##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146448_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4369912 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.213318254463704 31.0 30.0 34.0 26.0 34.0 2 30.18499113940967 31.0 30.0 34.0 26.0 34.0 3 30.44686597807919 31.0 30.0 34.0 26.0 34.0 4 33.896325372227174 35.0 35.0 37.0 28.0 37.0 5 34.12667806582833 37.0 35.0 37.0 30.0 37.0 6 34.29209764407155 37.0 35.0 37.0 30.0 37.0 7 34.130214750319915 37.0 35.0 37.0 29.0 37.0 8 34.08785851980544 37.0 35.0 37.0 29.0 37.0 9 35.42504883393533 39.0 35.0 39.0 28.0 39.0 10-11 35.36293488289924 38.5 35.0 39.0 28.5 39.0 12-13 35.3587674076732 39.0 35.0 39.0 28.0 39.0 14-15 36.32015759127415 39.0 36.0 41.0 27.0 41.0 16-17 36.14632056663841 39.0 35.5 41.0 27.0 41.0 18-19 36.083345728701175 39.0 35.0 41.0 27.0 41.0 20-21 36.422333447446995 39.0 35.5 41.0 27.5 41.0 22-23 36.12622622606588 39.0 35.0 41.0 27.0 41.0 24-25 36.21988772313951 39.0 35.0 41.0 27.0 41.0 26-27 36.28604305990601 39.0 35.0 41.0 27.5 41.0 28-29 36.12448683177144 39.0 35.0 41.0 27.5 41.0 30-31 36.05873859702437 39.0 35.0 41.0 27.0 41.0 32-33 36.170666136983996 39.0 35.0 41.0 28.0 41.0 34-35 36.21535673944922 39.0 35.0 41.0 28.0 41.0 36-37 36.134301445887246 39.0 35.0 41.0 28.0 41.0 38-39 35.969145602932045 39.0 35.0 41.0 27.0 41.0 40-41 35.92368416114558 39.0 34.0 41.0 27.0 41.0 42-43 35.760021140013805 39.0 34.0 41.0 27.0 41.0 44-45 35.567146203401805 38.0 34.0 40.0 26.5 41.0 46-47 35.43581209415659 38.0 33.5 40.0 26.5 41.0 48-49 35.304697096875174 38.0 33.0 40.0 26.0 41.0 50-51 35.086921429996764 38.0 33.0 40.0 26.0 41.0 52-53 34.898027237161756 37.5 33.0 40.0 26.0 41.0 54-55 34.700959882029665 37.0 33.0 40.0 26.0 41.0 56-57 34.545401829602056 37.0 33.0 40.0 26.0 41.0 58-59 34.27872849613448 36.0 32.5 40.0 25.0 41.0 60-61 34.02706816521706 36.0 32.0 39.5 25.0 41.0 62-63 33.75612277775845 35.0 32.0 39.0 25.0 41.0 64-65 33.46563145894014 35.0 32.0 39.0 24.0 41.0 66-67 33.179680963827195 35.0 31.0 38.0 24.0 40.0 68-69 32.8937347022091 35.0 31.0 37.5 23.0 40.0 70-71 32.844328901817704 35.0 31.0 37.0 24.0 40.0 72-73 32.63979961152536 35.0 31.0 37.0 24.0 40.0 74-75 32.38108490514226 35.0 31.0 36.0 24.0 39.0 76-77 32.15717135722642 35.0 31.0 36.0 23.0 39.0 78-79 31.944343387235257 35.0 31.0 35.5 23.0 39.0 80-81 31.740523264541714 34.5 30.5 35.0 23.0 37.5 82-83 31.513327613919913 34.0 30.0 35.0 23.0 37.0 84-85 31.33771744144962 34.0 30.0 35.0 22.5 36.5 86-87 31.186966121972247 34.0 30.0 35.0 22.0 36.0 88-89 31.20794835227803 34.0 31.0 35.0 22.5 36.0 90-91 31.138489516493696 34.0 31.0 35.0 22.0 36.0 92-93 31.004502836670397 34.0 30.0 35.0 20.5 35.5 94-95 30.987738654691444 34.0 30.0 35.0 21.0 35.0 96-97 30.909073226188536 34.0 30.0 35.0 20.0 35.0 98-99 30.83848439053235 34.0 30.0 35.0 20.0 35.0 100 30.32596011086722 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9124525667213845 1101 2 1.105571626789672 1101 3 1.0499369435705077 1101 4 1.1851871243718577 1101 5 1.086143773554447 1101 6 0.8376605983484069 1101 7 0.8553258628274918 1101 8 0.8950853469948186 1101 9 1.0271950306184081 1101 10-11 1.140463845495752 1101 12-13 1.0208794057276762 1101 14-15 1.3517021455625766 1101 16-17 1.437989398225362 1101 18-19 1.5766481923081486 1101 20-21 1.1667949019856891 1101 22-23 1.5309946274398243 1101 24-25 1.3840707778660786 1101 26-27 1.2833204588653189 1101 28-29 1.442560655663371 1101 30-31 1.4426851459354069 1101 32-33 1.3187813250890486 1101 34-35 1.1994921185263863 1101 36-37 1.1125137163005618 1101 38-39 1.3359125393044735 1101 40-41 1.2156283359725037 1101 42-43 1.2751057208240013 1101 44-45 1.2497977947703234 1101 46-47 1.2545736094614313 1101 48-49 1.2536360047595778 1101 50-51 1.2104136471428504 1101 52-53 1.1805048791759205 1101 