##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146443_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4662931 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.454704991345572 31.0 30.0 34.0 27.0 34.0 2 30.56533648042401 31.0 31.0 34.0 26.0 34.0 3 30.777972052342186 31.0 31.0 34.0 27.0 34.0 4 34.21952565886135 37.0 35.0 37.0 30.0 37.0 5 34.46361247893224 37.0 35.0 37.0 30.0 37.0 6 34.58535586308268 37.0 35.0 37.0 30.0 37.0 7 34.48856180801303 37.0 35.0 37.0 30.0 37.0 8 34.40568196269685 37.0 35.0 37.0 30.0 37.0 9 35.799935062303085 39.0 35.0 39.0 30.0 39.0 10-11 35.72279977979515 39.0 35.0 39.0 29.5 39.0 12-13 35.70352317029783 39.0 35.0 39.0 30.0 39.0 14-15 36.72532490830338 40.0 36.0 41.0 29.5 41.0 16-17 36.558532605350585 40.0 36.0 41.0 28.0 41.0 18-19 36.51667631367481 40.0 36.0 41.0 27.5 41.0 20-21 36.85287976596695 40.0 36.0 41.0 30.0 41.0 22-23 36.57629364449099 40.0 36.0 41.0 28.0 41.0 24-25 36.69381243256656 40.0 36.0 41.0 29.5 41.0 26-27 36.77268717894388 40.0 36.0 41.0 29.5 41.0 28-29 36.614457194412694 40.0 35.5 41.0 29.5 41.0 30-31 36.563404541049394 40.0 35.0 41.0 29.0 41.0 32-33 36.66296638316115 40.0 35.5 41.0 30.0 41.0 34-35 36.71065784589135 40.0 35.0 41.0 30.0 41.0 36-37 36.64471938358084 40.0 35.0 41.0 30.0 41.0 38-39 36.47871950067457 39.0 35.0 41.0 29.0 41.0 40-41 36.449007609162564 39.0 35.0 41.0 29.0 41.0 42-43 36.29384243515506 39.0 35.0 41.0 28.0 41.0 44-45 36.125107255500886 39.0 35.0 41.0 28.0 41.0 46-47 36.00520391573454 39.0 35.0 41.0 28.0 41.0 48-49 35.889071701897365 39.0 34.0 41.0 28.0 41.0 50-51 35.684397538801235 38.0 34.0 40.0 27.0 41.0 52-53 35.51225066379923 38.0 34.0 40.0 27.0 41.0 54-55 35.326639403413864 38.0 34.0 40.0 27.0 41.0 56-57 35.19448904562388 37.5 33.5 40.0 27.0 41.0 58-59 34.954412471469126 37.0 33.0 40.0 26.5 41.0 60-61 34.70664706383174 36.5 33.0 40.0 26.0 41.0 62-63 34.452664643761615 36.0 33.0 39.5 26.0 41.0 64-65 34.170280988502725 35.5 33.0 39.0 26.0 41.0 66-67 33.88114171108258 35.0 32.0 39.0 26.0 41.0 68-69 33.59309927597042 35.0 32.0 38.5 25.0 40.5 70-71 33.508610035190316 35.0 32.0 38.0 26.0 40.0 72-73 33.29419768381733 35.0 32.0 37.0 25.5 40.0 74-75 33.02114517671396 35.0 32.0 37.0 25.0 39.5 76-77 32.77666793696926 35.0 32.0 36.0 25.0 39.0 78-79 32.55603932376439 35.0 32.0 36.0 25.0 39.0 80-81 32.32202889127032 35.0 31.5 36.0 24.5 38.0 82-83 32.09038081412743 35.0 31.0 35.0 24.0 37.0 84-85 31.908139858814124 35.0 31.0 35.0 24.0 37.0 86-87 31.736457820199355 35.0 31.0 35.0 24.0 36.0 88-89 31.719061144160186 35.0 31.5 35.0 24.0 36.0 90-91 31.64366811346769 35.0 31.5 35.0 24.0 36.0 92-93 31.50570628216459 35.0 31.0 35.0 24.0 36.0 94-95 31.480619593127155 35.0 31.0 35.0 24.0 35.0 96-97 31.397385142520875 35.0 31.0 35.0 23.5 35.0 98-99 31.320418745205536 35.0 31.0 35.0 23.0 35.0 100 30.850361071180338 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9013098102485877 1101 2 1.067961338063693 1101 3 0.9766689414516563 1101 4 0.9968219846508575 1101 5 0.9385125743020879 1101 6 0.7556147520241367 1101 7 0.7720165259346032 1101 8 0.8728714101732606 1101 9 0.9558672636362004 1101 10-11 1.0358609696752268 1101 12-13 0.92281621000798 1101 14-15 1.1518543762973081 1101 16-17 1.3159774457052933 1101 18-19 1.4744896115092843 1101 20-21 1.1533098887969544 1101 22-23 1.436798155961526 1101 24-25 1.2789907130242568 1101 26-27 1.1335567354207043 1101 28-29 1.3870816720499235 1101 30-31 1.3487058089370692 1101 32-33 1.2656805291941637 1101 34-35 1.0877266650929016 1101 36-37 1.0312624626378977 1101 38-39 1.2042408707308923 1101 40-41 1.200472855960804 1101 42-43 1.1626016252989473 1101 44-45 1.149927975176972 1101 46-47 1.1148530257935008 1101 48-49 1.0997675276578747 1101 50-51 1.1457353874296672 1101 52-53 1.1345793499707355 1101 54-55 