##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4510331
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80673325305837	31.0	31.0	34.0	30.0	34.0
2	31.75825632309469	31.0	31.0	34.0	30.0	34.0
3	31.95273650647813	31.0	31.0	34.0	30.0	34.0
4	35.701436546453024	37.0	35.0	37.0	33.0	37.0
5	35.616098020300505	37.0	35.0	37.0	33.0	37.0
6	35.651614482396084	37.0	35.0	37.0	33.0	37.0
7	35.568921660073286	37.0	35.0	37.0	33.0	37.0
8	35.548657958806125	37.0	35.0	37.0	33.0	37.0
9	37.128959936643234	39.0	37.0	39.0	33.0	39.0
10-11	37.076626859536475	39.0	37.0	39.0	33.0	39.0
12-13	37.01733287423916	39.0	37.0	39.0	33.0	39.0
14-15	38.21865169097346	40.0	37.0	41.0	33.0	41.0
16-17	38.21424092821569	40.0	37.0	41.0	33.0	41.0
18-19	38.25353726810737	40.0	37.5	41.0	33.0	41.0
20-21	38.26052666644643	40.0	38.0	41.0	33.0	41.0
22-23	38.233129564105155	40.0	37.0	41.0	33.0	41.0
24-25	38.16918503320488	40.0	37.0	41.0	33.0	41.0
26-27	38.03132530628018	40.0	37.0	41.0	33.0	41.0
28-29	37.96456900834994	40.0	37.0	41.0	33.0	41.0
30-31	37.84656137210329	40.0	37.0	41.0	33.0	41.0
32-33	37.74351682836581	40.0	36.5	41.0	32.0	41.0
34-35	37.660401753219446	40.0	36.0	41.0	32.0	41.0
36-37	37.58558496039426	40.0	36.0	41.0	32.0	41.0
38-39	37.47699093481165	40.0	36.0	41.0	31.5	41.0
40-41	37.356839996887146	40.0	36.0	41.0	31.0	41.0
42-43	37.245226791559205	39.0	36.0	41.0	31.0	41.0
44-45	37.065345536724465	39.0	35.0	41.0	31.0	41.0
46-47	36.85478094623211	39.0	35.0	41.0	30.5	41.0
48-49	36.623691698015065	39.0	35.0	41.0	30.0	41.0
50-51	36.427243588109164	39.0	35.0	41.0	30.0	41.0
52-53	36.23469064687271	38.0	35.0	40.5	29.5	41.0
54-55	36.03385305867795	38.0	35.0	40.0	29.0	41.0
56-57	35.78041755693762	38.0	34.0	40.0	29.0	41.0
58-59	35.50643578043385	37.0	34.0	40.0	28.0	41.0
60-61	35.24867986407206	37.0	34.0	40.0	28.0	41.0
62-63	34.97193354545376	36.0	34.0	39.5	28.0	41.0
64-65	34.69531781592083	36.0	33.5	39.0	28.0	41.0
66-67	34.46474981547917	35.0	33.0	39.0	28.0	41.0
68-69	34.268944341335484	35.0	33.0	38.5	27.5	41.0
70-71	34.05340206738707	35.0	33.0	38.0	27.0	40.0
72-73	33.84432694629285	35.0	33.0	37.0	27.0	40.0
74-75	33.66114050166163	35.0	33.0	37.0	27.0	39.0
76-77	33.50120656333205	35.0	33.0	36.0	27.0	39.0
78-79	33.37766862343362	35.0	33.0	36.0	27.0	39.0
80-81	33.222991727214705	35.0	33.0	36.0	27.0	37.5
82-83	33.03346960566752	35.0	33.0	35.0	27.0	37.0
84-85	32.892644131883	35.0	33.0	35.0	27.0	37.0
86-87	32.75977827347926	35.0	33.0	35.0	27.0	36.0
88-89	32.65358606718664	35.0	33.0	35.0	27.0	36.0
90-91	32.559525232183624	35.0	33.0	35.0	27.0	36.0
92-93	32.48201030035268	35.0	33.0	35.0	27.0	36.0
94-95	32.44195814010102	35.0	33.0	35.0	27.0	35.0
96-97	32.39072453884205	35.0	33.0	35.0	27.0	35.0
98-99	32.34845192071269	35.0	33.0	35.0	26.0	35.0
100	31.840090228411174	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5075283358938805
1101	2	0.6007653347917525
1101	3	0.48858985253385256
1101	4	0.3407951453793032
1101	5	0.3555177759517605
1101	6	0.3516846052461986
1101	7	0.40232083505232197
1101	8	0.42325076345180435
1101	9	0.4335805892891642
1101	10-11	0.49756184418683347
1101	12-13	0.5199470747110837
1101	14-15	0.6599619219162278
1101	16-17	0.6370739525403835
1101	18-19	0.6377142431471157
1101	20-21	0.6236028501491688
1101	22-23	0.6496980070639822
1101	24-25	0.6772715044251569
1101	26-27	0.6935630271423534
1101	28-29	0.7017108431874419
1101	30-31	0.7405832005072597
