##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5033122
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.795023844047492	31.0	31.0	34.0	30.0	34.0
2	31.742283020359928	31.0	31.0	34.0	30.0	34.0
3	31.939605278791177	31.0	31.0	34.0	30.0	34.0
4	35.68901091608747	37.0	35.0	37.0	33.0	37.0
5	35.60671785822001	37.0	35.0	37.0	33.0	37.0
6	35.64324548461174	37.0	35.0	37.0	33.0	37.0
7	35.55900910011719	37.0	35.0	37.0	33.0	37.0
8	35.54029049961436	37.0	35.0	37.0	33.0	37.0
9	37.11824171955299	39.0	37.0	39.0	33.0	39.0
10-11	37.06035985219512	39.0	37.0	39.0	33.0	39.0
12-13	37.002107836845596	39.0	37.0	39.0	33.0	39.0
14-15	38.20344847591615	40.0	37.0	41.0	33.0	41.0
16-17	38.203564606619906	40.0	37.0	41.0	33.0	41.0
18-19	38.23957426424394	40.0	37.0	41.0	33.0	41.0
20-21	38.2509202041993	40.0	37.0	41.0	33.0	41.0
22-23	38.21689132510597	40.0	37.0	41.0	33.0	41.0
24-25	38.15617543147176	40.0	37.0	41.0	33.0	41.0
26-27	38.01857425669395	40.0	37.0	41.0	33.0	41.0
28-29	37.95224306106627	40.0	37.0	41.0	33.0	41.0
30-31	37.83375407947592	40.0	37.0	41.0	32.5	41.0
32-33	37.733484008533864	40.0	36.5	41.0	32.0	41.0
34-35	37.64924583588477	40.0	36.0	41.0	32.0	41.0
36-37	37.57152777143093	40.0	36.0	41.0	32.0	41.0
38-39	37.4605551981454	40.0	36.0	41.0	31.5	41.0
40-41	37.34710076568778	40.0	36.0	41.0	31.0	41.0
42-43	37.23230660810526	39.0	36.0	41.0	31.0	41.0
44-45	37.05367185615608	39.0	35.0	41.0	31.0	41.0
46-47	36.84938165615695	39.0	35.0	41.0	30.5	41.0
48-49	36.625346454943866	39.0	35.0	41.0	30.0	41.0
50-51	36.43073225723517	39.0	35.0	41.0	30.0	41.0
52-53	36.23909513816673	38.0	35.0	40.5	29.5	41.0
54-55	36.045170870088185	38.0	35.0	40.0	29.0	41.0
56-57	35.788915706791926	38.0	34.0	40.0	29.0	41.0
58-59	35.510813069899754	37.0	34.0	40.0	28.0	41.0
60-61	35.25653858579228	37.0	34.0	40.0	28.0	41.0
62-63	34.98265013246252	36.0	34.0	39.5	28.0	41.0
64-65	34.7118867772329	36.0	33.5	39.0	28.0	41.0
66-67	34.48872240728518	35.0	33.0	39.0	28.0	41.0
68-69	34.29887850920363	35.0	33.0	39.0	27.0	41.0
70-71	34.09272952255081	35.0	33.0	38.0	27.0	40.5
72-73	33.88634807580662	35.0	33.0	37.0	27.0	40.0
74-75	33.70886787564458	35.0	33.0	37.0	27.0	39.5
76-77	33.54296100909137	35.0	33.0	36.0	27.0	39.0
78-79	33.41403506610807	35.0	33.0	36.0	27.0	39.0
80-81	33.26372488089898	35.0	33.0	36.0	27.0	38.0
82-83	33.062669949188596	35.0	33.0	35.5	27.0	37.0
84-85	32.91707701502169	35.0	33.0	35.0	27.0	37.0
86-87	32.778381986369496	35.0	33.0	35.0	27.0	36.5
88-89	32.66226618389143	35.0	33.0	35.0	27.0	36.0
90-91	32.567528861807844	35.0	33.0	35.0	27.0	36.0
92-93	32.48222415431218	35.0	33.0	35.0	27.0	36.0
94-95	32.443027111204536	35.0	33.0	35.0	27.0	35.0
96-97	32.38931462420342	35.0	33.0	35.0	27.0	35.0
98-99	32.34718778920916	35.0	33.0	35.0	26.0	35.0
100	31.835739328392993	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4735774723752364
1101	2	0.5924346897053496
1101	3	0.5117235826448585
1101	4	0.3496489812480448
1101	5	0.35381872469982056
1101	6	0.4055146474656155
1101	7	0.39263778939486826
1101	8	0.3981980908216798
1101	9	0.49384680616484644
1101	10-11	0.5141798331065743
1101	12-13	0.48919128711392545
1101	14-15	0.6621001668309248
1101	16-17	0.6909270977060658
1101	18-19	0.6506835444756831
1101	20-21	0.6518105566552066
1101	22-23	0.6937146534384979
1101	24-25	0.6788604453087075
1101	26-27	0.7056084416473283
1101	28-29	0.7131393352146347
1101	30-31	0.7439167325001819
1101	32-33	0.759214004517375
1101	34-35	0.7444150321554801
