##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4369912
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78615610566071	31.0	31.0	34.0	30.0	34.0
2	31.739044859484583	31.0	31.0	34.0	30.0	34.0
3	31.93094460483415	31.0	31.0	34.0	30.0	34.0
4	35.68621816640701	37.0	35.0	37.0	33.0	37.0
5	35.599103368671955	37.0	35.0	37.0	33.0	37.0
6	35.63905566061742	37.0	35.0	37.0	33.0	37.0
7	35.55047630249762	37.0	35.0	37.0	33.0	37.0
8	35.531339761532955	37.0	35.0	37.0	33.0	37.0
9	37.10832895490802	39.0	37.0	39.0	33.0	39.0
10-11	37.052971661671904	39.0	37.0	39.0	33.0	39.0
12-13	36.99354575103572	39.0	37.0	39.0	33.0	39.0
14-15	38.18602754471944	40.0	37.0	41.0	33.0	41.0
16-17	38.18585328491741	40.0	37.0	41.0	33.0	41.0
18-19	38.22602835022765	40.0	37.0	41.0	33.0	41.0
20-21	38.23411066401337	40.0	37.0	41.0	33.0	41.0
22-23	38.20442116454519	40.0	37.0	41.0	33.0	41.0
24-25	38.13867281537935	40.0	37.0	41.0	33.0	41.0
26-27	37.99466362251688	40.0	37.0	41.0	33.0	41.0
28-29	37.92670607554568	40.0	37.0	41.0	33.0	41.0
30-31	37.805450201285524	40.0	37.0	41.0	32.5	41.0
32-33	37.696242967821775	40.0	36.0	41.0	32.0	41.0
34-35	37.609447398483084	40.0	36.0	41.0	32.0	41.0
36-37	37.53299849058745	40.0	36.0	41.0	32.0	41.0
38-39	37.41897251020158	40.0	36.0	41.0	31.0	41.0
40-41	37.29897787415399	39.5	36.0	41.0	31.0	41.0
42-43	37.18040031469741	39.0	35.5	41.0	31.0	41.0
44-45	36.994872322371705	39.0	35.0	41.0	31.0	41.0
46-47	36.777851590604115	39.0	35.0	41.0	30.0	41.0
48-49	36.53444451512982	39.0	35.0	41.0	30.0	41.0
50-51	36.33391965330195	39.0	35.0	41.0	30.0	41.0
52-53	36.142264992979264	38.0	35.0	40.0	29.0	41.0
54-55	35.937370935616094	38.0	35.0	40.0	29.0	41.0
56-57	35.67553980492056	37.5	34.0	40.0	28.5	41.0
58-59	35.38809088146398	37.0	34.0	40.0	28.0	41.0
60-61	35.125527813832406	36.5	34.0	40.0	28.0	41.0
62-63	34.845063127129336	36.0	34.0	39.0	28.0	41.0
64-65	34.56557305959479	35.5	33.0	39.0	27.0	41.0
66-67	34.34175665322322	35.0	33.0	39.0	27.0	41.0
68-69	34.148050921849226	35.0	33.0	38.5	27.0	41.0
70-71	33.94650201193983	35.0	33.0	37.5	27.0	40.0
72-73	33.74382779332856	35.0	33.0	37.0	26.5	40.0
74-75	33.561699068539596	35.0	33.0	36.5	26.5	39.0
76-77	33.40462508169501	35.0	33.0	36.0	27.0	39.0
78-79	33.29174363236605	35.0	33.0	36.0	27.0	39.0
80-81	33.14441366324996	35.0	33.0	36.0	27.0	38.0
82-83	32.956153464875264	35.0	33.0	35.0	27.0	37.0
84-85	32.81626735275218	35.0	33.0	35.0	27.0	37.0
86-87	32.690362185783144	35.0	33.0	35.0	27.0	36.0
88-89	32.58404677256659	35.0	33.0	35.0	27.0	36.0
90-91	32.49621125093594	35.0	33.0	35.0	26.5	36.0
92-93	32.418318835711105	35.0	33.0	35.0	26.0	36.0
94-95	32.38259637722682	35.0	33.0	35.0	26.0	35.0
96-97	32.33261505037172	35.0	33.0	35.0	26.0	35.0
98-99	32.291918121005644	35.0	33.0	35.0	26.0	35.0
100	31.781260354899594	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5284890412764867
1101	2	0.5957173161391616
1101	3	0.4861234109902668
1101	4	0.322157641428511
1101	5	0.3553673088054481
1101	6	0.39305540080166423
1101	7	0.41639047496886405
1101	8	0.43702182877783
1101	9	0.5029773668711854
1101	10-11	0.5368949851535874
1101	12-13	0.5407334928065808
1101	14-15	0.6808280841011438
1101	16-17	0.6914686094121834
1101	18-19	0.6611845168881061
1101	20-21	0.6383999750663918
1101	22-23	0.6922729318932568
1101	24-25	0.7164246575019959
1101	26-27	0.6967536705644619
1101	28-29	0.7285599395387123
1101	30-31	0.7927666551270676
1101	32-33	0.8159552148085254
1101	34-35	0.7851361744570795
1101	36-37	0.8026939294018121
1101	38-39	0.78082166402573
1101	40-41	0.7628208327883002