1101	32-33	0.7744700361292871
1101	34-35	0.7092505783365226
1101	36-37	0.7151135296077982
1101	38-39	0.7814190605814773
1101	40-41	0.8506209210904743
1101	42-43	0.8208645457345085
1101	44-45	0.7948010959776042
1101	46-47	0.889969184106981
1101	48-49	0.9018276650754231
1101	50-51	0.8866123947982274
1101	52-53	0.8590143769982745
1101	54-55	0.8279494078049012
1101	56-57	0.8500667782626081
1101	58-59	0.8639321850674904
1101	60-61	0.8533265063644393
1101	62-63	0.8202327586976068
1101	64-65	0.7884827051784526
1101	66-67	0.7805508306175355
1101	68-69	0.7695930417055337
1101	70-71	0.7113270162034127
1101	72-73	0.7222596289460697
1101	74-75	0.6975887829228213
1101	76-77	0.7294361969053469
1101	78-79	0.6614372744715098
1101	80-81	0.6501281957126608
1101	82-83	0.6322812770183148
1101	84-85	0.6095642260857801
1101	86-87	0.6341370190229085
1101	88-89	0.6704742020817633
1101	90-91	0.6317885747431262
1101	92-93	0.6769300123059949
1101	94-95	0.6388856237922909
1101	96-97	0.6163442874427787
1101	98-99	0.6695045335606906
1101	100	0.6904908407799581
1102	1	0.43837993555242605
1102	2	0.5344877239903241
1102	3	0.42457687961470825
1102	4	0.3323274759282171
1102	5	0.30809502048590076
1102	6	0.353360061690843
1102	7	0.3544931462530201
1102	8	0.34599790702724675
1102	9	0.4929429982180622
1102	10-11	0.49428433021335394
1102	12-13	0.5352471031371024
1102	14-15	0.6097339782090145
1102	16-17	0.6868894909515575
1102	18-19	0.6492264388374238
1102	20-21	0.6741191428630984
1102	22-23	0.6386288101049402
1102	24-25	0.6401695194087509
1102	26-27	0.675289754638392
1102	28-29	0.6557128987189245
1102	30-31	0.7633949575626033
1102	32-33	0.7861754896994881
1102	34-35	0.7465634970378048
1102	36-37	0.7424374293803027
1102	38-39	0.77153935302794
1102	40-41	0.7249419675502367
1102	42-43	0.7597446877835736
1102	44-45	0.7712600536630703
1102	46-47	0.7144650129788488
1102	48-49	0.7918849723147048
1102	50-51	0.7819242647471398
1102	52-53	0.7675413987991817
1102	54-55	0.8039515807167632
1102	56-57	0.8227366186860863
1102	58-59	0.8334010946110837
1102	60-61	0.794735337855144
1102	62-63	0.6902884362168109
1102	64-65	0.6765845111572375
1102	66-67	0.6628999852617596
1102	68-69	0.6822349981396201
1102	70-71	0.7137778199332985
1102	72-73	0.7590001012348893
1102	74-75	0.6810272773451587
1102	76-77	0.6561052886128564
1102	78-79	0.5779589163146142
1102	80-81	0.612548547847986
1102	82-83	0.6059001652423888
1102	84-85	0.5865757290726847
1102	86-87	0.5769136176900105
1102	88-89	0.6098780147293752
1102	90-91	0.6275563863822242
1102	92-93	0.5877278594255202
1102	94-95	0.6201432459178164
1102	96-97	0.6346252671042762
1102	98-99	0.5800568009702616
1102	100	0.6667328944472501
1103	1	0.3263209157024036
1103	2	-0.11159306724134055
1103	3	0.16445911037208205
1103	4	0.21058347002860955
1103	5	0.19779045375970128
1103	6	0.24627638020226073
1103	7	0.3393499328933629
1103	8	0.24115389597955783
1103	9	0.3311598551949331
1103	10-11	0.41659709785655963
1103	12-13	0.39832901924573605
1103	14-15	0.4893531495327821
1103	16-17	0.49198214424876596
1103	18-19	0.5132273271374785
1103	20-21	0.5010083773527612
1103	22-23	0.43713133735638365
1103	24-25	0.4543856552880712
1103	26-27	0.5133805863814658
1103	28-29	0.5558032870819787
1103	30-31	0.581592805152809
1103	32-33	0.5622972444488781
1103	34-35	0.5345263528510102
1103	36-37	0.5751262446609644
1103	38-39	0.6030685124876669
