##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146446_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4868193 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.068113774453888 31.0 30.0 34.0 26.0 34.0 2 30.21949787118136 31.0 30.0 34.0 26.0 34.0 3 30.38912960106553 31.0 30.0 34.0 26.0 34.0 4 33.84620926080786 35.0 35.0 37.0 28.0 37.0 5 34.09059665465194 37.0 35.0 37.0 29.0 37.0 6 34.22694313886076 37.0 35.0 37.0 30.0 37.0 7 34.10348377724548 37.0 35.0 37.0 29.0 37.0 8 34.053574909622526 37.0 35.0 37.0 29.0 37.0 9 35.38554469800191 39.0 35.0 39.0 28.0 39.0 10-11 35.31023307826949 38.5 35.0 39.0 28.0 39.0 12-13 35.298442666508905 38.0 35.0 39.0 28.0 39.0 14-15 36.251566135525024 39.0 35.5 41.0 27.0 41.0 16-17 36.06090750715923 39.0 35.0 41.0 27.0 41.0 18-19 35.99250461105383 39.0 35.0 41.0 27.0 41.0 20-21 36.330518531208604 39.0 35.0 41.0 27.0 41.0 22-23 36.02186519720972 39.0 34.5 41.0 27.0 41.0 24-25 36.11005212817159 39.0 35.0 41.0 27.0 41.0 26-27 36.16990164523058 39.0 35.0 41.0 27.0 41.0 28-29 35.99506449312918 39.0 35.0 41.0 27.0 41.0 30-31 35.92460539259639 39.0 34.5 41.0 27.0 41.0 32-33 36.02949544112158 39.0 35.0 41.0 27.0 41.0 34-35 36.067404270948174 39.0 35.0 41.0 27.5 41.0 36-37 35.981583926520585 39.0 35.0 41.0 27.0 41.0 38-39 35.81406827954439 39.0 34.0 41.0 27.0 41.0 40-41 35.77029248840381 39.0 34.0 41.0 27.0 41.0 42-43 35.595471461382076 38.0 34.0 40.0 26.5 41.0 44-45 35.3939564433867 38.0 33.5 40.0 26.0 41.0 46-47 35.25655340698284 38.0 33.0 40.0 26.0 41.0 48-49 35.118750735642564 38.0 33.0 40.0 26.0 41.0 50-51 34.894300000020536 38.0 33.0 40.0 25.5 41.0 52-53 34.69958956023313 37.5 33.0 40.0 25.0 41.0 54-55 34.487235099347956 37.0 33.0 40.0 25.0 41.0 56-57 34.32019724772621 36.5 32.5 40.0 25.0 41.0 58-59 34.04377065576488 36.0 32.0 39.5 24.5 41.0 60-61 33.78277227299739 35.5 32.0 39.0 24.5 41.0 62-63 33.51126608990235 35.0 32.0 39.0 24.0 41.0 64-65 33.219338366412344 35.0 31.0 38.5 23.5 40.0 66-67 32.92316204390417 35.0 31.0 38.0 23.0 40.0 68-69 32.630942014829735 35.0 31.0 37.0 23.0 40.0 70-71 32.574959949204974 35.0 31.0 37.0 23.0 40.0 72-73 32.37846989632499 35.0 31.0 36.5 23.0 39.5 74-75 32.13183649456791 35.0 31.0 36.0 23.0 39.0 76-77 31.918381214549218 35.0 30.0 36.0 23.0 39.0 78-79 31.708276664462563 34.0 30.0 35.0 22.0 38.5 80-81 31.497390510195466 34.0 30.0 35.0 21.5 37.0 82-83 31.279901084447555 34.0 30.0 35.0 21.0 37.0 84-85 31.116814904421418 34.0 30.0 35.0 21.0 36.0 86-87 30.98656298137728 34.0 30.0 35.0 20.0 36.0 88-89 31.0212845916339 34.0 30.0 35.0 20.5 36.0 90-91 30.95645283578527 34.0 30.0 35.0 20.0 36.0 92-93 30.818890397319908 34.0 30.0 35.0 20.0 35.0 94-95 30.812733389986796 34.0 30.0 35.0 20.0 35.0 96-97 30.738253187579048 34.0 30.0 35.0 20.0 35.0 98-99 30.673872317716246 34.0 30.0 35.0 20.0 35.0 100 30.148099510434363 34.0 29.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9016200447533933 1101 2 1.0566968927159515 1101 3 1.0084560943795928 1101 4 1.1260552593501956 1101 5 1.0153633354556177 1101 6 0.6640980664921727 1101 7 0.8080230735985978 1101 8 0.8830502476908748 1101 9 1.0526273563817483 1101 10-11 1.064497160726411 1101 12-13 0.988190233836697 1101 14-15 1.2599032594052346 1101 16-17 1.3467109278553409 1101 18-19 1.490152346778146 1101 20-21 1.1242146522675256 1101 22-23 1.4590996635213145 1101 24-25 1.3250293904452377 1101 26-27 1.2404614250052433 1101 28-29 1.4148611814661436 1101 30-31 1.4460243194548568 1101 32-33 1.2668454679801826 1101 34-35 1.2053050490975323 1101 36-37 1.2062920968572897 1101 38-39 1.2309752637512403 1101 40-41 1.1789467254995003 1101 42-43 1.198729293680799 1101 44-45 1.2405163407033086 1101 46-47 1.2150351038091785 1101 48-49 1.1946302332157117 1101 50-51 1.2049442557517196 