1.2224814609248043 1101 56-57 1.004430182972392 1101 58-59 0.9159321553018103 1101 60-61 0.9882834709458663 1101 62-63 0.9764574766730973 1101 64-65 0.913519851115602 1101 66-67 1.0124667960168665 1101 68-69 1.0216558852836215 1101 70-71 0.8668606644248413 1101 72-73 0.8001806242268472 1101 74-75 0.8470104445914757 1101 76-77 0.8690728870487234 1101 78-79 0.8339259797670167 1101 80-81 0.9164738142399784 1101 82-83 0.8923684265306804 1101 84-85 0.8684999769514299 1101 86-87 0.8556004770523558 1101 88-89 0.8595796006714167 1101 90-91 0.8754368782817323 1101 92-93 0.9669213265248864 1101 94-95 0.9528720345878838 1101 96-97 0.8627211460567601 1101 98-99 0.8690621267455043 1101 100 0.9963686183886544 1102 1 0.8065770184171015 1102 2 0.9984741928670573 1102 3 0.9554906196694226 1102 4 1.0464669662175865 1102 5 0.8749903957191023 1102 6 0.7152381798778507 1102 7 0.7768563355486862 1102 8 0.7863072487738023 1102 9 0.9211259003716776 1102 10-11 0.8961609620109954 1102 12-13 0.8696436262370781 1102 14-15 1.0730362173723051 1102 16-17 1.250494571365948 1102 18-19 1.294707303228627 1102 20-21 1.0547573543587134 1102 22-23 1.3228049982487278 1102 24-25 1.1817682952857709 1102 26-27 1.045767136729566 1102 28-29 1.2201658981105297 1102 30-31 1.21698571940626 1102 32-33 1.1261389874623902 1102 34-35 1.0130207606581223 1102 36-37 0.8949980863269573 1102 38-39 0.9890901198702267 1102 40-41 0.955191680110353 1102 42-43 0.9832326384016667 1102 44-45 0.9866990123360111 1102 46-47 0.9502000202392793 1102 48-49 0.9134522389116313 1102 50-51 0.9703380006218509 1102 52-53 0.8978688837504691 1102 54-55 0.9368273495305814 1102 56-57 0.8558336769262453 1102 58-59 0.8389109863648159 1102 60-61 0.8762974636538914 1102 62-63 0.8603084393169738 1102 64-65 0.8202556406024542 1102 66-67 0.8722031931682679 1102 68-69 0.8747331744271563 1102 70-71 0.8472030681487155 1102 72-73 0.7703530734825463 1102 74-75 0.8147777114148624 1102 76-77 0.805935445371226 1102 78-79 0.753539408918968 1102 80-81 0.8123263668221199 1102 82-83 0.791208213464877 1102 84-85 0.7556632197372757 1102 86-87 0.7187009075009669 1102 88-89 0.746617830357664 1102 90-91 0.7397615924760572 1102 92-93 0.7928952321421541 1102 94-95 0.8051283704879566 1102 96-97 0.8847810311244793 1102 98-99 0.8234871095009737 1102 100 0.8843431168112303 1103 1 0.6729009717705239 1103 2 0.8470169946411445 1103 3 0.7592967161249788 1103 4 0.7469418683995599 1103 5 0.7797519775103083 1103 6 0.608384879764678 1103 7 0.6734071934816868 1103 8 0.6291271092189916 1103 9 0.7689827344743563 1103 10-11 0.9218890297405409 1103 12-13 0.8043631456500577 1103 14-15 0.9958189814587541 1103 16-17 1.1704818722686525 1103 18-19 1.2458888004197348 1103 20-21 0.9269188056421598 1103 22-23 1.1262440633540152 1103 24-25 1.0676782487926175 1103 26-27 0.968966959498708 1103 28-29 1.1711287068756846 1103 30-31 1.1351888570417117 1103 32-33 0.9384827534944762 1103 34-35 0.8766160215683385 1103 36-37 0.9012570099451338 1103 38-39 0.9537988608954038 1103 40-41 0.7815754266570849 1103 42-43 0.8507265627570177 1103 44-45 0.8949257549885701 1103 46-47 0.9226787943706043 1103 48-49 0.9307621307007139 1103 50-51 0.9531148595894123 1103 52-53 0.919160450734438 1103 54-55 0.831649076194303 1103 56-57 0.7080654292683377 1103 58-59 0.7437676278168581 1103 60-61 0.7058105753945441 1103 62-63 0.6820612277755558 1103 64-65 0.7316211587660106 1103 66-67 0.7784226784760904 1103 68-69 0.7646241944533472 1103 70-71 0.7623809434305215 1103 72-73 0.7027775479588527 1103 74-75 0.6392287585396872 1103 76-77 0.6677979712665092 1103 78-79 0.632556247203155 1103 80-81 0.6848124740444632 1103 82-83 0.6046834590171528 1103 84-85 0.576768676928932 1103 86-87 0.630917180161326 1103 88-89 0.6638677144677771 1103 90-91 0.6681767598202839 1103 92-93 