##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146450_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4638887 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.146562957881923 31.0 30.0 34.0 26.0 34.0 2 30.201797758815854 31.0 30.0 34.0 26.0 34.0 3 30.47499777425059 31.0 30.0 34.0 26.0 34.0 4 33.95869979156638 35.0 35.0 37.0 28.0 37.0 5 34.1578598487094 37.0 35.0 37.0 30.0 37.0 6 34.27756399325959 37.0 35.0 37.0 30.0 37.0 7 34.220649263497904 37.0 35.0 37.0 30.0 37.0 8 34.16303048554535 37.0 35.0 37.0 30.0 37.0 9 35.52476639331805 39.0 35.0 39.0 29.0 39.0 10-11 35.44472413318108 39.0 35.0 39.0 28.5 39.0 12-13 35.423182435786856 39.0 35.0 39.0 29.0 39.0 14-15 36.39394600472053 39.5 36.0 41.0 27.5 41.0 16-17 36.21699730991507 39.0 35.5 41.0 27.0 41.0 18-19 36.15546918905332 39.0 35.0 41.0 27.0 41.0 20-21 36.48207942982875 39.0 36.0 41.0 28.0 41.0 22-23 36.19367027047652 39.0 35.0 41.0 27.0 41.0 24-25 36.279020161517195 39.0 35.0 41.0 27.5 41.0 26-27 36.337348053530945 39.0 35.0 41.0 28.0 41.0 28-29 36.17859618050623 39.0 35.0 41.0 27.5 41.0 30-31 36.11655543668125 39.0 35.0 41.0 27.5 41.0 32-33 36.21211531990325 39.0 35.0 41.0 28.0 41.0 34-35 36.25746736232204 39.0 35.0 41.0 28.0 41.0 36-37 36.179239438253184 39.0 35.0 41.0 28.0 41.0 38-39 36.01652702469364 39.0 35.0 41.0 27.0 41.0 40-41 35.96797346001314 39.0 35.0 41.0 27.0 41.0 42-43 35.80542326208851 39.0 34.0 41.0 27.0 41.0 44-45 35.613334728783 38.0 34.0 40.0 27.0 41.0 46-47 35.488384390479865 38.0 34.0 40.0 26.5 41.0 48-49 35.355754839469036 38.0 33.5 40.0 26.0 41.0 50-51 35.14019041636496 38.0 33.0 40.0 26.0 41.0 52-53 34.94900209899487 38.0 33.0 40.0 26.0 41.0 54-55 34.75214216254891 37.0 33.0 40.0 26.0 41.0 56-57 34.593920373572374 37.0 33.0 40.0 26.0 41.0 58-59 34.32863130746664 36.0 33.0 40.0 25.5 41.0 60-61 34.08332785428919 36.0 32.0 39.5 25.0 41.0 62-63 33.811629061022614 35.0 32.0 39.0 25.0 41.0 64-65 33.525463112164616 35.0 32.0 39.0 24.5 41.0 66-67 33.24507829140913 35.0 31.5 38.5 24.0 40.0 68-69 32.95997164837169 35.0 31.0 38.0 23.0 40.0 70-71 32.913459952786084 35.0 31.0 37.0 24.0 40.0 72-73 32.71152918792805 35.0 31.0 37.0 24.0 40.0 74-75 32.45374375362021 35.0 31.0 36.0 24.0 39.0 76-77 32.229206165185744 35.0 31.0 36.0 23.0 39.0 78-79 32.01443999390371 35.0 31.0 36.0 23.0 39.0 80-81 31.80361830326973 35.0 31.0 35.0 23.0 37.5 82-83 31.574397910533282 34.0 30.0 35.0 23.0 37.0 84-85 31.394687669693184 34.0 30.0 35.0 23.0 37.0 86-87 31.242888110876596 34.0 30.5 35.0 22.0 36.0 88-89 31.26411626323297 34.0 31.0 35.0 23.0 36.0 90-91 31.189652173032023 34.0 31.0 35.0 22.5 36.0 92-93 31.049344271589284 34.0 30.5 35.0 21.0 35.5 94-95 31.034163906126622 34.0 31.0 35.0 21.0 35.0 96-97 30.948910374406623 34.0 30.0 35.0 20.5 35.0 98-99 30.878009638950033 34.0 30.0 35.0 20.0 35.0 100 30.366834544579337 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.004310840821617 1101 2 1.1319695371837213 1101 3 1.097560313442056 1101 4 1.220090973088034 1101 5 1.1316605112282971 1101 6 0.8232303209153642 1101 7 0.8400143475876973 1101 8 0.8972055734881934 1101 9 1.124525060740531 1101 10-11 1.1556597809879605 1101 12-13 1.0702881934661193 1101 14-15 1.3550329000611043 1101 16-17 1.4439505804486785 1101 18-19 1.5647010242376211 1101 20-21 1.2542015707861154 1101 22-23 1.5946602790414275 1101 24-25 1.4266502494995095 1101 26-27 1.3648273178319457 1101 28-29 1.5266797906801202 1101 30-31 1.543832757114302 1101 32-33 1.3210268547339012 1101 34-35 1.2039004776392446 1101 36-37 1.2072424820478886 1101 38-39 1.3214457574580862 1101 40-41 1.2159069416486687 1101 42-43 1.1913592993360211 1101 44-45 1.2294056696090578 1101 46-47 1.2441199542049475 1101 48-49 1.2445764374838433 1101 50-51 1.272525463829993 1101 52-53 1.2729820398058251 1101 54-55 1.30907409963649 1101 56-57 1.1747253874667791 1101 58-59 1.1062811367688425 