##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146444_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4697207
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.371648087895636	31.0	30.0	34.0	26.0	34.0
2	30.487593159083684	31.0	30.0	34.0	26.0	34.0
3	30.66428241293177	31.0	30.0	34.0	26.0	34.0
4	34.169714044963314	37.0	35.0	37.0	30.0	37.0
5	34.360146997992636	37.0	35.0	37.0	30.0	37.0
6	34.51482827986929	37.0	35.0	37.0	30.0	37.0
7	34.39168084353106	37.0	35.0	37.0	30.0	37.0
8	34.30964209156633	37.0	35.0	37.0	30.0	37.0
9	35.70307205963033	39.0	35.0	39.0	30.0	39.0
10-11	35.62966045567079	39.0	35.0	39.0	29.5	39.0
12-13	35.6292281775106	39.0	35.0	39.0	29.5	39.0
14-15	36.653639705467526	40.0	36.0	41.0	28.5	41.0
16-17	36.47774720168815	40.0	36.0	41.0	27.5	41.0
18-19	36.43000893935481	40.0	36.0	41.0	27.5	41.0
20-21	36.767193781325794	40.0	36.0	41.0	29.0	41.0
22-23	36.48754802588006	40.0	36.0	41.0	27.5	41.0
24-25	36.60879560981664	40.0	36.0	41.0	28.5	41.0
26-27	36.695079969862945	40.0	36.0	41.0	29.5	41.0
28-29	36.523200063356796	40.0	35.5	41.0	28.5	41.0
30-31	36.46107867930879	39.0	35.0	41.0	28.5	41.0
32-33	36.56672199032319	39.5	35.0	41.0	29.5	41.0
34-35	36.614618963992854	40.0	35.0	41.0	29.5	41.0
36-37	36.54397506858864	39.5	35.0	41.0	29.0	41.0
38-39	36.38213485162566	39.0	35.0	41.0	29.0	41.0
40-41	36.35221089894485	39.0	35.0	41.0	28.5	41.0
42-43	36.19126568192545	39.0	35.0	41.0	28.0	41.0
44-45	36.02908643370411	39.0	35.0	41.0	28.0	41.0
46-47	35.91279828630077	39.0	34.5	41.0	27.5	41.0
48-49	35.80126104725638	39.0	34.0	40.5	27.0	41.0
50-51	35.605375705179696	38.0	34.0	40.0	27.0	41.0
52-53	35.44457717107208	38.0	34.0	40.0	27.0	41.0
54-55	35.25740413398856	38.0	33.5	40.0	26.0	41.0
56-57	35.1383851084272	37.5	33.0	40.0	26.0	41.0
58-59	34.91466247921372	37.0	33.0	40.0	26.0	41.0
60-61	34.66942280806445	37.0	33.0	40.0	26.0	41.0
62-63	34.41135083039772	36.0	33.0	40.0	26.0	41.0
64-65	34.1314691900953	36.0	32.5	39.0	26.0	41.0
66-67	33.836521894819626	35.0	32.0	39.0	26.0	41.0
68-69	33.54726755708232	35.0	32.0	38.5	25.0	40.0
70-71	33.444341605554115	35.0	32.0	38.0	25.0	40.0
72-73	33.21288672183278	35.0	32.0	37.0	25.0	40.0
74-75	32.93754384254302	35.0	32.0	37.0	25.0	39.0
76-77	32.685824576178995	35.0	31.5	36.0	25.0	39.0
78-79	32.458933787674255	35.0	31.0	36.0	24.5	39.0
80-81	32.21326268993468	35.0	31.0	36.0	24.0	37.5
82-83	31.98722826139023	35.0	31.0	35.0	24.0	37.0
84-85	31.79574330022075	35.0	31.0	35.0	24.0	37.0
86-87	31.622426582435054	34.5	31.0	35.0	24.0	36.0
88-89	31.60388311607302	35.0	31.0	35.0	24.0	36.0
90-91	31.530851844511005	35.0	31.0	35.0	24.0	36.0
92-93	31.399781082673172	35.0	31.0	35.0	23.0	36.0
94-95	31.368753388982007	35.0	31.0	35.0	23.0	35.0
96-97	31.285527442158713	35.0	31.0	35.0	23.0	35.0
98-99	31.207444977408915	34.5	31.0	35.0	23.0	35.0
100	30.727169571194118	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7714817463640635
1101	2	0.9472370347095023
1101	3	0.8853374185492484
1101	4	0.9326748344290152
1101	5	0.8792637665536702
1101	6	0.5827719655927623
1101	7	0.5603187696667007
1101	8	0.605417805123146
1101	9	0.7633271725538506
1101	10-11	0.8149994087447681
1101	12-13	0.7274902504099217
1101	14-15	0.987232378433319
1101	16-17	1.0969863340534545
1101	18-19	1.2192932303829664
1101	20-21	0.9207010782023488
1101	22-23	1.2267131202782977
1101	24-25	1.0510826927075954
1101	26-27	0.9673669831423055
1101	28-29	1.1569539961643045
