##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3846127
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83328865635482	31.0	31.0	34.0	30.0	34.0
2	31.833552818198672	31.0	31.0	34.0	30.0	34.0
3	32.01130487890806	33.0	31.0	34.0	30.0	34.0
4	35.73827099313153	37.0	35.0	37.0	35.0	37.0
5	35.64233708351284	37.0	35.0	37.0	33.0	37.0
6	35.67886317846499	37.0	35.0	37.0	33.0	37.0
7	35.597131087974994	37.0	35.0	37.0	33.0	37.0
8	35.58413567726703	37.0	35.0	37.0	33.0	37.0
9	37.184337646676774	39.0	37.0	39.0	33.0	39.0
10-11	37.131067564851605	39.0	37.0	39.0	33.0	39.0
12-13	37.05741490075601	39.0	37.0	39.0	33.0	39.0
14-15	38.243195427504084	40.0	38.0	41.0	33.0	41.0
16-17	38.249265559873606	40.0	37.5	41.0	33.0	41.0
18-19	38.311043161081265	40.0	38.0	41.0	33.0	41.0
20-21	38.32678263094276	40.0	38.0	41.0	33.0	41.0
22-23	38.29574894432763	40.0	38.0	41.0	33.0	41.0
24-25	38.223629380933076	40.0	37.0	41.0	33.0	41.0
26-27	38.07394750615359	40.0	37.0	41.0	33.0	41.0
28-29	38.00964632733137	40.0	37.0	41.0	33.0	41.0
30-31	37.88476940569045	40.0	37.0	41.0	33.0	41.0
32-33	37.75552484356341	40.0	36.5	41.0	33.0	41.0
34-35	37.658341365222725	40.0	36.0	41.0	32.0	41.0
36-37	37.5727295276521	40.0	36.0	41.0	32.0	41.0
38-39	37.45556139981857	40.0	36.0	41.0	32.0	41.0
40-41	37.32659633964245	40.0	36.0	41.0	31.0	41.0
42-43	37.19949328246311	39.0	35.5	41.0	31.0	41.0
44-45	36.99398745803246	39.0	35.0	41.0	31.0	41.0
46-47	36.74270714409586	39.0	35.0	41.0	30.0	41.0
48-49	36.46721390115303	39.0	35.0	41.0	30.0	41.0
50-51	36.25821105231314	39.0	35.0	41.0	29.0	41.0
52-53	36.06261051702141	38.0	35.0	40.5	29.0	41.0
54-55	35.8594049286464	38.0	35.0	40.0	29.0	41.0
56-57	35.59366214376176	37.5	34.0	40.0	28.0	41.0
58-59	35.305997695863915	37.0	34.0	40.0	28.0	41.0
60-61	35.04777039343735	36.5	34.0	40.0	28.0	41.0
62-63	34.767136264611125	36.0	34.0	39.5	27.0	41.0
64-65	34.4908689962656	35.5	33.5	39.0	27.0	41.0
66-67	34.269605891849125	35.0	33.0	39.0	26.5	41.0
68-69	34.08053010210011	35.0	33.0	38.5	26.0	41.0
70-71	33.87923422705491	35.0	33.0	37.5	26.0	40.0
72-73	33.679182460693575	35.0	33.0	37.0	26.0	40.0
74-75	33.49863304045862	35.0	33.0	37.0	26.0	39.5
76-77	33.343374776755944	35.0	33.0	36.0	26.5	39.0
78-79	33.2306191397216	35.0	33.0	36.0	27.0	39.0
80-81	33.08497470312343	35.0	33.0	36.0	27.0	38.0
82-83	32.896636148520315	35.0	33.0	35.0	26.5	37.0
84-85	32.75395703262009	35.0	33.0	35.0	26.0	37.0
86-87	32.62281211722858	35.0	33.0	35.0	26.0	36.5
88-89	32.5142825496922	35.0	33.0	35.0	26.0	36.0
90-91	32.42244834868947	35.0	33.0	35.0	26.0	36.0
92-93	32.345474291410554	35.0	33.0	35.0	26.0	36.0
94-95	32.30424671364206	35.0	33.0	35.0	26.0	35.0
96-97	32.250482628368744	35.0	33.0	35.0	26.0	35.0
98-99	32.21126772464872	35.0	33.0	35.0	25.0	35.0
100	31.71036993838217	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47033948097275413
1101	2	0.6409888718827972
1101	3	0.5146096997917198
1101	4	0.3396353630940325
1101	5	0.3792437256639971
1101	6	0.39678952506636733
1101	7	0.4146052078446516
1101	8	0.4559920011176928
1101	9	0.5477806325321879
1101	10-11	0.5430877084123154
1101	12-13	0.5364955588059743
1101	14-15	0.706171967023451
1101	16-17	0.7662274065392012