0.6598890474189822 1103 94-95 0.696764328024436 1103 96-97 0.7201719099959227 1103 98-99 0.8017320022784276 1103 100 0.823784286967733 1104 1 0.6656187276540919 1104 2 0.7898931543447567 1104 3 0.7280230879661573 1104 4 0.9053071094740872 1104 5 0.7644745288050387 1104 6 0.49601867949875356 1104 7 0.5863352899569563 1104 8 0.5956703283709075 1104 9 0.6642603367937241 1104 10-11 0.6982769302205938 1104 12-13 0.6310449559119249 1104 14-15 0.8494757397949542 1104 16-17 0.9695329698940469 1104 18-19 0.9066992641288465 1104 20-21 0.7826794630980345 1104 22-23 1.0478038687350875 1104 24-25 0.9397697129677667 1104 26-27 0.801031296749187 1104 28-29 1.0320592780975133 1104 30-31 1.0376154412953227 1104 32-33 0.870747619528359 1104 34-35 0.74843875853729 1104 36-37 0.818906264271483 1104 38-39 0.959333851828859 1104 40-41 0.8130847783833559 1104 42-43 0.8051545808462066 1104 44-45 0.8365294713893334 1104 46-47 0.8787721023828254 1104 48-49 0.8194718821979308 1104 50-51 0.8000221886078549 1104 52-53 0.8519497880599687 1104 54-55 0.7715495670069501 1104 56-57 0.75280517559532 1104 58-59 0.7597005486716384 1104 60-61 0.7821607215053419 1104 62-63 0.7242924636279113 1104 64-65 0.7057981507500415 1104 66-67 0.8079682649616728 1104 68-69 0.7958426833373977 1104 70-71 0.6924247698454948 1104 72-73 0.6243688266859095 1104 74-75 0.6104285903283468 1104 76-77 0.68629561405033 1104 78-79 0.599141310602306 1104 80-81 0.7002734311687107 1104 82-83 0.690541401764861 1104 84-85 0.6171909410393717 1104 86-87 0.61953507705406 1104 88-89 0.5523434773791465 1104 90-91 0.6052798949632248 1104 92-93 0.6404635696157719 1104 94-95 0.6329724953275004 1104 96-97 0.5968030997328455 1104 98-99 0.6334653089385291 1104 100 0.7570170875395519 1105 1 0.5404754794161768 1105 2 0.6906936206926524 1105 3 0.7052260776698738 1105 4 0.7797661650993675 1105 5 0.7191094057737217 1105 6 0.37446648671070193 1105 7 0.5087319542356141 1105 8 0.5056173972933777 1105 9 0.5563963349032477 1105 10-11 0.6398519318286162 1105 12-13 0.5715831284179274 1105 14-15 0.6722684814873361 1105 16-17 0.8354722306566771 1105 18-19 0.8122663543834392 1105 20-21 0.636238517637679 1105 22-23 0.9653400251136901 1105 24-25 0.7768414211163517 1105 26-27 0.6142055839244378 1105 28-29 0.8673090180803982 1105 30-31 0.8840468125533931 1105 32-33 0.7687880754650607 1105 34-35 0.6749021532743171 1105 36-37 0.675223130716212 1105 38-39 0.7755092944066178 1105 40-41 0.6422830719223711 1105 42-43 0.6777857634685631 1105 44-45 0.7617276939535671 1105 46-47 0.6033286452167843 1105 48-49 0.6391699736105778 1105 50-51 0.7033592640709685 1105 52-53 0.6663646941804515 1105 54-55 0.6313411042681878 1105 56-57 0.5897327454028485 1105 58-59 0.62253162764123 1105 60-61 0.6826311979223902 1105 62-63 0.6500042319276176 1105 64-65 0.569473932734347 1105 66-67 0.6547940443252784 1105 68-69 0.6152334119219418 1105 70-71 0.5691475863582909 1105 72-73 0.5839016737096969 1105 74-75 0.4959239520835794 1105 76-77 0.5298441698477703 1105 78-79 0.6166345416354488 1105 80-81 0.6093673727123345 1105 82-83 0.5799026516899062 1105 84-85 0.5334142974313387 1105 86-87 0.6102215047497239 1105 88-89 0.6205902531921126 1105 90-91 0.6385959009554583 1105 92-93 0.6546450624823557 1105 94-95 0.6281686913749454 1105 96-97 0.6379226156225819 1105 98-99 0.6620930006444681 1105 100 0.6841215900061393 1106 1 0.5215445685127484 1106 2 0.5630482339584297 1106 3 0.6174094686915268 1106 4 0.748975850404328 1106 5 0.604540866864312 1106 6 0.3027431484258898 1106 7 0.3533144669686834 1106 8 0.30312436730348225 1106 9 0.5466096889508663 1106 10-11 0.4828663114669496 1106 12-13 0.4166010988487514 1106 14-15 0.6042884267904753 1106 16-17 0.762561842546333 1106 18-19 0.7441635960744222 1106 20-21 0.4880643861016125 1106 22-23 0.8002396713563229 