##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146451_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1647067
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.781169800621345	31.0	31.0	34.0	30.0	34.0
2	31.73002616165584	31.0	31.0	34.0	30.0	34.0
3	31.921729959983413	31.0	31.0	34.0	30.0	34.0
4	35.681377260305744	37.0	35.0	37.0	33.0	37.0
5	35.592507165768	37.0	35.0	37.0	33.0	37.0
6	35.63962364615404	37.0	35.0	37.0	33.0	37.0
7	35.55251425716136	37.0	35.0	37.0	33.0	37.0
8	35.53277856942067	37.0	35.0	37.0	33.0	37.0
9	37.10391319843091	39.0	37.0	39.0	33.0	39.0
10-11	37.04774062014478	39.0	37.0	39.0	33.0	39.0
12-13	36.97640867068553	39.0	37.0	39.0	33.0	39.0
14-15	38.16138414527157	40.0	37.0	41.0	33.0	41.0
16-17	38.157884894785695	40.0	37.0	41.0	33.0	41.0
18-19	38.19216370675874	40.0	37.0	41.0	33.0	41.0
20-21	38.19700261130846	40.0	37.0	41.0	33.0	41.0
22-23	38.16690304644559	40.0	37.0	41.0	33.0	41.0
24-25	38.09101360175391	40.0	37.0	41.0	33.0	41.0
26-27	37.93958442492017	40.0	37.0	41.0	33.0	41.0
28-29	37.87070532042716	40.0	37.0	41.0	33.0	41.0
30-31	37.75985919212758	40.0	37.0	41.0	32.0	41.0
32-33	37.652134976901365	40.0	36.0	41.0	32.0	41.0
34-35	37.5726193894966	40.0	36.0	41.0	32.0	41.0
36-37	37.49907137960994	40.0	36.0	41.0	32.0	41.0
38-39	37.37565927797716	40.0	36.0	41.0	31.0	41.0
40-41	37.244803338297714	39.0	35.5	41.0	31.0	41.0
42-43	37.123433958667135	39.0	35.0	41.0	31.0	41.0
44-45	36.91352962569222	39.0	35.0	41.0	31.0	41.0
46-47	36.67035736858306	39.0	35.0	41.0	30.0	41.0
48-49	36.39071907821601	39.0	35.0	41.0	30.0	41.0
50-51	36.15923699521635	38.0	35.0	40.5	29.0	41.0
52-53	35.93638753007619	38.0	35.0	40.0	29.0	41.0
54-55	35.702802314659934	37.5	34.0	40.0	29.0	41.0
56-57	35.4126917727087	37.0	34.0	40.0	28.0	41.0
58-59	35.0800638346831	36.5	34.0	40.0	28.0	41.0
60-61	34.80240937375346	36.0	34.0	39.0	28.0	41.0
62-63	34.48594258764216	35.0	33.0	39.0	27.0	41.0
64-65	34.191378978511494	35.0	33.0	38.5	27.0	41.0
66-67	33.95314671473595	35.0	33.0	37.5	26.5	40.0
68-69	33.74777893066888	35.0	33.0	37.0	26.0	40.0
70-71	33.57059093528071	35.0	33.0	37.0	26.0	40.0
72-73	33.38566737115127	35.0	33.0	36.0	26.0	39.0
74-75	33.22607762768607	35.0	33.0	36.0	26.0	39.0
76-77	33.110232613488094	35.0	33.0	35.5	27.0	39.0
78-79	33.02596949608	35.0	33.0	35.0	27.0	37.5
80-81	32.91844594057194	35.0	33.0	35.0	27.0	37.0
82-83	32.76165207608433	35.0	33.0	35.0	27.0	37.0
84-85	32.65992093824963	35.0	33.0	35.0	27.0	36.0
86-87	32.56897837185737	35.0	33.0	35.0	26.5	36.0
88-89	32.493444103973914	35.0	33.0	35.0	26.0	36.0
90-91	32.418697600036914	35.0	33.0	35.0	26.0	35.5
92-93	32.35211864483958	35.0	33.0	35.0	26.0	35.0
94-95	32.331837138379925	35.0	33.0	35.0	26.0	35.0
96-97	32.29226163841544	35.0	33.0	35.0	26.0	35.0
98-99	32.25922078458254	35.0	33.0	35.0	26.0	35.0
100	31.748945246307528	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.46267231066309833
1101	2	0.5463469958758154
1101	3	0.4754321824241572
1101	4	0.2607605537239692
1101	5	0.3275463609139564
1101	6	0.36371445516017786
1101	7	0.447424099910414
1101	8	0.4195481567430761
1101	9	0.4575065854980238
1101	10-11	0.5124162157628263
1101	12-13	0.4497651557737754
1101	14-15	0.6289032316388656
1101	16-17	0.6817946771369847
1101	18-19	0.7276071589343189
1101	20-21	0.5894668927921458
1101	22-23	0.6396325226768482
1101	24-25	0.6491506654528365
1101	26-27	0.7163954970042354
1101	28-29	0.7573599113993694
1101	30-31	0.7315584331176481
1101	32-33	0.6327571823724085
1101	34-35	0.6570874462869583
1101	36-37	0.6040070007473304
