104	72-73	0.4775185884294615
1104	74-75	0.45129197222256323
1104	76-77	0.43258530026688646
1104	78-79	0.36916227477060204
1104	80-81	0.3991030468084418
1104	82-83	0.4155492689530291
1104	84-85	0.4274641336274456
1104	86-87	0.34409915417787573
1104	88-89	0.3502434682549733
1104	90-91	0.3883025169583405
1104	92-93	0.4228421472136219
1104	94-95	0.37550316243090265
1104	96-97	0.3640894707070217
1104	98-99	0.37268364873167314
1104	100	0.4439049348966364
1105	1	0.1801611346640044
1105	2	-0.39733856191721983
1105	3	0.060126002009759816
1105	4	0.1268032887247017
1105	5	0.1563267173858094
1105	6	0.2079421090835396
1105	7	0.24220160275727665
1105	8	0.24404988310935494
1105	9	0.30742960351904713
1105	10-11	0.32923395171869174
1105	12-13	0.30696858113924463
1105	14-15	0.40204535325635504
1105	16-17	0.42726981724373303
1105	18-19	0.45076805121390606
1105	20-21	0.4358813692587944
1105	22-23	0.41673574927639834
1105	24-25	0.412670079213477
1105	26-27	0.42128851851180116
1105	28-29	0.47891337951174506
1105	30-31	0.44302639743731476
1105	32-33	0.4227758863448301
1105	34-35	0.4717273732204035
1105	36-37	0.4646814251487825
1105	38-39	0.4452710953567163
1105	40-41	0.5020165704378954
1105	42-43	0.5390543546595339
1105	44-45	0.4747490659050513
1105	46-47	0.4799915513864974
1105	48-49	0.5523186994070315
1105	50-51	0.5587468824334678
1105	52-53	0.5175261313446455
1105	54-55	0.4655987155711401
1105	56-57	0.5678104307465262
1105	58-59	0.5083471123382353
1105	60-61	0.48289773317799956
1105	62-63	0.4983273197659983
1105	64-65	0.5318028965781991
1105	66-67	0.49756418177242523
1105	68-69	0.43403466414416414
1105	70-71	0.45675643587119197
1105	72-73	0.46519323648498556
1105	74-75	0.45475772147845106
1105	76-77	0.3841782967931522
1105	78-79	0.35837071131929576
1105	80-81	0.38568122433589025
1105	82-83	0.35748455177346017
1105	84-85	0.3763934428929474
1105	86-87	0.38907406587284754
1105	88-89	0.3963233322701427
1105	90-91	0.3585145304769952
1105	92-93	0.41347005010359794
1105	94-95	0.42361264243690044
1105	96-97	0.36476738030152944
1105	98-99	0.39970350998735427
1105	100	0.4458880021650984
1106	1	0.2405932406847242
1106	2	0.3236181930532709
1106	3	0.2801899734538402
1106	4	0.22015849700412815
1106	5	0.23031813596293915
1106	6	0.21326186104809608
1106	7	0.221541493896396
1106	8	0.22574712185333823
1106	9	0.3079704925714708
1106	10-11	0.29385323468066105
1106	12-13	0.2540130677493835
1106	14-15	0.2865252473031674
1106	16-17	0.2733907741028645
1106	18-19	0.3443530023444836
1106	20-21	0.3705487530541305
1106	22-23	0.3135241242486586
1106	24-25	0.2522693114815908
1106	26-27	0.38525010308181606
1106	28-29	0.33291758941737726
1106	30-31	0.3239080211011114
1106	32-33	0.3700412732171401
1106	34-35	0.307066799260177
1106	36-37	0.3394734488813498
1106	38-39	0.24254324437006858
1106	40-41	0.3170356529970917
1106	42-43	0.2855524865872354
1106	44-45	0.3258276424773783
1106	46-47	0.3988204768381536
1106	48-49	0.2669854434440708
1106	50-51	0.3232688064624156
1106	52-53	0.3282378369589054
1106	54-55	0.34443668973784725
1106	56-57	0.3556511061514769
1106	58-59	0.2857416777587005
1106	60-61	0.27330478775491684
1106	62-63	0.3237619771450042
1106	64-65	0.3351563949980516
1106	66-67	0.27616405018066104
1106	68-69	0.3095007913818435
1106	70-71	0.2929237650650478
1106	72-73	0.27714402838186203
1106	74-75	0.1939083362604066
1106	76-77	0.1984855865730708
1106	78-79	0.19064616383180066
1106	80-81	0.3057688207875344
1106	82-83	0.21811278181278482
1106	84-85	0.2209843385677246
1106	86-87	0.3084434144004504
1106	88-89	0.21627848998289778