1106 24-25 0.6831493402115925 1106 26-27 0.551605159526531 1106 28-29 0.6981002168655053 1106 30-31 0.7106720691345316 1106 32-33 0.5334992042348645 1106 34-35 0.5702810144726982 1106 36-37 0.48131889130785765 1106 38-39 0.5940652644444029 1106 40-41 0.46023684949766874 1106 42-43 0.4751410513586265 1106 44-45 0.5655992168287582 1106 46-47 0.5673901815838889 1106 48-49 0.5597328049962798 1106 50-51 0.649479232551144 1106 52-53 0.5947104940505454 1106 54-55 0.5726104519004309 1106 56-57 0.5397637434498179 1106 58-59 0.5726156710924712 1106 60-61 0.5240148069733763 1106 62-63 0.49527162554745985 1106 64-65 0.5504158688765841 1106 66-67 0.6062259495411126 1106 68-69 0.5496502525318832 1106 70-71 0.5871928181398403 1106 72-73 0.5597558159519238 1106 74-75 0.5642535401686786 1106 76-77 0.5237363786971159 1106 78-79 0.5368169253722641 1106 80-81 0.5656845978667917 1106 82-83 0.4933651130232448 1106 84-85 0.5332310076466662 1106 86-87 0.5374927595896111 1106 88-89 0.5561571042984568 1106 90-91 0.5069929399124504 1106 92-93 0.5779786783778071 1106 94-95 0.5662557192368993 1106 96-97 0.5704953772466439 1106 98-99 0.46681876832790437 1106 100 0.4380352342837739 1107 1 0.4415438326445873 1107 2 0.6233591789320023 1107 3 0.5993437373424051 1107 4 0.7543437698398989 1107 5 0.6347980902900616 1107 6 0.38424319702042453 1107 7 0.3517817938904457 1107 8 0.3581012756149633 1107 9 0.509600595251058 1107 10-11 0.5133883186921508 1107 12-13 0.42650590486081796 1107 14-15 0.6729926907602675 1107 16-17 0.7782487084022875 1107 18-19 0.8352647278131684 1107 20-21 0.5833791000672832 1107 22-23 0.8319546190161162 1107 24-25 0.5305169612324718 1107 26-27 0.5071577522052024 1107 28-29 0.6056344079143798 1107 30-31 0.6586018214132068 1107 32-33 0.5837490738300275 1107 34-35 0.45430962847489553 1107 36-37 0.42889196022719034 1107 38-39 0.4960706115416116 1107 40-41 0.34711003987816724 1107 42-43 0.5232600844524882 1107 44-45 0.4334481068236897 1107 46-47 0.43020610836067874 1107 48-49 0.43933022018099166 1107 50-51 0.5359749359971673 1107 52-53 0.5018316361791335 1107 54-55 0.47655960855988155 1107 56-57 0.39819895972686226 1107 58-59 0.40593560804290263 1107 60-61 0.5163932632984682 1107 62-63 0.45630468220443987 1107 64-65 0.4949994453925086 1107 66-67 0.492529448476688 1107 68-69 0.4769113170812247 1107 70-71 0.33623744427132607 1107 72-73 0.426239748865747 1107 74-75 0.4733486243920879 1107 76-77 0.35672663962003526 1107 78-79 0.35890139247711517 1107 80-81 0.47250721335680623 1107 82-83 0.45791709907712885 1107 84-85 0.48267860929017914 1107 86-87 0.46398623751954204 1107 88-89 0.5106688465359284 1107 90-91 0.5487655578811825 1107 92-93 0.6057430830950743 1107 94-95 0.4819760331369132 1107 96-97 0.4788772626726079 1107 98-99 0.4979680546523966 1107 100 0.5234723641352304 1108 1 0.4096895754185468 1108 2 0.4922257221741404 1108 3 0.45085135077089333 1108 4 0.5844152849066475 1108 5 0.4858278998899337 1108 6 0.2777736783392797 1108 7 0.24567719344361194 1108 8 0.31908947544491184 1108 9 0.46361357322972196 1108 10-11 0.45049816958893985 1108 12-13 0.30602778817679166 1108 14-15 0.5427897672184514 1108 16-17 0.7146566037687165 1108 18-19 0.7912382786484926 1108 20-21 0.4780213591042042 1108 22-23 0.674862426046893 1108 24-25 0.5464578922901779 1108 26-27 0.4915372587033957 1108 28-29 0.6019404504406296 1108 30-31 0.696029763450305 1108 32-33 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2215 94-95 -2.238338211769708 2215 96-97 -1.7930845960311466 2215 98-99 -3.2961105562566324 2215 100 -3.2761219415227885 2216 1 -0.9203015002303196 2216 2 -0.9032305485053094 2216 3 -1.1547998093947527 2216 4 -1.1935081984827747 2216 5 -1.2798594162437382 2216 6 -1.0547676100125045 2216 7 -1.264063365439469 2216 8 -1.1257796575878842 2216 