1101	30-31	1.149046314691283
1101	32-33	1.0079287295162516
1101	34-35	0.8668373385863859
1101	36-37	0.8866912927026291
1101	38-39	0.9796745519382313
1101	40-41	0.8458841225206726
1101	42-43	0.8243743933226426
1101	44-45	0.8641334063279658
1101	46-47	0.9167886228069619
1101	48-49	0.8957984532192782
1101	50-51	0.9158059307897091
1101	52-53	0.9539564065158572
1101	54-55	1.0307504771244353
1101	56-57	0.8413161657071271
1101	58-59	0.82063995553181
1101	60-61	0.8210797988084835
1101	62-63	0.7888333114888084
1101	64-65	0.7142490727724393
1101	66-67	0.7232865913937587
1101	68-69	0.774447077281728
1101	70-71	0.7461147525430221
1101	72-73	0.7520047711805447
1101	74-75	0.6982771312963507
1101	76-77	0.6772371640136896
1101	78-79	0.681842679875146
1101	80-81	0.7002006593798455
1101	82-83	0.7313963949486713
1101	84-85	0.7102035450714013
1101	86-87	0.6895828953006564
1101	88-89	0.6494816631866556
1101	90-91	0.6761714993980625
1101	92-93	0.723798920568985
1101	94-95	0.6707251667961316
1101	96-97	0.6827213621783628
1101	98-99	0.6509538418728376
1101	100	0.6833639697218921
1102	1	0.705243813253972
1102	2	0.9167602598292781
1102	3	0.8679949578956645
1102	4	0.9579857348872522
1102	5	0.8441502268360637
1102	6	0.6464068632570275
1102	7	0.7026474814789765
1102	8	0.7027988952121476
1102	9	0.8847046521833875
1102	10-11	0.8456004433171103
1102	12-13	0.7773698297946936
1102	14-15	1.1033965447408747
1102	16-17	1.212587808667223
1102	18-19	1.2163040301223447
1102	20-21	0.9827400700340689
1102	22-23	1.2520846294759522
1102	24-25	1.0657210182985253
1102	26-27	0.9078432539964822
1102	28-29	1.0558891979635376
1102	30-31	1.2125463147305382
1102	32-33	1.100685557283093
1102	34-35	0.9247304610410794
1102	36-37	0.8917170094147124
1102	38-39	0.9486192854823514
1102	40-41	0.9663062882986111
1102	42-43	1.0037570077371498
1102	44-45	1.0555326258098745
1102	46-47	1.0266387245189534
1102	48-49	0.928864033838046
1102	50-51	1.041626926018182
1102	52-53	0.9744164488304818
1102	54-55	1.0364030467897507
1102	56-57	0.8794687194744242
1102	58-59	0.8894765864804057
1102	60-61	0.8828740396750021
1102	62-63	0.8026824869548008
1102	64-65	0.7928710121782956
1102	66-67	0.8694719464224008
1102	68-69	0.9164770633770445
1102	70-71	0.9159722756852418
1102	72-73	0.817329431863854
1102	74-75	0.8411584856849856
1102	76-77	0.7717191033551813
1102	78-79	0.7923110378344376
1102	80-81	0.9164970579410365
1102	82-83	0.8330523835121504
1102	84-85	0.8332217982725751
1102	86-87	0.8290444683000011
1102	88-89	0.7631017344772957
1102	90-91	0.7673821958670608
1102	92-93	0.8462295751818409
1102	94-95	0.7949587547620638
1102	96-97	0.7565932682769017
1102	98-99	0.7774402378408816
1102	100	0.8129932271619893
1103	1	0.6526696597172119
1103	2	0.8358239595589652
1103	3	0.751863590428556
1103	4	0.7822692581090038
1103	5	0.7407056651863613
1103	6	0.5365931639250974
1103	7	0.5755513105905337
1103	8	0.5688289146067049
1103	9	0.6707309485875186
1103	10-11	0.810318546119376
1103	12-13	0.6917930765287181
1103	14-15	0.867063678372368
1103	16-17	1.0379941754581452
1103	18-19	1.164168163246302
1103	20-21	0.8867899954947092
1103	22-23	1.0234129693881613
1103	24-25	0.9731114327119101
1103	26-27	0.854211130794134
1103	28-29	0.9927283870516774
1103	30-31	1.0323459812524192
1103	32-33	0.8413843657670554
1103	34-35	0.8872327282467865
1103	36-37	0.8525421012564252
1103	38-39	0.9685200415357826
1103	40-41	0.8391385811038532