1101 52-53 1.2238703412937468 1101 54-55 1.2102220926679976 1101 56-57 1.097953849495788 1101 58-59 1.0250716925770291 1101 60-61 1.0953880855261886 1101 62-63 1.0091823141572576 1101 64-65 1.0727764321561608 1101 66-67 1.1190964650454092 1101 68-69 1.1336317154308801 1101 70-71 1.0438995424493953 1101 72-73 0.9863226523942359 1101 74-75 0.9653825550984934 1101 76-77 0.9591441376007737 1101 78-79 0.9886177596328487 1101 80-81 1.0146252162229104 1101 82-83 0.9453600091999803 1101 84-85 0.9637482949831018 1101 86-87 0.9443127426961659 1101 88-89 0.9586701413241023 1101 90-91 0.9798004543199994 1101 92-93 1.053448345387828 1101 94-95 0.975478996480355 1101 96-97 0.9933306455549413 1101 98-99 1.0016511843477396 1101 100 1.1037418351107746 1102 1 0.8867468671160523 1102 2 0.9812244862586859 1102 3 1.0130302507221458 1102 4 1.119965791511376 1102 5 0.9596157887363859 1102 6 0.752937659471776 1102 7 0.8718899759470773 1102 8 0.8346476176487485 1102 9 1.0820016133583437 1102 10-11 1.049951565160562 1102 12-13 0.9984917644649087 1102 14-15 1.2147002575906924 1102 16-17 1.4046150022306847 1102 18-19 1.4629641102724946 1102 20-21 1.2169574970825252 1102 22-23 1.4449321625827594 1102 24-25 1.342792440282551 1102 26-27 1.2080284209485939 1102 28-29 1.3643012416380031 1102 30-31 1.4199773082339036 1102 32-33 1.313688954663121 1102 34-35 1.2088337258901731 1102 36-37 1.120965277362437 1102 38-39 1.1325961627834786 1102 40-41 1.0968443342677006 1102 42-43 1.1773722592045104 1102 44-45 1.2389080209268997 1102 46-47 1.233586345049325 1102 48-49 1.1404183333700502 1102 50-51 1.2359573355706885 1102 52-53 1.1582812866802215 1102 54-55 1.2384839328110786 1102 56-57 1.1504397841931535 1102 58-59 1.1377706794581073 1102 60-61 1.1759480188365785 1102 62-63 1.1110549938385645 1102 64-65 1.0804045776218913 1102 66-67 1.0705579399338205 1102 68-69 1.158486923633859 1102 70-71 1.0516447792783339 1102 72-73 0.9269516282212038 1102 74-75 0.9358163504098869 1102 76-77 0.9729381389251799 1102 78-79 0.9577464734462957 1102 80-81 1.0734727673742128 1102 82-83 1.0648896623514403 1102 84-85 0.9922286426659106 1102 86-87 0.9934947977685376 1102 88-89 0.9922918524678899 1102 90-91 0.9732794137222456 1102 92-93 1.011570601415439 1102 94-95 1.0066477853261446 1102 96-97 1.0357607035889806 1102 98-99 1.0320172797988718 1102 100 1.088677882956265 1103 1 0.6529892015885679 1103 2 0.8266571094660691 1103 3 0.7183941902709421 1103 4 0.8316447416535055 1103 5 0.7140882780099744 1103 6 0.5232249570539409 1103 7 0.6394244042797155 1103 8 0.7181683970838364 1103 9 0.7246958067212788 1103 10-11 0.8637723382077169 1103 12-13 0.7361756173506606 1103 14-15 0.9318066607016249 1103 16-17 1.1810563476372167 1103 18-19 1.2324880158055151 1103 20-21 0.9335479077205733 1103 22-23 1.1151964036955349 1103 24-25 1.1025810593697187 1103 26-27 0.943817118554179 1103 28-29 1.1523244205428966 1103 30-31 1.1473809368497427 1103 32-33 0.9406707796442291 1103 34-35 0.9909612690886149 1103 36-37 0.9247278070397371 1103 38-39 1.0085379180828085 1103 40-41 0.8594664370709353 1103 42-43 0.9439080396510278 1103 44-45 0.8955006074568672 1103 46-47 0.9249943667044391 1103 48-49 0.9510896525860417 1103 50-51 0.9506192205984192 1103 52-53 0.9898342812930636 1103 54-55 0.9567820526275312 1103 56-57 0.8920621345290485 1103 58-59 0.8488309310364528 1103 60-61 0.7861866549582501 1103 62-63 0.6990733817887858 1103 64-65 0.8116192776016646 1103 66-67 0.8623074834623097 1103 68-69 0.8298672575634001 1103 70-71 0.7658771086404954 1103 72-73 0.7326288762436093 1103 74-75 0.7035354633912476 1103 76-77 0.7528877607059243 1103 78-79 0.696851509804322 1103 80-81 0.7728307794474176 1103 82-83 0.651063632562348 1103 84-85 0.6626366789600517 