##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146445_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4447833
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.028396524779595	31.0	30.0	34.0	26.0	34.0
2	30.138053294716777	31.0	30.0	34.0	26.0	34.0
3	30.344544185899068	31.0	30.0	34.0	26.0	34.0
4	33.794016996591374	35.0	35.0	37.0	28.0	37.0
5	33.98057413576454	35.0	35.0	37.0	28.0	37.0
6	34.198290943027764	37.0	35.0	37.0	30.0	37.0
7	34.08814809368967	37.0	35.0	37.0	29.0	37.0
8	34.047888263790476	37.0	35.0	37.0	29.0	37.0
9	35.38647404252813	39.0	35.0	39.0	28.0	39.0
10-11	35.30972891743013	38.5	35.0	39.0	28.0	39.0
12-13	35.297294322875885	38.0	35.0	39.0	28.0	39.0
14-15	36.26224309680691	39.0	35.5	41.0	27.0	41.0
16-17	36.06816611594905	39.0	35.0	41.0	27.0	41.0
18-19	36.00287364656002	39.0	35.0	41.0	27.0	41.0
20-21	36.326982263048095	39.0	35.0	41.0	27.0	41.0
22-23	36.032741224771705	39.0	34.5	41.0	27.0	41.0
24-25	36.126325561233976	39.0	35.0	41.0	27.0	41.0
26-27	36.181718378365375	39.0	35.0	41.0	27.0	41.0
28-29	36.00194296863214	39.0	35.0	41.0	27.0	41.0
30-31	35.93157589324959	39.0	34.5	41.0	27.0	41.0
32-33	36.026017838349595	39.0	35.0	41.0	27.0	41.0
34-35	36.06988043391018	39.0	35.0	41.0	27.0	41.0
36-37	35.990735825738064	39.0	35.0	41.0	27.0	41.0
38-39	35.83450727579026	39.0	34.0	41.0	27.0	41.0
40-41	35.80102535324505	39.0	34.0	41.0	27.0	41.0
42-43	35.64055395065417	39.0	34.0	41.0	26.5	41.0
44-45	35.46739243582212	38.0	34.0	40.0	26.0	41.0
46-47	35.343187457802486	38.0	33.5	40.0	26.0	41.0
48-49	35.22503003147825	38.0	33.0	40.0	26.0	41.0
50-51	35.02091827638313	38.0	33.0	40.0	25.5	41.0
52-53	34.84147595019867	38.0	33.0	40.0	25.0	41.0
54-55	34.648966586650175	37.0	33.0	40.0	25.0	41.0
56-57	34.50783999758984	37.0	33.0	40.0	25.0	41.0
58-59	34.264315341875474	36.5	32.5	40.0	25.0	41.0
60-61	34.01100401026747	36.0	32.0	39.5	24.5	41.0
62-63	33.744467586800134	35.5	32.0	39.0	24.0	41.0
64-65	33.460948736159835	35.0	31.5	39.0	23.5	41.0
66-67	33.1691216824013	35.0	31.0	38.5	23.0	40.0
68-69	32.87720717032317	35.0	31.0	38.0	23.0	40.0
70-71	32.80158382745036	35.0	31.0	37.0	23.5	40.0
72-73	32.58101057301387	35.0	31.0	37.0	23.0	39.5
74-75	32.31789176886812	35.0	31.0	36.0	23.0	39.0
76-77	32.081373783593044	35.0	31.0	36.0	23.0	39.0
78-79	31.86170276626843	35.0	30.5	36.0	23.0	38.5
80-81	31.634537425303513	34.0	30.0	35.0	22.5	37.0
82-83	31.413447627192838	34.0	30.0	35.0	22.0	37.0
84-85	31.23231279142	34.0	30.0	35.0	21.5	36.5
86-87	31.07873609463305	34.0	30.0	35.0	21.0	36.0
88-89	31.085888566409757	34.0	30.0	35.0	21.0	36.0
90-91	31.019214862608376	34.0	30.0	35.0	21.0	36.0
92-93	30.891084602322074	34.0	30.0	35.0	20.0	35.5
94-95	30.86402288485202	34.0	30.0	35.0	20.0	35.0
96-97	30.780496255142673	34.0	30.0	35.0	20.0	35.0
98-99	30.70699461962713	34.0	30.0	35.0	20.0	35.0
100	30.193978730766197	34.0	29.0	35.0	19.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7888993447147534
1101	2	1.021496825316671
1101	3	0.9722186431202964
1101	4	1.0396629742711454
1101	5	0.9680147128304242
1101	6	0.6727022857337275
1101	7	0.7373037021253808
1101	8	0.7673566880228719
1101	9	1.0255387859585596
1101	10-11	1.0721230931307488
1101	12-13	0.8696325523573094
1101	14-15	1.183070270157728
1101	16-17	1.2663268284145204
1101	18-19	1.4318084821250778
1101	20-21	1.068114123178752
1101	22-23	1.4100184713752384
1101	24-25	1.1860334012652487
1101	26-27	1.095933494291792
1101	28-29	1.2400198099373014
1101	30-31	1.2754716947627074
1101	32-33	1.1297762475229476
1101	34-35	1.052096942250813
1101	36-37	0.9756898780351335
1101	38-39	1.0738453976918905
1101	40-41	0.9671191333132683
1101	42-43	1.0291788076013404
1101	44-45	1.0767007112509432
1101	46-47	1.079292448938638
1101	48-49	1.0323482985048216
1101	50-51	1.0413640947318044
1101	52-53	1.0217957292610507
1101	54-55	1.1053289858058903
1101	56-57	0.9670938246463621
1101	58-59	0.8765271222491648
1101	60-61	0.9506087661408671
1101	62-63	0.8597551312730616
1101	64-65	0.9018532601346791
1101	66-67	0.9288716782778579
1101	68-69	0.9191621093236151
1101	70-71	0.7756336508748305
1101	72-73	0.7896309772234176
1101	74-75	0.8027340045891052
1101	76-77	0.7951536136312001
1101	78-79	0.7982979293686618