1101	42-43	0.821759241830307
1101	44-45	0.7485059992625835
1101	46-47	0.7887584377287098
1101	48-49	0.8144321287782788
1101	50-51	0.7669746823000665
1101	52-53	0.7733956870349203
1101	54-55	0.749169867145703
1101	56-57	0.7644750533904272
1101	58-59	0.701806121861857
1101	60-61	0.7148573916848591
1101	62-63	0.8111096274342131
1101	64-65	0.7650532703513448
1101	66-67	0.6921314060814083
1101	68-69	0.6477098162323998
1101	70-71	0.6353668380459752
1101	72-73	0.6513313456585195
1101	74-75	0.64483138470613
1101	76-77	0.6484008190302646
1101	78-79	0.6250159428654243
1101	80-81	0.6064770191944149
1101	82-83	0.5624189145469956
1101	84-85	0.5578488811346958
1101	86-87	0.5731828636124021
1101	88-89	0.6292122282214905
1101	90-91	0.6343247884685255
1101	92-93	0.6535255806232314
1101	94-95	0.5512499389655474
1101	96-97	0.6256275295163363
1101	98-99	0.600109309527987
1101	100	0.5995885364600433
1102	1	0.4831242517585608
1102	2	0.5995138450453901
1102	3	0.48915209942921933
1102	4	0.3873947481860185
1102	5	0.3814432257216467
1102	6	0.3591031018208497
1102	7	0.3650444135416251
1102	8	0.43456501252110513
1102	9	0.41906926824171364
1102	10-11	0.46788067709803016
1102	12-13	0.4948113368580991
1102	14-15	0.6649399262592723
1102	16-17	0.703528996735848
1102	18-19	0.7145077002134883
1102	20-21	0.7300687111131481
1102	22-23	0.679708961930217
1102	24-25	0.687803563808508
1102	26-27	0.7557822151513705
1102	28-29	0.7577515994949593
1102	30-31	0.7815486863180539
1102	32-33	0.7826892870109958
1102	34-35	0.7597792566199075
1102	36-37	0.7453103008904947
1102	38-39	0.7881009596167914
1102	40-41	0.6820909805139124
1102	42-43	0.7442609375165148
1102	44-45	0.7364812936771088
1102	46-47	0.6778929064485197
1102	48-49	0.7459530267043988
1102	50-51	0.7502008297813703
1102	52-53	0.6924079056193193
1102	54-55	0.710525560231325
1102	56-57	0.7363793210548977
1102	58-59	0.6869617151350482
1102	60-61	0.686522153718208
1102	62-63	0.6315613911889599
1102	64-65	0.6210630313962113
1102	66-67	0.6446042951931759
1102	68-69	0.6132179916901208
1102	70-71	0.5979515620830895
1102	72-73	0.5921071542436778
1102	74-75	0.6003389092132139
1102	76-77	0.5634765342321302
1102	78-79	0.591211031965301
1102	80-81	0.5315539077146951
1102	82-83	0.532385900902753
1102	84-85	0.5381594328032904
1102	86-87	0.5858748538658247
1102	88-89	0.5222839838755107
1102	90-91	0.5191166530322207
1102	92-93	0.5301039952611077
1102	94-95	0.49974156179350615
1102	96-97	0.5252926113994931
1102	98-99	0.5350539063966764
1102	100	0.5626572945266055
1103	1	0.2900304848203845
1103	2	-0.14555890865829824
1103	3	0.17652163943393617
1103	4	0.1308446317557852
1103	5	0.195907917796319
1103	6	0.2360468914653353
1103	7	0.33923733428397185
1103	8	0.3195460105951966
1103	9	0.40369513149455116
1103	10-11	0.3868477019852108
1103	12-13	0.4534490703536349
1103	14-15	0.5422058237165075
1103	16-17	0.5705026193884208
1103	18-19	0.49346787112951773
1103	20-21	0.4917803641731737
1103	22-23	0.499899186122704
1103	24-25	0.5307639370086363
1103	26-27	0.5395706526360726
1103	28-29	0.5568901349581097
1103	30-31	0.646748388097059
1103	32-33	0.585495663261284
1103	34-35	0.5455395744334766
1103	36-37	0.5607829459405025
1103	38-39	0.5404346487394847
1103	40-41	0.585604661495374
1103	42-43	0.6377854581515194
1103	44-45	0.5920340466576803
1103	46-47	0.6454596983817211
1103	48-49	0.5682980128795734
1103	50-51	0.6094378065349062
1103	52-53	0.5480695148718979
1103	54-55	0.4746708951205889
1103	56-57	0.50383121390729
1103	58-59	0.5543790615105593
1103	60-61	0.5239704411448969