1101	36-37	0.7568526420996236
1101	38-39	0.7853950868453907
1101	40-41	0.8436596063970754
1101	42-43	0.8376518356227223
1101	44-45	0.8240448583669959
1101	46-47	0.7587808565790013
1101	48-49	0.8222013400657531
1101	50-51	0.7962042914767764
1101	52-53	0.7542348398475767
1101	54-55	0.7231113512593907
1101	56-57	0.7283819837523922
1101	58-59	0.7439774442316676
1101	60-61	0.8176989285390022
1101	62-63	0.7500899140161152
1101	64-65	0.7028387569367567
1101	66-67	0.6664589776563616
1101	68-69	0.6317049639598835
1101	70-71	0.6545567409185082
1101	72-73	0.5886749346950353
1101	74-75	0.54455463550714
1101	76-77	0.5625910774613914
1101	78-79	0.5487641874514395
1101	80-81	0.5757259578503877
1101	82-83	0.5583426567867633
1101	84-85	0.540853687406532
1101	86-87	0.5519611171271848
1101	88-89	0.5253091479809484
1101	90-91	0.5638326185619675
1101	92-93	0.5887714649708258
1101	94-95	0.581724005035376
1101	96-97	0.6565457601022757
1101	98-99	0.6178304276458562
1101	100	0.653148819410184
1102	1	0.4341621027542253
1102	2	0.5359873045107051
1102	3	0.4309136553315618
1102	4	0.30168481906319045
1102	5	0.3082244564378769
1102	6	0.3335068150227656
1102	7	0.36434707132958266
1102	8	0.36547818878305094
1102	9	0.42465635413910974
1102	10-11	0.4854685095264202
1102	12-13	0.49098090584400467
1102	14-15	0.6491693071407383
1102	16-17	0.6369683702741966
1102	18-19	0.6125584418270762
1102	20-21	0.5791410848030694
1102	22-23	0.5845364038413123
1102	24-25	0.6001341378365694
1102	26-27	0.6146993137028929
1102	28-29	0.6184938112496496
1102	30-31	0.6714417654038343
1102	32-33	0.6726098069759772
1102	34-35	0.677088083151574
1102	36-37	0.6978421738916012
1102	38-39	0.6859886709613647
1102	40-41	0.6375173233231664
1102	42-43	0.7084173367247857
1102	44-45	0.6701477454790137
1102	46-47	0.7267850579992583
1102	48-49	0.7170368447677973
1102	50-51	0.6693330681041019
1102	52-53	0.6154265382424811
1102	54-55	0.6856153303121744
1102	56-57	0.7253500897809744
1102	58-59	0.7027151676717125
1102	60-61	0.6835160253601131
1102	62-63	0.629332827829316
1102	64-65	0.666456236313266
1102	66-67	0.6205721775542514
1102	68-69	0.6227949444618588
1102	70-71	0.6233130220516969
1102	72-73	0.5987331960955373
1102	74-75	0.5661418984990476
1102	76-77	0.5436161915222328
1102	78-79	0.5295573570620391
1102	80-81	0.483377017867916
1102	82-83	0.5044454316999136
1102	84-85	0.4789930139333407
1102	86-87	0.5402997609399023
1102	88-89	0.5209170297058208
1102	90-91	0.5899654584980993
1102	92-93	0.548306561739146
1102	94-95	0.5200962443955319
1102	96-97	0.47168563923523976
1102	98-99	0.4853157346695198
1102	100	0.5379257755360456
1103	1	0.28164651566299526
1103	2	-0.17887557239811258
1103	3	0.19207062625068616
1103	4	0.17509261952491784
1103	5	0.22825115501476745
1103	6	0.2892609768324519
1103	7	0.35369992065289324
1103	8	0.31583003830536427
1103	9	0.3857893226759401
1103	10-11	0.4298487113753495
1103	12-13	0.45550178019047394
1103	14-15	0.5186670311147026
1103	16-17	0.5404087442337726
1103	18-19	0.5389358619799225
1103	20-21	0.46629712717243876
1103	22-23	0.48441895083063
1103	24-25	0.4345798361928388
1103	26-27	0.5089055016190827
1103	28-29	0.550138446818039
1103	30-31	0.6068903485200536
1103	32-33	0.6280400995610336
1103	34-35	0.640988363964226
1103	36-37	0.609057639599321
1103	38-39	0.6140049161348244
1103	40-41	0.6369872662624871
1103	42-43	0.6021375605388499
1103	44-45	0.6031295936707295
1103	46-47	0.646595899407366