1101	38-39	0.5811607849815843
1101	40-41	0.6882726966084789
1101	42-43	0.7085790186105072
1101	44-45	0.7833708693677934
1101	46-47	0.8040217270777248
1101	48-49	0.7090100332566109
1101	50-51	0.7855698095749162
1101	52-53	0.7459139477192451
1101	54-55	0.7219092240085629
1101	56-57	0.6668832868260992
1101	58-59	0.8039489055245852
1101	60-61	0.7035928665402764
1101	62-63	0.6540015485176269
1101	64-65	0.6919756631475167
1101	66-67	0.6552332053772361
1101	68-69	0.7342472640952664
1101	70-71	0.7106261572280701
1101	72-73	0.58838665683394
1101	74-75	0.602147428117938
1101	76-77	0.67183831969745
1101	78-79	0.591392023121962
1101	80-81	0.5786688054528
1101	82-83	0.6349714870900627
1101	84-85	0.5480896498420762
1101	86-87	0.578335821239591
1101	88-89	0.6097879054119915
1101	90-91	0.6095847313986411
1101	92-93	0.6183651548596245
1101	94-95	0.5543017671958523
1101	96-97	0.6112557144819561
1101	98-99	0.6069429895564795
1101	100	0.6825103451476231
1102	1	0.48181691958525263
1102	2	0.5546048926400715
1102	3	0.44092683078427086
1102	4	0.24134595525745794
1102	5	0.27214175492672865
1102	6	0.342211593614941
1102	7	0.3774670001598821
1102	8	0.36564941600641276
1102	9	0.47641273231729997
1102	10-11	0.48812346225926717
1102	12-13	0.48012350240595225
1102	14-15	0.5947435354028983
1102	16-17	0.60394855168758
1102	18-19	0.6328416887053905
1102	20-21	0.647492002392994
1102	22-23	0.6243832724567255
1102	24-25	0.664244571370368
1102	26-27	0.7260944844574553
1102	28-29	0.7011691256667376
1102	30-31	0.6662244027412498
1102	32-33	0.6058453583207566
1102	34-35	0.6939338537636672
1102	36-37	0.6954079197435874
1102	38-39	0.7733652524773689
1102	40-41	0.6327574579629456
1102	42-43	0.8386279950577844
1102	44-45	0.8237234149507486
1102	46-47	0.7777619478386057
1102	48-49	0.6547553190442628
1102	50-51	0.7091601890048054
1102	52-53	0.6149695919337859
1102	54-55	0.6171091423811532
1102	56-57	0.6868490422923799
1102	58-59	0.6957513121575047
1102	60-61	0.5990839838269437
1102	62-63	0.668691903184147
1102	64-65	0.7350951716404381
1102	66-67	0.7015206485131884
1102	68-69	0.7005286654269796
1102	70-71	0.6638239569565911
1102	72-73	0.6681791718754013
1102	74-75	0.608811756021673
1102	76-77	0.4918713719206522
1102	78-79	0.6071457124058384
1102	80-81	0.5007825541687794
1102	82-83	0.5119969818989247
1102	84-85	0.5789489961807632
1102	86-87	0.5964514891169088
1102	88-89	0.5382421092237166
1102	90-91	0.6093502818792373
1102	92-93	0.551564811841061
1102	94-95	0.5778636572831743
1102	96-97	0.5362894775056546
1102	98-99	0.5062979381036925
1102	100	0.5167253003901244
1103	1	0.30946973061147176
1103	2	-0.16141564349365822
1103	3	0.28377469835209723
1103	4	0.28765311221795287
1103	5	0.28435126424783874
1103	6	0.3149728997144692
1103	7	0.38647784640901506
1103	8	0.33396594591756923
1103	9	0.5064927240710944
1103	10-11	0.39427784521852516
1103	12-13	0.4423871126447523
1103	14-15	0.5925813499450143
1103	16-17	0.6129344430923851
1103	18-19	0.5192384254114941
1103	20-21	0.4798355096016138
1103	22-23	0.5043121874349055
1103	24-25	0.4881130580078974
1103	26-27	0.5601472133770571
1103	28-29	0.6395924919315803
1103	30-31	0.6450978684996471
1103	32-33	0.7082587065114723
1103	34-35	0.7524890260365069
1103	36-37	0.6810575513597357
1103	38-39	0.6706717732004037
1103	40-41	0.6920808988109286
1103	42-43	0.7040168996452678
1103	44-45	0.7256932333624135
1103	46-47	0.8019914725739667
1103	48-49	0.8097027613282322
1103	50-51	0.74646375661451
1103	52-53	0.8331649020225314
1103	54-55	0.6619790632187588
1103	56-57	0.7832737461961159
