##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146450_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4638887 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.790834094471368 31.0 31.0 34.0 30.0 34.0 2 31.742051487781445 31.0 31.0 34.0 30.0 34.0 3 31.938212549691336 31.0 31.0 34.0 30.0 34.0 4 35.689222651899044 37.0 35.0 37.0 33.0 37.0 5 35.608057493101256 37.0 35.0 37.0 33.0 37.0 6 35.64421250183503 37.0 35.0 37.0 33.0 37.0 7 35.55789201159675 37.0 35.0 37.0 33.0 37.0 8 35.54061933390488 37.0 35.0 37.0 33.0 37.0 9 37.12061384551941 39.0 37.0 39.0 33.0 39.0 10-11 37.0647139065901 39.0 37.0 39.0 33.0 39.0 12-13 37.00461037313476 39.0 37.0 39.0 33.0 39.0 14-15 38.2019934954225 40.0 37.0 41.0 33.0 41.0 16-17 38.20043924329263 40.0 37.0 41.0 33.0 41.0 18-19 38.23339952018663 40.0 37.0 41.0 33.0 41.0 20-21 38.24276017070474 40.0 37.0 41.0 33.0 41.0 22-23 38.21003206156994 40.0 37.0 41.0 33.0 41.0 24-25 38.143005100145785 40.0 37.0 41.0 33.0 41.0 26-27 38.00559110407302 40.0 37.0 41.0 33.0 41.0 28-29 37.93654824961246 40.0 37.0 41.0 33.0 41.0 30-31 37.824639832787476 40.0 37.0 41.0 32.5 41.0 32-33 37.72480403165673 40.0 36.5 41.0 32.0 41.0 34-35 37.64370225013026 40.0 36.0 41.0 32.0 41.0 36-37 37.57347107183253 40.0 36.0 41.0 32.0 41.0 38-39 37.46007479811429 40.0 36.0 41.0 31.5 41.0 40-41 37.339575204138406 39.5 36.0 41.0 31.0 41.0 42-43 37.22714144578215 39.0 36.0 41.0 31.0 41.0 44-45 37.04602558760323 39.0 35.0 41.0 31.0 41.0 46-47 36.83833816171853 39.0 35.0 41.0 30.5 41.0 48-49 36.61435005853775 39.0 35.0 41.0 30.0 41.0 50-51 36.41592541486783 39.0 35.0 41.0 30.0 41.0 52-53 36.22105356306372 38.0 35.0 40.5 29.5 41.0 54-55 36.01967659483837 38.0 35.0 40.0 29.0 41.0 56-57 35.75897311143815 37.5 34.0 40.0 29.0 41.0 58-59 35.48196776511263 37.0 34.0 40.0 28.0 41.0 60-61 35.22069237728791 36.5 34.0 40.0 28.0 41.0 62-63 34.94534885199833 36.0 34.0 39.5 28.0 41.0 64-65 34.67387015462977 35.5 33.5 39.0 28.0 41.0 66-67 34.45292901077349 35.0 33.0 39.0 28.0 41.0 68-69 34.26360611931267 35.0 33.0 38.5 27.0 41.0 70-71 34.063005846014356 35.0 33.0 38.0 27.0 40.0 72-73 33.86345226775302 35.0 33.0 37.0 27.0 40.0 74-75 33.68633327347702 35.0 33.0 37.0 27.0 39.5 76-77 33.5265442982336 35.0 33.0 36.0 27.0 39.0 78-79 33.40625186601872 35.0 33.0 36.0 27.0 39.0 80-81 33.25558695437074 35.0 33.0 36.0 27.0 38.0 82-83 33.06309789395603 35.0 33.0 35.0 27.0 37.0 84-85 32.9249068796028 35.0 33.0 35.0 27.0 37.0 86-87 32.79136665756248 35.0 33.0 35.0 27.0 36.5 88-89 32.68129434926956 35.0 33.0 35.0 27.0 36.0 90-91 32.589641545482785 35.0 33.0 35.0 27.0 36.0 92-93 32.512302412195 35.0 33.0 35.0 27.0 36.0 94-95 32.47258350979448 35.0 33.0 35.0 27.0 35.0 96-97 32.42266119006564 35.0 33.0 35.0 27.0 35.0 98-99 32.381807748281 35.0 33.0 35.0 27.0 35.0 100 31.871205528395066 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5317204640530377 1101 2 0.6007260135913661 1101 3 0.4726483869161342 1101 4 0.36470226002220585 1101 5 0.3634192984561935 1101 6 0.419624958137625 1101 7 0.40191598631269443 1101 8 0.4214881454936972 1101 9 0.5240576532591135 1101 10-11 0.5443954092821173 1101 12-13 0.5443215977630942 1101 14-15 0.7312434629290934 1101 16-17 0.6554510681661583 1101 18-19 0.7113356412961025 1101 20-21 0.6387513879894371 1101 22-23 0.6835056662410963 1101 24-25 0.7424240394293662 1101 26-27 0.7531293083242403 1101 28-29 0.750666431306783 1101 30-31 0.7643404670411869 1101 32-33 0.7965823090011455 1101 34-35 0.7631552946671079 1101 36-37 0.8139055960110184 1101 38-39 0.8158208869238948 1101 40-41 0.8545203886851596 1101 42-43 0.8164471208383617 1101 44-45 0.7889689859557194 1101 46-47 0.8087531323374648 1101 48-49 0.7982866138562912 1101 50-51 0.830125193089323 1101 52-53 0.8333757860081406 1101 54-55 0.8200339252534903 1101 56-57 0.7946637371426846 1101 58-59 0.786364549102565 1101 60-61 0.8824219203244112 1101 62-63 0.8738338157814951 1101 64-65 0.8168865355878339 1101 66-67 0.7520261068507068 1101 68-69 