54-55 1.217715121580767 1101 56-57 1.012289353496918 1101 58-59 1.0174801547531374 1101 60-61 1.0546116360108044 1101 62-63 1.0309135126259008 1101 64-65 1.0392574752094035 1101 66-67 1.050802646026547 1101 68-69 1.0350881202691724 1101 70-71 0.9820306431950883 1101 72-73 0.916346279088657 1101 74-75 0.9347427861185196 1101 76-77 0.9920501182811137 1101 78-79 0.9659881450085948 1101 80-81 0.9334131000557058 1101 82-83 0.9672379608924935 1101 84-85 0.9564456878511436 1101 86-87 0.9386971638142363 1101 88-89 0.9291424419454728 1101 90-91 0.9431537682409221 1101 92-93 1.0375949077047046 1101 94-95 1.0340537007526756 1101 96-97 0.9793034885050851 1101 98-99 0.988091217856681 1101 100 1.1122550363962809 1102 1 0.9036384942938867 1102 2 1.0126184486236802 1102 3 0.9604184541617151 1102 4 1.09525741096693 1102 5 1.012058881555994 1102 6 0.7706843684937184 1102 7 0.8271550903500113 1102 8 0.88931028981564 1102 9 1.0331205297207404 1102 10-11 1.012854317025095 1102 12-13 1.0051458687512635 1102 14-15 1.2475095413260888 1102 16-17 1.4473391990023217 1102 18-19 1.463344770558038 1102 20-21 1.1544125552845586 1102 22-23 1.43449806783444 1102 24-25 1.306398605442915 1102 26-27 1.1949199570707876 1102 28-29 1.344777182737296 1102 30-31 1.459505107456991 1102 32-33 1.280974012100728 1102 34-35 1.2303039624525312 1102 36-37 1.137694837285757 1102 38-39 1.235558373943725 1102 40-41 1.1703405354302063 1102 42-43 1.24231287742343 1102 44-45 1.2262756736999378 1102 46-47 1.1359477421299928 1102 48-49 1.1698662934119852 1102 50-51 1.2886967688770454 1102 52-53 1.1528514115378172 1102 54-55 1.2426258770531717 1102 56-57 1.0577258681304542 1102 58-59 1.0304838981210693 1102 60-61 1.1246858484007163 1102 62-63 1.013062115814435 1102 64-65 0.9819242350986244 1102 66-67 1.021050204158037 1102 68-69 1.113492752787451 1102 70-71 1.0209248428231632 1102 72-73 0.9500635326556264 1102 74-75 0.9846846224743473 1102 76-77 0.9706781634844077 1102 78-79 0.983925089590997 1102 80-81 1.0031534591554312 1102 82-83 0.9766495617970783 1102 84-85 0.9662634899846552 1102 86-87 0.9307318586791204 1102 88-89 0.902816712679865 1102 90-91 0.8833248356552978 1102 92-93 1.0384416658902076 1102 94-95 0.9787843156840346 1102 96-97 1.0443793845265752 1102 98-99 1.0097837859292405 1102 100 1.0601667707055569 1103 1 0.7083813940130241 1103 2 0.8921403475134255 1103 3 0.8772528077985768 1103 4 0.806467409598632 1103 5 0.8173176992257112 1103 6 0.6135004311600198 1103 7 0.6675090298561557 1103 8 0.6375230833340666 1103 9 0.7491918141374754 1103 10-11 0.8676376151223479 1103 12-13 0.8021529017859095 1103 14-15 1.0207983160548508 1103 16-17 1.231345387991773 1103 18-19 1.2869727529627468 1103 20-21 0.9859419095432358 1103 22-23 1.20855534290871 1103 24-25 1.0845842955418163 1103 26-27 0.9704892979706088 1103 28-29 1.1909773741747216 1103 30-31 1.240295497497506 1103 32-33 1.019932951078573 1103 34-35 0.9851962414961193 1103 36-37 0.95084727362471 1103 38-39 1.0760983509226136 1103 40-41 1.0037270501562716 1103 42-43 0.9240030732525142 1103 44-45 0.9612996961051579 1103 46-47 0.9709173480874327 1103 48-49 0.919852579235986 1103 50-51 1.0228601293363937 1103 52-53 1.0562112366072611 1103 54-55 1.0619621795056133 1103 56-57 0.9278294008086831 1103 58-59 0.9456751999672761 1103 60-61 0.9165005853113968 1103 62-63 0.8409698561888703 1103 64-65 0.9151037826492612 1103 66-67 0.9404121544572419 1103 68-69 0.9528881804811675 1103 70-71 0.8436078912289062 1103 72-73 0.8211395056062827 1103 74-75 0.782097236679931 1103 76-77 0.756436435859797 1103 78-79 0.8019433896004386 1103 80-81 0.8680010338813311 1103 82-83 0.706706130435439 1103 84-85 0.7012483178982407 1103 86-87 0.7499724735853697 1103 88-89 0.6923103447827543 1103 90-91 0.7560444058467901 1103 92-93 