##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146447_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5033122 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.958765354783772 31.0 30.0 34.0 26.0 34.0 2 30.088588553982994 31.0 30.0 34.0 26.0 34.0 3 30.28301141915495 31.0 30.0 34.0 26.0 34.0 4 33.73433745496334 35.0 35.0 37.0 28.0 37.0 5 33.95058613719278 35.0 35.0 37.0 28.0 37.0 6 34.08901115450808 37.0 35.0 37.0 29.0 37.0 7 33.928329573572825 36.0 35.0 37.0 28.0 37.0 8 33.89083276741553 36.0 35.0 37.0 28.0 37.0 9 35.2125400496948 38.0 35.0 39.0 28.0 39.0 10-11 35.12563742742576 38.0 35.0 39.0 27.5 39.0 12-13 35.10083880343055 38.0 35.0 39.0 27.0 39.0 14-15 36.03374813882914 39.0 35.0 41.0 27.0 41.0 16-17 35.858423857001675 39.0 34.5 41.0 27.0 41.0 18-19 35.80528963931333 39.0 34.0 41.0 27.0 41.0 20-21 36.125889755901014 39.0 35.0 41.0 27.0 41.0 22-23 35.84311109486319 39.0 34.5 41.0 26.5 41.0 24-25 35.92639111469978 39.0 34.0 41.0 27.0 41.0 26-27 35.977704593689566 39.0 34.5 41.0 27.0 41.0 28-29 35.81244752263108 39.0 34.0 41.0 26.5 41.0 30-31 35.742497400221964 39.0 34.0 41.0 26.5 41.0 32-33 35.8277035605336 39.0 34.0 41.0 27.0 41.0 34-35 35.8675864602527 39.0 34.0 41.0 27.0 41.0 36-37 35.79242704627465 39.0 34.0 41.0 27.0 41.0 38-39 35.634561808754086 39.0 34.0 41.0 26.0 41.0 40-41 35.59557944353425 38.5 34.0 40.5 26.0 41.0 42-43 35.438397777761 38.0 34.0 40.0 26.0 41.0 44-45 35.25907418894277 38.0 33.0 40.0 26.0 41.0 46-47 35.13317529358517 38.0 33.0 40.0 25.5 41.0 48-49 35.002684616029576 38.0 33.0 40.0 25.0 41.0 50-51 34.798374249620814 38.0 33.0 40.0 24.5 41.0 52-53 34.614314634137614 37.5 33.0 40.0 24.0 41.0 54-55 34.42201301697038 37.0 32.5 40.0 24.0 41.0 56-57 34.27110578682576 37.0 32.0 40.0 24.0 41.0 58-59 34.01104771551336 36.0 32.0 40.0 24.0 41.0 60-61 33.76721843817813 36.0 32.0 39.0 24.0 41.0 62-63 33.50000218552223 35.0 31.5 39.0 23.0 41.0 64-65 33.21637216423524 35.0 31.0 39.0 23.0 41.0 66-67 32.93722464903493 35.0 31.0 38.0 23.0 40.0 68-69 32.65312126350206 35.0 31.0 38.0 22.0 40.0 70-71 32.60643582651086 35.0 31.0 37.0 23.0 40.0 72-73 32.406436601377834 35.0 31.0 37.0 23.0 40.0 74-75 32.14831778367383 35.0 30.5 36.0 22.5 39.0 76-77 31.921976558485966 35.0 30.0 36.0 22.0 39.0 78-79 31.707443709888217 34.5 30.0 36.0 22.0 39.0 80-81 31.49433989480088 34.0 30.0 35.0 21.5 37.5 82-83 31.260276623535056 34.0 30.0 35.0 20.5 37.0 84-85 31.076396816925957 34.0 30.0 35.0 20.0 36.5 86-87 30.921646246603995 34.0 30.0 35.0 20.0 36.0 88-89 30.934233066474448 34.0 30.0 35.0 20.0 36.0 90-91 30.858167952217332 34.0 30.0 35.0 20.0 36.0 92-93 30.718713255907566 34.0 30.0 35.0 20.0 35.5 94-95 30.692100549122394 34.0 30.0 35.0 20.0 35.0 96-97 30.60604432000655 34.0 30.0 35.0 20.0 35.0 98-99 30.533557104318156 34.0 30.0 35.0 19.5 35.0 100 30.008799309851817 34.0 29.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8813547265204704 1101 2 1.0079415750104346 1101 3 0.9907059273767977 1101 4 1.0066833928916594 1101 5 0.9969782908717022 1101 6 0.7126594536309057 1101 7 0.7977661858142824 1101 8 0.9237003877049759 1101 9 1.0677143265913074 1101 10-11 1.0759787526902258 1101 12-13 0.9186381923057283 1101 14-15 1.1802707263492422 1101 16-17 1.2629218572387089 1101 18-19 1.477380630067536 1101 20-21 1.117268662344621 1101 22-23 1.4495617934438556 1101 24-25 1.3778799132612392 1101 26-27 1.2119728135351266 1101 28-29 1.3948515677843076 1101 30-31 1.3659820070900395 1101 32-33 1.175420981889097 1101 34-35 1.1057000325025683 1101 36-37 1.087326312883448 1101 38-39 1.1874108049000256 1101 40-41 1.1217801203008264 1101 42-43 1.1753816810670514 1101 44-45 1.172339216826117 1101 46-47 1.1729309352620305 1101 48-49 1.1392963350784981 1101 50-51 1.1651609339793723 1101 52-53 1.1381256253060599 1101 54-55 1.1656692055525752 1101 56-57 0.9872728904792822 1101 