9 -1.3671206222029895 2216 10-11 -1.3873842018192732 2216 12-13 -1.5677495051698003 2216 14-15 -1.987777987301925 2216 16-17 -1.7432060494091246 2216 18-19 -3.628763343960806 2216 20-21 -1.931569320063609 2216 22-23 -1.44437223147672 2216 24-25 -1.5845263789818702 2216 26-27 -1.602220580968698 2216 28-29 -1.5072385445292795 2216 30-31 -1.6975637164822572 2216 32-33 -1.6704782771527817 2216 34-35 -1.839633834248282 2216 36-37 -1.876128299782458 2216 38-39 -1.8958840404613966 2216 40-41 -1.951479261837136 2216 42-43 -1.9277349607703513 2216 44-45 -1.8265054825094822 2216 46-47 -2.048059682660927 2216 48-49 -2.019679683579035 2216 50-51 -1.8638902413676277 2216 52-53 -1.912836881326072 2216 54-55 -1.775844400208662 2216 56-57 -1.8568310368608891 2216 58-59 -1.8769812421843923 2216 60-61 -1.81980354468935 2216 62-63 -1.8639828362098356 2216 64-65 -1.8418430818619953 2216 66-67 -1.701977601797644 2216 68-69 -1.929354122940115 2216 70-71 -2.06359206963241 2216 72-73 -1.9610279174158265 2216 74-75 -1.8917790977217734 2216 76-77 -1.7931075577235163 2216 78-79 -1.9231254531065503 2216 80-81 -1.8415083882032164 2216 82-83 -1.9967558505440266 2216 84-85 -1.9112451936501564 2216 86-87 -1.8754061872093999 2216 88-89 -1.7242745432073505 2216 90-91 -1.7713025764813253 2216 92-93 -1.7325034807620021 2216 94-95 -1.7140747837669679 2216 96-97 -1.6387942959935842 2216 98-99 -1.6180623360552602 2216 100 -1.672280614434733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 19221.0 1 3308.0 2 512.0 3 477.0 4 421.0 5 284.0 6 211.0 7 279.0 8 543.0 9 951.0 10 1646.0 11 3265.0 12 5512.0 13 7963.0 14 10484.0 15 13193.0 16 16296.0 17 18987.0 18 21920.0 19 24897.0 20 28591.0 21 32250.0 22 37009.0 23 42511.0 24 48150.0 25 53717.0 26 60516.0 27 69385.0 28 82313.0 29 97645.0 30 115970.0 31 138762.0 32 170481.0 33 217028.0 34 296216.0 35 402198.0 36 590972.0 37 919082.0 38 826134.0 39 283289.0 40 342.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64843658444602 20.639312156509867 12.696302897561498 24.01594836148262 2 15.365069558037128 19.721168965376844 41.11990841781829 23.79385305876775 3 18.626096693498194 26.365468528840303 28.92271036947516 26.085724408186344 4 12.091609172121645 16.80160826714817 39.900539105627644 31.206243455102545 5 13.18195868016926 38.305570304346844 35.17841968825423 13.334051327229668 6 30.015236839099412 38.24763419517378 18.33190016529923 13.40522880042758 7 27.036669085851905 31.617400286206788 23.64236610542575 17.703564522515556 8 25.738803089094382 35.34694542696209 20.24412183328918 18.670129650654353 9 25.996728718398792 15.332014386395402 21.4419638555074 37.229293039698405 10-11 25.356789827852772 25.92518046076479 27.91041327905756 20.80761643232488 12-13 25.668795096167425 23.768678558992498 27.801522421817136 22.761003923022937 14-15 23.028447517061128 25.080428903845913 25.178550926806814 26.71257265228614 16-17 22.230234674164283 27.632548642494914 26.48163400834857 23.65558267499224 18-19 21.779820604404726 28.153448304857807 27.921452654991853 22.145278435745613 20-21 23.363109307229685 27.282591388336 26.878486062004647 22.47581324242967 22-23 23.695391079588834 26.954745969747307 26.656109350660408 22.69375360000345 24-25 23.14039946948407 27.080643445149672 27.296549587425005 22.48240749794125 26-27 23.019494593864604 27.26395594299819 26.50354671539471 23.213002747742493 28-29 22.297618982177152 27.745554959495394 26.97635710664853 22.980468951678922 30-31 22.982699839921324 27.381635178203002 26.86956880726133 22.766096174614354 32-33 23.365849709644284 27.62218325637117 26.024027632003165 22.98793940198138 34-35 23.396239433074296 27.385665904304606 26.873268042943106 22.344826619677995 36-37 23.11284287515578 27.01179194879955 26.693665307569503 23.181699868475164 38-39 