##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146453_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4780458
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.96681112981225	31.0	30.0	34.0	26.0	34.0
2	30.030344372861347	31.0	30.0	34.0	26.0	34.0
3	30.261988914032923	31.0	30.0	34.0	26.0	34.0
4	33.71935283188347	35.0	35.0	37.0	28.0	37.0
5	33.92348682908625	35.0	35.0	37.0	28.0	37.0
6	34.17627265002642	37.0	35.0	37.0	30.0	37.0
7	33.985964315553026	36.0	35.0	37.0	29.0	37.0
8	33.96362335993748	36.0	35.0	37.0	29.0	37.0
9	35.293411007899245	39.0	35.0	39.0	28.0	39.0
10-11	35.207511497852295	38.0	35.0	39.0	27.5	39.0
12-13	35.194788762917696	38.0	35.0	39.0	27.0	39.0
14-15	36.12621374353671	39.0	35.0	41.0	27.0	41.0
16-17	35.946407227089956	39.0	35.0	41.0	27.0	41.0
18-19	35.88697735656291	39.0	34.5	41.0	27.0	41.0
20-21	36.23856343890063	39.0	35.0	41.0	27.0	41.0
22-23	35.93985775003148	39.0	34.5	41.0	27.0	41.0
24-25	36.03861314125132	39.0	35.0	41.0	27.0	41.0
26-27	36.11274599630412	39.0	35.0	41.0	27.0	41.0
28-29	35.95169354066075	39.0	34.5	41.0	27.0	41.0
30-31	35.88937723121927	39.0	34.0	41.0	27.0	41.0
32-33	36.00413966611568	39.0	35.0	41.0	27.0	41.0
34-35	36.049857984318656	39.0	35.0	41.0	27.5	41.0
36-37	35.96369929408438	39.0	35.0	41.0	27.0	41.0
38-39	35.79311135041873	39.0	34.0	41.0	27.0	41.0
40-41	35.74742305026004	39.0	34.0	41.0	27.0	41.0
42-43	35.576144796168066	38.0	34.0	40.0	26.5	41.0
44-45	35.37183947646857	38.0	33.5	40.0	26.0	41.0
46-47	35.23633133059636	38.0	33.0	40.0	26.0	41.0
48-49	35.10665860886132	38.0	33.0	40.0	26.0	41.0
50-51	34.883373203990075	38.0	33.0	40.0	25.5	41.0
52-53	34.68971477209924	37.0	33.0	40.0	25.0	41.0
54-55	34.496066485679826	37.0	33.0	40.0	25.0	41.0
56-57	34.33534558822606	36.5	32.5	40.0	25.0	41.0
58-59	34.06768378678361	36.0	32.0	40.0	25.0	41.0
60-61	33.818599075653424	35.5	32.0	39.0	24.5	41.0
62-63	33.551106191080436	35.0	32.0	39.0	24.0	41.0
64-65	33.2673569143375	35.0	31.0	39.0	23.5	40.5
66-67	32.98465722322004	35.0	31.0	38.0	23.0	40.0
68-69	32.7000290557934	35.0	31.0	37.5	23.0	40.0
70-71	32.658586373941574	35.0	31.0	37.0	23.5	40.0
72-73	32.46758856578177	35.0	31.0	36.5	23.0	40.0
74-75	32.2118860786979	35.0	31.0	36.0	23.0	39.0
76-77	31.99426655353943	35.0	31.0	36.0	23.0	39.0
78-79	31.791926212927713	34.5	30.0	35.5	23.0	39.0
80-81	31.59183283275368	34.0	30.0	35.0	22.5	37.0
82-83	31.367836094365856	34.0	30.0	35.0	22.0	37.0
84-85	31.195039784890902	34.0	30.0	35.0	21.0	36.5
86-87	31.044833361154936	34.0	30.0	35.0	21.0	36.0
88-89	31.06948413729396	34.0	30.0	35.0	21.0	36.0
90-91	31.00125709712333	34.0	30.0	35.0	21.0	36.0
92-93	30.866106030008005	34.0	30.0	35.0	20.0	35.5
94-95	30.85270082908374	34.0	30.0	35.0	20.0	35.0
96-97	30.771685160710543	34.0	30.0	35.0	20.0	35.0
98-99	30.701468980587215	34.0	30.0	35.0	20.0	35.0
100	30.177014001587295	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8960859465347184
1101	2	1.0725543025948987
1101	3	1.0600011959263895
1101	4	1.1542076550772649
1101	5	1.0824725941291646
1101	6	0.7672027723033708
1101	7	0.8423017989009125
1101	8	0.8766048247023406
1101	9	1.0748161993609884
1101	10-11	1.1089417742556265
1101	12-13	1.0193131333567464
1101	14-15	1.2880734632403374
1101	16-17	1.37276558496783
1101	18-19	1.5044258646886632
1101	20-21	1.1896238913331132
1101	22-23	1.497709623336931
1101	24-25	1.358926372505593
1101	26-27	1.2729787483160848
1101	28-29	1.4741003706625335
1101	30-31	1.5004913524448966
1101	32-33	1.3163942098941916
1101	34-35	1.2177890841256414
1101	36-37	1.1481477154762345
1101	38-39	1.2965624129127562
1101	40-41	1.195765569259116
1101	42-43	1.2374892277489877
1101	44-45	1.2620425568518172
1101	46-47	1.2847321388930553
1101	48-49	1.2482527542115704
1101	50-51	1.2277412027072785
1101	52-53	1.3001700965102572
1101	54-55	1.2806245530229958
1101	56-57	1.2049013904812753
1101	58-59	1.0417829728529782
1101	60-61	1.1286349751491187
1101	62-63	1.0448973509912847
1101	64-65	1.0351912105575352
1101	66-67	1.093904596208148
1101	68-69	1.0593423402279285
1101	70-71	1.015312276382673
1101	72-73	0.9781966548613923
1101	74-75	1.0287553962993528
1101	76-77	0.9648312966514077
1101	78-79	0.9671199661286423
1101	80-81	1.0107308465050977
1101	82-83	1.0181651617754888
1101	84-85	0.9881982837463781
1101	86-87	0.9683626619314616
1101	88-89	0.9325370013036611
1101	90-91	0.9883239493585236
1101	92-93	1.0685345829418509
1101	94-95	1.037050166140368
1101	96-97	0.9843540101876584
1101	98-99	0.9767511701940315