1103 86-87 0.7024663261895476 1103 88-89 0.6970917901854783 1103 90-91 0.7203029000467289 1103 92-93 0.7602079075148573 1103 94-95 0.7114283928724277 1103 96-97 0.7732428373204918 1103 98-99 0.769241500470244 1103 100 0.8367449989080171 1104 1 0.5542005313150611 1104 2 0.7378956116171125 1104 3 0.7098891792523894 1104 4 0.7648019730262519 1104 5 0.6880822293935083 1104 6 0.45161542674343735 1104 7 0.5174485808378009 1104 8 0.5305721427596382 1104 9 0.6747028118239413 1104 10-11 0.6862081282779613 1104 12-13 0.6152574064012057 1104 14-15 0.8393676814662285 1104 16-17 0.9493213627850992 1104 18-19 0.9339242856989571 1104 20-21 0.7956851171404864 1104 22-23 0.9622116110887049 1104 24-25 0.8751542784935396 1104 26-27 0.7577953005562321 1104 28-29 0.9410286003243797 1104 30-31 1.0096252637699834 1104 32-33 0.7971823780927778 1104 34-35 0.6965224515499457 1104 36-37 0.653540729189352 1104 38-39 0.8615544048289436 1104 40-41 0.7853449454159716 1104 42-43 0.6525991335847081 1104 44-45 0.7707345278493989 1104 46-47 0.7333472669222871 1104 48-49 0.7509757274953444 1104 50-51 0.804181565485429 1104 52-53 0.8754843416528217 1104 54-55 0.7708890126447159 1104 56-57 0.7157674019448947 1104 58-59 0.7320462573583697 1104 60-61 0.738610222967786 1104 62-63 0.6913803438074311 1104 64-65 0.6503922970010549 1104 66-67 0.7856572419179386 1104 68-69 0.6946289444974596 1104 70-71 0.6307220799248512 1104 72-73 0.5456677556167904 1104 74-75 0.5718705427332509 1104 76-77 0.6240901918452408 1104 78-79 0.6223273564210139 1104 80-81 0.6767012830142249 1104 82-83 0.5927872354393955 1104 84-85 0.6170578863026641 1104 86-87 0.5719650821254483 1104 88-89 0.4529480296778239 1104 90-91 0.5759380047756473 1104 92-93 0.6028721166018975 1104 94-95 0.5306105629373619 1104 96-97 0.5283063283055078 1104 98-99 0.5864760676497021 1104 100 0.7003925189963347 1105 1 0.5358432873266921 1105 2 0.6874811857903822 1105 3 0.6484585420041888 1105 4 0.7592113604373552 1105 5 0.6732281195111796 1105 6 0.29261029695792473 1105 7 0.43449964142170927 1105 8 0.45918030602548043 1105 9 0.4719938266384034 1105 10-11 0.5857448964721499 1105 12-13 0.5195727323401869 1105 14-15 0.7180554781460344 1105 16-17 0.8680425068338167 1105 18-19 0.8525821673223959 1105 20-21 0.6788206683326408 1105 22-23 0.9652696388247506 1105 24-25 0.790211838222973 1105 26-27 0.5908099780990881 1105 28-29 0.9025431110379643 1105 30-31 0.8016335442322315 1105 32-33 0.7153952688789573 1105 34-35 0.639020777315551 1105 36-37 0.5590118198619507 1105 38-39 0.6903831663008546 1105 40-41 0.5516655783346636 1105 42-43 0.5882041821540227 1105 44-45 0.6444623226225943 1105 46-47 0.5808052199383553 1105 48-49 0.6835640347770351 1105 50-51 0.7549675330377639 1105 52-53 0.7312099657969711 1105 54-55 0.6336343893217133 1105 56-57 0.5833343805631515 1105 58-59 0.6656758199276638 1105 60-61 0.6350122579415896 1105 62-63 0.5798518321767148 1105 64-65 0.5427042255513896 1105 66-67 0.6049736073214476 1105 68-69 0.5462460347467513 1105 70-71 0.6007173288886491 1105 72-73 0.5760665310490154 1105 74-75 0.6305930843979866 1105 76-77 0.6129331212616052 1105 78-79 0.5470761816511462 1105 80-81 0.6455592029681227 1105 82-83 0.6046043527959633 1105 84-85 0.5275957311299173 1105 86-87 0.6084806722448803 1105 88-89 0.581802375048877 1105 90-91 0.645559558153554 1105 92-93 0.6993414559962758 1105 94-95 0.5979775362345094 1105 96-97 0.5934757616841146 1105 98-99 0.6116535308835545 1105 100 0.6671624818317632 1106 1 0.6409095161484011 1106 2 0.7100371923224493 1106 3 0.716162254742919 1106 4 0.9046695799629845 1106 5 0.7400721160190713 1106 6 0.4768076100115195 1106 7 0.45658818182766936 1106 8 0.5265400233371551 1106 9 0.6427406631783299 1106 10-11 0.6149320063422365 1106 12-13 