##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4780458
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.768652292311742	31.0	31.0	34.0	30.0	34.0
2	31.721895475287095	31.0	31.0	34.0	30.0	34.0
3	31.909162051000134	31.0	31.0	34.0	30.0	34.0
4	35.672124721104126	37.0	35.0	37.0	33.0	37.0
5	35.58427121418073	37.0	35.0	37.0	33.0	37.0
6	35.62227656847942	37.0	35.0	37.0	33.0	37.0
7	35.532461952390335	37.0	35.0	37.0	33.0	37.0
8	35.51599344665302	37.0	35.0	37.0	33.0	37.0
9	37.08357023532055	39.0	37.0	39.0	33.0	39.0
10-11	37.02667359487313	39.0	37.0	39.0	33.0	39.0
12-13	36.96824279179945	39.0	37.0	39.0	33.0	39.0
14-15	38.149120021554424	40.0	37.0	41.0	33.0	41.0
16-17	38.15136258910757	40.0	37.0	41.0	33.0	41.0
18-19	38.19449391669166	40.0	37.0	41.0	33.0	41.0
20-21	38.207918048856406	40.0	37.0	41.0	33.0	41.0
22-23	38.1737833487921	40.0	37.0	41.0	33.0	41.0
24-25	38.102536932653734	40.0	37.0	41.0	33.0	41.0
26-27	37.95502711246496	40.0	37.0	41.0	33.0	41.0
28-29	37.88581355593962	40.0	37.0	41.0	33.0	41.0
30-31	37.76100741811768	40.0	37.0	41.0	32.0	41.0
32-33	37.647496013980245	40.0	36.0	41.0	32.0	41.0
34-35	37.553212474620636	40.0	36.0	41.0	32.0	41.0
36-37	37.47563235572826	40.0	36.0	41.0	32.0	41.0
38-39	37.354158220823194	40.0	36.0	41.0	31.0	41.0
40-41	37.22340432653105	39.0	36.0	41.0	31.0	41.0
42-43	37.10271442610729	39.0	35.0	41.0	31.0	41.0
44-45	36.90454931305745	39.0	35.0	41.0	30.5	41.0
46-47	36.67153011280509	39.0	35.0	41.0	30.0	41.0
48-49	36.415567608793964	39.0	35.0	41.0	30.0	41.0
50-51	36.208820054480135	38.5	35.0	41.0	29.0	41.0
52-53	36.01120792191878	38.0	35.0	40.0	29.0	41.0
54-55	35.80728697961577	38.0	34.5	40.0	29.0	41.0
56-57	35.537292347302284	37.5	34.0	40.0	28.0	41.0
58-59	35.24714389290733	37.0	34.0	40.0	28.0	41.0
60-61	34.98395718569225	36.5	34.0	40.0	28.0	41.0
62-63	34.703910273869155	36.0	33.5	39.0	27.0	41.0
64-65	34.43003421429495	35.0	33.0	39.0	27.0	41.0
66-67	34.20562810090581	35.0	33.0	39.0	26.5	41.0
68-69	34.01647185269696	35.0	33.0	38.0	26.0	40.5
70-71	33.818862753317774	35.0	33.0	37.5	26.0	40.0
72-73	33.624268009466874	35.0	33.0	37.0	26.0	40.0
74-75	33.44950923112388	35.0	33.0	36.5	26.0	39.0
76-77	33.30209302121261	35.0	33.0	36.0	26.5	39.0
78-79	33.19362015103992	35.0	33.0	36.0	27.0	39.0
80-81	33.05384756021285	35.0	33.0	35.5	27.0	38.0
82-83	32.867678264300196	35.0	33.0	35.0	26.0	37.0
84-85	32.72944014987685	35.0	33.0	35.0	26.0	37.0
86-87	32.60085926913279	35.0	33.0	35.0	26.0	36.0
88-89	32.494435784186365	35.0	33.0	35.0	26.0	36.0
90-91	32.40362241442138	35.0	33.0	35.0	26.0	36.0
92-93	32.32296853146707	35.0	33.0	35.0	26.0	36.0
94-95	32.2841422307235	35.0	33.0	35.0	26.0	35.0
96-97	32.2356834847205	35.0	32.5	35.0	26.0	35.0
98-99	32.19680154077287	35.0	32.0	35.0	25.0	35.0
100	31.67775639907306	34.0	31.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5128095535797002
1101	2	0.6210844128421229
1101	3	0.5176329325442204
1101	4	0.3646310510733173
1101	5	0.3515998170622723
1101	6	0.3874812956373006
1101	7	0.42231232725837486
1101	8	0.41955364267811035
1101	9	0.5023963730144487
1101	10-11	0.5337628831550632
1101	12-13	0.494365166005295
1101	14-15	0.6734357019054329
1101	16-17	0.6841503774431246
1101	18-19	0.6675168711927171
1101	20-21	0.6312338515512437
1101	22-23	0.6962326238269227
1101	24-25	0.713780139781079
1101	26-27	0.7283024770544699
1101	28-29	0.7658821619603131
1101	30-31	0.7857719291994627
1101	32-33	0.7386803812997087
1101	34-35	0.7437546227100356
1101	36-37	0.7363507653339738
1101	38-39	0.7783775046646184
1101	40-41	0.7618655312995273
1101	42-43	0.6885959668690234
