##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146451_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1647067 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.193558003408484 31.0 30.0 34.0 26.0 34.0 2 30.24558138800668 31.0 30.0 34.0 26.0 34.0 3 30.40657423164935 31.0 30.0 34.0 26.0 34.0 4 33.85014756533887 35.0 35.0 37.0 28.0 37.0 5 34.06268536738335 37.0 35.0 37.0 28.0 37.0 6 34.24128101649781 37.0 35.0 37.0 30.0 37.0 7 34.07489737818801 37.0 35.0 37.0 29.0 37.0 8 34.04033229977894 37.0 35.0 37.0 29.0 37.0 9 35.35582766214125 38.0 35.0 39.0 28.0 39.0 10-11 35.293498685845805 38.5 35.0 39.0 28.0 39.0 12-13 35.27689310756636 38.0 35.0 39.0 27.5 39.0 14-15 36.232773773015914 39.0 35.5 41.0 27.0 41.0 16-17 36.055103708592306 39.0 35.0 41.0 27.0 41.0 18-19 35.99856107857179 39.0 35.0 41.0 27.0 41.0 20-21 36.32886124243883 39.0 35.0 41.0 27.0 41.0 22-23 36.01243118828803 39.0 34.5 41.0 27.0 41.0 24-25 36.10248490194995 39.0 35.0 41.0 27.0 41.0 26-27 36.16427473806469 39.0 35.0 41.0 27.0 41.0 28-29 35.99092568790462 39.0 35.0 41.0 27.0 41.0 30-31 35.929122494713326 39.0 34.5 41.0 27.0 41.0 32-33 36.03548671669094 39.0 35.0 41.0 27.0 41.0 34-35 36.085960983979405 39.0 35.0 41.0 27.5 41.0 36-37 36.0055128297756 39.0 35.0 41.0 27.0 41.0 38-39 35.826994590991134 39.0 34.0 41.0 27.0 41.0 40-41 35.784310838599765 39.0 34.0 41.0 27.0 41.0 42-43 35.61278351153899 38.0 34.0 40.5 27.0 41.0 44-45 35.40029610210149 38.0 33.5 40.0 26.0 41.0 46-47 35.25600172913427 38.0 33.0 40.0 26.0 41.0 48-49 35.091524206361974 38.0 33.0 40.0 26.0 41.0 50-51 34.84386852508125 37.5 33.0 40.0 25.5 41.0 52-53 34.631078152862024 37.0 33.0 40.0 25.0 41.0 54-55 34.404513902591695 37.0 33.0 40.0 25.0 41.0 56-57 34.2195262852088 36.0 32.5 40.0 25.0 41.0 58-59 33.91540113425866 36.0 32.0 39.0 24.5 41.0 60-61 33.65001605884885 35.0 32.0 39.0 24.5 41.0 62-63 33.343165760712836 35.0 32.0 38.5 24.0 40.5 64-65 33.03926828720385 35.0 31.0 38.0 23.5 40.0 66-67 32.74615756371781 35.0 31.0 37.0 23.0 40.0 68-69 32.43722052594096 35.0 31.0 37.0 23.0 40.0 70-71 32.41519592099168 35.0 31.0 36.0 23.0 39.5 72-73 32.231085620682094 35.0 31.0 36.0 23.0 39.0 74-75 31.989458534473705 35.0 31.0 35.5 23.0 39.0 76-77 31.790925020050793 34.5 30.0 35.0 23.0 38.5 78-79 31.60175086987961 34.0 30.0 35.0 23.0 37.0 80-81 31.43935006894073 34.0 30.0 35.0 22.5 37.0 82-83 31.231405886949346 34.0 30.0 35.0 21.0 36.5 84-85 31.08913480750935 34.0 30.0 35.0 21.0 36.0 86-87 30.97927103147595 34.0 30.0 35.0 20.0 36.0 88-89 31.044964776782003 34.0 30.0 35.0 21.0 36.0 90-91 30.9922106993826 34.0 30.0 35.0 20.5 35.5 92-93 30.870529553442573 34.0 30.0 35.0 20.0 35.0 94-95 30.88009200597183 34.0 30.0 35.0 20.0 35.0 96-97 30.809394517648645 34.0 30.0 35.0 20.0 35.0 98-99 30.751546840535326 34.0 30.0 35.0 20.0 35.0 100 30.24640466963396 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9141464780464617 1101 2 0.9756027717832971 1101 3 1.0402027190532834 1101 4 1.0861122394907525 1101 5 1.0643751096352077 1101 6 0.816565175707467 1101 7 0.8677292688964755 1101 8 0.850623340499169 1101 9 1.1306635051733949 1101 10-11 1.0542584558565125 1101 12-13 1.0153506016556264 1101 14-15 1.3029555156275379 1101 16-17 1.3860774469661905 1101 18-19 1.5577629812171878 1101 20-21 1.1977698256012346 1101 22-23 1.5238396933669094 1101 24-25 1.2839268724778563 1101 26-27 1.2557252557087466 1101 28-29 1.4429645805925801 1101 30-31 1.5070178855699439 1101 32-33 1.4045181881084403 1101 34-35 1.1695117560491326 1101 36-37 1.1648694185639243 1101 38-39 1.2754270139711181 1101 40-41 1.120949837763753 1101 42-43 1.1579557304633923 1101 44-45 1.2572120603239583 1101 46-47 1.401154154028248 1101 48-49 1.258332204169868 1101 50-51 1.393280209249518 1101 52-53 1.275511772992047 1101 54-55 1.2123906828707192 1101 56-57 1.1007342089949574 1101 58-59 1.0704659660802136 1101 60-61 1.0313575595330278 1101 62-63 1.117468720564851 1101 64-65 1.1012790303380697 1101 66-67 1.1057928023664516 1101 68-69 1.1266084191395365 1101 70-71 1.033145726972485 1101 72-73 