FastQCFastQC Report
Mon 16 Jan 2017
ERR1146449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510331
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC150120.33283588277667425No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN137750.3054099577170722No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT128750.28545576810216367No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA106680.2365236609020491No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC99030.21956259972937686No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC86210.19113896518902937No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA85650.18989737116854616No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC69770.1546893121591298No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG66360.14712889142725888No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT65100.1443353048811717No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC64380.14273896971197902No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT57540.12757378560464852No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT57500.12748510031747115No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG53330.1182396591292302No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53270.11810663119846414No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT52550.11651029602927146No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG51300.11373888080497861No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC49700.11019146931788378No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA47680.10571286231542652No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG47350.10498120869621319No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA47280.1048260094436528No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC44850.035.78038894
GTACTAG45050.033.397051
CAACGCA81750.032.465365
GTATCAA90350.032.312811
GCATCGT44500.030.8066848
GGTATCA59100.030.4053271
ATCAACG89200.029.759163
TAGGCAT57450.029.1084755
CTAGGCA64550.027.073234
CTGTGCG64450.026.809029
CTAACGC23600.026.6765613
TATCAAC109200.026.546622
AACGCAG107550.026.3199736
GTACATG182100.026.185051
TAGTACT66850.026.001224
TCTAACG24050.025.7872812
ACGCAGA101250.025.780847
TATTCTC23150.025.5669575
CGCAGAG101550.025.5662678
CAGTACG7600.025.3294819
Produced by FastQC (version 0.11.5)