0.8693960961768425 1103 94-95 0.7405071894335116 1103 96-97 0.7733248127034109 1103 98-99 0.78681816705679 1103 100 0.9342913621244122 1104 1 0.5703958723778193 1104 2 0.7778828433170304 1104 3 0.7470669636581491 1104 4 0.9102890156003909 1104 5 0.7709652305481853 1104 6 0.4549053691642513 1104 7 0.5175136556781368 1104 8 0.5591970844741354 1104 9 0.7400781483123495 1104 10-11 0.7896117072535063 1104 12-13 0.5928218422408875 1104 14-15 0.8492007813650702 1104 16-17 0.9972695660899475 1104 18-19 0.9281968876383289 1104 20-21 0.8369218211296996 1104 22-23 1.0367953369252092 1104 24-25 0.9383824737366027 1104 26-27 0.8029270113672595 1104 28-29 1.0248861806047245 1104 30-31 1.1190375395603525 1104 32-33 0.8922558935107645 1104 34-35 0.8191761278725451 1104 36-37 0.7682467344804778 1104 38-39 0.9205908608147624 1104 40-41 0.7668052425578864 1104 42-43 0.8183432854731976 1104 44-45 0.7971255901224197 1104 46-47 0.7461629955633171 1104 48-49 0.7498251855284295 1104 50-51 0.76898289200075 1104 52-53 0.8465502960165594 1104 54-55 0.8083976680471778 1104 56-57 0.7854404371636647 1104 58-59 0.7019682793034931 1104 60-61 0.6813556369818059 1104 62-63 0.6818858768700196 1104 64-65 0.6303981599636401 1104 66-67 0.7384359825979416 1104 68-69 0.7348563042465841 1104 70-71 0.5962807720160015 1104 72-73 0.5815393112381315 1104 74-75 0.6497164171101275 1104 76-77 0.7005643023410855 1104 78-79 0.5694150276165502 1104 80-81 0.6569780844277666 1104 82-83 0.6462733207685183 1104 84-85 0.6121743521000127 1104 86-87 0.5703024314957652 1104 88-89 0.5440607554215333 1104 90-91 0.5030932588679562 1104 92-93 0.5099320527129905 1104 94-95 0.48544937183618586 1104 96-97 0.5523810482417382 1104 98-99 0.5904231997599396 1104 100 0.6525813922812667 1105 1 0.5863671980219856 1105 2 0.7084001935243194 1105 3 0.6622749191534396 1105 4 0.7987841475279893 1105 5 0.6040998512947766 1105 6 0.3334704352854203 1105 7 0.42849858527719675 1105 8 0.4836747051547192 1105 9 0.60487194553329 1105 10-11 0.6734185681275235 1105 12-13 0.5659205455675433 1105 14-15 0.7853177844570141 1105 16-17 0.9501886190641144 1105 18-19 0.9202905357355533 1105 20-21 0.7322496795542932 1105 22-23 0.9751440639971918 1105 24-25 0.8237682381101266 1105 26-27 0.6442259434448161 1105 28-29 0.8985012108707053 1105 30-31 0.9199748346580776 1105 32-33 0.7270901286903921 1105 34-35 0.6604604403787917 1105 36-37 0.6251308233164607 1105 38-39 0.727971550880973 1105 40-41 0.6662570749472039 1105 42-43 0.6276160724929767 1105 44-45 0.7190729843547174 1105 46-47 0.6494618156220966 1105 48-49 0.7304425109548873 1105 50-51 0.7569783161667374 1105 52-53 0.7357641130111503 1105 54-55 0.7037557246093087 1105 56-57 0.6512892206021021 1105 58-59 0.7218076598988787 1105 60-61 0.6739754570192673 1105 62-63 0.6312638003379476 1105 64-65 0.5755126981326057 1105 66-67 0.7373192636337436 1105 68-69 0.7623287844083961 1105 70-71 0.6171083866620535 1105 72-73 0.565224573598222 1105 74-75 0.5644442018145384 1105 76-77 0.5876620256565701 1105 78-79 0.5790678724800458 1105 80-81 0.6758092145043015 1105 82-83 0.5638301506537324 1105 84-85 0.5423255090814578 1105 86-87 0.6422967297609325 1105 88-89 0.566773754056797 1105 90-91 0.6313477381648376 1105 92-93 0.6362814988271204 1105 94-95 0.5353922759318976 1105 96-97 0.5645749235493476 1105 98-99 0.6142584162770142 1105 100 0.661581824747163 1106 1 0.5287380337777279 1106 2 0.5999414939958179 1106 3 0.632623021202761 1106 4 0.7754953527149127 1106 5 0.572149287232655 1106 6 0.34675292313459494 1106 7 0.3510734890495115 1106 8 0.4070149161909242 1106 9 0.6518150069631972 1106 10-11 0.6340334727469994 1106 12-13 0.507647728959796 1106 14-15 0.6537139095172577 1106 16-17 0.871419810406131 1106 18-19 0.8084357901951549 1106 20-21 0.649818934104573 1106 22-23 