0.5828040875114411 1106 14-15 0.8148232640925741 1106 16-17 0.9024179136901154 1106 18-19 0.8241579579489056 1106 20-21 0.7148931845431079 1106 22-23 0.9453586098973261 1106 24-25 0.8350458549826811 1106 26-27 0.6753855261245647 1106 28-29 0.9136481634116436 1106 30-31 0.8250689433293132 1106 32-33 0.7561829649608214 1106 34-35 0.7248111432342625 1106 36-37 0.6406014671553208 1106 38-39 0.7289013265955973 1106 40-41 0.669607465779535 1106 42-43 0.6622486531543288 1106 44-45 0.642422307188582 1106 46-47 0.6970941931862171 1106 48-49 0.6242008324393495 1106 50-51 0.6949516585253903 1106 52-53 0.6506437431964542 1106 54-55 0.662821737486496 1106 56-57 0.6606288764439867 1106 58-59 0.6480608030311217 1106 60-61 0.6492541792532407 1106 62-63 0.6406307944172411 1106 64-65 0.6754437035167911 1106 66-67 0.67037979315581 1106 68-69 0.6002031727803683 1106 70-71 0.6061900986889341 1106 72-73 0.5077601199393484 1106 74-75 0.5690871275665401 1106 76-77 0.6162240732576265 1106 78-79 0.646731912185178 1106 80-81 0.6616960778123939 1106 82-83 0.6246637201769687 1106 84-85 0.6169782280196223 1106 86-87 0.613110738354127 1106 88-89 0.6155445903225747 1106 90-91 0.5677415032752755 1106 92-93 0.619026447077502 1106 94-95 0.6024784636349452 1106 96-97 0.6530985009971495 1106 98-99 0.6724659054458648 1106 100 0.6097791425362402 1107 1 0.4560816345298626 1107 2 0.6910811646013464 1107 3 0.6502612194175903 1107 4 0.8095172060875626 1107 5 0.6994904362802075 1107 6 0.43309810636262114 1107 7 0.48740542421019484 1107 8 0.554617188295154 1107 9 0.6414127166500165 1107 10-11 0.6570053903609079 1107 12-13 0.5520563150321962 1107 14-15 0.7953095191721573 1107 16-17 0.8506211080376431 1107 18-19 0.9187135568755878 1107 20-21 0.7210595453986741 1107 22-23 0.8969989031464252 1107 24-25 0.651132219668078 1107 26-27 0.5611102984377254 1107 28-29 0.7873579396510948 1107 30-31 0.8615059330251569 1107 32-33 0.6959983126695519 1107 34-35 0.561068479149256 1107 36-37 0.5752685322200719 1107 38-39 0.662647923918037 1107 40-41 0.4533021488433491 1107 42-43 0.5926384878054449 1107 44-45 0.5245762705313268 1107 46-47 0.6075660080869341 1107 48-49 0.5984156911531926 1107 50-51 0.6869755626139309 1107 52-53 0.6256510216012856 1107 54-55 0.5695428896005978 1107 56-57 0.5351690367102648 1107 58-59 0.5783465345402163 1107 60-61 0.6294571300323142 1107 62-63 0.5580103638984468 1107 64-65 0.5746131652254007 1107 66-67 0.5583076496953367 1107 68-69 0.5842155933481123 1107 70-71 0.5060164985201965 1107 72-73 0.4581882667844184 1107 74-75 0.5089105784021015 1107 76-77 0.5246007552116296 1107 78-79 0.47040213397724884 1107 80-81 0.549367007593986 1107 82-83 0.5556884212538051 1107 84-85 0.6036916174060245 1107 86-87 0.6734104109708525 1107 88-89 0.6634986830237715 1107 90-91 0.6723273916222539 1107 92-93 0.7570912430700929 1107 94-95 0.7159514987173843 1107 96-97 0.7043969050168108 1107 98-99 0.6390398715504482 1107 100 0.6769995635550465 1108 1 0.436629813619124 1108 2 0.5253971150561654 1108 3 0.5662541795989462 1108 4 0.6475369121344201 1108 5 0.5925762635434921 1108 6 0.38062621990692236 1108 7 0.38773784760802243 1108 8 0.3465383342376356 1108 9 0.4426845799498125 1108 10-11 0.5386983329902932 1108 12-13 0.39772270753750405 1108 14-15 0.5858230053732498 1108 16-17 0.7252731416337994 1108 18-19 0.8245615170675222 1108 20-21 0.5223967825024758 1108 22-23 0.7210940054606496 1108 24-25 0.6568070854906978 1108 26-27 0.4995886255926223 1108 28-29 0.6322231106823111 1108 30-31 0.6821218338903776 1108 32-33 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2216 2 -0.7371648347212769 2216 3 -0.908531309775114 2216 4 -1.0391723916329951 2216 5 -1.0251006679313477 2216 6 -0.8980290291837107 2216 7 -1.1351199982875215 