1101	80-81	0.8320963768186651
1101	82-83	0.7850515592852894
1101	84-85	0.8522835436031997
1101	86-87	0.7863415114214334
1101	88-89	0.8370256892230863
1101	90-91	0.8352642308238813
1101	92-93	0.8994336575060586
1101	94-95	0.8377396144335485
1101	96-97	0.7710666278805292
1101	98-99	0.8217434138455708
1101	100	0.9463895304646854
1102	1	0.8080757316260048
1102	2	1.0343814596135203
1102	3	1.0037249376518496
1102	4	1.116934750318194
1102	5	0.9460459539870243
1102	6	0.7451584475631563
1102	7	0.789372322191582
1102	8	0.776994111179377
1102	9	0.9988233267595064
1102	10-11	0.985742461897523
1102	12-13	1.0136249985025998
1102	14-15	1.2107508128971318
1102	16-17	1.3652396895667707
1102	18-19	1.3823713931670198
1102	20-21	1.1598037463374524
1102	22-23	1.4214842829207797
1102	24-25	1.3156928062261812
1102	26-27	1.1401931664324039
1102	28-29	1.3101484258598006
1102	30-31	1.3543639983174884
1102	32-33	1.3007022321439834
1102	34-35	1.0785414451130464
1102	36-37	1.0404796477211917
1102	38-39	1.173425667693735
1102	40-41	1.0971458408602075
1102	42-43	1.1377393778491722
1102	44-45	1.185063108312427
1102	46-47	1.0892754449111877
1102	48-49	1.025095264094908
1102	50-51	1.202301274622954
1102	52-53	1.0734889125511629
1102	54-55	1.122205529372195
1102	56-57	1.0263435547417714
1102	58-59	1.0393170738014206
1102	60-61	1.07100524875883
1102	62-63	1.0250503072332506
1102	64-65	0.9332601882492
1102	66-67	1.05192615948134
1102	68-69	1.0652729475153322
1102	70-71	1.0072038147196096
1102	72-73	0.9052769094953916
1102	74-75	0.8837981947927531
1102	76-77	0.8910435955356633
1102	78-79	0.966702305412408
1102	80-81	0.9498112495069115
1102	82-83	0.9755642104885887
1102	84-85	0.9770171416408999
1102	86-87	0.9622256370949991
1102	88-89	0.9050996743424804
1102	90-91	0.997964706285142
1102	92-93	1.0155420421249985
1102	94-95	0.9574712162657342
1102	96-97	0.957307565254176
1102	98-99	0.9196399547440812
1102	100	1.0193146384844134
1103	1	0.6620960825811153
1103	2	0.8846157087152271
1103	3	0.7734751931342174
1103	4	0.8915770200350153
1103	5	0.8860011976536768
1103	6	0.6060603713881889
1103	7	0.6992849281400098
1103	8	0.7106593726603023
1103	9	0.8069388712223855
1103	10-11	0.8824911788318559
1103	12-13	0.7598971526755847
1103	14-15	0.9198482899233511
1103	16-17	1.206377350942347
1103	18-19	1.2748729071366114
1103	20-21	0.9789923037898447
1103	22-23	1.1566595720159398
1103	24-25	1.0607113417527145
1103	26-27	0.9951880469693677
1103	28-29	1.1284938757922376
1103	30-31	1.1834316097807474
1103	32-33	1.0249099776689405
1103	34-35	1.0112312347073953
1103	36-37	0.9701186276166496
1103	38-39	1.0410130374024433
1103	40-41	0.9578070156440717
1103	42-43	0.9352652134889325
1103	44-45	1.044975857569689
1103	46-47	1.0344375111153212
1103	48-49	1.018065042654861
1103	50-51	1.112761222196255
1103	52-53	1.0925676286829642
1103	54-55	1.0796512443749577
1103	56-57	0.9649989086737847
1103	58-59	0.9573432473359489
1103	60-61	0.9898215840666538
1103	62-63	0.8531818763732062
1103	64-65	0.8862283853077386
1103	66-67	0.9612560186680312
1103	68-69	0.9350561129901678
1103	70-71	0.8420264148618699
1103	72-73	0.8136212259137849
1103	74-75	0.8650145574588279
1103	76-77	0.8842865835713809
1103	78-79	0.8861058536137243
1103	80-81	0.8869612127491742
1103	82-83	0.7839437180621296
1103	84-85	0.7704522557940479
1103	86-87	0.8518961732051373
1103	88-89	0.8766194367497668
1103	90-91	0.8985093113000566
1103	92-93	0.8728279599148792
1103	94-95	0.8508427915966372
1103	96-97	0.8733708100773505
1103	98-99	0.9200031641365989
1103	100	0.9215849175495272
1104	1	0.6383699300426535
1104	2	0.8076374329693365
1104	3	0.7477013601932931
1104	4	0.7861928920040597
1104	5	0.7006946144196604
1104	6	0.48529903703889943
1104	7	0.4822712969216667
1104	8	0.4652501867939449
1104	9	0.6631862199351275
1104	10-11	0.6770350202468904
1104	12-13	0.548735696931324
1104	14-15	0.7483407765122791
1104	16-17	0.9277612898746668
1104	18-19	0.9127084605995535
1104	20-21	0.7458635319141962
1104	22-23	1.0129390417983615
1104	24-25	0.8975822503668951
1104	26-27	0.8166193777057913
1104	28-29	0.9657411772114273
1104	30-31	0.9996651940482906
1104	32-33	0.8235752168500738
1104	34-35	0.762669592589134