23.148854727275513 27.1280216679687 27.175114697618113 22.548008907137678 40-41 22.875286782469693 26.40439716408405 27.676218588970407 23.044097464475847 42-43 22.76568228583504 26.6951661098853 27.051110858341776 23.488040745937884 44-45 22.930051413169366 27.431110978842867 26.908299181708855 22.73053842627891 46-47 22.99291925226079 27.121488267096726 26.546070362376557 23.339522118265922 48-49 23.45891926356984 26.784507565284365 26.77431020980574 22.98226296134006 50-51 23.145927430335963 26.792846403288856 26.735717074952515 23.325509091422667 52-53 22.71120922545011 27.276436647042363 26.569804270086667 23.442549857420865 54-55 22.648813258761212 27.16951099024551 26.606726929842605 23.574948821150677 56-57 23.551393466798444 26.656445064222968 26.460540000724414 23.331621468254173 58-59 22.69046868511417 26.961576160522505 27.15399937127449 23.193955783088835 60-61 22.73126673399883 27.569049153664043 26.540999368397156 23.15868474393997 62-63 23.044428866954643 26.958756457828294 26.262275223056275 23.73453945216079 64-65 22.360203651404525 27.09423529225326 26.620714621217424 23.92484643512479 66-67 23.00347868851858 26.702408325308312 26.577292396873414 23.71682058929969 68-69 22.493134756312422 26.867374317626908 26.75750022751161 23.881990698549057 70-71 22.53790284065121 26.573699638517457 26.905006527816262 23.983390993015075 72-73 23.0274918679715 26.6986697337171 27.215393557188772 23.058444841122625 74-75 23.168914846178335 27.032856924618937 26.810930077788136 22.98729815141459 76-77 22.884282437103977 26.93154105786983 26.532735405126328 23.651441099899866 78-79 22.786983654140506 26.90751745203474 26.608687850899415 23.696811042925336 80-81 22.51171703695335 27.15540009978185 27.128082985383294 23.204799877881502 82-83 23.16932791073278 26.356924003027952 27.927431357410192 22.54631672882908 84-85 23.349898958884072 26.88234898385326 26.72866341966606 23.03908863759661 86-87 22.293665359537346 27.412557676932614 27.1560847805354 23.137692182994645 88-89 23.171974207349642 26.335440648299173 26.986676226247162 23.50590891810402 90-91 22.90164631184512 26.41418664561793 27.20203967455416 23.48212736798279 92-93 23.170204528030293 26.650055366783114 27.050725904986418 23.129014200200174 94-95 22.19423190877661 26.59951304589198 27.63576786894418 23.570487176387225 96-97 22.49721629219879 26.56266568075886 27.21878588055698 23.72133214648537 98-99 22.008452375673524 27.201922356578105 27.06829888594345 23.721326381804918 100 22.668384205036094 26.54266768169397 26.43876542786935 24.350182685400583 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16205.0 1 9545.5 2 2679.5 3 1941.5 4 1078.0 5 632.5 6 490.5 7 447.0 8 450.5 9 466.0 10 437.0 11 413.5 12 422.0 13 426.0 14 406.5 15 522.5 16 647.5 17 752.5 18 957.5 19 1199.0 20 1567.0 21 2074.5 22 2960.0 23 4177.0 24 5625.0 25 7461.0 26 10125.0 27 13472.0 28 18684.0 29 28005.0 30 40851.0 31 53410.5 32 68842.5 33 83637.5 34 94587.5 35 106719.0 36 119395.0 37 139922.0 38 149195.5 39 141935.0 40 134629.5 41 127044.0 42 126483.0 43 134784.0 44 146005.5 45 168014.0 46 190846.5 47 205565.0 48 214168.0 49 210148.5 50 199611.5 51 199902.5 52 200574.0 53 192912.5 54 183092.5 55 161596.0 56 142837.5 57 120849.0 58 98613.0 59 90944.5 60 76076.0 61 55990.5 62 43359.5 63 35724.0 64 26392.0 65 15396.5 66 10033.5 67 7106.0 68 4995.5 69 3586.0 70 2765.5 71 2167.0 72 1610.5 73 1298.5 74 977.5 75 708.0 76 505.0 77 298.5 78 185.5 79 112.5 80 70.0 81 54.5 82 47.0 83 36.0 84 27.5 85 21.5 86 15.5 87 11.5 88 8.0 89 7.5 90 7.5 91 7.0 92 9.5 93 9.0 94 5.0 95 5.0 96 4.0 97 1.0 98 0.5 99 0.