2216 8 -0.974532256599602 2216 9 -1.2279891033208798 2216 10-11 -1.211559857401646 2216 12-13 -1.4177702526909428 2216 14-15 -1.8225358337196838 2216 16-17 -1.59165825513643 2216 18-19 -3.567719456153746 2216 20-21 -1.7868199494021368 2216 22-23 -1.2384995392717357 2216 24-25 -1.3870691845043694 2216 26-27 -1.3422914267988446 2216 28-29 -1.357008743152754 2216 30-31 -1.4982819852809968 2216 32-33 -1.44640573254118 2216 34-35 -1.7287401719727171 2216 36-37 -1.7056000063714336 2216 38-39 -1.8849975974225472 2216 40-41 -1.832837864431184 2216 42-43 -1.7299487310468535 2216 44-45 -1.6355020347082458 2216 46-47 -1.8977292284072362 2216 48-49 -1.8361777137492794 2216 50-51 -1.6734391590229976 2216 52-53 -1.659119499734416 2216 54-55 -1.6321476664543368 2216 56-57 -1.6699221376524136 2216 58-59 -1.7344880351830554 2216 60-61 -1.6458502549078204 2216 62-63 -1.6674904363172125 2216 64-65 -1.6395112132076655 2216 66-67 -1.6460445323410227 2216 68-69 -1.7437641387461724 2216 70-71 -1.8552073861248317 2216 72-73 -1.8200614366427565 2216 74-75 -1.844806822984328 2216 76-77 -1.6841879720912942 2216 78-79 -1.7946343401094822 2216 80-81 -1.78027265813229 2216 82-83 -1.995922281425777 2216 84-85 -1.8283526243092112 2216 86-87 -1.8164582959285944 2216 88-89 -1.76344302755205 2216 90-91 -1.721647876458892 2216 92-93 -1.6982800175297754 2216 94-95 -1.689334587034864 2216 96-97 -1.6396640483508556 2216 98-99 -1.562784317801185 2216 100 -1.670519368936887 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19604.0 1 3101.0 2 524.0 3 528.0 4 457.0 5 284.0 6 220.0 7 345.0 8 665.0 9 1184.0 10 2299.0 11 5383.0 12 9829.0 13 13665.0 14 17035.0 15 20301.0 16 23216.0 17 26690.0 18 29894.0 19 33555.0 20 37232.0 21 41628.0 22 47016.0 23 53750.0 24 59272.0 25 66891.0 26 75826.0 27 87126.0 28 100473.0 29 117441.0 30 139336.0 31 165085.0 32 201068.0 33 249279.0 34 336018.0 35 443185.0 36 645053.0 37 916904.0 38 679262.0 39 197467.0 40 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.919741281184635 20.666139337992995 12.339553197858406 24.074566182963963 2 15.512466231229888 19.534039456695716 40.70076052201247 24.252733790061924 3 18.975352471666675 26.400073142230678 28.3203662725159 26.304208113586746 4 12.389624137571694 16.323958353753724 39.66692495235816 31.619492556316427 5 13.341337426147803 38.373580717562795 34.781248968544745 13.503832887744661 6 30.358845211355366 38.06340774472615 17.896132260074495 13.681614783843987 7 27.41244588795972 31.267557835968397 23.470056955044715 17.84993932102717 8 26.408419594139332 34.9141162753828 19.597277087104146 19.080187043373716 9 26.197546004670148 15.038102375746615 21.345526919416212 37.41882470016702 10-11 26.10260730590779 25.45166872120803 27.10367484146994 21.34204913141425 12-13 26.30148447580616 23.13677565652121 27.220421107708482 23.341318759964146 14-15 23.602536027910308 24.37206611363768 24.653869934776086 27.37152792367593 16-17 22.662495317866775 27.037600369036756 25.973222947181405 24.32668136591506 18-19 22.075043883349686 27.873768339275646 27.42978302700852 22.62140475036615 20-21 23.847465011501086 26.902315084389492 26.319951187482193 22.93026871662723 22-23 24.34536402722077 26.448409954528852 26.135261711969253 23.070964306281127 24-25 23.86461959931437 26.500212104853404 26.74137878168913 22.893789514143094 26-27 23.642199070678842 26.75091531951363 25.827591179200727 23.7792944306068 28-29 22.688060038744332 27.451587393424447 26.410329646803532 23.450022921027692 30-31 23.555736135851436 26.92420210899067 26.312090649106334 23.20797110605156 32-33 23.915930110230292 27.083570977971387 25.423164021615175 23.577334890183145 34-35 23.978168917602563 26.90083227826897 26.316989273910952 22.804009530217513 36-37 