1103	62-63	0.5876954109479726
1103	64-65	0.49584033187910137
1103	66-67	0.5590278369476991
1103	68-69	0.5391823631959198
1103	70-71	0.4993502989451457
1103	72-73	0.4391911113382534
1103	74-75	0.5096250115477758
1103	76-77	0.4336204886649071
1103	78-79	0.4274075112246294
1103	80-81	0.4374089530821266
1103	82-83	0.4065309856641548
1103	84-85	0.38695842163216554
1103	86-87	0.42863662475867415
1103	88-89	0.43534884818777186
1103	90-91	0.43430577307769624
1103	92-93	0.4739176979982318
1103	94-95	0.45886118104434104
1103	96-97	0.4446979444685226
1103	98-99	0.46783624933273416
1103	100	0.4401125605987595
1104	1	0.3529063179213665
1104	2	0.4119621820432222
1104	3	0.39540091926016885
1104	4	0.283189855794717
1104	5	0.2642022153386634
1104	6	0.30732547144830846
1104	7	0.2789897964538923
1104	8	0.2586650557554435
1104	9	0.32645567310163415
1104	10-11	0.34636363510430357
1104	12-13	0.38829995340771717
1104	14-15	0.46120315061668293
1104	16-17	0.4815581586837965
1104	18-19	0.46453130359456907
1104	20-21	0.48630444037182485
1104	22-23	0.46057894259777754
1104	24-25	0.4418483802239521
1104	26-27	0.5340321617548369
1104	28-29	0.5365618178902878
1104	30-31	0.5576387080856549
1104	32-33	0.5811665868656917
1104	34-35	0.5688976854190315
1104	36-37	0.5903294429387387
1104	38-39	0.539158107065326
1104	40-41	0.566295926967733
1104	42-43	0.5530037493565487
1104	44-45	0.5772925894835339
1104	46-47	0.5714926057982481
1104	48-49	0.5342926058253568
1104	50-51	0.49259009094421913
1104	52-53	0.501804922872779
1104	54-55	0.5134099737102176
1104	56-57	0.5590140840205393
1104	58-59	0.5575541118141345
1104	60-61	0.4761950692282113
1104	62-63	0.4497168788242405
1104	64-65	0.3961927187534684
1104	66-67	0.3900226144882737
1104	68-69	0.3852987098471772
1104	70-71	0.4498380931672372
1104	72-73	0.4115123870407871
1104	74-75	0.4361918300229135
1104	76-77	0.4165278872758691
1104	78-79	0.3647213706771453
1104	80-81	0.3640091658898541
1104	82-83	0.37148523662329325
1104	84-85	0.3390384305500618
1104	86-87	0.35017434038161355
1104	88-89	0.35603624294434155
1104	90-91	0.3562870538517089
1104	92-93	0.35832169239849776
1104	94-95	0.36133583052431817
1104	96-97	0.3735473362675208
1104	98-99	0.3648550574111411
1104	100	0.454713411104823
1105	1	0.11544871136249313
1105	2	-0.41508263739997275
1105	3	0.023349132310883647
1105	4	0.07773111394910615
1105	5	0.10539980001816929
1105	6	0.21555292341209764
1105	7	0.2088967770084338
1105	8	0.20615235136838095
1105	9	0.2747780288338646
1105	10-11	0.2954134904950152
1105	12-13	0.26458764126996925
1105	14-15	0.36020354125407295
1105	16-17	0.3491114739547001
1105	18-19	0.3902101572161314
1105	20-21	0.36138486586766305
1105	22-23	0.34831090841797163
1105	24-25	0.431441928595909
1105	26-27	0.366330018635864
1105	28-29	0.38467969346355346
1105	30-31	0.4028121428248781
1105	32-33	0.39320090174391
1105	34-35	0.42276314394254655
1105	36-37	0.36224032905104764
1105	38-39	0.4091805281622314
1105	40-41	0.3822002453932143
1105	42-43	0.40837851283423277
1105	44-45	0.4776128937828261
1105	46-47	0.3922197198183639
1105	48-49	0.4263755009371266
1105	50-51	0.4549596306542085
1105	52-53	0.4029388323308041
1105	54-55	0.3892717148021987
1105	56-57	0.44355955461107044
1105	58-59	0.4253065817616246
1105	60-61	0.3874408250381123
1105	62-63	0.4032034323383513
1105	64-65	0.49864714379572206
1105	66-67	0.4486011861143311
1105	68-69	0.4297124573860529
1105	70-71	0.32531716468469085
1105	72-73	0.33650112140113464
1105	74-75	0.25883713790057783
1105	76-77	0.30886618109538944
1105	78-79	0.21253680578180933
