##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4662931
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847071294857248	31.0	31.0	34.0	30.0	34.0
2	31.845748307234228	31.0	31.0	34.0	30.0	34.0
3	32.02326626750428	33.0	31.0	34.0	30.0	34.0
4	35.74594584393378	37.0	35.0	37.0	35.0	37.0
5	35.65226399447043	37.0	35.0	37.0	33.0	37.0
6	35.684448257973365	37.0	35.0	37.0	33.0	37.0
7	35.60636732561559	37.0	35.0	37.0	33.0	37.0
8	35.591293115853524	37.0	35.0	37.0	33.0	37.0
9	37.196207063754535	39.0	37.0	39.0	33.0	39.0
10-11	37.14247562316491	39.0	37.0	39.0	33.0	39.0
12-13	37.072707702515864	39.0	37.0	39.0	33.0	39.0
14-15	38.27232045681139	40.0	38.0	41.0	33.0	41.0
16-17	38.278081961753244	40.0	38.0	41.0	33.0	41.0
18-19	38.339897909705286	40.0	38.0	41.0	33.0	41.0
20-21	38.35659588357623	40.0	38.0	41.0	33.0	41.0
22-23	38.32556261287161	40.0	38.0	41.0	33.0	41.0
24-25	38.25927597899261	40.0	37.5	41.0	33.0	41.0
26-27	38.11859214729963	40.0	37.0	41.0	33.0	41.0
28-29	38.054563535252825	40.0	37.0	41.0	33.0	41.0
30-31	37.92402503918673	40.0	37.0	41.0	33.0	41.0
32-33	37.79962956775471	40.0	36.5	41.0	33.0	41.0
34-35	37.69955066888187	40.0	36.0	41.0	32.5	41.0
36-37	37.615159220670435	40.0	36.0	41.0	32.0	41.0
38-39	37.50585833245228	40.0	36.0	41.0	32.0	41.0
40-41	37.386469368729664	40.0	36.0	41.0	31.0	41.0
42-43	37.26387962849976	40.0	36.0	41.0	31.0	41.0
44-45	37.07693615882371	39.0	35.0	41.0	31.0	41.0
46-47	36.83867764717084	39.0	35.0	41.0	30.5	41.0
48-49	36.58850248909967	39.0	35.0	41.0	30.0	41.0
50-51	36.39867167238803	39.0	35.0	41.0	30.0	41.0
52-53	36.218379598582956	39.0	35.0	41.0	29.0	41.0
54-55	36.026517227040245	38.0	35.0	40.0	29.0	41.0
56-57	35.78448994848948	38.0	34.5	40.0	28.5	41.0
58-59	35.51929902029431	37.5	34.0	40.0	28.0	41.0
60-61	35.266308572869725	37.0	34.0	40.0	28.0	41.0
62-63	35.00084796450987	36.5	34.0	40.0	28.0	41.0
64-65	34.73315657040604	36.0	34.0	39.0	27.0	41.0
66-67	34.505137219487054	35.5	34.0	39.0	27.0	41.0
68-69	34.311798523289326	35.0	33.0	39.0	27.0	41.0
70-71	34.086784685426395	35.0	33.0	38.5	27.0	40.5
72-73	33.87160768623855	35.0	33.0	37.5	26.0	40.0
74-75	33.67929259515099	35.0	33.0	37.0	26.5	39.5
76-77	33.50334692921684	35.0	33.0	36.5	27.0	39.0
78-79	33.368946698975385	35.0	33.0	36.0	27.0	39.0
80-81	33.204075934213904	35.0	33.0	36.0	27.0	38.5
82-83	33.00663691999732	35.0	33.0	35.5	27.0	37.0
84-85	32.85048052394514	35.0	33.0	35.0	27.0	37.0
86-87	32.70417072437915	35.0	33.0	35.0	26.5	36.5
88-89	32.58293485363605	35.0	33.0	35.0	26.0	36.0
90-91	32.488270467652214	35.0	33.0	35.0	26.0	36.0
92-93	32.40810747574862	35.0	33.0	35.0	26.0	36.0
94-95	32.354744901865374	35.0	33.0	35.0	26.0	35.5
96-97	32.295271471956156	35.0	33.0	35.0	26.0	35.0
98-99	32.255708051438035	35.0	33.0	35.0	26.0	35.0
100	31.770705592684088	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48449730847066874
1101	2	0.6051711188802642
1101	3	0.4919874970905411
1101	4	0.33043410557097275
1101	5	0.34784705125360915
1101	6	0.4185837126697365
1101	7	0.4361117312453118
1101	8	0.40256911888469205
1101	9	0.49854071992696447
1101	10-11	0.5674845159311488
1101	12-13	0.517038764300807
1101	14-15	0.7020719315613135
1101	16-17	0.6587346704133736
1101	18-19	0.6478100934653028
1101	20-21	0.6527953410650724
1101	22-23	0.6480040129863056
1101	24-25	0.6958020592576233
1101	26-27	0.680555382215779
1101	28-29	0.6962975140371341
1101	30-31	0.7821971917033252
1101	32-33	0.7868951899578249
1101	34-35	0.776172460754978
1101	36-37	0.7893798733432504