1101 60-61 1.1228224986700255 1101 62-63 1.1330490683014531 1101 64-65 1.0922024434420337 1101 66-67 1.089420433088499 1101 68-69 1.0870544017431385 1101 70-71 1.0627296730424618 1101 72-73 1.005865179033492 1101 74-75 1.0443687340082874 1101 76-77 1.09135759629185 1101 78-79 0.9775316710783954 1101 80-81 0.992836066255645 1101 82-83 0.9993191354198423 1101 84-85 1.0547687704060635 1101 86-87 1.0130403191886401 1101 88-89 1.047427816159182 1101 90-91 1.0884308413049872 1101 92-93 1.1397241035538563 1101 94-95 1.075173641341685 1101 96-97 1.0345285336155996 1101 98-99 1.0824496690505576 1101 100 1.1613847241526152 1102 1 0.8292647217450551 1102 2 0.936329016824633 1102 3 0.9129732710779415 1102 4 1.0611343086612592 1102 5 0.958119396669268 1102 6 0.7148171443239733 1102 7 0.7608204058600663 1102 8 0.7316399761800909 1102 9 0.9472365352674501 1102 10-11 0.9545063636461748 1102 12-13 0.9188414512472178 1102 14-15 1.1097511395581634 1102 16-17 1.333257246511323 1102 18-19 1.3488788463098444 1102 20-21 1.1072522250100647 1102 22-23 1.3883085559622543 1102 24-25 1.2939141361862667 1102 26-27 1.1230211614071166 1102 28-29 1.294316405074305 1102 30-31 1.3683273348195968 1102 32-33 1.2423324839038088 1102 34-35 1.14565076265891 1102 36-37 1.0800771505064688 1102 38-39 1.024552157250703 1102 40-41 0.9948738743336918 1102 42-43 1.0876700248840976 1102 44-45 1.1125224383291297 1102 46-47 1.0678746133012922 1102 48-49 1.0075533263525998 1102 50-51 1.1714863060285055 1102 52-53 1.1580807333712997 1102 54-55 1.1323077956306093 1102 56-57 1.0150300627960291 1102 58-59 1.0206229741327206 1102 60-61 1.0530738444913723 1102 62-63 0.9946666713174963 1102 64-65 0.9597633042643494 1102 66-67 0.9279722059989268 1102 68-69 0.9830404691260668 1102 70-71 0.9421858580321825 1102 72-73 0.8507830203838651 1102 74-75 0.833753670372765 1102 76-77 0.9264148896494433 1102 78-79 0.8628694539903066 1102 80-81 0.9446054532557291 1102 82-83 0.8789389078571226 1102 84-85 0.8307961624949698 1102 86-87 0.915427689230988 1102 88-89 0.885547969881042 1102 90-91 0.87067581476607 1102 92-93 0.971970209543418 1102 94-95 0.9472709079212756 1102 96-97 0.9551131196512728 1102 98-99 0.9445039247150895 1102 100 0.942371332632483 1103 1 0.6931264640692127 1103 2 0.8801640568319264 1103 3 0.8329909387004157 1103 4 0.8664979694951995 1103 5 0.86752261068861 1103 6 0.655039568405158 1103 7 0.7013330000978542 1103 8 0.667808951816852 1103 9 0.8936963811234477 1103 10-11 0.9642845023406466 1103 12-13 0.8265357704888032 1103 14-15 0.955980186827432 1103 16-17 1.1957313556765854 1103 18-19 1.3062889688924813 1103 20-21 1.0211169881524285 1103 22-23 1.1917693284548747 1103 24-25 1.0957278687501244 1103 26-27 0.931487406956073 1103 28-29 1.1413802782942213 1103 30-31 1.24167348732432 1103 32-33 1.022646419849238 1103 34-35 0.9746487297517277 1103 36-37 0.9611035755742989 1103 38-39 1.0247659110536702 1103 40-41 0.9513337641013351 1103 42-43 0.9564325589452736 1103 44-45 0.9774414417892103 1103 46-47 0.9897300084245657 1103 48-49 0.9782063424540937 1103 50-51 1.0233452044933244 1103 52-53 1.0223853271398937 1103 54-55 0.9979623695859701 1103 56-57 0.8871837618245237 1103 58-59 0.9020885408682489 1103 60-61 0.8541830727856379 1103 62-63 0.818236578880466 1103 64-65 0.8021135149620093 1103 66-67 0.8712062369368496 1103 68-69 0.8807131419465222 1103 70-71 0.7812769162941962 1103 72-73 0.7177743757964805 1103 74-75 0.7388004456102166 1103 76-77 0.7143442401563718 1103 78-79 0.7460429817502572 1103 80-81 0.7402691330726263 1103 82-83 0.7187907227177597 1103 84-85 0.6954621692756362 1103 86-87 0.7562703677947908 1103 88-89 0.739602601920911 1103 90-91 0.7678411819257036 1103 92-93 0.8349422615996112 1103 94-95 0.8209054640222604 1103 96-97 0.762172350350582 1103 98-99 0.8522173480450839 1103 100 0.911290873530703 1104 1 0.6705493094187425 