58-59 0.9732224461831649 1101 60-61 0.9598598721641807 1101 62-63 0.9201983146346251 1101 64-65 0.9215773864136523 1101 66-67 0.9183763470552293 1101 68-69 0.9491262641723637 1101 70-71 0.8804468758103781 1101 72-73 0.8432937028899303 1101 74-75 0.8958408838578222 1101 76-77 0.9524219407143946 1101 78-79 0.8951008421477908 1101 80-81 0.8687419962907121 1101 82-83 0.8670398426113657 1101 84-85 0.8851548341026572 1101 86-87 0.8203345749870934 1101 88-89 0.9172427136820573 1101 90-91 0.9535931855690016 1101 92-93 0.95665803282429 1101 94-95 0.8797316188005979 1101 96-97 0.8998273259013985 1101 98-99 0.9132165099397689 1101 100 1.0062552541217862 1102 1 0.7341929518848289 1102 2 0.8518916195854409 1102 3 0.8772624777979559 1102 4 0.945653418482614 1102 5 0.8205890978882593 1102 6 0.6139462865164589 1102 7 0.6412166114914584 1102 8 0.6204144594628858 1102 9 0.7705281166445701 1102 10-11 0.8472733703073914 1102 12-13 0.7932696991589907 1102 14-15 1.0409621981024273 1102 16-17 1.1364459478855693 1102 18-19 1.1880988438871611 1102 20-21 0.9482412579842787 1102 22-23 1.2370896040709738 1102 24-25 1.0614405040705748 1102 26-27 0.9029423899566069 1102 28-29 1.0752529522969922 1102 30-31 1.1038166642631921 1102 32-33 1.0312685746302819 1102 34-35 0.9226111310865903 1102 36-37 0.8978739256494563 1102 38-39 0.907288513741392 1102 40-41 0.9029109858039845 1102 42-43 0.9382520401878551 1102 44-45 1.0104011715462846 1102 46-47 0.9514406949074754 1102 48-49 0.8784943487738133 1102 50-51 0.9787563622707722 1102 52-53 0.9077673799232855 1102 54-55 1.0211341467181612 1102 56-57 0.8767207857513597 1102 58-59 0.8370306623034054 1102 60-61 0.8264389128488787 1102 62-63 0.8459799974851663 1102 64-65 0.7257918070566163 1102 66-67 0.8730549758311597 1102 68-69 0.8950860723464942 1102 70-71 0.7672245181124708 1102 72-73 0.7391826481275814 1102 74-75 0.7765534492657551 1102 76-77 0.7228273154997567 1102 78-79 0.7529229413288192 1102 80-81 0.8274769615085269 1102 82-83 0.7690471859205701 1102 84-85 0.7587969739894262 1102 86-87 0.7980094775316857 1102 88-89 0.7331939242170336 1102 90-91 0.7356145427842371 1102 92-93 0.8012636193342679 1102 94-95 0.782613545624919 1102 96-97 0.8047959981068473 1102 98-99 0.8306462691727461 1102 100 0.8845244487038002 1103 1 0.6631578534223763 1103 2 0.788654196906549 1103 3 0.7560545206476306 1103 4 0.7951074370726587 1103 5 0.8003664096831429 1103 6 0.5957296643218228 1103 7 0.6591300253146812 1103 8 0.6528693877848823 1103 9 0.6908478467537265 1103 10-11 0.8347274711936095 1103 12-13 0.7813859757163826 1103 14-15 0.9279253813568076 1103 16-17 1.1424771674631486 1103 18-19 1.1658407077213013 1103 20-21 0.9344357813730539 1103 22-23 1.1574370533791267 1103 24-25 1.0111031331720284 1103 26-27 0.9352002121362659 1103 28-29 1.084441936880971 1103 30-31 1.1167202084889851 1103 32-33 0.9256392013022747 1103 34-35 0.9221314507151988 1103 36-37 0.9287881713424682 1103 38-39 1.0096438964165202 1103 40-41 0.9359397970365819 1103 42-43 0.8736567774210044 1103 44-45 0.9827569833314413 1103 46-47 0.9602900375017001 1103 48-49 0.9670375704440275 1103 50-51 1.0046678100675734 1103 52-53 0.9534029442953269 1103 54-55 0.9750061141486484 1103 56-57 0.8543599797024299 1103 58-59 0.8239371170250678 1103 60-61 0.841231629667071 1103 62-63 0.7822489647193649 1103 64-65 0.7779961938859685 1103 66-67 0.8114754564074147 1103 68-69 0.8423734512322625 1103 70-71 0.7371200322913296 1103 72-73 0.7758819794651757 1103 74-75 0.7483759652979884 1103 76-77 0.7573559283841647 1103 78-79 0.7974933165153502 1103 80-81 0.8389677547879337 1103 82-83 0.7705509274247966 1103 84-85 0.7712779990925505 1103 86-87 0.8580235800664937 1103 88-89 0.8266280653300768 1103 90-91 0.8060372012040666 1103 92-93 0.8652976534622816 1103 94-95 0.856776112296405 1103 96-97 0.8670252125506224 1103 98-99 