1101	100	1.021704118230378
1102	1	0.8568288592727349
1102	2	0.9964170901541358
1102	3	1.0192414253012565
1102	4	1.1595183378665297
1102	5	0.9999706326063986
1102	6	0.729404340624896
1102	7	0.8091696841776113
1102	8	0.8439712730235058
1102	9	1.0406270592477398
1102	10-11	1.0231544078073114
1102	12-13	0.9594218577616758
1102	14-15	1.1865021826237694
1102	16-17	1.3189925566905174
1102	18-19	1.4020956653082877
1102	20-21	1.149660880048188
1102	22-23	1.3640275200464984
1102	24-25	1.2969372314956473
1102	26-27	1.1315171784701832
1102	28-29	1.3237067658992814
1102	30-31	1.3791903808746468
1102	32-33	1.159346806053641
1102	34-35	1.1140634910732388
1102	36-37	1.0914774620250753
1102	38-39	1.1178884471396415
1102	40-41	1.026848823010667
1102	42-43	1.0760807423135716
1102	44-45	1.1483666407646425
1102	46-47	1.088068969341272
1102	48-49	1.0082931503719124
1102	50-51	1.1083855685413653
1102	52-53	1.094224732205042
1102	54-55	1.1437138056716023
1102	56-57	0.9761804378313101
1102	58-59	0.9644193939594103
1102	60-61	0.9985602355272576
1102	62-63	0.9815399862018523
1102	64-65	0.9264928994846855
1102	66-67	0.9873859740714153
1102	68-69	0.996454171684924
1102	70-71	0.9279266387644824
1102	72-73	0.873609022991019
1102	74-75	0.8462471579037256
1102	76-77	0.8693918931336633
1102	78-79	0.8657096424661717
1102	80-81	0.9289202825280753
1102	82-83	0.9733022441953132
1102	84-85	0.9429648064933147
1102	86-87	0.9258982719762514
1102	88-89	0.9005469187627817
1102	90-91	0.8971107195039245
1102	92-93	0.9371023350591727
1102	94-95	0.9408679843890297
1102	96-97	0.937546293220823
1102	98-99	1.0118046805351781
1102	100	0.9531865810959488
1103	1	0.6716868594870746
1103	2	0.8819054010073586
1103	3	0.8259196254635413
1103	4	0.8741929248806244
1103	5	0.8714778971776411
1103	6	0.5757322491326349
1103	7	0.6774404253322857
1103	8	0.7140608362306295
1103	9	0.778728319256615
1103	10-11	0.9268777876318524
1103	12-13	0.7507827592115319
1103	14-15	0.977368572690672
1103	16-17	1.1958971910123637
1103	18-19	1.2115856908759
1103	20-21	0.9723545126400097
1103	22-23	1.1571913212008695
1103	24-25	1.0568579259549722
1103	26-27	0.9821756594169528
1103	28-29	1.1260519440305572
1103	30-31	1.1665318080681715
1103	32-33	1.0002598914678558
1103	34-35	1.0304959665390427
1103	36-37	0.9574706950272045
1103	38-39	1.0095726947321495
1103	40-41	1.0045777450370892
1103	42-43	0.9729807836504492
1103	44-45	0.9962543269719077
1103	46-47	0.9230185230562284
1103	48-49	0.9420601635537835
1103	50-51	0.9618678166544115
1103	52-53	0.981073791408619
1103	54-55	0.9399463331912372
1103	56-57	0.9263220548682298
1103	58-59	0.8955628941201681
1103	60-61	0.8962162821919861
1103	62-63	0.8438710791791806
1103	64-65	0.8900423582779524
1103	66-67	0.9514770988934345
1103	68-69	0.9389849064094165
1103	70-71	0.8267165403214989
1103	72-73	0.8517902009385665
1103	74-75	0.8223275045209917
1103	76-77	0.7729131816190566
1103	78-79	0.8311599846435556
1103	80-81	0.8572654300394476
1103	82-83	0.7801111913300609
1103	84-85	0.7755633642960298
1103	86-87	0.805050713147768
1103	88-89	0.7695702970563332
1103	90-91	0.8331071675955393
1103	92-93	0.9105558004663408
1103	94-95	0.8571691004843807
1103	96-97	0.879193643833986
1103	98-99	0.9134988795951458
1103	100	0.9870589267333791
1104	1	0.6945974576048535
1104	2	0.8327191854799487
1104	3	0.8024057602694619
1104	4	0.901111758443065
1104	5	0.8518870861856627
1104	6	0.5377623586263809
1104	7	0.5889339924846411
1104	8	0.624204402473886
1104	9	0.7214871417588711
1104	10-11	0.6918939662359662
1104	12-13	0.6306388104588692
1104	14-15	0.8807619628595234
1104	16-17	0.9937063126438375
1104	18-19	0.9636196889293913
1104	20-21	0.8299223240103899
1104	22-23	1.0584236704501393
1104	24-25	0.9167796882015438
1104	26-27	0.8683833393824827
1104	28-29	1.0128027530465218
1104	30-31	1.0184687979627256
1104	32-33	0.839846261577101
1104	34-35	0.7601896399706973
1104	36-37	0.8465135844517917
1104	38-39	0.9533591553400314
1104	40-41	0.7915058720343282
1104	42-43	0.7226504361775241
1104	44-45	0.7929113319884422
1104	46-47	0.7854573429924159
1104	48-49	0.7208453171115821
1104	50-51	0.9261912501411729
1104	52-53	0.9276673907741042
1104	54-55	0.8586142244597639
1104	56-57	0.6878218485189862
1104	58-59	0.6675195343633789
1104	60-61	0.6491862104345998
1104	62-63	0.5809036844181534
1104	64-65	0.6669117915225087
1104	66-67	0.7725783846396155
1104	68-69	0.7262067594633734
1104	70-71	0.6386878325617715
1104	72-73	0.6084424425980615
1104	74-75	0.6245484776317767