0.9478770793672737 1106 24-25 0.7495896522958887 1106 26-27 0.6337579842234149 1106 28-29 0.8509982286601598 1106 30-31 0.8051330561280707 1106 32-33 0.6783941926600789 1106 34-35 0.6565565162301965 1106 36-37 0.4931933743187997 1106 38-39 0.6492760831748043 1106 40-41 0.6362333713822608 1106 42-43 0.6478543172339997 1106 44-45 0.6358314813305483 1106 46-47 0.6109763897996245 1106 48-49 0.6093441108833773 1106 50-51 0.5730771224174731 1106 52-53 0.530700027675536 1106 54-55 0.6172007438031386 1106 56-57 0.6184418605631592 1106 58-59 0.5924830220819786 1106 60-61 0.5709251058109714 1106 62-63 0.5561099071375182 1106 64-65 0.5468402439341631 1106 66-67 0.5172574806432166 1106 68-69 0.5134281380315997 1106 70-71 0.509934406631757 1106 72-73 0.4458258023574615 1106 74-75 0.46426525426714704 1106 76-77 0.4790795135559591 1106 78-79 0.5101615326340294 1106 80-81 0.5334013204883412 1106 82-83 0.5167496843142594 1106 84-85 0.547242783252031 1106 86-87 0.5078098971762728 1106 88-89 0.5807362664497404 1106 90-91 0.5014175801089209 1106 92-93 0.5492004637373142 1106 94-95 0.5928461119490471 1106 96-97 0.5801892579270138 1106 98-99 0.5404272852105869 1106 100 0.6153943461436597 1107 1 0.5066017117250183 1107 2 0.7041088075011075 1107 3 0.575585272542952 1107 4 0.7552177139322254 1107 5 0.5844685244179715 1107 6 0.4545044446795572 1107 7 0.40919329343107336 1107 8 0.5181377004828178 1107 9 0.5871922781489971 1107 10-11 0.5985593984001554 1107 12-13 0.43588152496025145 1107 14-15 0.643662127527989 1107 16-17 0.7400725785264584 1107 18-19 0.8972117139830047 1107 20-21 0.6019800730989431 1107 22-23 0.8635055549966779 1107 24-25 0.6090603505663168 1107 26-27 0.5349482996712638 1107 28-29 0.7249219493577712 1107 30-31 0.7477912933141013 1107 32-33 0.6443155447850728 1107 34-35 0.5128835787789541 1107 36-37 0.5300012900832058 1107 38-39 0.5538137433831949 1107 40-41 0.38964732547850645 1107 42-43 0.588389509707909 1107 44-45 0.5125379920531472 1107 46-47 0.42766779879586636 1107 48-49 0.4169281523696142 1107 50-51 0.5488792805358713 1107 52-53 0.5625208477201014 1107 54-55 0.4751429473578952 1107 56-57 0.3899814965476551 1107 58-59 0.4434427380554382 1107 60-61 0.4350975988389507 1107 62-63 0.4094458976237618 1107 64-65 0.4756884764581528 1107 66-67 0.47532142825301804 1107 68-69 0.49526265300372074 1107 70-71 0.35978838958121173 1107 72-73 0.34256276925111706 1107 74-75 0.38453041959428447 1107 76-77 0.2918434086725483 1107 78-79 0.2892965583619542 1107 80-81 0.4531492555989338 1107 82-83 0.3674637520374837 1107 84-85 0.34081136607733953 1107 86-87 0.4200009520058039 1107 88-89 0.5048546645138714 1107 90-91 0.5344754263757778 1107 92-93 0.6039449137415609 1107 94-95 0.587419797759491 1107 96-97 0.5553958431413513 1107 98-99 0.5874575377628624 1107 100 0.5570513072223839 1108 1 0.3888690990723873 1108 2 0.4790266658448452 1108 3 0.48806956224594344 1108 4 0.5257599470909753 1108 5 0.46474956683645985 1108 6 0.2870102323846382 1108 7 0.21535619474077805 1108 8 0.25478189823845554 1108 9 0.3620766498759309 1108 10-11 0.40400252010294224 1108 12-13 0.26414062110247727 1108 14-15 0.4581906554465718 1108 16-17 0.6031521750374864 1108 18-19 0.6909303390525494 1108 20-21 0.32789321051973985 1108 22-23 0.5244333055711508 1108 24-25 0.39399052467886264 1108 26-27 0.3848686705260107 1108 28-29 0.540618084571939 1108 30-31 0.5300408738992957 1108 32-33 0.46875511682529947 1108 34-35 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10-11 -1.2855736015216976 2216 12-13 -1.530193423161272 2216 14-15 -1.9240206813148575 2216 16-17 -1.759474531349241 2216 18-19 -3.7748620359410268 2216 20-21 -1.8598506387122384 2216 22-23 -1.3868705872291116 2216 24-25 -1.5154276866844825 2216 26-27 -1.4590568367012153 2216 28-29 -1.492591207867818 