1101	44-45	0.7252946567845129
1101	46-47	0.70382996480717
1101	48-49	0.6068028957199161
1101	50-51	0.5458040883163378
1101	52-53	0.6623334460000763
1101	54-55	0.6199189990714018
1101	56-57	0.6141978286747758
1101	58-59	0.5665716142318189
1101	60-61	0.5883380002851268
1101	62-63	0.6047904340809751
1101	64-65	0.5570511109659861
1101	66-67	0.5649101046895595
1101	68-69	0.5304810010006094
1101	70-71	0.5243759838475555
1101	72-73	0.4729027838277915
1101	74-75	0.44641462119541586
1101	76-77	0.505928324377372
1101	78-79	0.46306887645340566
1101	80-81	0.46787643717032523
1101	82-83	0.44559763054503776
1101	84-85	0.4481471150039624
1101	86-87	0.46486539755547085
1101	88-89	0.5278869501241914
1101	90-91	0.48209174172620806
1101	92-93	0.5415791130870744
1101	94-95	0.5079494032999818
1101	96-97	0.4832261983750925
1101	98-99	0.4876577802285951
1101	100	0.5931123490302959
1102	1	0.4117870509904833
1102	2	0.4967793747549507
1102	3	0.4218104604426749
1102	4	0.3068223073894174
1102	5	0.298014243109499
1102	6	0.34322145103473645
1102	7	0.31762439992662905
1102	8	0.3475755758652852
1102	9	0.43886500434231834
1102	10-11	0.4774433723924645
1102	12-13	0.5247354118345413
1102	14-15	0.5999043332333045
1102	16-17	0.6190955668925895
1102	18-19	0.5762276015068721
1102	20-21	0.6160586147113847
1102	22-23	0.6083655052254073
1102	24-25	0.6118717910918292
1102	26-27	0.6287855272559995
1102	28-29	0.6628456350437304
1102	30-31	0.6645831791490195
1102	32-33	0.6758710771807301
1102	34-35	0.6678055371159104
1102	36-37	0.6547402990968294
1102	38-39	0.7174244511901406
1102	40-41	0.6301954347701866
1102	42-43	0.6967427649913347
1102	44-45	0.7159978010494754
1102	46-47	0.709133877540701
1102	48-49	0.6873688006432275
1102	50-51	0.6221076827227492
1102	52-53	0.6396462718930707
1102	54-55	0.6505922385206446
1102	56-57	0.717761612911751
1102	58-59	0.6186316390703084
1102	60-61	0.6579324246286333
1102	62-63	0.5241511794147939
1102	64-65	0.5664613987606018
1102	66-67	0.6001647439711277
1102	68-69	0.5523213352889229
1102	70-71	0.5541439188908868
1102	72-73	0.611073972084661
1102	74-75	0.5626481387998012
1102	76-77	0.5270867660988543
1102	78-79	0.47189187312217484
1102	80-81	0.46639522967932834
1102	82-83	0.47081160465742045
1102	84-85	0.47796490635174393
1102	86-87	0.4775523923232541
1102	88-89	0.5302035645583061
1102	90-91	0.5198757365971147
1102	92-93	0.5219638853935109
1102	94-95	0.49002377527643404
1102	96-97	0.4634970006042778
1102	98-99	0.5111704976213574
1102	100	0.5564676962534527
1103	1	0.2717059082558251
1103	2	-0.18678478159025502
1103	3	0.2040950111721962
1103	4	0.21929287401066233
1103	5	0.20994161270699863
1103	6	0.2640187932274287
1103	7	0.31513806405694567
1103	8	0.33798113643909744
1103	9	0.41172046310497024
1103	10-11	0.40007790857752923
1103	12-13	0.3845036191925004
1103	14-15	0.49530093748641235
1103	16-17	0.49618698260366756
1103	18-19	0.4744194964193795
1103	20-21	0.48344689035172195
1103	22-23	0.48030451038486177
1103	24-25	0.4517952413421398
1103	26-27	0.49651092799393837
1103	28-29	0.5183769668562732
1103	30-31	0.5435675170702439
1103	32-33	0.5091089784927973
1103	34-35	0.49115351696801923
1103	36-37	0.5078085661635896
1103	38-39	0.5184189855380197
1103	40-41	0.5094268752833742
1103	42-43	0.5579452084593512
1103	44-45	0.5947025792848493
1103	46-47	0.5572732593249228
1103	48-49	0.5449204334493842
1103	50-51	0.5904320087022015
1103	52-53	0.5581964196340934
1103	54-55	0.5449079603082367
1103	56-57	0.49369375921362746
1103	58-59	0.4710614812247229
1103	60-61	0.5246452252777729
1103	62-63	0.511307968612094
1103	64-65	0.5288378091897457