1103	48-49	0.6193940865190584
1103	50-51	0.6468278965135852
1103	52-53	0.6567562579342692
1103	54-55	0.5740252607649694
1103	56-57	0.5812143350739873
1103	58-59	0.49309894549095645
1103	60-61	0.6031339609092257
1103	62-63	0.5920385921312459
1103	64-65	0.5668357522806247
1103	66-67	0.5874326097245444
1103	68-69	0.5383058710014978
1103	70-71	0.4871200966403535
1103	72-73	0.5097292053455647
1103	74-75	0.5009813403643335
1103	76-77	0.4906508188584695
1103	78-79	0.47748936258141583
1103	80-81	0.5042882119782846
1103	82-83	0.4878163630226595
1103	84-85	0.451758216451573
1103	86-87	0.4918552687661091
1103	88-89	0.4744259030080542
1103	90-91	0.47176625979997056
1103	92-93	0.4594179218548575
1103	94-95	0.4877232049044906
1103	96-97	0.4760319575519958
1103	98-99	0.4853021121289416
1103	100	0.5322425317377508
1104	1	0.42081302127411035
1104	2	0.4548236428438983
1104	3	0.3888073587686698
1104	4	0.3135186090978266
1104	5	0.2976920595166064
1104	6	0.32476076612647375
1104	7	0.3482060313478925
1104	8	0.3432840478860584
1104	9	0.3910291952610123
1104	10-11	0.38488644936913374
1104	12-13	0.4276316244030198
1104	14-15	0.5684165750077455
1104	16-17	0.5388324160478888
1104	18-19	0.5171018701306593
1104	20-21	0.5611110192194957
1104	22-23	0.5123348285191014
1104	24-25	0.5096656529238004
1104	26-27	0.5889147127910945
1104	28-29	0.5686290930760123
1104	30-31	0.5708974985135669
1104	32-33	0.5795565721732601
1104	34-35	0.5680574906146632
1104	36-37	0.5911232152675652
1104	38-39	0.5567933210385618
1104	40-41	0.5363256615555443
1104	42-43	0.48804981902602407
1104	44-45	0.5506884300804273
1104	46-47	0.5294816438064132
1104	48-49	0.5419543436403416
1104	50-51	0.4639301628468573
1104	52-53	0.5121165934209202
1104	54-55	0.5376833820021005
1104	56-57	0.4781980074290857
1104	58-59	0.5000290595479413
1104	60-61	0.540089009234137
1104	62-63	0.5890474971002391
1104	64-65	0.5178668618667004
1104	66-67	0.4673197623359613
1104	68-69	0.4724149371147277
1104	70-71	0.45893845823708546
1104	72-73	0.48566654969866363
1104	74-75	0.430859221801974
1104	76-77	0.4005338388308388
1104	78-79	0.39366008969793853
1104	80-81	0.36830069838764956
1104	82-83	0.43557432077091107
1104	84-85	0.4404349198757629
1104	86-87	0.4043788237901751
1104	88-89	0.407777380986694
1104	90-91	0.43068460863282354
1104	92-93	0.4018434754241653
1104	94-95	0.3651459754588018
1104	96-97	0.39627744903236106
1104	98-99	0.4148177727357165
1104	100	0.4572166959883077
1105	1	0.1382564693765218
1105	2	-0.45499014016428774
1105	3	-0.01222119277214162
1105	4	0.10988811418298639
1105	5	0.0908042768006112
1105	6	0.19915000079517142
1105	7	0.25186150659607875
1105	8	0.2607051422605835
1105	9	0.3258731704412412
1105	10-11	0.28955974484115643
1105	12-13	0.2574305185045276
1105	14-15	0.3050747453058875
1105	16-17	0.4150547370259474
1105	18-19	0.4453850813395661
1105	20-21	0.41717066378589607
1105	22-23	0.383750249340963
1105	24-25	0.4167862105924556
1105	26-27	0.4049875353638086
1105	28-29	0.47018260502785836
1105	30-31	0.4586737859013752
1105	32-33	0.4751688213881309
1105	34-35	0.5177726142482229
1105	36-37	0.4604024081095943
1105	38-39	0.5218490935471962
1105	40-41	0.5194866382267449
1105	42-43	0.5448111449042088
1105	44-45	0.5324379988227861
1105	46-47	0.520059895453798
1105	48-49	0.500611403974915
1105	50-51	0.48635237766591644
1105	52-53	0.5209569454132037
1105	54-55	0.5497951307828615
1105	56-57	0.5708954544322324
1105	58-59	0.4764303885678771