1104	36-37	0.8017884926286101
1104	38-39	0.8475406830718271
1104	40-41	0.7629547968443333
1104	42-43	0.7119793860209853
1104	44-45	0.7448936918734574
1104	46-47	0.666913805813131
1104	48-49	0.6759205440578668
1104	50-51	0.8201663928155369
1104	52-53	0.8089912619829391
1104	54-55	0.7252440739545634
1104	56-57	0.7002646953150915
1104	58-59	0.6140051864739675
1104	60-61	0.6988525342855567
1104	62-63	0.6994905221011081
1104	64-65	0.6346989169565944
1104	66-67	0.7553154460102007
1104	68-69	0.7293614649102125
1104	70-71	0.5607980133289203
1104	72-73	0.5404603616884671
1104	74-75	0.5097337789695544
1104	76-77	0.5792404277671537
1104	78-79	0.5680382696360731
1104	80-81	0.5133491665317926
1104	82-83	0.5427527277500914
1104	84-85	0.5006831781318581
1104	86-87	0.5200937048596472
1104	88-89	0.44408576626360485
1104	90-91	0.5076012594302739
1104	92-93	0.5026536144955145
1104	94-95	0.4254726109120277
1104	96-97	0.519205322364158
1104	98-99	0.45715239993006307
1104	100	0.6102421534827336
1105	1	0.45679447756185
1105	2	0.6436655114463576
1105	3	0.7088656433837954
1105	4	0.702322418258511
1105	5	0.6782215213524765
1105	6	0.4334607414506877
1105	7	0.508627204574509
1105	8	0.4780085509349803
1105	9	0.5324538472678526
1105	10-11	0.6096906280507426
1105	12-13	0.5591313816601726
1105	14-15	0.7702739204372051
1105	16-17	0.8864013986855284
1105	18-19	0.8700789766957087
1105	20-21	0.7467185355111496
1105	22-23	1.0307093486969308
1105	24-25	0.8672162333048021
1105	26-27	0.6902393589047762
1105	28-29	0.9105076270553454
1105	30-31	0.8874940404891163
1105	32-33	0.7667702359781359
1105	34-35	0.6935151070715548
1105	36-37	0.6418170812943842
1105	38-39	0.7496219697943332
1105	40-41	0.6602622519504067
1105	42-43	0.7103115237980404
1105	44-45	0.6851432551556584
1105	46-47	0.7150673186408554
1105	48-49	0.7900048493660208
1105	50-51	0.8348968219350894
1105	52-53	0.7332003035064716
1105	54-55	0.6934040110629311
1105	56-57	0.6548977511384209
1105	58-59	0.6392581091094698
1105	60-61	0.6936982763078205
1105	62-63	0.691315724988165
1105	64-65	0.616903457670162
1105	66-67	0.6876238742173939
1105	68-69	0.7384244095693333
1105	70-71	0.635052470288116
1105	72-73	0.5998427379346509
1105	74-75	0.5509321564975309
1105	76-77	0.609461699426646
1105	78-79	0.6416373624826299
1105	80-81	0.7136146471348503
1105	82-83	0.71995729155039
1105	84-85	0.6605598123524103
1105	86-87	0.6755849534269842
1105	88-89	0.6259124528704803
1105	90-91	0.6584384002518853
1105	92-93	0.6296050813414595
1105	94-95	0.561343379943434
1105	96-97	0.5651562767594136
1105	98-99	0.6372907640475418
1105	100	0.6994740208683545
1106	1	0.4826312923546503
1106	2	0.5919288547045234
1106	3	0.651803940233556
1106	4	0.6756644507412659
1106	5	0.6319452479604948
1106	6	0.44692824476639004
1106	7	0.5176287024877197
1106	8	0.5198940839956308
1106	9	0.7346793670335074
1106	10-11	0.674306345208656
1106	12-13	0.5900328651190065
1106	14-15	0.8418893839330792
1106	16-17	0.9426087495046787
1106	18-19	0.8761404386870382
1106	20-21	0.6741297883686386
1106	22-23	1.0267285311549585
1106	24-25	0.8746387939198996
1106	26-27	0.6583739691489754
1106	28-29	0.9104699187432104
1106	30-31	0.8672443642918424
1106	32-33	0.7446607566521593
1106	34-35	0.7224765968539373
1106	36-37	0.656976372154567
1106	38-39	0.765745489001894
1106	40-41	0.7124024757223921
1106	42-43	0.7012208463423093
1106	44-45	0.6403130448586509
1106	46-47	0.6796018601557066
1106	48-49	0.6168953088504665
1106	50-51	0.730289910013795
1106	52-53	0.7491869228809875
1106	54-55	0.7231936060694153
1106	56-57	0.6702629932246467
1106	58-59	0.6985510815720701
1106	60-61	0.6596101705191941
1106	62-63	0.5953649403502652
1106	64-65	0.6365025636582757
1106	66-67	0.7224392076806794
1106	68-69	0.6436763978989788
1106	70-71	0.6440882192077808
1106	72-73	0.5827742849870603
1106	74-75	0.6131424319131327
1106	76-77	0.6131343336870856
1106	78-79	0.7016994481150682
1106	80-81	0.6127512395044938
1106	82-83	0.5511312770016694
1106	84-85	0.4627103985693708
1106	86-87	0.5267220281144489
1106	88-89	0.582942178311157
1106	90-91	0.5203952788409687
1106	92-93	0.5594383331202692
1106	94-95	0.6076356516622745
1106	96-97	0.6064121160539955
1106	98-99	0.6533066331753581