1103	66-67	0.5506116414097733
1103	68-69	0.4567906158743753
1103	70-71	0.4343261072951279
1103	72-73	0.41421894200060194
1103	74-75	0.4247517259210767
1103	76-77	0.38405202782244174
1103	78-79	0.338536391394598
1103	80-81	0.3587577080540214
1103	82-83	0.3668570445888335
1103	84-85	0.37732831863338845
1103	86-87	0.4454039470486677
1103	88-89	0.4018143243911254
1103	90-91	0.36149185491240843
1103	92-93	0.41014613503755726
1103	94-95	0.4091719296619729
1103	96-97	0.42140290565196636
1103	98-99	0.3873574014901848
1103	100	0.46936510427892486
1104	1	0.3530709076503271
1104	2	0.3797073601570986
1104	3	0.33105472006710457
1104	4	0.24360915131249072
1104	5	0.28962509732308916
1104	6	0.29987824383414363
1104	7	0.28843985094191993
1104	8	0.30612851474021596
1104	9	0.3641058638015835
1104	10-11	0.34115923007720284
1104	12-13	0.3498431125853756
1104	14-15	0.46798781906046827
1104	16-17	0.40618408891143787
1104	18-19	0.43398812358111627
1104	20-21	0.49394312855181965
1104	22-23	0.4617171176204309
1104	24-25	0.4807841883640691
1104	26-27	0.5152424564775089
1104	28-29	0.5174040550403092
1104	30-31	0.5463283087240569
1104	32-33	0.5411649819864905
1104	34-35	0.5199327870851747
1104	36-37	0.5916132551457025
1104	38-39	0.48801660622659426
1104	40-41	0.5629565108428238
1104	42-43	0.538391533470211
1104	44-45	0.5959337449848334
1104	46-47	0.5688588259928835
1104	48-49	0.5701468515729928
1104	50-51	0.5843665073112803
1104	52-53	0.6097723498094894
1104	54-55	0.5698545290616437
1104	56-57	0.5927316537171947
1104	58-59	0.5878230005040592
1104	60-61	0.6258719054975117
1104	62-63	0.5838136299228225
1104	64-65	0.5286963321682663
1104	66-67	0.541737311701759
1104	68-69	0.5201758206850045
1104	70-71	0.48066264490232413
1104	72-73	0.4972540752322345
1104	74-75	0.44623089836969143
1104	76-77	0.3745711805658303
1104	78-79	0.3762755412494343
1104	80-81	0.43346782424426067
1104	82-83	0.446675582469787
1104	84-85	0.4348535994175933
1104	86-87	0.4700423727428671
1104	88-89	0.47510872695774964
1104	90-91	0.4677135933850636
1104	92-93	0.4415413663974235
1104	94-95	0.4434322936942934
1104	96-97	0.4426548537912396
1104	98-99	0.36644681297801895
1104	100	0.4864402040830065
1105	1	0.1358107130732904
1105	2	-0.39913071144087553
1105	3	0.0380113718215469
1105	4	0.10022893326505056
1105	5	0.12610117409128208
1105	6	0.21776983308222952
1105	7	0.2661319334877774
1105	8	0.23977862587152288
1105	9	0.31570829536624245
1105	10-11	0.34088716043619627
1105	12-13	0.3251478098935934
1105	14-15	0.408730154696606
1105	16-17	0.37227617714006556
1105	18-19	0.3842511035443721
1105	20-21	0.41776252562066674
1105	22-23	0.4096165055085663
1105	24-25	0.392099002662917
1105	26-27	0.423548642617007
1105	28-29	0.44702863893582645
1105	30-31	0.41039155743281697
1105	32-33	0.41183624710135547
1105	34-35	0.4835445243281171
1105	36-37	0.4284568500677324
1105	38-39	0.49058137121497225
1105	40-41	0.4788864400481643
1105	42-43	0.49949140240781986
1105	44-45	0.4623973444806637
1105	46-47	0.41139164193642586
1105	48-49	0.47907972892358686
1105	50-51	0.5485901771079753
1105	52-53	0.5096862392508683
1105	54-55	0.5388525900223442
1105	56-57	0.5068778198731962
1105	58-59	0.4618051703908179
1105	60-61	0.40440863151972195
1105	62-63	0.464015677321008
1105	64-65	0.5047001001776223
1105	66-67	0.4200117336244631
1105	68-69	0.398497070310178
1105	70-71	0.4280454453010023
1105	72-73	0.4316252226364625
1105	74-75	0.39980778311230125
1105	76-77	0.3647790183232189
1105	78-79	0.37488026192322366
1105	80-81	0.3440199814772029
1105	82-83	0.31875726487947986
1105	84-85	0.3625138336085172