1101	18-19	0.6769448454782179
1101	20-21	0.6462957567554426
1101	22-23	0.6631243411543792
1101	24-25	0.7159596627859273
1101	26-27	0.6728904458758649
1101	28-29	0.8009316031772471
1101	30-31	0.8307866677687343
1101	32-33	0.839147978230784
1101	34-35	0.8000064414717798
1101	36-37	0.7674696627069437
1101	38-39	0.7839208954287571
1101	40-41	0.8246514214299907
1101	42-43	0.8409689476301381
1101	44-45	0.777060362925603
1101	46-47	0.7890234235576798
1101	48-49	0.8939773767135719
1101	50-51	0.8624611600054521
1101	52-53	0.9071005838426487
1101	54-55	0.8254896190552188
1101	56-57	0.8764978905358163
1101	58-59	0.8854974368162658
1101	60-61	0.8908438914909951
1101	62-63	0.8236357272405215
1101	64-65	0.8838646926542921
1101	66-67	0.87725166608449
1101	68-69	0.8330838915216034
1101	70-71	0.817809006774155
1101	72-73	0.7549127600307131
1101	74-75	0.7121372808428816
1101	76-77	0.7326754685764172
1101	78-79	0.7483837872756638
1101	80-81	0.7567261150918583
1101	82-83	0.7601198886938434
1101	84-85	0.7447593450259546
1101	86-87	0.7432781449078121
1101	88-89	0.7473819468102718
1101	90-91	0.6979261986826302
1101	92-93	0.7086410101837046
1101	94-95	0.7117411595746859
1101	96-97	0.7435158009400666
1101	98-99	0.717523950819654
1101	100	0.7261831330796937
1102	1	0.40082780414476815
1102	2	0.5231636153822663
1102	3	0.4269195501209424
1102	4	0.3092276427639078
1102	5	0.32933104151100423
1102	6	0.3320959790126352
1102	7	0.35994011955284577
1102	8	0.35159427521249853
1102	9	0.44340474728387846
1102	10-11	0.49330103929434443
1102	12-13	0.46199105518650185
1102	14-15	0.5731527327434236
1102	16-17	0.6017346600347793
1102	18-19	0.6520681618617985
1102	20-21	0.600617265492815
1102	22-23	0.5695922250970469
1102	24-25	0.6375365155256318
1102	26-27	0.6978663626776438
1102	28-29	0.7059790550012011
1102	30-31	0.7468202039073262
1102	32-33	0.684160668617281
1102	34-35	0.7066435539580098
1102	36-37	0.7353261802922688
1102	38-39	0.7083555264888943
1102	40-41	0.6250499067000277
1102	42-43	0.6455025972699815
1102	44-45	0.6463837656894142
1102	46-47	0.6580888755914387
1102	48-49	0.6817056057304285
1102	50-51	0.6797107061466647
1102	52-53	0.6383878481842942
1102	54-55	0.5975976266294509
1102	56-57	0.6305119810633784
1102	58-59	0.5468422341846733
1102	60-61	0.5358466000833033
1102	62-63	0.5419808089175646
1102	64-65	0.5418888038038787
1102	66-67	0.5411313684480845
1102	68-69	0.5154145632770621
1102	70-71	0.4812209477600575
1102	72-73	0.49836082610633525
1102	74-75	0.4988912177455518
1102	76-77	0.4501675512652028
1102	78-79	0.4299288093777136
1102	80-81	0.4115661099132524
1102	82-83	0.48340839985339557
1102	84-85	0.4632340233718466
1102	86-87	0.44594663461147377
1102	88-89	0.44450441541381736
1102	90-91	0.46064773808463855
1102	92-93	0.4989486880254219
1102	94-95	0.4447572712547654
1102	96-97	0.45392480071750185
1102	98-99	0.46828751129464763
1102	100	0.4699006549703455
1103	1	0.2809999414148052
1103	2	-0.15608453227472197
1103	3	0.19334717187913242
1103	4	0.1917812748523673
1103	5	0.22926003077834878
1103	6	0.254736104780406
1103	7	0.32177340924829423
1103	8	0.32660150096542395
1103	9	0.3982113181285598
1103	10-11	0.46025667940537573
1103	12-13	0.4654109048681718
