##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146442_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3846127
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.431200529779698	31.0	30.0	34.0	26.0	34.0
2	30.48655673616602	31.0	30.0	34.0	26.0	34.0
3	30.71912445948873	31.0	31.0	34.0	27.0	34.0
4	34.18845503541615	37.0	35.0	37.0	30.0	37.0
5	34.39233363848879	37.0	35.0	37.0	30.0	37.0
6	34.60282512771939	37.0	35.0	37.0	31.0	37.0
7	34.44810298775885	37.0	35.0	37.0	30.0	37.0
8	34.412547739583225	37.0	35.0	37.0	30.0	37.0
9	35.798030070249894	39.0	35.0	39.0	30.0	39.0
10-11	35.714018803851246	39.0	35.0	39.0	29.5	39.0
12-13	35.70338680963993	39.0	35.0	39.0	30.0	39.0
14-15	36.70835856434278	40.0	36.0	41.0	29.5	41.0
16-17	36.54364065461177	40.0	36.0	41.0	28.0	41.0
18-19	36.494694142965116	40.0	36.0	41.0	27.5	41.0
20-21	36.83925413799388	40.0	36.0	41.0	30.0	41.0
22-23	36.555781569355354	40.0	36.0	41.0	28.0	41.0
24-25	36.66880890828618	40.0	36.0	41.0	29.5	41.0
26-27	36.745627874482565	40.0	36.0	41.0	29.5	41.0
28-29	36.59229336420768	40.0	35.5	41.0	29.5	41.0
30-31	36.54795512472677	39.0	35.0	41.0	29.5	41.0
32-33	36.652701405855815	40.0	35.0	41.0	30.0	41.0
34-35	36.69861941636353	40.0	35.0	41.0	30.0	41.0
36-37	36.62855607732142	40.0	35.0	41.0	30.0	41.0
38-39	36.45479634447848	39.0	35.0	41.0	29.0	41.0
40-41	36.41368407751486	39.0	35.0	41.0	29.0	41.0
42-43	36.25333991831263	39.0	35.0	41.0	28.0	41.0
44-45	36.05968354659116	39.0	35.0	41.0	28.0	41.0
46-47	35.92463457394932	38.5	34.5	40.5	28.0	41.0
48-49	35.79798066990507	38.0	34.0	40.0	28.0	41.0
50-51	35.568025574818506	38.0	34.0	40.0	27.0	41.0
52-53	35.37992115184964	38.0	34.0	40.0	27.0	41.0
54-55	35.1845260179916	37.5	33.5	40.0	27.0	41.0
56-57	35.03509231494436	37.0	33.0	40.0	27.0	41.0
58-59	34.773285047529626	37.0	33.0	40.0	26.0	41.0
60-61	34.52587278059201	36.0	33.0	40.0	26.0	41.0
62-63	34.2600269049878	35.5	33.0	39.0	26.0	41.0
64-65	33.96925101537209	35.0	32.5	39.0	26.0	41.0
66-67	33.685077351839915	35.0	32.0	38.5	26.0	40.5
68-69	33.39867417274573	35.0	32.0	38.0	25.0	40.0
70-71	33.34164394467473	35.0	32.0	37.5	25.5	40.0
72-73	33.13877947868076	35.0	32.0	37.0	25.0	40.0
74-75	32.8809998993793	35.0	32.0	36.5	25.0	39.0
76-77	32.65507561242777	35.0	32.0	36.0	25.0	39.0
78-79	32.444640673591906	35.0	31.5	36.0	25.0	39.0
80-81	32.23697735410193	35.0	31.0	35.5	24.0	37.5
82-83	32.008705120761746	35.0	31.0	35.0	24.0	37.0
84-85	31.837508745811046	35.0	31.0	35.0	24.0	37.0
86-87	31.680460629615197	34.5	31.0	35.0	24.0	36.0
88-89	31.68901117929803	35.0	31.0	35.0	24.0	36.0
90-91	31.62039566036171	35.0	31.0	35.0	24.0	36.0
92-93	31.483710626300173	35.0	31.0	35.0	24.0	36.0
94-95	31.47456493246323	35.0	31.0	35.0	24.0	35.0
96-97	31.389866871270762	35.0	31.0	35.0	23.5	35.0
98-99	31.321409693439662	35.0	31.0	35.0	23.0	35.0
100	30.83783478808682	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8281262876670858
1101	2	0.968023957206956
1101	3	0.9636685897764217
1101	4	1.009692322109018
1101	5	0.9687954289453131
1101	6	0.7717555184420419
1101	7	0.806619936767305
1101	8	0.889174812019732
1101	9	1.115359545097057
1101	10-11	1.0827784082566012
1101	12-13	0.9893263644656614
1101	14-15	1.2439430070594
1101	16-17	1.3190265558123073
1101	18-19	1.4882722532202806
1101	20-21	1.0949484711372293
1101	22-23	1.4315400919019012
1101	24-25	1.2269628458812107
1101	26-27	1.153433925445647
1101	28-29	1.362580944547659
1101	30-31	1.3702256807543165
1101	32-33	1.2320603646051183