1101	38-39	0.8030552787682055
1101	40-41	0.7722713972063744
1101	42-43	0.797434884945261
1101	44-45	0.7820295721397699
1101	46-47	0.80329654804342
1101	48-49	0.8109831430955481
1101	50-51	0.7703628664901174
1101	52-53	0.8534482438585087
1101	54-55	0.7133697635666749
1101	56-57	0.7308776175536451
1101	58-59	0.8135163016471907
1101	60-61	0.7828309586919246
1101	62-63	0.793578996490254
1101	64-65	0.7850739673505842
1101	66-67	0.7764625065585591
1101	68-69	0.7195756105399482
1101	70-71	0.6575485357790427
1101	72-73	0.6602130741247905
1101	74-75	0.6285734034419548
1101	76-77	0.647951376413971
1101	78-79	0.6274697282344732
1101	80-81	0.590883270361374
1101	82-83	0.6066492685543494
1101	84-85	0.6521720103268649
1101	86-87	0.6959692356919831
1101	88-89	0.6139514708387068
1101	90-91	0.6028713423418566
1101	92-93	0.638281119691321
1101	94-95	0.6047348409943822
1101	96-97	0.6393038957158694
1101	98-99	0.6256420130365683
1101	100	0.6662877097956503
1102	1	0.42987589052415487
1102	2	0.5262290808500083
1102	3	0.43916373388732666
1102	4	0.33892881802911035
1102	5	0.30090457370278756
1102	6	0.33478403394206424
1102	7	0.2991597437833846
1102	8	0.3572576380132162
1102	9	0.4937734859503067
1102	10-11	0.45499331416305466
1102	12-13	0.4950943511389738
1102	14-15	0.6247842544040267
1102	16-17	0.6211435989373157
1102	18-19	0.600010159784425
1102	20-21	0.5652992122871083
1102	22-23	0.5536820594816447
1102	24-25	0.5945574418203918
1102	26-27	0.6226007247837444
1102	28-29	0.6297840930186709
1102	30-31	0.7001736022749654
1102	32-33	0.6679337879967235
1102	34-35	0.6816271299179917
1102	36-37	0.6350146794614986
1102	38-39	0.6352542658375384
1102	40-41	0.618981679641351
1102	42-43	0.665677979701826
1102	44-45	0.6531800133026664
1102	46-47	0.6769544266450183
1102	48-49	0.7001905670827355
1102	50-51	0.7107542001143798
1102	52-53	0.6628465151819043
1102	54-55	0.733314240857851
1102	56-57	0.7245774969592134
1102	58-59	0.6551291622378415
1102	60-61	0.6717825691984984
1102	62-63	0.6229073942110048
1102	64-65	0.6494608557170594
1102	66-67	0.6600823494260482
1102	68-69	0.6182395696973799
1102	70-71	0.5913775614980281
1102	72-73	0.5403901688469404
1102	74-75	0.5272108896971588
1102	76-77	0.504829188915437
1102	78-79	0.5076207801901873
1102	80-81	0.4603099619289921
1102	82-83	0.48163214132956966
1102	84-85	0.5210319980015186
1102	86-87	0.5398781610876711
1102	88-89	0.5323812054214656
1102	90-91	0.5687876566882082
1102	92-93	0.5365096054726024
1102	94-95	0.5306149965627611
1102	96-97	0.5006325577746082
1102	98-99	0.5246320737717411
1102	100	0.6189460662390083
1103	1	0.25451797481469995
1103	2	-0.18330708713362398
1103	3	0.16920477021250235
1103	4	0.19040302052406588
1103	5	0.23538519279730252
1103	6	0.24704486551651428
1103	7	0.2778147679362064
1103	8	0.2834690617516813
1103	9	0.37879345920336505
1103	10-11	0.3754519424866416
1103	12-13	0.3972943847224002
1103	14-15	0.4806096947614549
1103	16-17	0.4665225825496506
1103	18-19	0.4593707297056895
1103	20-21	0.43640355840437195
1103	22-23	0.3707942669816475
1103	24-25	0.392930635672343
1103	26-27	0.4542725478017644
1103	28-29	0.4831386874852157
1103	30-31	0.5305278842880625
1103	32-33	0.5346565301051456
1103	34-35	0.5637401847329926
1103	36-37	0.5217053124859987
1103	38-39	0.5218702250010168
1103	40-41	0.5186477196620558
1103	42-43	0.5607604223882703
1103	44-45	0.5716360024864073
1103	46-47	0.5892770822635498
1103	48-49	0.6488438966055696
1103	50-51	0.6326448390903607
1103	52-53	0.634626230286969