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9979131151629737 2 0.449931598816281 3 0.48508116461513157 4 0.4698975815854878 5 0.436763915228426 6 0.49027103339080075 7 0.5067199150062482 8 0.5098081013851589 9 0.522890002018044 10-11 0.5363900945564066 12-13 0.5345564838939285 14-15 0.532465524366541 16-17 0.5363793716870355 18-19 0.5342776892902769 20-21 0.5341597377271935 22-23 0.4970800554415238 24-25 0.5319508266367227 26-27 0.5114808690070688 28-29 0.5176465188955187 30-31 0.5205845851032322 32-33 0.5232009652298093 34-35 0.5347173269344968 36-37 0.5529569277349375 38-39 0.542620081661084 40-41 0.5465982661978056 42-43 0.5450112815308655 44-45 0.5552194531722644 46-47 0.5528604219105966 48-49 0.5248522871129767 50-51 0.5406041822192951 52-53 0.5319615495060939 54-55 0.5419981552375535 56-57 0.5300314330192748 58-59 0.5356502165697927 60-61 0.5303852877085249 62-63 0.5248093956354919 64-65 0.5341597377271935 66-67 0.5325513073215109 68-69 0.5534072882485286 70-71 0.537987802092718 72-73 0.5415156261158486 74-75 0.5391780405929232 76-77 0.5271684268971597 78-79 0.5577500503438717 80-81 0.5321331154160334 82-83 0.532111669677291 84-85 0.5407543023904922 86-87 0.8458306588709977 88-89 1.1119615537952416 90-91 1.1025468744873128 92-93 1.1025575973566841 94-95 1.1068896365826557 96-97 1.0996516997570842 98-99 1.095694960959105 100 1.0916738849448984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4662931.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.01353185767901 #Duplication Level Percentage of deduplicated Percentage of total 1 74.00092211395489 26.65034566048538 2 13.326667393285948 9.598807214495912 3 5.110757065803898 5.521692372185604 4 2.490018723692905 3.5869747452772662 5 1.3441294234261008 2.4203423905699797 6 0.8404107072946695 1.8159694668414674 7 0.5575438860835272 1.4055387152466285 8 0.3811085399269535 1.0980051635114296 9 0.2789272016679996 0.9040638296919387 >10 1.3226782152164074 8.628561893222875 >50 0.1335558590529842 3.4043383978008963 >100 0.16312862656973817 12.887964174885669 >500 0.031174111371237323 7.891224140412688 >1k 0.018145236548932596 11.606663350324729 >5k 5.949257884895934E-4 1.4979767867484495 >10k+ 2.3797031539583735E-4 1.08153169829903 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 14395 0.3087114091973482 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 13666 0.2930774656541133 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 11279 0.24188648727592155 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11139 0.23888408385198068 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 9766 0.20943908455861773 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 9535 0.20448511890911533 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8794 0.18859382650097117 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7712 0.16538953718165678 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6088 0.13056165746394274 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5964 0.12790238585988084 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 5822 0.1248570909584551 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 5632 0.12078240059739251 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5371 0.11518506278561703 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 5232 0.11220410510041859 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4976 0.1067139959823553 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 4957 0.10630652694624905 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4798 0.1028966544862019 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 4798 0.1028966544862019 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.578295496973899E-5 2 0.0 0.0 0.0 0.0 8.578295496973899E-5 3 0.0 0.0 0.0 0.0 8.578295496973899E-5 4 0.0 0.0 0.0 0.0 8.578295496973899E-5 5 0.0 0.0 0.0 0.0 8.578295496973899E-5 6 6.433721622730424E-5 0.0 0.0 0.0 1.0722869371217373E-4 7 6.433721622730424E-5 0.0 