1101	34-35	1.102073507645862
1101	36-37	1.0620968381096816
1101	38-39	1.170890894370018
1101	40-41	1.0345042588498643
1101	42-43	1.1406196633342631
1101	44-45	1.1360823286175048
1101	46-47	1.1512076963659652
1101	48-49	1.0800732079450057
1101	50-51	1.1478390727442047
1101	52-53	1.136332988304126
1101	54-55	1.138912747654686
1101	56-57	0.9877793821696201
1101	58-59	0.8941355648015374
1101	60-61	0.9153165493163442
1101	62-63	0.9414610340969034
1101	64-65	0.8551249372303431
1101	66-67	0.98552661209024
1101	68-69	0.9839521136759046
1101	70-71	0.9062868140249094
1101	72-73	0.8772034270435043
1101	74-75	0.8983849662354331
1101	76-77	0.9056344342105689
1101	78-79	0.8552419577518222
1101	80-81	0.8828697053622037
1101	82-83	0.948422306206627
1101	84-85	0.9185035848389695
1101	86-87	0.9053338219989229
1101	88-89	0.8797306220209045
1101	90-91	0.8957628436698748
1101	92-93	0.9012817885158206
1101	94-95	0.8767223875943735
1101	96-97	0.856836623664158
1101	98-99	0.9367798912673138
1101	100	1.0224200578283522
1102	1	0.7119746628684034
1102	2	0.8700201640082135
1102	3	0.8587105209138528
1102	4	0.8747294009366584
1102	5	0.823581082341164
1102	6	0.6500951069161189
1102	7	0.6744755713702517
1102	8	0.6987660356192933
1102	9	0.8802555455611198
1102	10-11	0.8479610417591488
1102	12-13	0.8329474804688459
1102	14-15	1.0537982482381523
1102	16-17	1.1763958682801174
1102	18-19	1.1923602540511027
1102	20-21	0.9371529267014012
1102	22-23	1.1709119224483402
1102	24-25	1.1190161594216974
1102	26-27	0.992053355595715
1102	28-29	1.1940286865511993
1102	30-31	1.245447888240676
1102	32-33	1.0618209816495892
1102	34-35	1.0300738232974354
1102	36-37	0.9237389958753539
1102	38-39	0.9452034194926142
1102	40-41	0.8799012073797101
1102	42-43	1.0190514094395127
1102	44-45	1.02989412203425
1102	46-47	0.9762412584677591
1102	48-49	0.8837201473818936
1102	50-51	1.0402100698956218
1102	52-53	0.9855134432377
1102	54-55	0.9823008527943813
1102	56-57	0.88950127967653
1102	58-59	0.8242552282109941
1102	60-61	0.8059654823052327
1102	62-63	0.8503195051199413
1102	64-65	0.800048677375301
1102	66-67	0.8136864288602936
1102	68-69	0.8843269952480881
1102	70-71	0.7934278948444557
1102	72-73	0.7050999864178777
1102	74-75	0.7674341912751288
1102	76-77	0.7622070158884924
1102	78-79	0.8571157369994395
1102	80-81	0.8805831026141107
1102	82-83	0.894598463296564
1102	84-85	0.9292772855771751
1102	86-87	0.8793433964517021
1102	88-89	0.8480718881615026
1102	90-91	0.8277023863925983
1102	92-93	0.921614255941666
1102	94-95	0.8703788024262771
1102	96-97	0.9100305229693149
1102	98-99	0.9024428116327172
1102	100	0.8659923320412801
1103	1	0.6007736123856091
1103	2	0.7608815984363488
1103	3	0.7832109805284091
1103	4	0.8171939324075055
1103	5	0.7295349894049465
1103	6	0.5640074299135094
1103	7	0.6307966033551224
1103	8	0.6485397420395032
1103	9	0.7950534341677837
1103	10-11	0.8179745921710975
1103	12-13	0.796248731459201
1103	14-15	0.9597969833063402
1103	16-17	1.1913264273777102
1103	18-19	1.2414187809496084
1103	20-21	0.9099358956867718
1103	22-23	1.1390222378765458
1103	24-25	1.0236727463047188
1103	26-27	0.8882431735747502
1103	28-29	1.0927918360047073
1103	30-31	1.1378098706925783
1103	32-33	0.8839320689582166
1103	34-35	0.95550147452537
1103	36-37	0.8310790007038591
1103	38-39	0.9459910832338494
1103	40-41	0.9055607344357668
1103	42-43	0.9351354253939803
1103	44-45	1.004496507269451
1103	46-47	0.9462585776057395
1103	48-49	0.8848142680559334