0.9809779997105039 1101 74-75 0.9955754047853489 1101 76-77 1.033966221917293 1101 78-79 0.9611699914414835 1101 80-81 1.0506718083040738 1101 82-83 1.0919616070545821 1101 84-85 1.0317452334770998 1101 86-87 1.0170488876892563 1101 88-89 1.021585339806773 1101 90-91 0.9979751421624528 1101 92-93 1.0983304532295506 1101 94-95 0.9998096121543192 1101 96-97 1.09828410596673 1101 98-99 1.0281509799735602 1101 100 1.1407718848283643 1102 1 1.022459289881521 1102 2 1.1206823126520327 1102 3 1.0969331972615173 1102 4 1.1868384024520893 1102 5 0.9930067269585621 1102 6 0.8202739492785298 1102 7 0.8657969233465437 1102 8 0.8581904996817968 1102 9 0.9557905321012683 1102 10-11 1.0033278804291186 1102 12-13 0.9484336071035742 1102 14-15 1.2110372599778216 1102 16-17 1.417849942655529 1102 18-19 1.4777277048763011 1102 20-21 1.2248785528556212 1102 22-23 1.4952712455717645 1102 24-25 1.425464161989197 1102 26-27 1.2464040243271342 1102 28-29 1.4305361765460631 1102 30-31 1.416415757295674 1102 32-33 1.2559158855734083 1102 34-35 1.221919535416653 1102 36-37 1.1783238975242227 1102 38-39 1.3191630272516193 1102 40-41 1.20963395114331 1102 42-43 1.262748933376301 1102 44-45 1.2996617539918702 1102 46-47 1.2017261764620315 1102 48-49 1.107467091175522 1102 50-51 1.19389293074412 1102 52-53 1.107457612286936 1102 54-55 1.0092986433777256 1102 56-57 1.0342706363740746 1102 58-59 1.0023987931401663 1102 60-61 0.9636804670280767 1102 62-63 1.0075491556664744 1102 64-65 0.910137324233439 1102 66-67 0.8638729990059701 1102 68-69 1.0603878944311944 1102 70-71 0.8290178677201538 1102 72-73 0.8728600847864456 1102 74-75 0.8264518905876841 1102 76-77 0.7783786994468294 1102 78-79 0.7950421574111068 1102 80-81 0.8627917593276209 1102 82-83 0.8134029041049935 1102 84-85 0.8727815806045527 1102 86-87 0.8407697907268954 1102 88-89 0.8920809282578723 1102 90-91 0.9117541818021913 1102 92-93 0.8860255221084117 1102 94-95 0.9809590682772935 1102 96-97 1.041223449462219 1102 98-99 1.0522754915848296 1102 100 0.9478067461534785 1103 1 0.7468014150212525 1103 2 0.9785510712011032 1103 3 0.909578594403424 1103 4 0.8615840703799691 1103 5 0.9606662854192507 1103 6 0.7655966265230845 1103 7 0.8063547803755782 1103 8 0.8880381225884761 1103 9 0.9544795511996469 1103 10-11 1.0430011955994658 1103 12-13 0.9536296203709611 1103 14-15 1.1835348000373784 1103 16-17 1.3580003766772961 1103 18-19 1.3892256827193634 1103 20-21 1.0247450703559977 1103 22-23 1.290237061137546 1103 24-25 1.2202221933022699 1103 26-27 1.0160067580155498 1103 28-29 1.2539299287416483 1103 30-31 1.360275947373097 1103 32-33 1.237951285612155 1103 34-35 1.203745670203027 1103 36-37 1.1880449571617149 1103 38-39 1.260939969153192 1103 40-41 1.1716890366295658 1103 42-43 1.1804397853598658 1103 44-45 1.2795669581654465 1103 46-47 1.1615255513616987 1103 48-49 1.130851187253306 1103 50-51 1.1673950328750493 1103 52-53 1.2134333570668545 1103 54-55 1.1986951004710917 1103 56-57 1.1005610698863748 1103 58-59 1.2073672952393295 1103 60-61 1.1103885898373065 1103 62-63 1.0644516804154591 1103 64-65 1.1129353472766113 1103 66-67 1.0811594196015761 1103 68-69 1.0510624838536557 1103 70-71 1.016640013167354 1103 72-73 1.0678440574080952 1103 74-75 1.0222023545848842 1103 76-77 1.035343396561668 1103 78-79 0.9900685606730981 1103 80-81 1.0674145961054649 1103 82-83 1.0594676658506579 1103 84-85 0.9863205685130829 1103 86-87 1.0830352699872776 1103 88-89 1.1166080302555024 1103 90-91 1.1319258118911328 1103 92-93 1.226046827769565 1103 94-95 1.1520214159935023 1103 96-97 1.0454417067521398 1103 98-99 1.0269686136446765 1103 100 1.1897013830982601 1104 1 0.5307700079589779 1104 2 0.6681322553864177 1104 3 0.6952081377499155 1104 4 0.8529011951720378 1104 5 0.7388526392334072 1104 6 0.49843611577766467 1104 7 0.4621459650303734 1104 8 0.594751585168872 1104 9 0.5997441930040779 1104 10-11 0.6885811515189388 1104 12-13 0.5838162837893179 1104 14-15 0.713498523053282 1104 16-17 0.8684108179521033 1104 18-19 0.9234642276811229 1104 20-21 