1104	76-77	0.7033983922273599
1104	78-79	0.6777488326590202
1104	80-81	0.7084520153831235
1104	82-83	0.6182797380104468
1104	84-85	0.620857855371554
1104	86-87	0.5229513900350433
1104	88-89	0.49351594332741655
1104	90-91	0.5695551595240751
1104	92-93	0.5521040836166122
1104	94-95	0.46916090757888185
1104	96-97	0.5481024902988274
1104	98-99	0.6642603563353155
1104	100	0.7098703640047503
1105	1	0.5124256260058253
1105	2	0.6418224356673257
1105	3	0.6510446258556115
1105	4	0.7835797425015087
1105	5	0.6363051026717699
1105	6	0.33799103505619854
1105	7	0.43421884974360125
1105	8	0.5260502967119649
1105	9	0.5156407686230509
1105	10-11	0.5700020429180839
1105	12-13	0.5402506550124144
1105	14-15	0.7341068056467179
1105	16-17	0.8226628879843005
1105	18-19	0.7956029614395845
1105	20-21	0.6876630397804462
1105	22-23	0.9243928894081961
1105	24-25	0.782411458045928
1105	26-27	0.5961106633951516
1105	28-29	0.8889012385324193
1105	30-31	0.9011768860655636
1105	32-33	0.7536952545814941
1105	34-35	0.6765870186124658
1105	36-37	0.5801237161735031
1105	38-39	0.6978799348398965
1105	40-41	0.5449676047669101
1105	42-43	0.6514137420726271
1105	44-45	0.6715684789992835
1105	46-47	0.551395554976942
1105	48-49	0.62654973817083
1105	50-51	0.7268526862661133
1105	52-53	0.7057655639833555
1105	54-55	0.5638604581745597
1105	56-57	0.591821920519159
1105	58-59	0.5595483007796318
1105	60-61	0.6301125079067802
1105	62-63	0.5922779885198679
1105	64-65	0.49898571943019476
1105	66-67	0.6058574534704135
1105	68-69	0.588430555329353
1105	70-71	0.5110660329490244
1105	72-73	0.41644417280924984
1105	74-75	0.45110656425874396
1105	76-77	0.4529944601900091
1105	78-79	0.47401232683045436
1105	80-81	0.549135481907598
1105	82-83	0.5522340912764214
1105	84-85	0.5175038056649477
1105	86-87	0.5938235659935494
1105	88-89	0.5883280752747773
1105	90-91	0.5886059999261626
1105	92-93	0.5640255959734688
1105	94-95	0.49476179872592496
1105	96-97	0.5230916695823069
1105	98-99	0.5243649745260939
1105	100	0.6100913576568736
1106	1	0.6258520720800966
1106	2	0.6765405190696185
1106	3	0.6441959995897655
1106	4	0.7796398314854258
1106	5	0.6050553018319533
1106	6	0.4156772819531511
1106	7	0.4004101017716053
1106	8	0.486663915670988
1106	9	0.6367173300817441
1106	10-11	0.5523376051322941
1106	12-13	0.5458984352971896
1106	14-15	0.7782470675273743
1106	16-17	0.8764186122335289
1106	18-19	0.7609127690294244
1106	20-21	0.6897563546796803
1106	22-23	1.0168353128407404
1106	24-25	0.7722484379380319
1106	26-27	0.6418551027570771
1106	28-29	0.8825830036827256
1106	30-31	0.873553076957819
1106	32-33	0.7599977108048037
1106	34-35	0.6732515266396888
1106	36-37	0.6498477272682237
1106	38-39	0.6950745259062145
1106	40-41	0.6415418097108798
1106	42-43	0.6664481261151494
1106	44-45	0.696589285885004
1106	46-47	0.6451903231887499
1106	48-49	0.6715517597538039
1106	50-51	0.6609505891307705
1106	52-53	0.6571164953677453
1106	54-55	0.7125482195455319
1106	56-57	0.6700040462993115
1106	58-59	0.66444154235365
1106	60-61	0.5583004757190722
1106	62-63	0.5787307956934029
1106	64-65	0.5964820403205167
1106	66-67	0.6055448619209756
1106	68-69	0.5848484131024207
1106	70-71	0.6078838472066721
1106	72-73	0.5907652121770113
1106	74-75	0.5222176553824553
1106	76-77	0.5831206720690396
1106	78-79	0.6298040849394866
1106	80-81	0.6407303224166867
1106	82-83	0.6253277471036363
1106	84-85	0.5634589522211471
1106	86-87	0.6074379079254619
1106	88-89	0.6177757890871582
1106	90-91	0.6129564978694582
1106	92-93	0.6555319493975702
1106	94-95	0.6567374163794817
1106	96-97	0.6762191098299688
1106	98-99	0.663119499531188
1106	100	0.6323952598259623
1107	1	0.5187677201441723
1107	2	0.6484547158675191
1107	3	0.6180905409146362
1107	4	0.735630559938258
1107	5	0.7158077613530125
1107	6	0.46759598000441827
1107	7	0.43490755235482936
1107	8	0.4931637772703823
1107	9	0.663196237570638
1107	10-11	0.6128820498354344
1107	12-13	0.5343764942739568
1107	14-15	0.7557658680209585
1107	16-17	0.8610860756737821
1107	18-19	0.9568519400227373
1107	20-21	0.7349872767381527
1107	22-23	0.9295265449683114
1107	24-25	0.6382206698612336
1107	26-27	0.6207262453252298
1107	28-29	0.8078567393867218
1107	30-31	0.8135118672071684
1107	32-33	0.7003826197061613
1107	34-35	0.6169939307746333
1107	36-37	0.6165885093998043
1107	38-39	0.604579103473931
1107	40-41	0.4510634092597172
1107	42-43	0.6170904860136375
1107	44-45	0.5231079285760316
1107	46-47	0.49022458703979765
1107	48-49	0.5032976251973693