0.7666509354178217 1101 70-71 0.7747981482644164 1101 72-73 0.6740968301513064 1101 74-75 0.6225029915001841 1101 76-77 0.6560127341468416 1101 78-79 0.6365430540048891 1101 80-81 0.6874894638092286 1101 82-83 0.6661604113706048 1101 84-85 0.6442750577839007 1101 86-87 0.6175159961357437 1101 88-89 0.5857480672714672 1101 90-91 0.6389878323277927 1101 92-93 0.6213328745905855 1101 94-95 0.5494653662985982 1101 96-97 0.5886882027395401 1101 98-99 0.6703659218256277 1101 100 0.6657208511127841 1102 1 0.39640344416331885 1102 2 0.5083720908902087 1102 3 0.44689720594060134 1102 4 0.29182279377030085 1102 5 0.29630314381824263 1102 6 0.3073913854632693 1102 7 0.3568916872849073 1102 8 0.3464015070647406 1102 9 0.42037844572704586 1102 10-11 0.43861221550145757 1102 12-13 0.4610606204161769 1102 14-15 0.5385047322166869 1102 16-17 0.6029420142301163 1102 18-19 0.6011506868111667 1102 20-21 0.5573527908147184 1102 22-23 0.5320123645178896 1102 24-25 0.5356646060962831 1102 26-27 0.5486789747316791 1102 28-29 0.6121292554301405 1102 30-31 0.6478473289054207 1102 32-33 0.6063124903818746 1102 34-35 0.6428405020834091 1102 36-37 0.6352781701979282 1102 38-39 0.608629834402258 1102 40-41 0.5653103650530653 1102 42-43 0.6530811455174117 1102 44-45 0.639971021990263 1102 46-47 0.6456174378080846 1102 48-49 0.6714622992247783 1102 50-51 0.6773760431148688 1102 52-53 0.6109824261484746 1102 54-55 0.6413674901259796 1102 56-57 0.6749554764799299 1102 58-59 0.5997905484331199 1102 60-61 0.5831223262729566 1102 62-63 0.5964410976879719 1102 64-65 0.5471924944493622 1102 66-67 0.5546427509926417 1102 68-69 0.4905644001747902 1102 70-71 0.578519636859852 1102 72-73 0.5711625746881168 1102 74-75 0.5516759185326308 1102 76-77 0.5100046453767391 1102 78-79 0.519248337376105 1102 80-81 0.493053198737158 1102 82-83 0.49240663312974675 1102 84-85 0.4945107495448795 1102 86-87 0.46192042457378335 1102 88-89 0.46410695799757207 1102 90-91 0.5083003372039556 1102 92-93 0.4839949205410363 1102 94-95 0.49800453039190273 1102 96-97 0.5002087858155377 1102 98-99 0.47831300002797406 1102 100 0.46971792163910564 1103 1 0.3117338719591274 1103 2 -0.18586341573128706 1103 3 0.16807800718473942 1103 4 0.22636148656509647 1103 5 0.22457786595974483 1103 6 0.25143467769984795 1103 7 0.2900569551133714 1103 8 0.3247627073829449 1103 9 0.3696847755197368 1103 10-11 0.4226233553301171 1103 12-13 0.40949343658644466 1103 14-15 0.4696281338770376 1103 16-17 0.5142988673461275 1103 18-19 0.5215618683710161 1103 20-21 0.47247425975709945 1103 22-23 0.4877267910522747 1103 24-25 0.45892878262957026 1103 26-27 0.5178490643613927 1103 28-29 0.5193991964012881 1103 30-31 0.5716702937155915 1103 32-33 0.617910892866206 1103 34-35 0.621090787647887 1103 36-37 0.5639768920612269 1103 38-39 0.5815885650791799 1103 40-41 0.5915156980161314 1103 42-43 0.6245537951505042 1103 44-45 0.5386030380298195 1103 46-47 0.511755948354427 1103 48-49 0.5538502628314106 1103 50-51 0.5978147830162683 1103 52-53 0.5383571362622277 1103 54-55 0.496166929248119 1103 56-57 0.5226505669512918 1103 58-59 0.493045937085725 1103 60-61 0.6022917269441379 1103 62-63 0.5592095259030145 1103 64-65 0.48930435577659637 1103 66-67 0.5575977522082809 1103 68-69 0.5159660238860369 1103 70-71 0.44631732612824493 1103 72-73 0.461855845148051 1103 74-75 0.45612950803942454 1103 76-77 0.4222201477716112 1103 78-79 0.4256077426670899 1103 80-81 0.42320986286149775 1103 82-83 0.41825233478304114 1103 84-85 0.3552880774890923 1103 86-87 0.3946462478956363 1103 88-89 0.4021199955158252 1103 90-91 0.3987790210539046 1103 92-93 0.3704320423740981 1103 94-95 0.3672137266205695 1103 96-97 0.3957748858857357 1103 98-99 0.43559111007893137 1103 100 0.4586262948474946 1104 1 0.3053648007160845 1104 2 0.3725245320713988 1104 3 0.3409786144218714 1104 4 0.26012941594382255 1104 5 0.24192412645643202 1104 6 0.2838233422826306 1104 7 0.28978963139154246 1104 8 0.24803978624273526 1104 9 0.3326285313785249 1104 