0.7845645355781841 1104 22-23 0.9429791519556261 1104 24-25 0.9096371555597429 1104 26-27 0.903463727472591 1104 28-29 0.9686569377747318 1104 30-31 1.0523472883018812 1104 32-33 0.7478046930982316 1104 34-35 0.6657113015989253 1104 36-37 0.7594582115205952 1104 38-39 0.8595076690604344 1104 40-41 0.8374294284311503 1104 42-43 0.7623618183936145 1104 44-45 0.8045814550015251 1104 46-47 0.731055168683298 1104 48-49 0.6528419676462178 1104 50-51 0.7725760815450826 1104 52-53 0.7774408535489172 1104 54-55 0.7596649229435357 1104 56-57 0.6031159389834286 1104 58-59 0.5974939664993073 1104 60-61 0.5928337778393811 1104 62-63 0.5402924017424979 1104 64-65 0.6010117789105465 1104 66-67 0.8367897115811829 1104 68-69 0.7470064150403104 1104 70-71 0.5917374306368899 1104 72-73 0.5591148684569092 1104 74-75 0.5358579300616881 1104 76-77 0.5086565294897234 1104 78-79 0.53835285853771 1104 80-81 0.6312438163977347 1104 82-83 0.46095643064408875 1104 84-85 0.5441239267035698 1104 86-87 0.44662670410185257 1104 88-89 0.39188613476722267 1104 90-91 0.48398004845665454 1104 92-93 0.32590814031373583 1104 94-95 0.23323224757262295 1104 96-97 0.31550221914709553 1104 98-99 0.3613449578534933 1104 100 0.4597980582790413 1105 1 0.5555877935535172 1105 2 0.5793834656025076 1105 3 0.6143268678651843 1105 4 0.7228415996632265 1105 5 0.6998640441898871 1105 6 0.40975929170647873 1105 7 0.46892434530158766 1105 8 0.43526298639542205 1105 9 0.357503947173889 1105 10-11 0.43564251501467766 1105 12-13 0.5481687554678132 1105 14-15 0.717301077221741 1105 16-17 0.8399094702771066 1105 18-19 0.8004436687444993 1105 20-21 0.6562068121579259 1105 22-23 0.9485347733334777 1105 24-25 0.7980415923336892 1105 26-27 0.5568892880847613 1105 28-29 0.900013934663086 1105 30-31 0.8240941345727393 1105 32-33 0.7339060954203518 1105 34-35 0.6731002202541205 1105 36-37 0.6271616817745524 1105 38-39 0.7775992056439591 1105 40-41 0.5766327796930142 1105 42-43 0.5655939565256602 1105 44-45 0.6266042502873006 1105 46-47 0.5835373056085871 1105 48-49 0.7125998381281207 1105 50-51 0.7370099534657086 1105 52-53 0.7004995601641539 1105 54-55 0.6640496241446812 1105 56-57 0.5778281821795659 1105 58-59 0.6222269876009108 1105 60-61 0.6135953740096269 1105 62-63 0.6270698136945683 1105 64-65 0.5220231195074447 1105 66-67 0.7051241982674554 1105 68-69 0.7389463283508846 1105 70-71 0.6410272153526364 1105 72-73 0.6042805205094126 1105 74-75 0.6174707050873636 1105 76-77 0.5076965478380551 1105 78-79 0.5588660852359943 1105 80-81 0.6873680766099568 1105 82-83 0.6988208832241689 1105 84-85 0.5863414711544657 1105 86-87 0.6494420261871774 1105 88-89 0.5840191560736123 1105 90-91 0.5903093890093807 1105 92-93 0.6038801670071656 1105 94-95 0.6217207906165285 1105 96-97 0.47728406679070545 1105 98-99 0.6743547789792039 1105 100 0.8468137176963353 1106 1 0.5989747474643323 1106 2 0.48964804298485376 1106 3 0.5749098801526848 1106 4 0.7759896447661419 1106 5 0.621798103662563 1106 6 0.28148176805382263 1106 7 0.30040731670469967 1106 8 0.3996042232892876 1106 9 0.5811205750615045 1106 10-11 0.5315869617480828 1106 12-13 0.36698805537434964 1106 14-15 0.729531908457659 1106 16-17 0.7381730684866525 1106 18-19 0.6547533043753972 1106 20-21 0.57582688163874 1106 22-23 0.9239270197276497 1106 24-25 0.7221183469251855 1106 26-27 0.5694933459471088 1106 28-29 0.7099849865841534 1106 30-31 0.6143784252029363 1106 32-33 0.633886490835323 1106 34-35 0.6122184002845827 1106 36-37 0.6254574864386342 1106 38-39 0.6272905956694004 1106 40-41 0.4541930154973812 1106 42-43 0.4614211681424365 1106 44-45 0.5880818443896914 1106 46-47 0.6115327737650276 1106 48-49 0.619811820136583 1106 50-51 0.5846391645086939 1106 52-53 0.5472772772215464 1106 54-55 0.49353236946873835 1106 56-57 0.5905934986955117 1106 58-59 0.5346942510377843 1106 60-61 0.5189682376720413 1106 62-63 0.4411218339100742 1106 64-65 0.4771028878322596 1106 66-67 0.6322508316621622 1106 68-69 0.48558626999894017 1106 70-71 0.45904014653088865 1106 72-73 0.5271321771874042 1106 74-75 