##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146449_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4510331 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.152409656852235 31.0 30.0 34.0 26.0 34.0 2 30.249702516289826 31.0 30.0 34.0 26.0 34.0 3 30.431201169049455 31.0 30.0 34.0 26.0 34.0 4 33.9174714671717 35.0 35.0 37.0 28.0 37.0 5 34.137151796619804 35.0 35.0 37.0 30.0 37.0 6 34.3735561758106 37.0 35.0 37.0 30.0 37.0 7 34.2656652028421 37.0 35.0 37.0 30.0 37.0 8 34.2043659323451 37.0 35.0 37.0 30.0 37.0 9 35.579801571104205 39.0 35.0 39.0 29.0 39.0 10-11 35.499443943249396 39.0 35.0 39.0 28.5 39.0 12-13 35.48347660071955 39.0 35.0 39.0 29.0 39.0 14-15 36.46700308691314 40.0 36.0 41.0 27.5 41.0 16-17 36.287106533866364 39.5 35.5 41.0 27.0 41.0 18-19 36.22717988990165 39.0 35.0 41.0 27.0 41.0 20-21 36.55814927995307 39.5 36.0 41.0 28.5 41.0 22-23 36.272980297898314 39.0 35.5 41.0 27.0 41.0 24-25 36.363582074131585 39.0 35.0 41.0 27.5 41.0 26-27 36.42599345812979 39.0 35.0 41.0 28.5 41.0 28-29 36.26004111449913 39.0 35.0 41.0 27.5 41.0 30-31 36.19747153811993 39.0 35.0 41.0 27.5 41.0 32-33 36.29688153707566 39.0 35.0 41.0 28.0 41.0 34-35 36.34214384709237 39.0 35.0 41.0 28.5 41.0 36-37 36.266066614623185 39.0 35.0 41.0 28.0 41.0 38-39 36.10914354179327 39.0 35.0 41.0 28.0 41.0 40-41 36.06930322852136 39.0 35.0 41.0 28.0 41.0 42-43 35.9013341814603 39.0 34.0 41.0 27.0 41.0 44-45 35.718508353378056 38.5 34.0 40.5 27.0 41.0 46-47 35.594079237200106 38.0 34.0 40.0 26.5 41.0 48-49 35.46171245524996 38.0 34.0 40.0 26.5 41.0 50-51 35.24973998582365 38.0 33.5 40.0 26.0 41.0 52-53 35.064666872564345 38.0 33.0 40.0 26.0 41.0 54-55 34.86892026328001 37.0 33.0 40.0 26.0 41.0 56-57 34.71636848825507 37.0 33.0 40.0 26.0 41.0 58-59 34.45753670850321 36.5 33.0 40.0 26.0 41.0 60-61 34.20923963230193 36.0 32.5 39.5 25.5 41.0 62-63 33.93554275728322 35.5 32.0 39.0 25.0 41.0 64-65 33.646039614387504 35.0 32.0 39.0 25.0 41.0 66-67 33.35965730674755 35.0 32.0 38.5 24.5 40.0 68-69 33.07131704967995 35.0 31.0 38.0 24.0 40.0 70-71 33.0095007439587 35.0 31.0 37.0 24.0 40.0 72-73 32.79935918228618 35.0 31.0 37.0 24.0 40.0 74-75 32.53938036920128 35.0 31.0 36.0 24.0 39.0 76-77 32.31337744391709 35.0 31.0 36.0 24.0 39.0 78-79 32.09607620815412 35.0 31.0 36.0 23.0 39.0 80-81 31.878253059476123 35.0 31.0 35.0 23.0 37.5 82-83 31.65265609109398 34.5 31.0 35.0 23.0 37.0 84-85 31.474325276792325 34.0 30.5 35.0 23.0 36.5 86-87 31.323350104460182 34.0 30.5 35.0 23.0 36.0 88-89 31.339919841803184 34.0 31.0 35.0 23.0 36.0 90-91 31.26672332917473 34.0 31.0 35.0 23.0 36.0 92-93 31.13414336996553 34.0 31.0 35.0 22.5 35.5 94-95 31.118233894585565 34.0 31.0 35.0 23.0 35.0 96-97 31.035779413972058 34.0 31.0 35.0 21.5 35.0 98-99 30.966928812985124 34.0 30.5 35.0 20.5 35.0 100 30.45899003864683 34.0 29.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8855247025682083 1101 2 1.1067386839174986 1101 3 1.0399241543538444 1101 4 1.1756877735362252 1101 5 1.0632045385638023 1101 6 0.811763328942817 1101 7 0.8634466988582048 1101 8 0.9330740220626765 1101 9 1.1440240535855253 1101 10-11 1.1989323544178703 1101 12-13 1.0731644093616168 1101 14-15 1.3507616422332163 1101 16-17 1.4443686796770194 1101 18-19 1.621805149902663 1101 20-21 1.2655945523612928 1101 22-23 1.5869541293664255 1101 24-25 1.4107633380945188 1101 26-27 1.309926647349478 1101 28-29 1.489887753784032 1101 30-31 1.5361987629457516 1101 32-33 1.3693765193235308 1101 34-35 1.3035120137039016 1101 36-37 1.2670678482275335 1101 38-39 1.3082313552627696 1101 40-41 1.2333192530545958 1101 42-43 1.2467098782833403 1101 44-45 1.273728898814646 1101 46-47 1.287593247212122 1101 48-49 1.2822446779366246 1101 50-51 1.317117597595015 