1105	86-87	0.3471542400550973
1105	88-89	0.34511961112269773
1105	90-91	0.32144933369448836
1105	92-93	0.3801902140773237
1105	94-95	0.33101832494237016
1105	96-97	0.3270692909581143
1105	98-99	0.3546540595615326
1105	100	0.34790710151806437
1106	1	0.2810366934982014
1106	2	0.3248811713552797
1106	3	0.2612376126537548
1106	4	0.18272205902635363
1106	5	0.21775104277404722
1106	6	0.23357958841928905
1106	7	0.26426010184795246
1106	8	0.2438789443565028
1106	9	0.3167335977042498
1106	10-11	0.2838730727440435
1106	12-13	0.2632706073532489
1106	14-15	0.36243312076999956
1106	16-17	0.40098527649059434
1106	18-19	0.426571546716481
1106	20-21	0.3719306270972851
1106	22-23	0.3271086376104506
1106	24-25	0.31156429396789065
1106	26-27	0.36048202974500754
1106	28-29	0.3555540826133168
1106	30-31	0.36014898460871336
1106	32-33	0.3794186908525248
1106	34-35	0.33104285823537083
1106	36-37	0.347649587445261
1106	38-39	0.3246287002413055
1106	40-41	0.3650961433037665
1106	42-43	0.3321315983300437
1106	44-45	0.4004408119408964
1106	46-47	0.3616234215633227
1106	48-49	0.32442091387958527
1106	50-51	0.323725160601235
1106	52-53	0.3869096550065336
1106	54-55	0.3335614135467182
1106	56-57	0.3347859868970602
1106	58-59	0.35981690071165673
1106	60-61	0.346460846609304
1106	62-63	0.3185315082001807
1106	64-65	0.300330594214806
1106	66-67	0.3578232501372014
1106	68-69	0.3404005534291059
1106	70-71	0.34321787947533977
1106	72-73	0.3671760697311157
1106	74-75	0.3597361625394413
1106	76-77	0.3982383750829328
1106	78-79	0.32129182366459474
1106	80-81	0.3490741102177708
1106	82-83	0.33951807392383415
1106	84-85	0.32690253604349095
1106	86-87	0.32243729593472636
1106	88-89	0.28137646180481113
1106	90-91	0.28865961772689275
1106	92-93	0.2812775002049861
1106	94-95	0.29559205361509555
1106	96-97	0.3087749844553045
1106	98-99	0.3681153094137599
1106	100	0.35266032277346326
1107	1	0.06796003508383208
1107	2	-0.5132150571635208
1107	3	-0.0051652391748149284
1107	4	0.0618714773557727
1107	5	0.11383267584946566
1107	6	0.1868576649871656
1107	7	0.17267139223312
1107	8	0.1660208683577551
1107	9	0.13118370751931252
1107	10-11	0.2653315823117879
1107	12-13	0.2156153723179841
1107	14-15	0.20729897565889388
1107	16-17	0.2951391572694675
1107	18-19	0.2785256080628784
1107	20-21	0.20412441898933054
1107	22-23	0.21761875378935258
1107	24-25	0.21895154921223536
1107	26-27	0.2721896345349606
1107	28-29	0.1971839761095282
1107	30-31	0.3067050342673667
1107	32-33	0.31767116977552945
1107	34-35	0.29934653244810505
1107	36-37	0.32320835079057986
1107	38-39	0.3622333349801181
1107	40-41	0.3721095358259987
1107	42-43	0.3745970175804061
1107	44-45	0.3989800000079171
1107	46-47	0.412622984372625
1107	48-49	0.4687751263025248
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2216	90-91	-0.9220897480056962
2216	92-93	-0.9246740849013371
2216	94-95	-0.939855674777391
2216	96-97	-0.8925445370791252
2216	98-99	-0.8813244602260362
2216	100	-0.8756363242467557
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	9.0
13	66.0
14	283.0
15	813.0
16	2081.0
17	4135.0
18	7367.0
19	11300.0
20	16176.0
21	23138.0
22	31572.0
23	37741.0
24	40573.0
25	44009.0
26	50630.0
27	62461.0
28	78093.0
29	97431.0
30	120820.0
31	148983.0
32	184750.0
33	232421.0
34	316054.0
35	428958.0
36	635076.0
37	1044221.0
38	842808.0
39	317954.0
40	531.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.727611382922774	21.97494404650176	13.72721639243978	24.570228178135686
2	14.988445210928505	20.303125200694772	40.11127825651653	24.597151331860196