1103	42-43	0.8508470094395406
1103	44-45	0.9142703769327412
1103	46-47	0.9167015420271838
1103	48-49	0.913342365715593
1103	50-51	0.8944872446679781
1103	52-53	0.9197694925342574
1103	54-55	0.8975516927508593
1103	56-57	0.7932919720952256
1103	58-59	0.8421353823634803
1103	60-61	0.8018238620374376
1103	62-63	0.7118197038126226
1103	64-65	0.7691671975450447
1103	66-67	0.8222048839095777
1103	68-69	0.8801154591818374
1103	70-71	0.80725890696759
1103	72-73	0.7916449147309379
1103	74-75	0.7128347223462583
1103	76-77	0.7030297113322419
1103	78-79	0.7505737496988445
1103	80-81	0.7568663061992638
1103	82-83	0.6433575494000046
1103	84-85	0.6234421468017572
1103	86-87	0.6904046008002744
1103	88-89	0.7392285953758702
1103	90-91	0.7416791234453548
1103	92-93	0.7730578578172747
1103	94-95	0.7070694317894635
1103	96-97	0.7026464211247472
1103	98-99	0.7279367360024551
1103	100	0.7958893477741498
1104	1	0.5240516996584823
1104	2	0.6898531601413964
1104	3	0.6431618715236382
1104	4	0.7025112955905755
1104	5	0.6538406131151291
1104	6	0.3153957532903888
1104	7	0.4069785664919152
1104	8	0.4311498841534913
1104	9	0.5598011517556714
1104	10-11	0.5542905738684283
1104	12-13	0.4689994043338075
1104	14-15	0.5445320894519412
1104	16-17	0.7631594649966758
1104	18-19	0.7396571044670708
1104	20-21	0.5858466361523256
1104	22-23	0.8808710026609106
1104	24-25	0.7000800275097916
1104	26-27	0.596796901431695
1104	28-29	0.7883334579806132
1104	30-31	0.7928475701880231
1104	32-33	0.6065480344849945
1104	34-35	0.4892692115139994
1104	36-37	0.5102997396107369
1104	38-39	0.622185164816841
1104	40-41	0.5199826262772973
1104	42-43	0.5005378578385375
1104	44-45	0.5579787271575256
1104	46-47	0.5324765106356466
1104	48-49	0.48685650595187724
1104	50-51	0.6107960212407093
1104	52-53	0.6286586686411582
1104	54-55	0.6050122882107374
1104	56-57	0.4778029056119806
1104	58-59	0.46110167840503635
1104	60-61	0.5120059177693719
1104	62-63	0.48005349902289396
1104	64-65	0.43176831853037356
1104	66-67	0.6009668578640799
1104	68-69	0.5024794431057273
1104	70-71	0.38349579805907297
1104	72-73	0.42394037248559613
1104	74-75	0.40112503371083363
1104	76-77	0.44145661573951145
1104	78-79	0.451542427245883
1104	80-81	0.4664173237101963
1104	82-83	0.3675091807730233
1104	84-85	0.3993121516756091
1104	86-87	0.39489241389953733
1104	88-89	0.339989792702994
1104	90-91	0.4416903970687187
1104	92-93	0.4364421160432208
1104	94-95	0.333744627692397
1104	96-97	0.40469525982766896
1104	98-99	0.44097050600839083
1104	100	0.4949363659956667
1105	1	0.527055084675407
1105	2	0.6323867822235059
1105	3	0.6685446128710311
1105	4	0.7635005790660259
1105	5	0.6186529370831977
1105	6	0.3560291142113172
1105	7	0.4565926023146858
1105	8	0.4191520557282544
1105	9	0.45412582890547526
1105	10-11	0.5276880427801984
1105	12-13	0.5141983575867997
1105	14-15	0.6838051681984396
1105	16-17	0.7954779989497922
1105	18-19	0.7825601685186498
1105	20-21	0.6319614264115216
1105	22-23	0.8863934599277457
1105	24-25	0.735926491414979
1105	26-27	0.5115464361986781
1105	28-29	0.7641023670718639
1105	30-31	0.7732668478588636
1105	32-33	0.6557245408530576
1105	34-35	0.569566808158271
1105	36-37	0.5420154757663269
1105	38-39	0.6160944698141009
1105	40-41	0.5388591006039718
1105	42-43	0.5461368657806318
1105	44-45	0.5702046519894068
1105	46-47	0.5368018943467163
1105	48-49	0.5533087774797067
1105	50-51	0.5874200304587944
1105	52-53	0.5822163489305865