0.8722191846025034 1103 100 0.9275421347280393 1104 1 0.6823181221754204 1104 2 0.7775801848096648 1104 3 0.7678502722481291 1104 4 0.84022356966193 1104 5 0.7610781947134129 1104 6 0.5516198015569529 1104 7 0.5613110424433003 1104 8 0.5947350472643791 1104 9 0.7522821597634888 1104 10-11 0.773173203149419 1104 12-13 0.6928296444289757 1104 14-15 0.9325315147208428 1104 16-17 1.0216099468152677 1104 18-19 1.0229953662675229 1104 20-21 0.8536271194015157 1104 22-23 1.0834960994693645 1104 24-25 0.9365878390194524 1104 26-27 0.8431526509940142 1104 28-29 1.0213003033742822 1104 30-31 1.1088744716194086 1104 32-33 0.8971485607257605 1104 34-35 0.7489492156892723 1104 36-37 0.7848471186307293 1104 38-39 0.860199850205511 1104 40-41 0.8062197521186789 1104 42-43 0.8134373477706447 1104 44-45 0.9518552555701589 1104 46-47 0.8406208844754204 1104 48-49 0.7673715876727911 1104 50-51 0.863103783491141 1104 52-53 0.9188597536166796 1104 54-55 0.8513593774500734 1104 56-57 0.7291774125466262 1104 58-59 0.7484652866146178 1104 60-61 0.7917056581792181 1104 62-63 0.7080910136558387 1104 64-65 0.7100175657117802 1104 66-67 0.8399794830595084 1104 68-69 0.78882964777344 1104 70-71 0.676692557252025 1104 72-73 0.6492020079262488 1104 74-75 0.7000957302723165 1104 76-77 0.7286642195295343 1104 78-79 0.6440986804987503 1104 80-81 0.7219213233556339 1104 82-83 0.6932960329469111 1104 84-85 0.6391140877333683 1104 86-87 0.6499403401273653 1104 88-89 0.6277621222902674 1104 90-91 0.6469848142430017 1104 92-93 0.6730673031022611 1104 94-95 0.6254061171626475 1104 96-97 0.5866943226195147 1104 98-99 0.7061993453022062 1104 100 0.7684800130691478 1105 1 0.4683611215314123 1105 2 0.586516957409156 1105 3 0.5410533717783572 1105 4 0.7036844282971302 1105 5 0.5540933676244606 1105 6 0.2572425066983399 1105 7 0.30548527184541996 1105 8 0.42224654566471287 1105 9 0.41904981707625666 1105 10-11 0.5153692464462196 1105 12-13 0.4161569645755563 1105 14-15 0.6368278265382017 1105 16-17 0.804394385378771 1105 18-19 0.7616713551346166 1105 20-21 0.5729912356173088 1105 22-23 0.8639780998174018 1105 24-25 0.6705123028535382 1105 26-27 0.5321290636950948 1105 28-29 0.731466102433707 1105 30-31 0.7558365478071778 1105 32-33 0.5773093811160663 1105 34-35 0.5319013920780122 1105 36-37 0.45098347832274754 1105 38-39 0.5563364360656564 1105 40-41 0.4155862993235928 1105 42-43 0.524446079260656 1105 44-45 0.49862222037563697 1105 46-47 0.4348008147570752 1105 48-49 0.5006739618567977 1105 50-51 0.6148969721303388 1105 52-53 0.5468778516496613 1105 54-55 0.4640081843028767 1105 56-57 0.4976038030318861 1105 58-59 0.5418859284831541 1105 60-61 0.5255078511953428 1105 62-63 0.4807293293626955 1105 64-65 0.41872820837339475 1105 66-67 0.47492172044107406 1105 68-69 0.5775247548487741 1105 70-71 0.473908750092221 1105 72-73 0.377771485183807 1105 74-75 0.4192011534318141 1105 76-77 0.4362587246030216 1105 78-79 0.42845640905618154 1105 80-81 0.4878713001426327 1105 82-83 0.45258964411315006 1105 84-85 0.43312620381032474 1105 86-87 0.5026669751245549 1105 88-89 0.5419293235072118 1105 90-91 0.5844036053518664 1105 92-93 0.5982675414571581 1105 94-95 0.5100584625918998 1105 96-97 0.5155271690548773 1105 98-99 0.5563712776338896 1105 100 0.6149892098587131 1106 1 0.5450106735418778 1106 2 0.5684409612518309 1106 3 0.6273924313484578 1106 4 0.7129399104035059 1106 5 0.6202173382332674 1106 6 0.40792986005497056 1106 7 0.3766132460141236 1106 8 0.4660583374321874 1106 9 0.5663531286733274 1106 10-11 0.5403555162316778 1106 12-13 0.45914882099268794 1106 14-15 0.6733967489304078 1106 16-17 0.7690902851378922 1106 18-19 0.7481663082498002 1106 20-21 0.6024839578359504 1106 22-23 0.9233493613197368 1106 24-25 0.6913816719749093 1106 26-27 0.5614674562313411 1106 28-29 0.8175225455944002 1106 30-31 0.7977334147046804 