1103	14-15	0.4959825132732263
1103	16-17	0.4506257478194655
1103	18-19	0.4730041433910728
1103	20-21	0.4568811148525853
1103	22-23	0.4394050412591568
1103	24-25	0.4532097970148996
1103	26-27	0.5820961427540539
1103	28-29	0.5764891138159953
1103	30-31	0.595076700567617
1103	32-33	0.60847838752359
1103	34-35	0.5550315309852181
1103	36-37	0.5988325576567917
1103	38-39	0.5585857871487221
1103	40-41	0.6121471205630513
1103	42-43	0.6182491257915856
1103	44-45	0.5873389932241295
1103	46-47	0.6129885878081254
1103	48-49	0.673257708792903
1103	50-51	0.7151144018784237
1103	52-53	0.6453531906396464
1103	54-55	0.6274673590555722
1103	56-57	0.6360513849359322
1103	58-59	0.5915622206739357
1103	60-61	0.5760179609391045
1103	62-63	0.6207731390783664
1103	64-65	0.5658850475458124
1103	66-67	0.6311357719069264
1103	68-69	0.5628227282078413
1103	70-71	0.5236759582388189
1103	72-73	0.4909517940851913
1103	74-75	0.5418059561279591
1103	76-77	0.5064653191592825
1103	78-79	0.5133674686860132
1103	80-81	0.4751967702280595
1103	82-83	0.5186466400113474
1103	84-85	0.46799341430038055
1103	86-87	0.4595516222511833
1103	88-89	0.4940779826461892
1103	90-91	0.3983957230913404
1103	92-93	0.4137890243884641
1103	94-95	0.4794378533047521
1103	96-97	0.46669641818670726
1103	98-99	0.496522155011192
1103	100	0.4806219721315941
1104	1	0.35260459055716353
1104	2	0.4404873339693083
1104	3	0.33208465909794427
1104	4	0.2640143851583616
1104	5	0.2884586955780435
1104	6	0.28978688522114027
1104	7	0.3010307621916084
1104	8	0.3292190189026698
1104	9	0.3342605931565217
1104	10-11	0.36109382372945475
1104	12-13	0.3973827369999796
1104	14-15	0.5071805353450571
1104	16-17	0.4762666523920984
1104	18-19	0.44759385957093656
1104	20-21	0.451510850430509
1104	22-23	0.4618665059174063
1104	24-25	0.48506184408280006
1104	26-27	0.5715356765032311
1104	28-29	0.49823403279363987
1104	30-31	0.5243557150670526
1104	32-33	0.5341245222506217
1104	34-35	0.534829115293121
1104	36-37	0.542078699473592
1104	38-39	0.5267167856636519
1104	40-41	0.5785555822555111
1104	42-43	0.5286577577546367
1104	44-45	0.518533377433009
1104	46-47	0.5706948280506126
1104	48-49	0.5151595120629651
1104	50-51	0.4942196154416152
1104	52-53	0.5222033940781259
1104	54-55	0.505670530795534
1104	56-57	0.4537288709518066
1104	58-59	0.48457833739470857
1104	60-61	0.5173984630978552
1104	62-63	0.4752285042143285
1104	64-65	0.37218026477300725
1104	66-67	0.36690081709981825
1104	68-69	0.43779915619253984
1104	70-71	0.4161878154346752
1104	72-73	0.3832775013691432
1104	74-75	0.32148722761336046
1104	76-77	0.3410915563450416
1104	78-79	0.3429227515632647
1104	80-81	0.34384286631006233
1104	82-83	0.3765439406996123
1104	84-85	0.357897748965172
1104	86-87	0.40676421146527275
1104	88-89	0.3443256014004774
1104	90-91	0.33319114124363836
1104	92-93	0.406501908379866
1104	94-95	0.3567869698880912
1104	96-97	0.4107427300416404
1104	98-99	0.38351545837960543
1104	100	0.5136005654002105
1105	1	0.15178678500944898
1105	2	-0.38878946827188443
1105	3	0.0560345888570879
1105	4	0.11715050886218137
1105	5	0.17939094728485827
1105	6	0.23033060159682606
1105	7	0.24381434229596977
1105	8	0.21171459855712982
1105	9	0.29554395293731517