1103	50-51	0.9247622602338552
1103	52-53	0.9491733182633411
1103	54-55	0.9033342544748422
1103	56-57	0.8651597866262861
1103	58-59	0.8426522785169652
1103	60-61	0.8080820045135155
1103	62-63	0.7357181003202271
1103	64-65	0.8108891769553566
1103	66-67	0.8253090099332425
1103	68-69	0.8230229707020627
1103	70-71	0.7372115370999879
1103	72-73	0.7452230339060861
1103	74-75	0.7570413200046033
1103	76-77	0.7692724421059012
1103	78-79	0.7132002460052789
1103	80-81	0.7888371226829989
1103	82-83	0.6706433921781532
1103	84-85	0.647011946862218
1103	86-87	0.6574851538228188
1103	88-89	0.6799293827124337
1103	90-91	0.6856637492999518
1103	92-93	0.771456904545655
1103	94-95	0.6905760003076331
1103	96-97	0.6937974581506268
1103	98-99	0.7264982215397637
1103	100	0.6994369136333489
1104	1	0.5770946985375858
1104	2	0.7939224305422918
1104	3	0.7155842996512192
1104	4	0.8506247306365751
1104	5	0.7011908068911552
1104	6	0.5060431687207583
1104	7	0.5485030295586597
1104	8	0.5304000556569903
1104	9	0.6766892288795745
1104	10-11	0.7395393468579741
1104	12-13	0.5828630084441073
1104	14-15	0.8990094121290255
1104	16-17	1.034421388676023
1104	18-19	0.9755308649017209
1104	20-21	0.7907383960492496
1104	22-23	0.9732914007990559
1104	24-25	0.8558058270022215
1104	26-27	0.7856263239350056
1104	28-29	0.9548505671518228
1104	30-31	0.9611471537610328
1104	32-33	0.8032863424737826
1104	34-35	0.688405959411817
1104	36-37	0.7092472902196363
1104	38-39	0.8527489657302922
1104	40-41	0.7123502157816901
1104	42-43	0.6785699980511382
1104	44-45	0.6982385548923418
1104	46-47	0.690766639452896
1104	48-49	0.624915169356818
1104	50-51	0.6899590113781713
1104	52-53	0.7160494574563501
1104	54-55	0.6963634320534027
1104	56-57	0.6557564775076017
1104	58-59	0.6782772427412596
1104	60-61	0.6371461904210989
1104	62-63	0.6204020871274523
1104	64-65	0.652673548731002
1104	66-67	0.6999497201184681
1104	68-69	0.5762979626316422
1104	70-71	0.5477898004039758
1104	72-73	0.5563476304214703
1104	74-75	0.5503063484900679
1104	76-77	0.6003032096514005
1104	78-79	0.5234425154738815
1104	80-81	0.5774427518981824
1104	82-83	0.5137464456875698
1104	84-85	0.5411326867198589
1104	86-87	0.5169089186564761
1104	88-89	0.45146619965789725
1104	90-91	0.48305385867119455
1104	92-93	0.5323749535469666
1104	94-95	0.512025720654183
1104	96-97	0.46806704529676324
1104	98-99	0.5395260276556115
1104	100	0.7427134194020617
1105	1	0.48647419264804626
1105	2	0.6515549308392004
1105	3	0.6215917888024585
1105	4	0.7063840181719598
1105	5	0.620678916263401
1105	6	0.3049878355031055
1105	7	0.35764288710865344
1105	8	0.41591874838165666
1105	9	0.4680344901470619
1105	10-11	0.583808510845671
1105	12-13	0.5371822197338503
1105	14-15	0.6100769806115451
1105	16-17	0.8045397418323077
1105	18-19	0.8550347798534901
1105	20-21	0.6267878817204462
1105	22-23	0.8734296996199618
1105	24-25	0.7652417431838145
1105	26-27	0.5606833657239676
1105	28-29	0.8198116470221635
1105	30-31	0.8403709932515255
1105	32-33	0.7027941940212088
1105	34-35	0.6373127216617931
1105	36-37	0.5277683807898228
1105	38-39	0.6185962605972151
1105	40-41	0.5020440677738378
1105	42-43	0.5430804413140251
1105	44-45	0.5878107571993141
1105	46-47	0.5061917307364112
1105	48-49	0.5596366829630455
1105	50-51	0.721051457369299
1105	52-53	0.6584898803739989
1105	54-55	0.6154460424750923
1105	56-57	0.5849754517506511
1105	58-59	0.5501417942523901
1105	60-61	0.606518070326814
1105	62-63	0.5762350748370579
1105	64-65	0.5732677156877912
