FastQCFastQC Report
Mon 16 Jan 2017
ERR1146443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4662931
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC152450.3269402871284177No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT122110.2618739157838707No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA120780.2590216325311269No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC103710.22241375649779074No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA103600.222177853371624No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC102250.21928267864139528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99050.2124200422438162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93140.19974561064703722No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC85410.18316805459913518No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG62690.13444333617632342No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC61760.13244888247327702No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT60820.13043298303148815No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT56760.12172601310205962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.11623590398399633No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG53390.11449879914585911No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG52750.11312627186634329No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC52330.11222555083916104No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC50910.10918025593773531No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT50340.10795784882941652No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC50250.10776483718073461No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT49990.10720724797343131No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA49980.10718580223468886No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT48510.10403327863955096No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA48370.10373303829715687No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA46740.10023738288214001No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA46710.10017304566591272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108350.040.578821
GTATCAA176850.036.00231
GGGCACC43950.034.89863294
GTACTAG45800.030.9042111
CAACGCA201050.030.4118615
ATCAACG204850.029.835973
TAGTACT58700.028.2722324
TAGGCAT53300.028.215815
TATCAAC224700.028.2054862
AACGCAG225800.027.8895386
CTGTGCG59450.027.6726749
TCAACGC235150.026.2108554
ACTGTGC68700.025.8580368
ACGCAGA237850.025.6664897
GTACTGT67300.025.4881466
CGCAGAG238800.025.485668
TATTCTC27700.025.4496155
CTAGTAC69200.025.2737483
CTAGGCA61550.025.2063164
CTAACGC18200.025.0572343
Produced by FastQC (version 0.11.5)