10-11 0.335583729020108 1104 12-13 0.37845916829878234 1104 14-15 0.44628509524405047 1104 16-17 0.4588848579623459 1104 18-19 0.41731754461899584 1104 20-21 0.4633819357263107 1104 22-23 0.38845761095510056 1104 24-25 0.4559608967276674 1104 26-27 0.4788438084468041 1104 28-29 0.4906081833811342 1104 30-31 0.4364946197893502 1104 32-33 0.4832656118946943 1104 34-35 0.46689106512160805 1104 36-37 0.5092379535805307 1104 38-39 0.4672766263551722 1104 40-41 0.491388428018368 1104 42-43 0.4640648490218524 1104 44-45 0.4755403945294532 1104 46-47 0.4592717117424385 1104 48-49 0.5101351863610262 1104 50-51 0.48189105141617716 1104 52-53 0.506041709667663 1104 54-55 0.4462632680983063 1104 56-57 0.49276987295095154 1104 58-59 0.5020240483479057 1104 60-61 0.47034204458226014 1104 62-63 0.4132657657897312 1104 64-65 0.4222970666214749 1104 66-67 0.4130822088381265 1104 68-69 0.43481111551253093 1104 70-71 0.40123620416684247 1104 72-73 0.35907471105122113 1104 74-75 0.38952961152480015 1104 76-77 0.34893629718359165 1104 78-79 0.31126095765269923 1104 80-81 0.3782963120508427 1104 82-83 0.304472511068802 1104 84-85 0.2909831485420966 1104 86-87 0.3096635403672394 1104 88-89 0.3222256567616597 1104 90-91 0.29120781357118375 1104 92-93 0.3039680615865592 1104 94-95 0.281528851146426 1104 96-97 0.2392666979191702 1104 98-99 0.2856089536081541 1104 100 0.45392670532463697 1105 1 0.1303918790814258 1105 2 -0.4136769474345421 1105 3 -0.020199110908340145 1105 4 0.08541713867388268 1105 5 0.14717312050630227 1105 6 0.1980066317686422 1105 7 0.2473573548646968 1105 8 0.18499767029159386 1105 9 0.343203486205951 1105 10-11 0.3311045366437071 1105 12-13 0.3384466091906333 1105 14-15 0.373317798056533 1105 16-17 0.35559885060938257 1105 18-19 0.3468116958485936 1105 20-21 0.390968692821815 1105 22-23 0.3818780632340619 1105 24-25 0.4005422244042265 1105 26-27 0.35781152815004447 1105 28-29 0.36323012891191553 1105 30-31 0.4266312099574634 1105 32-33 0.41927612742581033 1105 34-35 0.37667763751874617 1105 36-37 0.3898080126286132 1105 38-39 0.39991878003262826 1105 40-41 0.4233843891790343 1105 42-43 0.4368151024870528 1105 44-45 0.48106953066002234 1105 46-47 0.4179631439958982 1105 48-49 0.4584266175529237 1105 50-51 0.45418519000228486 1105 52-53 0.4364439141531591 1105 54-55 0.46598276399737415 1105 56-57 0.4585567147537546 1105 58-59 0.41185851017046105 1105 60-61 0.37846105274751807 1105 62-63 0.4017624706229981 1105 64-65 0.4386477923733807 1105 66-67 0.4282700381395941 1105 68-69 0.38045824234094283 1105 70-71 0.3379242572471739 1105 72-73 0.372685744870104 1105 74-75 0.40308571285535777 1105 76-77 0.3551661330135403 1105 78-79 0.296367737631698 1105 80-81 0.2936131178673378 1105 82-83 0.3088234907792753 1105 84-85 0.36104374474179934 1105 86-87 0.30241588837810696 1105 88-89 0.2779232598950756 1105 90-91 0.2963957609375285 1105 92-93 0.24981049731316318 1105 94-95 0.2403117836750326 1105 96-97 0.2624498836986291 1105 98-99 0.28224600164340785 1105 100 0.2830081041463437 1106 1 0.2938404226774054 1106 2 0.3282953804018298 1106 3 0.3064356618543229 1106 4 0.24672189323033678 1106 5 0.24338994139026227 1106 6 0.2311135842677885 1106 7 0.24339289268839792 1106 8 0.2272246691430908 1106 9 0.2928035012412593 1106 10-11 0.3034303818182451 1106 12-13 0.3609658610401212 1106 14-15 0.43545068579884827 1106 16-17 0.44831515023555824 1106 18-19 0.44741789928966114 1106 20-21 0.38955451273047714 1106 22-23 0.36183871152832836 1106 24-25 0.3828895794318399 1106 26-27 0.4210067299443878 1106 28-29 0.4224424237279081 1106 30-31 0.41874461607626756 1106 32-33 0.4254744018516945 1106 34-35 0.4085141082092747 1106 36-37 0.44181348137269794 1106 38-39 0.45292056502135836 1106 40-41 0.44393618674202173 1106 42-43 0.4255807857651419 1106 44-45 0.4403206224403391 1106 46-47 0.430771268693654 1106 48-49 0.40225560161008644 1106 50-51 0.3569105876667962 1106 52-53 0.36252289402705884 1106 54-55 0.37936438405254336 1106 56-57 