0.0 0.0 1.0722869371217373E-4 8 6.433721622730424E-5 0.0 0.0 0.0 1.0722869371217373E-4 9 6.433721622730424E-5 0.0 0.0 0.0 1.0722869371217373E-4 10-11 1.0722869371217373E-4 0.0 0.0 0.0 1.2867443245460848E-4 12-13 1.2867443245460848E-4 0.0 0.0 0.0 2.7879460365165174E-4 14-15 1.608430405682606E-4 0.0 0.0 0.0 3.3240895050773856E-4 16-17 2.0373451805313008E-4 0.0 0.0 0.0 3.645775586213907E-4 18-19 2.1445738742434747E-4 0.0 0.0 1.0722869371217373E-5 3.9674616673504283E-4 20-21 2.1445738742434747E-4 0.0 0.0 4.2891477484869494E-5 5.146977298184339E-4 22-23 2.3590312616678222E-4 0.0 0.0 8.578295496973899E-5 5.146977298184339E-4 24-25 3.645775586213907E-4 0.0 0.0 1.608430405682606E-4 5.146977298184339E-4 26-27 4.7180625233356443E-4 0.0 0.0 3.4313181987895595E-4 5.683120766745208E-4 28-29 4.932519910759992E-4 0.0 0.0 0.0015869846669401713 6.219264235306077E-4 30-31 5.575892073033035E-4 0.0 0.0 0.006197818496563642 6.433721622730424E-4 32-33 6.219264235306077E-4 0.0 0.0 0.016513218831674756 6.862636397579119E-4 34-35 7.398779866139987E-4 0.0 0.0 0.03284414888403882 7.291551172427814E-4 36-37 8.578295496973899E-4 0.0 0.0 0.061602884537643815 7.291551172427814E-4 38-39 8.578295496973899E-4 0.0 0.0 0.10829025777992426 8.256609415837378E-4 40-41 9.221667659246942E-4 0.0 0.0 0.1586877438246459 8.363838109549551E-4 42-43 9.221667659246942E-4 0.0 0.0 0.2129132942348922 8.685524190686073E-4 44-45 9.328896352959115E-4 0.0 0.0 0.2801242394536827 9.328896352959115E-4 46-47 9.436125046671289E-4 0.0 0.0 0.3672689988335663 9.865039821519983E-4 48-49 9.436125046671289E-4 0.0 0.0 0.4700905932341697 0.0010508411983793026 50-51 9.436125046671289E-4 0.0 0.0 0.581243857136209 0.0010722869371217374 52-53 9.75781112780781E-4 0.0 0.0 0.6927938672049834 0.0011366241533490417 54-55 9.865039821519983E-4 0.0 0.0 0.8202887840287578 0.001179515630833911 56-57 9.865039821519983E-4 0.0 0.0 0.9783117099523884 0.0011902385002051283 58-59 9.865039821519983E-4 0.0 0.0 1.1696398681430198 0.0012009613695763458 60-61 0.001007949720894433 0.0 0.0 1.3692031900107464 0.0012224071083187806 62-63 0.001007949720894433 0.0 0.0 1.5753825222805142 0.0013296358020309543 64-65 0.001007949720894433 0.0 0.0 1.793110384863083 0.0013403586714021715 66-67 0.0010293954596368679 0.0 0.0 2.0469528714879117 0.001383250148887041 68-69 0.0010508411983793026 0.0 0.0 2.335355166096174 0.0014154187570006934 70-71 0.0010722869371217374 0.0 0.0 2.654553541538573 0.0014154187570006934 72-73 0.0010722869371217374 0.0 0.0 2.9891499573980402 0.0014797559732279975 74-75 0.0010722869371217374 0.0 0.0 3.3292579281143126 0.0015548160588265193 76-77 0.0010722869371217374 0.0 0.0 3.7128364112615007 0.0015655389281977366 78-79 0.0010937326758641722 0.0 0.0 4.135371078834321 0.0015869846669401713 80-81 0.0010937326758641722 0.0 0.0 4.590953629809234 0.0015977075363113888 82-83 0.001115178414606607 0.0 0.0 5.065161804881951 0.0016513218831674756 84-85 0.0011366241533490417 0.0 0.0 5.534083605354658 0.0016513218831674756 86-87 0.0011366241533490417 0.0 0.0 6.034359075868805 0.0016942133606523452 88 0.0011366241533490417 0.0 0.0 6.440562813389261 0.0017371048381372145 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4250 0.0 33.334892 94 GTACTAG 4375 0.0 31.057503 1 TAGGCAT 5240 0.0 29.857653 5 GCATCGT 4365 0.0 29.253363 8 GTATCAA 11115 0.0 28.311865 1 CTAGGCA 6015 0.0 27.807188 4 TACCCCG 345 0.0 27.236647 5 CAACGCA 10330 0.0 26.15232 5 GGTATCA 7620 0.0 26.128204 1 TAGTACT 6065 0.0 25.951157 4 ATCAACG 10765 0.0 25.18473 3 CTGTGCG 6010 0.0 25.1539 9 GTACATG 18100 0.0 24.814781 1 TCTAACG 1920 0.0 24.473331 2 TACATGG 18450 0.0 24.271177 2 AGGGCTA 5175 0.0 24.240614 5 CTATTGA 4940 0.0 24.234673 9 ACATGGG 17885 0.0 24.222418 3 CTAACGC 1960 0.0 24.212568 3 GCTATTG 5070 0.0 23.704082 8 >>END_MODULE