3	17.920985300033408	26.82773712641603	29.74030568048246	25.510971893068103
4	12.416240791299588	17.307355912399963	38.88486216033893	31.391541135961514
5	12.740185129749602	38.74041565810511	35.071374884082104	13.448024328063187
6	29.499872187978642	39.03052803727174	17.79760432996169	13.671995444787926
7	25.915252010696943	32.381299430679064	24.129200038824944	17.574248519799045
8	26.14887527513054	35.01020195136115	19.71005706984561	19.130865703662703
9	26.15284978970634	14.453636032363427	21.734005402829602	37.65950877510063
10-11	25.67410904980234	25.924106016620165	27.322026466920114	21.079758466657378
12-13	26.010604004888233	23.77411327533889	27.819081351619445	22.396201368153427
14-15	23.333195607775043	24.666668410028166	25.56508186389751	26.435054118299284
16-17	21.844418051635422	27.068332446890576	26.989499183374416	24.097750318099592
18-19	21.3087950987123	27.169666588431486	29.452261687060112	22.069276625796107
20-21	22.90063393547267	26.352679818134465	28.521803886868508	22.224882359524358
22-23	23.375396512613513	25.94445518267402	28.23687544295197	22.443272861760494
24-25	22.778510215810936	26.047193181824124	29.08626423307811	22.088032369286832
26-27	22.666765402131034	26.284572970685506	28.067030502669155	22.98163112451431
28-29	21.82368300275831	26.915444085064657	28.61944399469674	22.64142891748029
30-31	22.772389172752906	26.434559199139496	28.365566646543073	22.427484981564525
32-33	23.26232130896245	26.683416107829	27.54746511735905	22.506797465849505
34-35	23.060285565793688	26.473444544167958	28.442688167060794	22.023581722977564
36-37	22.863269586303236	26.028039572777335	28.1454726722837	22.963218168635724
38-39	23.095736851992005	25.953506965232204	28.800347163388945	22.150409019386846
40-41	22.49270072038386	25.36037758050388	29.306376246943927	22.840545452168342
42-43	22.754197405313565	25.519485878186316	28.36575044031141	23.36056627618871
44-45	22.8159833221001	26.514561993850798	28.11998348275416	22.54947120129494
46-47	22.955101791501985	26.23082349013421	27.417467112983733	23.39660760538007
48-49	23.814984561546563	25.948117466752752	27.41529612324907	22.82160184845161
50-51	23.146129513113596	26.083818747074023	27.093920708015844	23.676131031796537
52-53	22.486579737757346	26.678615312591386	26.98593942254068	23.848865527110583
54-55	22.656217559867127	26.572216514356782	26.803293545856288	23.9682723799198
56-57	23.74726032734349	25.995708565245657	26.62498999833175	23.63204110907911
58-59	22.54765293065445	26.378201554623192	27.65140778558341	23.422737729138944
60-61	22.716671798673598	27.074404617467458	26.787957048448934	23.420966535410013
62-63	23.209031435900073	26.232193651737973	26.380265238184293	24.17850967417766
64-65	22.264676313441097	26.375171583977934	26.899179953050524	24.460972149530445
66-67	22.98134404695115	25.944240070721257	26.851726340865245	24.222689541462344
68-69	22.478792274902112	25.922662208176423	27.17189017429008	24.426655342631385
70-71	22.420854234565162	25.623543538015486	27.364447056953157	24.591155170466195
72-73	23.074588250749194	25.690634663038566	27.640908046885883	23.593869039326357
74-75	23.143694242946637	26.182237275201555	27.12177245275496	23.552296029096848
76-77	22.893904289471802	26.15000395893957	26.629689369154224	24.326402382434402
78-79	22.5793053575405	26.222711531942572	26.88108319951519	24.316899911001737
80-81	22.2138348018234	26.63227487272261	27.47229414345146	23.681596182002536
82-83	23.1313403892799	25.450629815924096	28.46419677158543	22.95383302321057
84-85	23.53987591901526	26.075384747178692	26.80894429911649	23.575795034689563