0.42504125180895613 1106 58-59 0.41848668297771496 1106 60-61 0.41184499167761146 1106 62-63 0.40363897596600395 1106 64-65 0.37069315918058265 1106 66-67 0.365985452380329 1106 68-69 0.34685419718624644 1106 70-71 0.3605626841766352 1106 72-73 0.34301947282206413 1106 74-75 0.32021892879637903 1106 76-77 0.3518065590381667 1106 78-79 0.3706941154697674 1106 80-81 0.35035042940678807 1106 82-83 0.33811127758502835 1106 84-85 0.37734049589602137 1106 86-87 0.4269231143563843 1106 88-89 0.3664318827177695 1106 90-91 0.3335011220834332 1106 92-93 0.36902272016648396 1106 94-95 0.38585639807055117 1106 96-97 0.35202610424061476 1106 98-99 0.29556046759773835 1106 100 0.4048169656231231 1107 1 0.09611057836283976 1107 2 -0.4366429506148144 1107 3 -0.026254036472426634 1107 4 0.033637608861653234 1107 5 0.09225635497779194 1107 6 0.13968029329107878 1107 7 0.15438798157432387 1107 8 0.14159668752547105 1107 9 0.17999204917814637 1107 10-11 0.2718660836090976 1107 12-13 0.15963106411657435 1107 14-15 0.20851267162827725 1107 16-17 0.3535365812264146 1107 18-19 0.3420872956050971 1107 20-21 0.24100226063767138 1107 22-23 0.28954013912535714 1107 24-25 0.24719677031303888 1107 26-27 0.2291078807319593 1107 28-29 0.14287465346914274 1107 30-31 0.22453802713631887 1107 32-33 0.29759453291195115 1107 34-35 0.32757866327586527 1107 36-37 0.322576284969891 1107 38-39 0.3005769315393465 1107 40-41 0.2893486136039414 1107 42-43 0.32269264767328565 1107 44-45 0.25743929189896164 1107 46-47 0.3011499063748815 1107 48-49 0.31612865371932486 1107 50-51 0.31215475468312803 1107 52-53 0.3010895566502896 1107 54-55 0.26008178188460107 1107 56-57 0.2896833516366826 1107 58-59 0.2871428844440942 1107 60-61 0.24173776115751622 1107 62-63 0.24545208451097267 1107 64-65 0.30981297670906116 1107 66-67 0.21255473196649177 1107 68-69 0.20159204020691845 1107 70-71 0.2844951018380826 1107 72-73 0.2946817215409254 1107 74-75 0.22053703429551064 1107 76-77 0.16779787294843374 1107 78-79 0.16884775797327478 1107 80-81 0.166446143433987 1107 82-83 0.21011557422983884 1107 84-85 0.20242555713414845 1107 86-87 0.20789249761352835 1107 88-89 0.18806378580264038 1107 90-91 0.2911940518423748 1107 92-93 0.19596494348462556 1107 94-95 0.16205204455253863 1107 96-97 0.16516348352590882 1107 98-99 0.1739732742772233 1107 100 0.2117453550972037 1108 1 0.17783385389644835 1108 2 0.3584193310422812 1108 3 0.23905833328559112 1108 4 0.15500650462219312 1108 5 0.13914704372420061 1108 6 0.1582429459989214 1108 7 0.16479676475368876 1108 8 0.16616215762372377 1108 9 0.18255885389429238 1108 10-11 0.20145472151720867 1108 12-13 0.20950763086468527 1108 14-15 0.21468313365925695 1108 16-17 0.24601375851469243 1108 18-19 0.2264883845519421 1108 20-21 0.26184764430760765 1108 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-0.8393540908166912 2215 24-25 -0.8562604733947197 2215 26-27 -0.8565946258425257 2215 28-29 -0.9182765081878514 2215 30-31 -0.8899415356149518 2215 32-33 -0.8567093309547218 2215 34-35 -0.9434789755404225 2215 36-37 -0.8722500256579409 2215 38-39 -0.9890152058186601 2215 40-41 -0.9873374246742941 2215 42-43 -0.9135316042079538 2215 44-45 -0.978966058742202 2215 46-47 -0.9994916817531632 2215 48-49 -0.8967726807645633 2215 50-51 -0.8148594451360864 2215 52-53 -0.8508020099708986 2215 54-55 -0.8487096033731234 2215 56-57 -0.8321133951148951 2215 58-59 -0.8864031086851867 2215 60-61 -0.8628184277657667 2215 62-63 -0.7827918222105978 2215 64-65 -0.774997932803764 2215 66-67 -0.7710872633580266 2215 68-69 -0.7421376413283696 2215 70-71 -0.7090261506859363 2215 72-73 -0.7013982954351548 2215 74-75 -0.663743725692818 2215 76-77 -0.7111994796126879 2215 78-79 -0.7566849172281991 2215 80-81 -0.7364352900387985 2215 82-83 -0.6794757249122227 2215 84-85 -0.6870539063547128 2215 86-87 -0.7546518230543668 2215 88-89 -0.6509941212427357 2215 90-91 -0.658104471706423 2215 92-93 -0.7032638537328495 2215 94-95 -0.7111309569575752 2215 96-97 -0.6928864114985771 2215 98-99 -0.6977717457274615 2215 100 -0.8619858837550787 2216 1 -0.4562213793509926 2216 2 -0.26947297509501666 2216 3 -0.495595880487798 2216 4 -0.38942363275914715 2216 5 -0.5006257134879775 2216 6 -0.5295801784624672 2216 7 -0.5076781543738846 2216 8 -0.559773450053612 2216 9 -0.5998173512881593 2216 10-11 -0.7392550205868034 2216 12-13 -0.6218036402108851 2216 14-15 -0.8432778607518898 2216 16-17 -0.9239485704771795 2216 18-19 -0.9881884107489611 2216 20-21 -0.8527579430156891 2216 22-23 -0.906886248100399 2216 24-25 -0.9622420694384246 2216 26-27 -0.9569504811673184 2216 28-29 -0.9572725018422332 2216 30-31 -0.9589676440820583 2216 32-33 -1.0183570000558078 2216 34-35 -1.0227129920415123 2216 36-37 -1.0302344842635165 2216 38-39 -1.0853480655055563 2216 40-41 -1.0481352190124653 2216 42-43 -1.1449095430393115 2216 44-45 -0.9503243071190894 2216 46-47 -1.028040979246562 2216 48-49 -1.1036523435448515 2216 50-51 -1.1172325378818542 2216 52-53 -1.0952677926354326 2216 54-55 -1.006401497400418 2216 56-57 -0.9142470999194146 2216 58-59 -1.0116807296149801 2216 60-61 -1.0725794741727768 2216 62-63 -1.0809074746740777 2216 64-65 -0.9722133467893599 2216 66-67 -0.9350026440435926 2216 68-69 -0.9950967939769697 2216 70-71 -1.0267370112539353 2216 72-73 -0.9889732463641465 2216 74-75 -1.0779765942735153 2216 76-77 -0.90179159970792 2216 78-79 -0.9149332941101136 2216 80-81 -0.862597843841101 2216 82-83 -0.8474365425624839 2216 84-85 -0.9003912936051393 2216 86-87 -0.802983718901789 2216 88-89 -0.8290523983693348 2216 90-91 -0.9692833549349942 2216 92-93 -0.8125261235541288 2216 94-95 -0.7832016398978254 2216 96-97 -0.8530788070946969 2216 98-99 -0.8466184476797523 2216 100 -0.9431372375772398 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 13.0 13 50.0 14 229.0 15 661.0 16 1600.0 17 3326.0 18 5879.0 19 9204.0 20 13618.0 21 19285.0 22 26344.0 23 30951.0 24 34426.0 25 38413.0 26 46135.0 27 58071.0 28 74450.0 29 93448.0 30 115276.0 31 142744.0 32 176350.0 33 221716.0 34 301907.0 35 411078.0 36 610961.0 37 1015542.0 38 865410.0 39 321175.0 40 622.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.87938120614051 21.804370571739465 13.522710833333873 24.793537388786156 2 14.946591752047933 20.314579312363822 40.123349134445554 24.61547980114269 3 18.072407072431 26.519395852505852 29.671934622761352 25.73626245230179 4 12.301643690818711 17.280931250136117 39.19969828903229 31.217726770012877 5 12.620128775460401 38.91350986856004 35.30636987547566 13.15999148050389 6 29.593801271727465 39.16558001951761 17.78711143427292 13.453507274482005 7 26.032352706585872 32.20323052812431 24.13486474076661 17.629552024523214 8 25.977006984649552 35.43699167494272 19.679936157099753 18.90606518330798 9 25.96568961477182 14.5440274790052 21.63411180311139 37.856171103111585 10-11 25.63194576630127 25.938840502042837 27.46849621471702 20.96071751693887 12-13 25.89968240226589 23.636434774117156 27.914098791369568 22.54978403224739 14-15 23.19564036339647 24.745217162592755 25.460314414697983 26.59882805931279 16-17 21.886930787581267 27.221349618236356 26.86209211392591 24.029627480256465 18-19 21.286539206495007 27.568832782518736 29.08614717280244 22.058480838183815 20-21 22.86760472962741 26.79804233150398 28.202668507338668 22.131684431529944 22-23 23.30443319212727 26.32743653757065 27.920644936123136 22.447485334178946 24-25 22.814362583093747 26.28499034358888 28.763947041607178 22.136700031710195 26-27 22.756230546216013 26.612172897805696 27.619258876982993 23.012337678995294 28-29 21.859025667148178 27.2289236620767 28.279639059972787 22.632411610802333 30-31 22.78181167163589 26.810806126555786 28.12444019438283 22.28294200742549 32-33 23.21799388517116 26.900730714069994 27.34249616341161 22.538779237347235 34-35 23.072669715544645 26.771279181437702 28.15420366835422 22.001847434663436 36-37 22.811172162632975 26.295736455748976 27.81785803361884 23.07523334799921 38-39 23.002381821329124 