2216 30-31 -1.692490314106287 2216 32-33 -1.5508996346158526 2216 34-35 -1.8040849319459085 2216 36-37 -1.8123462777371273 2216 38-39 -1.9322198445981158 2216 40-41 -1.886706042312312 2216 42-43 -1.8106283962745238 2216 44-45 -1.6935820559243737 2216 46-47 -1.9082994603955896 2216 48-49 -1.9649703208588534 2216 50-51 -1.7093549162190698 2216 52-53 -1.7545881349159629 2216 54-55 -1.6207958019273434 2216 56-57 -1.7337114634839068 2216 58-59 -1.7706020343428506 2216 60-61 -1.7327080376507453 2216 62-63 -1.5994256943044078 2216 64-65 -1.6328507272986883 2216 66-67 -1.6823069717564039 2216 68-69 -1.7263466713542996 2216 70-71 -1.8408780612610656 2216 72-73 -1.7490168544454079 2216 74-75 -1.7864524248471838 2216 76-77 -1.711258911935829 2216 78-79 -1.8875295144651325 2216 80-81 -1.8415040671664435 2216 82-83 -1.9439953448986778 2216 84-85 -1.8567487329538075 2216 86-87 -1.8376583332185348 2216 88-89 -1.7146193627569062 2216 90-91 -1.856587521165249 2216 92-93 -1.7865880856841478 2216 94-95 -1.7293620372688103 2216 96-97 -1.7105213609156493 2216 98-99 -1.628222197442934 2216 100 -1.5729797888554415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 17867.0 1 2857.0 2 452.0 3 457.0 4 407.0 5 216.0 6 228.0 7 269.0 8 499.0 9 970.0 10 1948.0 11 4263.0 12 7846.0 13 11315.0 14 14080.0 15 17010.0 16 19739.0 17 22505.0 18 24969.0 19 28104.0 20 32169.0 21 35809.0 22 40314.0 23 45996.0 24 51851.0 25 57960.0 26 65642.0 27 74547.0 28 87444.0 29 102853.0 30 120625.0 31 143921.0 32 174598.0 33 218887.0 34 296682.0 35 391879.0 36 570112.0 37 828328.0 38 650830.0 39 203322.0 40 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.609778899478016 21.013551695088594 12.341992227449376 24.034677177984012 2 15.473254358386404 19.671868887306466 40.86381165904852 23.991065095258605 3 18.58644949261648 26.70812680483698 28.78953396318345 25.91588973936309 4 12.121255314081408 16.52645982632621 40.06440349407462 31.287881365517762 5 13.043069575095794 38.7105166450671 35.06545022728375 13.180963552553349 6 30.042468044342996 38.3933517241189 18.137826038068788 13.426354193469317 7 27.042083078825165 31.60685571410699 23.771461477889066 17.57959972917878 8 26.130931326529815 35.30583867776168 19.855906269850433 18.70732372585808 9 26.18417808168763 14.878361941424565 21.540047954128912 37.39741202275889 10-11 25.783329234652435 25.730222752763044 27.551244821916725 20.9352031906678 12-13 25.996066229964516 23.50288418958011 27.530708136117642 22.97034144433773 14-15 23.348698768798332 24.72491683813177 24.89190193587924 27.034482457190663 16-17 22.364826106525562 27.255279216117774 26.30065113792417 24.0792435394325 18-19 21.800239523382515 28.043203006418732 27.791487212000277 22.36507025819848 20-21 23.473679924472464 27.15678588385985 26.708755231165043 22.660778960502633 22-23 24.065787422198774 26.771290043513034 26.395546870221143 22.767375664067046 24-25 23.45077961925154 26.83817017327786 27.114226377819623 22.596823829650976 26-27 23.282124053373874 27.062683309538908 26.12881877503661 23.526373862050605 28-29 22.428931701037893 27.69743967013375 26.70578925439108 23.167839374437275 30-31 23.23283495096949 27.2818744323016 26.6410494620134 22.84424115471551 32-33 23.643465147807667 27.467099285700158 25.67912999594707 23.210305570545103 34-35 23.661495927844765 27.188730241656184 26.667066946310307 22.482706884188747 36-37 23.280075349074465 26.827749434185925 26.319117871901742 23.573057344837864 38-39 23.4738663096431 26.868435289152398 26.967717994326012 22.689980406878494 40-41 23.057350925498266 26.04028466958898 27.625247544399816 23.277116860512937 42-43 23.040732120770386 26.29479010218806 26.88289601554908 23.781581761492475 44-45 23.205230802222314 27.21253435837447 26.70996907113547 22.87226576826775 