23.585903989858842 26.404564925069973 26.01660949048244 23.99292159458874 38-39 23.77781643950607 26.576215430951216 26.57774354436614 23.068224585176576 40-41 23.366332237614827 25.759373194632957 27.240260762762574 23.63403380498964 42-43 23.321807904027697 25.93298000890246 26.550638665452553 24.19457342161729 44-45 23.590972001855025 26.708858759580146 26.42453301401503 23.275636224549803 46-47 23.573926027504765 26.61116010301379 25.927320340267695 23.887593529213746 48-49 24.18393522829931 26.222673832277106 26.174928560168798 23.418462379254787 50-51 23.660863028234054 26.04458419257682 26.387989048627098 23.90656373056203 52-53 23.30616255677441 26.56635634703683 26.05229202710429 24.07518906908447 54-55 23.148978744757432 26.438234243387292 26.05823973633835 24.354547275516925 56-57 24.302366432443666 25.901275743306073 25.7968711237346 23.999486700515664 58-59 23.23987213449129 26.277101266369062 26.677656635970877 23.805369963168772 60-61 23.173037931088594 26.964928588595484 26.14169256550003 23.720340914815893 62-63 23.621037279379436 26.30841926606935 25.616848752087478 24.45369470246374 64-65 22.809902747692327 26.495748379825102 26.069300980390235 24.62504789209234 66-67 23.57113543833132 26.022272363345223 26.013011569797424 24.39358062852603 68-69 22.883542482186105 26.13307377149058 26.391943752238223 24.591439994085096 70-71 22.85561363603278 25.8119499632599 26.49975432608584 24.832682074621477 72-73 23.48773415459438 25.830911441996236 26.899028503909232 23.782325899500147 74-75 23.533446278253567 26.290657615930215 26.441222163291343 23.73467394252488 76-77 23.283527452628288 26.097621480743626 25.982639568870482 24.636211497757603 78-79 23.25656226862059 26.115459165180503 26.04661201164423 24.58136655455468 80-81 22.842328410709644 26.440380008956982 26.816478287163992 23.90081329316938 82-83 23.660675801918966 25.398439186488957 27.85018601932883 23.090698992263246 84-85 23.70633629789274 25.956925559957707 26.527329380380966 23.80940876176859 86-87 22.440267470494916 26.661142522303187 26.96566434619463 23.932925661007268 88-89 23.497562317952656 25.35635848204319 26.69638267714082 24.449696522863327 90-91 23.04250201563604 25.44684301816383 26.854782313903453 24.655872652296683 92-93 23.455008696981224 25.67048575173649 26.86252041340149 24.011985137880796 94-95 22.32855467244381 25.620782190752134 27.52697861149138 24.52368452531267 96-97 22.554110486521957 25.456203886942674 27.200323531182963 24.789362095352406 98-99 21.99766809979204 26.156559170150846 27.01978792790005 24.82598480215706 100 22.71777227763889 25.21748158237888 26.30044600958464 25.764300130397586 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16075.0 1 9407.0 2 2554.0 3 1835.0 4 908.0 5 431.0 6 332.5 7 309.5 8 297.5 9 295.0 10 284.5 11 273.0 12 253.0 13 250.0 14 266.5 15 295.5 16 327.5 17 349.0 18 425.0 19 536.5 20 677.0 21 894.0 22 1212.0 23 1687.0 24 2341.0 25 3384.5 26 4950.0 27 6887.0 28 10497.0 29 18153.0 30 29328.5 31 40682.0 32 55542.5 33 70148.0 34 82509.0 35 95909.5 36 108572.5 37 129741.0 38 140287.0 39 133892.5 40 125552.0 41 117891.0 42 118319.5 43 127315.0 44 141248.5 45 168238.0 46 197986.5 47 222550.5 48 242673.0 49 241416.0 50 228243.5 51 232838.5 52 237674.0 53 230510.0 54 220182.5 55 192307.5 56 168405.5 57 141701.0 58 114002.5 59 106902.0 60 89793.5 61 65157.0 62 49961.0 63 40586.0 64 29635.0 65 16480.5 66 10139.5 67 7217.0 68 5137.5 69 3629.0 70 2728.5 71 1974.0 72 1359.0 73 1006.0 74 726.0 75 517.5 76 371.5 77 227.0 78 135.0 79 88.5 80 71.0 81 61.0 82 40.5 83 33.0 84 27.5 85 19.0 86 21.0 87 21.5 88 15.0 89 11.0 90 8.5 91 9.5 92 10.5 93 8.0 94 6.5 95 5.0 96 4.0 97 5.5 98 8.0 99 4.