1106	90-91	0.2294738017204878
1106	92-93	0.27975474123026345
1106	94-95	0.23509466199431728
1106	96-97	0.26741103756123863
1106	98-99	0.26550203688768903
1106	100	0.2212159070734394
1107	1	0.06906467250808035
1107	2	-0.5027606395300381
1107	3	-0.06907349640204785
1107	4	0.05895645354338086
1107	5	0.06116638229889304
1107	6	0.15392248371703232
1107	7	0.19078309767163404
1107	8	0.18031349930698326
1107	9	0.18498692168071784
1107	10-11	0.17290841862715212
1107	12-13	0.09859240288044901
1107	14-15	0.1857016577638646
1107	16-17	0.23529646103487067
1107	18-19	0.2154527852158381
1107	20-21	0.1876074697187491
1107	22-23	0.14196305243861218
1107	24-25	0.15565477830818253
1107	26-27	0.2081579963210629
1107	28-29	0.14431952475155896
1107	30-31	0.2423697018344484
1107	32-33	0.2982863834449532
1107	34-35	0.28360793113080973
1107	36-37	0.2578217499372215
1107	38-39	0.29793288304348664
1107	40-41	0.22671938279449222
1107	42-43	0.33028919621268926
1107	44-45	0.2855645053186109
1107	46-47	0.3648562592892546
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2216	86-87	-0.8443713912395374
2216	88-89	-0.8944001799599306
2216	90-91	-1.0318741314887703
2216	92-93	-1.068752785784639
2216	94-95	-1.0015400325732386
2216	96-97	-1.1704285317401855
2216	98-99	-1.0500846752613207
2216	100	-1.0099196896646845
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	10.0
13	44.0
14	203.0
15	597.0
16	1505.0
17	2987.0
18	5175.0
19	7957.0
20	11582.0
21	16475.0
22	22434.0
23	26685.0
24	29323.0
25	32055.0
26	36817.0
27	45367.0
28	57321.0
29	70746.0
30	88480.0
31	108652.0
32	135738.0
33	170392.0
34	228023.0
35	311000.0
36	467042.0
37	776991.0
38	711538.0
39	240857.0
40	452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.788932908921794	20.67805456228539	13.704188706726192	24.828823822066624
2	15.173854752011401	20.037767083574227	39.61315082015852	25.17522734425584
3	18.2616587436973	25.63570460449114	29.67145557110819	26.431181080703375
4	12.592916581496311	16.803572523123577	37.62701014749887	32.97650074788123
5	13.002589043606683	38.26416230699674	34.86119348893105	13.872055160465525
6	30.98604944693566	37.87999951198574	17.30201871534683	13.831932325731772
7	26.969297683753336	32.0508213367317	22.984091227965102	17.995789751549868
8	26.491327210233216	35.011390696451805	19.376772517698836	19.120509575616147
9	26.242412210116754	14.623069986790341	20.712018349336134	38.422499453756764
10-11	25.74892165485635	25.71415063891049	27.35838436224855	21.178543343984614
12-13	26.112104084568433	23.37227835002379	27.815842273791525	22.69977529161625
14-15	23.350653438219915	24.558448248011885	25.368765715482095	26.72213259828611
16-17	22.245492971474263	27.214187682551795	26.579042267096593	23.961277078877348
18-19	21.894482444241596	26.859015453415157	28.916827397120493	22.32967470522275
20-21	23.131039785517643	26.03781751614226	28.41659687274233	22.414545825597774
22-23	23.36892445258794	25.683377772552536	28.190473444054277	22.75722433080525
24-25	22.886416190540757	25.848399735862245	28.75036583744437	22.514818236152628
26-27	22.872644476476616	25.989759717572174	27.986893458888236	23.15070234706297
28-29	22.291285174459553	26.46323866226419	28.408225036684254	22.837251126592008
30-31	22.963982329447337	26.12765953907053	28.20115171365098	22.707206417831156
32-33	23.2195104773334	26.2947628306158	27.607867788064283	22.87785890398652
34-35	23.23554334528604	26.206121500561775	28.218468316088234	22.33986683806395
36-37	23.11627882472857	25.77262916572853	27.96101265176966	23.150079357773233
38-39	23.14578150492506	25.840493648605335	28.425652136781526	22.58807270968808