1103	58-59	0.7560758347276462
1103	60-61	0.7514526354478335
1103	62-63	0.7523284711984601
1103	64-65	0.6361761681043987
1103	66-67	0.7738272729062103
1103	68-69	0.6494140354606728
1103	70-71	0.5840930072033572
1103	72-73	0.5549538596954804
1103	74-75	0.5627317844094719
1103	76-77	0.530218896192757
1103	78-79	0.5644876645648154
1103	80-81	0.5650337251952138
1103	82-83	0.5694217071290595
1103	84-85	0.58760891955977
1103	86-87	0.6027430718849445
1103	88-89	0.6063098904314899
1103	90-91	0.600349358327847
1103	92-93	0.6496846679589865
1103	94-95	0.5912106483953963
1103	96-97	0.6391577147428436
1103	98-99	0.6592728369366014
1103	100	0.691595964689558
1104	1	0.3276526331001044
1104	2	0.45490608095099105
1104	3	0.32324415856224675
1104	4	0.25625493203084204
1104	5	0.23981013193392187
1104	6	0.34404016630271883
1104	7	0.2833910671428512
1104	8	0.23308319328244664
1104	9	0.3805700972063377
1104	10-11	0.30810065771892425
1104	12-13	0.40407232384177405
1104	14-15	0.4873197280504158
1104	16-17	0.49007042658693223
1104	18-19	0.5012572732016949
1104	20-21	0.5159361208161357
1104	22-23	0.4642482617805186
1104	24-25	0.463916982114263
1104	26-27	0.4732613621621766
1104	28-29	0.4687842346352653
1104	30-31	0.5435280229337778
1104	32-33	0.6023911993455329
1104	34-35	0.6044994865981295
1104	36-37	0.6013033821711886
1104	38-39	0.539822759810022
1104	40-41	0.5476702062999266
1104	42-43	0.5312812293077656
1104	44-45	0.5066774448170079
1104	46-47	0.40707989375749065
1104	48-49	0.46497878489368105
1104	50-51	0.46416436173846165
1104	52-53	0.4725718069575464
1104	54-55	0.48128757999892713
1104	56-57	0.40550586560183177
1104	58-59	0.5027872417391634
1104	60-61	0.33331376273703484
1104	62-63	0.4557891863587926
1104	64-65	0.43335179108768784
1104	66-67	0.3248142631853952
1104	68-69	0.3941231661199893
1104	70-71	0.3502701439481726
1104	72-73	0.40874581328530013
1104	74-75	0.3715638786915605
1104	76-77	0.3797474476638172
1104	78-79	0.28943985989968013
1104	80-81	0.273306628610122
1104	82-83	0.37363130917346155
1104	84-85	0.30133288711223116
1104	86-87	0.27864710980891516
1104	88-89	0.2691407640609498
1104	90-91	0.30052052884743574
1104	92-93	0.37228184117110885
1104	94-95	0.36874987867366826
1104	96-97	0.3757758978991106
1104	98-99	0.3632315834456321
1104	100	0.4806311542523467
1105	1	0.11671747230573715
1105	2	-0.3985759698202358
1105	3	-0.0216986191884736
1105	4	0.06347619397887172
1105	5	0.11798402830241628
1105	6	0.17160593045791472
1105	7	0.20124747184993907
1105	8	0.24338175006273133
1105	9	0.27942930901502905
1105	10-11	0.29392546011672493
1105	12-13	0.3258801142471839
1105	14-15	0.365985828937859
1105	16-17	0.3582656625445111
1105	18-19	0.4471141910301668
1105	20-21	0.4105661036921049
1105	22-23	0.3474495190156688
1105	24-25	0.36145301945686725
1105	26-27	0.41324788175550253
1105	28-29	0.45101462759876654
1105	30-31	0.48441828268173026
1105	32-33	0.43830937433016004
1105	34-35	0.4250165602440745
1105	36-37	0.3534416195290291
1105	38-39	0.40730309247299346
1105	40-41	0.44139535198294766
1105	42-43	0.45721498077359257
1105	44-45	0.4947971050744826
1105	46-47	0.4912546920377423
1105	48-49	0.5168112849898492
1105	50-51	0.5606667257097442
1105	52-53	0.45224010353058475
1105	54-55	0.49047227722147824
1105	56-57	0.4475384493947132
1105	58-59	0.5094758111412432
1105	60-61	0.356789065991336
1105	62-63	0.3702120074469093
1105	64-65	0.40555079266582084
1105	66-67	0.37709398923006177
1105	68-69	0.45868379715982854
1105	70-71	0.4159849324194411
1105	72-73	0.3606334432130325