1103	40-41	0.5636789673708549
1103	42-43	0.4838397004697015
1103	44-45	0.5117598795671086
1103	46-47	0.5407279713575903
1103	48-49	0.5518841689359277
1103	50-51	0.535642412483476
1103	52-53	0.5350947943377165
1103	54-55	0.5321808315805256
1103	56-57	0.44637162643533657
1103	58-59	0.45036701345713936
1103	60-61	0.4891658823677574
1103	62-63	0.44776943819699255
1103	64-65	0.4422611958949787
1103	66-67	0.5396887222359155
1103	68-69	0.41461945166384595
1103	70-71	0.3912852234116144
1103	72-73	0.4074328104334839
1103	74-75	0.4398180140761312
1103	76-77	0.417841869767166
1103	78-79	0.3825171357192261
1103	80-81	0.43881373376031974
1103	82-83	0.43326837445498256
1103	84-85	0.4119825812080151
1103	86-87	0.42579940722104936
1103	88-89	0.4161764328761919
1103	90-91	0.3691788158817957
1103	92-93	0.47555028528770293
1103	94-95	0.4182346419693985
1103	96-97	0.4314495448810547
1103	98-99	0.458319661007522
1103	100	0.42262905669470996
1104	1	0.32535080094964997
1104	2	0.4339811523387951
1104	3	0.3536208448306901
1104	4	0.23876984321434236
1104	5	0.267271758865391
1104	6	0.278081884111522
1104	7	0.2993141399343884
1104	8	0.33821749279761093
1104	9	0.38893561520037423
1104	10-11	0.35990927574367504
1104	12-13	0.3742653750914968
1104	14-15	0.4756505419369077
1104	16-17	0.4941763669646946
1104	18-19	0.4863547734180216
1104	20-21	0.46778215030983716
1104	22-23	0.4760070980380533
1104	24-25	0.45131231342289624
1104	26-27	0.5421017642862083
1104	28-29	0.548916989238748
1104	30-31	0.5793264049089544
1104	32-33	0.5714355033351239
1104	34-35	0.511306030083226
1104	36-37	0.49989106121179816
1104	38-39	0.5462077987281333
1104	40-41	0.522162541990042
1104	42-43	0.5035419299672981
1104	44-45	0.5362145184342921
1104	46-47	0.5590815721062867
1104	48-49	0.5228292080717978
1104	50-51	0.5431307686490996
1104	52-53	0.5387235881476684
1104	54-55	0.5391668827767049
1104	56-57	0.48963430825702403
1104	58-59	0.46256957931513654
1104	60-61	0.4889913648098201
1104	62-63	0.41685602377477693
1104	64-65	0.3952547978970955
1104	66-67	0.40197858105396733
1104	68-69	0.38570308126099917
1104	70-71	0.4031793834597437
1104	72-73	0.37218041153993653
1104	74-75	0.4104969026194354
1104	76-77	0.4392165892189368
1104	78-79	0.37437830417963625
1104	80-81	0.2977290321702881
1104	82-83	0.3566140753071849
1104	84-85	0.3229568431431389
1104	86-87	0.32277645752013484
1104	88-89	0.32056352769308916
1104	90-91	0.284389417399872
1104	92-93	0.24526313863748328
1104	94-95	0.2829971238594311
1104	96-97	0.2570090791565036
1104	98-99	0.2751416958155417
1104	100	0.32470877091271433
1105	1	0.1267986382279247
1105	2	-0.366153235372213
1105	3	0.02246168786971836
1105	4	0.13554804039292634
1105	5	0.18555365409392266
1105	6	0.19448675089749656
1105	7	0.2711895388210266
1105	8	0.18936653232924527
1105	9	0.2947879858279592
1105	10-11	0.3247519143333477
1105	12-13	0.3086263091367414
1105	14-15	0.36540214754153766
1105	16-17	0.38489932031626495
1105	18-19	0.4199705944667258
1105	20-21	0.3612600625911
1105	22-23	0.2964132482078554
1105	24-25	0.34401768812089983
1105	26-27	0.3990947734044781
1105	28-29	0.40052271326376143
1105	30-31	0.4374373096482458
1105	32-33	0.3594013703616241
1105	34-35	0.39011267487968837
1105	36-37	0.40598804612812955
1105	38-39	0.41411166882213024
1105	40-41	0.3520675849011994
1105	42-43	0.3776593684153795
1105	44-45	0.39164090708077026
1105	46-47	0.33421796058199504