1106	100	0.6198207668427713
1107	1	0.455009089352032
1107	2	0.6672815753912609
1107	3	0.5273555216719856
1107	4	0.653298606865043
1107	5	0.5888526919951147
1107	6	0.33537376820477505
1107	7	0.35913841654155476
1107	8	0.321107142116432
1107	9	0.3669105202393581
1107	10-11	0.462758801268933
1107	12-13	0.2792335451187853
1107	14-15	0.48902931005167005
1107	16-17	0.6207177996469397
1107	18-19	0.6784548417553253
1107	20-21	0.5210483698110195
1107	22-23	0.735532122629877
1107	24-25	0.42101797781843686
1107	26-27	0.40050758149521926
1107	28-29	0.553948732895627
1107	30-31	0.576423984568784
1107	32-33	0.5128978945192131
1107	34-35	0.36126865719336365
1107	36-37	0.35747561512356896
1107	38-39	0.41339237251519734
1107	40-41	0.28337831949123427
1107	42-43	0.454715509447972
1107	44-45	0.30958642131113123
1107	46-47	0.396578551286872
1107	48-49	0.4740211022165184
1107	50-51	0.4532158268483286
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2210	22-23	-1.7634939175098197
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2212	94-95	-0.310047086984067
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2212	98-99	-0.45533026353190564
2212	100	-0.49872387622633596
2213	1	-0.6936061602889616
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2213	14-15	-0.7284940432456182
2213	16-17	-0.5969166751561943
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2213	22-23	-0.9027003836889662
2213	24-25	-0.6527215624240341
2213	26-27	-3.3251007377765944
2213	28-29	-1.1673946886283844
2213	30-31	-1.564747245599527
2213	32-33	-1.0809549009559873
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2213	60-61	-0.868400753119424
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2213	64-65	-0.79757734269932
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2213	68-69	-0.7378496513064903
2213	70-71	-0.8711898504387605
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2213	86-87	-0.6835237439346251
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2213	94-95	-0.8527087120190799
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2213	100	-0.5973932928739067
2214	1	-0.9157979103054394
2214	2	-0.9051132280278296
2214	3	-1.159664270078487
2214	4	-0.8378011158692047
2214	5	-1.2019387561187287
2214	6	-0.9630171259643561
2214	7	-1.1508412000021835
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2214	12-13	-1.303800887744302
2214	14-15	-3.5798191533914405
2214	16-17	-3.1378671408768994
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2214	22-23	-2.7353952223550095
2214	24-25	-2.142358054283349
2214	26-27	-1.409871728811133
2214	28-29	-1.0018149190137606
2214	30-31	-1.0682288530672608
2214	32-33	-1.2526389484116436
2214	34-35	-1.074857811311574
2214	36-37	-1.0795318933289764
2214	38-39	-1.0301510901318238
2214	40-41	-1.2584035198810781
2214	42-43	-4.027163619842451
2214	44-45	-1.8244830483111443
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2214	52-53	-1.1654893763063399
2214	54-55	-1.101318331355749
2214	56-57	-1.0229898843412855
2214	58-59	-1.208387008335471
2214	60-61	-1.4027629111202558
2214	62-63	-1.3534963440420498
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2214	78-79	-1.0632398756805337
2214	80-81	-1.0484628228028363
2214	82-83	-1.1428596640428204
2214	84-85	-1.1450487771676947
2214	86-87	-1.0658481342068988
2214	88-89	-1.0070749764281288
2214	90-91	-0.9565723850177186
2214	92-93	-1.0827613213750098
2214	94-95	-1.2074358170460862
2214	96-97	-1.104447183353038
2214	98-99	-1.3151889753158166
2214	100	-1.2218825208342956
2215	1	-4.3856471934544246
2215	2	-2.3825889381419927
2215	3	-4.163448836482466
2215	4	-2.2851915743141156
2215	5	-1.8898029031614811
2215	6	-1.5593630583386329
2215	7	-1.4538908494264433
2215	8	-1.4409535403345473
2215	9	-2.0329445033176583
2215	10-11	-1.6353332216764755
2215	12-13	-1.621679370692
2215	14-15	-3.8297097537988023
2215	16-17	-3.5218480363911198
2215	18-19	-2.448093066986438
2215	20-21	-2.060794314395224
2215	22-23	-3.4287674885593304
2215	24-25	-5.81230510846898
2215	26-27	-3.8481918165984865
2215	28-29	-1.9656886131106788
2215	30-31	-4.194341573247883
2215	32-33	-4.209649224359993
2215	34-35	-3.228671516995348
2215	36-37	-2.2240622846667364
