FastQCFastQC Report
Mon 16 Jan 2017
ERR1146444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4697207
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC127060.2705011722923857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94830.20188592923411722No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT94350.20086404537845576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87050.1853228950736044No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA79830.16995205874469657No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC71870.1530058181383107No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA71680.15260132244544472No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC67900.1445539870821107No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC58720.12501045834258528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53730.11438712409310468No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG51760.11019314243549412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84650.044.9925581
GTATCAA142800.038.1625941
GTACTAG34850.033.3255121
CAACGCA170200.031.0916775
ATCAACG172750.030.6680933
GGGCACC44400.030.19622894
AACGCAG188450.028.6542596
TATCAAC189100.028.5412432
CTGTGCG50750.028.155789
TAGGCAT45900.028.054545
TCAACGC193900.027.5165714
TAGTACT53600.027.0123394
ACGCAGA206100.025.6302977
GTGGTAT30850.025.6057051
CTAGTAC56950.025.588743
CTAGGCA52000.025.4930634
CGCAGAG210300.025.1184228
GCATCGT44350.025.008278
GTACATG168350.024.6341781
GTACTGT60800.024.1939096
Produced by FastQC (version 0.11.5)