40-41	22.839508911426982	25.36076465497664	28.829922718738743	22.96980371485763
42-43	22.955651215116582	25.496963958891683	28.074281902832542	23.473102923159193
44-45	23.044504682164078	26.165452916051567	27.903199598941008	22.88684280284335
46-47	23.259466644779966	25.99177806886103	27.309264063406363	23.43949122295264
48-49	23.796626223663907	25.930548837237676	27.171178950062437	23.101645989035983
50-51	23.30574481512578	26.023415811440167	27.04441927966877	23.626420093765287
52-53	22.983156298766765	26.379957365299745	26.893426004283434	23.743460331650056
54-55	22.90758279843358	26.37977517434788	26.85112655447659	23.86151547274195
56-57	23.653135354711697	25.971287554243094	26.65863938221797	23.716937708827242
58-59	22.849275200772396	26.135556685014365	27.366699136296745	23.648468977916494
60-61	22.890501739938166	26.6369560093721	26.864520511860697	23.608021738829045
62-63	23.207864682519	26.094053102606107	26.577325859321014	24.12075635555388
64-65	22.56308970700289	26.2038840389391	26.877135755584714	24.355890498473293
66-67	23.062403159670502	25.95782503136046	26.841227888240297	24.13854392072874
68-69	22.76556075218563	25.849594484467463	27.127074473498062	24.257770289848843
70-71	22.64460454156502	25.65114398703202	27.208976098748288	24.49527537265467
72-73	23.072357541428527	25.74297398107614	27.350925507950752	23.833742969544584
74-75	23.16217960840118	26.130059898304314	26.963082214457113	23.74467827883739
76-77	23.030575194203205	25.99694953759887	26.60930192770313	24.363173340494797
78-79	22.788208064981696	26.107306641940465	26.7456662833699	24.358819009707936
80-81	22.54559697198672	26.471518789903847	27.07298981650295	23.909894421606484
82-83	23.158696970188522	25.725162867634978	27.778385497144715	23.337754665031788
84-85	23.336640576602797	26.10153939326043	26.66908563134978	23.892734398786985
86-87	22.367665397628436	26.59560366851786	26.989736679882082	24.046994253971626
88-89	22.969634080464807	25.760395857297034	26.832980787255543	24.43698927498261
90-91	22.626418838408334	25.823198723756015	26.99674477920985	24.553637658625806
92-93	22.910354728443902	26.06052001104241	26.778138078112995	24.250987182400692
94-95	22.022918247197193	25.94397878872577	27.275589621071965	24.757513343005073
96-97	22.32087311423583	25.86958773256585	26.942970196160097	24.866568957038222
98-99	21.806874055144615	26.387599504432707	26.834281100230374	24.971245340192304
100	22.390325803407894	25.827884775489817	26.31076205805628	25.471027363046012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	128.0
2	132.0
3	181.0
4	139.0
5	111.0
6	234.5
7	547.5
8	920.0
9	1052.0
10	890.5
11	657.0
12	618.0
13	740.5
14	862.5
15	1031.0
16	1330.5
17	1687.0
18	2090.5
19	2627.0
20	3260.5
21	3962.5
22	4786.5
23	5634.0
24	6498.5
25	7693.0
26	9574.0
27	11914.0
28	15084.0
29	21045.0
30	29529.0
31	37302.5
32	48434.0
33	58316.5
34	64313.0
35	72526.5
36	81237.5
37	96121.5
38	103454.5
39	98569.5
40	94748.0
41	91569.5
42	91621.5
43	97437.5
44	107032.5
45	125325.0
46	143195.5
47	154741.0
48	162346.0
49	159851.0
50	153986.5
51	156729.5
52	158816.5
53	155561.5
54	152231.5
55	137017.5
56	122146.5
57	105351.5
58	87880.5
59	82918.5
60	70359.5
61	52480.0
62	40648.5
63	32193.5
64	23267.0
65	14296.0
66	9695.5
67	7225.0
68	5452.0
69	3981.0
70	2870.0
71	2171.5
72	1652.5
73	1361.5
74	1086.0
75	789.5
76	529.5
77	296.0
78	154.5
79	59.0
80	25.0
81	12.0
82	9.0
83	8.0
84	3.0
85	2.0
86	1.5
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014363146937765981
2	0.003798747356127296