1107	50-51	0.5506312847037549
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2209	22-23	0.5596505159669505
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2209	26-27	0.32483313525187896
2209	28-29	0.36644495653229825
2209	30-31	0.4260639308194385
2209	32-33	0.30825772313219346
2209	34-35	0.12380953092609559
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2210	2	-0.23147071017586285
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2210	4	-0.1165259762888482
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2210	7	-0.7559404016330618
2210	8	-0.4823649772480323
2210	9	-0.20734525343873855
2210	10-11	-0.0918492875599739
2210	12-13	-0.14990718202686537
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2210	18-19	0.19904288437108875
2210	20-21	-0.472121249164033
2210	22-23	-1.86358778528664
2210	24-25	-0.9191456035548384
2210	26-27	-0.26357407126143073
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2210	30-31	0.03287483642456124
2210	32-33	-0.08791018940896578
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2210	36-37	-0.025092833776575674
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2211	10-11	-0.16749452822020316
2211	12-13	-0.0989835919953066
2211	14-15	-0.2087917456458399
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2211	22-23	-2.154252640745476
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2211	28-29	-0.14713461970187325
2211	30-31	-0.08136340350492333
2211	32-33	-0.26244672202458474
2211	34-35	-0.32947898510531104
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2212	58-59	-0.604484183680789
2212	60-61	-0.45152181769890376
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2212	86-87	-0.30678183741666487
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2212	94-95	-0.37738312068264435
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2212	98-99	-0.40934406463106754
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2213	1	-0.7004200115324473
2213	2	-0.5222424342775334
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2213	4	-0.6944098913817953
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2213	22-23	-0.7205438214244992
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2213	26-27	-3.1730809774893487
2213	28-29	-1.1011618991000276
2213	30-31	-1.5706291345981285
2213	32-33	-0.8894780225451555
2213	34-35	-0.7830155961142893
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2213	64-65	-0.7171949948002805
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2213	68-69	-0.7238862043400616
2213	70-71	-0.7535028036683613
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2213	82-83	-0.6691602715138671
2213	84-85	-0.587106118139932
2213	86-87	-0.5694754510692768
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2213	90-91	-0.7556748765740693
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2213	94-95	-0.7394978374034977
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2213	100	-0.5676243238774852
2214	1	-0.8829992459883229
2214	2	-0.9168432912259874
2214	3	-1.137687379029824
2214	4	-0.8687745139295373
2214	5	-1.1607725892055996
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2214	8	-1.0245867795127808
2214	9	-4.549799759521488
2214	10-11	-1.8582438862950923
2214	12-13	-1.448583182719858
2214	14-15	-3.9271388720167977
2214	16-17	-3.3327746567986125
2214	18-19	-1.8248825741662458
2214	20-21	-1.5534626507175915
2214	22-23	-2.8103222197733047
2214	24-25	-2.2004535626022204
2214	26-27	-1.5123501631606402
2214	28-29	-1.0815697990333888
2214	30-31	-1.1225984916415968
2214	32-33	-1.2627010323029495
2214	34-35	-1.108269720824545
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2214	38-39	-1.167555943534829
2214	40-41	-1.3230172716401896
2214	42-43	-3.8992251030862803
2214	44-45	-1.7889559500537402
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2214	50-51	-1.1744424479086177
2214	52-53	-1.1756302882486906
2214	54-55	-1.0990585951092058
2214	56-57	-0.9163120358833297
2214	58-59	-1.2006402907069074
2214	60-61	-1.3531887535671032
2214	62-63	-1.2507102235916534
2214	64-65	-1.1995777905048612
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2214	70-71	-1.02831838373735
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2214	80-81	-1.0193413675816672
2214	82-83	-1.1005760447596664
2214	84-85	-1.1588571617413237
2214	86-87	-1.0286131039972695
2214	88-89	-0.9830687069112294
2214	90-91	-0.9456360529385428
2214	92-93	-1.0531841984633843
2214	94-95	-1.1722608569164592