1105	74-75	0.35994909535443753
1105	76-77	0.27132649388116903
1105	78-79	0.20749424345265055
1105	80-81	0.3113179087967737
1105	82-83	0.3332022297012287
1105	84-85	0.2753449273270263
1105	86-87	0.29841142502517926
1105	88-89	0.3105641990517114
1105	90-91	0.31973040090497307
1105	92-93	0.24636597874322774
1105	94-95	0.23732264207951914
1105	96-97	0.27606886563491884
1105	98-99	0.3055794414198303
1105	100	0.21304478918052183
1106	1	0.3217770550012453
1106	2	0.37304132996586503
1106	3	0.35145450892002117
1106	4	0.17104031900692007
1106	5	0.2006756850045477
1106	6	0.1989767382800096
1106	7	0.20497732338482422
1106	8	0.19900475706197795
1106	9	0.31789504422997794
1106	10-11	0.2255926150115144
1106	12-13	0.24129183845308688
1106	14-15	0.4583212360710647
1106	16-17	0.4135965476439907
1106	18-19	0.4020692234848795
1106	20-21	0.3409083267011255
1106	22-23	0.3590537279495294
1106	24-25	0.3992011272521978
1106	26-27	0.37993057618914605
1106	28-29	0.4028294552753806
1106	30-31	0.43019296638612303
1106	32-33	0.42799629106923476
1106	34-35	0.37052818935489995
1106	36-37	0.37238185210672015
1106	38-39	0.2640220999105125
1106	40-41	0.33102553767112397
1106	42-43	0.32161682737759634
1106	44-45	0.3976246174760121
1106	46-47	0.36001595543024933
1106	48-49	0.4722626952748783
1106	50-51	0.42582679737287066
1106	52-53	0.381236869453204
1106	54-55	0.38417030111250483
1106	56-57	0.34996904063670087
1106	58-59	0.31690090811904525
1106	60-61	0.3874389255370829
1106	62-63	0.3628515346670227
1106	64-65	0.31500047430637323
1106	66-67	0.3049839789840618
1106	68-69	0.26264670027438797
1106	70-71	0.36916868533466385
1106	72-73	0.3683339400888954
1106	74-75	0.35938774620885283
1106	76-77	0.339799468756965
1106	78-79	0.3043596076444217
1106	80-81	0.37149862715163096
1106	82-83	0.44760049194115226
1106	84-85	0.44910788027556947
1106	86-87	0.37625679510585996
1106	88-89	0.3164927372454116
1106	90-91	0.343868942387239
1106	92-93	0.30948066888588954
1106	94-95	0.2739385435863255
1106	96-97	0.28864160747422574
1106	98-99	0.3388913893051395
1106	100	0.383953003652568
1107	1	0.15842071051412532
1107	2	-0.5461241299881365
1107	3	0.021999034656133176
1107	4	0.09460692534828041
1107	5	0.20854123567361427
1107	6	0.2489616879201435
1107	7	0.22773206362882092
1107	8	0.2558253365400631
1107	9	0.24159446810467955
1107	10-11	0.3221252928731104
1107	12-13	0.29465172978770227
1107	14-15	0.3399637471510033
1107	16-17	0.4346115158359112
1107	18-19	0.44591848480606444
1107	20-21	0.3611402908026804
1107	22-23	0.3666787618937093
1107	24-25	0.3769718076647237
1107	26-27	0.4831905653407702
1107	28-29	0.36525743961308876
1107	30-31	0.40697611747039986
1107	32-33	0.3885444599971706
1107	34-35	0.5050801958149265
1107	36-37	0.47960056074317237
1107	38-39	0.5261714977515979
1107	40-41	0.503433431464444
1107	42-43	0.563263189477837
1107	44-45	0.43853572098050364
1107	46-47	0.556205895238449
1107	48-49	0.494355137103895
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2216	78-79	-0.8405532621486387
2216	80-81	-0.7024309316748685
2216	82-83	-0.710048021190353
2216	84-85	-0.683738565699862
2216	86-87	-0.7273442990909871
2216	88-89	-0.8493956368026012
2216	90-91	-0.8623778370256758
2216	92-93	-0.7934640329223583
2216	94-95	-0.776561875971332
2216	96-97	-0.7869027258980097
2216	98-99	-0.8317860922480023
2216	100	-0.8094033573562776
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	16.0
14	61.0
15	225.0
16	617.0
17	1104.0
18	2136.0
19	3367.0
20	5196.0
21	7271.0
22	10203.0
23	12444.0
24	13711.0
25	15149.0
26	17620.0
27	22085.0