1103	54-55	0.5742388305960446
1103	56-57	0.5638961397634858
1103	58-59	0.522915946481298
1103	60-61	0.5759756963434768
1103	62-63	0.5379021064630223
1103	64-65	0.5644083521379457
1103	66-67	0.5844836107563509
1103	68-69	0.56141907043272
1103	70-71	0.5121520054729345
1103	72-73	0.4977059947394551
1103	74-75	0.5390409432354915
1103	76-77	0.47507623002771027
1103	78-79	0.48977222021023437
1103	80-81	0.488297375291765
1103	82-83	0.5214169426433841
1103	84-85	0.42666611134772126
1103	86-87	0.42315604697782305
1103	88-89	0.4758109490956386
1103	90-91	0.43740801725213885
1103	92-93	0.3928492607226346
1103	94-95	0.4137460853712298
1103	96-97	0.4425403614008019
1103	98-99	0.5303864440138071
1103	100	0.46386585729518615
1104	1	0.37573862443313644
1104	2	0.4300197665957697
1104	3	0.3892522915534613
1104	4	0.271703848905652
1104	5	0.2573505277089936
1104	6	0.2828032846511874
1104	7	0.2762046855175626
1104	8	0.2748756589002994
1104	9	0.37067411570636466
1104	10-11	0.3411653335853728
1104	12-13	0.4158567481681388
1104	14-15	0.4496022888073057
1104	16-17	0.47656142976237703
1104	18-19	0.47488531530611766
1104	20-21	0.4820302897064437
1104	22-23	0.4477940662306281
1104	24-25	0.4394233372285683
1104	26-27	0.4677559085679519
1104	28-29	0.49391580835693816
1104	30-31	0.49458771121020106
1104	32-33	0.47955561012432923
1104	34-35	0.49263407898964573
1104	36-37	0.5188539969391215
1104	38-39	0.495676525495071
1104	40-41	0.42877678396854435
1104	42-43	0.44834338131322227
1104	44-45	0.473128932709308
1104	46-47	0.44522616067057186
1104	48-49	0.4576997066515176
1104	50-51	0.4842630427824304
1104	52-53	0.47111125191790393
1104	54-55	0.44933993226257485
1104	56-57	0.47325568124800554
1104	58-59	0.4395469615504126
1104	60-61	0.4522430272988558
1104	62-63	0.4618767917948716
1104	64-65	0.4832370722981665
1104	66-67	0.3897853499837822
1104	68-69	0.39972629060348197
1104	70-71	0.3227073274666097
1104	72-73	0.3443116124071395
1104	74-75	0.34978246384432055
1104	76-77	0.37279699834618185
1104	78-79	0.31847415337352203
1104	80-81	0.3209685272302565
1104	82-83	0.340910421978208
1104	84-85	0.2983860847717068
1104	86-87	0.31477186341317775
1104	88-89	0.3211316861766349
1104	90-91	0.2805879040042356
1104	92-93	0.2682704470001198
1104	94-95	0.25668508259943223
1104	96-97	0.32849502060032165
1104	98-99	0.32332833027878394
1104	100	0.42499293175322705
1105	1	0.11386851054001568
1105	2	-0.40786365724791906
1105	3	0.012078567273597685
1105	4	0.10140186482976077
1105	5	0.14491880834763293
1105	6	0.2028897859442793
1105	7	0.2632756990938603
1105	8	0.2522239056539348
1105	9	0.35108065956564616
1105	10-11	0.3245868075580418
1105	12-13	0.32438567364069115
1105	14-15	0.3938545650101801
1105	16-17	0.4017161783273977
1105	18-19	0.42025472445156
1105	20-21	0.4269606980382932
1105	22-23	0.37666143012335596
1105	24-25	0.40690514575783254
1105	26-27	0.4472509479015585
1105	28-29	0.4299248831675797
1105	30-31	0.46960267175246173
1105	32-33	0.43262395420835986
1105	34-35	0.45235504420465134
1105	36-37	0.4588709873056587
1105	38-39	0.4856026397359088
1105	40-41	0.48037665632288906
1105	42-43	0.5065560682188277
1105	44-45	0.43517756140765584
1105	46-47	0.3796611440158131
1105	48-49	0.4241306778982121
1105	50-51	0.3912376828003943
1105	52-53	0.31968350119083055
1105	54-55	0.32494727930423295
1105	56-57	0.30583803018563316
1105	58-59	0.31852702125561194
1105	60-61	0.3210163375435897
1105	62-63	0.3613035031149465
1105	64-65	0.3193993674202602
1105	66-67	0.3406454405957007