1101 52-53 1.2748166135269798 1101 54-55 1.2662011472205563 1101 56-57 1.1021417632684134 1101 58-59 1.0573778918388328 1101 60-61 1.1387024153524905 1101 62-63 1.1137247753794384 1101 64-65 1.1412515562913441 1101 66-67 1.1189655642169427 1101 68-69 1.1083419396912149 1101 70-71 1.0674490911617553 1101 72-73 0.983731506239053 1101 74-75 1.0210224125877474 1101 76-77 0.9716487450823834 1101 78-79 0.9443426484420172 1101 80-81 0.9406343050975359 1101 82-83 0.9721464681781669 1101 84-85 1.02938273690744 1101 86-87 1.1067676957706887 1101 88-89 1.0822426775959961 1101 90-91 1.0631401334606814 1101 92-93 1.112057973056281 1101 94-95 1.105849922666998 1101 96-97 1.0253174653449868 1101 98-99 1.0564930044247909 1101 100 1.1208813345301323 1102 1 0.8955960397289218 1102 2 1.0719724923012066 1102 3 1.0589135773837306 1102 4 1.1497572048218103 1102 5 0.9703368000450112 1102 6 0.779899370621969 1102 7 0.8287342402416584 1102 8 0.8292838663245448 1102 9 0.9876628696966563 1102 10-11 1.071403628792389 1102 12-13 1.0134023680315707 1102 14-15 1.2182357114473987 1102 16-17 1.4167924519087336 1102 18-19 1.4115659052591027 1102 20-21 1.156341776843803 1102 22-23 1.4203942954820832 1102 24-25 1.306563527032047 1102 26-27 1.157613096154705 1102 28-29 1.3539958395403033 1102 30-31 1.4060111946125673 1102 32-33 1.2541398777452955 1102 34-35 1.1436837668050686 1102 36-37 1.1404894368369796 1102 38-39 1.2088385128312211 1102 40-41 1.1012661907584729 1102 42-43 1.1521297938968402 1102 44-45 1.2634073865965263 1102 46-47 1.220619676466498 1102 48-49 1.140108645375804 1102 50-51 1.2199858832134396 1102 52-53 1.1656203390728521 1102 54-55 1.2308829711523117 1102 56-57 1.1161465007459768 1102 58-59 1.066337806224979 1102 60-61 1.0731179240639648 1102 62-63 1.070260676549509 1102 64-65 1.0125316044361128 1102 66-67 1.059133344332686 1102 68-69 1.1207969776538462 1102 70-71 1.0032032209679471 1102 72-73 0.8716011386199369 1102 74-75 0.9078758580562507 1102 76-77 0.849423991197618 1102 78-79 0.8969900353465405 1102 80-81 1.0155522727179225 1102 82-83 1.0101176975399895 1102 84-85 0.9777635969885914 1102 86-87 0.9881341878286243 1102 88-89 0.9931039911677395 1102 90-91 0.959376880406257 1102 92-93 0.9798135706236764 1102 94-95 0.9865579593290335 1102 96-97 1.0063221443494719 1102 98-99 1.0047429332873676 1102 100 1.0601964424983628 1103 1 0.5770928733678389 1103 2 0.8056736381811511 1103 3 0.7484247285452241 1103 4 0.7952356086361405 1103 5 0.7588068918406634 1103 6 0.4697091946207834 1103 7 0.5787650773733546 1103 8 0.6792854576818712 1103 9 0.7689896371043048 1103 10-11 0.8316433073734046 1103 12-13 0.6916277065030059 1103 14-15 0.8286106334012189 1103 16-17 1.082785899256585 1103 18-19 1.1734060071411676 1103 20-21 0.8537228775174697 1103 22-23 1.0917700689954088 1103 24-25 0.974288675493959 1103 26-27 0.8645244197107971 1103 28-29 1.1403013186936022 1103 30-31 1.119620775845057 1103 32-33 0.9184988471118842 1103 34-35 0.8914082908242307 1103 36-37 0.8342714207359663 1103 38-39 0.9508743010642462 1103 40-41 0.8420224946968702 1103 42-43 0.874851866712369 1103 44-45 0.9858771614407331 1103 46-47 0.9176111420977975 1103 48-49 0.8974620820989614 1103 50-51 0.9088462095604939 1103 52-53 0.8853058435969317 1103 54-55 0.9480368424993628 1103 56-57 0.8204073343326783 1103 58-59 0.8110505398263257 1103 60-61 0.8311802481438164 1103 62-63 0.7830976427976211 1103 64-65 0.7812219862870649 1103 66-67 0.8541323462509709 1103 68-69 0.8292306783340564 1103 70-71 0.7426187421873465 1103 72-73 0.7508268065096786 1103 74-75 0.7252274262178915 1103 76-77 0.7217129361482435 1103 78-79 0.7491476683836495 1103 80-81 0.7756199820389789 1103 82-83 0.6765387580029127 1103 84-85 0.6942459082085115 1103 86-87 