FastQCFastQC Report
Mon 16 Jan 2017
ERR1146443_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146443_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4662931
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143950.3087114091973482No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC136660.2930774656541133No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT112790.24188648727592155No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA111390.23888408385198068No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC97660.20943908455861773No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA95350.20448511890911533No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC87940.18859382650097117No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC77120.16538953718165678No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT60880.13056165746394274No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG59640.12790238585988084No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT58220.1248570909584551No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC56320.12078240059739251No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC53710.11518506278561703No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT52320.11220410510041859No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA49760.1067139959823553No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT49570.10630652694624905No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC47980.1028966544862019No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG47980.1028966544862019No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC42500.033.33489294
GTACTAG43750.031.0575031
TAGGCAT52400.029.8576535
GCATCGT43650.029.2533638
GTATCAA111150.028.3118651
CTAGGCA60150.027.8071884
TACCCCG3450.027.2366475
CAACGCA103300.026.152325
GGTATCA76200.026.1282041
TAGTACT60650.025.9511574
ATCAACG107650.025.184733
CTGTGCG60100.025.15399
GTACATG181000.024.8147811
TCTAACG19200.024.4733312
TACATGG184500.024.2711772
AGGGCTA51750.024.2406145
CTATTGA49400.024.2346739
ACATGGG178850.024.2224183
CTAACGC19600.024.2125683
GCTATTG50700.023.7040828
Produced by FastQC (version 0.11.5)