1105	68-69	0.29060021001340886
1105	70-71	0.32256458994360315
1105	72-73	0.3521501816676107
1105	74-75	0.2867822477681301
1105	76-77	0.2553521402842591
1105	78-79	0.24822026794198848
1105	80-81	0.2647877366404572
1105	82-83	0.24557149424450841
1105	84-85	0.2400256796566822
1105	86-87	0.25509061333559657
1105	88-89	0.25319596006707457
1105	90-91	0.2208133049679759
1105	92-93	0.22289606300499543
1105	94-95	0.24553857878253638
1105	96-97	0.21923482140944373
1105	98-99	0.2592489492660377
1105	100	0.23451033475796024
1106	1	0.2229292260901623
1106	2	0.2631319882328853
1106	3	0.20719689926259832
1106	4	0.14948700374371526
1106	5	0.20121731737306447
1106	6	0.17843452555346317
1106	7	0.19583407258539864
1106	8	0.21004061690936737
1106	9	0.21630320843893003
1106	10-11	0.2069578146273514
1106	12-13	0.23291832161352488
1106	14-15	0.23012677553409588
1106	16-17	0.25237787929705036
1106	18-19	0.3252483540914355
1106	20-21	0.3022030499513235
1106	22-23	0.26296762033134513
1106	24-25	0.24263742571806546
1106	26-27	0.3200767869535994
1106	28-29	0.2976635928317748
1106	30-31	0.3277094223348769
1106	32-33	0.3037389634054932
1106	34-35	0.37199668840646893
1106	36-37	0.37809355547868506
1106	38-39	0.29091621174928406
1106	40-41	0.31002408524739167
1106	42-43	0.28673937996129695
1106	44-45	0.3179830727442621
1106	46-47	0.3740848470137834
1106	48-49	0.3021718971820704
1106	50-51	0.2612258527507123
1106	52-53	0.32086968426138185
1106	54-55	0.26187548466091215
1106	56-57	0.2944221738048469
1106	58-59	0.3423854919566054
1106	60-61	0.24828713011417136
1106	62-63	0.31756690278566424
1106	64-65	0.32023809731450115
1106	66-67	0.2994721525277768
1106	68-69	0.3375928042855776
1106	70-71	0.34391900553146826
1106	72-73	0.2779136584506219
1106	74-75	0.27114494797336874
1106	76-77	0.2810037536706318
1106	78-79	0.23313425320103676
1106	80-81	0.2860308031062857
1106	82-83	0.24241849968409213
1106	84-85	0.3053904167938768
1106	86-87	0.2926109047917862
1106	88-89	0.1942353576053435
1106	90-91	0.16152249775387162
1106	92-93	0.17288352106055527
1106	94-95	0.23864383190127825
1106	96-97	0.2883855731546987
1106	98-99	0.29479448397844976
1106	100	0.30101413238708474
1107	1	0.05240836595356413
1107	2	-0.4169416030614208
1107	3	-0.02962247329523393
1107	4	0.0671961394256897
1107	5	0.08658532711294953
1107	6	0.1426079891585985
1107	7	0.1482491876085561
1107	8	0.157438264112713
1107	9	0.18506610863791195
1107	10-11	0.26421768674936175
1107	12-13	0.259132505483592
1107	14-15	0.28456512313169213
1107	16-17	0.31781749326329845
1107	18-19	0.3120521612837521
1107	20-21	0.24981415303020782
1107	22-23	0.30199097588010204
1107	24-25	0.30160720245204686
1107	26-27	0.3251068488659712
1107	28-29	0.25395648492929723
1107	30-31	0.30400414962491595
1107	32-33	0.3280760957328539
1107	34-35	0.36757735602286346
1107	36-37	0.3762850344574531
1107	38-39	0.41650257170893923
1107	40-41	0.44283026531601166
1107	42-43	0.41158473150132124
1107	44-45	0.3887266281558581
1107	46-47	0.46445895444012564
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2216	76-77	-0.8483871005489974
2216	78-79	-0.7957233380224054
2216	80-81	-0.7592316699607409
2216	82-83	-0.7055046343567568
2216	84-85	-0.7263368244593877
2216	86-87	-0.6608813994822071
2216	88-89	-0.6490418937704234
2216	90-91	-0.6984367837387602
2216	92-93	-0.6699309224708756
2216	94-95	-0.6424727014778
2216	96-97	-0.73797580609261
2216	98-99	-0.7521009993924928
2216	100	-0.7754924164444965
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	11.0