0.7788124987575493 1103 88-89 0.7100985342787887 1103 90-91 0.7455034326946652 1103 92-93 0.8327466328542599 1103 94-95 0.8138829287229079 1103 96-97 0.7975369608689071 1103 98-99 0.8377249854900484 1103 100 0.9065615440174284 1104 1 0.5381325283465088 1104 2 0.7881651048259322 1104 3 0.7426360778716337 1104 4 0.8356717083454512 1104 5 0.7746888672356675 1104 6 0.4976787118880921 1104 7 0.45885528367082173 1104 8 0.503888891451318 1104 9 0.7283819852775437 1104 10-11 0.6728445609362339 1104 12-13 0.5714016361018608 1104 14-15 0.8452757529886625 1104 16-17 0.970480649710062 1104 18-19 0.9142155250928425 1104 20-21 0.7799999116646035 1104 22-23 1.0769360119036833 1104 24-25 0.8907018508887248 1104 26-27 0.7532115026252839 1104 28-29 0.9738606875695268 1104 30-31 0.9605259565046751 1104 32-33 0.8133566236626706 1104 34-35 0.6983175047785224 1104 36-37 0.686778633223021 1104 38-39 0.8574837856625592 1104 40-41 0.7581979637085396 1104 42-43 0.699508151836902 1104 44-45 0.7669571671033353 1104 46-47 0.7661638704194402 1104 48-49 0.7018976105960633 1104 50-51 0.8558846066011654 1104 52-53 0.7806549225015544 1104 54-55 0.7424234724534386 1104 56-57 0.7234115638822303 1104 58-59 0.7440644265499046 1104 60-61 0.685552196118941 1104 62-63 0.6648633414422846 1104 64-65 0.6508309586863774 1104 66-67 0.7085660880932778 1104 68-69 0.736160504409213 1104 70-71 0.5538487599685311 1104 72-73 0.5856709871163446 1104 74-75 0.5425920534904733 1104 76-77 0.6412930172124831 1104 78-79 0.5368168088866554 1104 80-81 0.5606946946951901 1104 82-83 0.5655165649419871 1104 84-85 0.6252893659719696 1104 86-87 0.5271026405580805 1104 88-89 0.5160404973013542 1104 90-91 0.5037968736170093 1104 92-93 0.5767467522848371 1104 94-95 0.5162548321332849 1104 96-97 0.5367812712972011 1104 98-99 0.5835214043243511 1104 100 0.5902562441415569 1105 1 0.5288792267591234 1105 2 0.6639776034329969 1105 3 0.5908456575764554 1105 4 0.7355164301906711 1105 5 0.6334544525776593 1105 6 0.32158326969521056 1105 7 0.43775419881495736 1105 8 0.47858026716410507 1105 9 0.5234214057692483 1105 10-11 0.5928728379283186 1105 12-13 0.5275478043733344 1105 14-15 0.7005266134260211 1105 16-17 0.8168348005478876 1105 18-19 0.8728914305890143 1105 20-21 0.665057281873942 1105 22-23 0.9165088186096924 1105 24-25 0.7439334223624101 1105 26-27 0.6255382023924909 1105 28-29 0.8970675935580417 1105 30-31 0.9149199180014236 1105 32-33 0.7790872327432012 1105 34-35 0.6288882379252811 1105 36-37 0.5799209742088962 1105 38-39 0.7316042451568094 1105 40-41 0.609003110500268 1105 42-43 0.6027449477305353 1105 44-45 0.6498671523736519 1105 46-47 0.5614779709407571 1105 48-49 0.6925307032008661 1105 50-51 0.7294527211702331 1105 52-53 0.7298730902265689 1105 54-55 0.666676962901434 1105 56-57 0.5499247317360485 1105 58-59 0.6187648183669268 1105 60-61 0.6441785528400175 1105 62-63 0.47899052755811766 1105 64-65 0.4558619737955141 1105 66-67 0.5425979886041148 1105 68-69 0.5309151144171125 1105 70-71 0.5489434131437463 1105 72-73 0.4705599566147072 1105 74-75 0.3899224906716725 1105 76-77 0.5018293335883044 1105 78-79 0.5578114697031324 1105 80-81 0.5523324750953016 1105 82-83 0.525174943594628 1105 84-85 0.44447834206960124 1105 86-87 0.514824385346472 1105 88-89 0.5359051964022612 1105 90-91 0.5892829465580185 1105 92-93 0.6083214423870054 1105 94-95 0.5176245419015579 1105 96-97 0.5511205855059735 1105 98-99 0.6443144408816863 1105 100 0.7315089659775182 1106 1 0.42360061777865 1106 2 0.5080865178459781 1106 3 0.49504003704916144 1106 4 0.6257402886844048 1106 5 0.5289586623107851 1106 6 0.2456273865949754 1106 7 0.31871341398538533 1106 8 0.3779112397365978 1106 9 0.5607270168593104 1106 10-11 0.4843672387436939 1106 12-13 0.4209968745152253 