FastQCFastQC Report
Mon 16 Jan 2017
ERR1146449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510331
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC174250.38633528226642344No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT135860.3012195778979414No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA114820.25457111684264416No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC106280.23563680803027537No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC105640.23421784343543744No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA94470.20945247699115654No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC75360.1670830810421674No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG70070.15535445181296006No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT66110.1465746083824003No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG63140.13998972580948052No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC62730.13908070161591243No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.13870378914540862No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG62240.13799430684798963No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC61110.1354889474852289No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT59160.13116553973533207No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA58540.1297909177840828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58160.12894840755589776No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT55500.12305083595860261No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT54790.12147667211120426No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT54690.12125495889326082No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT54220.12021290676892671No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA53990.11970296636765684No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA51480.11413796459727678No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG51370.11389408005753902No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT51250.11362802419600691No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC47860.10611194610772468No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA46290.10263104858601287No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT45830.1016111677834731No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86000.039.803061
GTATCAA138850.035.5571441
GGGCACC47900.032.9933994
CAACGCA149250.031.6781655
ATCAACG154100.030.59953
TACCCCG3450.029.9695345
GTACTAG46750.029.4692761
TGAACCG4400.028.8394815
TATCAAC174850.028.1246362
CTGTGCG61550.027.569439
AACGCAG180500.027.5473656
TATTCTC23450.027.4570665
GTGGTAT23600.027.2955631
ACTGTGC72100.025.877778
TAGTACT64700.025.8679354
ACGCAGA183350.025.7093817
CAGTACG8250.025.639369
CTAACGC22400.025.605183
CGCAGAG184700.025.4705758
TAGGCAT60250.025.3514445
Produced by FastQC (version 0.11.5)