0.4300392513341862 1106 76-77 0.4301882589017829 1106 78-79 0.4320752803447796 1106 80-81 0.5324874027313982 1106 82-83 0.5790615442201421 1106 84-85 0.447113828417919 1106 86-87 0.5370034678470859 1106 88-89 0.505267809673267 1106 90-91 0.5571436077183698 1106 92-93 0.6478934413788409 1106 94-95 0.6800781618273106 1106 96-97 0.5390039488130647 1106 98-99 0.4732221501130134 1106 100 0.45298220454081317 1107 1 0.6739050320228017 1107 2 0.8025627106788242 1107 3 0.6816311768041565 1107 4 0.7595603223433898 1107 5 0.6868608024845599 1107 6 0.4610157010067084 1107 7 0.5580356194741753 1107 8 0.5280305199497022 1107 9 0.6796385653684425 1107 10-11 0.70864725416412 1107 12-13 0.6146226989767314 1107 14-15 0.8253561802631637 1107 16-17 0.8922541383821141 1107 18-19 1.022606189894809 1107 20-21 0.7674598725230553 1107 22-23 1.0494022897222948 1107 24-25 0.7285083842613673 1107 26-27 0.6161906143239975 1107 28-29 0.6967430785095772 1107 30-31 0.7476266864975543 1107 32-33 0.7795560130101364 1107 34-35 0.6952302532515731 1107 36-37 0.6456229041387616 1107 38-39 0.7338927006277416 1107 40-41 0.4351128477977042 1107 42-43 0.6674290075425091 1107 44-45 0.5188480556642503 1107 46-47 0.555275877403858 1107 48-49 0.5304406223825495 1107 50-51 0.6089225450989133 1107 52-53 0.6299287335967705 1107 54-55 0.5209311126157345 1107 56-57 0.5078957282270622 1107 58-59 0.6528411587909844 1107 60-61 0.6455238716335288 1107 62-63 0.6811417661745125 1107 64-65 0.740299686117659 1107 66-67 0.7235152642140434 1107 68-69 0.6100368492829631 1107 70-71 0.6471550555811518 1107 72-73 0.6196375934588829 1107 74-75 0.5962215813170744 1107 76-77 0.5129637930037525 1107 78-79 0.5008873561974987 1107 80-81 0.5984972350826396 1107 82-83 0.6644185556852555 1107 84-85 0.548713254315853 1107 86-87 0.6676722474824288 1107 88-89 0.7662703659634751 1107 90-91 0.7638429929177057 1107 92-93 0.6993029794945116 1107 94-95 0.7003315281324234 1107 96-97 0.7028217137981478 1107 98-99 0.7774520651084167 1107 100 0.8210476669309479 1108 1 0.3574968681372255 1108 2 0.40392845094530117 1108 3 0.3874000101760018 1108 4 0.5629030047524708 1108 5 0.29065786327191034 1108 6 0.09865247211428141 1108 7 0.12340481751017762 1108 8 0.30980271231317147 1108 9 0.37766011713410563 1108 10-11 0.42956248575828937 1108 12-13 0.21223521793686473 1108 14-15 0.38016211220219986 1108 16-17 0.5488203794851501 1108 18-19 0.6290541078179572 1108 20-21 0.30384080735554875 1108 22-23 0.5340640344088143 1108 24-25 0.3065965269911004 1108 26-27 0.43085910657285353 1108 28-29 0.4831290675688322 1108 30-31 0.6660114397474857 1108 32-33 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-1.3230439401612877 2214 92-93 -1.3503997142644195 2214 94-95 -1.4955407475125142 2214 96-97 -1.2838737372942504 2214 98-99 -1.5843686947838584 2214 100 -1.2990899624429346 2215 1 -4.727598996030743 2215 2 -2.660199126638794 2215 3 -4.430865275452209 2215 4 -2.580541219230014 2215 5 -2.1699668705000406 2215 6 -1.890240438550073 2215 7 -1.6684318856541012 2215 8 -1.708651719376853 2215 9 -2.329562967072313 2215 10-11 -1.9633895611905459 2215 12-13 -1.8373576876512203 2215 14-15 -4.322538681681561 2215 16-17 -4.06302023019639 2215 18-19 -2.949401228771542 2215 20-21 -2.4548887137760502 2215 22-23 -3.8572139485040395 2215 24-25 -6.150875404572012 2215 26-27 -4.248477072411848 2215 28-29 -2.503854725902208 2215 30-31 -4.648925029687877 2215 32-33 -4.444890316047655 2215 34-35 -3.482674599262218 2215 36-37 -2.4736566187000975 2215 38-39 -2.306933334014225 2215 40-41 -2.7073325569248965 2215 42-43 -2.4439457734813814 2215 44-45 -4.0808277043985015 2215 46-47 -3.6468182061436245 2215 48-49 -2.790171775824497 2215 50-51 -5.857515569490971 2215 52-53 -6.474145921076705 2215 54-55 -3.925201992883018 2215 56-57 -2.719696061855988 2215 58-59 -2.333890895007414 2215 60-61 -3.750622179477965 2215 62-63 -3.4035495240110833 2215 64-65 -2.4968670143223974 2215 66-67 -2.1959119031844097 2215 68-69 -2.2951708232526897 2215 70-71 -1.9487670917792812 2215 72-73 -1.8606785408643027 