1105	54-55	0.5144379171973767
1105	56-57	0.5289434228066767
1105	58-59	0.5057386859815267
1105	60-61	0.5123440974566051
1105	62-63	0.47333298737926555
1105	64-65	0.48949342963241804
1105	66-67	0.5695913740741148
1105	68-69	0.5198780792315318
1105	70-71	0.46576702867966446
1105	72-73	0.44200593914975883
1105	74-75	0.4688088648318143
1105	76-77	0.44944019353847153
1105	78-79	0.422010803706911
1105	80-81	0.45935072103994656
1105	82-83	0.459439111215044
1105	84-85	0.4820376967446762
1105	86-87	0.5005408289427287
1105	88-89	0.4846744589283034
1105	90-91	0.5574058758420684
1105	92-93	0.5874020011664989
1105	94-95	0.5597217391789187
1105	96-97	0.5557071192122791
1105	98-99	0.5695492767257839
1105	100	0.6249974533797484
1106	1	0.5901219706810004
1106	2	0.6299390108789709
1106	3	0.6398659902265642
1106	4	0.8366780762084858
1106	5	0.6387276849545813
1106	6	0.40458776901238735
1106	7	0.48382823940502107
1106	8	0.49782234295906136
1106	9	0.6140073446283125
1106	10-11	0.6087398566036626
1106	12-13	0.5529480835590235
1106	14-15	0.7672173430445994
1106	16-17	0.9040903399508622
1106	18-19	0.8580953539306435
1106	20-21	0.6744927969577503
1106	22-23	0.9914866508311917
1106	24-25	0.825066075139695
1106	26-27	0.7136616963449924
1106	28-29	0.8929398171320813
1106	30-31	0.9223345266180942
1106	32-33	0.7993808355622605
1106	34-35	0.8085722544951608
1106	36-37	0.6889392018093545
1106	38-39	0.8276964602773731
1106	40-41	0.7300208253782685
1106	42-43	0.7699900603333276
1106	44-45	0.7735578674065167
1106	46-47	0.742197629615184
1106	48-49	0.7726654130421124
1106	50-51	0.8065891121555779
1106	52-53	0.670818332824922
1106	54-55	0.7468906480048148
1106	56-57	0.7475700579551798
1106	58-59	0.7326921424508583
1106	60-61	0.7046066200910133
1106	62-63	0.6534616717859763
1106	64-65	0.716995073422666
1106	66-67	0.6585243558613527
1106	68-69	0.6862854417856212
1106	70-71	0.7269851713206208
1106	72-73	0.6536848183220627
1106	74-75	0.660166105264608
1106	76-77	0.6627977405097027
1106	78-79	0.6522604706620996
1106	80-81	0.7361631549587102
1106	82-83	0.6147902820889151
1106	84-85	0.588726323654825
1106	86-87	0.5846081388808493
1106	88-89	0.6249346537076335
1106	90-91	0.559135627106432
1106	92-93	0.6528846137119437
1106	94-95	0.6243170823733166
1106	96-97	0.5868074068024001
1106	98-99	0.6109427320228704
1106	100	0.6254382506078109
1107	1	0.44518226117608606
1107	2	0.6076400924719962
1107	3	0.5388281236035155
1107	4	0.6665688301923964
1107	5	0.5429815419585609
1107	6	0.3360476083187791
1107	7	0.3419724201280516
1107	8	0.42636770882602093
1107	9	0.5185502489971583
1107	10-11	0.5425379257164309
1107	12-13	0.4375078190934758
1107	14-15	0.6369806457989213
1107	16-17	0.7793979627485612
1107	18-19	0.8903837662127927
1107	20-21	0.6313913553862776
1107	22-23	0.8522777340340184
1107	24-25	0.5800369051714398
1107	26-27	0.5343901019668706
1107	28-29	0.7087425189596388
1107	30-31	0.7239052880579706
1107	32-33	0.6485664359088332
1107	34-35	0.5275355058976601
1107	36-37	0.5255626062284833
1107	38-39	0.5336023888335149
1107	40-41	0.4098523718967897
1107	42-43	0.5453931665656455
1107	44-45	0.5151777961844246
1107	46-47	0.4556265347937156
1107	48-49	0.470533382512464
1107	50-51	0.5241751015824079
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2214	100	-1.1042425710577817
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2215	8	-1.2705531209017522