26.297590348719424 28.521464308141155 22.178563521810297 40-41 22.509757772697032 25.6158617213776 29.06480808025065 22.809572425674723 42-43 22.737212001720685 25.77100172316359 28.157379040911312 23.334407234204416 44-45 22.85813385840181 26.70342045408737 27.887346684668113 22.551099002842708 46-47 22.89119135689229 26.500828754828476 27.1837511885933 23.424228699685937 48-49 23.752877593390963 26.207349695259115 27.35852717230681 22.68124553904311 50-51 23.14890403667949 26.203322046861672 27.1274122434972 23.52036167296164 52-53 22.504805570819034 26.731358190014117 27.032400228761773 23.731436010405083 54-55 22.604378351705986 26.68760424146489 26.8522672228942 23.85575018393492 56-57 23.72339108037142 26.06815888700757 26.645959807337178 23.562490225283835 58-59 22.388447887520993 26.634930520323408 27.63439709351913 23.34222449863647 60-61 22.639717362994496 27.197301591784058 26.821349018718514 23.341632026502932 62-63 23.203626214650193 26.372209540779934 26.33328856684804 24.090875677721833 64-65 22.2200174309053 26.563904229613698 26.894586998993507 24.32149134048749 66-67 22.925887179403162 26.19493641470465 26.88486483934616 23.994311566546028 68-69 22.367768237387324 26.123662387521122 27.26118337752385 24.247385997567697 70-71 22.270288529702373 25.83100130603589 27.339565989088022 24.55914417517372 72-73 22.918978194553997 25.861041667969065 27.723923863633672 23.496056273843273 74-75 23.02613993032801 26.319316300486324 27.204314403476705 23.450229365708964 76-77 22.71919059002483 26.265899532566735 26.687964849052666 24.32694502835578 78-79 22.53710904343468 26.35521100269732 26.861951609904366 24.24572834396363 80-81 22.067620169747276 26.84053217703722 27.528372862381527 23.56347479083398 82-83 23.034226390490563 25.683594918387897 28.441396579958607 22.840782111162934 84-85 23.359794248375454 26.17858503719601 26.941918390291043 23.519702324137494 86-87 21.814389823216853 26.87840918559627 27.51233459154018 23.794866399646693 88-89 22.83608280127416 25.643626225570383 27.35779977313804 24.16249120001742 90-91 22.38396039121565 25.68834297316391 27.515609885553015 24.412086750067424 92-93 22.744091658362873 26.01523313215946 27.193333451189 24.047341758288667 94-95 21.550557815714726 25.978451791925856 27.944819013850108 24.526171378509307 96-97 21.94782045189162 25.86355553596176 27.458554001573514 24.730070010573105 98-99 21.32214894878814 26.540213404834752 27.363671854874195 24.77396579150291 100 22.190121149606266 25.748579345947896 26.583485310612936 25.4778141938329 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 47.0 2 36.5 3 53.0 4 37.0 5 31.0 6 66.0 7 162.5 8 290.0 9 345.5 10 304.5 11 256.5 12 268.5 13 313.5 14 401.5 15 598.0 16 824.5 17 1127.0 18 1553.0 19 2084.0 20 2777.5 21 3471.0 22 4272.5 23 5118.5 24 6060.5 25 7268.0 26 8959.0 27 10967.0 28 14386.5 29 22392.5 30 33966.5 31 45251.0 32 60934.0 33 76542.0 34 88678.0 35 102376.5 36 115501.0 37 138431.0 38 149860.5 39 141043.5 40 131810.0 41 123614.0 42 123072.0 43 131656.0 44 144665.0 45 172929.5 46 201201.0 47 220312.0 48 231684.0 49 224960.5 50 212675.0 51 214607.0 52 216431.5 53 206359.0 54 195760.0 55 168174.5 56 144211.0 57 118695.0 58 91630.5 59 88730.5 60 73470.0 61 48238.0 62 34681.5 63 28218.0 64 20106.0 65 8739.0 66 4116.0 67 2812.0 68 2097.0 69 1601.5 70 1230.5 71 886.5 72 661.5 73 562.0 74 444.0 75 299.5 76 188.5 77 112.5 78 55.5 79 25.5 80 17.0 81 19.5 82 16.0 83 6.5 84 4.0 85 3.5 86 2.5 87 2.