1105	66-67	0.6009097462835129
1105	68-69	0.5770691875973597
1105	70-71	0.4817658027096954
1105	72-73	0.4719711054068796
1105	74-75	0.49973441367919236
1105	76-77	0.4979914956524709
1105	78-79	0.544636865275173
1105	80-81	0.6038436564272232
1105	82-83	0.5572510073657675
1105	84-85	0.4326361887730634
1105	86-87	0.5380222426638284
1105	88-89	0.5085150182537284
1105	90-91	0.5756406274920245
1105	92-93	0.5664727535301672
1105	94-95	0.5227338897931233
1105	96-97	0.49364159772174787
1105	98-99	0.47930055395995197
1105	100	0.6165828968374889
1106	1	0.5557921966120887
1106	2	0.5223244853117208
1106	3	0.5065480252738759
1106	4	0.7109367838174379
1106	5	0.6001324555652445
1106	6	0.384744031874952
1106	7	0.40792218304078887
1106	8	0.48167761266315523
1106	9	0.581995801988775
1106	10-11	0.5466648383889208
1106	12-13	0.44417150223725343
1106	14-15	0.6785796982052545
1106	16-17	0.7543507580167628
1106	18-19	0.7607420486934231
1106	20-21	0.5854354609400829
1106	22-23	0.905731489297267
1106	24-25	0.691842600458024
1106	26-27	0.5511403120029286
1106	28-29	0.7348579759543554
1106	30-31	0.7175457852652727
1106	32-33	0.6367734631377218
1106	34-35	0.5007918780051384
1106	36-37	0.4621759787225912
1106	38-39	0.6086620241133573
1106	40-41	0.6058201492166972
1106	42-43	0.6243510378303796
1106	44-45	0.6320558219490522
1106	46-47	0.5952252743796222
1106	48-49	0.5380257449188122
1106	50-51	0.5742432901591528
1106	52-53	0.5315388179803548
1106	54-55	0.5036247506930778
1106	56-57	0.5436597503739762
1106	58-59	0.44450033131831646
1106	60-61	0.477633830421496
1106	62-63	0.4167115203855758
1106	64-65	0.5103919301636779
1106	66-67	0.599887204478307
1106	68-69	0.4535398836689808
1106	70-71	0.43870777388545434
1106	72-73	0.37992129947195963
1106	74-75	0.4078102905008478
1106	76-77	0.4014539262248604
1106	78-79	0.4372073683194486
1106	80-81	0.45807164252537547
1106	82-83	0.4414230753723025
1106	84-85	0.42850460900136866
1106	86-87	0.5062763500759573
1106	88-89	0.5200597336516459
1106	90-91	0.45734785735543326
1106	92-93	0.4822217608263166
1106	94-95	0.5325738229208703
1106	96-97	0.5349583863671157
1106	98-99	0.5427741032999016
1106	100	0.5672507082834066
1107	1	0.496244386726012
1107	2	0.5992616250636935
1107	3	0.4653866923804415
1107	4	0.6783612408981909
1107	5	0.5983838577427463
1107	6	0.36615765968173264
1107	7	0.3654419299695846
1107	8	0.4340916868378599
1107	9	0.529832470143333
1107	10-11	0.4889774123792563
1107	12-13	0.3647226695864134
1107	14-15	0.5466709901492806
1107	16-17	0.6932769520509581
1107	18-19	0.8637253390072388
1107	20-21	0.5683727559504632
1107	22-23	0.7939656189768556
1107	24-25	0.5510989126826829
1107	26-27	0.5194534293976929
1107	28-29	0.5945078682438378
1107	30-31	0.618815410898442
1107	32-33	0.5588917700447809
1107	34-35	0.4343005239577451
1107	36-37	0.48047745173673917
1107	38-39	0.4241851479125245
1107	40-41	0.32888514426783644
1107	42-43	0.4159144900010858
1107	44-45	0.3863987877727979
1107	46-47	0.3190902383790828
1107	48-49	0.33904171712755016
1107	50-51	0.43681786271335454
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2216	82-83	-1.5660478244681748
2216	84-85	-1.4109678699844181
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2216	88-89	-1.3728595948082862
2216	90-91	-1.2812199705318612
2216	92-93	-1.2501018581439638
2216	94-95	-1.169803907138835
2216	96-97	-1.1315886646164515
2216	98-99	-1.1383030438522823
2216	100	-1.0802915337495094
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	15964.0