1105	60-61	0.4502171786696252
1105	62-63	0.5271497148251214
1105	64-65	0.48556871660235146
1105	66-67	0.5738148441975852
1105	68-69	0.565731949001794
1105	70-71	0.5128397756940117
1105	72-73	0.5038207215946642
1105	74-75	0.47228702755656826
1105	76-77	0.43345214304173396
1105	78-79	0.3534723048051305
1105	80-81	0.36309320907408704
1105	82-83	0.3282918301175286
1105	84-85	0.4112805328017899
1105	86-87	0.40021336275238895
1105	88-89	0.3680833470006277
1105	90-91	0.3828161244230657
1105	92-93	0.2971378799186084
1105	94-95	0.32418410575022705
1105	96-97	0.2719390597593332
1105	98-99	0.2926916938914701
1105	100	0.4523374474911037
1106	1	0.28917283221466406
1106	2	0.32338175309573103
1106	3	0.2830954240829975
1106	4	0.1752084307516384
1106	5	0.2264397873949946
1106	6	0.22432223456308265
1106	7	0.1899182622436797
1106	8	0.23132990254863728
1106	9	0.2555692876415421
1106	10-11	0.2906340113949142
1106	12-13	0.26668910691350334
1106	14-15	0.33097171160507344
1106	16-17	0.3438795970441717
1106	18-19	0.35530834709617665
1106	20-21	0.3442403914245631
1106	22-23	0.32701780261066205
1106	24-25	0.3496032121649577
1106	26-27	0.36175170208528584
1106	28-29	0.3436599651162382
1106	30-31	0.31420818527491434
1106	32-33	0.34429407300756765
1106	34-35	0.3614113456856529
1106	36-37	0.4050306004692672
1106	38-39	0.3705154078805961
1106	40-41	0.36831702246495013
1106	42-43	0.4046892693001283
1106	44-45	0.41647596655251107
1106	46-47	0.3677430685840406
1106	48-49	0.4044511081920703
1106	50-51	0.4068918074644401
1106	52-53	0.43545984380775593
1106	54-55	0.3843014468243737
1106	56-57	0.41933693672555705
1106	58-59	0.3229531684416287
1106	60-61	0.34601332295545006
1106	62-63	0.32836465761167233
1106	64-65	0.3946085213351722
1106	66-67	0.3502580734469234
1106	68-69	0.33487141820394584
1106	70-71	0.42308731801952604
1106	72-73	0.4062285916943793
1106	74-75	0.376477765390554
1106	76-77	0.3562298459588078
1106	78-79	0.3049857489898997
1106	80-81	0.3058154384253342
1106	82-83	0.29456833803642724
1106	84-85	0.2980581023263724
1106	86-87	0.32412988618994376
1106	88-89	0.3109580136890031
1106	90-91	0.2743122318688549
1106	92-93	0.24519886513639477
1106	94-95	0.2824169323420662
1106	96-97	0.26392662712235904
1106	98-99	0.2927870895155209
1106	100	0.28080051965865493
1107	1	0.014790816721671263
1107	2	-0.5460556046063196
1107	3	-0.08413369608080856
1107	4	0.042503905992070656
1107	5	0.04058965748131982
1107	6	0.1449071683860268
1107	7	0.14897319311806
1107	8	0.1645918895466849
1107	9	0.21004492938723018
1107	10-11	0.2329095861530348
1107	12-13	0.18012836579474367
1107	14-15	0.21418907930906528
1107	16-17	0.26163842430542417
1107	18-19	0.2948260820663151
1107	20-21	0.2015099606822588
1107	22-23	0.22194699413265084
1107	24-25	0.18880382572732657
1107	26-27	0.28473389693667883
1107	28-29	0.14479682080947498
1107	30-31	0.24520149952302006
1107	32-33	0.25839648468094367
1107	34-35	0.23203940639973553
1107	36-37	0.20188133892800408
1107	38-39	0.24034629802006435
1107	40-41	0.23086146142698993
1107	42-43	0.18115157365409829
1107	44-45	0.22432899073292845
1107	46-47	0.23979651622311593
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2216	92-93	-0.8069208886870776
2216	94-95	-0.7121852694624664
2216	96-97	-0.7988685249311231
2216	98-99	-0.7566417172378976
2216	100	-0.7903013024750152
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	10.0
13	58.0
14	261.0
15	743.0
16	1843.0
17	3879.0