3	0.001663684973486407
4	0.02587030133771363
5	0.002800536372035452
6	0.0
7	3.8819316048016166E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009275043727186719
16-17	0.0031194093252870135
18-19	0.0
20-21	4.1592124337160174E-5
22-23	7.763863209603233E-4
24-25	1.3864041445720059E-5
26-27	2.35688704577241E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.4364932626304187E-4
36-37	0.0
38-39	0.0
40-41	4.1592124337160177E-4
42-43	7.902503624060434E-4
44-45	0.0
46-47	0.0
48-49	5.129695334916422E-4
50-51	0.0
52-53	0.0
54-55	3.6046507758872155E-4
56-57	5.5456165782880234E-5
58-59	1.386404144572006E-4
60-61	5.5456165782880234E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015250445590292065
70-71	8.318424867432035E-4
72-73	0.0
74-75	1.663684973486407E-4
76-77	0.0031471374081784536
78-79	5.822897407202425E-4
80-81	0.003521466527212895
82-83	0.002010286009629409
84-85	0.006266546733465467
86-87	0.008567977613454998
88-89	0.008429337198997796
90-91	0.006793380308402829
92-93	0.009857333467906963
94-95	0.001885509636617928
96-97	0.0052267436250364625
98-99	0.0022182466313152094
100	0.08462610898467524
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3606452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.846892958068594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.94604277205947	21.63870351086128
2	15.77023250538637	10.044658130457872
3	6.147104697542113	5.872985559139928
4	3.098691062301763	3.947347302849924
5	1.726666348475393	2.7494479187097505
6	1.0861594527803715	2.0754482296854513
7	0.7417794517789029	1.6536359559508267
8	0.5174826922642124	1.3184172726553216
9	0.38560147556666075	1.1052188025160272
>10	2.1165368189855185	12.437738852254816
>50	0.20015743776737627	4.461092871307571
>100	0.2105633914164465	14.298784237856676
>500	0.03642505190191404	7.928241738141614
>1k	0.01577257032110852	8.676124666813038
>5k	6.971301799384984E-4	1.488744655078592
>10k+	8.71412724923123E-5	0.3034102957213952
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	10933	0.3031511302521148	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7814	0.2166672397137131	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7750	0.21489264240866093	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	6878	0.19071375412732514	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6857	0.1901314643866049	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6787	0.18819049858420409	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6293	0.17449282563583268	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5649	0.15663594025374525	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5617	0.15574864160121915	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4244	0.11767798379127185	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	4065	0.11271465695370408	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	3953	0.1096091116698628	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	3864	0.10714131229252462	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	3831	0.1062262855571071	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1091233156576048E-4
2	0.0	0.0	0.0	0.0	1.1091233156576048E-4
3	0.0	0.0	0.0	0.0	1.1091233156576048E-4
4	0.0	0.0	0.0	0.0	1.1091233156576048E-4
5	0.0	0.0	0.0	0.0	1.386404144572006E-4
6	0.0	0.0	0.0	0.0	1.386404144572006E-4
7	0.0	0.0	0.0	0.0	1.386404144572006E-4
8	0.0	0.0	0.0	0.0	1.386404144572006E-4
9	0.0	0.0	0.0	2.772808289144012E-5	1.386404144572006E-4
10-11	0.0	0.0	0.0	2.772808289144012E-5	1.386404144572006E-4
12-13	0.0	0.0	0.0	2.772808289144012E-5	2.772808289144012E-4