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013753299013319358 2 0.003966468680957307 3 0.001853892970447437 4 0.028800011726950882 5 0.002479042925598317 6 0.0 7 3.664672150884469E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.009204794167221577 16-17 0.003298204935796022 18-19 0.0 20-21 3.2335342507804136E-5 22-23 8.622758002081102E-4 24-25 0.0 26-27 1.7245516004162206E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 4.7425169011446066E-4 36-37 0.0 38-39 0.0 40-41 4.5269479510925785E-4 42-43 7.652697726846979E-4 44-45 0.0 46-47 0.0 48-49 6.035930601456771E-4 50-51 0.0 52-53 0.0 54-55 3.880241100936496E-4 56-57 1.1856292252861517E-4 58-59 2.694611875650345E-4 60-61 8.622758002081103E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.001541317992871997 70-71 8.730542477107117E-4 72-73 0.0 74-75 1.5089826503641928E-4 76-77 0.0032335342507804135 78-79 5.281439276274676E-4 80-81 0.0036107799133714615 82-83 0.0018000007329344301 84-85 0.006132936628980184 86-87 0.008784434714620124 88-89 0.008267069234495257 90-91 0.0068982064016648815 92-93 0.009592818277315226 94-95 0.0017029947054110178 96-97 0.004968864298699235 98-99 0.0021125757105098703 100 0.08351141125015549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4638887.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.96545541766627 #Duplication Level Percentage of deduplicated Percentage of total 1 65.71383221794133 14.434342519071919 2 16.276325484012176 7.150338035649896 3 6.705188180268011 4.418475361222194 4 3.473456191050213 3.0518418843892143 5 1.9932833404286217 2.189168817448089 6 1.249150169833224 1.6462891419265158 7 0.8419670303439988 1.2945932487716179 8 0.5893639809014695 1.0356518597815634 9 0.42140259935294955 0.8330670008078306 >10 2.0552928748280155 8.193516748245653 >50 0.24113293547292158 3.781401295123723 >100 0.3176341304835165 15.777178745188813 >500 0.066092103221654 10.244690375021175 >1k 0.052736195289771165 20.62027491667355 >5k 0.0025533353399144324 3.5105004413980456 >10k+ 5.892312322879459E-4 1.8186696092802581 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 20304 0.4376911961856368 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 14897 0.3211330648925055 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13033 0.2809510126028075 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 12409 0.26749951011956097 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 12060 0.25997615376274524 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 11607 0.2502108803253884 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 9443 0.2035617595341296 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8979 0.19355936025171555 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 7475 0.1611377901638906 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7229 0.15583479399261074 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6815 0.14691023946045678 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6700 0.14443119653485847 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6695 0.14432341205983246 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 6668 0.143741375894692 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 6575 0.14173658465920813 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6501 0.14014137442882313 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6496 0.1400335899537971 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6311 0.1360455643778346 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5984 0.1289964597111333 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5865 0.12643118920551416 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5842 0.1259353806203945 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5837 0.1258275961453685 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5754 0.12403837385993666 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5645 0.12168867230436956 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5543 0.11948986901383887 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5472 0.11795932946846949 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5296 0.1141653159475538 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 5222 0.1125701057171688 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5111 0.11017729037159128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5106 0.11006950589656528 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 5098 0.10989705073652366 