1106 14-15 0.6545410683675783 1106 16-17 0.7539385273849248 1106 18-19 0.700721058718365 1106 20-21 0.5331629455221503 1106 22-23 0.8200454771893746 1106 24-25 0.6512372621558953 1106 26-27 0.5297192426953004 1106 28-29 0.7690298380811811 1106 30-31 0.700105381464077 1106 32-33 0.5975657216530053 1106 34-35 0.5797785140878418 1106 36-37 0.5210743278395498 1106 38-39 0.559741545323341 1106 40-41 0.5677350581569982 1106 42-43 0.5358072872067865 1106 44-45 0.5549392129287654 1106 46-47 0.5413044274329195 1106 48-49 0.5578260669046742 1106 50-51 0.6536891943445227 1106 52-53 0.6071536345663233 1106 54-55 0.612358066361125 1106 56-57 0.5468872303386121 1106 58-59 0.5230637696722411 1106 60-61 0.5703866279197598 1106 62-63 0.5273540019708634 1106 64-65 0.5549027876627264 1106 66-67 0.5629980983664282 1106 68-69 0.48929008047936406 1106 70-71 0.5341169711704055 1106 72-73 0.42135164995971763 1106 74-75 0.5202263261095155 1106 76-77 0.5291718007140531 1106 78-79 0.5422702115598881 1106 80-81 0.48722886876451454 1106 82-83 0.40757520653054513 1106 84-85 0.493839804173291 1106 86-87 0.5240129863270724 1106 88-89 0.5154050882370207 1106 90-91 0.495743297100514 1106 92-93 0.5379452705363903 1106 94-95 0.44637733133069446 1106 96-97 0.45382869107524826 1106 98-99 0.4661635656143588 1106 100 0.4919163859679081 1107 1 0.5859324972475157 1107 2 0.7031236463054213 1107 3 0.6378467587057877 1107 4 0.6879016508865305 1107 5 0.5841074088413336 1107 6 0.44803566375627213 1107 7 0.46830136161648284 1107 8 0.5012890453134062 1107 9 0.581496801795339 1107 10-11 0.6273479215201476 1107 12-13 0.5112491879468095 1107 14-15 0.7587177175543616 1107 16-17 0.8561688411106303 1107 18-19 0.9767148895674751 1107 20-21 0.7440173189363435 1107 22-23 0.9998264295171424 1107 24-25 0.687257266288519 1107 26-27 0.6204392686079601 1107 28-29 0.8160225166356554 1107 30-31 0.8547677070143038 1107 32-33 0.7185369254564975 1107 34-35 0.6440597074853258 1107 36-37 0.6701984722282148 1107 38-39 0.6725923296841501 1107 40-41 0.4999497907743802 1107 42-43 0.6861228186119277 1107 44-45 0.6802022023049474 1107 46-47 0.6703935375971213 1107 48-49 0.6750352800493999 1107 50-51 0.7123975818952744 1107 52-53 0.7740524355469844 1107 54-55 0.7144701721562896 1107 56-57 0.5979564071087964 1107 58-59 0.5866657793932148 1107 60-61 0.7160019513906448 1107 62-63 0.6726345270746918 1107 64-65 0.7031409310349872 1107 66-67 0.713201192621888 1107 68-69 0.6931949445030199 1107 70-71 0.6843005192326359 1107 72-73 0.6192350764632408 1107 74-75 0.6200717250287013 1107 76-77 0.641606623467311 1107 78-79 0.5778737886993994 1107 80-81 0.7012742047635818 1107 82-83 0.6826387316585709 1107 84-85 0.6102701480559851 1107 86-87 0.6948632829638264 1107 88-89 0.7181294552661335 1107 90-91 0.6872470322849615 1107 92-93 0.7128804423288173 1107 94-95 0.6690296008298802 1107 96-97 0.6593052551895546 1107 98-99 0.7501131624973922 1107 100 0.7355017907323393 1108 1 0.24092495070549802 1108 2 0.37152907831350035 1108 3 0.3556316242368851 1108 4 0.546111796638634 1108 5 0.4343173563065861 1108 6 0.18139874011661306 1108 7 0.1867132341916431 1108 8 0.27752661999488026 1108 9 0.34271628843519863 1108 10-11 0.41563795659213554 1108 12-13 0.2780003706264509 1108 14-15 0.4554103455273051 1108 16-17 0.6305635686333275 1108 18-19 0.668926234804772 1108 20-21 0.26027028713647127 1108 22-23 0.6102574255067168 1108 24-25 0.4241012156667878 1108 26-27 0.3745563119257511 1108 28-29 0.5086907478155922 1108 30-31 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-0.6901120688266857 2216 3 -0.9062503063479106 2216 4 -0.9193041238503028 2216 5 -1.0303152447091506 2216 6 -0.9760545794872684 2216 7 -1.2082274474224448 2216 8 -1.0480912469551527 2216 