28	27852.0
29	34348.0
30	42579.0
31	52204.0
32	64908.0
33	81699.0
34	111159.0
35	153377.0
36	231566.0
37	386341.0
38	268967.0
39	80751.0
40	107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.730693093355676	22.58067062581217	14.02654813519389	23.66208814563826
2	15.592848837738805	20.52021699994839	39.625077033767354	24.26185712854545
3	18.354492632192493	26.76812201356381	29.488958913923852	25.38842644031985
4	12.720537105134506	17.040030851357795	38.731754124395344	31.507677919112353
5	12.924116006299885	39.338099129697746	34.50079962890419	13.236985235098183
6	29.74633090214302	38.80394665183626	17.40396717316296	14.045755272857752
7	26.26821196274824	32.19287908234233	23.716700575509257	17.82220837940018
8	26.755317178961146	34.82760567724325	19.117376524452254	19.299700619343355
9	26.304030133564694	14.180418890063368	21.4262079198964	38.08934305647554
10-11	26.553078897215478	25.573337332361103	26.449895480876005	21.423688289547417
12-13	26.690869284613193	23.199693758663127	27.189543594765723	22.919893361957953
14-15	23.98867955002365	24.190023747200094	24.748699247266583	27.072597455509673
16-17	22.424887296792704	26.723395212580257	26.143227941288078	24.70848954933896
18-19	21.79410430783933	26.95488404539706	28.645252439639673	22.605759207123935
20-21	23.686468127890365	26.363469124206844	27.589921964315963	22.360140783586825
22-23	24.26225118840937	25.534886671934657	27.59265100213745	22.610211137518522
24-25	23.580188298350947	25.584296206529544	28.265729323700857	22.56978617141865
26-27	23.448953137854303	26.027843467015572	27.002729096908745	23.52047429822138
28-29	22.356376516559436	26.554262819909574	28.15019061155375	22.939170051977243
30-31	23.454844277737337	26.05567958073351	27.869934859966232	22.619541281562924
32-33	23.862872609310976	26.35934057327358	26.88551831831978	22.892268499095668
34-35	23.552959955022903	26.41241775878907	27.724361066275343	22.310261219912686
36-37	23.40378381693034	25.625399573909256	27.162404443777938	23.808412165382464
38-39	23.66834500357302	25.598746134795974	28.109390814095605	22.623518047535406
40-41	22.862694752730096	24.796447480702696	29.026569519624857	23.31428824694235
42-43	23.164127886750318	25.082435212995836	27.887266393086797	23.866170507167052
44-45	23.680639585396342	25.784682711753682	27.511236640646676	23.023441062203297
46-47	23.43143903678478	25.80159155638477	26.64229809716302	24.124671309667427
48-49	24.59305384248062	25.641561951957264	26.828498996238437	22.936885209323673
50-51	23.860686905875717	25.509253721919023	26.7911080727135	23.838951299491765
52-53	23.21818116688635	26.098270440728882	26.486020301542073	24.19752809084269
54-55	23.221796951651918	26.048719654947977	26.28914863854052	24.44033475485958
56-57	24.59633438318218	25.24357758130004	25.995765188024134	24.164322847493644
58-59	22.81145021344079	26.17058506029831	27.15879982587166	23.85916490038924
60-61	23.18220371655486	26.612764660489614	26.317633266001362	23.88739835695417
62-63	23.94483648813315	25.738388298715233	25.584083707584455	24.73269150556717
64-65	22.771083386407476	26.056893860419766	26.382533315281044	24.789489437891717
66-67	23.60593102769954	25.72937227204479	26.299173014819676	24.365523685435992
68-69	22.958514567987585	25.501810525088704	26.81319655429511	24.7264783526286
70-71	22.781013535132878	25.06015311038483	26.888455520698564	25.270377833783737
72-73	23.454601421800085	25.256926403115354	27.25575219465875	24.032719980425814
74-75	23.752247184530507	25.785383783600004	26.674452251616664	23.78791678025283