2214	96-97	-1.0970304042034726
2214	98-99	-1.2482430871350552
2214	100	-1.1466035166506074
2215	1	-4.316181206054289
2215	2	-2.4934218570327467
2215	3	-4.031106625406313
2215	4	-2.307815497059078
2215	5	-1.7946693864970698
2215	6	-1.637481851626454
2215	7	-1.4907036985189706
2215	8	-1.4287538702050924
2215	9	-2.050628671317618
2215	10-11	-1.6448599406376019
2215	12-13	-1.5098513103999807
2215	14-15	-3.9066785062001372
2215	16-17	-3.6824682613091895
2215	18-19	-2.640685831243424
2215	20-21	-2.1581802200687434
2215	22-23	-3.5643576213248167
2215	24-25	-5.916670271920424
2215	26-27	-3.9816334337693036
2215	28-29	-2.0766352296925206
2215	30-31	-4.312985736763274
2215	32-33	-4.378745027026049
2215	34-35	-3.3504418182872655
2215	36-37	-2.2741022466091962
2215	38-39	-2.1483406181741103
2215	40-41	-2.3894123751631398
2215	42-43	-2.132259720073634
2215	44-45	-3.5666477815924758
2215	46-47	-3.068924499851555
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2215	50-51	-5.402039227678877
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2215	54-55	-3.377991927073211
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2215	58-59	-1.9382604922536828
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2215	62-63	-3.0502108614195933
2215	64-65	-2.136287488383857
2215	66-67	-1.8871354254089283
2215	68-69	-1.9934269244142158
2215	70-71	-1.7847743882362224
2215	72-73	-1.7152676819647006
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2215	80-81	-1.8999768240931303
2215	82-83	-1.8129942233695395
2215	84-85	-1.6907582842500375
2215	86-87	-1.687479838259069
2215	88-89	-1.5733576169832482
2215	90-91	-1.98789686123569
2215	92-93	-4.16099676605463
2215	94-95	-2.3671050395490525
2215	96-97	-1.9713259770022233
2215	98-99	-3.4710705897655707
2215	100	-3.404953787669129
2216	1	-0.952226930755451
2216	2	-0.7810296183328411
2216	3	-1.1371818888568548
2216	4	-1.1553325592273893
2216	5	-1.1601627614496337
2216	6	-1.064020293622292
2216	7	-1.1953220605436101
2216	8	-1.0178845329548807
2216	9	-1.3420318393908275
2216	10-11	-1.332675233544009
2216	12-13	-1.6019569742218351
2216	14-15	-2.0368652197809354
2216	16-17	-1.8136414617319616
2216	18-19	-3.6358883970821907
2216	20-21	-1.9062439581097266
2216	22-23	-1.3582613758847302
2216	24-25	-1.5068790431668262
2216	26-27	-1.4967420878625077
2216	28-29	-1.5294332307008034
2216	30-31	-1.6122617905564454
2216	32-33	-1.5370517957852456
2216	34-35	-1.821073114010261
2216	36-37	-1.7988636519286203
2216	38-39	-1.9495768978989005
2216	40-41	-2.0602855448471544
2216	42-43	-1.8908937772390644
2216	44-45	-1.7251407666349934
2216	46-47	-1.9215062935747156
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2216	50-51	-1.8173123052547595
2216	52-53	-1.8300916547384531
2216	54-55	-1.632618550362082
2216	56-57	-1.7175485094763516
2216	58-59	-1.6880716183117457
2216	60-61	-1.758071218270672
2216	62-63	-1.7693844539145829
2216	64-65	-1.7610751459649947
2216	66-67	-1.7666865531751057
2216	68-69	-1.7764960804751766
2216	70-71	-1.8737214473162425
2216	72-73	-1.8206283055180776
2216	74-75	-1.9320218779275677
2216	76-77	-1.8478624475248644
2216	78-79	-1.9264915378487544
2216	80-81	-1.8548474384609221
2216	82-83	-1.923438227133147
2216	84-85	-1.8023202613894824
2216	86-87	-1.9129276672492814
2216	88-89	-1.8311547369101397
2216	90-91	-1.8408176640900358
2216	92-93	-1.7585901345567407
2216	94-95	-1.690988849957634
2216	96-97	-1.7216852113659087
2216	98-99	-1.7351481173610743
2216	100	-1.7821413109799487
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	19768.0
1	2861.0
2	509.0
3	532.0
4	419.0
5	255.0
6	266.0
7	341.0
8	614.0
9	1086.0
10	2254.0
11	5040.0
12	9158.0
13	13104.0
14	16568.0
15	19939.0
16	23098.0
17	26252.0
18	29780.0
19	33076.0
20	36970.0
21	41710.0
22	47006.0
23	52771.0
24	59404.0
25	66230.0
26	74588.0
27	85189.0
28	99187.0
29	115981.0
30	136616.0
31	162221.0
32	195571.0
33	246145.0
34	333588.0
35	436122.0
36	630208.0
37	881035.0
38	665382.0
39	209507.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23896603493904	21.30180315129983	12.498315078431778	23.96091573532935
2	15.379493182598033	19.8652215947188	40.925025073170794	23.830260149512373
3	18.468821637882943	27.12809047902714	28.74406665441917	25.65902122867075
4	12.155811413394938	16.745742165325694	40.18813348406542	30.910312937213945
5	13.144282570613031	38.686389025803024	34.86829139978915	13.301037003794796
6	29.534942525981382	38.61429675540368	18.383572522023456	13.467188196591476