18	6809.0
19	10522.0
20	15304.0
21	21332.0
22	29332.0
23	34093.0
24	37796.0
25	41528.0
26	49902.0
27	62981.0
28	80277.0
29	100936.0
30	124860.0
31	153405.0
32	190984.0
33	239594.0
34	324057.0
35	441208.0
36	652645.0
37	1092364.0
38	955856.0
39	359730.0
40	810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8392720565749	21.76529698385229	13.442480336231904	24.952950623340904
2	14.942916982999774	20.437466582607	40.0216136396014	24.598002794791824
3	17.922491758955175	26.589422414569007	29.71659865937856	25.771487167097256
4	12.24960480914577	17.286444904015426	39.24609867032239	31.217851616516413
5	12.53768151303047	38.78443988959208	35.47160604112242	13.206272556255033
6	29.474191168026525	39.2070567731122	17.92233528215688	13.3964167767044
7	25.934725435113766	32.23631755243131	24.218013160454106	17.610943852000823
8	25.901756404871566	35.376571440151864	19.889404627982394	18.83226752699418
9	25.995932544452526	14.49734379575937	21.72613737556928	37.78058628421882
10-11	25.488633098899648	26.020509735309417	27.66845309928907	20.822404066501864
12-13	25.752296487150524	23.75769949546226	28.051257251463408	22.438746765923813
14-15	23.043741582540086	24.802719974629888	25.64001506946379	26.513523373366233
16-17	21.769086008125836	27.237964613471533	27.022266516893982	23.97068286150865
18-19	21.174759920383412	27.639326048524154	29.200732666523876	21.98518136456855
20-21	22.72157681946324	26.779974802900547	28.358707592647818	22.139740784988394
22-23	23.087061371374926	26.403256450439216	28.074894386398014	22.434787791787844
24-25	22.603266918624264	26.41372492063574	28.951195699210153	22.031812461529842
26-27	22.58221476626744	26.659045141739803	27.85815380479697	22.900586287195786
28-29	21.77165584303341	27.263455962323185	28.40281836998984	22.562069824653566
30-31	22.70079088088864	26.81611929931363	28.269938618614848	22.213151201182885
32-33	23.1014169734014	27.04713893285321	27.447685551830453	22.40375854191494
34-35	23.001166875517452	26.799383441493994	28.313867857556545	21.885581825432006
36-37	22.730434509634378	26.407973023503107	27.970522073575804	22.89107039328671
38-39	22.866503136621763	26.383962081586738	28.68066778432949	22.068866997462013
40-41	22.380440295026773	25.695830752503053	29.145402860362967	22.778326092107203
42-43	22.566306493303166	25.91269311836646	28.27314378029143	23.247856608038944
44-45	22.699211344370354	26.802519390549246	28.06872156089203	22.429547704188373
46-47	22.781347243321342	26.50229817596315	27.416631267829388	23.299723312886115
48-49	23.61111800989252	26.243295626249854	27.507954846868575	22.63763151698905
50-51	22.989975208230597	26.271377884342957	27.249339475577983	23.489307431848463
52-53	22.315483312345698	26.848226607660212	27.15442820579354	23.681861874200546
54-55	22.45407058031596	26.788980873230688	26.962184163526953	23.7947643829264
56-57	23.556731985692764	26.189215729614894	26.79278796888807	23.46126431580427
58-59	22.29625306408311	26.630608793266592	27.790596772871655	23.28254136977864
60-61	22.498320126808075	27.329112887875866	26.931883973314356	23.240683012001707
62-63	23.016946539344765	26.479608481574658	26.458279771481795	24.04516520759878
64-65	22.108275936883707	26.690501442245985	26.983152802574622	24.21806981829568
66-67	22.803579964880644	26.25138035596991	26.9912889057726	23.953750773376843
68-69	22.235681030547568	26.22866540732488	27.357254866703656	24.1783986954239