76-77	23.262776503589063	25.7794164300015	25.886822696176576	25.070984370232864
78-79	23.207575694101664	25.97836626176265	26.0353163248145	24.77874171932119
80-81	22.507989485178733	26.57260117358228	26.818683692282097	24.10072564895688
82-83	23.87425771939949	25.14444892049989	27.99824290368969	22.98305045641093
84-85	23.9506060399068	25.76200125989511	26.385148417161897	23.902244283036193
86-87	22.110216216724943	26.523443085658183	27.124347006910714	24.241993690706163
88-89	23.321198608438205	24.984235787085822	26.94018221213879	24.754383392337182
90-91	22.86058904480968	25.144683717734768	27.00469260079741	24.990034636658148
92-93	23.31025479495723	25.485962368978317	26.71823791488619	24.485544921178263
94-95	21.956758660259194	25.339929023973383	27.660280443342128	25.0430318724253
96-97	22.416753062039213	25.13244676248837	27.22211681059149	25.228683364880926
98-99	21.56960663448552	26.030938710606755	27.09983209175543	25.29962256315229
100	22.579939528869616	24.920583031139714	26.239135612829212	26.260341827161454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	58.0
1	35.5
2	26.0
3	37.5
4	29.5
5	31.0
6	54.5
7	135.5
8	230.0
9	260.5
10	221.0
11	151.5
12	136.0
13	176.5
14	219.5
15	280.0
16	355.5
17	457.5
18	621.5
19	830.0
20	1082.5
21	1374.5
22	1652.0
23	1939.0
24	2105.0
25	2285.0
26	2743.5
27	3121.5
28	3644.0
29	5171.5
30	7755.5
31	10502.0
32	14051.5
33	18716.5
34	22789.0
35	27110.5
36	32221.5
37	41217.5
38	46273.5
39	44876.0
40	42160.5
41	38470.5
42	38340.0
43	41955.5
44	48141.5
45	61001.0
46	73948.0
47	83232.0
48	89477.0
49	87439.0
50	81876.5
51	84044.0
52	86117.0
53	81955.5
54	79125.5
55	68623.0
56	58733.0
57	47873.5
58	36440.5
59	35619.5
60	29463.5
61	19006.0
62	13280.5
63	10785.0
64	7683.0
65	3190.0
66	1394.5
67	831.0
68	548.0
69	394.5
70	271.0
71	191.0
72	145.0
73	127.5
74	94.0
75	55.0
76	40.0
77	24.5
78	8.5
79	2.0
80	3.0
81	2.5
82	2.5
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014996354125242022
2	0.003764267027388685
3	0.002246417419570667
4	0.028049860752476983
5	0.0032178411685741992
6	0.0
7	2.428559372508829E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.00825710186653002
16-17	0.0025499873411342707
18-19	0.0
20-21	0.0
22-23	7.589248039090091E-4
24-25	0.0
26-27	1.2142796862544145E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.946408980326848E-4
36-37	0.0
38-39	0.0
40-41	6.374968352835677E-4
42-43	9.714237490035316E-4
44-45	0.0
46-47	0.0
48-49	7.589248039090091E-4
50-51	0.0
52-53	0.0
54-55	6.67853827439928E-4
56-57	2.732129294072433E-4
58-59	3.642839058763244E-4
60-61	2.428559372508829E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0018821335136943425
70-71	0.0012142796862544148
72-73	0.0
74-75	3.642839058763244E-4
76-77	0.0030053422234796764
78-79	6.071398431272074E-4
80-81	0.0035517680822941627
82-83	0.0019124905058507029
84-85	0.006344611360679316
86-87	0.007983888937122777
88-89	0.008530314795937263
90-91	0.006830323235181082
92-93	0.009380310576315354
94-95	0.0019428474980070633
96-97	0.005130331674424902
98-99	0.002216060427414307
100	0.08032460124572953
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1647067.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.86415201606528
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02137805589318	15.026649801646633
2	13.635652077980618	5.689926355863281
3	4.983159596753597	3.1190819804094487
4	2.3704503919862874	1.978297492997737
5	1.298591127868352	1.354700134927948
6	0.8202734140067147	1.0268585522743767