1106 32-33 0.6488457590972416 1106 34-35 0.6004758426891215 1106 36-37 0.5742695151640689 1106 38-39 0.6945363030016551 1106 40-41 0.6317537046118957 1106 42-43 0.6103620032889197 1106 44-45 0.6030706670708099 1106 46-47 0.6603801455480962 1106 48-49 0.6491712576240118 1106 50-51 0.6368568248473281 1106 52-53 0.5779294450507848 1106 54-55 0.6694119225916708 1106 56-57 0.6072883547892047 1106 58-59 0.6137203686956525 1106 60-61 0.6078234348299745 1106 62-63 0.5790909225517353 1106 64-65 0.5808953992911086 1106 66-67 0.5601115123166593 1106 68-69 0.49839877271265465 1106 70-71 0.5428189884441821 1106 72-73 0.44929758023224764 1106 74-75 0.5341217888285144 1106 76-77 0.5557283937717941 1106 78-79 0.5444851804163875 1106 80-81 0.5868515974007984 1106 82-83 0.564949907804678 1106 84-85 0.5125409221290163 1106 86-87 0.5636610976429814 1106 88-89 0.5745969369177857 1106 90-91 0.5288093864452215 1106 92-93 0.536452969441747 1106 94-95 0.576717302804191 1106 96-97 0.5119192449983316 1106 98-99 0.5227840843982285 1106 100 0.5382815307144924 1107 1 0.4743838422835438 1107 2 0.5650541589119982 1107 3 0.48757433252338345 1107 4 0.6488704986014255 1107 5 0.5437848464043498 1107 6 0.2817446629437228 1107 7 0.34710183101503134 1107 8 0.39023787293528045 1107 9 0.5266339477353199 1107 10-11 0.49954860808332313 1107 12-13 0.38745273278683356 1107 14-15 0.6141510960691576 1107 16-17 0.6886680744016402 1107 18-19 0.7871500614541134 1107 20-21 0.567002874291731 1107 22-23 0.8191401241558083 1107 24-25 0.4389823180290193 1107 26-27 0.4131293854662772 1107 28-29 0.5278338416560828 1107 30-31 0.5787710924063418 1107 32-33 0.5843089480197463 1107 34-35 0.48315282550807126 1107 36-37 0.3934012678342427 1107 38-39 0.46180129058237895 1107 40-41 0.3587280559620609 1107 42-43 0.4916482229306922 1107 44-45 0.4367876806133708 1107 46-47 0.4610293408195716 1107 48-49 0.437564548718548 1107 50-51 0.5140314886498061 1107 52-53 0.4576725840303837 1107 54-55 0.432121300503411 1107 56-57 0.4101134593089384 1107 58-59 0.4558614353103394 1107 60-61 0.497860103169792 1107 62-63 0.480343894299061 1107 64-65 0.5275372475340561 1107 66-67 0.48156221039523217 1107 68-69 0.4903290587345168 1107 70-71 0.42876161180566896 1107 72-73 0.4010228442045616 1107 74-75 0.46574897634474866 1107 76-77 0.42749162589581147 1107 78-79 0.36311930199192943 1107 80-81 0.48974744181907326 1107 82-83 0.4355119403494392 1107 84-85 0.39559404023131606 1107 86-87 0.4792602259943948 1107 88-89 0.4486426571787945 1107 90-91 0.47855219212150146 1107 92-93 0.5721886356045758 1107 94-95 0.4778329095224798 1107 96-97 0.4414448946786358 1107 98-99 0.453261375462354 1107 100 0.5273738420180898 1108 1 0.304155171275589 1108 2 0.48442364922621906 1108 3 0.46442088621923716 1108 4 0.5382562031471849 1108 5 0.4301463058385835 1108 6 0.17771384892609632 1108 7 0.21679135590177623 1108 8 0.257053649174388 1108 9 0.37201815674499983 1108 10-11 0.4256118535627067 1108 12-13 0.22417364939925477 1108 14-15 0.4167484640663517 1108 16-17 0.5768162306231659 1108 18-19 0.6503280661169555 1108 20-21 0.3472840632169678 1108 22-23 0.5704703938142117 1108 24-25 0.4448768589763148 1108 26-27 0.3710280393396346 1108 28-29 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-0.7932657588846261 2216 4 -0.7911208861636752 2216 5 -0.8596936330079785 2216 6 -0.7903811059238279 2216 7 -1.0296578110771932 2216 8 -0.9755631165549659 2216 9 -0.9984683528143634 2216 10-11 -1.0202657562221944 2216 12-13 -1.3081883871018576 2216 14-15 -1.7360919139395818 2216 16-17 -1.402754550272114 2216 18-19 -3.4139128217994283 2216 20-21 -1.7111622733750593 2216 22-23 -1.1386999552567971 2216 24-25 -1.199708693721817 2216 26-27 -1.2295134908376468 2216 28-29 -1.1208427891244312 2216 30-31 -1.316815846435773 2216 32-33 -1.3525076664654634 2216 34-35 -1.553871794497681 2216 