1105	80-81	0.23421080681175965
1105	82-83	0.2795319996635115
1105	84-85	0.30109426670231443
1105	86-87	0.24433626503950023
1105	88-89	0.25053242833805456
1105	90-91	0.2700062615709271
1105	92-93	0.26349933651324875
1105	94-95	0.25502263441772044
1105	96-97	0.2292599006005176
1105	98-99	0.22750259342165435
1105	100	0.2827225101301245
1106	1	0.31227389133170647
1106	2	0.33402755171249154
1106	3	0.28958161974741614
1106	4	0.18468888930803473
1106	5	0.2130224763325117
1106	6	0.19834305543476205
1106	7	0.23038782361543042
1106	8	0.23117491991845185
1106	9	0.2733415166336215
1106	10-11	0.2873481832410718
1106	12-13	0.3464727642428471
1106	14-15	0.4005088488182409
1106	16-17	0.3996021354620396
1106	18-19	0.41080282687947545
1106	20-21	0.43158092663526304
1106	22-23	0.4041212150688551
1106	24-25	0.32335171365701143
1106	26-27	0.35823889886688676
1106	28-29	0.42327719889675564
1106	30-31	0.4342313727707392
1106	32-33	0.42236898407191603
1106	34-35	0.4057660800664209
1106	36-37	0.43368734225173
1106	38-39	0.39862429332124805
1106	40-41	0.4240600112490469
1106	42-43	0.40211712850586423
1106	44-45	0.4069182617021383
1106	46-47	0.42267235261036973
1106	48-49	0.35826102660909953
1106	50-51	0.36128319967787803
1106	52-53	0.319344286487393
1106	54-55	0.3411843913422601
1106	56-57	0.37772936854519656
1106	58-59	0.37223081079420695
1106	60-61	0.3388576546984865
1106	62-63	0.36279158351894836
1106	64-65	0.3014618514008731
1106	66-67	0.2739042519868562
1106	68-69	0.23207666899021007
1106	70-71	0.2494056403612177
1106	72-73	0.30922072341112994
1106	74-75	0.27162276466493296
1106	76-77	0.25784825223551167
1106	78-79	0.20076654750651812
1106	80-81	0.22810930894014803
1106	82-83	0.23804283629623768
1106	84-85	0.21409043052110377
1106	86-87	0.22252174665320723
1106	88-89	0.21122349777461125
1106	90-91	0.2386561964380789
1106	92-93	0.24079675226884234
1106	94-95	0.25589780396149564
1106	96-97	0.24603779673537218
1106	98-99	0.21884096337723946
1106	100	0.3076190388340656
1107	1	0.07898055970241202
1107	2	-0.5275178739289821
1107	3	-0.013483095644790666
1107	4	0.043511695959487895
1107	5	0.10249207587983733
1107	6	0.14193626024757577
1107	7	0.20469298655498847
1107	8	0.17573657753900562
1107	9	0.20444098825972645
1107	10-11	0.21839404247502614
1107	12-13	0.1945551614953871
1107	14-15	0.25432022056620696
1107	16-17	0.30169242907984284
1107	18-19	0.3139684370631102
1107	20-21	0.26017910037214165
1107	22-23	0.2553547393369229
1107	24-25	0.24174775603956533
1107	26-27	0.34601626627821247
1107	28-29	0.24969248782723952
1107	30-31	0.31470991541864635
1107	32-33	0.30530695760291593
1107	34-35	0.33407385635436526
1107	36-37	0.3726058292727146
1107	38-39	0.386519866730751
1107	40-41	0.3681645495169832
1107	42-43	0.3338258017754612
1107	44-45	0.27946072620664353
1107	46-47	0.2913485874343351
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2216	82-83	-0.7615947771025731
2216	84-85	-0.873244924149482
2216	86-87	-0.8063655636479368
2216	88-89	-0.8270056972867792
2216	90-91	-0.9031532268044771
2216	92-93	-0.8150462915110168
2216	94-95	-0.8299275759067015
2216	96-97	-0.9019090051027092
2216	98-99	-0.9836709907124686
2216	100	-0.9340411071527086
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	7.0
13	63.0
14	245.0
15	708.0
16	1682.0
17	3407.0
18	6068.0
19	9464.0
20	13613.0
21	19623.0
22	26731.0
23	32026.0
24	34841.0
25	38319.0
26	44636.0
27	56118.0
28	70611.0
29	87896.0
30	109075.0
31	134324.0
32	166885.0
33	209923.0
34	285318.0
35	387024.0
36	574420.0
37	961530.0
38	803541.0