9 -1.1810772928561235 2216 10-11 -1.2621251052121423 2216 12-13 -1.3651249993928403 2216 14-15 -1.8023307874693373 2216 16-17 -1.5433970331824796 2216 18-19 -3.4588295559136952 2216 20-21 -1.7150305887291424 2216 22-23 -1.2670265645866436 2216 24-25 -1.3777038578758791 2216 26-27 -1.4089288168872613 2216 28-29 -1.4032580934099883 2216 30-31 -1.587283161995373 2216 32-33 -1.4643111828534856 2216 34-35 -1.6364968483796218 2216 36-37 -1.642724372687674 2216 38-39 -1.8811653593107067 2216 40-41 -1.7942276374648785 2216 42-43 -1.7448567460311466 2216 44-45 -1.6297915567926182 2216 46-47 -1.8521136806954033 2216 48-49 -1.8305088801186855 2216 50-51 -1.685256257763399 2216 52-53 -1.67758163731272 2216 54-55 -1.5319261693588402 2216 56-57 -1.6235471003143545 2216 58-59 -1.6453637116982094 2216 60-61 -1.7010627141774393 2216 62-63 -1.650919623163979 2216 64-65 -1.6611974908957698 2216 66-67 -1.70722519364627 2216 68-69 -1.6984090368226035 2216 70-71 -1.795727731903547 2216 72-73 -1.6961037405361807 2216 74-75 -1.739872913951288 2216 76-77 -1.64489684668521 2216 78-79 -1.7677971507234886 2216 80-81 -1.7432938967708687 2216 82-83 -1.931114322926451 2216 84-85 -1.734707352051327 2216 86-87 -1.753402527557956 2216 88-89 -1.5674191927996652 2216 90-91 -1.6806393092635865 2216 92-93 -1.6110053265372528 2216 94-95 -1.4825296223268474 2216 96-97 -1.5844050949667121 2216 98-99 -1.5852413827071246 2216 100 -1.5192172748390362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18718.0 1 2792.0 2 494.0 3 456.0 4 385.0 5 234.0 6 239.0 7 321.0 8 520.0 9 999.0 10 1904.0 11 4120.0 12 7470.0 13 10362.0 14 13300.0 15 16212.0 16 18883.0 17 21679.0 18 24715.0 19 27359.0 20 31135.0 21 35004.0 22 39799.0 23 45569.0 24 51371.0 25 56873.0 26 65262.0 27 74358.0 28 87317.0 29 102655.0 30 121713.0 31 145570.0 32 177116.0 33 223409.0 34 303549.0 35 402119.0 36 588918.0 37 869561.0 38 703348.0 39 214367.0 40 156.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.573968094447 21.01704016554468 12.588073250072831 23.820918489935494 2 15.466211110658287 19.826351563368185 40.88641899260568 23.821018333367853 3 18.715320700880792 26.740522931583854 28.69522851161633 25.84892785591902 4 12.210035436246274 16.65249647452896 39.991549115697595 31.14591897352717 5 13.188872757717727 38.62563906158149 34.946269839856065 13.239218340844719 6 29.999913112891086 38.35234861425087 18.234574252052376 13.413164020805675 7 27.060293378397166 31.608968736964822 23.67313033216752 17.657607552470488 8 26.083836893349265 35.212582620015446 19.92662634027035 18.77695414636494 9 26.04650343907402 15.12523988162534 21.58240417159467 37.24585250770596 10-11 25.729220346175996 25.787015903696954 27.55141865470308 20.932345095423965 12-13 25.965398488421105 23.550679171847875 27.559490363121736 22.924431976609288 14-15 23.329588575520653 24.748265240293208 24.928018072904763 26.99412811128138 16-17 22.40916905801775 27.38651154284522 26.22039703504785 23.983922364089178 18-19 21.854980827732437 27.986930294121876 27.7674079141061 22.390680964039586 20-21 23.54981557382073 27.163360650859815 26.697441882183444 22.58938189313601 22-23 24.054487709718828 26.74099644593545 26.471380829217857 22.73313501512786 24-25 23.45325307579719 26.843288520374447 27.104525953574583 22.59893245025378 26-27 23.271780498223876 27.18513604545522 26.094225374427783 23.448858081893118 28-29 22.478510853964508 27.60351465634247 26.812363762051035 23.105610727641988 30-31 23.217659175558378 27.227849424288607 26.738694754069936 22.815796646083076 32-33 23.547176968063745 27.435122169513406 25.828893594697362 23.18880726772549 34-35 23.57240545557317 27.221508734516004 26.635225410603386 22.570860399307442 36-37 23.289859582698362 26.817959093774718 