36-37 -1.5452587232342196 2216 38-39 -1.6565376557362868 2216 40-41 -1.7576193307890051 2216 42-43 -1.6155457771680588 2216 44-45 -1.5159885449262163 2216 46-47 -1.606243470181063 2216 48-49 -1.619526060192598 2216 50-51 -1.5815691107944048 2216 52-53 -1.527263340428341 2216 54-55 -1.4581078755170083 2216 56-57 -1.5131560802714787 2216 58-59 -1.6440312562181845 2216 60-61 -1.6639042792543748 2216 62-63 -1.638174929489196 2216 64-65 -1.6552014272291764 2216 66-67 -1.5355262779681595 2216 68-69 -1.6917642030071889 2216 70-71 -1.7742737756986813 2216 72-73 -1.756052728602441 2216 74-75 -1.7351443403397901 2216 76-77 -1.609128025710767 2216 78-79 -1.7191090894129424 2216 80-81 -1.7181431459853052 2216 82-83 -1.9477232477307247 2216 84-85 -1.7188812367504802 2216 86-87 -1.7268002037798205 2216 88-89 -1.6068693002792642 2216 90-91 -1.715683136792041 2216 92-93 -1.5515957430140972 2216 94-95 -1.4882137776774371 2216 96-97 -1.462674191008638 2216 98-99 -1.4241874768378473 2216 100 -1.4798532302138163 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 20740.0 1 3188.0 2 530.0 3 484.0 4 430.0 5 297.0 6 243.0 7 320.0 8 524.0 9 1027.0 10 2322.0 11 5692.0 12 10632.0 13 15191.0 14 19274.0 15 22973.0 16 26890.0 17 29933.0 18 33763.0 19 37495.0 20 41825.0 21 46128.0 22 52213.0 23 58605.0 24 64722.0 25 72515.0 26 81459.0 27 92532.0 28 106253.0 29 123836.0 30 145028.0 31 171683.0 32 204474.0 33 254721.0 34 338622.0 35 441597.0 36 636571.0 37 906694.0 38 731849.0 39 229607.0 40 240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.47562972227746 21.09773053764006 12.280304296621582 24.146335443460895 2 15.341898431009335 19.916157117395002 40.885439274080134 23.85650517751553 3 18.473775318776166 26.849846049543242 28.836997779432107 25.83938085224849 4 12.004662818909567 16.679338382724413 40.656926511698465 30.65907228666755 5 12.943003080493916 38.67634141562244 35.338141974094164 13.042513529789481 6 29.514887649870715 38.77359161059408 18.41385398715588 13.297666752379325 7 26.671693119104763 31.749654714265947 24.112734968514456 17.46591719811484 8 25.812932804051087 35.34380715808979 20.249998003210838 18.593262034648287 9 26.153227991926354 14.882830434391014 21.81516918657004 37.148772387112594 10-11 25.577606453145695 25.927129874330756 27.76303911907994 20.732224553443608 12-13 25.71279344686312 23.794537887507147 27.718201132967664 22.774467532662072 14-15 23.135049825775308 24.962808797825158 25.115248537691958 26.786892838707573 16-17 22.11632432579264 27.440056309030158 26.498939312199738 23.944680052977464 18-19 21.510356008450266 28.353466659822512 28.014201749772855 22.121975581954366 20-21 23.24462559066398 27.42413531147242 26.875417123590616 22.45582197427298 22-23 23.729846027785197 27.032028728382535 26.58787964459288 22.650245599239383 24-25 23.09658762382753 27.09514919821054 27.42601505173998 22.38224812622195 26-27 23.022189766504543 27.330586445398016 26.352751450772576 23.29447233732487 28-29 22.14418121427584 27.924819560054537 26.952398402213458 22.97860082345617 30-31 23.019047772154856 27.5135507396228 26.88855367279139 22.57884781543095 32-33 23.404268491479144 27.712482194647887 25.927847945163755 22.955401368709214 34-35 23.380017422770223 27.4322945217142 26.930733253353306 22.256954802162277 36-37 23.022374329431347 27.067890493996394 26.606258387726623 23.303476788845636 38-39 23.24071695630341 27.117565445413117 27.235404940809598 22.406312657473872 40-41 22.80092063148992 26.25160078775437 27.8129468897026 23.134531691053105 42-43 22.77959839671897 26.50445424644553 27.117669689431217 23.59827766740428 44-45 22.91089392743042 27.501586925828757 26.96839982440185 22.61911932233897 46-47 22.83332807280465 27.135987729517197 26.546026096750314 23.484658100927838 48-49 23.58224938231243 26.7131711869557 26.843194382630475 