39	291257.0
40	553.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05186143886885	21.664669795289214	13.546638103967767	24.736830661874176
2	15.088483322844557	20.21678585731449	39.98542250700841	24.709308312832544
3	18.12508839000439	26.4123978966726	29.695746158095567	25.76676755522745
4	12.439104170066777	17.177780000645495	38.69867474101747	31.684441088270255
5	12.741627467650085	38.73270942916086	35.175860652502	13.349802450687045
6	29.946026373071128	38.891652738087174	17.591361107500564	13.570959781341136
7	26.208982138014758	32.16395359613904	23.86073349588491	17.766330769961293
8	26.215928375674384	35.19860354167315	19.550279273358363	19.0351888092941
9	26.123363582607613	14.402601242313345	21.447548600521017	38.02648657455802
10-11	25.73357312458466	25.819684698456168	27.377290435139194	21.06945174181997
12-13	26.01426527582249	23.575886654010425	27.826761728840303	22.583086341326783
14-15	23.332545298464204	24.58701976121399	25.411160816556695	26.669274123765106
16-17	21.961353955999005	27.098537400274044	26.826510442441425	24.113598201285534
18-19	21.418840928604514	27.254358897845083	29.15546125413967	22.171338919410736
20-21	22.93199045243167	26.430381780305012	28.36861458311999	22.26901318414333
22-23	23.393943942976133	25.98440990327101	28.071133148790146	22.55051300496271
24-25	22.870488666702823	26.134090602760562	28.818664727954413	22.176756002582206
26-27	22.825138896024182	26.337812110582252	27.74606910835944	23.090979885034127
28-29	21.979939184130025	26.955828858796245	28.394461947975152	22.66977000909858
30-31	22.89548393651863	26.480258641363946	28.200121650046956	22.424135772070468
32-33	23.325732875170026	26.714851466116478	27.370791448431913	22.58862421028158
34-35	23.220805367822063	26.507511685358782	28.182152806311095	22.089530140508064
36-37	22.935233020710715	26.052607009019862	27.920939826705894	23.091220143563532
38-39	23.0879820920879	25.994413617482458	28.665165339713933	22.25243895071571
40-41	22.609064280647154	25.36387560356072	29.121558845740175	22.905501270051946
42-43	22.811118778485437	25.601442966539288	28.183352130541607	23.404086124433668
44-45	22.963219854312854	26.481460038554548	27.928525791823727	22.626794315308867
46-47	23.035338011383296	26.288973324863292	27.189037216310076	23.486651447443336
48-49	23.889303468071184	25.991723367139652	27.305027834777647	22.813945330011514
50-51	23.19729779455513	26.084552732412003	27.074812948178362	23.643336524854504
52-53	22.586450253460484	26.62460937428488	26.980039872656477	23.808900499598163
54-55	22.692444071082186	26.598803931303227	26.79224087418944	23.91651112342515
56-57	23.831874721390015	25.968624511088105	26.57606292855593	23.62343783896595
58-59	22.573966416436235	26.415731415702236	27.579738280381576	23.430563887479945
60-61	22.768045882872403	27.071796338116382	26.731171435014843	23.428986343996367
62-63	23.24606307861577	26.249453078231323	26.299854550846792	24.204629292306116
64-65	22.34036978319014	26.44902231440816	26.773090625165906	24.437517277235788
66-67	22.988643707241703	26.04225210942463	26.78861725361975	24.180486929713915
68-69	22.44208588638363	25.97681455984404	27.20872056495245	24.37237898881988
70-71	22.405028965293138	25.673570357114663	27.307389096051725	24.614011581540478
72-73	23.047946960945666	25.68155834717038	27.63667780953026	23.633816882353695
74-75	23.18522063811692	26.122046831915892	27.12140505587708	23.571327474090108
76-77	22.877369015202294	26.1250226268885	26.614060914902076	24.38354744300713