1104 2 0.7716054533739971 1104 3 0.7466675606686124 1104 4 0.8715009445985373 1104 5 0.761889728108244 1104 6 0.47232952783151916 1104 7 0.5409417046201526 1104 8 0.5049963456445568 1104 9 0.6185086923178389 1104 10-11 0.66853043672063 1104 12-13 0.5932210657391863 1104 14-15 0.7918282776660419 1104 16-17 0.8669236336575921 1104 18-19 0.816347973589231 1104 20-21 0.7739913259462909 1104 22-23 0.9752826722542522 1104 24-25 0.8418927761856096 1104 26-27 0.6794523177546381 1104 28-29 0.8860056178682783 1104 30-31 0.9574729169391745 1104 32-33 0.7201792771660394 1104 34-35 0.6257818267785922 1104 36-37 0.6531462428309283 1104 38-39 0.8138495711216223 1104 40-41 0.7254859406877472 1104 42-43 0.6448310538286464 1104 44-45 0.6751505311806838 1104 46-47 0.6467608147737991 1104 48-49 0.6494821401049222 1104 50-51 0.7631987295298757 1104 52-53 0.7866394605278515 1104 54-55 0.7483686479555587 1104 56-57 0.617171582843433 1104 58-59 0.5658252068440675 1104 60-61 0.6038565660836994 1104 62-63 0.5933167405429387 1104 64-65 0.6629401408909317 1104 66-67 0.6962924967001385 1104 68-69 0.6393783710011149 1104 70-71 0.5468124612324416 1104 72-73 0.5057241385884623 1104 74-75 0.5234883828982362 1104 76-77 0.6184467678698056 1104 78-79 0.5563206570488788 1104 80-81 0.6250776800572702 1104 82-83 0.6087652322511623 1104 84-85 0.5585146273268862 1104 86-87 0.5538934456014886 1104 88-89 0.5524525462985288 1104 90-91 0.5856417813224155 1104 92-93 0.5786509107527742 1104 94-95 0.5292294779988644 1104 96-97 0.4691212527228217 1104 98-99 0.5127665407760666 1104 100 0.722529480870346 1105 1 0.42648206919497156 1105 2 0.6646342739277102 1105 3 0.6337134353697316 1105 4 0.7587037060780304 1105 5 0.5933064296669954 1105 6 0.3608825499485704 1105 7 0.4129901764081936 1105 8 0.4606084453136319 1105 9 0.47631457679350575 1105 10-11 0.5666117107406166 1105 12-13 0.5222175497196986 1105 14-15 0.6840909354621232 1105 16-17 0.7939522110044805 1105 18-19 0.8188381301432486 1105 20-21 0.6933714006517491 1105 22-23 0.8948503237997016 1105 24-25 0.7339453244440293 1105 26-27 0.5634587450429009 1105 28-29 0.7563377173386527 1105 30-31 0.7851825176410614 1105 32-33 0.6718566948811144 1105 34-35 0.6282978419211105 1105 36-37 0.5197389947638413 1105 38-39 0.7036743764625939 1105 40-41 0.5505803874810127 1105 42-43 0.5319462363518355 1105 44-45 0.5930416360371922 1105 46-47 0.49901019894811327 1105 48-49 0.5835121174221811 1105 50-51 0.6316322642131524 1105 52-53 0.5567148522633403 1105 54-55 0.5130893384533692 1105 56-57 0.516389477925749 1105 58-59 0.567265521717303 1105 60-61 0.5631657208882501 1105 62-63 0.4818850056174213 1105 64-65 0.40915494539557784 1105 66-67 0.49688536948792716 1105 68-69 0.4897651712532536 1105 70-71 0.4196858134874546 1105 72-73 0.35377115338113896 1105 74-75 0.38548159794486025 1105 76-77 0.4199125466478151 1105 78-79 0.417395892203821 1105 80-81 0.5206999128905885 1105 82-83 0.5717054007171392 1105 84-85 0.44995384218936607 1105 86-87 0.4797769808325576 1105 88-89 0.45265280405422104 1105 90-91 0.48359239291546174 1105 92-93 0.5273651723971859 1105 94-95 0.45846026290705666 1105 96-97 0.5132785948165015 1105 98-99 0.5483725327145841 1105 100 0.5952697209373987 1106 1 0.640396307362991 1106 2 0.6880738620133613 1106 3 0.6615395152788324 1106 4 0.7750803672411877 1106 5 0.6326092112584263 1106 6 0.4796681406875507 1106 7 0.5714346067783893 1106 8 0.5190595165112271 1106 9 0.6517109505244605 1106 10-11 0.6380085963846938 1106 12-13 0.5639249675798013 1106 14-15 0.7280455363066523 1106 16-17 0.8644725580839534 1106 18-19 0.8677501994750259 1106 20-21 0.7034607772457449 1106 22-23 1.010469810118252 1106 24-25 0.8563726806565839 1106 26-27 0.7142576215588363 1106 28-29 0.8692641752229662 1106 30-31 0.8383598688494089 1106 32-33 0.6903085884094793 1106 34-35 0.7006450628047105 1106 36-37 0.645573399357076 