22.861385048101393 50-51 23.14316374718426 26.581386720437276 26.764700154450683 23.510749377927784 52-53 22.54742756354608 27.166058213203016 26.64103429332733 23.645479929923578 54-55 22.53659683423514 27.05619462835286 26.668656143413312 23.73855239399869 56-57 23.633151172038367 26.524241060069286 26.393122475742565 23.44948529214978 58-59 22.543894366103643 26.891321852803586 27.35020149698886 23.214582284103912 60-61 22.664161209905135 27.529230439841673 26.572537946345655 23.234070403907538 62-63 23.097304262862455 26.852932967797244 26.074842366305305 23.974920403034993 64-65 22.216208027146166 26.996407894383367 26.62909814270893 24.15828593576154 66-67 23.017144941172806 26.49515667046085 26.591016626844024 23.896681761522316 68-69 22.33513358018057 26.643360345359728 26.879426744403233 24.142079330056465 70-71 22.290874961400934 26.349409319462037 27.00983781801535 24.34987790112168 72-73 22.924185219796676 26.383601037519135 27.447206960818182 23.245006781866007 74-75 23.066770171603075 26.737620079558184 27.002324666542627 23.19328508229611 76-77 22.72351304609369 26.66120239696901 26.620110529633727 23.995174027303573 78-79 22.570945025689813 26.603042382571385 26.721180457782793 24.104832133956013 80-81 22.151614185990447 26.91296304026482 27.43389176418518 23.50153100955955 82-83 22.97495769526711 25.8814788406431 28.457631104726495 22.68593235936329 84-85 23.229183301678745 26.455174678487975 26.998131485532422 23.317510534300858 86-87 21.819051002214543 27.082703461784053 27.5472907058308 23.5509548301706 88-89 22.953245715380874 25.756763049628677 27.376923736782928 23.91306749820752 90-91 22.599401577280066 25.848653548880147 27.57096439544511 23.98098047839468 92-93 22.864776323244246 26.06166222720298 27.459086410902266 23.6144750386505 94-95 21.708104485698232 26.053134368055098 28.13952572719159 24.09923541905508 96-97 22.053940514722477 25.992843270763256 27.65710571376317 24.296110500751094 98-99 21.403354144795916 26.653940832402935 27.5662580131931 24.37644700960805 100 22.25703868416671 25.82797445251443 26.772417569108427 25.142569294210443 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17125.0 1 10045.0 2 2688.5 3 1843.0 4 916.0 5 449.5 6 329.5 7 315.5 8 295.0 9 304.5 10 312.5 11 284.0 12 260.5 13 254.5 14 285.5 15 378.0 16 441.5 17 477.0 18 605.5 19 778.5 20 1049.0 21 1381.0 22 1894.5 23 2671.0 24 3715.0 25 5234.5 26 7463.5 27 10241.0 28 15069.5 29 24830.5 30 38844.0 31 52670.0 32 69882.0 33 86471.5 34 100131.0 35 115941.5 36 130296.5 37 153843.0 38 165296.0 39 156561.5 40 147253.0 41 136756.5 42 134438.0 43 143480.5 44 157737.5 45 184803.5 46 212628.0 47 232804.5 48 245222.0 49 240928.0 50 229663.5 51 231774.0 52 232407.5 53 221139.5 54 206570.5 55 177352.0 56 153585.5 57 126055.5 58 98171.0 59 91703.0 60 76000.0 61 52576.5 62 38778.0 63 32031.0 64 23612.0 65 11916.5 66 6238.5 67 3988.0 68 2820.0 69 1965.0 70 1399.0 71 1048.0 72 748.5 73 566.5 74 423.0 75 303.5 76 229.5 77 157.0 78 113.5 79 89.5 80 63.0 81 46.0 82 31.5 83 32.5 84 31.5 85 20.0 86 15.5 87 15.5 88 14.5 89 8.5 90 7.5 91 7.0 92 4.0 93 3.5 94 4.5 95 3.5 96 3.5 97 3.0 98 1.0 99 2.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9873593368092409 2 0.4434821965372586 3 0.47769555357489846 4 0.4624763715244733 5 0.42891867115480214 6 0.4795830500432932 7 0.4957360461359768 8 0.4983388044239739 9 0.5110347017219133 10-11 0.5254690826091639 12-13 0.523720664827914 14-15 0.5210583013882835 16-17 0.5258167793270261 18-19 0.524823360133134 20-21 0.5237802699795475 22-23 0.48788803450423013 24-25 0.5217238922481912 26-27 0.5021336657446412 28-29 0.5080246415644206 30-31 0.5113823984397755 32-33 0.5135877890502157 34-35 0.5256280296801865 36-37 0.5423274063295107 38-39 0.5312011113579206 40-41 0.5363072860145254 42-43 0.5342211057073523 44-45 0.5438870744639213 46-47 