1105	10-11	0.30262501884512005
1105	12-13	0.2993587304261567
1105	14-15	0.37441348228278315
1105	16-17	0.37755448154089777
1105	18-19	0.3926628110416672
1105	20-21	0.38698435000114273
1105	22-23	0.4490457464430335
1105	24-25	0.46081219288219444
1105	26-27	0.3949532778955387
1105	28-29	0.4199510914611224
1105	30-31	0.4672458496401646
1105	32-33	0.43843990423914647
1105	34-35	0.4390140790516526
1105	36-37	0.4636745894309442
1105	38-39	0.4618610023273604
1105	40-41	0.4353337968983908
1105	42-43	0.4473646526797168
1105	44-45	0.473720748656568
1105	46-47	0.5172126243118171
1105	48-49	0.4124023411877076
1105	50-51	0.4509503615485855
1105	52-53	0.46833363545729867
1105	54-55	0.4694773445838365
1105	56-57	0.5240683246307682
1105	58-59	0.5184130810331666
1105	60-61	0.4664232492212008
1105	62-63	0.462096775882344
1105	64-65	0.44175237828459757
1105	66-67	0.4984756070524696
1105	68-69	0.515294038433197
1105	70-71	0.5114704493131583
1105	72-73	0.49017013257265774
1105	74-75	0.39125247109956973
1105	76-77	0.3775798022401986
1105	78-79	0.33306276339525454
1105	80-81	0.38950719087497987
1105	82-83	0.3767958976205321
1105	84-85	0.3632850393109379
1105	86-87	0.36153461433365663
1105	88-89	0.3700947637232588
1105	90-91	0.28547157336640083
1105	92-93	0.3012910248768321
1105	94-95	0.3229472315089055
1105	96-97	0.3282240635856155
1105	98-99	0.3090812422597651
1105	100	0.3184689946800461
1106	1	0.2697102416232724
1106	2	0.296086756233521
1106	3	0.25176178251528114
1106	4	0.18611309982734525
1106	5	0.12948861704507664
1106	6	0.18242325802987835
1106	7	0.17278709688378768
1106	8	0.1657510161680662
1106	9	0.20598516217168594
1106	10-11	0.2273828042808148
1106	12-13	0.26471185355458005
1106	14-15	0.32271181868460275
1106	16-17	0.26577718973399556
1106	18-19	0.31977217975803995
1106	20-21	0.271423349355004
1106	22-23	0.2516951642753327
1106	24-25	0.26387525452184946
1106	26-27	0.28677575383703413
1106	28-29	0.25622544992717877
1106	30-31	0.3061063111166433
1106	32-33	0.3416840415349185
1106	34-35	0.3151596955448426
1106	36-37	0.3690661236980972
1106	38-39	0.2561822309376893
1106	40-41	0.25191382430637077
1106	42-43	0.1994020841205426
1106	44-45	0.2712294224458489
1106	46-47	0.3068288146070657
1106	48-49	0.20338703117823087
1106	50-51	0.20916109822514528
1106	52-53	0.26191616820761965
1106	54-55	0.2493083918360881
1106	56-57	0.30285688764305263
1106	58-59	0.2676551996976144
1106	60-61	0.2815897722674734
1106	62-63	0.3024320278916903
1106	64-65	0.23464592675142626
1106	66-67	0.2658209697798952
1106	68-69	0.24616709648184099
1106	70-71	0.2088923508024223
1106	72-73	0.2042364387872695
1106	74-75	0.22335639714151512
1106	76-77	0.1995200230509866
1106	78-79	0.27002336457611875
1106	80-81	0.26392901854008244
1106	82-83	0.20320242557080093
1106	84-85	0.2625508466701518
1106	86-87	0.1981003608113383
1106	88-89	0.22155256809119095
1106	90-91	0.17149128249257473
1106	92-93	0.1667248150850611
1106	94-95	0.22142797581507523
1106	96-97	0.22567136587378656
1106	98-99	0.2997150279757932
1106	100	0.31926856382436597
1107	1	-0.031663795289414765
1107	2	-0.571164199576053
1107	3	-0.16198130363373053
1107	4	-0.06827197983962918
1107	5	0.004805247161328907