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2215	10-11	-1.4761021234171352
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2215	14-15	-3.767261492942417
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2215	24-25	-5.736560926293777
2215	26-27	-3.7601946425257466
2215	28-29	-1.9356777817010737
2215	30-31	-4.098695854124379
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2216	3	-0.8800733267597067
2216	4	-0.871140316871795
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2216	6	-0.9483306877084061
2216	7	-1.042301812300927
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2216	12-13	-1.3432670430574802
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2216	86-87	-1.5322549796219143
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2216	90-91	-1.5631369731191072
2216	92-93	-1.4444390094967652
2216	94-95	-1.3092304463233972
2216	96-97	-1.3591153765861357
2216	98-99	-1.2206117732121484
2216	100	-1.2179403336091426
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	19214.0
1	3304.0
2	482.0
3	505.0
4	516.0
5	276.0
6	248.0
7	302.0
8	522.0
9	916.0
10	1776.0
11	3462.0
12	5993.0
13	8545.0
14	11349.0
15	14143.0
16	17028.0
17	20045.0
18	22904.0
19	26535.0
20	30156.0
21	34300.0
22	39031.0
23	44981.0
24	49839.0
25	56450.0
26	63179.0
27	72001.0
28	84649.0
29	99936.0
30	119368.0
31	141369.0
32	174023.0
33	220255.0
34	295211.0
35	399516.0
36	589686.0
37	909160.0
38	850291.0
39	265428.0
40	313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.48582561517669	19.80416275114025	12.513348629870395	24.196663003812667
2	15.578277058728428	19.631280371649336	40.61439585170637	24.17604671791586
3	18.845884306911927	25.769427575661375	28.812589792933963	26.572098324492732
4	12.249701731773769	16.343259351976187	39.14410601023844	32.26293290601161
5	13.336908384607096	38.0725855147133	35.03216690870961	13.55833919196999
6	30.955403547640532	37.555339623876684	17.987821966755362	13.501434861727422
7	27.61010654823599	31.60168556587649	22.99208912085081	17.796118765036702
8	26.03340790359146	35.35086001868001	19.965015101279675	18.65071697644885
9	26.16740787631825	15.402351540318616	20.790465263943183	37.63977531941995
10-11	25.433737202616168	25.833921488153877	27.919569315494808	20.81277199373515
12-13	25.690818516001645	23.604659500125745	27.802158523599825	22.902363460272785
14-15	23.04833577019928	25.017729390272702	25.03246508992897	26.90146974959905
16-17	22.476681801895595	27.742491495262733	26.170040808184407	23.610785894657266
18-19	22.156906244318073	27.94093395142011	27.555159450356946	22.34700035390487
20-21	23.518550999557707	27.09170748130207	26.82924245247169	22.560499066668534
22-23	23.67465340800353	26.877526422635288	26.554514459906777	22.893305709454403
24-25	23.214421965116884	26.974568830136224	27.114670610462582	22.696338594284313
26-27	23.092561998856684	27.113691612788227	26.4380913374767	23.355655050878386
28-29	22.551114479914368	27.433252969970525	26.881362961491924	23.134269588623184
30-31	23.105298181116645	27.225212849703055	26.760013905891693	22.909475063288607
32-33	23.270011833524283	27.378809634558216	26.137375671219985	23.213802860697516
34-35	23.417367888050574	27.21892971869134	26.749562914939183	22.6141394783189
36-37	23.19250871546327	26.89887921871772	26.516501312805126	23.39211075301389
38-39	23.202420475147747	27.104366401887063	26.875900346974728	22.817312775990462
40-41	23.0052918932451	26.51644909393065	27.33382788412356	23.14443112870069