1105	48-49	0.3311918763060788
1105	50-51	0.34951622548482675
1105	52-53	0.30113985270766364
1105	54-55	0.2656939148806927
1105	56-57	0.3391929207494897
1105	58-59	0.2975766445833372
1105	60-61	0.309855266788702
1105	62-63	0.2870111340534578
1105	64-65	0.32462639279817296
1105	66-67	0.34596134813833146
1105	68-69	0.333951475077491
1105	70-71	0.27535653239681324
1105	72-73	0.26945284825281846
1105	74-75	0.230365596013705
1105	76-77	0.21142583722375008
1105	78-79	0.12956674292902903
1105	80-81	0.1468225120941895
1105	82-83	0.1362117530018665
1105	84-85	0.1733697604563389
1105	86-87	0.18544762239790913
1105	88-89	0.14290349111925593
1105	90-91	0.16957459414301468
1105	92-93	0.17437584616344992
1105	94-95	0.18864292367180724
1105	96-97	0.11928540958182055
1105	98-99	0.1614340407523045
1105	100	0.18267933268113268
1106	1	0.2612681058661934
1106	2	0.3258451501345121
1106	3	0.2647952041402384
1106	4	0.1497288665430716
1106	5	0.23965783537015284
1106	6	0.20515351338840304
1106	7	0.2242779105493895
1106	8	0.20577048086872196
1106	9	0.3130102356359501
1106	10-11	0.2607789795998201
1106	12-13	0.279781052944827
1106	14-15	0.32680004173124644
1106	16-17	0.37008284188615903
1106	18-19	0.350779503507745
1106	20-21	0.38921649450289664
1106	22-23	0.30763622686082925
1106	24-25	0.2739774455694217
1106	26-27	0.30431414558147907
1106	28-29	0.28386844204341344
1106	30-31	0.3069350018811008
1106	32-33	0.31100239742325897
1106	34-35	0.30997615074431906
1106	36-37	0.3284444569876328
1106	38-39	0.34518776052883027
1106	40-41	0.25884816626396656
1106	42-43	0.3204786664011863
1106	44-45	0.3024722585945767
1106	46-47	0.34204493176648754
1106	48-49	0.31469228501099167
1106	50-51	0.29384872979328946
1106	52-53	0.3054866979849109
1106	54-55	0.32391997225037983
1106	56-57	0.293388080609013
1106	58-59	0.361719177334173
1106	60-61	0.25299802603319677
1106	62-63	0.2897882584841369
1106	64-65	0.27738369903357807
1106	66-67	0.27731621942169227
1106	68-69	0.29247166568061544
1106	70-71	0.27558349791895864
1106	72-73	0.27471684766626225
1106	74-75	0.23073317634999313
1106	76-77	0.24160339518652307
1106	78-79	0.24458960644772532
1106	80-81	0.22979340112536306
1106	82-83	0.25692899018573456
1106	84-85	0.2525449024867399
1106	86-87	0.24823039465127295
1106	88-89	0.2031389900804399
1106	90-91	0.2350510257353946
1106	92-93	0.2256704352879879
1106	94-95	0.2123251044282739
1106	96-97	0.22359684707609517
1106	98-99	0.2781942449450483
1106	100	0.34565172078092843
1107	1	0.11660188969274188
1107	2	-0.4829673907030667
1107	3	-0.0019018056645023762
1107	4	0.03466266079425395
1107	5	0.1518360445293112
1107	6	0.16696993181841435
1107	7	0.20860242203417556
1107	8	0.21750396759313873
1107	9	0.2467264882025475
1107	10-11	0.3057568179397663
1107	12-13	0.23710454616601595
1107	14-15	0.3229830425000628
1107	16-17	0.3593715279910441
1107	18-19	0.3480846419227177
1107	20-21	0.2750297743727401
1107	22-23	0.2915038228093678
1107	24-25	0.26796764268812723
1107	26-27	0.3741978364239955
1107	28-29	0.2742420349501913
1107	30-31	0.3406538606068068
1107	32-33	0.35824954879828397
1107	34-35	0.32745780059195084
1107	36-37	0.3331436410736828
1107	38-39	0.4007394378714437
1107	40-41	0.3909766163997688
1107	42-43	0.3513067362239113
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2216	80-81	-0.7600520823225807
2216	82-83	-0.7002453856338775
2216	84-85	-0.8225564648435508