26.312327064326947 23.57985425919998 38-39 23.46961065871584 26.9244383497643 26.89037867757527 22.71557231394458 40-41 23.068231062382637 26.061415178627158 27.54818778098579 23.32216597800442 42-43 23.088835088124448 26.309439448393807 26.877661415853733 23.72406404762802 44-45 23.243895408523255 27.18344215738926 26.703593055324266 22.869069378763225 46-47 23.10553909368878 26.937028678127454 26.26790432361048 23.68952790457329 48-49 23.780682947702484 26.52289269938623 26.594343238942546 23.102081113968737 50-51 23.441102740971044 26.389713142063503 26.56767381395866 23.601510303006798 52-53 22.966068876364112 26.89865796680718 26.41606093616357 23.71921222066514 54-55 22.936944953471997 26.80967932267299 26.393181970668866 23.86019375318614 56-57 23.875336820591915 26.29295646572955 26.187770375834944 23.64393633784359 58-59 22.82415846315631 26.68176977390363 27.06836340358418 23.42570835935588 60-61 22.900116587444707 27.268356839154123 26.389014257724003 23.442512315677167 62-63 23.388884914445082 26.60594459417326 25.936677972659243 24.06849251872242 64-65 22.484182404764834 26.76798605428216 26.483691157447996 24.264140383505012 66-67 23.24123656469391 26.38151560873121 26.40410489855492 23.973142928019957 68-69 22.606268342186773 26.50710369089189 26.640214645036153 24.246413321885182 70-71 22.533597491206038 26.125216582299828 26.84771295666727 24.49347296982687 72-73 23.183366283755234 26.25551806722956 27.08379794375547 23.47731770525973 74-75 23.29795257137514 26.589345293374944 26.71838018305836 23.39432195219156 76-77 22.93875360521018 26.5032453299142 26.32667611489335 24.231324949982273 78-79 22.863575527336515 26.500871848473707 26.406001269176848 24.229551355012934 80-81 22.50197806410488 26.795915046882907 27.095644692881866 23.606462196130348 82-83 23.279178095350915 25.830986489960715 27.969083294059853 22.92075212062851 84-85 23.324872044177024 26.342951028394356 26.77007226868204 23.56210465874658 86-87 22.115055045077735 26.971786253520598 27.259370206235424 23.653788495166243 88-89 23.190976600812665 25.642146560179345 27.115202466022136 24.051674372985847 90-91 22.805290134387366 25.831194191831536 27.167412765000716 24.196102908780386 92-93 22.979345471434872 26.01575753445784 27.21529244778871 23.78960454631858 94-95 21.939239750389888 25.925552307315353 27.88986336094898 24.245344581345776 96-97 22.30806845636937 25.811761907905407 27.4133129681399 24.466856667585326 98-99 21.716583903062748 26.479055964812854 27.31748084094202 24.48687929118238 100 22.482744770534698 25.68856176760253 26.598179196179977 25.230514265682793 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15311.0 1 8978.5 2 2456.0 3 1743.5 4 891.0 5 455.0 6 346.5 7 317.5 8 306.5 9 305.5 10 277.5 11 262.5 12 267.0 13 277.0 14 287.0 15 335.0 16 392.0 17 457.5 18 569.0 19 718.5 20 924.0 21 1234.5 22 1678.5 23 2281.5 24 3160.5 25 4384.5 26 6256.0 27 8705.5 28 12257.0 29 19330.5 30 29737.5 31 40340.0 32 54109.5 33 69232.5 34 83337.0 35 97320.5 36 111037.0 37 133025.5 38 143290.0 39 137392.0 40 129979.5 41 122034.0 42 121922.0 43 130680.5 44 143487.5 45 167024.0 46 190239.5 47 206646.0 48 217547.0 49 213632.5 50 203066.0 51 205607.0 52 206977.0 53 197217.0 54 185548.0 55 161859.5 56 142615.0 57 119286.5 58 95188.0 59 89306.5 60 75092.0 61 54062.5 62 41003.5 63 33419.0 64 24989.5 65 13999.0 66 8547.0 67 6120.0 68 4381.0 69 3218.0 70 2411.0 71 1757.0 72 1297.0 73 1021.0 74 787.0 75 613.5 76 463.0 77 286.0 78 179.0 79 115.5 80 74.5 81 46.0 82 41.0 83 39.5 84 28.0 85 18.5 86 14.5 87 11.5 88 12.0 89 13.5 90 15.5 91 11.5 92 6.5 93 6.5 94 5.0 95 3.0 96 3.5 97 4.0 98 2.5 99 2.