FastQCFastQC Report
Mon 16 Jan 2017
ERR1146453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780458
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC167970.35136800699849263No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN146000.3054100674035835No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA142730.29856971863365395No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT137910.2884870027097822No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC129000.26984862119905667No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA125670.26288276144252287No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC114290.23907751098325727No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC101160.21161152341470213No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG76460.15994283392930134No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC76310.1596290564627908No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT71620.14981828100989486No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT70260.14697336531353272No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC69290.14494427103009794No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT64820.13559370252808411No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT62640.13103347001479773No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA61750.1291717237135019No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT61410.12846049478941138No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC60460.12647323750151135No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC60450.126452319003744No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA58710.12281250039222183No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG58120.12157830902394709No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC58020.1213691240462734No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG57220.11969564422488391No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG56320.11781297942582071No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA52720.11028232022956795No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT52560.10994762426529006No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA52430.10967568379431428No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC51690.10812771495952898No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA50410.10545014724530578No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT49090.10268890554001311No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT48780.10204043210922469No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG48140.10070164825211309No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC49250.038.52105794
GTATCAA101400.032.3193441
GTACTAG48750.032.2095951
CAACGCA93250.031.7387815
TATTCTC28000.031.0393245
GGTATCA64900.030.2975181
ATCAACG98800.030.0091233
TAGTACT70650.028.9269814
CTGTGCG71800.028.1213959
TAGGCAT61700.027.8672735
CTAACGC25600.027.34813
TATCAAC120550.027.1675832
GCATCGT55300.026.8306948
ACTGTGC80000.026.8223138
AGGGCTA58100.026.6022475
CGCCTAC26350.026.372217
CTAGTAC80950.026.352383
GTACTGT76100.026.2197516
TCTAACG26750.025.997012
AACGCAG122150.025.8669916
Produced by FastQC (version 0.11.5)