1106 38-39 0.7769548763838046 1106 40-41 0.6683832685101905 1106 42-43 0.6966863265587904 1106 44-45 0.7184810493862912 1106 46-47 0.6617366879300874 1106 48-49 0.6723989285537968 1106 50-51 0.7113721565227564 1106 52-53 0.6623259374636206 1106 54-55 0.7203042533395063 1106 56-57 0.6590353152640418 1106 58-59 0.6132502472553227 1106 60-61 0.5586056191120647 1106 62-63 0.5781370567719861 1106 64-65 0.5324952669101322 1106 66-67 0.6502001624183009 1106 68-69 0.6214538355006027 1106 70-71 0.639830988751676 1106 72-73 0.5652030286051328 1106 74-75 0.5424284798143901 1106 76-77 0.6050602121349726 1106 78-79 0.62119679824184 1106 80-81 0.6483222755502673 1106 82-83 0.6456971259178168 1106 84-85 0.6447975086932338 1106 86-87 0.6333424204576019 1106 88-89 0.6548770375827395 1106 90-91 0.6361113258235775 1106 92-93 0.685447894260296 1106 94-95 0.7046530007483263 1106 96-97 0.5858709254608172 1106 98-99 0.6097449828385955 1106 100 0.6267330075195616 1107 1 0.5279023642269145 1107 2 0.6792687892945359 1107 3 0.5679612161854237 1107 4 0.6528483789127861 1107 5 0.5863288051386562 1107 6 0.35984829220360126 1107 7 0.4400671110753649 1107 8 0.465546668005409 1107 9 0.5588531779523294 1107 10-11 0.5636652731201792 1107 12-13 0.5006150795298652 1107 14-15 0.6497456143250204 1107 16-17 0.8011815614837516 1107 18-19 0.9338298391985091 1107 20-21 0.6478781549932009 1107 22-23 0.8349249906509115 1107 24-25 0.5748347310483624 1107 26-27 0.5259100334666797 1107 28-29 0.6650202607554974 1107 30-31 0.768968425416702 1107 32-33 0.6609986698680785 1107 34-35 0.5433647952222316 1107 36-37 0.4879255770772204 1107 38-39 0.5293867362503377 1107 40-41 0.3379764527402713 1107 42-43 0.5680870910990805 1107 44-45 0.4843894729180107 1107 46-47 0.4951939756879682 1107 48-49 0.4895253865925753 1107 50-51 0.48473093697030833 1107 52-53 0.512586838327266 1107 54-55 0.4692136864037906 1107 56-57 0.45594775637740526 1107 58-59 0.4938529706732311 1107 60-61 0.5550435865511645 1107 62-63 0.4481078711392996 1107 64-65 0.5573471347442265 1107 66-67 0.5121184134810264 1107 68-69 0.485056077020829 1107 70-71 0.49091830768376354 1107 72-73 0.459188521297456 1107 74-75 0.48941360329487793 1107 76-77 0.41237372204039247 1107 78-79 0.41390141749958786 1107 80-81 0.4707313795056045 1107 82-83 0.48601963279331173 1107 84-85 0.43199112749294244 1107 86-87 0.5304428480672243 1107 88-89 0.5390721193098038 1107 90-91 0.5607665900088996 1107 92-93 0.6390374022805467 1107 94-95 0.6119200111730905 1107 96-97 0.610782119075644 1107 98-99 0.6533794259948884 1107 100 0.5471486326707762 1108 1 0.43218526970496995 1108 2 0.5691042671407693 1108 3 0.582437610604142 1108 4 0.6553520901865681 1108 5 0.5881806870755497 1108 6 0.27823184127564105 1108 7 0.3287827441794846 1108 8 0.3965291345676718 1108 9 0.4492928712987805 1108 10-11 0.5153728115655412 1108 12-13 0.37847294425773725 1108 14-15 0.6085993408382961 1108 16-17 0.7831853448424297 1108 18-19 0.8265082258169372 1108 20-21 0.46609601097892295 1108 22-23 0.6918559866557388 1108 24-25 0.5528448016813456 1108 26-27 0.5063010258279306 1108 28-29 0.6676650254660075 1108 30-31 0.7529616190902786 1108 32-33 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2216 2 -0.7255407396542886 2216 3 -0.9703723141544387 2216 4 -1.0213886490638515 2216 5 -1.0639631927987665 2216 6 -0.9599229403891556 2216 7 -1.1926387136367893 2216 8 -0.9226266018061651 2216 9 -1.0849381184705464 2216 10-11 -1.1755242077590538 2216 12-13 -1.4037802217787672 2216 14-15 -1.805230599278019 2216 16-17 -1.5665718103258115 2216 18-19 -3.3800659734705505 2216 20-21 -1.750160173911766 2216 22-23 -1.26803267692884 2216 24-25 -1.3195209462515152 2216 26-27 -1.351941207760106 2216 28-29 -1.2819084931182516 2216 30-31 -1.508668849588986 2216 32-33 -1.4121167057205781 2216 34-35 -1.6883873465813508 2216 36-37 -1.74263261950788 