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9792134371007887 2 0.4366918074119082 3 0.46914738179032756 4 0.4547477883477504 5 0.42465448678801354 6 0.47270106176973675 7 0.48983267508087697 8 0.4923592799217286 9 0.5066150828449077 10-11 0.5189399845075986 12-13 0.5177280358441008 14-15 0.5157457808266845 16-17 0.5200081426516985 18-19 0.5184675299438621 20-21 0.5170912492581949 22-23 0.4799111292424109 24-25 0.5149652037213808 26-27 0.49545077608878696 28-29 0.5009558988314555 30-31 0.5042733515289965 32-33 0.5063788555630395 34-35 0.5193200023088649 36-37 0.5369959654434407 38-39 0.5262835717482852 40-41 0.5312135324133616 42-43 0.5281733900032312 44-45 0.5384235998860357 46-47 0.5366056768907888 48-49 0.5090492509232892 50-51 0.5240650894490009 52-53 0.5160436326168663 54-55 0.5263349255052131 56-57 0.5138765040745097 58-59 0.519535688087962 60-61 0.5133116127483031 62-63 0.5078783852653336 64-65 0.5191762117894669 66-67 0.5175534330705458 68-69 0.5379203330681426 70-71 0.5215795676136916 72-73 0.5250202693278594 74-75 0.5221547296912838 76-77 0.5098914525369064 78-79 0.5404058549034518 80-81 0.5144413954007164 82-83 0.5138148795661963 84-85 0.5234693858686374 86-87 0.827740395666318 88-89 1.0921506193365793 90-91 1.0828555893326333 92-93 1.0831328996200438 94-95 1.0865838720855974 96-97 1.0801338402154557 98-99 1.0759228321473697 100 1.0713009940238607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4868193.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.270845806606246 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61754149345369 22.587360930163804 2 11.981157651602446 7.253595517125966 3 4.862943629486462 4.416162503232085 4 2.5602897048169533 3.1000853949902165 5 1.4800930090766928 2.2401833628598213 6 0.9374566762778234 1.7026563898787748 7 0.6257780856732551 1.3259982358397835 8 0.4504626992293286 1.090870952799892 9 0.31975612545328097 0.8711359532382699 >10 1.7167678497152228 9.447339176300439 >50 0.17153607183584021 3.6597834684384942 >100 0.2046469833290859 13.913256865002332 >500 0.04008462807385316 8.539886400187624 >1k 0.029860339486780928 15.854489204478877 >5k 0.0012187893668073849 2.3512819107211174 >10k+ 4.062631222691283E-4 1.645913734742557 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 18868 0.3875770742860852 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 15236 0.3129703362212632 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 14517 0.2982009957288053 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11017 0.22630573602977533 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 10495 0.21558307158323428 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10169 0.2088865416798389 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 9429 0.19368582962918685 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8140 0.16720783255717264 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8049 0.16533855580499787 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7821 0.16065509317317533 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6865 0.14101741652395458 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6696 0.13754590255562998 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 6345 0.13033583508295582 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 6273 0.12885684688343294 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6136 0.1260426610037852 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5754 0.11819580694520533 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5692 0.11692223377339395 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5682 0.11671681874568243 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5465 0.11225931264434257 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 5437 