1	2518.0
2	419.0
3	410.0
4	366.0
5	220.0
6	212.0
7	242.0
8	470.0
9	877.0
10	1457.0
11	2688.0
12	4505.0
13	6395.0
14	8491.0
15	10537.0
16	12758.0
17	15252.0
18	17668.0
19	20083.0
20	23076.0
21	26986.0
22	30528.0
23	35228.0
24	40051.0
25	44914.0
26	50971.0
27	58319.0
28	69110.0
29	82483.0
30	97299.0
31	117756.0
32	144704.0
33	184562.0
34	255695.0
35	345736.0
36	508424.0
37	771366.0
38	626326.0
39	210871.0
40	190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20239287992556	21.321775039242716	12.794267845291913	23.681564235539813
2	15.315258846263438	19.879794188093655	41.163164777236894	23.64178218840601
3	18.48999542022266	26.991608792440992	28.98400787732153	25.53438791001482
4	12.095672862422457	16.872524301162002	40.38237694255177	30.649425893863768
5	13.02192477718388	38.75784925615674	34.98783001345083	13.23239595320855
6	29.40841731998326	38.71051686865789	18.40393228395091	13.477133527407945
7	26.751321883024133	31.87950154936136	23.90168859221614	17.46748797539837
8	26.00741905094162	35.19954215648997	20.132466252987285	18.660572539581118
9	26.080927018902205	15.294447577881892	21.809648118168983	36.814977285046915
10-11	25.6623244702757	25.938673386660938	27.502520193899073	20.896481949164293
12-13	25.76342542395388	23.831676273743664	27.562739632355477	22.84215866994698
14-15	23.366977571684	24.95285585254615	25.036198628431716	26.643967947338137
16-17	22.153172308260146	27.467381365515347	26.455258740257015	23.92418758596749
18-19	21.572976347795585	28.180403915541675	28.030284712843766	22.216335023818974
20-21	23.420266360176853	27.33306941542095	26.829342566016617	22.41732165838558
22-23	23.907088225972036	26.88918194566999	26.578162589725412	22.62556723863256
24-25	23.329737133238233	27.01277315383263	27.279527993836577	22.377961719092553
26-27	23.102232219098852	27.346797495432217	26.276218771139426	23.274751514329502
28-29	22.181659424253645	27.78252271187096	27.026832428064374	23.008985435811017
30-31	23.054880675094193	27.326542944002806	26.89896377390366	22.719612606999334
32-33	23.471049669592915	27.619894182438653	25.87440231451576	23.034653833452666
34-35	23.455904927169215	27.457641118832022	26.851450923766627	22.235003030232136
36-37	23.160353113481865	27.02713972955435	26.446909315843936	23.365597841119847
38-39	23.348618582982464	27.065779727453492	27.08026200372226	22.505339685841786
40-41	22.870253745319822	26.211016806032877	27.79691851926213	23.121810929385173
42-43	22.81071479221415	26.481995727101793	27.122433761254918	23.584855719429136
44-45	22.989745503649434	27.39824427126669	26.916114964871703	22.695895260212176
46-47	23.00568935616013	27.120541068581428	26.370243580993684	23.50352599426476
48-49	23.711254789284546	26.64984138741643	26.73925617663825	22.899647646660775
50-51	23.327304825816565	26.627308184911236	26.633163727371695	23.412223261900508
52-53	22.75038472974165	27.197618759781612	26.459382014578885	23.59261449589785
54-55	22.7047013261974	27.05739143664654	26.49958415412191	23.738323083034153
56-57	23.825016290629534	26.502586766389108	26.236513018519698	23.435883924461663
58-59	22.674126279201957	26.925300317324556	27.194253104465115	23.20632029900837
60-61	22.75857553375402	27.53482515932507	26.474413629893256	23.232185677027655
62-63	23.17086063670841	26.873460315726184	26.045221093140388	23.91045795442502
64-65	22.324481769462366	27.061982730092478	26.522827446783705	24.09070805366145