78-79	22.58038015135847	26.213960485513255	26.81943325359832	24.386226109529964
80-81	22.195073670672585	26.629937170821904	27.42423254233332	23.750756616172193
82-83	23.096073555935455	25.510518738138167	28.381765071960796	23.01164263396558
84-85	23.460484077855785	26.02452268123741	26.85112991395916	23.66386332694765
86-87	21.941995247590555	26.686440528628314	27.386989529640537	23.984574694140594
88-89	22.86111935302154	25.536166946001636	27.20992254562169	24.392791155355138
90-91	22.449699068042925	25.476479889639403	27.483562566602487	24.59025847571519
92-93	22.81370196172397	25.82371477883475	27.145475752134175	24.217107507307105
94-95	21.580990013273908	25.76093850350258	27.88091949598733	24.777151987236177
96-97	21.976719305492466	25.621590881785195	27.414053319266817	24.987636493455522
98-99	21.34769300548385	26.38873521524462	27.296573631402865	24.96699814786866
100	22.160156012921682	25.589920997273442	26.525861111397393	25.724061878407483
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	87.0
1	64.0
2	69.5
3	92.5
4	67.0
5	59.0
6	114.0
7	318.5
8	521.0
9	609.5
10	517.0
11	365.5
12	392.5
13	486.5
14	610.0
15	801.5
16	1101.0
17	1427.0
18	1942.0
19	2594.5
20	3346.0
21	4151.0
22	4943.0
23	5930.0
24	6903.5
25	8015.0
26	9578.5
27	11307.5
28	14887.5
29	22404.5
30	32883.5
31	42769.0
32	57028.0
33	70839.5
34	81112.5
35	94066.5
36	105970.5
37	126692.0
38	136911.5
39	128148.0
40	119302.0
41	111300.5
42	110130.5
43	117242.5
44	129408.5
45	156605.5
46	182730.5
47	199190.5
48	210031.0
49	205073.0
50	195353.5
51	199417.5
52	203018.5
53	196238.0
54	189043.0
55	164745.0
56	143927.5
57	121157.5
58	95366.0
59	91549.0
60	75970.0
61	51137.5
62	37078.0
63	30107.0
64	21809.5
65	10694.5
66	5977.5
67	4380.0
68	3260.0
69	2331.0
70	1660.0
71	1240.5
72	984.0
73	791.5
74	617.5
75	450.5
76	258.0
77	122.5
78	63.5
79	29.0
80	18.0
81	7.5
82	3.5
83	1.5
84	2.0
85	1.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01320392721867168
2	0.003707168473873158
3	0.00169339794485564
4	0.027025715849655553
5	0.002677399453352836
6	0.0
7	3.89023852196566E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008993316112544144
16-17	0.0028490276234395566
18-19	0.0
20-21	8.009314604046947E-5
22-23	5.148845102601609E-4
24-25	2.2883756011562703E-5
26-27	1.4874441407515757E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.691169982370354E-4
36-37	0.0
38-39	0.0
40-41	2.7460507213875247E-4
42-43	5.83535778294849E-4
44-45	0.0
46-47	0.0
48-49	4.3479136421969137E-4
50-51	0.0
52-53	0.0
54-55	2.860469501445338E-4
56-57	9.153502404625081E-5
58-59	1.4874441407515757E-4
60-61	5.7209390028906755E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013158159706648555
70-71	6.17861412312193E-4
72-73	0.0
74-75	1.716281700867203E-4
76-77	0.00254009691728346
78-79	4.805588762428168E-4
80-81	0.0029062370134684636
82-83	0.0015446535307804825
84-85	0.005560752710809737
86-87	0.007757593287919757
88-89	0.007036754973555532
90-91	0.005412008296734579
92-93	0.00829536155419148
94-95	0.0014073509947111064
96-97	0.004508099934277853
98-99	0.0018650261149423604
100	0.08254170793370667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4369912.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.73773056471001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.8758537249608	15.874809970080777
2	16.1061748194176	7.646480765829039
3	6.548992653812283	4.6637466925938345
4	3.199914985163287	3.0383487899113653
5	1.8857436825901106	2.238163772571404
6	1.164084285109707	1.6579631468728377
7	0.7819527194337865	1.299324807778305