2216	86-87	-0.8004970454744083
2216	88-89	-0.7872775170305992
2216	90-91	-0.8562493299600717
2216	92-93	-0.8238686688276644
2216	94-95	-0.7926703896622733
2216	96-97	-0.8068322860859674
2216	98-99	-0.7656457028499304
2216	100	-0.7638475567478302
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	6.0
13	39.0
14	205.0
15	636.0
16	1611.0
17	3149.0
18	5655.0
19	8842.0
20	13076.0
21	18833.0
22	26540.0
23	31317.0
24	34471.0
25	38327.0
26	44658.0
27	56893.0
28	71658.0
29	89633.0
30	111587.0
31	137152.0
32	170101.0
33	214339.0
34	290464.0
35	396060.0
36	589809.0
37	988980.0
38	862055.0
39	303660.0
40	573.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15995614820912	21.580642414544887	13.750716228178323	24.50868520906767
2	15.073519548415234	20.247991147100958	39.943119547173595	24.735369757310217
3	18.174084267337378	26.39823459544623	29.62533487678375	25.802346260432646
4	12.463720804492755	17.187628558698833	38.749451944910845	31.599198691897566
5	12.891262269384352	38.64004115107722	35.05519698639309	13.413499593145332
6	29.970594175904164	38.74611419871402	17.671031239170695	13.612260386211123
7	26.330688654783536	32.13478437758781	23.739716627330907	17.79481034029774
8	26.155020551706738	35.16571178478919	19.593728265176104	19.08553939832797
9	25.91317577357405	14.740935864795732	21.423194883036302	37.92269347859392
10-11	25.68701720561085	25.87714737565824	27.37020852793287	21.065626890798036
12-13	26.062477454537152	23.5512981198054	27.77697468323278	22.60924974242467
14-15	23.319643164995448	24.645074671377756	25.372159530280697	26.663122633346102
16-17	22.071403366618306	27.228731982341774	26.683655590386536	24.016209060653384
18-19	21.540281633432226	27.258431809106693	28.971676358120945	22.22961019934014
20-21	23.04538605618039	26.48986947290751	28.25057290733623	22.214171563575864
22-23	23.428940932557936	26.03925886419587	28.01111501945494	22.520685183791255
24-25	22.946154062750605	26.138791144153277	28.683238547237444	22.231816245858674
26-27	22.82821841936615	26.48043981767605	27.664257342073135	23.027084420884666
28-29	22.080796287456508	26.94776724812436	28.30223546786256	22.669200996556572
30-31	22.906556082025908	26.527199445007472	28.150350827910415	22.415893645056208
32-33	23.23301770978671	26.701698833189845	27.43508181550312	22.630201641520323
34-35	23.13064186364955	26.587121532514995	28.07871474065594	22.203521863179517
36-37	22.97553106412811	26.163900166085373	27.749060989093703	23.111507780692815
38-39	23.158067556460935	26.128958162937487	28.376176826046688	22.336797454554887
40-41	22.610033746021198	25.496742518445796	28.94873400594793	22.944489729585076
42-43	22.858030667398772	25.66025344908187	28.070716889994657	23.4109989935247
44-45	23.00503000777548	26.456362071874544	27.857888922121237	22.680718998228734
46-47	22.991749386020672	26.32295722863799	27.151809922597696	23.533483462743643
48-49	23.816645598239674	26.081936614686796	27.270470823083194	22.83094696399034
50-51	23.303303383199967	26.093309570107998	27.075266435574953	23.528120611117078
52-53	22.728642310287206	26.527376815581828	26.969317772908465	23.774663101222504
54-55	22.805074238766633	26.534128902834215	26.81247993486925	23.848316923529904
56-57	23.764681728624687	25.989232927867402	26.615828502843087	23.630256840664828
58-59	22.56301605274483	26.413830152590094	27.53542487792992	23.487728916735158