2215 74-75 -2.141958935873987 2215 76-77 -2.268873266318824 2215 78-79 -2.1532372359465164 2215 80-81 -2.2106672132512983 2215 82-83 -2.1004451448473596 2215 84-85 -1.9133493354155426 2215 86-87 -2.0631167399523314 2215 88-89 -2.0845757266908294 2215 90-91 -2.324152349736675 2215 92-93 -4.4037839187766465 2215 94-95 -2.755484572827367 2215 96-97 -2.284515186087372 2215 98-99 -3.8254224297897537 2215 100 -3.910910807479329 2216 1 -0.84293066101527 2216 2 -0.6569414434218714 2216 3 -0.8754835220462098 2216 4 -0.8198948675505378 2216 5 -0.8517752006797537 2216 6 -0.7697934612587716 2216 7 -0.9410529987815863 2216 8 -0.788313603553263 2216 9 -1.098521203061047 2216 10-11 -1.14186457828081 2216 12-13 -1.3381826890461497 2216 14-15 -1.801041009031941 2216 16-17 -1.4523558867680606 2216 18-19 -3.4253259723986034 2216 20-21 -1.7807422495532563 2216 22-23 -1.268181456696169 2216 24-25 -1.3725709485016822 2216 26-27 -1.2196351413126507 2216 28-29 -1.2804021396642256 2216 30-31 -1.4696866917762605 2216 32-33 -1.337046293122981 2216 34-35 -1.6711013498802672 2216 36-37 -1.6632541614978749 2216 38-39 -1.5455261287363484 2216 40-41 -1.5988660346730157 2216 42-43 -1.6840163398532866 2216 44-45 -1.5465113851611108 2216 46-47 -1.8745366208503995 2216 48-49 -1.8709640276816444 2216 50-51 -1.7002821083301853 2216 52-53 -1.7696177888517681 2216 54-55 -1.4829643317496348 2216 56-57 -1.5539200310407537 2216 58-59 -1.6531839900854592 2216 60-61 -1.6337967058945218 2216 62-63 -1.6314521263998891 2216 64-65 -1.5194770249466387 2216 66-67 -1.5775522913962448 2216 68-69 -1.6749295743975772 2216 70-71 -1.8371625466232153 2216 72-73 -1.8006533789860093 2216 74-75 -1.864677618179556 2216 76-77 -1.7115405089238926 2216 78-79 -1.86969031069464 2216 80-81 -1.77318986900044 2216 82-83 -1.985908305223301 2216 84-85 -1.7157860786660741 2216 86-87 -1.5849506191745562 2216 88-89 -1.5399039982948786 2216 90-91 -1.5045821665434715 2216 92-93 -1.4202078423367652 2216 94-95 -1.6229330202039662 2216 96-97 -1.6649779498097779 2216 98-99 -1.5473422063276772 2216 100 -1.5904037438200405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 6643.0 1 998.0 2 176.0 3 160.0 4 159.0 5 79.0 6 96.0 7 125.0 8 280.0 9 510.0 10 985.0 11 1926.0 12 3427.0 13 4719.0 14 5738.0 15 6611.0 16 8069.0 17 8891.0 18 9997.0 19 11066.0 20 12636.0 21 14167.0 22 15891.0 23 18111.0 24 20124.0 25 22727.0 26 25684.0 27 29144.0 28 34071.0 29 40012.0 30 46581.0 31 55853.0 32 67652.0 33 84312.0 34 115057.0 35 152774.0 36 226274.0 37 325430.0 38 213380.0 39 56509.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.20627017680476 22.35809172264309 12.862998537612782 23.57263956293937 2 15.513681422145902 19.952399188237248 40.54209799693638 23.991821392680475 3 18.670332432142263 27.14803527333418 28.295528856007884 25.88610343851567 4 12.315110794766653 16.372594404728577 39.95058439352437 31.361710406980396 5 13.07984693504251 39.20551824048466 34.53522006136256 13.179414763110273 6 29.81059104135276 38.652237831770684 17.76527929433725 13.771891832539302 7 27.036345133564033 31.553801437893824 23.682723798460582 17.72712963008156 8 26.780886649855614 34.614988032344094 19.482294397623917 19.121830920176375 9 26.29686355870149 14.287466438857702 21.597144251891628 37.818525750549185 10-11 26.51261145252931 25.46109765102924 26.597654080642503 21.42863681579895 12-13 26.40996746938946 23.038025204891582 26.901237359335852 23.650769966383102 14-15 23.8791578839448 24.149321935221653 24.337496963934594 27.634023216898957 16-17 22.743603655176244 26.81191880885147 25.740058454013997 24.70441908195829 18-19 22.031417350946093 27.702285813674603 27.423020471931576 22.843276363447732 20-21 24.049909846517853 27.11952224909824 25.998927739596343 22.83164016478757 22-23 24.816198618656706 26.344625721730974 25.939275921843652 22.899899737768663 24-25 24.055321811973332 26.374585676829298 26.590859091828627 22.979233419368736 26-27 23.890736775374865 26.82912542988402 25.254193285435058 24.025944509306058 28-29 22.7747363719459 27.307241244229232 