46-47 23.130389065352716 26.930906070496306 26.259604687789405 23.679100176361565 48-49 23.825834150205864 26.461609521243112 26.600589988414846 23.111966340136174 50-51 23.390363698416703 26.395802627705788 26.516997473611998 23.696836200265512 52-53 22.851843357355037 27.019770459951953 26.375079048023704 23.753307134669306 54-55 22.83612749869611 26.89386941614594 26.388384345802724 23.881618739355226 56-57 24.009530316204987 26.290043200419582 26.09204127733381 23.608385206041618 58-59 22.816179511113337 26.70410699075839 27.085193128233975 23.3945203698943 60-61 22.86090096796329 27.36973188758017 26.310260769710126 23.459106374746415 62-63 23.3420216171617 26.635994107317078 25.8426625137107 24.179321761810524 64-65 22.454288483049943 26.805602762848768 26.395534813102252 24.34457394099903 66-67 23.256619102670438 26.32121242584432 26.301313738916114 24.120854732569125 68-69 22.533100927846426 26.506167139826047 26.651652575808942 24.309079356518584 70-71 22.519572079382897 26.10909983503114 26.811269965752455 24.56005811983351 72-73 23.187429040751745 26.242366309037806 27.164931436255447 23.405273213955002 74-75 23.348339747235215 26.562169297562715 26.722942444202737 23.366548510999337 76-77 23.00316244156683 26.47449873184897 26.346706004728482 24.175632821855718 78-79 22.846472584127053 26.496979051474167 26.428087895359077 24.228460469039707 80-81 22.41760441030413 26.792666592670848 27.162902560734558 23.626826436290465 82-83 23.29494714806593 25.774122680898543 28.137323012157673 22.79360715887786 84-85 23.539952616644356 26.293379719758143 26.747117451182213 23.419550212415285 86-87 22.142031083469977 26.95388017228518 27.285225342292126 23.618863401952716 88-89 23.237328588435236 25.635060107570045 27.066477549466832 24.06113375452788 90-91 22.829744355847094 25.740145991481032 27.289239129215105 24.14087052345677 92-93 23.06322651635617 25.985961488140557 27.257416444618123 23.693395550885153 94-95 21.880325526119034 25.957141397848403 27.935658765592574 24.22687431043999 96-97 22.284366806771537 25.846543234797213 27.45630133555589 24.412788622875365 98-99 21.622972181238662 26.63888588460584 27.29499431178366 24.44314762237184 100 22.42331848938754 25.772552194783415 26.556937134925683 25.24719218090336 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14759.0 1 8676.5 2 2370.5 3 1642.0 4 841.5 5 454.5 6 341.0 7 311.5 8 293.5 9 289.0 10 294.5 11 276.5 12 262.5 13 277.0 14 285.0 15 328.0 16 400.0 17 461.0 18 572.0 19 737.5 20 883.5 21 1129.5 22 1584.5 23 2233.5 24 3020.5 25 4058.5 26 5959.5 27 8260.0 28 12221.0 29 20197.0 30 30988.5 31 41753.5 32 56406.0 33 70685.0 34 82432.0 35 95751.0 36 107870.0 37 128682.5 38 138132.5 39 129611.5 40 121521.0 41 113773.5 42 112864.5 43 120447.0 44 133041.5 45 157644.5 46 182796.5 47 199028.5 48 208293.0 49 204128.0 50 194818.0 51 201105.0 52 205309.0 53 196247.5 54 185526.0 55 161535.5 56 142198.0 57 119448.5 58 94884.0 59 88851.0 60 73382.5 61 51504.0 62 38795.0 63 31763.0 64 23314.0 65 12232.5 66 7210.0 67 5093.0 68 3756.5 69 2763.5 70 2021.0 71 1538.5 72 1212.0 73 964.0 74 722.0 75 559.0 76 392.5 77 247.0 78 156.5 79 112.0 80 77.0 81 45.0 82 33.5 83 31.5 84 28.5 85 25.5 86 23.5 87 17.5 88 14.5 89 11.0 90 4.5 91 3.0 92 4.5 93 6.5 94 7.5 95 5.5 96 3.5 97 3.0 98 3.5 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.987365420630896 2 0.44243453872755334 3 0.47678305650090896 4 0.4610619161209654 5 0.42891023892471974 6 0.480444457462759 7 0.4955706201864019 8 0.4981793683717201 9 0.5131453448032821 10-11 0.5259831319257687 12-13 0.5248847116372137 14-15 0.522150102793832 16-17 0.5259488062917513 18-19 0.5250906654413178 20-21 0.5237634075926472 22-23 0.4881105157266325 24-25 0.521520799503514 26-27 0.5012572335552753 28-29 0.5082596628948134 