FastQCFastQC Report
Mon 16 Jan 2017
ERR1146444_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146444_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4697207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143530.3055645620897695No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC112260.23899308674282396No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT92950.19788355079944317No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA71820.15289937190334596No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC67240.1431488967805762No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA64600.13752853557443817No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC58770.12511690457755004No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC54080.11513224773785782No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG47100.10027235333678076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG34150.035.4887051
GTATCAA82300.032.6604961
CAACGCA74750.031.3658585
TAGGCAT43100.030.7425335
GGGCACC43000.030.73607894
GGTATCA52800.030.1877371
GCATCGT37250.030.1231238
CTGTGCG48700.029.792329
TAGTACT50000.029.038554
ATCAACG81300.029.015643
CTAGGCA50400.027.2231754
GTACATG176700.027.1680831
TATCAAC96400.027.1531642
AACGCAG91900.026.6141996
TACATGG179850.026.207932
ACATGGG173500.026.163553
CTAGTAC56050.025.7384133
GTACTGT59450.024.0845836
ACTGTGC66400.024.0402628
TCAACGC101300.023.7954754