2216 38-39 -1.713025000542082 2216 40-41 -1.6986740422600448 2216 42-43 -1.7064064736398379 2216 44-45 -1.668742074336997 2216 46-47 -1.7861836824012087 2216 48-49 -1.7932459642041039 2216 50-51 -1.7307388722074535 2216 52-53 -1.6827250915315588 2216 54-55 -1.6248309775458978 2216 56-57 -1.5543868634942086 2216 58-59 -1.6661029683883726 2216 60-61 -1.7049849879609908 2216 62-63 -1.648957673789326 2216 64-65 -1.647830539448087 2216 66-67 -1.714777003461876 2216 68-69 -1.7038540828628221 2216 70-71 -1.7380512453855133 2216 72-73 -1.6536264964245717 2216 74-75 -1.8812718252457188 2216 76-77 -1.6474967333187855 2216 78-79 -1.7419694627084539 2216 80-81 -1.6589610162137056 2216 82-83 -1.8144118115043817 2216 84-85 -1.7132202433812687 2216 86-87 -1.7734160815534779 2216 88-89 -1.6277622598712327 2216 90-91 -1.6968268981544306 2216 92-93 -1.573472084012085 2216 94-95 -1.4911813855175424 2216 96-97 -1.5144759166493529 2216 98-99 -1.4845844173112575 2216 100 -1.5497057726521675 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19350.0 1 2888.0 2 498.0 3 503.0 4 454.0 5 256.0 6 259.0 7 280.0 8 519.0 9 935.0 10 1861.0 11 4240.0 12 7695.0 13 11199.0 14 14148.0 15 17144.0 16 20443.0 17 23229.0 18 26619.0 19 29725.0 20 33013.0 21 36967.0 22 42393.0 23 47775.0 24 53766.0 25 60866.0 26 69042.0 27 78573.0 28 91973.0 29 108066.0 30 127956.0 31 152477.0 32 185104.0 33 231633.0 34 313965.0 35 414573.0 36 604763.0 37 877524.0 38 701216.0 39 224821.0 40 176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.384091830420964 21.19348283382994 12.339603959102684 24.082821376646415 2 15.351386629664468 19.873393929629994 40.93911700559364 23.8361024351119 3 18.549406570098203 26.839511392918354 28.79915573354313 25.81192630344032 4 12.091252430578267 16.669316172046607 40.583867241154515 30.65556415622061 5 13.026276076996124 38.749711500530665 35.16792292939655 13.056089493076662 6 29.578617463923706 38.8136414916791 18.287435381541346 13.320305662855848 7 26.762583786833382 31.67678528948245 24.070723908707503 17.489907014976662 8 25.868448973781703 35.430373825599766 20.00931221956905 18.69186498104948 9 26.040830584193035 14.908242566919824 21.735767889925754 37.31515895896138 10-11 25.664102314591968 25.78967227194717 27.674023511533296 20.872201901927568 12-13 25.83885970387323 23.573156142723395 27.623909896320953 22.964074257082423 14-15 23.20741431979417 24.80109952218809 25.04293112296202 26.948555035055723 16-17 22.203215495965026 27.361331086335184 26.44167210644807 23.993781311251723 18-19 21.56315100815682 28.217002267438552 27.941572144910182 22.278274579494443 20-21 23.340646856727254 27.360426311086684 26.803568398383888 22.495358433802174 22-23 23.88084518045105 26.940641396233634 26.467749619201726 22.710763804113597 24-25 23.314839313540762 26.92585961542567 27.297686826242646 22.461614244790926 26-27 23.178367502676426 27.23447700115507 26.195269773537866 23.391885722630633 28-29 22.228116044153392 27.828536152313692 26.89300026305514 23.050347540477773 30-31 23.138291619423526 27.401057560157472 26.827964183153686 22.632686637265316 32-33 23.504501312634556 27.54444700867758 25.840860802229365 23.110190876458496 34-35 23.47622698347768 27.346605731747292 26.823515388595563 22.353651896179468 36-37 23.096318129687308 26.93933895450828 26.448886057516503 23.51545685828791 38-39 23.329432772986305 27.022842227571797 27.088687211833374 22.55903778760853 40-41 22.943314123610968 26.11747003881537 27.763926326220794 23.17528951135287 42-43 22.915033635962168 26.365912668416875 27.037168889647496 23.681884805973468 44-45 23.07135221595085 27.322060035022723 26.891532193777955 22.715055555248473 46-47 22.940685062245972 27.067237526582943 26.407321642583515 23.584755768587577 48-49 23.681911515294274 26.616315806638568 26.74840501013666 22.953367667930493 50-51 23.273264812190327 