42-43	22.95538941946267	26.611146929835826	26.884025676460162	23.549437974241343
44-45	23.0902280121228	27.182218098608086	26.805580323167565	22.92197356610155
46-47	23.088446015223436	27.04061377550436	26.44899919925665	23.421941010015555
48-49	23.37582569038042	26.86978676776659	26.590242487862763	23.164145053990225
50-51	23.155739525180994	26.8096867948877	26.663772059650594	23.370801620280716
52-53	22.945716474623445	27.102787936147756	26.52902864580073	23.422466943428073
54-55	22.81276669432921	27.038371447634212	26.59940609373599	23.549455764300596
56-57	23.503534343135467	26.758458105018494	26.366611787279147	23.371395764566895
58-59	22.81507172276544	26.91701802641946	26.944083019002	23.32382723181311
60-61	22.836542217232157	27.370097078734602	26.500189517448554	23.29317118658469
62-63	23.046851592822325	26.926365245707416	26.31957629744782	23.707206864022435
64-65	22.569162520002926	26.958274594058235	26.574766388645692	23.89779649729315
66-67	22.964215115109372	26.847117835253748	26.472908567431723	23.715758482205153
68-69	22.705962076880006	26.84728006847822	26.688511563191696	23.75824629145008
70-71	22.63855589436778	26.617930645078953	26.828769998890206	23.914743461663058
72-73	22.982397014287383	26.706222021920865	27.015018356220594	23.296362607571155
74-75	23.153547762369346	26.891668680448284	26.697992475555832	23.25679108162654
76-77	22.9883898836677	26.824164810618928	26.472708875193334	23.714736430520038
78-79	22.861348643594877	26.82078831062944	26.517188453164987	23.800674592610694
80-81	22.639475586221124	27.10643337033834	26.850041553045894	23.404049490394645
82-83	23.194709909353058	26.485424866264008	27.486568079054724	22.83329714532821
84-85	23.293945831831003	26.81058842377968	26.650760978344312	23.244704766045004
86-87	22.45923726338933	27.23601377580817	26.97539850218146	23.329350458621047
88-89	23.16896420321088	26.40968560743881	26.81418910918161	23.607161080168705
90-91	22.931605010640382	26.557235745702435	26.866162591683224	23.64499665197396
92-93	23.144553775948143	26.63991963404342	26.87470783330199	23.340818756706447
94-95	22.467135424033387	26.574908714594276	27.30397561101104	23.653980250361297
96-97	22.765640361400784	26.439374353253747	27.007660747725314	23.787324537620155
98-99	22.313738173563998	26.975031629544443	26.86160116799257	23.849629028898992
100	22.92252952685992	26.505982861829146	26.326959793532613	24.244527817778323
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16294.0
1	9695.5
2	2940.0
3	2213.0
4	1281.0
5	779.0
6	594.5
7	564.0
8	586.5
9	580.0
10	534.0
11	495.5
12	487.0
13	493.0
14	523.5
15	608.0
16	730.0
17	858.5
18	1033.0
19	1303.0
20	1741.0
21	2283.5
22	3063.5
23	4361.5
24	6029.5
25	8310.0
26	11793.0
27	16135.0
28	21503.0
29	29624.0
30	40605.5
31	51432.0
32	65305.5
33	78742.0
34	88652.0
35	100266.0
36	113807.5
37	132476.5
38	141193.5
39	138164.5
40	134844.5
41	131013.5
42	132134.0
43	139729.5
44	149976.5
45	169108.0
46	187753.5
47	198636.0
48	206721.5
49	205347.0
50	198686.0
51	199460.0
52	199470.0
53	193319.5
54	184283.0
55	164464.5
56	146639.5
57	126319.0
58	106228.0
59	98641.0
60	83893.5
61	63806.0
62	49725.5
63	39379.0
64	28864.5
65	18305.5
66	12479.5
67	9403.5
68	7229.0
69	5536.0
70	4181.5
71	3067.5
72	2219.0
73	1847.0
74	1517.0
75	1068.0
76	707.0
77	430.0
78	245.0
79	144.5
80	97.0
81	63.5
82	48.0
83	38.0
84	32.0
85	25.0
86	17.5
87	11.0
88	13.0
