FastQCFastQC Report
Mon 16 Jan 2017
ERR1146445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4447833
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC150340.3380072947882711No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT111110.24980704086686709No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA93220.20958520699855412No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC87620.19699480623485638No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC86890.19535355756387435No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA85140.19141905732521883No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC67810.15245626353327563No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG62050.13950613703347226No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG55720.12527448759879248No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT54230.12192454168130863No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG49710.11176228963632404No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT48830.10978379808774295No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT48470.10897441518150525No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC48360.10872710373793261No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC47390.10654626646279211No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA47370.1065013007457789No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT46510.10456777491421104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46130.10371342629096011No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT45630.10258928336562996No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT45050.10128527757224698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65000.036.8908961
GTATCAA107850.036.7510571
CAACGCA119450.031.0822815
ATCAACG120850.030.8082963
GGGCACC42500.028.66287694
TATCAAC138000.028.547542
GTACTAG39150.027.5021171
AACGCAG141800.027.3422996
TAGGCAT48750.026.8968565
GTGGTAT22250.025.1466051
ACGCAGA147900.025.070687
TCAACGC151550.024.783934
CTGTGCG53600.024.7294719
CGCAGAG150100.024.7032228
TAGTACT54150.024.3942364
GTACATG161450.024.084071
CTAGGCA56100.023.8814854
ACATGGG158600.023.6827813
TACATGG164000.023.5630632
TACCCCG2853.6379788E-1223.0863425
Produced by FastQC (version 0.11.5)