30-31 0.5106052478859986 32-33 0.513465717387444 34-35 0.5252050842213757 36-37 0.5432718095925043 38-39 0.5316697452946422 40-41 0.5370703117133709 42-43 0.5358460307667523 44-45 0.544862230635308 46-47 0.5429628788863483 48-49 0.515765534866606 50-51 0.5307315112981681 52-53 0.5233743837404505 54-55 0.5330770962893532 56-57 0.5201935416548434 58-59 0.5262005276078786 60-61 0.5204452629709705 62-63 0.5145755795540047 64-65 0.5251707585873583 66-67 0.5238435007386877 68-69 0.5440040897848745 70-71 0.5270357847023006 72-73 0.531521000880567 74-75 0.5287062988911447 76-77 0.5180424685897564 78-79 0.5475167463326494 80-81 0.5221958703058551 82-83 0.5231684299363466 84-85 0.5317727221966941 86-87 0.8356117926402179 88-89 1.1001823377679 90-91 1.0909487422172346 92-93 1.0906169277550668 94-95 1.09527377210342 96-97 1.0882255752518586 98-99 1.0842209179498352 100 1.0792437010173204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4369912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.19839918177148 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1903526264747 23.458186358589174 2 12.251573282781374 7.644589477618793 3 4.955939578114271 4.638521438362475 4 2.4190180258119907 3.018779599887331 5 1.3323735310653237 2.0783960640701182 6 0.8334548020149298 1.5601473367935657 7 0.5411976508979308 1.1819150244265453 8 0.38722340790267273 0.9664600361818807 9 0.2716886605716426 0.7628626157107431 >10 1.3815621678293768 7.922374673218807 >50 0.15817336136417207 3.5098933979160827 >100 0.20573385137915073 14.359578653990882 >500 0.041353687463277264 9.133137308341377 >1k 0.028742279229437145 15.712884062003491 >5k 0.0011731542542627407 2.2891346937869264 >10k+ 4.399328453485278E-4 1.7631392591018398 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 16344 0.3740121082529808 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 13743 0.3144914588669062 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 13262 0.3034843722253446 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 12236 0.28000563855748123 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 10986 0.2514009435430279 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10505 0.24039385690146622 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9766 0.22348276120892135 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8637 0.19764700067186708 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 7194 0.1646257407471821 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 6783 0.1552205170264298 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6753 0.15453400434608294 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6242 0.1428404050241744 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 5990 0.1370736985092606 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5823 0.13325211125532962 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 5789 0.1324740635509365 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5679 0.12995685038966462 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5382 0.12316037485423047 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5367 0.12281711851405704 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5320 0.12174158198151358 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5286 0.12096353427712046 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5042 0.11537989781029916 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5017 0.11480780391001008 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4838 0.11071161158394036 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 4802 0.1098877963675241 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 4794 0.10970472631943161 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 4767 0.10908686490711941 