13	71.0
14	291.0
15	873.0
16	2063.0
17	4095.0
18	7328.0
19	11577.0
20	16652.0
21	23488.0
22	32185.0
23	38056.0
24	40418.0
25	41986.0
26	46049.0
27	55558.0
28	69721.0
29	87225.0
30	108459.0
31	133661.0
32	166967.0
33	210755.0
34	285985.0
35	393982.0
36	585399.0
37	999708.0
38	944658.0
39	354870.0
40	840.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.313938259895615	21.130626439466376	13.924318332640546	24.631116967997464
2	14.836575374782852	20.34913247689107	40.16381066763892	24.650481480687155
3	17.986286060855093	26.246604274169837	29.92191251396411	25.84519715101096
4	12.20183431132666	17.36745819984872	38.64770487984133	31.783002608983296
5	12.702322406334574	38.44661343533801	35.14741057559279	13.70365358273464
6	29.800741207622416	38.79688547825391	17.66957306466684	13.732800249456833
7	26.136115059529374	32.34831139709383	23.604883476148363	17.910690067228433
8	25.896630252517138	35.23461959870305	19.74181046213208	19.126939686647734
9	25.886271960704544	14.77997422651118	21.29840651727422	38.03534729551006
10-11	25.306078944766714	26.081213725873276	27.584506397371094	21.028200931988913
12-13	25.913089857001957	23.80906987472043	27.98004087986719	22.29779938841042
14-15	23.08049163589912	24.82897912082008	25.865125810305617	26.22540343297518
16-17	21.718501825300454	27.417284572297213	27.082613986378124	23.781599616024213
18-19	21.28713678156507	27.150058621926853	29.622001698073593	21.940802898434484
20-21	22.67026554872854	26.322122176627378	29.027717446725283	21.9798948279188
22-23	22.95021989014768	25.984310395909493	28.81460053852793	22.250869175414895
24-25	22.45506339843581	26.123421740898777	29.44557076284967	21.97594409781574
26-27	22.359724224424085	26.315119575236302	28.660129849909566	22.66502635043005
28-29	21.66605081653578	26.886147360962447	29.043138317937796	22.404663504563974
30-31	22.428178757095054	26.401291376604114	28.935427095103915	22.235102771196917
32-33	22.832838401426056	26.619115745011023	28.21062546282585	22.33742039073707
34-35	22.765338118329137	26.50010969547122	28.870625611608546	21.863926574591098
36-37	22.580990368504274	26.079691078422563	28.702065288978112	22.63725326409505
38-39	22.62155498333559	26.118379191113917	29.19556390604965	22.064501919500845
40-41	22.29834538982194	25.608359241684003	29.530887464933386	22.562407903560672
42-43	22.424465396611893	25.7670233262762	28.76596432774825	23.042546949363658
44-45	22.566096302947653	26.51840655587655	28.473721785718038	22.441775355457757
46-47	22.748942671465652	26.395736930269827	27.759771697243647	23.09554870102088
48-49	23.380189654222143	26.208875707793446	27.69919065509992	22.711743982884496
50-51	22.92905470829399	26.353735450942768	27.433571288101838	23.283638552661408
52-53	22.374232001288462	26.85280996008734	27.354275669101686	23.418682369522518
54-55	22.45474610940712	26.76358603225365	27.209980711632255	23.571687146706974
56-57	23.371030348014425	26.251100570039256	27.02849896025552	23.349370121690797
58-59	22.442739742478963	26.5311542981651	27.79604839885737	23.230057560498572
60-61	22.522089936437986	27.126162078685994	27.158759633033032	23.19298835184299
62-63	22.825707693294195	26.430843604591193	26.904397684632265	23.83905101748235
64-65	22.08707355952726	26.590657249699813	27.244934570123387	24.077334620649545
66-67	22.681581606075664	26.229339443367273	27.20396248625596	23.885116464301102
68-69	22.31845896444128	26.147875410061776	27.48094009594317	24.052725529553776