26.463576893826186 26.73497107577819 23.5281872182053 52-53 22.703653618185086 27.07652031822605 26.545587333372346 23.67423873021652 54-55 22.669071000825767 26.996558218769845 26.529002642022242 23.805368138382146 56-57 23.8497732551613 26.348333209808 26.232273572643084 23.56961996238762 58-59 22.66284927388482 26.838130756213936 27.20873037339103 23.29028959651021 60-61 22.723665487907788 27.466183460766143 26.487715350318496 23.32243570100757 62-63 23.238454996706746 26.685533046375692 26.005797299931338 24.070214656986224 64-65 22.27378645321553 26.896405922410853 26.608382121112168 24.22142550326145 66-67 23.1027676293172 26.42734263288223 26.50296666387822 23.966923073922356 68-69 22.41064386661807 26.56647642420914 26.823221913659427 24.199657795513367 70-71 22.336132457691292 26.206539920789968 26.978679523595478 24.478648097923262 72-73 23.021793545531764 26.30837022737218 27.39939127274193 23.270444954354126 74-75 23.17442734818966 26.605033746467438 26.86996434901876 23.35057455632414 76-77 22.782442174078678 26.52648340298867 26.525172354162613 24.165902068770038 78-79 22.72628002136047 26.551427649538727 26.559209675638 24.1630826534628 80-81 22.2714594515055 26.909879508568242 27.330898994440116 23.48776204548614 82-83 23.198219405733926 25.801594113402686 28.320483562974243 22.67970291788915 84-85 23.359607556581764 26.317378471377488 26.953360824514363 23.369653147526385 86-87 21.942548560616206 26.953978935762073 27.531552009293865 23.571920494327856 88-89 23.081698969244737 25.642158207381016 27.29482569162478 23.981317131749467 90-91 22.657300120641537 25.789403312035724 27.422951401325935 24.130345165996804 92-93 22.89513524375215 26.026760305688224 27.418192897503168 23.65991155305645 94-95 21.75106619828591 26.07298479397186 28.069803771565756 24.106145236176474 96-97 22.14213141106669 25.89363082089201 27.607109730646716 24.357128037394585 98-99 21.493764639324528 26.555396903850966 27.521036605829842 24.429801850994668 100 22.316745098765605 25.78446445168172 26.6887562256273 25.210034223925366 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15956.0 1 9290.0 2 2418.0 3 1695.0 4 833.5 5 404.0 6 309.5 7 275.0 8 260.5 9 276.5 10 261.5 11 235.5 12 222.5 13 227.5 14 254.0 15 308.0 16 345.0 17 367.5 18 443.5 19 564.0 20 777.5 21 1041.5 22 1353.5 23 1932.5 24 2770.0 25 3959.5 26 5671.0 27 7867.0 28 11813.0 29 20212.0 30 32272.0 31 44196.5 32 59905.0 33 75825.5 34 89491.5 35 104027.0 36 117140.5 37 139587.0 38 150368.0 39 142231.0 40 133800.0 41 125316.5 42 125149.0 43 134260.0 44 147596.0 45 173793.5 46 200026.0 47 217785.5 48 228556.5 49 224398.0 50 213545.0 51 216481.0 52 217535.5 53 206290.0 54 192592.0 55 165103.0 56 143067.0 57 117574.0 58 92152.5 59 86726.0 60 71447.0 61 49187.0 62 36361.5 63 29705.0 64 21436.5 65 10296.0 66 5325.0 67 3489.0 68 2536.0 69 1922.0 70 1506.5 71 1133.0 72 816.0 73 656.5 74 540.5 75 406.0 76 276.0 77 172.0 78 115.0 79 80.0 80 55.5 81 48.5 82 34.0 83 25.0 84 20.0 85 16.0 86 20.0 87 19.5 88 17.0 89 13.0 90 9.5 91 5.5 92 2.0 93 3.0 94 2.5 95 2.5 96 4.0 97 3.5 98 1.0 99 2.5 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.985689024112896 2 0.4480169488931289 3 0.48147324994120355 4 0.46720258544775933 5 0.43460856019989275 6 0.48630199442236904 7 0.5023834380962502 8 0.5054229602919839 9 0.5197582954704436 10-11 0.5330157858986434 12-13 0.5313127911932323 14-15 0.5285535086325663 16-17 0.5331235703736694 18-19 0.5313666834307453 20-21 0.5303858447080086 22-23 0.4942133748892784 24-25 0.5284133888150325 26-27 0.508527153172733 28-29 0.5148864371992679 30-31 0.5177858395774676 32-33 0.5199954213155009 34-35 0.5319056058058754 36-37 0.54965770884266 38-39 0.539353513030173 40-41 0.5443331557763749 42-43 0.5421451309333467 44-45 0.5517810630006723 46-47 0.5496145950526495 48-49 0.5217307513634197 50-51 0.5374996200597256 