2215	38-39	-2.1653420246686323
2215	40-41	-2.2710326869278106
2215	42-43	-2.0677558392134614
2215	44-45	-3.609279707879935
2215	46-47	-2.962243080584912
2215	48-49	-2.222779404016059
2215	50-51	-5.525305315633005
2215	52-53	-6.026188937723568
2215	54-55	-3.4225221788269238
2215	56-57	-2.14579153322979
2215	58-59	-1.8527008917693308
2215	60-61	-3.399217632078795
2215	62-63	-2.942556729077218
2215	64-65	-1.9963922555039595
2215	66-67	-1.7904691255955463
2215	68-69	-2.0000398817558533
2215	70-71	-1.7289017912245939
2215	72-73	-1.5819589731966204
2215	74-75	-1.819982522905331
2215	76-77	-1.8529550447233625
2215	78-79	-1.7523055755186014
2215	80-81	-1.7611549916007796
2215	82-83	-1.7513917702773902
2215	84-85	-1.6205769223708266
2215	86-87	-1.5966138600710131
2215	88-89	-1.5634793890917322
2215	90-91	-1.9276029388788558
2215	92-93	-3.9942031270230416
2215	94-95	-2.2820603172497336
2215	96-97	-1.9171813759484024
2215	98-99	-3.40850937369909
2215	100	-3.451713486340495
2216	1	-0.8876463572907589
2216	2	-0.8037678388673832
2216	3	-1.1187702954036567
2216	4	-1.089180607853109
2216	5	-1.109398921029161
2216	6	-1.0186633870746675
2216	7	-1.3546214534421352
2216	8	-1.1217597481497776
2216	9	-1.416440595001852
2216	10-11	-1.4280176098569797
2216	12-13	-1.7715058164678865
2216	14-15	-2.0878698736518118
2216	16-17	-1.7866697435156382
2216	18-19	-3.6554862481266355
2216	20-21	-1.9711026403416625
2216	22-23	-1.4355466093096112
2216	24-25	-1.5559617697263022
2216	26-27	-1.4745185973517252
2216	28-29	-1.4846184568329406
2216	30-31	-1.651610760653817
2216	32-33	-1.5777820575739554
2216	34-35	-1.7656868074179073
2216	36-37	-1.8232939675605238
2216	38-39	-1.9620941073224003
2216	40-41	-1.9538011009841156
2216	42-43	-1.9202179890545068
2216	44-45	-1.796712441818734
2216	46-47	-1.9889111385963432
2216	48-49	-2.0374075378335803
2216	50-51	-1.925843484210091
2216	52-53	-1.850231884021916
2216	54-55	-1.7539955290549827
2216	56-57	-1.7769084315374712
2216	58-59	-1.871967192998305
2216	60-61	-1.8592603813933124
2216	62-63	-1.7522728947456727
2216	64-65	-1.9213395203921344
2216	66-67	-1.9610713058993952
2216	68-69	-1.992076977005425
2216	70-71	-1.947174243048508
2216	72-73	-1.9904547426389527
2216	74-75	-1.9716396570718544
2216	76-77	-1.8833586876537254
2216	78-79	-2.0083558437687614
2216	80-81	-1.8157779473462128
2216	82-83	-2.024707079035359
2216	84-85	-1.870475331384128
2216	86-87	-1.8065219616261068
2216	88-89	-1.7542276679964104
2216	90-91	-1.7667985763222838
2216	92-93	-1.638712474164734
2216	94-95	-1.5657990092586083
2216	96-97	-1.6654194684640728
2216	98-99	-1.6676911544341344
2216	100	-1.6691371006804516
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18252.0
1	2786.0
2	494.0
3	476.0
4	396.0
5	230.0
6	230.0
7	257.0
8	499.0
9	868.0
10	2002.0
11	4775.0
12	9020.0
13	12559.0
14	15867.0
15	18816.0
16	21942.0
17	24522.0
18	27423.0
19	30415.0
20	33904.0
21	37598.0
22	42328.0
23	47916.0
24	53488.0
25	60124.0
26	68461.0
27	77802.0
28	90127.0
29	105487.0
30	123777.0
31	147020.0
32	178531.0
33	222511.0
34	296542.0
35	389722.0
36	568150.0
37	829884.0
38	687077.0
39	195425.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.32810232461607	20.06252686559335	12.096573307050372	24.512797502740206
2	15.74851623285631	19.68488875670239	40.06919918240089	24.497395828040418
3	19.033084663149907	25.820079595844497	28.50633204212792	26.640503698877676
4	12.395397962078542	16.076029938182927	39.1105913007967	32.41798079894183
5	13.47522518809989	38.07903616264259	34.91760772121227	13.52813092804526
6	31.04791750960069	37.47949566508822	17.85684997841411	13.615736846896976
7	27.715692273209157	31.276746601063405	23.147948979121498	17.859612146605944
8	26.387309084713806	35.03620029943597	19.838898386543548	18.737592229306674
9	26.339645171794984	14.930992067697895	20.91148368014886	37.81787908035826
10-11	25.888426062999066	25.551315286890524	27.497722911689653	21.062535738420753
12-13	25.936170037247575	23.19890674455573	27.564052074053976	23.30087114414272
14-15	23.30668286667896	24.563946655673803	24.731348289416115	27.398022188231124
16-17	22.73619163091751	27.242048668725598	26.00354822680761	24.01821147354928