7	26.75761011400902	31.7774882697753	23.979713790716268	17.485187825499413
8	26.150343007394145	34.968905330127406	20.03259468994995	18.848156972528503
9	26.341604445836865	14.849860489370068	21.77871322207527	37.029821842717794
10-11	25.762977403036828	25.77111571651331	27.468311573333672	20.99759530711619
12-13	25.920001985135453	23.70687035614466	27.48147715106986	22.891650507650027
14-15	23.373558961342916	24.82039883565601	24.87941511533829	26.92662708766278
16-17	22.32992160665714	27.272875432318934	26.35341994730317	24.043783013720756
18-19	21.72731812107804	28.051683633680728	27.947853309358955	22.27314493588228
20-21	23.52287743605833	27.178174078127142	26.610769840275157	22.68817864553937
22-23	24.119207553238116	26.77438138050709	26.310312970014703	22.79609809624009
24-25	23.414241277087672	26.920638859259498	27.145977584937043	22.519142278715783
26-27	23.212118937716568	27.131480054775693	26.145647961590164	23.510753045917582
28-29	22.39036588996557	27.714970657148676	26.721464098108903	23.173199354776855
30-31	23.20948206223058	27.288125828680666	26.611325270907354	22.891066838181402
32-33	23.664350859184097	27.576734887019622	25.566731179022263	23.19218307477402
34-35	23.614130794787286	27.26499056961462	26.64843535932933	22.472443276268763
36-37	23.277262195614426	26.88712957167279	26.378915872471026	23.45669236024176
38-39	23.53838679963491	26.85956105337096	26.98746798120723	22.614584165786894
40-41	23.02510558060579	26.074468858124757	27.60335153291546	23.297074028353997
42-43	23.004853902875887	26.299007105085803	26.904517266482625	23.79162172555569
44-45	23.102874068016497	27.33951837212707	26.73573777084522	22.821869789011217
46-47	23.107974080339925	26.96515198727595	26.320883305266708	23.60599062711742
48-49	23.804692622028735	26.474870926190007	26.60342957639567	23.11700687538558
50-51	23.39288170281923	26.45252723061387	26.42279089975744	23.73180016680946
52-53	22.818016362261865	27.102180548443837	26.298882020753013	23.780921068541286
54-55	22.80978232524021	26.996904721445965	26.288374471922516	23.904938481391312
56-57	23.937445373100996	26.348740962018002	26.075828575652203	23.637985089228795
58-59	22.839574322403298	26.720603969940292	27.05577481801496	23.384046889641446
60-61	22.83266117373596	27.448356841571155	26.270211312341935	23.448770672350953
62-63	23.352726829258035	26.632218154751264	25.864349259549734	24.15070575644096
64-65	22.40429539742047	26.840629631323637	26.37549819176302	24.379576779492872
66-67	23.24415986938623	26.328940742981473	26.29415919120906	24.132740196423242
68-69	22.559980447599788	26.53120417734843	26.55375169566487	24.35506367938691
70-71	22.526643475203343	26.129153822827146	26.81263745484415	24.531565247125357
72-73	23.213633023641012	26.26453141738811	27.161579224747122	23.360256334223756
74-75	23.31887216561165	26.624594373866557	26.725590168214513	23.330943292307275
76-77	22.962183164383074	26.545380223767534	26.355146907767647	24.13728970408174
78-79	22.759274039315528	26.571439478894753	26.50353223869148	24.165754243098245
80-81	22.390549503813205	26.8289413297291	27.207922166199577	23.57258700025812
82-83	23.235255710260017	25.71211470293779	28.23247052529913	22.820159061503066
84-85	23.566783474806265	26.373585700042334	26.673916440295255	23.385714384856147
86-87	22.119453363708345	27.01056287185402	27.266621305956022	23.60336245848161
88-89	23.186534524966294	25.708640749058663	27.06133691945648	24.04348780651856
90-91	22.760421882397306	25.74905338289183	27.39134034764617	24.099184387064696
92-93	23.092846909593725	26.0190179173052	27.225091248864807	23.663043924236266
94-95	21.82959409646386	25.9638502424292	27.965433759972203	24.24112190113474
96-97	22.2221529012924	25.93514521794853	27.401980792697756	24.440721088061316
98-99	21.583685015318004	26.67976501640924	27.31012265718786	24.42642731108489
100	22.398077325680983	25.813425308162905	26.58103590105798	25.20746146509813
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16244.0
1	9497.5
2	2540.0
3	1801.0
4	931.5
5	490.5
6	373.5
7	355.5
8	350.5
9	329.0
10	319.5
11	311.5
12	295.0
13	309.0
14	331.0
15	434.5
16	515.5
17	536.0
18	666.5
19	838.5
20	1030.5
21	1335.5
22	1827.0
23	2467.5
24	3361.5
25	4657.0
26	6792.0
27	9330.5
28	13586.0
29	22918.5
30	36225.5
31	48992.0
32	65579.0
33	81767.5
34	94605.0
35	108617.0
36	120766.0
37	143916.0
38	154121.5
39	142616.5
40	131824.5
41	121368.0
42	119066.5
43	127190.0
44	140815.5
45	168659.0
46	196543.0