14-15	0.0	1.386404144572006E-5	0.0	5.545616578288024E-5	3.743291190344416E-4
16-17	0.0	2.772808289144012E-5	0.0	5.545616578288024E-5	4.7137740915448203E-4
18-19	0.0	2.772808289144012E-5	0.0	5.545616578288024E-5	5.268335749373622E-4
20-21	0.0	2.772808289144012E-5	0.0	6.93202072286003E-5	5.961537821659625E-4
22-23	0.0	2.772808289144012E-5	0.0	1.2477637301148054E-4	6.654739893945629E-4
24-25	0.0	2.772808289144012E-5	0.0	3.6046507758872155E-4	6.654739893945629E-4
26-27	0.0	2.772808289144012E-5	0.0	7.625222795146033E-4	6.93202072286003E-4
28-29	0.0	2.772808289144012E-5	0.0	0.002467799377338171	7.347941966231632E-4
30-31	0.0	2.772808289144012E-5	0.0	0.007583630670808873	7.902503624060434E-4
32-33	0.0	2.772808289144012E-5	0.0	0.02068514983701433	8.595705696346436E-4
34-35	0.0	4.159212433716018E-5	0.0	0.04137029967402865	9.704829012004042E-4
36-37	0.0	5.545616578288024E-5	0.0	0.07593335499820877	9.704829012004042E-4
38-39	0.0	5.545616578288024E-5	0.0	0.13295615746445538	9.704829012004042E-4
40-41	0.0	5.545616578288024E-5	0.0	0.19790919163765386	9.704829012004042E-4
42-43	0.0	5.545616578288024E-5	0.0	0.26530229710529907	9.704829012004042E-4
44-45	0.0	5.545616578288024E-5	0.0	0.3473635584225161	9.982109840918443E-4
46-47	0.0	5.545616578288024E-5	0.0	0.45274413745143427	0.0010259390669832844
48-49	0.0	5.545616578288024E-5	0.0	0.5754270402046111	0.0010813952327661646
50-51	0.0	5.545616578288024E-5	0.0	0.7105182600517073	0.0011368513985490448
52-53	0.0	5.545616578288024E-5	0.0	0.8416859561696648	0.0012477637301148055
54-55	0.0	1.1091233156576048E-4	0.0	0.9875911283444228	0.0012616277715605255
56-57	0.0	1.6636849734864072E-4	0.0	1.1686693736669724	0.0013032198958976857
58-59	0.0	1.6636849734864072E-4	0.0	1.3902999402182532	0.0013309479787891258
60-61	0.0	1.6636849734864072E-4	0.0	1.6210391819993721	0.0013309479787891258
62-63	0.0	1.6636849734864072E-4	0.0	1.861843717870084	0.0013586760616805659
64-65	0.0	1.6636849734864072E-4	0.0	2.1141970002650803	0.001414132227463446
66-67	0.0	1.9409658024008083E-4	0.0	2.403317720574127	0.001414132227463446
68-69	0.0	1.9409658024008083E-4	0.0	2.73378101247431	0.001414132227463446
70-71	0.0	1.9409658024008083E-4	0.0	3.10134447928324	0.0014418603103548862
72-73	0.0	1.9409658024008083E-4	0.0	3.4875689458781096	0.0015666366833663667
74-75	0.0	1.9409658024008083E-4	0.0	3.8710344682252806	0.001663684973486407
76-77	0.0	1.9409658024008083E-4	0.0	4.299655173561162	0.0017191411392692873
78-79	0.0	1.9409658024008083E-4	0.0	4.776883208205738	0.0017468692221607276
80-81	0.0	1.9409658024008083E-4	0.0	5.296368841176868	0.0017884613464978878
82-83	0.0	1.9409658024008083E-4	0.0	5.836109838700196	0.0019271017609550883
84-85	0.0	1.9409658024008083E-4	0.0	6.370083394982104	0.0019409658024008083
86-87	0.0	1.9409658024008083E-4	0.0	6.9312582005805154	0.0020102860096294084
88	0.0	1.9409658024008083E-4	0.0	7.383101175337978	0.002079606216858009
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6345	0.0	45.87326	1
GTATCAA	10865	0.0	38.214798	1
GGGCACC	3525	0.0	33.495304	94
GTACTAG	2745	0.0	31.861866	1
GTGGTAT	2200	0.0	30.991735	1
CAACGCA	13050	0.0	30.61161	5
ATCAACG	13155	0.0	30.377806	3
TAGTACT	4175	0.0	30.290892	4
TAGGCAT	3610	0.0	29.292273	5
TATCAAC	14390	0.0	28.783886	2
CTGTGCG	4400	0.0	28.414291	9
AACGCAG	14725	0.0	27.893936	6
CTAGTAC	4745	0.0	27.84187	3
GTACTGT	4725	0.0	27.650097	6
TCAACGC	15475	0.0	26.096289	4
TACTGTG	5580	0.0	25.182024	7
ACGCAGA	15770	0.0	25.12173	7
CTAGGCA	4230	0.0	25.006815	4
CGCAGAG	16130	0.0	24.590181	8
TATTCTC	1835	0.0	24.587418	5
>>END_MODULE