7	0.5495990337337013	0.8026842451391774
8	0.4185180474009254	0.6985619329951778
9	0.3214858334122141	0.6036776369391486
>10	2.45283255759234	10.73051467412732
>50	0.4333778125624065	6.404577720639586
>100	0.56533748486482	25.348648364980352
>500	0.09184917491812296	13.228975580664729
>1k	0.03691406713481524	13.012718540857215
>5k	5.813238918868541E-4	0.9741269855378747
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	8802	0.5344044899205679	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7261	0.4408442400946652	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	4646	0.28207717111690056	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4477	0.2718165077680507	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	3973	0.24121665967443948	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	3912	0.23751310663136352	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	3814	0.23156313616871688	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	3367	0.2044239851809307	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	3247	0.1971383070634042	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3238	0.19659188120458973	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	3137	0.19045976878900495	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	3109	0.18875977722824874	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	3064	0.1860276479341763	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	3030	0.18396337246754382	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	3008	0.182627664812664	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	2981	0.18098838723622052	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	2973	0.18050267536171874	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	2775	0.16848130646780005	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	2625	0.15937420882089193	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	2585	0.1569456494483831	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	2560	0.1554277998405651	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	2475	0.1502671111739838	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	2473	0.15014568320535837	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2393	0.14528856446034072	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	2320	0.1408564436055121	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	2303	0.13982430587219585	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2294	0.13927788001338137	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	2270	0.13782074438987607	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	2245	0.13630289478205806	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	2174	0.13199220189585487	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	2159	0.13108149213116407	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	2157	0.13096006416253864	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	2149	0.13047435228803686	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	2105	0.12780293697827713	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	2091	0.12695294119789904	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	2067	0.12549580557439374	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	1999	0.12136725464112874	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	1981	0.12027440292349978	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	1947	0.11821012745686728	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	1871	0.11359586464910049	