47	215042.5
48	225347.5
49	220068.5
50	210546.0
51	216623.5
52	221139.0
53	213758.5
54	203031.5
55	176580.5
56	154674.5
57	129455.0
58	103927.0
59	97511.5
60	81657.0
61	58738.5
62	44711.5
63	36677.5
64	26894.5
65	14282.0
66	8230.5
67	5725.0
68	4224.0
69	3005.0
70	2186.5
71	1690.5
72	1349.0
73	1100.5
74	854.5
75	660.0
76	485.0
77	298.5
78	181.5
79	103.5
80	64.5
81	54.5
82	37.5
83	35.5
84	31.0
85	22.5
86	21.5
87	18.0
88	14.5
89	12.5
90	11.0
91	9.5
92	8.0
93	6.5
94	5.0
95	4.0
96	1.5
97	1.5
98	2.5
99	2.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9894449443965411
2	0.4473839117507151
3	0.48162749259589765
4	0.46746566960738906
5	0.4347700575969918
6	0.4793892133347893
7	0.49637503352189266
8	0.49901076424058116
9	0.5135072831933677
10-11	0.5265813442979731
12-13	0.5253680714274657
14-15	0.5227637184554284
16-17	0.5259642486138357
18-19	0.525127508703141
20-21	0.5235899991172394
22-23	0.4872545684953199
24-25	0.5219269785447336
26-27	0.5013850137371775
28-29	0.5077756148051086
30-31	0.5104950195148666
32-33	0.5129529430025325
34-35	0.5247405164944448
36-37	0.543086039036427
38-39	0.5321247462063259
40-41	0.537155644919378
42-43	0.5360260460399401
44-45	0.5455962587685114
46-47	0.5438286457071686
48-49	0.5156828069611741
50-51	0.5299073854429848
52-53	0.5230251996775204
54-55	0.5328778121259511
56-57	0.5209228906518999
58-59	0.5255145009118373
60-61	0.5206823279275751
62-63	0.5139256531487151
64-65	0.5257969006316968
66-67	0.5242907687924463
68-69	0.5450210000799087
70-71	0.5284012536037342
72-73	0.5316645392554437
74-75	0.529436719243219
76-77	0.5170843463115877
78-79	0.5476148938030624
80-81	0.5217177935670599
82-83	0.5216864158204089
84-85	0.5306395328648427
86-87	0.8354534230820561
88-89	1.1003966565546648
90-91	1.0913912432658126
92-93	1.0915272135013003
94-95	1.0956376983125884
96-97	1.0887659717960079
98-99	1.0850947754378346
100	1.0799802027337129
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4780458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.888614766668873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.79125621045166	20.858245323685175
2	11.674402801242477	6.511658447095428
3	4.784730886052455	4.003185493298974
4	2.565454571371433	2.8618789696947005
5	1.4493312994163727	2.0209921139349416
6	0.9422549643102666	1.5766911426978212
7	0.6581324149131104	1.284808096947845
8	0.46323904779636393	1.0335276279097043
9	0.3355444793011285	0.8422083648280504
>10	1.823684391321027	9.413236047906025
>50	0.19794883516919973	3.8779592197691852
>100	0.23124550510929898	14.507016166536435
>500	0.045102022412858475	8.869019742263884
>1k	0.035496267222396176	17.424606773111147
>5k	0.0015759442107194921	2.6865930033030234
>10k+	6.003596993217112E-4	2.228373467017869
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	16797	0.35136800699849263	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14600	0.3054100674035835	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14273	0.29856971863365395	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13791	0.2884870027097822	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	12900	0.26984862119905667	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12567	0.26288276144252287	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11429	0.23907751098325727	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10116	0.21161152341470213	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7646	0.15994283392930134	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7631	0.1596290564627908	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7162	0.14981828100989486	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7026	0.14697336531353272	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6929	0.14494427103009794	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6482	0.13559370252808411	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6264	0.13103347001479773	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6175	0.1291717237135019	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6141	0.12846049478941138	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6046	0.12647323750151135	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6045	0.126452319003744	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5871	0.12281250039222183	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5812	0.12157830902394709	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5802	0.1213691240462734	