60-61	22.74641819088223	26.996643590357284	26.795216886231383	23.461721332529102
62-63	23.282348900779123	26.288259110029838	26.33344426384671	24.095947725344327
64-65	22.401781598734104	26.41850453991071	26.838096804868645	24.34161705648654
66-67	23.015284244105366	26.128658850093263	26.82154591314917	24.0345109926522
68-69	22.54688732088178	26.05133479718273	27.126817344786197	24.27496053714929
70-71	22.47835032209151	25.713787804832357	27.262669455698656	24.545192417377475
72-73	23.065058861533664	25.764639003212846	27.539076400379486	23.631225734874004
74-75	23.112965230603525	26.23805599473385	27.02787852827196	23.621100246390665
76-77	22.82708710719504	26.16150317392394	26.589068309760787	24.42234140912024
78-79	22.61768038117114	26.23366243118544	26.76482326048542	24.383833927158
80-81	22.29441956609608	26.642682347379885	27.29481977282891	23.768078313695117
82-83	23.135714920776962	25.594729860333405	28.11387462443803	23.155680594451606
84-85	23.309982479098863	26.11235106014534	26.814317915442913	23.763348545312883
86-87	21.96979952046154	26.729773635539907	27.34985653425201	23.950570309746546
88-89	22.846472134754876	25.482151967976492	27.2465335990281	24.424842298240527
90-91	22.51572327044025	25.56170850115354	27.332016270383715	24.5905519580225
92-93	22.80061855126053	25.879383391135885	27.051473283290754	24.268524774312827
94-95	21.718183999067016	25.722740718831854	27.748909043421428	24.810166238679702
96-97	22.030637260200532	25.59097127966109	27.36382691589859	25.014564544239796
98-99	21.438919344378608	26.272070683835064	27.273340021839264	25.015669949947068
100	22.216728917544806	25.47635679694379	26.554023347629734	25.752890937881673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	122.0
1	86.5
2	76.5
3	94.5
4	69.5
5	57.5
6	116.5
7	288.5
8	507.0
9	613.0
10	531.5
11	429.5
12	404.0
13	450.0
14	582.0
15	757.0
16	1011.0
17	1383.0
18	1834.5
19	2442.5
20	3169.5
21	3879.0
22	4687.5
23	5731.0
24	6761.0
25	7745.5
26	9449.0
27	11541.5
28	14811.0
29	21611.0
30	31179.5
31	40986.5
32	54800.0
33	69611.5
34	81733.5
35	95165.5
36	108553.0
37	130890.0
38	142089.5
39	135900.0
40	128532.0
41	119656.5
42	118696.0
43	127392.0
44	140713.5
45	165823.5
46	190465.5
47	207343.5
48	219100.0
49	214830.5
50	203244.0
51	204905.5
52	205754.5
53	196777.5
54	188637.5
55	165317.5
56	145117.5
57	121697.0
58	96083.0
59	91948.5
60	77288.5
61	53817.5
62	39800.5
63	32207.5
64	23703.0
65	12560.5
66	7434.0
67	5181.0
68	3798.0
69	2907.5
70	2073.0
71	1469.5
72	1084.0
73	900.0
74	743.0
75	515.0
76	334.0
77	189.0
78	107.5
79	48.0
80	22.5
81	9.0
82	2.5
83	3.5
84	3.0
85	1.5
86	1.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013191936467633973
2	0.0037691247050382778
3	0.0019954189614908528
4	0.026694271440388745
5	0.0026827299371154803
6	0.0
7	3.5474114870948494E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008946128344017323
16-17	0.0030485567467221364
18-19	0.0
20-21	6.651396538302843E-5
22-23	8.314245672878555E-4
24-25	0.0
26-27	1.6628491345757105E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.3234077498968476E-4
36-37	0.0
38-39	0.0
40-41	4.766834185783704E-4
42-43	8.092532454935127E-4
44-45	0.0
46-47	0.0
48-49	6.097113493444273E-4
50-51	1.1085660897171404E-5
52-53	0.0
54-55	4.2125511409251337E-4
56-57	1.2194226986888545E-4
58-59	2.6605586153211373E-4