66-67	23.189776200492407	26.598746216706427	26.414989043837977	23.796488538963185
68-69	22.49348318766341	26.783003907669045	26.672217117532952	24.05129578713459
70-71	22.4784305750226	26.436785364943223	26.933824486597235	24.150959573436946
72-73	23.126570270067496	26.55212806476329	27.1870589486997	23.134242716469515
74-75	23.330793370304388	26.94398009156937	26.70578993939378	23.019436598732458
76-77	22.96378783633459	26.91122366407106	26.368456791930477	23.756531707663868
78-79	22.848737964444275	26.87149672415357	26.471620965521716	23.80814434588044
80-81	22.422998789267766	27.217153399146422	27.09677284446796	23.26307496711785
82-83	23.338820694758507	26.239027999919497	28.01186482898435	22.41028647633765
84-85	23.564735012518867	26.747635426747244	26.612042684919274	23.075586875814615
86-87	22.222292146109634	27.449906854826605	27.155898759475942	23.17190223958782
88-89	23.314132914749745	26.15327074299244	26.96756305868387	23.565033283573946
90-91	22.981496033700076	26.260499396504063	27.16466539824291	23.593339171552955
92-93	23.15472884643318	26.560384257180996	27.08161324393798	23.203273652447844
94-95	22.07636301253261	26.548099732415924	27.695852019632035	23.679685235419427
96-97	22.497834376819746	26.45443778581954	27.17886667253806	23.868861164822654
98-99	21.870459057830285	27.199089363441647	27.072588696040217	23.85786288268785
100	22.70435114746626	26.4580606543979	26.298416146676317	24.539172051459527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13388.0
1	7891.0
2	2162.0
3	1520.5
4	856.0
5	517.0
6	407.5
7	368.5
8	366.5
9	374.0
10	352.0
11	333.0
12	338.0
13	346.5
14	374.0
15	463.5
16	536.5
17	613.0
18	763.0
19	942.0
20	1142.5
21	1434.0
22	1984.5
23	2781.0
24	3720.0
25	4949.5
26	7011.0
27	9411.0
28	12838.5
29	19750.0
30	29348.5
31	39210.5
32	52534.5
33	65734.0
34	76315.5
35	87834.0
36	99220.0
37	118765.0
38	127580.0
39	118842.5
40	110096.0
41	102450.5
42	100747.0
43	106940.0
44	118005.0
45	139313.0
46	160193.0
47	173908.5
48	181829.5
49	176694.0
50	167966.5
51	172615.5
52	175623.5
53	168344.5
54	159241.0
55	137881.0
56	119867.5
57	98849.5
58	78123.5
59	73990.5
60	61835.5
61	44051.0
62	33001.5
63	26847.0
64	19549.0
65	10145.5
66	5830.5
67	4011.0
68	2912.0
69	2161.0
70	1613.5
71	1210.5
72	926.5
73	750.0
74	569.0
75	403.5
76	310.0
77	211.0
78	117.0
79	75.5
80	51.5
81	40.0
82	33.5
83	24.0
84	22.5
85	20.0
86	13.0
87	7.5
88	8.0
89	7.0
90	7.0
91	9.0
92	8.0
93	6.5
94	5.5
95	6.5
96	5.0
97	5.0
98	5.0
99	1.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9987709714213805
2	0.4470471203889003
3	0.47918334469974605
4	0.46579325123689364
5	0.43222701694457827
6	0.4870613996885698
7	0.5041175187402808
8	0.5067955374328512
9	0.5203416319845913
10-11	0.5337447255381843
12-13	0.5323537158289365
14-15	0.5293766950493315
16-17	0.5343817299844753
18-19	0.5330167204567088
20-21	0.5315087099307952
22-23	0.49488745431443115
24-25	0.5285706894234122
26-27	0.5095645567606062
28-29	0.5154275976846318
30-31	0.5183136178290524
32-33	0.5203546320753318
34-35	0.5333287226344839
36-37	0.5507098439547108
38-39	0.5398027678233194
40-41	0.5447818025769819
42-43	0.5422207847010773
44-45	0.5517108509417396
46-47	0.5506188433195264
48-49	0.5235916546697496
50-51	0.5379827551196308
52-53	0.5301307003122882
54-55	0.5405827732677574
56-57	0.528011685521565
58-59	0.5342647291678095
60-61	0.527829684251196
62-63	0.5215246402419889
64-65	0.5333417227252246
66-67	0.5310797069363544
68-69	0.5515288496713707