70-71	22.325733362170954	25.923801985056265	27.55891436867325	24.19155028409953
72-73	22.774527437785373	26.038107791000982	27.79265873760517	23.394706033608475
74-75	22.884968456467522	26.437430529061075	27.326004450858164	23.351596563613235
76-77	22.65733035944068	26.37676932315347	26.929688170198162	24.036212147207685
78-79	22.39876119971301	26.44372379111405	27.122411092585434	24.035103916587506
80-81	22.124147746099272	26.83241960863287	27.535776380758016	23.507656264509844
82-83	22.841773580515255	25.970111482785928	28.3215173625874	22.866597574111417
84-85	23.055132005250677	26.47882801929767	27.087732444937533	23.37830753051412
86-87	21.96824463348976	27.026345344677765	27.415284831767504	23.590125190064963
88-89	22.632375438949435	26.056122043009484	27.30717484605537	24.004327671985713
90-91	22.345030371693337	26.072129621636638	27.514260942951402	24.06857906371863
92-93	22.631890954801783	26.416850419741998	27.16626484421364	23.78499378124258
94-95	21.616224438204807	26.3276102506143	27.76950969285366	24.28665561832723
96-97	21.92542213229811	26.22894003191715	27.426380054290465	24.41925778149427
98-99	21.398301722425934	26.893823552245603	27.274364003755647	24.43351072157281
100	22.05390605665603	26.24072297726723	26.59606001944212	25.109310946634622
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	153.5
2	147.0
3	197.0
4	148.5
5	119.5
6	250.5
7	675.5
8	1164.5
9	1342.5
10	1121.5
11	810.5
12	780.5
13	884.5
14	1084.0
15	1481.5
16	1855.5
17	2414.5
18	3259.5
19	4142.5
20	5205.0
21	6410.5
22	7784.0
23	9194.5
24	10923.5
25	12767.0
26	15042.5
27	17832.0
28	22467.0
29	31358.5
30	43354.0
31	54567.5
32	69502.0
33	83479.0
34	92626.0
35	104457.0
36	116850.0
37	137139.0
38	147028.0
39	139702.5
40	132281.0
41	124895.0
42	124567.5
43	131901.0
44	142481.5
45	165472.5
46	188436.0
47	204185.0
48	214963.0
49	209858.5
50	198202.0
51	198601.0
52	198652.5
53	191112.5
54	183952.5
55	162896.0
56	143173.0
57	121418.0
58	98563.5
59	92747.0
60	77825.0
61	56414.5
62	43140.0
63	34663.5
64	25464.0
65	14467.0
66	9210.5
67	6551.5
68	4529.5
69	3122.5
70	2418.0
71	1907.0
72	1395.5
73	1157.0
74	919.0
75	649.5
76	438.0
77	248.5
78	120.5
79	49.5
80	24.0
81	12.5
82	6.5
83	4.5
84	4.5
85	2.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013146237849112499
2	0.004953965649502426
3	0.0018014420543645189
4	0.028844518608574735
5	0.0029595119464559953
6	0.0
7	4.932519910759992E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008910704447481639
16-17	0.0029380662077135604
18-19	0.0
20-21	3.216860811365212E-5
22-23	7.398779866139988E-4
24-25	1.0722869371217373E-5
26-27	1.9301164868191272E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.718062523335645E-4
36-37	0.0
38-39	0.0
40-41	4.2891477484869494E-4
42-43	7.506008559852162E-4
44-45	0.0
46-47	0.0
48-49	5.146977298184339E-4
50-51	0.0
52-53	0.0
54-55	3.645775586213907E-4
56-57	1.3939730182582584E-4
58-59	2.3590312616678224E-4
60-61	9.650582434095636E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0014261416263719108
70-71	8.685524190686072E-4
72-73	0.0
74-75	2.1445738742434747E-4
76-77	0.0028093917752589517
78-79	6.969865091291293E-4
80-81	0.0031739693338803427
82-83	0.0022196339598419966
84-85	0.006315770059647033
86-87	0.008160103591496421
88-89	0.008106489244640334
90-91	0.006079866933480251
92-93	0.009092993226792332
94-95	0.001897947878705475
96-97	0.005018302865729731
98-99	0.0019086707480766927
100	0.08522536576243568