0.5421684592584881 48-49 0.5153560752153434 50-51 0.5298798638300443 52-53 0.5225881669468772 54-55 0.5320852544404844 56-57 0.5206311311349099 58-59 0.5259459238222319 60-61 0.5202337634573532 62-63 0.5138858148083834 64-65 0.5254889509930417 66-67 0.5233133629584182 68-69 0.5429433262297237 70-71 0.5269890139758187 72-73 0.5316084132274164 74-75 0.5284493401908399 76-77 0.5175415974419059 78-79 0.5471454894198869 80-81 0.5224391540677933 82-83 0.5224292198758543 84-85 0.5312507823176151 86-87 0.8349688324662109 88-89 1.10021175723537 90-91 1.0901484208012442 92-93 1.0898901318108323 94-95 1.094767820052842 96-97 1.0874065838261024 98-99 1.0835322489699235 100 1.07915127032486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5033122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.043852852975462 #Duplication Level Percentage of deduplicated Percentage of total 1 74.83133515083028 23.230529572140657 2 12.256797395462414 7.609964295869361 3 4.93063546750755 4.591977657748987 4 2.493801868211886 3.096688729649804 5 1.4330008663140554 2.224293401601995 6 0.9241257182276665 1.7213053688585964 7 0.6048582284096047 1.3143990887761432 8 0.40699176907264656 1.010767407317073 9 0.28276861402556386 0.7900404520724476 >10 1.420057915647643 7.9282517349300825 >50 0.15192023419921502 3.354814378401292 >100 0.19241381295488 13.38866241221202 >500 0.03827036766132185 8.264226452202072 >1k 0.03103867609608853 16.654430929515645 >5k 0.0014719372169279096 2.711557439139595 >10k+ 5.119781624097077E-4 2.108090679564368 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 18356 0.3647040544616244 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 15410 0.30617179555750884 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 13590 0.2700113368998407 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 12995 0.2581896484925261 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 12434 0.2470434851370581 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 12009 0.23859942198897624 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 11151 0.2215523486217898 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 10164 0.2019422537343621 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 7999 0.15892720263883928 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7335 0.14573459574395375 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 7179 0.14263512785901078 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6804 0.1351844839048209 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6687 0.13285988299111368 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6368 0.12652186853408282 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6340 0.1259655537855033 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6189 0.12296542781994953 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5998 0.11917056649928216 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5881 0.11684596558557492 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5665 0.11255439466796155 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5658 0.11241531598081668 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5613 0.11152123870631389 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5570 0.11066689819956678 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5546 0.11019005698649864 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5435 0.10798466637605844 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5320 0.10569980223010687 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5218 0.10367322707456725 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5207 0.10345467485191101 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5139 0.1021036247482179 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 5121 0.1017459938384168 