70-71	0.5349017336141005
72-73	0.5395037657362848
74-75	0.53591574069187
76-77	0.5245536613845565
78-79	0.5541678680917193
80-81	0.5289216918734092
82-83	0.5295196960474784
84-85	0.5382297568437028
86-87	0.843874890246734
88-89	1.1134447718445075
90-91	1.1038637049686606
92-93	1.1030446992520009
94-95	1.1088947400852858
96-97	1.1016796897242342
98-99	1.0970646575113094
100	1.0933076312872663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3846127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.989708573662377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.33892311026273	22.294026397566743
2	12.634046979297162	7.577827740301628
3	4.926128159004346	4.4319944365515695
4	2.4268229120155747	2.9111884756493516
5	1.3584002954926522	2.036901449410075
6	0.8669169776348028	1.5599152510096757
7	0.5911575996668275	1.241005089457975
8	0.4176934284341126	1.0021203353498354
9	0.3066290404919396	0.827614400811749
>10	1.6247478561963722	9.148843305171823
>50	0.19697624808596675	4.152820692023307
>100	0.23724478567626636	15.648528399945208
>500	0.04500378461818334	9.440271347050743
>1k	0.028094848200938903	14.723359458788444
>5k	8.671249444734229E-4	1.747316159855934
>10k+	3.4684997778936915E-4	1.2562670610560827
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13556	0.35245846016005195	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	11886	0.3090381570863365	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11742	0.30529413095303404	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11125	0.2892520189790925	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	9511	0.24728772606832797	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	8519	0.22149554603891133	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	8462	0.22001353569447915	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	6830	0.17758123951705185	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6489	0.16871517763193988	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5928	0.15412907582094923	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5602	0.14565301665805627	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5471	0.14224699288401033	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5319	0.13829496529885776	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5061	0.131586918476691	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4907	0.12758289052857588	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	4904	0.1275048899841321	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4777	0.12420286693601121	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4728	0.12292885804342914	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4701	0.12222685314343493	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4662	0.12121284606566554	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4475	0.11635081212866866	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4457	0.11588280886200586	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4442	0.11549280613978685	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4184	0.10878475931762002	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4171	0.10844675695836356	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4155	0.10803075405466331	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	3966	0.10311671975470388	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	3909	0.10163470941027168	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	3896	0.10129670705101522	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.200036296253348E-5