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	1839	0.11165301715109342	No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC	1837	0.11153158918246799	No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT	1830	0.11110659129227896	No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT	1719	0.10436733903356693	No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA	1697	0.1030316313786871	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	1660	0.10078521395911642	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	3.0356992156360367E-5	0.0
12-13	0.0	0.0	0.0	6.0713984312720734E-5	6.0713984312720734E-5
14-15	0.0	0.0	0.0	6.0713984312720734E-5	6.0713984312720734E-5
16-17	0.0	0.0	0.0	6.0713984312720734E-5	6.0713984312720734E-5
18-19	0.0	0.0	0.0	6.0713984312720734E-5	6.0713984312720734E-5
20-21	0.0	0.0	0.0	9.10709764690811E-5	6.0713984312720734E-5
22-23	0.0	0.0	0.0	2.428559372508829E-4	6.0713984312720734E-5
24-25	0.0	0.0	0.0	3.0356992156360365E-4	6.0713984312720734E-5
26-27	0.0	0.0	0.0	3.9464089803268476E-4	6.0713984312720734E-5
28-29	0.0	0.0	0.0	0.0011535657019416938	6.0713984312720734E-5
30-31	0.0	0.0	0.0	0.0037946240195450456	6.0713984312720734E-5
32-33	0.0	0.0	0.0	0.010807089207664291	6.0713984312720734E-5
34-35	0.0	0.0	0.0	0.02039989872907417	6.0713984312720734E-5
36-37	0.0	0.0	0.0	0.03903909191307943	6.0713984312720734E-5
38-39	0.0	0.0	0.0	0.07343356402623573	6.0713984312720734E-5
40-41	0.0	0.0	0.0	0.11292801082166057	6.0713984312720734E-5
42-43	0.0	0.0	0.0	0.1551242299190015	6.0713984312720734E-5
44-45	0.0	0.0	0.0	0.20600254877306146	6.0713984312720734E-5
46-47	0.0	0.0	0.0	0.27281828850921064	6.0713984312720734E-5
48-49	0.0	0.0	0.0	0.3548428813156963	6.0713984312720734E-5
50-51	0.0	0.0	0.0	0.44548885989458836	6.0713984312720734E-5
52-53	0.0	0.0	0.0	0.536772335308764	6.0713984312720734E-5
54-55	0.0	0.0	0.0	0.6390146848913857	6.0713984312720734E-5
56-57	0.0	0.0	0.0	0.7680015445637609	6.0713984312720734E-5
58-59	0.0	0.0	0.0	0.9311400204120415	6.0713984312720734E-5
60-61	0.0	0.0	0.0	1.0979213353190853	6.0713984312720734E-5
62-63	0.0	0.0	0.0	1.287166824421836	6.0713984312720734E-5
64-65	0.0	0.0	0.0	1.4802980085206006	6.0713984312720734E-5
66-67	0.0	0.0	0.0	1.6991719219679589	6.0713984312720734E-5
68-69	0.0	0.0	0.0	1.9515903117480953	6.0713984312720734E-5
70-71	0.0	0.0	0.0	2.2414995868413365	6.0713984312720734E-5
72-73	0.0	0.0	0.0	2.5458891471931624	6.0713984312720734E-5
74-75	0.0	0.0	0.0	2.8450573048940937	6.0713984312720734E-5
76-77	0.0	0.0	0.0	3.2050001608920584	6.0713984312720734E-5
78-79	0.0	0.0	0.0	3.585555414564192	6.0713984312720734E-5
80-81	0.0	0.0	0.0	4.002447987847489	6.0713984312720734E-5
82-83	0.0	0.0	0.0	4.443170799973529	6.0713984312720734E-5
84-85	0.0	0.0	0.0	4.877731142691827	1.2142796862544147E-4
86-87	0.0	0.0	0.0	5.343043118464519	1.5178496078180182E-4
88	0.0	0.0	0.0	5.725571576626816	1.821419529381622E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	1985	0.0	42.185265	94
GGTATCA	3755	0.0	36.817474	1
GTATCAA	6235	0.0	36.20107	1
TATTCTC	775	0.0	32.74897	5
CAACGCA	6665	0.0	32.65024	5
ATCAACG	6820	0.0	31.92079	3
CTAACTG	655	0.0	31.584587	4
TACCCCG	120	1.6527702E-7	31.333889	5
CTGTGCG	2940	0.0	30.381144	9
TAATAAT	980	0.0	29.267174	3
TAAGACG	65	0.006143451	28.934132	4
TATCAAC	7885	0.0	28.921247	2
TAGTACT	3075	0.0	28.593039	4
ACTGTGC	3125	0.0	28.573906	8
AACGCAG	8165	0.0	28.262104	6
GTACTAG	2080	0.0	28.25942	1
GTGGTAT	985	0.0	27.68907	1
ACGCAGA	7855	0.0	27.642351	7
GTACTGT	3210	0.0	27.525297	6
TACTGTG	3430	0.0	27.26705	7
>>END_MODULE