70-71	22.24199364265827	26.002068117952064	27.385148075874437	24.37079016351523
72-73	22.772028971282634	25.98913755716631	27.78275591173828	23.456077559812776
74-75	22.91961485065156	26.42560642147379	27.238721566426342	23.416057161448308
76-77	22.599145078718802	26.429749941287245	26.78567241899329	24.185432561000663
78-79	22.332404050827975	26.46022243373336	26.98314128614116	24.224232229297503
80-81	21.962535164449466	26.899759475513363	27.534038372452628	23.603666987584543
82-83	22.86955573221104	25.8138200441365	28.482216353869234	22.83440786978323
84-85	23.17792848843765	26.404199631518892	26.97756576950608	23.440306110537374
86-87	21.75287570723457	27.06555115292063	27.45154532287073	23.73002781697407
88-89	22.654585736355553	25.88972177100875	27.291675980706277	24.16401651192942
90-91	22.316799224927344	25.881044587640105	27.48593059733825	24.316225590094298
92-93	22.672665870270002	26.251801231474044	27.097803789163123	23.97772910909283
94-95	21.513126320462806	26.16574507258193	27.783724596473718	24.537404010481545
96-97	21.87288733104485	26.15493728592656	27.29197975354675	24.680195629481837
98-99	21.191466127931786	26.891595632528027	27.20783679639247	24.70910144314772
100	22.073643703732575	26.07186121223739	26.413923000503488	25.440572083526547
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	87.0
1	58.5
2	56.0
3	83.5
4	63.0
5	55.0
6	100.5
7	224.0
8	419.5
9	494.0
10	399.5
11	307.0
12	309.0
13	379.5
14	520.5
15	755.0
16	1013.5
17	1370.0
18	1875.5
19	2443.0
20	3181.0
21	4112.0
22	5118.5
23	6248.5
24	7364.5
25	8765.5
26	10959.0
27	13361.5
28	17700.5
29	27424.5
30	40948.0
31	53813.5
32	70998.5
33	87462.0
34	99584.0
35	114810.0
36	128491.0
37	152602.0
38	165106.0
39	155083.5
40	144578.0
41	133660.0
42	131279.5
43	140312.0
44	155258.5
45	184359.0
46	213529.0
47	234175.0
48	248343.5
49	243281.5
50	229215.0
51	230975.5
52	232107.5
53	220798.5
54	209504.0
55	179767.0
56	154689.5
57	127285.0
58	98113.0
59	94432.5
60	77883.5
61	51549.5
62	36943.0
63	30180.5
64	21733.5
65	9767.0
66	4734.0
67	3076.0
68	2191.5
69	1508.5
70	1092.0
71	785.5
72	563.0
73	430.5
74	309.5
75	222.5
76	157.0
77	96.5
78	59.5
79	31.5
80	18.5
81	10.5
82	4.0
83	2.0
84	2.0
85	1.0
86	0.5
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012775370833450886
2	0.0037551245529116916
3	0.00164907586186069
4	0.027338896215907346
5	0.0029802575816759457
6	0.0
7	2.582889904119153E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008503668299715366
16-17	0.0032087439962711017
18-19	0.0
20-21	5.9605151633518915E-5
22-23	6.854592437854675E-4
24-25	0.0
26-27	1.6888126296163693E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.569728291903117E-4
36-37	0.0
38-39	0.0
40-41	3.8743348561787297E-4
42-43	7.251960115411468E-4
44-45	0.0
46-47	0.0
48-49	5.364463647016702E-4
50-51	0.0
52-53	0.0
54-55	3.774992936789531E-4
56-57	8.940772745027838E-5
58-59	1.8874964683947656E-4
60-61	7.947353551135855E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001380852679509855
70-71	8.642746986860243E-4
72-73	0.0
74-75	1.8874964683947656E-4
76-77	0.002861047278408908
78-79	5.463805566405901E-4
80-81	0.0032286123801489415
82-83	0.0019669700039061243
84-85	0.005950580971412971
86-87	0.008016892894708295
88-89	0.007887748399502336
90-91	0.006030054506924331
92-93	0.009020246280539197
94-95	0.0016987468215552892
96-97	0.004778346322620433
98-99	0.0019073648522726053
100	0.08356642259019352