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4634 0.10604332535758157 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 4609 0.1054712314572925 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 4523 0.10350322844029812 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4506 0.10311420458810154 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 4462 0.10210731932359278 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4404 0.10078006147492215 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2883756011562703E-5 2 0.0 0.0 0.0 0.0 2.2883756011562703E-5 3 0.0 0.0 0.0 0.0 2.2883756011562703E-5 4 0.0 0.0 0.0 0.0 2.2883756011562703E-5 5 0.0 0.0 0.0 0.0 4.576751202312541E-5 6 0.0 0.0 0.0 0.0 4.576751202312541E-5 7 0.0 0.0 0.0 0.0 4.576751202312541E-5 8 0.0 0.0 0.0 0.0 6.865126803468812E-5 9 0.0 0.0 0.0 0.0 6.865126803468812E-5 10-11 0.0 0.0 0.0 0.0 6.865126803468812E-5 12-13 0.0 0.0 0.0 0.0 1.8307004809250163E-4 14-15 0.0 0.0 0.0 0.0 2.5172131612718974E-4 16-17 0.0 0.0 0.0 0.0 2.9748882815031514E-4 18-19 0.0 0.0 0.0 0.0 3.318144621676592E-4 20-21 0.0 0.0 0.0 0.0 3.6614009618500325E-4 22-23 0.0 0.0 0.0 5.7209390028906755E-5 3.89023852196566E-4 24-25 0.0 0.0 0.0 1.6018629208093893E-4 3.89023852196566E-4 26-27 0.0 0.0 0.0 4.920007542485981E-4 4.0046573020234734E-4 28-29 0.0 0.0 0.0 0.0014302347507226692 4.119076082081287E-4 30-31 0.0 0.0 0.0 0.006144288489104586 4.119076082081287E-4 32-33 0.0 0.0 0.0 0.01700263071659109 4.119076082081287E-4 34-35 0.0 0.0 0.0 0.03501214669769094 4.8055887624281677E-4 36-37 0.0 0.0 0.0 0.06741554521006374 4.8055887624281677E-4 38-39 0.0 0.0 0.0 0.11938455511232263 4.8055887624281677E-4 40-41 0.0 0.0 0.0 0.17657106138521783 4.8055887624281677E-4 42-43 0.0 0.0 0.0 0.23876911022464525 4.8055887624281677E-4 44-45 0.0 0.0 0.0 0.3147202964270219 4.920007542485981E-4 46-47 0.0 0.0 0.0 0.4116558868920015 5.034426322543795E-4 48-49 0.0 0.0 0.0 0.5240952220548148 5.720939002890676E-4 50-51 0.0 0.0 0.0 0.6476446207612419 5.949776563006303E-4 52-53 0.0 0.0 0.0 0.7674639672377841 6.407451683237557E-4 54-55 0.0 0.0 0.0 0.9036566411405996 6.407451683237557E-4 56-57 0.0 0.0 0.0 1.075433555641395 6.636289243353184E-4 58-59 0.0 0.0 0.0 1.2830464320562975 6.865126803468812E-4 60-61 0.0 0.0 0.0 1.49716973705649 6.865126803468812E-4 62-63 0.0 0.0 0.0 1.722929431988562 6.865126803468812E-4 64-65 0.0 0.0 0.0 1.9649709193228606 6.865126803468812E-4 66-67 0.0 0.0 0.0 2.238443245539041 6.865126803468812E-4 68-69 0.0 0.0 0.0 2.5484037207156574 6.865126803468812E-4 70-71 0.0 0.0 0.0 2.895904997629243 6.865126803468812E-4 72-73 0.0 0.0 0.0 3.262319698886385 7.322801923700065E-4 74-75 0.0 0.0 0.0 3.6131276785436413 7.894895823989134E-4 76-77 0.0 0.0 0.0 4.021556955838012 8.009314604046947E-4 78-79 0.0 0.0 0.0 4.467275313553225 8.238152164162574E-4 80-81 0.0 0.0 0.0 4.953097453678701 8.695827284393827E-4 82-83 0.0 0.0 0.0 5.462741583812214 9.611177524856335E-4 84-85 0.0 0.0 0.0 5.964433608731709 9.611177524856335E-4 86-87 0.0 1.1441878005781352E-5 0.0 6.503197318389935 0.001006885264508759 88 0.0 2.2883756011562703E-5 0.0 6.931192207074193 0.0010526527765318844 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4550 0.0 39.750374 94 GTACTAG 4765 0.0 35.150974 1 GCATCGT 4860 0.0 32.649223 8 TAGGCAT 5890 0.0 31.267502 5 GTATCAA 8965 0.0 30.103544 1 CAACGCA 8040 0.0 29.859823 5 GGTATCA 6000 0.0 29.645721 1 TAGTACT 6510 0.0 29.51853 4 CTAGGCA 6570 0.0 29.463493 4 CTGTGCG 6690 0.0 29.12389 9 ATCAACG 8705 0.0 27.529531 3 AGGGCTA 5295 0.0 27.50541 5 TATTCTC 2585 0.0 27.261711 5 ACTGTGC 7555 0.0 27.092209 8 CTATTGA 5295 0.0 26.954731 9 GTACTGT 7305 0.0 26.667685 6 TACCCCG 370 0.0 26.664902 5 CTAACGC 2205 0.0 26.637829 3 CTAGTAC 7600 0.0 26.276817 3 GCTATTG 5415 0.0 26.268578 8 >>END_MODULE