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5109 0.10150757323188271 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5103 0.10138836292861568 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 1.986838387783964E-5 8 0.0 0.0 0.0 0.0 1.986838387783964E-5 9 0.0 0.0 0.0 0.0 1.986838387783964E-5 10-11 0.0 0.0 0.0 0.0 1.986838387783964E-5 12-13 0.0 1.986838387783964E-5 0.0 0.0 4.9670959694599096E-5 14-15 0.0 1.986838387783964E-5 0.0 0.0 5.9605151633518915E-5 16-17 0.0 1.986838387783964E-5 0.0 0.0 5.9605151633518915E-5 18-19 0.0 1.986838387783964E-5 0.0 0.0 9.934191938919819E-5 20-21 0.0 1.986838387783964E-5 0.0 0.0 9.934191938919819E-5 22-23 0.0 1.986838387783964E-5 0.0 7.947353551135855E-5 1.2914449520595766E-4 24-25 0.0 1.986838387783964E-5 0.0 2.18552222656236E-4 1.589470710227171E-4 26-27 0.0 1.986838387783964E-5 0.0 6.059857082741089E-4 1.589470710227171E-4 28-29 0.0 1.986838387783964E-5 0.0 0.0017484177812498883 1.589470710227171E-4 30-31 0.0 1.986838387783964E-5 0.0 0.007500314913884464 1.589470710227171E-4 32-33 0.0 1.986838387783964E-5 0.0 0.022183050599607956 1.9868383877839638E-4 34-35 0.0 1.986838387783964E-5 0.0 0.0443064960475824 1.9868383877839638E-4 36-37 0.0 1.986838387783964E-5 0.0 0.08205642541547771 1.9868383877839638E-4 38-39 0.0 1.986838387783964E-5 0.0 0.14372788897229194 1.9868383877839638E-4 40-41 0.0 1.986838387783964E-5 0.0 0.21249236557349493 2.1855222265623604E-4 42-43 0.0 1.986838387783964E-5 0.0 0.2873365676413169 2.1855222265623604E-4 44-45 0.0 1.986838387783964E-5 0.0 0.37679396605128984 2.2848641459515586E-4 46-47 0.0 1.986838387783964E-5 0.0 0.4913252649150964 2.3842060653407566E-4 48-49 0.0 1.986838387783964E-5 0.0 0.6237083066931419 2.3842060653407566E-4 50-51 0.0 1.986838387783964E-5 0.0 0.7682507994044253 2.582889904119153E-4 52-53 0.0 1.986838387783964E-5 0.0 0.9123661218623351 3.178941420454342E-4 54-55 0.0 1.986838387783964E-5 0.0 1.0705283917218775 3.3776252592327387E-4 56-57 0.0 1.986838387783964E-5 0.0 1.2650895408456222 3.3776252592327387E-4 58-59 0.0 1.986838387783964E-5 0.0 1.498274828227887 3.3776252592327387E-4 60-61 0.0 1.986838387783964E-5 0.0 1.7450401559906554 3.3776252592327387E-4 62-63 0.0 1.986838387783964E-5 0.0 2.00342848832196 3.3776252592327387E-4 64-65 0.0 1.986838387783964E-5 0.0 2.279678497759442 3.476967178621937E-4 66-67 0.0 1.986838387783964E-5 0.0 2.595416920154131 3.576309098011135E-4 68-69 0.0 1.986838387783964E-5 0.0 2.9453786337783985 3.675651017400333E-4 70-71 0.0 1.986838387783964E-5 0.0 3.332146528536364 3.774992936789531E-4 72-73 0.0 1.986838387783964E-5 0.0 3.7455479918825727 4.569728291903117E-4 74-75 0.0 1.986838387783964E-5 0.0 4.150316642433861 4.569728291903117E-4 76-77 0.0 1.986838387783964E-5 0.0 4.611958541835465 4.569728291903117E-4 78-79 0.0 1.986838387783964E-5 0.0 5.113257735457237 4.569728291903117E-4 80-81 0.0 1.986838387783964E-5 0.0 5.657204415072792 4.768412130681514E-4 82-83 0.0 1.986838387783964E-5 0.0 6.227059467265049 5.165779808238306E-4 84-85 0.0 1.986838387783964E-5 0.0 6.781278101345448 5.165779808238306E-4 86-87 0.0 1.986838387783964E-5 0.0 7.370991603223606 5.364463647016702E-4 88 0.0 1.986838387783964E-5 0.0 7.841117302541047 5.761831324573495E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5000 0.0 37.660297 94 GTACTAG 5195 0.0 37.115257 1 TAGGCAT 6770 0.0 31.850603 5 GCATCGT 5590 0.0 31.498646 8 CAACGCA 8865 0.0 29.940794 5 GTATCAA 10365 0.0 29.563698 1 CTAGGCA 7435 0.0 29.380936 4 CTGTGCG 7405 0.0 27.648582 9 TCTAACG 2895 0.0 27.261787 2 GTACATG 19590 0.0 27.000584 1 ATCAACG 9850 0.0 26.99441 3 TAGTACT 7800 0.0 26.861725 4 CTAACGC 2870 0.0 26.68083 3 AGGGCTA 5860 0.0 26.21463 5 TACATGG 19575 0.0 26.134834 2 ACATGGG 19205 0.0 25.953428 3 TAACGCC 2920 0.0 25.902203 4 ACTGTGC 8225 0.0 25.803284 8 CGCCTAC 2905 0.0 25.698912 7 CAGTACG 850 0.0 25.412676 9 >>END_MODULE