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5033122.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.95525234578301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.42635291970142	15.018784413360029
2	16.336631238552382	7.500229851219431
3	6.818902347502936	4.695888723245461
4	3.491066112217108	3.2055321424702132
5	2.1153750727408056	2.427948430037214
6	1.2677962057190275	1.7461549095183901
7	0.8564098625476132	1.376137315433846
8	0.6269372706910576	1.1513202602951758
9	0.41922077948684	0.866098690354464
>10	2.030086327573049	8.373792022022258
>50	0.21495718419930307	3.5114603093364503
>100	0.2843998411813021	14.623590768026999
>500	0.059736169227634445	9.605320885956546
>1k	0.04875716272629831	19.939393411871485
>5k	0.0027663638426268546	3.9082464469442595
>10k+	6.051420905746244E-4	2.0501014199078798
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	21261	0.4224217096267486	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	15085	0.2997145707972109	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14517	0.28842932875459804	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	13995	0.27805803237036575	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	13782	0.2738260666043859	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13480	0.2678258146732783	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	11188	0.2222874788252699	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8502	0.16892099972939262	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7551	0.15002616666156712	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7435	0.14772143413173772	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7251	0.1440656514982152	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7225	0.14354907351739138	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6693	0.1329790932943807	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6673	0.1325817256168239	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6626	0.13164791157456546	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6613	0.13138962258415354	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6585	0.130833307835574	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6501	0.1291643635898355	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6493	0.12900541651881278	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6412	0.12739607742470777	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6362	0.12640265823081578	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6347	0.1261046324726482	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6232	0.12381976832669664	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6206	0.12330319034587281	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5859	0.11640886114026244	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5831	0.11585254639168294	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5811	0.11545517871412614	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5707	0.11338886679083084	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5689	0.1130312358810297	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5664	0.11253452628408372	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5411	0.10750782516299029	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5363	0.10655414273685399	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5293	0.1051633558654052	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5256	0.10442822566192514	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5117	0.10166652030290545	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5092	0.10116981070595944	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5087	0.10107046878657024	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5047	0.10027573343145667	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.986838387783964E-5