52-53 0.529372670642764 54-55 0.5388577044450533 56-57 0.5270445259822022 58-59 0.5333822531137319 60-61 0.5266133880820981 62-63 0.5206529066131595 64-65 0.5321319532034301 66-67 0.5305798567630554 68-69 0.5510696854655006 70-71 0.5351499185041585 72-73 0.5388792613400586 74-75 0.5356565055367807 76-77 0.5229918297212242 78-79 0.5538505249211718 80-81 0.5279822509149286 82-83 0.5275511130148245 84-85 0.5372517157671657 86-87 0.8377656105871947 88-89 1.098119010012531 90-91 1.0886339762102417 92-93 1.088332179680169 94-95 1.0937860741164853 96-97 1.086790861687297 98-99 1.0825657102662773 100 1.0776722951000963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4638887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.372696296351958 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4624170630635 21.8716196188566 2 12.382011939375944 7.2738615246618705 3 5.077853054715242 4.474507068409616 4 2.5252782488748666 2.966969242719398 5 1.4255648346880492 2.093634147002563 6 0.8966341540786203 1.5801937618012656 7 0.5633131359357506 1.1582217963110502 8 0.41673149812901195 0.9792422185333787 9 0.29491703368701166 0.7796257616798633 >10 1.486703319854236 7.984074676483592 >50 0.17011474663886209 3.5671129693177095 >100 0.2180350652427009 14.433568963097812 >500 0.04524416248553624 9.319148373828007 >1k 0.03334553533751013 17.17984647794224 >5k 0.001395517998706371 2.60101768079684 >10k+ 4.4068989432832767E-4 1.737355718558196 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 17798 0.38366961730259863 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 14569 0.31406240333079893 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 14373 0.30983725190977923 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11846 0.25536297823163184 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 11374 0.24518812378917615 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10571 0.22787793709999832 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9873 0.2128312243863668 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8670 0.18689827969510792 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8229 0.17739168899781346 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7446 0.16051264020873973 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6963 0.1501006599212268 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6494 0.13999047616378668 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6407 0.13811502629833405 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6241 0.1345365817274704 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5853 0.12617250646545175 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5842 0.1259353806203945 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5725 0.12341322390478579 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5715 0.12319765495473377 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5665 0.12211981020447361 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5406 0.11653657439812609 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5390 0.11619166407804285 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5293 0.11410064526253819 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5232 0.11278567466722082 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5065 0.10918567320135197 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 5055 0.10897010425129994 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 4982 0.10739645091592014 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 4970 0.1071377681758577 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 4957 0.10685752854079006 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4893 0.10547788726045709 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4882 0.10524076141539984 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 