8	0.5200077790912849	0.9875043641297732
9	0.3785720421360191	0.808779702200118
>10	1.9152108599389828	8.314537742296512
>50	0.22543185114254047	3.798055241824848
>100	0.29025146156481413	15.647150001701995
>500	0.059434474419947744	9.947674358784447
>1k	0.04568183713505905	19.124569180505897
>5k	0.002115790351518524	3.099646430224129
>10k+	5.770337322323247E-4	1.8532450326947663
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	18836	0.4310384282337951	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13659	0.312569223361935	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13339	0.3052464214382349	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	12032	0.27533735233112244	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11822	0.2705317635686943	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11491	0.262957240328867	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9462	0.21652609938140632	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7779	0.17801273801394626	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6837	0.1564562398510542	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6815	0.15595279721879984	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6747	0.15439670181001355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6501	0.14876729783116915	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6423	0.14698236486226726	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6139	0.14048337815498343	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6019	0.13773732743359593	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5961	0.13641006958492527	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5947	0.1360896970007634	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5896	0.13492262544417372	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5875	0.13444206656793087	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5807	0.13288597115914463	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5803	0.1327944361350984	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5636	0.12897284888116742	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5489	0.1256089367474677	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5473	0.12524279665128268	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5456	0.1248537727990861	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5322	0.12178734949353671	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5251	0.12016260281671576	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5138	0.11757673838740917	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4918	0.11254231206486538	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4851	0.11100910041209068	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4772	0.10920128368717723	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4724	0.10810286339862221	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4659	0.10661541925787064	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4622	0.10576872028544282	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	4547	0.10405243858457561	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4475	0.1024048081517431	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4407	0.10084871274295684	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4386	0.10036815386671402	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	4375	0.10011643255058683	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	2.2883756011562703E-5	0.0	0.0	0.0	0.0