18-19	22.266801897373455	27.75513824891302	27.412085443958834	22.56597440975469
20-21	23.700564496107017	26.93934579564931	26.51843440898321	22.84165529926046
22-23	24.012909076614008	26.563094473167432	26.30011422921936	23.1238822209992
24-25	23.469039637007587	26.714256704828877	26.862143590289485	22.95456006787406
26-27	23.43911949733853	26.868762234319117	26.028451069389362	23.663667198952993
28-29	22.768245939358494	27.289784208297498	26.571480351778394	23.370489500565615
30-31	23.45762360253388	27.014258493267572	26.485022435387528	23.043095468811014
32-33	23.685724635896193	27.162414014959857	25.724145505478702	23.427715843665247
34-35	23.700545042358847	26.9410671466285	26.522795644317004	22.835592166695655
36-37	23.4482090596635	26.579431507398187	26.206128440984987	23.76623099195332
38-39	23.627082134917934	26.764498801490422	26.65009510097203	22.958323962619613
40-41	23.314595979408463	25.999431968509402	27.206651461144038	23.4793205909381
42-43	23.217021103464955	26.158724095335717	26.678461762754697	23.94579303844463
44-45	23.411253182014747	26.84959234268397	26.565745663519447	23.173408811781833
46-47	23.331582194169837	26.665106163565287	26.204403602614683	23.79890803965019
48-49	23.813239209578313	26.45439631074173	26.383322133594824	23.34904234608514
50-51	23.48220931223384	26.273967302012586	26.454639583265998	23.789183802487575
52-53	23.137091114016798	26.69892451611248	26.290655812046104	23.873328557824617
54-55	23.04165089195405	26.62993371795379	26.347435455526615	23.98097993456555
56-57	23.892361204101057	26.25360711532136	26.102819327098192	23.751212353479392
58-59	23.027380515014368	26.52874231392468	26.823500215901152	23.6203769551598
60-61	23.055413081316082	27.02895841179775	26.30654904264656	23.609079464239606
62-63	23.428778110469345	26.51235774215232	25.935885706266383	24.122978441111957
64-65	22.723643702433773	26.60203417089788	26.342832933801585	24.331489192866762
66-67	23.299021286833394	26.329903032712405	26.263728105122308	24.107347575331893
68-69	22.809126770229504	26.358893478629184	26.524103784668927	24.307875966472377
70-71	22.72036943888599	26.078964352433793	26.6832103817896	24.517455826890615
72-73	23.209550870234548	26.091949242665507	26.959618395544393	23.73888149155555
74-75	23.33589639620703	26.35819730340471	26.621024257785358	23.684882042602897
76-77	23.10663153687707	26.270007544761498	26.33463011628786	24.288730802073573
78-79	23.003300300189643	26.287041342708335	26.333678230791335	24.375980126310687
80-81	22.655920525382978	26.547938846346998	26.949144996065233	23.846995632204795
82-83	23.3304611712884	25.71367388323277	27.733457335398278	23.222407610080552
84-85	23.448185063153666	26.13339541433133	26.659543173510553	23.758876349004453
86-87	22.384637925786706	26.624976690379658	27.122388401472996	23.86799698236064
88-89	23.275781229486984	25.5612134306353	26.982483168740433	24.18052217113729
90-91	22.885491676964644	25.7187549048701	27.04684455677477	24.348908861390488
92-93	23.11540643701822	25.831040546413703	27.103287247255476	23.9502657693126
94-95	22.289834890364503	25.791001157742866	27.561020186734623	24.358143765158008
96-97	22.51347256508752	25.64177166290839	27.278777679746813	24.56597809225728
98-99	22.02989369463973	26.17398439400035	27.158155646321593	24.637966265038333
100	22.66073939656487	25.59113791448976	26.511116358851822	25.237006330093546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14904.0
1	8772.5
2	2432.5
3	1731.0
4	862.5
5	417.0
6	322.0
7	299.0
8	292.5
9	290.5
10	282.0
11	255.0
12	237.0
13	245.0
14	263.0
15	322.0
16	364.0
17	384.0
18	478.5
19	614.5
20	771.5
21	1053.5
22	1505.0
23	2132.0
24	2941.5
25	4251.5
26	6260.0
27	8616.0
28	12142.0
29	18977.5
30	29036.5
31	39125.5
32	51415.0
33	64240.0
34	75929.5
35	88748.5
36	101323.5
37	120340.5
38	130748.0
39	128014.5
40	124287.5
41	119117.5
42	119832.5
43	129190.0
44	141774.5
45	162608.5
46	183950.5
47	197168.5
48	205203.0
49	204119.0
50	198125.5
51	202737.5
52	205826.0
53	198830.0
54	189697.0
55	168431.0
56	149664.5
57	127918.0
58	105676.5
59	97777.0
60	81504.5
61	59761.0
62	45642.0
63	36633.5
64	26796.0
65	15934.5
66	10360.0
67	7407.0
68	5222.5
