FastQCFastQC Report
Mon 16 Jan 2017
ERR1146445_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146445_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447833
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135600.3048675613495381No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC130790.294053306407862No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT106700.2398921002654551No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA83890.18860870001189345No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC79930.1797054880432786No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA76300.17154421040538168No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC73150.16446210997580168No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC63300.14231649434679763No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG58780.13215424230181305No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT52410.11783266143310686No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC49780.11191966964587023No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT47400.1065687493212987No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT47070.1058268149905808No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT46670.10492750065031668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73900.032.039891
GTACTAG39800.031.6417081
CAACGCA67950.030.9739725
ATCAACG72650.028.8424513
TAGGCAT49550.028.5385045
GGGCACC44850.028.09904194
GCATCGT41650.028.067768
GGTATCA48450.027.5501771
TATCAAC88350.026.643042
GTACATG157650.026.0289731
TACATGG159000.025.7674792
ACATGGG159000.025.2048933
TAGTACT54850.025.0954194
CTAGGCA57500.025.0826824
AACGCAG87650.024.9043586
CTGTGCG52900.024.8519339
ACGCAGA85750.024.1989697
GCTATTG41000.024.0468888
CTAACGC20850.023.8854123
GTGATCG25500.023.56648
Produced by FastQC (version 0.11.5)