FastQCFastQC Report
Mon 16 Jan 2017
ERR1146452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4345534
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC179570.41322884598302534No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT143300.3297638449037564No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN132620.3051868884238393No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG83130.1912998494546355No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT72760.167436269052319No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT64740.1489805395608457No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT63560.14626510803965634No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG59790.13758953445077174No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC59760.13752049805616526No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG59310.13648495213706763No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC58750.13519627277107946No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57350.13197457435610904No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA51660.1188806715124079No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA51510.11853548953937537No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC51380.11823633182941383No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT49610.11416318454762983No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG48990.11273643239242863No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA48980.11271342026089312No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA48210.11094148613265942No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT47490.1092846126621032No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA46740.10755870279694048No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC46650.10735159361312097No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA45670.10509640472264167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC47550.038.53239494
GTACTAG55350.032.7348021
TAGGCAT64900.030.9870645
CAACGCA68150.030.6814255
GCATCGT58100.028.4433578
GTATCAA85650.027.8201921
CTAGGCA76050.027.7426954
CTGTGCG72300.027.663739
CTAACGC28500.027.0418073
TAGTACT74200.026.7878594
TAACGCC29100.026.4820984
ATCAACG78750.026.375973
CGCCTAC30200.025.649647
ACATGGG154500.025.3673023
GTACATG163600.025.2360211
ACTGTGC82500.025.1525888
TCTAACG31750.025.0135122
TACTGTG85650.024.5558477
GTACTGT82200.024.5007696
ACGCAGA83950.024.4938877
Produced by FastQC (version 0.11.5)