7	0.0	0.0	0.0	0.0	1.986838387783964E-5
8	0.0	0.0	0.0	0.0	1.986838387783964E-5
9	0.0	0.0	0.0	0.0	1.986838387783964E-5
10-11	0.0	0.0	0.0	0.0	1.986838387783964E-5
12-13	0.0	0.0	0.0	0.0	3.973676775567928E-5
14-15	0.0	0.0	0.0	0.0	3.973676775567928E-5
16-17	0.0	0.0	0.0	0.0	5.9605151633518915E-5
18-19	0.0	0.0	0.0	0.0	5.9605151633518915E-5
20-21	0.0	0.0	0.0	0.0	9.934191938919819E-5
22-23	0.0	0.0	0.0	7.947353551135855E-5	1.09276111328118E-4
24-25	0.0	0.0	0.0	2.18552222656236E-4	1.3907868714487748E-4
26-27	0.0	0.0	0.0	5.761831324573495E-4	1.3907868714487748E-4
28-29	0.0	0.0	0.0	0.0017583519731888082	1.3907868714487748E-4
30-31	0.0	0.0	0.0	0.007679130368785021	1.3907868714487748E-4
32-33	0.0	0.0	0.0	0.02263008923685935	1.589470710227171E-4
34-35	0.0	0.0	0.0	0.04513103397851274	2.1855222265623604E-4
36-37	0.0	0.0	0.0	0.08395385607581139	2.1855222265623604E-4
38-39	0.0	0.0	0.0	0.14678762008947926	2.1855222265623604E-4
40-41	0.0	0.0	0.0	0.2170223570976424	2.1855222265623604E-4
42-43	0.0	0.0	0.0	0.29357524017895853	2.2848641459515586E-4
44-45	0.0	0.0	0.0	0.38528770015906627	2.582889904119153E-4
46-47	0.0	0.0	0.0	0.5028588617561824	2.7815737428975497E-4
48-49	0.0	0.0	0.0	0.6386294629853995	3.178941420454342E-4
50-51	0.0	0.0	0.0	0.785645569489474	3.3776252592327387E-4
52-53	0.0	0.0	0.0	0.9331285830146776	3.973676775567928E-4
54-55	0.0	0.0	0.0	1.0941121633848732	4.669070211292315E-4
56-57	0.0	0.0	0.0	1.2921900164549955	4.867754050070712E-4
58-59	0.0	0.0	0.0	1.5306900965245824	4.96709596945991E-4
60-61	0.0	0.0	0.0	1.782025152579254	5.066437888849108E-4
62-63	0.0	0.0	0.0	2.0451123576976675	6.357882840908684E-4
64-65	0.0	0.0	0.0	2.3273328164904408	6.357882840908684E-4
66-67	0.0	0.0	0.0	2.649081425008176	6.357882840908684E-4
68-69	0.0	0.0	0.0	3.005867928494481	6.357882840908684E-4
70-71	0.0	0.0	0.0	3.3997785072565296	6.457224760297883E-4
72-73	0.0	0.0	0.0	3.820660417132746	7.351302034800666E-4
74-75	0.0	0.0	0.0	4.234816879066313	7.351302034800666E-4
76-77	0.0	0.0	0.0	4.709144741573917	7.450643954189864E-4
78-79	0.0	0.0	0.0	5.225007460578146	7.549985873579062E-4
80-81	0.0	0.0	0.0	5.784709768608828	7.848011631746657E-4
82-83	0.0	0.0	0.0	6.370946303308364	8.146037389914252E-4
84-85	0.0	0.0	0.0	6.940662276813477	8.146037389914252E-4
86-87	0.0	0.0	0.0	7.5459625258438	8.24537930930345E-4
88	0.0	0.0	0.0	8.02925102948031	8.344721228692648E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5060	0.0	40.253407	94
GGTATCA	9330	0.0	38.196537	1
GTATCAA	14805	0.0	35.376366	1
CAACGCA	15755	0.0	31.86008	5
CTGTGCG	7090	0.0	31.293839	9
CAGTACG	940	0.0	31.004658	9
GTACTAG	5070	0.0	30.972406	1
ATCAACG	16285	0.0	30.830536	3
GTGGTAT	2225	0.0	30.00508	1
TAGTACT	7635	0.0	29.000767	4
ACTGTGC	8090	0.0	28.4653	8
TAGGCAT	7025	0.0	28.099398	5
TATCAAC	18670	0.0	28.050362	2
TATTCTC	2980	0.0	27.758144	5
AACGCAG	18825	0.0	27.686563	6
GTACTGT	8130	0.0	27.400612	6
TACTGTG	9215	0.0	26.775429	7
CTAGTAC	8580	0.0	26.628492	3
ACGCAGA	19135	0.0	26.108221	7
CTAGGCA	7770	0.0	25.895266	4
>>END_MODULE