60-61	1.2194226986888545E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001696106117267225
70-71	9.86623819848255E-4
72-73	0.0
74-75	3.1039850512079933E-4
76-77	0.0031261563730023358
78-79	4.65597757681199E-4
80-81	0.003591754130683535
82-83	0.0019954189614908528
84-85	0.006407511998565073
86-87	0.008192303403009669
88-89	0.008735500786971067
90-91	0.006562711251125472
92-93	0.00971103894592215
94-95	0.0015298212038096538
96-97	0.005199174960773389
98-99	0.002350160110200338
100	0.08502701908130468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4510331.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.65316228229753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.21666300333035	16.324501388133104
2	16.028492658553198	7.90306061303853
3	6.685970434477463	4.944909424074487
4	3.363112755508736	3.3164545814088675
5	1.8752480303775183	2.311539700622788
6	1.2046465488733598	1.7819008117310762
7	0.8388690373272353	1.4476542157576124
8	0.5669141778380993	1.1180981781102355
9	0.4250979819713651	0.9432008581875527
>10	2.177283061512112	9.893622478854471
>50	0.2418265055103757	4.185046039089224
>100	0.2809474183714944	15.503461758978798
>500	0.05352631813068312	9.349117188952595
>1k	0.03915683675315355	16.8641329427118
>5k	0.001796185172163007	2.7032615541351634
>10k+	4.4904629304075175E-4	1.4100382662137196
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	17425	0.38633528226642344	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13586	0.3012195778979414	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11482	0.25457111684264416	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10628	0.23563680803027537	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10564	0.23421784343543744	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9447	0.20945247699115654	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7536	0.1670830810421674	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7007	0.15535445181296006	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6611	0.1465746083824003	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6314	0.13998972580948052	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6273	0.13908070161591243	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6256	0.13870378914540862	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6224	0.13799430684798963	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6111	0.1354889474852289	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5916	0.13116553973533207	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5854	0.1297909177840828	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5816	0.12894840755589776	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5550	0.12305083595860261	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5479	0.12147667211120426	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5469	0.12125495889326082	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5422	0.12021290676892671	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5399	0.11970296636765684	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5148	0.11413796459727678	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5137	0.11389408005753902	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5125	0.11362802419600691	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4786	0.10611194610772468	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4629	0.10263104858601287	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4583	0.1016111677834731	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.217132179434281E-5	0.0