86-87	22.03788430319158	26.71112198959013	27.37728238857927	23.873711318639018
88-89	22.978511576832673	25.49316324990421	27.17530889353339	24.353016279729726
90-91	22.52685706097119	25.454505516804588	27.528052102776734	24.49058531944749
92-93	22.99281991503395	25.807793554304194	27.03026572862455	24.169120802037313
94-95	21.685518066593172	25.7278829881369	27.81841154554982	24.768187399720105
96-97	22.129786060771895	25.667100954848255	27.304969880148178	24.898143104231675
98-99	21.50742235049711	26.429751405699864	27.20498606903791	24.857840174765116
100	22.360930601799435	25.581374900347694	26.41694692132995	25.64074757652292
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	112.0
1	90.5
2	90.0
3	119.5
4	86.5
5	69.5
6	152.0
7	373.0
8	613.0
9	688.0
10	600.0
11	481.0
12	477.0
13	572.0
14	731.5
15	1032.5
16	1376.5
17	1837.5
18	2476.0
19	3218.5
20	4157.5
21	5107.0
22	6108.5
23	7126.0
24	8107.5
25	9323.5
26	11217.0
27	13461.0
28	17254.0
29	25883.0
30	38787.0
31	50690.0
32	66815.5
33	82440.0
34	93184.5
35	105973.5
36	117779.5
37	141819.5
38	152520.0
39	139945.0
40	129144.5
41	118659.0
42	115148.5
43	122350.5
44	135984.5
45	165837.5
46	195865.0
47	215048.5
48	225777.5
49	219264.5
50	209671.0
51	215760.0
52	220338.0
53	214801.0
54	207975.5
55	180668.0
56	157266.0
57	132021.5
58	104920.0
59	100534.5
60	83572.0
61	58072.0
62	42930.0
63	34423.0
64	24778.5
65	12340.5
66	6789.0
67	4766.0
68	3500.5
69	2429.0
70	1765.5
71	1371.5
72	1080.0
73	893.5
74	680.5
75	498.0
76	343.0
77	190.5
78	87.0
79	40.0
80	17.0
81	6.5
82	4.5
83	2.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012467424669351766
2	0.003953596078032691
3	0.0022382792611084544
4	0.026817514137766715
5	0.002823997198594779
6	0.0
7	5.438809419515871E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008796228311178553
16-17	0.002416086492131089
18-19	0.0
20-21	6.27554933021062E-5
22-23	6.27554933021062E-4
24-25	1.0459248883684367E-5
26-27	1.568887332552655E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.8699220869632163E-4
36-37	0.0
38-39	0.0
40-41	4.288292042310591E-4
42-43	7.216881729742213E-4
44-45	0.0
46-47	0.0
48-49	5.12503195300534E-4
50-51	0.0
52-53	0.0
54-55	3.660737109289528E-4
56-57	1.1505173772052804E-4
58-59	1.4642948437158115E-4
60-61	4.183699553473747E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001370161603762652
70-71	7.949029151600119E-4
72-73	0.0
74-75	1.882664799063186E-4
76-77	0.002876293443013201
78-79	5.752586886026402E-4
80-81	0.0031586931628726786
82-83	0.0019454202923652921
84-85	0.005721209139375349
86-87	0.007907192156065381
88-89	0.007792140418344852
90-91	0.006076823601420617
92-93	0.009015872537735925
94-95	0.0018408278035284486
96-97	0.004643906504355859
98-99	0.0020709312789695044
100	0.08053621640436963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4780458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.64583285129174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.42072617407966	12.65598818574804
2	15.855002503413443	6.229694580777453
3	6.908155755143433	4.071494198287109
4	3.6258928848957885	2.8493474221340263
5	2.161593915196446	2.123315637515933
6	1.3769498430008704	1.6230795876124502
7	0.9587820124758735	1.3185249810548285
8	0.672535765085522	1.057002018190862
9	0.5112199961733577	0.903900833555381
>10	2.7129775438781514	9.898260696663531
>50	0.3018125940159581	4.193229207431728
>100	0.35594715431675283	15.626193440480082
>500	0.0741068424774193	10.172758288247907
>1k	0.06035175948519619	21.131529937800778
>5k	0.0031988565098160526	3.9934347085154727