12-13	2.2883756011562703E-5	0.0	0.0	0.0	1.3730253606937623E-4
14-15	2.2883756011562703E-5	0.0	0.0	0.0	1.94511926098283E-4
16-17	2.2883756011562703E-5	0.0	0.0	0.0	2.2883756011562705E-4
18-19	2.2883756011562703E-5	0.0	0.0	0.0	2.7460507213875247E-4
20-21	2.2883756011562703E-5	0.0	0.0	1.1441878005781352E-5	2.860469501445338E-4
22-23	2.2883756011562703E-5	0.0	0.0	1.0297690205203218E-4	3.432563401734406E-4
24-25	3.432563401734406E-5	0.0	0.0	2.0595380410406435E-4	3.432563401734406E-4
26-27	4.576751202312541E-5	0.0	0.0	5.263263882659422E-4	3.5469821817922195E-4
28-29	4.576751202312541E-5	0.0	0.0	0.0015103278967631385	3.6614009618500325E-4
30-31	4.576751202312541E-5	0.0	0.0	0.006384567927225995	3.6614009618500325E-4
32-33	4.576751202312541E-5	0.0	0.0	0.017483189592833907	3.6614009618500325E-4
34-35	4.576751202312541E-5	0.0	0.0	0.035755868768066724	4.3479136421969137E-4
36-37	4.576751202312541E-5	0.0	0.0	0.06861694240067076	4.3479136421969137E-4
38-39	4.576751202312541E-5	0.0	0.0	0.12135255812931703	4.576751202312541E-4
40-41	4.576751202312541E-5	0.0	0.0	0.17959171717874411	4.576751202312541E-4
42-43	4.576751202312541E-5	0.0	0.0	0.24319711701288263	4.576751202312541E-4
44-45	4.576751202312541E-5	0.0	0.0	0.32058997984398774	4.6911699823703546E-4
46-47	4.576751202312541E-5	0.0	0.0	0.4194935733259617	4.8055887624281677E-4
48-49	4.576751202312541E-5	0.0	0.0	0.5343929122600181	5.034426322543795E-4
50-51	4.576751202312541E-5	0.0	0.0	0.6604023147376881	5.263263882659422E-4
52-53	4.576751202312541E-5	0.0	0.0	0.7831278982276988	5.720939002890676E-4
54-55	4.576751202312541E-5	0.0	0.0	0.9208881094173064	5.720939002890676E-4
56-57	4.576751202312541E-5	0.0	0.0	1.0955369352975528	5.720939002890676E-4
58-59	4.576751202312541E-5	0.0	0.0	1.3064107469441033	5.83535778294849E-4
60-61	4.576751202312541E-5	0.0	0.0	1.5230855907395846	5.949776563006303E-4
62-63	4.576751202312541E-5	0.0	0.0	1.7517858483191424	6.17861412312193E-4
64-65	4.576751202312541E-5	0.0	0.0	1.9975802716393374	6.52187046329537E-4
66-67	4.576751202312541E-5	0.0	0.0	2.27467967318335	6.750708023410999E-4
68-69	4.576751202312541E-5	0.0	0.0	2.5888049919540714	6.865126803468812E-4
70-71	4.576751202312541E-5	0.0	0.0	2.9421187428945936	6.865126803468812E-4
72-73	4.576751202312541E-5	0.0	0.0	3.3138081499124006	7.208383143642252E-4
74-75	4.576751202312541E-5	0.0	0.0	3.6718245127132993	7.78047704393132E-4
76-77	4.576751202312541E-5	0.0	0.0	4.090459945188828	8.009314604046947E-4
78-79	4.576751202312541E-5	0.0	0.0	4.546155620525082	8.238152164162574E-4
80-81	4.576751202312541E-5	0.0	0.0	5.043236568608247	8.352570944220387E-4
82-83	4.576751202312541E-5	0.0	0.0	5.566347789154564	9.153502404625082E-4
84-85	4.576751202312541E-5	0.0	0.0	6.079882157810043	9.153502404625082E-4
86-87	4.576751202312541E-5	0.0	0.0	6.631792585296912	9.72559630491415E-4
88	4.576751202312541E-5	0.0	0.0	7.0701423735764015	0.0010297690205203216
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8300	0.0	39.090153	1
GGGCACC	4895	0.0	37.47508	94
GTATCAA	13310	0.0	35.257282	1
GTACTAG	4480	0.0	32.642147	1
GTGGTAT	2275	0.0	31.416552	1
CTGTGCG	6460	0.0	30.04934	9
TAGTACT	6605	0.0	29.751942	4
CAACGCA	15240	0.0	29.019323	5
TATTCTC	2365	0.0	28.616333	5
ATCAACG	15625	0.0	28.312712	3
TACCCCG	320	0.0	27.905272	5
TATTCGC	490	0.0	27.81758	9
ACTGTGC	7620	0.0	27.507593	8
CTAGTAC	7505	0.0	26.998425	3
GTACTGT	7430	0.0	26.75619	6
TATCAAC	17545	0.0	26.742924	2
TAGGCAT	6240	0.0	26.587208	5
AGGGCTA	5230	0.0	26.060272	5
CTATTGA	5145	0.0	25.944803	9
TACTGTG	8265	0.0	25.758944	7
>>END_MODULE