0.11168415056675032 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5420 0.11133494501964075 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5266 0.10817155359288343 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5245 0.10774018203468926 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 5001 0.1027280553585283 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 4951 0.10170098021997075 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.054150277115143E-5 0.0 5 0.0 0.0 0.0 2.054150277115143E-5 4.108300554230286E-5 6 0.0 0.0 0.0 2.054150277115143E-5 6.16245083134543E-5 7 0.0 0.0 0.0 2.054150277115143E-5 6.16245083134543E-5 8 0.0 0.0 0.0 2.054150277115143E-5 6.16245083134543E-5 9 0.0 0.0 0.0 2.054150277115143E-5 6.16245083134543E-5 10-11 0.0 0.0 0.0 2.054150277115143E-5 6.16245083134543E-5 12-13 0.0 0.0 0.0 2.054150277115143E-5 1.8487352494036288E-4 14-15 0.0 0.0 0.0 2.054150277115143E-5 2.0541502771151431E-4 16-17 0.0 0.0 0.0 2.054150277115143E-5 2.2595653048266575E-4 18-19 0.0 0.0 0.0 2.054150277115143E-5 2.3622728186824146E-4 20-21 0.0 0.0 0.0 2.054150277115143E-5 3.286640443384229E-4 22-23 0.0 0.0 0.0 8.216601108460573E-5 3.286640443384229E-4 24-25 0.0 0.0 0.0 1.4379051939806002E-4 3.286640443384229E-4 26-27 0.0 0.0 0.0 3.4920554710957433E-4 4.1083005542302863E-4 28-29 0.0 0.0 0.0 0.0012119486634979346 5.0326681789321E-4 30-31 0.0 0.0 0.0 0.005155917195559009 5.135375692787858E-4 32-33 0.0 0.0 0.0 0.016268870194751935 5.238083206643616E-4 34-35 0.0 0.0 0.0 0.03367779379330277 5.340790720499372E-4 36-37 0.0 0.0 0.0 0.06295970599357914 5.546205748210886E-4 38-39 0.0 0.0 0.0 0.11370748858970875 6.059743317489673E-4 40-41 0.0 0.0 0.0 0.17012472595067615 6.162450831345429E-4 42-43 0.0 0.0 0.0 0.23203681530292658 6.470573372912701E-4 44-45 0.0 0.0 0.0 0.30649976284835045 6.984110942191487E-4 46-47 0.0 0.0 0.0 0.404503272569514 7.394940997614515E-4 48-49 0.0 0.0 0.0 0.5174815378108468 7.60035602532603E-4 50-51 0.0 0.0 0.0 0.6433290545383061 8.216601108460573E-4 52-53 0.0 0.0 0.0 0.7707377254763729 8.422016136172087E-4 54-55 0.0 0.0 0.0 0.911765412751713 8.627431163883601E-4 56-57 0.0 0.0 0.0 1.0850535301291466 8.832846191595116E-4 58-59 0.0 0.0 0.0 1.2951520204724833 8.832846191595116E-4 60-61 0.0 0.0 0.0 1.510919965580658 8.832846191595116E-4 62-63 0.0 0.0 0.0 1.742915697878042 8.832846191595116E-4 64-65 0.0 0.0 0.0 1.9873801223575156 9.03826121930663E-4 66-67 0.0 0.0 0.0 2.2704728427981387 9.03826121930663E-4 68-69 0.0 0.0 0.0 2.5911359718071982 9.243676247018144E-4 70-71 0.0 0.0 0.0 2.9498111516942735 9.346383760873902E-4 72-73 0.0 0.0 0.0 3.3295721841759356 0.00100653363578642 74-75 0.0 0.0 0.0 3.7048654398048724 0.001047616641328723 76-77 0.0 0.0 0.0 4.134850446562 0.001047616641328723 78-79 0.0 0.0 0.0 4.602118280848767 0.001047616641328723 80-81 0.0 0.0 0.0 5.109976124611329 0.001047616641328723 82-83 0.0 0.0 0.0 5.631668670490262 0.0011092411496421773 84-85 0.0 0.0 0.0 6.145401384045373 0.0011092411496421773 86-87 0.0 0.0 0.0 6.690644762851432 0.0011811364093412074 88 0.0 0.0 0.0 7.135912647670295 0.0012530316690402372 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 5290 0.0 37.747723 94 GTACTAG 5245 0.0 35.88169 1 TAGGCAT 6320 0.0 30.997656 5 GCATCGT 5800 0.0 30.114046 8 CTAACGC 2935 0.0 28.49693 3 CTAGGCA 7265 0.0 28.453808 4 TCTAACG 2970 0.0 28.319023 2 TAGTACT 7725 0.0 28.21908 4 TAACGCC 2990 0.0 28.12582 4 TATTCTC 2260 0.0 28.06307 5 CGCCTAC 3020 0.0 27.518232 7 CTGTGCG 7920 0.0 27.331364 9 CGTTAAC 920 0.0 26.660051 1 ACTGTGC 8790 0.0 25.852982 8 GTACATG 20180 0.0 25.640762 1 GTACTGT 8475 0.0 25.592169 6 ACATGGG 19495 0.0 25.404085 3 AGGGCTA 4905 0.0 25.28569 5 CTAGTAC 9070 0.0 25.177649 3 TACATGG 20165 0.0 25.142294 2 >>END_MODULE