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5722	0.11969564422488391	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5632	0.11781297942582071	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5272	0.11028232022956795	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5256	0.10994762426529006	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5243	0.10967568379431428	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5169	0.10812771495952898	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5041	0.10545014724530578	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4909	0.10268890554001311	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4878	0.10204043210922469	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4814	0.10070164825211309	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.0918497767368734E-5	0.0
6	0.0	0.0	0.0	2.0918497767368734E-5	0.0
7	0.0	0.0	0.0	2.0918497767368734E-5	0.0
8	0.0	0.0	0.0	2.0918497767368734E-5	0.0
9	0.0	0.0	0.0	2.0918497767368734E-5	0.0
10-11	0.0	0.0	0.0	2.0918497767368734E-5	0.0
12-13	0.0	0.0	0.0	2.0918497767368734E-5	0.0
14-15	0.0	0.0	0.0	2.0918497767368734E-5	1.0459248883684367E-5
16-17	0.0	0.0	0.0	2.0918497767368734E-5	2.0918497767368734E-5
18-19	0.0	0.0	0.0	4.183699553473747E-5	2.0918497767368734E-5
20-21	0.0	0.0	0.0	5.229624441842184E-5	2.0918497767368734E-5
22-23	0.0	0.0	0.0	1.4642948437158115E-4	4.183699553473747E-5
24-25	0.0	0.0	0.0	2.196442265573717E-4	4.183699553473747E-5
26-27	0.0	0.0	0.0	5.752586886026401E-4	4.183699553473747E-5
28-29	0.0	0.0	0.0	0.0017153168169242363	4.183699553473747E-5
30-31	0.0	0.0	0.0	0.006505652805651676	4.183699553473747E-5
32-33	0.0	0.0	0.0	0.01834552254198238	4.183699553473747E-5
34-35	0.0	0.0	0.0	0.036377267617454224	4.183699553473747E-5
36-37	0.0	0.0	1.0459248883684367E-5	0.0694494125876642	4.183699553473747E-5
38-39	0.0	0.0	2.0918497767368734E-5	0.12240458968575815	4.183699553473747E-5
40-41	0.0	0.0	2.0918497767368734E-5	0.1818445010917364	4.183699553473747E-5
42-43	0.0	0.0	2.0918497767368734E-5	0.24598061524648895	4.183699553473747E-5
44-45	0.0	0.0	2.0918497767368734E-5	0.3230443610214754	5.229624441842184E-5
46-47	0.0	0.0	2.0918497767368734E-5	0.41897659178262836	7.321474218579058E-5
48-49	0.0	0.0	2.0918497767368734E-5	0.5344989957029221	8.367399106947494E-5
50-51	0.0	0.0	2.0918497767368734E-5	0.6602819227781104	8.367399106947494E-5
52-53	0.0	0.0	2.0918497767368734E-5	0.7840462148187475	8.367399106947494E-5
54-55	0.0	0.0	2.0918497767368734E-5	0.9220350853411954	8.367399106947494E-5
56-57	0.0	0.0	2.0918497767368734E-5	1.091182058288139	8.367399106947494E-5
58-59	0.0	0.0	2.0918497767368734E-5	1.298160134447369	8.367399106947494E-5
60-61	0.0	0.0	2.0918497767368734E-5	1.5143841866197758	8.367399106947494E-5
62-63	0.0	0.0	2.0918497767368734E-5	1.7443412325764602	8.367399106947494E-5
64-65	0.0	0.0	2.0918497767368734E-5	1.9857720745585465	8.367399106947494E-5
66-67	0.0	0.0	2.0918497767368734E-5	2.261195475412607	8.367399106947494E-5
68-69	0.0	0.0	2.0918497767368734E-5	2.575129830656393	8.367399106947494E-5
70-71	0.0	0.0	2.0918497767368734E-5	2.925368238775448	8.367399106947494E-5
72-73	0.0	0.0	2.0918497767368734E-5	3.291400112708866	1.3597023548789678E-4
74-75	0.0	0.0	2.0918497767368734E-5	3.646564827052136	1.882664799063186E-4
76-77	0.0	0.0	2.0918497767368734E-5	4.064181716479886	1.882664799063186E-4
78-79	0.0	0.0	2.0918497767368734E-5	4.512004916683715	1.882664799063186E-4
80-81	0.0	0.0	2.0918497767368734E-5	5.000326328565171	1.882664799063186E-4
82-83	0.0	0.0	2.0918497767368734E-5	5.514722647913652	1.882664799063186E-4
84-85	0.0	0.0	2.0918497767368734E-5	6.016212254139666	1.882664799063186E-4
86-87	0.0	0.0	2.0918497767368734E-5	6.553462032298997	1.9872572879000297E-4
88	0.0	0.0	2.0918497767368734E-5	6.986506313830181	2.0918497767368732E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4925	0.0	38.521057	94
GTATCAA	10140	0.0	32.319344	1
GTACTAG	4875	0.0	32.209595	1
CAACGCA	9325	0.0	31.738781	5
TATTCTC	2800	0.0	31.039324	5
GGTATCA	6490	0.0	30.297518	1
ATCAACG	9880	0.0	30.009123	3
TAGTACT	7065	0.0	28.926981	4
CTGTGCG	7180	0.0	28.121395	9
TAGGCAT	6170	0.0	27.867273	5
CTAACGC	2560	0.0	27.3481	3
TATCAAC	12055	0.0	27.167583	2
GCATCGT	5530	0.0	26.830694	8
ACTGTGC	8000	0.0	26.822313	8
AGGGCTA	5810	0.0	26.602247	5
CGCCTAC	2635	0.0	26.37221	7
CTAGTAC	8095	0.0	26.35238	3
GTACTGT	7610	0.0	26.219751	6
TCTAACG	2675	0.0	25.99701	2
AACGCAG	12215	0.0	25.866991	6
>>END_MODULE