89	16.0
90	15.5
91	10.0
92	5.0
93	7.0
94	7.5
95	6.5
96	7.0
97	6.0
98	3.5
99	4.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9988276011680983
2	0.4464567986890934
3	0.4803492799018651
4	0.465915170440647
5	0.43325746555346617
6	0.48728957442156584
7	0.503341666654248
8	0.5058750870464086
9	0.5203091965076267
10-11	0.5345623473694048
12-13	0.5329124307274514
14-15	0.5296871098080199
16-17	0.5344665457579366
18-19	0.5332424140558422
20-21	0.5314966958024204
22-23	0.4945811415166502
24-25	0.5298787130309565
26-27	0.5099945563395438
28-29	0.516583578283861
30-31	0.5187657261006381
32-33	0.520852072305947
34-35	0.5335404635137434
36-37	0.5516469680812448
38-39	0.540683005879877
40-41	0.5455901773117514
42-43	0.5436209219649039
44-45	0.5536907357925678
46-47	0.5518705051746708
48-49	0.5237580545204842
50-51	0.5386285935450578
52-53	0.5311986463445192
54-55	0.5413003940426726
56-57	0.5288142506813092
58-59	0.5347858844628308
60-61	0.528271374882989
62-63	0.5214801050922389
64-65	0.5338810914656305
66-67	0.5312731587089945
68-69	0.5522537116205438
70-71	0.5357758344480028
72-73	0.5398420806236557
74-75	0.5372128586200268
76-77	0.524652202894188
78-79	0.5554683879164789
80-81	0.5293784157266221
82-83	0.5290058539042457
84-85	0.5384795688161071
86-87	0.8466094851685267
88-89	1.1150668897495895
90-91	1.1056038194612245
92-93	1.1061147613890552
94-95	1.11044712315212
96-97	1.103591985620391
98-99	1.0991531776223615
100	1.0950549975762192
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4697207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.81433306443865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1576731218796	31.426679762664822
2	12.874209859600363	10.766529980216191
3	4.747695679265875	5.955651852642588
4	2.329357485308539	3.8960211886733807
5	1.266574266548081	2.648047911614428
6	0.7943286174764509	1.992859282426524
7	0.510580486401383	1.4944707760213363
8	0.36220316734793756	1.2116227101185018
9	0.2500004772407628	0.9408242899452484
>10	1.4177806330329807	10.854697861678279
>50	0.13138183911026952	3.830029689379275
>100	0.12840492580029328	11.246448424630433
>500	0.02044948019701492	5.8572010297162835
>1k	0.008953005973578338	6.463883231700765
>5k	3.0521611273562436E-4	0.8709492765574675
>10k+	1.0173870424520812E-4	0.5440827320144449
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14353	0.3055645620897695	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	11226	0.23899308674282396	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9295	0.19788355079944317	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7182	0.15289937190334596	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6724	0.1431488967805762	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	6460	0.13752853557443817	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5877	0.12511690457755004	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5408	0.11513224773785782	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	4710	0.10027235333678076	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.257849398589417E-5	0.0
3	0.0	0.0	0.0	4.257849398589417E-5	0.0
4	0.0	0.0	0.0	4.257849398589417E-5	0.0
5	0.0	0.0	0.0	4.257849398589417E-5	0.0
6	0.0	0.0	0.0	4.257849398589417E-5	0.0
7	0.0	0.0	0.0	4.257849398589417E-5	0.0
8	0.0	0.0	0.0	4.257849398589417E-5	0.0
9	0.0	0.0	0.0	6.386774097884125E-5	0.0
10-11	0.0	0.0	0.0	1.3838010545415605E-4	4.257849398589417E-5