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9885748961661571 2 0.4432934079560902 3 0.47717118765784594 4 0.462493772629991 5 0.4294806744782146 6 0.48218190638336744 7 0.4983004573278546 8 0.5014487850226513 9 0.5148624347082288 10-11 0.5282760843938061 12-13 0.5268792911207626 14-15 0.5235646785125083 16-17 0.5287971104559732 18-19 0.5270677473560145 20-21 0.5257263823874567 22-23 0.4897534127761355 24-25 0.5234205649208451 26-27 0.5043975708212989 28-29 0.5101066861833422 30-31 0.5121575334493189 32-33 0.5144855222377249 34-35 0.5268571197989682 36-37 0.544505491947265 38-39 0.5340406280603353 40-41 0.5396278011525096 42-43 0.537255469720515 44-45 0.5465230822305502 46-47 0.5441507507985556 48-49 0.5168356823479252 50-51 0.5309255573482301 52-53 0.5247619298894027 54-55 0.5345062258180165 56-57 0.5214916599247372 58-59 0.5284977976117495 60-61 0.52257805469266 62-63 0.5172569374620178 64-65 0.5275111737919013 66-67 0.5252718702906727 68-69 0.5454034304799359 70-71 0.5292848795354488 72-73 0.53312051820587 74-75 0.5309477286700244 76-77 0.5192966990670973 78-79 0.5486626147837044 80-81 0.5237087921041714 82-83 0.5239194196612178 84-85 0.5324886355347312 86-87 0.8363909433697883 88-89 1.1020477211096038 90-91 1.091593942883571 92-93 1.0914276579701134 94-95 1.0964605480174294 96-97 1.0891550974861934 98-99 1.0848316897362966 100 1.081627933737014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4510331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.14937747858049 #Duplication Level Percentage of deduplicated Percentage of total 1 74.66824509372042 24.005375971811834 2 12.41681784767376 7.983859281352783 3 4.885790436112448 4.712253630354524 4 2.4974142271596698 3.211612508373344 5 1.3858147601844406 2.227654092027904 6 0.8667630455047537 1.6719535400649819 7 0.5819034827503173 1.3095484306128422 8 0.41629218534211104 1.0706827686357379 9 0.3026323921751434 0.8756498711956048 >10 1.5451270789123128 9.138705052235734 >50 0.17192012079325852 3.8966511422050556 >100 0.1971324702803152 14.073873635773907 >500 0.03693095234727166 8.305346265228998 >1k 0.026044589525537155 14.448242969298155 >5k 8.957133963809075E-4 1.909415803828772 >10k+ 2.756041219633561E-4 1.159175036999961 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 15012 0.33283588277667425 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 13775 0.3054099577170722 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 12875 0.28545576810216367 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 10668 0.2365236609020491 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 9903 0.21956259972937686 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8621 0.19113896518902937 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8565 0.18989737116854616 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6977 0.1546893121591298 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 6636 0.14712889142725888 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 6510 0.1443353048811717 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6438 0.14273896971197902 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5754 0.12757378560464852 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 5750 0.12748510031747115 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5333 0.1182396591292302 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5327 0.11810663119846414 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 5255 0.11651029602927146 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5130 0.11373888080497861 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4970 0.11019146931788378 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4768 0.10571286231542652 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 4735 0.10498120869621319 