26.362134212642673 23.5558881711822 30-31 23.747340300571206 26.847877091529053 26.338911150936685 23.06587145696306 32-33 24.065445725051678 27.089068733266718 25.146078214506375 23.69940732717523 34-35 24.036033331014274 26.99658975926923 26.186108334340297 22.781268575376206 36-37 23.59620447909555 26.453649042803224 25.605299885643618 24.344846592457607 38-39 23.975449106912897 26.575429332415407 26.28580007983038 23.16332148084132 40-41 23.387217368217065 25.44731276645742 27.28273706889975 23.88273279642577 42-43 23.39700073820377 25.765081527419586 26.508686787678375 24.329230946698267 44-45 23.92271820007447 26.560254899926143 26.248283270971562 23.268743629027828 46-47 23.433498138314107 26.56918757248367 25.609961545504483 24.387352743697736 48-49 24.450337211941918 26.128311401742323 25.943442857038246 23.477908529277514 50-51 23.882194762163707 25.80291376080942 26.096261155775917 24.21863032125095 52-53 23.45496271112588 26.388305320526257 25.96734275159272 24.18938921675514 54-55 23.227023939143894 26.34779748158058 25.91029046291459 24.51488811636094 56-57 24.6988249581214 25.610115551378716 25.458467716098166 24.232591774401723 58-59 23.188391645563517 26.29535295379501 26.68410454549477 23.832150855146704 60-61 23.293103332537967 26.997713376958384 25.75984294494102 23.94934034556263 62-63 23.91116242343324 26.118005865388955 25.184529090791486 24.786302620386316 64-65 22.71311135251254 26.37957865957109 25.983748123375115 24.92356186454125 66-67 23.90807045787488 25.91844562314307 25.713090460932325 24.460393458049722 68-69 22.858785681564385 26.16515294725884 26.223231673689728 24.75282969748705 70-71 22.85930678071817 25.602267359470947 26.263977228758456 25.274448631052426 72-73 23.66157091171861 25.848526202205935 26.669374447661504 23.82052843841395 74-75 23.998834328121376 26.18419446580492 26.112331709936893 23.704639496136803 76-77 23.399751876336044 26.201852811285047 25.501785257695282 24.896610054683624 78-79 23.47371858652326 26.35908877263876 25.548976639421817 24.61821600141616 80-81 22.959865082869253 26.64958131323287 26.510471669874114 23.880081934023767 82-83 23.950727282158628 25.460032282967227 27.691523737745133 22.897716697129013 84-85 23.952403156034254 26.1309956838888 26.157727254972805 23.758873905104146 86-87 22.274058957152096 26.819434194266638 26.85457474575903 24.05193210282223 88-89 23.72163747285141 25.114303393865434 26.64791485317968 24.516144280103475 90-91 23.246672454902555 25.482232785906167 26.575767424630936 24.69532733456034 92-93 23.495969866762994 25.676228520815425 26.742726102858246 24.085075509563335 94-95 22.27161245591479 25.68149263745178 27.547473604120583 24.499421302512843 96-97 22.69902839323512 25.4917955559402 27.014805041834734 24.79437100898995 98-99 21.898977276423835 26.298842983728367 26.882322649862484 24.91985708998531 100 22.864062385881915 25.26453914246417 25.94530433432371 25.926094137330203 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5482.0 1 3198.5 2 856.5 3 625.5 4 340.0 5 183.5 6 130.5 7 122.5 8 117.5 9 101.0 10 101.0 11 111.5 12 104.5 13 101.5 14 110.0 15 137.0 16 164.0 17 171.0 18 188.0 19 235.0 20 291.0 21 327.0 22 405.5 23 536.0 24 694.5 25 966.0 26 1430.5 27 1881.0 28 2540.0 29 4146.5 30 6895.5 31 9869.0 32 13629.0 33 18285.5 34 22508.5 35 27322.0 36 32871.0 37 41676.5 38 46720.0 39 45864.0 40 43234.5 41 39443.0 42 39362.5 43 43294.5 44 49423.5 45 60983.0 46 72772.5 47 81605.0 48 87583.0 49 86294.5 50 82220.5 51 85821.0 52 87763.0 53 83499.0 54 78190.0 55 66765.0 56 58112.5 57 47343.0 58 36585.5 59 34659.0 60 28559.0 61 19680.0 62 14507.0 63 11727.5 64 8682.0 65 4148.0 66 1989.5 67 1161.0 68 797.5 69 573.0 70 397.0 71 288.5 72 213.0 73 159.5 74 122.0 75 81.0 76 57.0 77 44.5 78 36.5 79 28.0 80 26.0 81 29.0 82 21.5 83 11.5 84 10.5 85 10.5 86 8.5 87 8.0 88 8.0 89 4.5 90 3.0 91 3.5 92 1.5 93 1.5 94 2.5 95 1.0 96 0.5 97 1.0 98 1.0 99 1.