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4662931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.420825592487226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.05570357722497	19.72834159927058
2	16.476963201586855	9.695317212954336
3	6.4349038955175395	5.6796055564331445
4	3.272459133706107	3.8511379772523684
5	1.821678837632998	2.679764768376264
6	1.1056590451957924	1.951764116007681
7	0.7507993738821719	1.5462396203755189
8	0.5282825715828559	1.243400752167188
9	0.36337197583905545	0.9621633173722499
>10	1.7243952381693441	9.171723005428172
>50	0.18094866717496258	3.7403624680619765
>100	0.2151563178426638	14.015267954425237
>500	0.04169028261539787	8.660003242464853
>1k	0.02653017984033622	13.668195302928742
>5k	0.001020391532320624	1.8951677746009497
>10k+	4.3731065670883884E-4	1.511545331880753
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	15245	0.3269402871284177	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12211	0.2618739157838707	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12078	0.2590216325311269	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10371	0.22241375649779074	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10360	0.222177853371624	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10225	0.21928267864139528	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9905	0.2124200422438162	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9314	0.19974561064703722	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8541	0.18316805459913518	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6269	0.13444333617632342	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6176	0.13244888247327702	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6082	0.13043298303148815	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5676	0.12172601310205962	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5420	0.11623590398399633	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5339	0.11449879914585911	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5275	0.11312627186634329	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5233	0.11222555083916104	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5091	0.10918025593773531	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5034	0.10795784882941652	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5025	0.10776483718073461	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4999	0.10720724797343131	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4998	0.10718580223468886	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4851	0.10403327863955096	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4837	0.10373303829715687	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4674	0.10023738288214001	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4671	0.10017304566591272	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1445738742434747E-5
2	0.0	0.0	0.0	0.0	2.1445738742434747E-5
3	0.0	0.0	0.0	0.0	2.1445738742434747E-5
4	0.0	0.0	0.0	0.0	2.1445738742434747E-5
5	2.1445738742434747E-5	0.0	0.0	0.0	2.1445738742434747E-5
6	2.1445738742434747E-5	0.0	0.0	0.0	6.433721622730424E-5
7	2.1445738742434747E-5	0.0	0.0	0.0	6.433721622730424E-5
8	2.1445738742434747E-5	0.0	0.0	0.0	6.433721622730424E-5
9	2.1445738742434747E-5	0.0	0.0	0.0	6.433721622730424E-5
10-11	1.5012017119704323E-4	0.0	0.0	0.0	8.578295496973899E-5
12-13	2.1445738742434747E-4	0.0	0.0	0.0	2.466259955379996E-4
14-15	2.5734886490921696E-4	0.0	0.0	0.0	3.002403423940865E-4