69	3644.5
70	2640.0
71	1945.0
72	1349.5
73	959.0
74	675.0
75	468.5
76	313.0
77	198.5
78	130.0
79	80.5
80	54.0
81	46.0
82	40.0
83	30.5
84	24.0
85	22.5
86	15.5
87	11.5
88	14.5
89	10.0
90	10.0
91	8.5
92	5.5
93	7.5
94	5.5
95	5.5
96	5.0
97	3.0
98	2.5
99	4.0
100	11.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.988481357101312
2	0.4439465240713849
3	0.47708625751011785
4	0.4626297794903721
5	0.4317158490437928
6	0.47955937194584414
7	0.49545475291001256
8	0.4986473188179502
9	0.5117548253272999
10-11	0.5243677089495042
12-13	0.5227152188492689
14-15	0.5203657601353288
16-17	0.5254131618700612
18-19	0.5237494303405725
20-21	0.5231199103023877
22-23	0.48664147237542416
24-25	0.5208716244517274
26-27	0.5006932589420511
28-29	0.5069210107483801
30-31	0.5099337137882649
32-33	0.5123843453654847
34-35	0.5241766046521981
36-37	0.5414209571267626
38-39	0.530325666453754
40-41	0.5346985824332883
42-43	0.5323491237193483
44-45	0.542781170066412
46-47	0.5406228156497782
48-49	0.514374078343319
50-51	0.5288867635093314
52-53	0.5210065216027671
54-55	0.529651180698556
56-57	0.5187132700350935
58-59	0.5250759189924622
60-61	0.5183760271574944
62-63	0.5129126925403898
64-65	0.5233672217459603
66-67	0.521523627348419
68-69	0.5413310256927363
70-71	0.5259527504742196
72-73	0.5298310435666087
74-75	0.5269757205362702
76-77	0.5150485640985172
78-79	0.546243530276429
80-81	0.5210402458905269
82-83	0.5210514873197802
84-85	0.5284708306269592
86-87	0.8341252920242284
88-89	1.1005246824689685
90-91	1.0905647761505435
92-93	1.0910706404669421
94-95	1.096174249347941
96-97	1.0888335960455349
98-99	1.0846405429340535
100	1.0804362483933188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4447833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.228369990735835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.05455570883751	29.074416951184705
2	12.068502148747678	9.227183307526332
3	4.62429567234336	5.303378577267013
4	2.238663286935707	3.423217936706201
5	1.329805088344661	2.541814046640143
6	0.7968740433813463	1.8277917459837472
7	0.5256581374903216	1.4066537638034773
8	0.3647105862497685	1.1153832984555458
9	0.2755241615488882	0.9479555630170351
>10	1.3994703843890577	9.779522704165771
>50	0.13143674898861013	3.524292111747724
>100	0.14725308326406222	12.279952206230897
>500	0.027582016644783075	7.4455646219716485
>1k	0.015082081417405848	10.171610157036637
>5k	4.1079599191377793E-4	1.0942589822971167
>10k+	1.7605542510590484E-4	0.8370040259661131
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	13560	0.3048675613495381	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13079	0.294053306407862	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	10670	0.2398921002654551	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8389	0.18860870001189345	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	7993	0.1797054880432786	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7630	0.17154421040538168	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	7315	0.16446210997580168	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	6330	0.14231649434679763	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5878	0.13215424230181305	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5241	0.11783266143310686	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4978	0.11191966964587023	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4740	0.1065687493212987	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4707	0.1058268149905808	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4667	0.10492750065031668	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2482858506603104E-5
2	0.0	0.0	0.0	0.0	2.2482858506603104E-5
3	0.0	0.0	0.0	0.0	2.2482858506603104E-5
4	0.0	0.0	0.0	0.0	2.2482858506603104E-5
5	2.2482858506603104E-5	0.0	0.0	0.0	2.2482858506603104E-5
6	2.2482858506603104E-5	0.0	0.0	0.0	2.2482858506603104E-5
7	2.2482858506603104E-5	0.0	0.0	0.0	2.2482858506603104E-5
8	2.2482858506603104E-5	0.0	0.0	0.0	4.496571701320621E-5
9	2.2482858506603104E-5	0.0	0.0	0.0	4.496571701320621E-5
10-11	2.2482858506603104E-5	0.0	0.0	2.2482858506603104E-5	4.496571701320621E-5