2	0.0	0.0	0.0	0.0	5.200036296253348E-5
3	0.0	0.0	0.0	0.0	5.200036296253348E-5
4	0.0	0.0	0.0	0.0	5.200036296253348E-5
5	0.0	0.0	0.0	0.0	7.800054444380022E-5
6	0.0	0.0	0.0	0.0	7.800054444380022E-5
7	0.0	0.0	0.0	0.0	7.800054444380022E-5
8	0.0	0.0	0.0	0.0	7.800054444380022E-5
9	0.0	0.0	0.0	0.0	7.800054444380022E-5
10-11	0.0	0.0	0.0	0.0	7.800054444380022E-5
12-13	0.0	0.0	0.0	0.0	2.3400163333140068E-4
14-15	0.0	0.0	0.0	0.0	2.730019055533008E-4
16-17	0.0	0.0	0.0	0.0	2.8600199629393415E-4
18-19	0.0	0.0	0.0	0.0	2.8600199629393415E-4
20-21	0.0	0.0	0.0	3.900027222190011E-5	3.120021777752009E-4
22-23	0.0	0.0	0.0	1.1700081666570034E-4	4.1600290370026784E-4
24-25	0.0	0.0	0.0	2.600018148126674E-4	4.1600290370026784E-4
26-27	0.0	0.0	0.0	4.810033574034347E-4	4.4200308518153457E-4
28-29	0.0	0.0	0.0	0.0011180078036944698	4.4200308518153457E-4
30-31	0.0	0.0	0.0	0.004667032575887379	4.5500317592216794E-4
32-33	0.0	0.0	0.0	0.01388409691099644	4.680032666628013E-4
34-35	0.0	0.0	0.0	0.027183189738664376	5.200036296253348E-4
36-37	0.0	0.0	0.0	0.05271536795326831	5.200036296253348E-4
38-39	0.0	0.0	0.0	0.09694167665290304	5.200036296253348E-4
40-41	0.0	0.0	0.0	0.14688802527841643	5.200036296253348E-4
42-43	0.0	0.0	0.0	0.2003313983131602	5.330037203659683E-4
44-45	0.0	0.0	0.0	0.26422684430337323	5.850040833285017E-4
46-47	0.0	0.0	0.0	0.3453214103434442	6.370044462910352E-4
48-49	0.0	0.0	0.0	0.44499310605188025	7.150049907348353E-4
50-51	0.0	0.0	0.0	0.5571448888713243	7.540052629567355E-4
52-53	0.0	0.0	0.0	0.6656436461926505	7.670053536973688E-4
54-55	0.0	0.0	0.0	0.7852444810064774	7.800054444380022E-4
56-57	0.0	0.0	0.0	0.9332245139071071	7.930055351786356E-4
58-59	0.0	0.0	0.0	1.1168507956185534	8.060056259192689E-4
60-61	0.0	0.0	0.0	1.3101231446595496	8.060056259192689E-4
62-63	0.0	0.0	0.0	1.5140945683800873	8.840061703630691E-4
64-65	0.0	0.0	0.0	1.7276080587042497	9.100063518443359E-4
66-67	0.0	0.0	0.0	1.9727247696188919	9.100063518443359E-4
68-69	0.0	0.0	0.0	2.2563087490350684	9.100063518443359E-4
70-71	0.0	0.0	0.0	2.5788020000379603	9.100063518443359E-4
72-73	0.0	0.0	0.0	2.914620344050001	0.0010140070777694028
74-75	0.0	0.0	0.0	3.247032664287997	0.00115700807591637
76-77	0.0	0.0	0.0	3.6238792946774767	0.00119600834813827
78-79	0.0	1.300009074063337E-5	0.0	4.035202165711117	0.0012090084388789034
80-81	0.0	2.600018148126674E-5	0.0	4.486032312505541	0.0012220085296195367
82-83	0.0	2.600018148126674E-5	0.0	4.960418623721994	0.001300009074063337
84-85	0.0	2.600018148126674E-5	0.0	5.425470869786671	0.001300009074063337
86-87	0.0	2.600018148126674E-5	0.0	5.925116357312174	0.0013520094370258705
88	0.0	2.600018148126674E-5	0.0	6.316172086881166	0.001404009799988404
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	3985	0.0	35.754517	1
GGGCACC	3990	0.0	35.140648	94
CAACGCA	7705	0.0	32.136364	5
GTATCAA	8530	0.0	31.803219	1
TAGGCAT	5230	0.0	31.353245	5
GGTATCA	5595	0.0	30.694006	1
ATCAACG	8195	0.0	30.10215	3
GCATCGT	4465	0.0	29.757355	8
TACCCCG	215	1.8189894E-12	28.409504	5
CTAGGCA	5890	0.0	28.238092	4
TCTAACG	2080	0.0	28.012823	2
CTAACGC	2105	0.0	27.232964	3
CGCCTAC	2115	0.0	26.637913	7
TATCAAC	10065	0.0	26.610903	2
AACGCAG	9980	0.0	26.060345	6
CTGTGCG	5770	0.0	25.794996	9
ACGCAGA	9535	0.0	25.604246	7
TAACGCC	2230	0.0	25.493408	4
GTACATG	16485	0.0	25.35695	1
TATTCTC	2285	0.0	25.291677	5
>>END_MODULE