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9818665543053197 2 0.43586569338102216 3 0.4708369483451493 4 0.4555977382826564 5 0.423722896518478 6 0.47095837631377474 7 0.485894016454704 8 0.4897189974664054 9 0.5019225083132622 10-11 0.5146724450189336 12-13 0.5134278083405229 14-15 0.5120010297091739 16-17 0.5169188624385044 18-19 0.5149760149404973 20-21 0.5143992320895264 22-23 0.47754584361170493 24-25 0.5100278252190106 26-27 0.4922386278153833 28-29 0.4981582412858736 30-31 0.5011332265171969 32-33 0.5041992827249893 34-35 0.5144295890816828 36-37 0.5323098574617791 38-39 0.5217152671992092 40-41 0.5265116719599142 42-43 0.5239920416109363 44-45 0.5328866403127499 46-47 0.5322795004696227 48-49 0.5060510592465273 50-51 0.5200759896227658 52-53 0.512517098575832 54-55 0.5208652714188312 56-57 0.5101188961954796 58-59 0.5153706558385299 60-61 0.5103617521327305 62-63 0.5039867837798948 64-65 0.5152795848620608 66-67 0.5125778125601448 68-69 0.5326741413676553 70-71 0.5166152925169407 72-73 0.5208652714188312 74-75 0.5177081442345697 76-77 0.5080546207288471 78-79 0.5365294793715132 80-81 0.5119099587327048 82-83 0.5119403157248612 84-85 0.5194081357953259 86-87 0.8273191072372892 88-89 1.0970409825465508 90-91 1.0868106761898575 92-93 1.086932104158483 94-95 1.091485652981937 96-97 1.0842606888487234 98-99 1.0801017809233018 100 1.076094657958662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1647067.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.05770265784825 #Duplication Level Percentage of deduplicated Percentage of total 1 79.08920331894986 22.981505534881496 2 10.590338502107743 6.154618144804177 3 3.7071082752653814 3.2316014994933036 4 1.7309686810427045 2.011918929751467 5 0.9236112005441203 1.3419009818434646 6 0.6064060951399812 1.0572460801490646 7 0.4202037616159882 0.8547109172522712 8 0.289242381496396 0.6723775294056147 9 0.22487303274155035 0.5880864349055289 >10 1.675720276116036 10.081616415839004 >50 0.2955987866663095 6.117889706149474 >100 0.36738687141657816 22.601655295256716 >500 0.05945177836119125 11.537237957126454 >1k 0.019468364040812626 9.893466834346501 >5k 4.186744955013468E-4 0.8741677387954968 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7308 0.44369779735736314 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 7063 0.42882287120074647 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4950 0.3005342223479676 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 4395 0.2668379610544076 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3957 0.24024523592543595 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 3630 0.22039176305517627 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 3338 0.20266327963586178 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 3265 0.1982311587810332 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 3234 0.19634902526733883 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3126 0.189791914961565 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3049 0.18511693816948552 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 2977 0.18074553129896961 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 2866 0.17400627904025762 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 2800 0.16999915607561805 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 2752 0.16708488482860745 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2538 0.15409209218568523 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 2500 0.15178496078180184 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 2470 0.1499635412524202 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 2404 0.14595641828778064 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 2348 0.14255643516626829 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2313 0.14043144571532304 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 2312 0.14037073173101033 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 2308 0.14012787579375946 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 2271 0.13788145837418878 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 2266 0.13757788845262517 