>10k+	7.463998522904122E-4	2.1522462759844556
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19259	0.40286934850175443	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15927	0.33316891394088183	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	14360	0.300389627939415	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14059	0.294093160111437	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13907	0.290913548450797	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	13800	0.2886752691896885	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	11430	0.2390984294810246	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8003	0.16741073763225198	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7993	0.16720155265457828	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7767	0.16247397215915294	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7537	0.15766271767265813	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7489	0.15665862977982445	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7284	0.15237033773751385	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7222	0.151073390875937	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7090	0.1483121491706443	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6555	0.13712075286510206	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6532	0.13663962741645258	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6517	0.13632584994994204	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6515	0.13628401295440729	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6435	0.1346105331330178	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6422	0.134338592662042	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6393	0.1337319562267883	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6273	0.13122173649470406	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6249	0.13071969254828722	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6158	0.12881610925145667	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6048	0.1265150744970461	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5998	0.12546914960867767	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5889	0.12318903335203446	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5777	0.12084616160208918	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5625	0.11766654994144912	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5503	0.11511449321383015	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5423	0.11344101339244064	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5398	0.11291805094825642	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5238	0.10957109130547743	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5166	0.10806495946622688	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5116	0.10701903457785843	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5020	0.10501085879219105	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4996	0.10450881484577419	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	4969	0.10394401540605523	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4950	0.10354656394847522	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4939	0.10331646047303418	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	4859	0.10164298065164469	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0918497767368734E-5
2	0.0	0.0	0.0	0.0	2.0918497767368734E-5
3	0.0	0.0	0.0	0.0	2.0918497767368734E-5
4	0.0	0.0	0.0	0.0	2.0918497767368734E-5
5	0.0	0.0	0.0	2.0918497767368734E-5	2.0918497767368734E-5