12-13	2.2482858506603104E-5	0.0	0.0	2.2482858506603104E-5	1.798628680528248E-4
14-15	2.2482858506603104E-5	0.0	0.0	2.2482858506603104E-5	2.3607001431933257E-4
16-17	2.2482858506603104E-5	0.0	0.0	2.2482858506603104E-5	2.6979430207923723E-4
18-19	3.3724287759904654E-5	0.0	0.0	2.2482858506603104E-5	2.6979430207923723E-4
20-21	4.496571701320621E-5	0.0	0.0	5.620714626650776E-5	2.810357313325388E-4
22-23	4.496571701320621E-5	0.0	0.0	7.869000477311086E-5	2.9227716058584033E-4
24-25	4.496571701320621E-5	1.1241429253301552E-5	0.0	1.798628680528248E-4	2.9227716058584033E-4
26-27	4.496571701320621E-5	2.2482858506603104E-5	0.0	4.0469145311885585E-4	2.9227716058584033E-4
28-29	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.0011691086423433615	2.9227716058584033E-4
30-31	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.006295200381848869	2.9227716058584033E-4
32-33	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.01817739110258861	2.9227716058584033E-4
34-35	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.037388993696480965	3.147600190924434E-4
36-37	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.07132686861219834	3.147600190924434E-4
38-39	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.12848953636523675	3.26001448345745E-4
40-41	4.496571701320621E-5	2.2482858506603104E-5	0.0	0.19221719880220323	3.3724287759904657E-4
42-43	4.496571701320621E-5	4.496571701320621E-5	0.0	0.2595083943124663	3.3724287759904657E-4
44-45	4.496571701320621E-5	4.496571701320621E-5	0.0	0.33956985345448	3.4848430685234814E-4
46-47	4.496571701320621E-5	4.496571701320621E-5	0.0	0.4449020455579155	3.5972573610564966E-4
48-49	4.496571701320621E-5	4.496571701320621E-5	0.0	0.5661633429132793	3.9345002386555433E-4
50-51	4.496571701320621E-5	4.496571701320621E-5	0.0	0.6984974480831452	4.2717431162545894E-4
52-53	4.496571701320621E-5	4.496571701320621E-5	0.0	0.8304718275169054	5.171057456518713E-4
54-55	4.496571701320621E-5	4.496571701320621E-5	0.0	0.9813880152424788	5.395886041584745E-4
56-57	4.496571701320621E-5	4.496571701320621E-5	0.0	1.1674673936723794	5.620714626650776E-4
58-59	4.496571701320621E-5	4.496571701320621E-5	0.0	1.3832803524772626	5.845543211716807E-4
60-61	4.496571701320621E-5	4.496571701320621E-5	0.0	1.6103459819647006	5.845543211716807E-4
62-63	4.496571701320621E-5	4.496571701320621E-5	0.0	1.8533744409918267	6.5200289669149E-4
64-65	4.496571701320621E-5	4.496571701320621E-5	0.0	2.1063403234788716	6.5200289669149E-4
66-67	4.496571701320621E-5	4.496571701320621E-5	0.0	2.397819342587727	6.5200289669149E-4
68-69	4.496571701320621E-5	4.496571701320621E-5	0.0	2.7333197986525124	6.5200289669149E-4
70-71	4.496571701320621E-5	4.496571701320621E-5	0.0	3.104972691195915	6.744857551980931E-4
72-73	4.496571701320621E-5	4.496571701320621E-5	0.0	3.488148048723952	6.857271844513946E-4
74-75	4.496571701320621E-5	4.496571701320621E-5	0.0	3.872638653474625	7.194514722112992E-4
76-77	4.496571701320621E-5	4.496571701320621E-5	0.0	4.305152643995402	7.419343307179024E-4
78-79	4.496571701320621E-5	4.496571701320621E-5	0.0	4.778248194120597	7.419343307179024E-4
80-81	4.496571701320621E-5	6.744857551980931E-5	0.0	5.289485913702245	7.419343307179024E-4
82-83	4.496571701320621E-5	6.744857551980931E-5	0.0	5.824926430466252	7.644171892245055E-4
84-85	4.496571701320621E-5	6.744857551980931E-5	0.0	6.352576636757719	7.869000477311087E-4
86-87	4.496571701320621E-5	6.744857551980931E-5	0.0	6.908397864757962	7.981414769844101E-4
88	4.496571701320621E-5	6.744857551980931E-5	0.0	7.35603157762443	8.093829062377117E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7390	0.0	32.03989	1
GTACTAG	3980	0.0	31.641708	1
CAACGCA	6795	0.0	30.973972	5
ATCAACG	7265	0.0	28.842451	3
TAGGCAT	4955	0.0	28.538504	5
GGGCACC	4485	0.0	28.099041	94
GCATCGT	4165	0.0	28.06776	8
GGTATCA	4845	0.0	27.550177	1
TATCAAC	8835	0.0	26.64304	2
GTACATG	15765	0.0	26.028973	1
TACATGG	15900	0.0	25.767479	2
ACATGGG	15900	0.0	25.204893	3
TAGTACT	5485	0.0	25.095419	4
CTAGGCA	5750	0.0	25.082682	4
AACGCAG	8765	0.0	24.904358	6
CTGTGCG	5290	0.0	24.851933	9
ACGCAGA	8575	0.0	24.198969	7
GCTATTG	4100	0.0	24.046888	8
CTAACGC	2085	0.0	23.885412	3
GTGATCG	2550	0.0	23.5664	8
>>END_MODULE