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2241 0.13606003884480716 No Hit GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA 2178 0.13223505783310577 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 2129 0.12926007260178246 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2103 0.1276815090096517 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 2035 0.12355295807638669 No Hit ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT 1982 0.12033511690781248 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 1979 0.12015297495487434 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 1960 0.11899940925293265 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 1941 0.11784584355099094 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 1846 0.11207801504128248 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1827 0.11092444933934079 No Hit TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG 1750 0.10624947254726128 No Hit TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC 1684 0.10224234958262171 No Hit ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT 1673 0.10157449575518178 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 9.10709764690811E-5 14-15 0.0 0.0 0.0 0.0 1.5178496078180182E-4 16-17 0.0 0.0 0.0 0.0 1.821419529381622E-4 18-19 0.0 0.0 0.0 0.0 1.821419529381622E-4 20-21 0.0 0.0 0.0 3.0356992156360367E-5 1.821419529381622E-4 22-23 0.0 0.0 0.0 1.821419529381622E-4 1.821419529381622E-4 24-25 0.0 0.0 0.0 2.4285593725088293E-4 1.821419529381622E-4 26-27 0.0 0.0 0.0 3.33926913719964E-4 1.821419529381622E-4 28-29 0.0 0.0 0.0 0.0011232087097853335 1.821419529381622E-4 30-31 0.0 0.0 0.0 0.0037642670273886855 1.821419529381622E-4 32-33 0.0 0.0 0.0 0.010655304246882488 1.821419529381622E-4 34-35 0.0 0.0 0.0 0.02000525783104148 1.821419529381622E-4 36-37 0.0 0.0 0.0 0.03843195206995223 1.821419529381622E-4 38-39 0.0 0.0 0.0 0.07188535742626134 1.821419529381622E-4 40-41 0.0 0.0 0.0 0.11104587730796622 1.821419529381622E-4 42-43 0.0 0.0 0.0 0.15266531355433627 1.821419529381622E-4 44-45 0.0 0.0 0.0 0.20275435061233088 1.821419529381622E-4 46-47 0.0 0.0 0.0 0.26829509667791296 2.428559372508829E-4 48-49 0.0 0.0 0.0 0.34880183987658064 3.0356992156360365E-4 50-51 0.0 0.0 0.0 0.43732282900452746 3.0356992156360365E-4 52-53 0.0 0.0 0.0 0.5266330999285396 3.642839058763244E-4 54-55 0.0 0.0 0.0 0.6266290320915906 3.642839058763244E-4 56-57 0.0 0.0 0.0 0.7536426872738025 3.642839058763244E-4 58-59 0.0 0.0 0.0 0.912986539102538 3.642839058763244E-4 60-61 0.0 0.0 0.0 1.076489298856695 3.642839058763244E-4 62-63 0.0 0.0 0.0 1.2625473037830277 3.642839058763244E-4 64-65 0.0 0.0 0.0 1.4526427886661564 3.642839058763244E-4 66-67 0.0 0.0 0.0 1.6682077899684713 3.9464089803268476E-4 68-69 0.0 0.0 0.0 1.9179547644388482 4.2499789018904513E-4 70-71 0.0 0.0 0.0 2.2032497767243227 4.2499789018904513E-4 72-73 0.0 0.0 0.0 2.5022054354801595 4.553548823454055E-4 74-75 0.0 0.0 0.0 2.7953325517419754 4.8571187450176587E-4 76-77 0.0 0.0 0.0 3.146502237006752 4.8571187450176587E-4 78-79 0.0 0.0 0.0 3.5173128961966937 5.464258588144866E-4 80-81 0.0 0.0 0.0 3.92203231562529 5.464258588144866E-4 82-83 0.0 0.0 0.0 4.352190894480916 5.464258588144866E-4 84-85 0.0 0.0 0.0 4.774820939281765 5.464258588144866E-4 86-87 0.0 0.0 0.0 5.2287490429958225 5.767828509708469E-4 88 0.0 0.0 0.0 5.602625758393557 6.071398431272073E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCCG 130 0.0 43.365852 5 GGGCACC 1755 0.0 39.535233 94 GCATCGT 2025 0.0 37.33122 8 TATTCTC 710 0.0 35.73102 5 GTACTAG 2295 0.0 35.562668 1 TAGGCAT 2790 0.0 31.488169 5 CAACGCA 3305 0.0 30.988108 5 TAACCAC 545 0.0 29.30842 5 TCTAACG 1230 0.0 29.03164 2 TACCTGG 625 0.0 28.567131 2 ATCAACG 3630 0.0 28.346573 3 TAACGCC 1265 0.0 28.22753 4 CTAACGC 1250 0.0 28.191248 3 CGCCTAC 1240 0.0 28.020855 7 AGGGCTA 1590 0.0 27.774147 5 CTAGGCA 3260 0.0 27.671545 4 GTATCAA 4170 0.0 27.604805 1 CTATTGA 1605 0.0 27.5003 9 GGGTAAA 225 3.6379788E-12 27.257862 1 GTACATG 7470 0.0 27.030502 1 >>END_MODULE