6	0.0	0.0	0.0	2.0918497767368734E-5	4.183699553473747E-5
7	0.0	0.0	0.0	2.0918497767368734E-5	4.183699553473747E-5
8	0.0	0.0	0.0	2.0918497767368734E-5	4.183699553473747E-5
9	0.0	0.0	0.0	2.0918497767368734E-5	4.183699553473747E-5
10-11	0.0	0.0	0.0	2.0918497767368734E-5	4.183699553473747E-5
12-13	0.0	0.0	0.0	2.0918497767368734E-5	5.229624441842184E-5
14-15	0.0	0.0	0.0	2.0918497767368734E-5	6.27554933021062E-5
16-17	0.0	0.0	0.0	2.0918497767368734E-5	8.367399106947494E-5
18-19	0.0	0.0	0.0	4.183699553473747E-5	8.367399106947494E-5
20-21	0.0	0.0	0.0	6.27554933021062E-5	1.0459248883684368E-4
22-23	0.0	0.0	0.0	1.882664799063186E-4	1.255109866042124E-4
24-25	0.0	0.0	0.0	2.823997198594779E-4	1.255109866042124E-4
26-27	0.0	0.0	0.0	6.484734307884307E-4	1.4642948437158112E-4
28-29	0.0	0.0	0.0	0.0017989908079937109	1.6734798213894987E-4
30-31	0.0	0.0	0.0	0.006704378534441679	1.7780723102263422E-4
32-33	0.0	0.0	0.0	0.018868484986166598	1.882664799063186E-4
34-35	0.0	0.0	0.0	0.03709895579042845	2.3010347544105607E-4
36-37	0.0	0.0	0.0	0.07059992996486947	2.3010347544105607E-4
38-39	0.0	0.0	0.0	0.12458011345356448	2.4056272432474042E-4
40-41	0.0	0.0	0.0	0.18508686824567855	2.510219732084248E-4
42-43	0.0	0.0	0.0	0.25080232898186744	2.614812220921092E-4
44-45	0.0	0.0	0.0	0.3297591988048007	3.033182176268467E-4
46-47	0.0	0.0	0.0	0.4279506273248295	3.4515521316158407E-4
48-49	0.0	0.0	0.0	0.5463597839370202	3.5561446204526845E-4
50-51	0.0	0.0	0.0	0.6746320122465254	3.5561446204526845E-4
52-53	1.0459248883684367E-5	0.0	0.0	0.8006659612949218	3.765329598126372E-4
54-55	2.0918497767368734E-5	0.0	0.0	0.940537496616433	3.765329598126372E-4
56-57	2.0918497767368734E-5	0.0	0.0	1.1121005560555077	3.765329598126372E-4
58-59	2.0918497767368734E-5	0.0	0.0	1.3225615620930045	3.765329598126372E-4
60-61	2.0918497767368734E-5	0.0	0.0	1.5421534924059577	3.765329598126372E-4
62-63	2.0918497767368734E-5	0.0	0.0	1.7752483130277477	3.765329598126372E-4
64-65	2.0918497767368734E-5	0.0	0.0	2.0206013733412154	3.765329598126372E-4
66-67	2.0918497767368734E-5	0.0	0.0	2.3011079691527465	3.765329598126372E-4
68-69	2.0918497767368734E-5	0.0	0.0	2.619613016158703	3.765329598126372E-4
70-71	2.0918497767368734E-5	0.0	0.0	2.975530796421598	3.765329598126372E-4
72-73	2.0918497767368734E-5	0.0	0.0	3.3477440864452737	3.869922086963216E-4
74-75	2.0918497767368734E-5	0.0	0.0	3.7105963487180516	3.9745145758000595E-4
76-77	2.0918497767368734E-5	0.0	0.0	4.139362797455808	3.9745145758000595E-4
78-79	2.0918497767368734E-5	0.0	0.0	4.6006993472173585	3.9745145758000595E-4
80-81	2.0918497767368734E-5	0.0	0.0	5.102011146212351	3.9745145758000595E-4
82-83	2.0918497767368734E-5	0.0	0.0	5.631019036251338	4.28829204231059E-4
84-85	2.0918497767368734E-5	0.0	0.0	6.146900568941302	4.497477019984278E-4
86-87	2.0918497767368734E-5	0.0	0.0	6.698469058822397	4.7066619976579647E-4
88	2.0918497767368734E-5	0.0	0.0	7.142955758632332	4.8112544864948085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9805	0.0	41.912746	1
GGGCACC	4575	0.0	40.297848	94
GTGGTAT	2305	0.0	38.35034	1
GTATCAA	15990	0.0	36.34564	1
GTACTAG	4755	0.0	31.544415	1
CAACGCA	18190	0.0	30.17783	5
ATCAACG	18335	0.0	29.974836	3
TAGGCAT	6050	0.0	28.975523	5
CAGTACG	605	0.0	28.746386	9
TATTCTC	2665	0.0	28.745392	5
TATCAAC	20640	0.0	27.995203	2
TAGTACT	7385	0.0	27.373898	4
CTGTGCG	7365	0.0	26.996317	9
AACGCAG	21395	0.0	26.886442	6
AGGGCTA	6165	0.0	26.529192	5
CTAGGCA	7020	0.0	26.185349	4
GTACTGT	7970	0.0	25.355629	6
ACTGTGC	8365	0.0	25.281961	8
CTATTGA	6430	0.0	25.146896	9
GCTATTG	6520	0.0	25.083946	8
>>END_MODULE