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4728 0.1048260094436528 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.434264358868562E-5 0.0 4 0.0 0.0 0.0 6.651396538302843E-5 0.0 5 0.0 0.0 0.0 6.651396538302843E-5 0.0 6 0.0 0.0 0.0 6.651396538302843E-5 0.0 7 0.0 0.0 0.0 6.651396538302843E-5 0.0 8 0.0 0.0 0.0 6.651396538302843E-5 0.0 9 0.0 0.0 0.0 6.651396538302843E-5 0.0 10-11 0.0 0.0 0.0 6.651396538302843E-5 1.1085660897171404E-5 12-13 0.0 0.0 0.0 6.651396538302843E-5 9.977094807454265E-5 14-15 0.0 0.0 0.0 1.1085660897171405E-4 1.3302793076605686E-4 16-17 0.0 0.0 0.0 1.3302793076605686E-4 1.5519925256039967E-4 18-19 0.0 0.0 0.0 1.3302793076605686E-4 1.5519925256039967E-4 20-21 0.0 0.0 0.0 1.3302793076605686E-4 1.7737057435474247E-4 22-23 0.0 0.0 0.0 1.6628491345757108E-4 1.995418961490853E-4 24-25 0.0 0.0 0.0 2.438845397377709E-4 1.995418961490853E-4 26-27 0.0 0.0 0.0 4.6559775768119897E-4 2.5497020063494234E-4 28-29 0.0 0.0 0.0 0.0013857076121464257 2.6605586153211373E-4 30-31 0.0 0.0 0.0 0.005775629327426302 2.771415224292851E-4 32-33 0.0 0.0 0.0 0.016473292093196708 3.1039850512079933E-4 34-35 0.0 0.0 0.0 0.032392301141534845 3.5474114870948494E-4 36-37 0.0 0.0 0.0 0.0633102093837459 3.5474114870948494E-4 38-39 0.0 0.0 0.0 0.11431533517163153 3.5474114870948494E-4 40-41 0.0 0.0 0.0 0.17202728580230586 3.5474114870948494E-4 42-43 0.0 0.0 0.0 0.23165705576819084 3.5474114870948494E-4 44-45 0.0 0.0 0.0 0.30419053501838333 3.5474114870948494E-4 46-47 0.0 0.0 0.0 0.3990616209763762 3.5474114870948494E-4 48-49 0.0 0.0 0.0 0.5128226731031492 3.990837922981706E-4 50-51 0.0 0.0 0.0 0.6358402520790603 4.2125511409251337E-4 52-53 0.0 0.0 0.0 0.7564744139620796 4.877690794755418E-4 54-55 2.217132179434281E-5 0.0 0.0 0.8952114600901796 4.877690794755418E-4 56-57 2.217132179434281E-5 0.0 0.0 1.0679703995116987 4.877690794755418E-4 58-59 2.217132179434281E-5 0.0 0.0 1.2740195786074238 4.877690794755418E-4 60-61 2.217132179434281E-5 0.0 0.0 1.4868753534940118 4.877690794755418E-4 62-63 2.217132179434281E-5 0.0 0.0 1.7145859139828097 4.877690794755418E-4 64-65 2.217132179434281E-5 0.0 0.0 1.9589693084609532 4.877690794755418E-4 66-67 2.217132179434281E-5 0.0 0.0 2.2354567768973053 4.877690794755418E-4 68-69 2.217132179434281E-5 0.0 0.0 2.5530166189576775 4.877690794755418E-4 70-71 2.217132179434281E-5 0.0 0.0 2.910518097230558 4.877690794755418E-4 72-73 2.217132179434281E-5 0.0 0.0 3.275214169425703 4.877690794755418E-4 74-75 2.217132179434281E-5 0.0 0.0 3.6385245340087016 4.988547403727132E-4 76-77 2.217132179434281E-5 0.0 0.0 4.05916993675187 5.099404012698846E-4 78-79 2.217132179434281E-5 0.0 0.0 4.516076536289686 5.099404012698846E-4 80-81 2.217132179434281E-5 0.0 0.0 5.01214877577721 5.099404012698846E-4 82-83 2.217132179434281E-5 2.217132179434281E-5 0.0 5.529361370595639 5.321117230642275E-4 84-85 2.217132179434281E-5 2.217132179434281E-5 0.0 6.041563246688547 5.321117230642275E-4 86-87 2.217132179434281E-5 2.217132179434281E-5 0.0 6.592298880060023 5.542830448585702E-4 88 2.217132179434281E-5 2.217132179434281E-5 0.0 7.030415284377133 5.76454366652913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4485 0.0 35.780388 94 GTACTAG 4505 0.0 33.39705 1 CAACGCA 8175 0.0 32.46536 5 GTATCAA 9035 0.0 32.31281 1 GCATCGT 4450 0.0 30.806684 8 GGTATCA 5910 0.0 30.405327 1 ATCAACG 8920 0.0 29.75916 3 TAGGCAT 5745 0.0 29.108475 5 CTAGGCA 6455 0.0 27.07323 4 CTGTGCG 6445 0.0 26.80902 9 CTAACGC 2360 0.0 26.676561 3 TATCAAC 10920 0.0 26.54662 2 AACGCAG 10755 0.0 26.319973 6 GTACATG 18210 0.0 26.18505 1 TAGTACT 6685 0.0 26.00122 4 TCTAACG 2405 0.0 25.787281 2 ACGCAGA 10125 0.0 25.78084 7 TATTCTC 2315 0.0 25.566957 5 CGCAGAG 10155 0.0 25.566267 8 CAGTACG 760 0.0 25.329481 9 >>END_MODULE