4786 0.10317129949490039 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4755 0.1025030357497391 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 4673 0.10073537035931249 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.3113790010405515E-5 2 0.0 0.0 0.0 0.0 4.3113790010405515E-5 3 0.0 0.0 0.0 0.0 4.3113790010405515E-5 4 0.0 0.0 0.0 0.0 4.3113790010405515E-5 5 0.0 0.0 0.0 0.0 4.3113790010405515E-5 6 0.0 0.0 0.0 0.0 4.3113790010405515E-5 7 0.0 0.0 0.0 0.0 4.3113790010405515E-5 8 0.0 0.0 0.0 0.0 4.3113790010405515E-5 9 0.0 0.0 0.0 0.0 4.3113790010405515E-5 10-11 0.0 0.0 0.0 0.0 4.3113790010405515E-5 12-13 0.0 0.0 0.0 0.0 2.1556895005202757E-4 14-15 0.0 0.0 0.0 0.0 2.479042925598317E-4 16-17 0.0 0.0 0.0 0.0 3.017965300728386E-4 18-19 0.0 0.0 0.0 0.0 3.017965300728386E-4 20-21 0.0 0.0 0.0 0.0 3.2335342507804136E-4 22-23 0.0 0.0 0.0 3.2335342507804136E-5 3.2335342507804136E-4 24-25 0.0 0.0 0.0 1.508982650364193E-4 3.341318725806427E-4 26-27 0.0 0.0 0.0 2.910180825702372E-4 3.7724566259104823E-4 28-29 0.0 0.0 0.0 0.0013149705953173683 3.8802411009364963E-4 30-31 0.0 0.0 0.0 0.006607188319094645 3.8802411009364963E-4 32-33 0.0 0.0 0.0 0.018334139201924945 3.8802411009364963E-4 34-35 0.0 0.0 0.0 0.03694851803891752 3.8802411009364963E-4 36-37 0.0 0.0 0.0 0.06970421999932311 3.8802411009364963E-4 38-39 0.0 0.0 0.0 0.12512699705769942 3.8802411009364963E-4 40-41 0.0 0.0 0.0 0.18862283129552412 3.8802411009364963E-4 42-43 0.0 0.0 0.0 0.2540372291888119 4.2035945260145374E-4 44-45 0.0 0.0 0.0 0.3347678009832962 4.6347324261185926E-4 46-47 0.0 0.0 0.0 0.43764808239562636 4.7425169011446066E-4 48-49 0.0 0.0 0.0 0.5560708851067078 4.7425169011446066E-4 50-51 0.0 0.0 0.0 0.6846254284702343 4.7425169011446066E-4 52-53 0.0 0.0 0.0 0.8142578165840211 5.604792701352717E-4 54-55 0.0 0.0 0.0 0.9582255398762678 5.604792701352717E-4 56-57 0.0 0.0 0.0 1.1390232182849034 5.820361651404744E-4 58-59 0.0 0.0 1.0778447502601379E-5 1.356478396649886 6.035930601456772E-4 60-61 0.0 0.0 4.3113790010405515E-5 1.5831599260770957 6.035930601456772E-4 62-63 0.0 0.0 4.3113790010405515E-5 1.8271947559834936 6.682637451612854E-4 64-65 0.0 0.0 4.3113790010405515E-5 2.0879685148614313 6.682637451612854E-4 66-67 0.0 0.0 4.3113790010405515E-5 2.3846776177130415 6.790421926638868E-4 68-69 0.0 0.0 4.3113790010405515E-5 2.718399909288586 6.898206401664882E-4 70-71 0.0 0.0 4.3113790010405515E-5 3.090827605845971 6.898206401664882E-4 72-73 0.0 0.0 4.3113790010405515E-5 3.480166686534938 7.329344301768936E-4 74-75 0.0 0.0 4.3113790010405515E-5 3.862887800457308 7.544913251820965E-4 76-77 0.0 0.0 4.3113790010405515E-5 4.304728698931447 7.544913251820965E-4 78-79 0.0 0.0 4.3113790010405515E-5 4.77986422174112 7.544913251820965E-4 80-81 0.0 1.0778447502601379E-5 4.3113790010405515E-5 5.3006895835143215 7.544913251820965E-4 82-83 0.0 2.1556895005202757E-5 4.3113790010405515E-5 5.841530522299854 7.760482201872993E-4 84-85 0.0 2.1556895005202757E-5 4.3113790010405515E-5 6.370989420522639 7.97605115192502E-4 86-87 0.0 2.1556895005202757E-5 4.3113790010405515E-5 6.940026777974975 8.191620101977047E-4 88 0.0 2.1556895005202757E-5 4.3113790010405515E-5 7.397765886515451 8.407189052029075E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4725 0.0 38.98766 94 TACCCCG 325 0.0 36.14304 5 CAACGCA 7805 0.0 33.531296 5 GTACTAG 5630 0.0 32.350018 1 GTATCAA 9440 0.0 31.389418 1 ATCAACG 8480 0.0 30.978432 3 GCATCGT 5570 0.0 30.086475 8 TATTCTC 2530 0.0 29.90015 5 TAGGCAT 6660 0.0 29.56023 5 GGTATCA 6195 0.0 28.333307 1 CTGTGCG 6960 0.0 28.060112 9 TAGTACT 7180 0.0 27.618996 4 AACGCAG 10515 0.0 27.006107 6 ACGCAGA 9740 0.0 26.74748 7 CGCAGAG 9795 0.0 26.454092 8 TATCAAC 11075 0.0 26.436947 2 CTAGGCA 7860 0.0 26.186136 4 ACTGTGC 7630 0.0 26.024004 8 GTACATG 18220 0.0 25.793282 1 AGGGCTA 5220 0.0 25.743263 5 >>END_MODULE