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 5078 0.1094659128364196 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4930 0.1062754923756496 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 4906 0.10575812689552473 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 4872 0.10502519246534782 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4859 0.1047449528302802 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 4815 0.10379644945005127 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 4785 0.10314974259989519 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 4758 0.1025677064347547 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4758 0.1025677064347547 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.467068501560827E-5 2 0.0 0.0 0.0 0.0 6.467068501560827E-5 3 0.0 0.0 0.0 0.0 6.467068501560827E-5 4 0.0 0.0 0.0 0.0 6.467068501560827E-5 5 0.0 0.0 0.0 0.0 1.508982650364193E-4 6 0.0 0.0 0.0 0.0 1.508982650364193E-4 7 0.0 0.0 0.0 0.0 1.508982650364193E-4 8 0.0 0.0 0.0 0.0 1.508982650364193E-4 9 0.0 0.0 0.0 0.0 1.508982650364193E-4 10-11 0.0 0.0 0.0 0.0 1.508982650364193E-4 12-13 0.0 0.0 0.0 0.0 1.8323360754422344E-4 14-15 0.0 0.0 0.0 0.0 2.2634739755462895E-4 16-17 0.0 0.0 0.0 0.0 2.694611875650345E-4 18-19 0.0 0.0 0.0 0.0 3.2335342507804136E-4 20-21 0.0 0.0 0.0 2.1556895005202757E-5 3.2335342507804136E-4 22-23 0.0 0.0 0.0 7.544913251820965E-5 3.2335342507804136E-4 24-25 0.0 0.0 0.0 1.9401205504682482E-4 3.2335342507804136E-4 26-27 0.0 0.0 0.0 3.341318725806427E-4 4.095810050988524E-4 28-29 0.0 0.0 0.0 0.0013796412803329765 4.3113790010405515E-4 30-31 0.0 0.0 0.0 0.006779643479136267 4.4191634760665656E-4 32-33 0.0 0.0 0.0 0.01868982796951079 4.526947951092579E-4 34-35 0.0 0.0 0.0 0.03760600333657621 4.958085851196634E-4 36-37 0.0 0.0 0.0 0.0711485319646717 4.958085851196634E-4 38-39 0.0 0.0 0.0 0.12730424345322489 4.958085851196634E-4 40-41 0.0 0.0 0.0 0.19165157504375513 4.958085851196634E-4 42-43 0.0 0.0 0.0 0.25837016508485766 5.065870326222648E-4 44-45 0.0 0.0 0.0 0.341019300534805 5.281439276274675E-4 46-47 0.0 0.0 0.0 0.44595826542013206 5.389223751300689E-4 48-49 0.0 0.0 0.0 0.5670217877693507 5.497008226326703E-4 50-51 0.0 0.0 0.0 0.6982062723235121 5.604792701352717E-4 52-53 0.0 0.0 0.0 0.8305656076554571 6.467068501560827E-4 54-55 0.0 0.0 0.0 0.9765057868406797 6.467068501560827E-4 56-57 0.0 0.0 0.0 1.1598148435174211 6.467068501560827E-4 58-59 0.0 0.0 0.0 1.3809131371382835 6.467068501560827E-4 60-61 0.0 0.0 0.0 1.6108928715012891 6.467068501560827E-4 62-63 0.0 0.0 0.0 1.8579025529184048 7.329344301768938E-4 64-65 0.0 0.0 0.0 2.1237314036750625 7.544913251820965E-4 66-67 0.0 0.0 0.0 2.425700388907943 7.544913251820965E-4 68-69 0.0 0.0 0.0 2.76457477838973 7.544913251820965E-4 70-71 0.0 0.0 0.0 3.142758165913505 7.652697726846979E-4 72-73 0.0 0.0 0.0 3.5385643151040327 8.191620101977048E-4 74-75 0.0 0.0 0.0 3.9292830370733327 8.838326952133131E-4 76-77 0.0 0.0 0.0 4.382064059762611 9.053895902185158E-4 78-79 0.0 0.0 0.0 4.869260665327696 9.053895902185158E-4 80-81 0.0 0.0 0.0 5.401737528851209 9.053895902185158E-4 82-83 0.0 0.0 0.0 5.954844340894701 9.053895902185158E-4 84-85 0.0 0.0 0.0 6.497054142513064 9.161680377211172E-4 86-87 0.0 0.0 0.0 7.078713061990948 9.377249327263199E-4 88 0.0 0.0 0.0 7.546486905156344 9.485033802289213E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8100 0.0 37.56268 1 GGGCACC 4700 0.0 37.130074 94 GTATCAA 13080 0.0 35.70087 1 CAACGCA 13850 0.0 31.523977 5 GTACTAG 5410 0.0 31.20574 1 ATCAACG 14295 0.0 30.584423 3 TATTCTC 2595 0.0 28.433964 5 CTGTGCG 6850 0.0 27.994804 9 GTGGTAT 2025 0.0 27.867212 1 TATCAAC 16700 0.0 27.75812 2 AGGGCTA 5580 0.0 27.710016 5 AACGCAG 16745 0.0 27.308222 6 CTATTGA 5635 0.0 26.690945 9 GCTATTG 5635 0.0 26.521553 8 TACCCCG 390 0.0 26.51143 5 TAGTACT 7300 0.0 26.016888 4 ACTGTGC 7695 0.0 25.834345 8 ACGCAGA 17150 0.0 25.26577 7 GTACTGT 7680 0.0 25.089289 6 GCATCGT 6410 0.0 25.074587 8 >>END_MODULE