16-17	3.5385468925017334E-4	0.0	0.0	0.0	3.4313181987895595E-4
18-19	4.074690361062602E-4	0.0	0.0	1.0722869371217373E-5	3.4313181987895595E-4
20-21	4.074690361062602E-4	0.0	0.0	4.2891477484869494E-5	4.1819190547747755E-4
22-23	4.074690361062602E-4	0.0	0.0	8.578295496973899E-5	4.7180625233356443E-4
24-25	4.932519910759992E-4	0.0	0.0	1.608430405682606E-4	4.7180625233356443E-4
26-27	5.468663379320861E-4	0.0	0.0	3.4313181987895595E-4	4.932519910759992E-4
28-29	5.575892073033035E-4	0.0	0.0	0.001576261797568954	4.932519910759992E-4
30-31	6.004806847881729E-4	0.0	0.0	0.006219264235306077	5.039748604472165E-4
32-33	6.326492929018251E-4	0.0	0.0	0.01662044752538693	5.575892073033035E-4
34-35	7.291551172427814E-4	0.0	0.0	0.03304788340209194	5.575892073033035E-4
36-37	8.149380722125204E-4	0.0	0.0	0.06222481096117442	5.575892073033035E-4
38-39	8.149380722125204E-4	0.0	0.0	0.10936254471704598	6.112035541593903E-4
40-41	8.363838109549551E-4	0.0	0.0	0.16021039127535877	6.219264235306077E-4
42-43	8.363838109549551E-4	0.0	0.0	0.21543316853712824	6.326492929018251E-4
44-45	8.578295496973899E-4	0.0	0.0	0.2840809782516619	6.755407703866946E-4
46-47	8.792752884398247E-4	0.0	0.0	0.37271621647414466	7.291551172427814E-4
48-49	8.792752884398247E-4	0.0	0.0	0.47704973545608975	7.506008559852161E-4
50-51	8.792752884398247E-4	0.0	0.0	0.5897470925475843	8.363838109549551E-4
52-53	9.221667659246942E-4	0.0	0.0	0.703141436148208	8.363838109549551E-4
54-55	9.436125046671289E-4	0.0	0.0	0.8317193627784756	8.792752884398247E-4
56-57	9.436125046671289E-4	0.0	0.0	0.990975418679796	8.89998157811042E-4
58-59	9.436125046671289E-4	0.0	0.0	1.1845661023077545	9.007210271822594E-4
60-61	9.543353740383463E-4	0.0	0.0	1.3862096608334973	9.007210271822594E-4
62-63	9.650582434095636E-4	0.0	0.0	1.5942440495044856	9.650582434095636E-4
64-65	9.650582434095636E-4	0.0	0.0	1.8147598581235709	0.0010293954596368679
66-67	9.650582434095636E-4	0.0	0.0	2.070553906973961	0.0010401183290080851
68-69	0.0010293954596368679	0.0	0.0	2.3618084848349676	0.0010508411983793026
70-71	0.0010293954596368679	0.0	0.0	2.6845561300392395	0.0010722869371217374
72-73	0.0010293954596368679	0.0	0.0	3.0229162730480033	0.001115178414606607
74-75	0.0010293954596368679	0.0	0.0	3.3673991744677325	0.0011580698920914765
76-77	0.0010293954596368679	0.0	0.0	3.757904631228727	0.0012009613695763458
78-79	0.0010508411983793026	0.0	0.0	4.1874091638928395	0.0012224071083187806
80-81	0.0010508411983793026	0.0	0.0	4.652020370878317	0.0012224071083187806
82-83	0.0010508411983793026	0.0	0.0	5.134678167015553	0.001286744324546085
84-85	0.0010508411983793026	0.0	0.0	5.612392720372658	0.001286744324546085
86-87	0.0010508411983793026	0.0	0.0	6.122125761672218	0.0013403586714021718
88	0.0010508411983793026	0.0	0.0	6.53709008346896	0.0013939730182582586
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10835	0.0	40.57882	1
GTATCAA	17685	0.0	36.0023	1
GGGCACC	4395	0.0	34.898632	94
GTACTAG	4580	0.0	30.904211	1
CAACGCA	20105	0.0	30.411861	5
ATCAACG	20485	0.0	29.83597	3
TAGTACT	5870	0.0	28.272232	4
TAGGCAT	5330	0.0	28.21581	5
TATCAAC	22470	0.0	28.205486	2
AACGCAG	22580	0.0	27.889538	6
CTGTGCG	5945	0.0	27.672674	9
TCAACGC	23515	0.0	26.210855	4
ACTGTGC	6870	0.0	25.858036	8
ACGCAGA	23785	0.0	25.666489	7
GTACTGT	6730	0.0	25.488146	6
CGCAGAG	23880	0.0	25.48566	8
TATTCTC	2770	0.0	25.449615	5
CTAGTAC	6920	0.0	25.273748	3
CTAGGCA	6155	0.0	25.206316	4
CTAACGC	1820	0.0	25.057234	3
>>END_MODULE