5	0.0	0.0	0.0	2.217132179434281E-5	0.0
6	0.0	0.0	0.0	2.217132179434281E-5	0.0
7	0.0	0.0	0.0	2.217132179434281E-5	0.0
8	0.0	0.0	0.0	2.217132179434281E-5	0.0
9	0.0	0.0	0.0	2.217132179434281E-5	0.0
10-11	0.0	0.0	0.0	8.868528717737123E-5	0.0
12-13	0.0	0.0	0.0	1.1085660897171405E-4	1.3302793076605686E-4
14-15	0.0	0.0	0.0	1.1085660897171405E-4	1.6628491345757108E-4
16-17	0.0	0.0	0.0	1.1085660897171405E-4	1.7737057435474247E-4
18-19	0.0	0.0	0.0	1.1085660897171405E-4	1.995418961490853E-4
20-21	0.0	0.0	0.0	1.1085660897171405E-4	2.6605586153211373E-4
22-23	0.0	0.0	0.0	1.4411359166322825E-4	2.6605586153211373E-4
24-25	0.0	0.0	0.0	2.438845397377709E-4	2.6605586153211373E-4
26-27	0.0	0.0	0.0	4.988547403727132E-4	2.6605586153211373E-4
28-29	0.0	0.0	0.0	0.0014743928993237968	2.6605586153211373E-4
30-31	0.0	0.0	0.0	0.005952999901781044	2.6605586153211373E-4
32-33	0.0	0.0	0.0	0.016683919650242966	2.6605586153211373E-4
34-35	0.0	0.0	0.0	0.03282464191652453	2.6605586153211373E-4
36-37	0.0	0.0	0.0	0.06414163395103376	2.6605586153211373E-4
38-39	0.0	0.0	0.0	0.11616664054145916	2.771415224292851E-4
40-41	0.0	0.0	0.0	0.175075842549028	2.882271833264565E-4
42-43	0.0	0.0	0.0	0.23614674843154526	2.882271833264565E-4
44-45	0.0	0.0	0.0	0.31030981983362194	3.1039850512079933E-4
46-47	0.0	0.0	0.0	0.406810497943499	3.3256982691514216E-4
48-49	0.0	0.0	0.0	0.5227332539452204	3.3256982691514216E-4
50-51	0.0	0.0	0.0	0.6481231643531262	3.3256982691514216E-4
52-53	0.0	0.0	0.0	0.7708857731284023	3.3256982691514216E-4
54-55	0.0	0.0	0.0	0.9113632680173585	3.5474114870948494E-4
56-57	0.0	0.0	0.0	1.0858626561997333	3.5474114870948494E-4
58-59	0.0	0.0	0.0	1.2947941071287228	3.5474114870948494E-4
60-61	0.0	0.0	0.0	1.5107206100838275	3.658268096066563E-4
62-63	0.0	0.0	0.0	1.7412247571187125	3.7691247050382776E-4
64-65	0.0	0.0	0.0	1.98927750535382	3.879981314009992E-4
66-67	0.0	0.0	0.0	2.2696338694432847	3.990837922981706E-4
68-69	0.0	0.0	0.0	2.5909295792260036	3.990837922981706E-4
70-71	0.0	0.0	0.0	2.953663489442349	4.10169453195342E-4
72-73	0.0	0.0	0.0	3.324312561539275	4.434264358868562E-4
74-75	0.0	0.0	0.0	3.6939528384945586	4.65597757681199E-4
76-77	0.0	0.0	0.0	4.123045514841372	4.877690794755418E-4
78-79	0.0	0.0	0.0	4.590017894473821	4.877690794755418E-4
80-81	0.0	0.0	0.0	5.098805387010399	4.877690794755418E-4
82-83	0.0	0.0	0.0	5.6290214620612105	4.877690794755418E-4
84-85	0.0	0.0	0.0	6.152974138705119	4.877690794755418E-4
86-87	0.0	0.0	0.0	6.715704457167334	5.321117230642275E-4
88	0.0	0.0	0.0	7.1635762430739565	5.76454366652913E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8600	0.0	39.80306	1
GTATCAA	13885	0.0	35.557144	1
GGGCACC	4790	0.0	32.99339	94
CAACGCA	14925	0.0	31.678165	5
ATCAACG	15410	0.0	30.5995	3
TACCCCG	345	0.0	29.969534	5
GTACTAG	4675	0.0	29.469276	1
TGAACCG	440	0.0	28.839481	5
TATCAAC	17485	0.0	28.124636	2
CTGTGCG	6155	0.0	27.56943	9
AACGCAG	18050	0.0	27.547365	6
TATTCTC	2345	0.0	27.457066	5
GTGGTAT	2360	0.0	27.295563	1
ACTGTGC	7210	0.0	25.87777	8
TAGTACT	6470	0.0	25.867935	4
ACGCAGA	18335	0.0	25.709381	7
CAGTACG	825	0.0	25.63936	9
CTAACGC	2240	0.0	25.60518	3
CGCAGAG	18470	0.0	25.470575	8
TAGGCAT	6025	0.0	25.351444	5
>>END_MODULE