12-13	0.0	0.0	0.0	1.703139759435767E-4	1.3838010545415605E-4
14-15	0.0	0.0	0.0	1.9160322293652378E-4	1.8095859944005022E-4
16-17	0.0	0.0	0.0	1.9160322293652378E-4	2.1289246992947086E-4
18-19	0.0	0.0	0.0	2.1289246992947086E-4	2.1289246992947086E-4
20-21	0.0	0.0	0.0	2.767602109083121E-4	3.4062795188715333E-4
22-23	0.0	0.0	0.0	3.8320644587304755E-4	3.6191719888010044E-4
24-25	0.0	0.0	0.0	5.322311748236771E-4	3.72561822376574E-4
26-27	0.0	0.0	0.0	7.344790212566745E-4	4.1514031636246816E-4
28-29	0.0	0.0	0.0	0.0016605612654498727	4.257849398589417E-4
30-31	0.0	0.0	0.0	0.006376129474387652	4.364295633554153E-4
32-33	0.0	0.0	0.0	0.01706333146484709	4.470741868518888E-4
34-35	0.0	0.0	0.0	0.033285737673472766	4.89652680837783E-4
36-37	0.0	0.0	0.0	0.061291742092694654	4.89652680837783E-4
38-39	0.0	1.0644623496473543E-5	0.0	0.10837291181759715	4.89652680837783E-4
40-41	0.0	2.1289246992947087E-5	0.0	0.16257533466164042	4.89652680837783E-4
42-43	0.0	2.1289246992947087E-5	0.0	0.21849154188861591	4.89652680837783E-4
44-45	0.0	2.1289246992947087E-5	0.0	0.29047048597177005	4.89652680837783E-4
46-47	0.0	2.1289246992947087E-5	0.0	0.3815245953606047	4.89652680837783E-4
48-49	0.0	2.1289246992947087E-5	0.0	0.48667218625877035	4.89652680837783E-4
50-51	0.0	2.1289246992947087E-5	0.0	0.6019960372195647	4.89652680837783E-4
52-53	0.0	2.1289246992947087E-5	0.0	0.7182672596715451	5.535204218166242E-4
54-55	0.0	2.1289246992947087E-5	0.0	0.8519850200342459	5.960989158025184E-4
56-57	0.0	2.1289246992947087E-5	0.0	1.0152628998466535	6.067435392989919E-4
58-59	0.0	2.1289246992947087E-5	0.0	1.2100062867146368	6.173881627954654E-4
60-61	0.0	2.1289246992947087E-5	0.0	1.418119320694191	6.386774097884126E-4
62-63	0.0	2.1289246992947087E-5	0.0	1.639506200173848	7.45123644753148E-4
64-65	0.0	2.1289246992947087E-5	0.0	1.8661515236607626	8.409252562214099E-4
66-67	0.0	2.1289246992947087E-5	0.0	2.1330441685878436	8.728591267108305E-4
68-69	0.0	2.1289246992947087E-5	0.0	2.436650119954262	8.728591267108305E-4
70-71	0.0	2.1289246992947087E-5	0.0	2.7736056767351323	8.728591267108305E-4
72-73	0.0	2.1289246992947087E-5	0.0	3.1263472101612724	8.83503750207304E-4
74-75	0.0	2.1289246992947087E-5	0.0	3.4737770764626723	9.154376206967247E-4
76-77	0.0	2.1289246992947087E-5	0.0	3.8645305603947198	9.79305361675566E-4
78-79	0.0	2.1289246992947087E-5	0.0	4.2975857781017535	9.79305361675566E-4
80-81	0.0	2.1289246992947087E-5	0.0	4.778477933801938	0.0010112392321649866
82-83	0.0	2.1289246992947087E-5	0.0	5.274134182291732	0.0011709085846120897
84-85	0.0	2.1289246992947087E-5	0.0	5.75818779117037	0.0011709085846120897
86-87	0.0	3.193387048942063E-5	0.0	6.27133315606487	0.0012347763255909309
88	0.0	4.257849398589417E-5	0.0	6.686484117050835	0.001298644066569772
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3415	0.0	35.488705	1
GTATCAA	8230	0.0	32.660496	1
CAACGCA	7475	0.0	31.365858	5
TAGGCAT	4310	0.0	30.742533	5
GGGCACC	4300	0.0	30.736078	94
GGTATCA	5280	0.0	30.187737	1
GCATCGT	3725	0.0	30.123123	8
CTGTGCG	4870	0.0	29.79232	9
TAGTACT	5000	0.0	29.03855	4
ATCAACG	8130	0.0	29.01564	3
CTAGGCA	5040	0.0	27.223175	4
GTACATG	17670	0.0	27.168083	1
TATCAAC	9640	0.0	27.153164	2
AACGCAG	9190	0.0	26.614199	6
TACATGG	17985	0.0	26.20793	2
ACATGGG	17350	0.0	26.16355	3
CTAGTAC	5605	0.0	25.738413	3
GTACTGT	5945	0.0	24.084583	6
ACTGTGC	6640	0.0	24.040262	8
TCAACGC	10130	0.0	23.795475	4
>>END_MODULE