1107	6	0.05838032908000912
1107	7	0.03539000409747928
1107	8	0.048697044463914096
1107	9	-0.0033625584408056852
1107	10-11	0.059676747262159324
1107	12-13	0.08285224597020147
1107	14-15	0.08894000973006655
1107	16-17	0.06134339774143882
1107	18-19	0.08752850168635717
1107	20-21	0.06813846958323211
1107	22-23	0.09447526014739793
1107	24-25	0.024256996133047437
1107	26-27	0.07287119894348848
1107	28-29	-0.004164830896463911
1107	30-31	0.07878548902337457
1107	32-33	0.13926919106817337
1107	34-35	0.11103971960989156
1107	36-37	0.09718380957152561
1107	38-39	0.11476433352468973
1107	40-41	0.07855228569554384
1107	42-43	0.12531839983851256
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2216	80-81	-0.6793253585675529
2216	82-83	-0.6679069593462899
2216	84-85	-0.705218768586775
2216	86-87	-0.6879198479243982
2216	88-89	-0.6663854934892335
2216	90-91	-0.7295353802407831
2216	92-93	-0.7526808506288418
2216	94-95	-0.7211918638713257
2216	96-97	-0.7358196863637829
2216	98-99	-0.7509495030195126
2216	100	-0.8496275853354334
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	44.0
14	194.0
15	666.0
16	1726.0
17	3538.0
18	6171.0
19	9667.0
20	13826.0
21	20017.0
22	27639.0
23	32446.0
24	34430.0
25	35619.0
26	39261.0
27	47347.0
28	59257.0
29	73977.0
30	91229.0
31	113168.0
32	140142.0
33	177622.0
34	243827.0
35	336478.0
36	498895.0
37	848325.0
38	717910.0
39	272064.0
40	632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.08019018059043	21.74129552466086	14.030239171543228	24.148275123205483
2	14.913063250577164	20.3523856350968	40.350295128104655	24.384255986221376
3	17.96634513256619	26.67972078300777	30.011822546873525	25.342111537552515
4	12.304025217099564	17.42848523150785	39.056257330930215	31.211232220462364
5	12.578668844498491	38.77916093672769	35.09910356843569	13.543066650338128
6	29.304596546083893	39.13417315652863	17.7568499428126	13.804380354574874
7	25.800248458315632	32.620346021624925	23.895990710633424	17.683414809426022
8	26.0979421636363	35.102507015498965	19.655487195300623	19.144063625564105
9	25.936533037000597	14.7076006590526	21.712699554642892	37.64316674930391
10-11	25.654028065115895	26.029834688246122	27.212127940653026	21.104009305984956
12-13	26.013974577542555	23.864721575756597	27.804945078516646	22.3163587681842
14-15	23.361724611535443	24.76524930430274	25.725366128397294	26.147659955764524
16-17	21.682171111031376	27.247624411270532	27.07129808314834	23.998906394549756
18-19	21.09771726206649	27.125924338951883	29.8310872209888	21.945271177992822
20-21	22.732773540540993	26.302079846328468	29.078783310633	21.88636330249754
22-23	23.13736089162482	25.878577843229326	28.771402838510262	22.21265842663559
24-25	22.60674179505773	26.012011563840716	29.51324800247106	21.8679986386305
26-27	22.449534882148825	26.30036176699543	28.53072523440494	22.7193781164508
28-29	21.561222497333034	26.791861007189834	29.25006116542693	22.3968553300502
30-31	22.53330428246389	26.34558089215463	28.9447670344739	22.17634779090758
32-33	22.961072268284433	26.593102619856285	28.167985612539574	22.277839499319704
34-35	22.83319139343724	26.480043545279436	28.933144553921537	21.753620507361788
36-37	22.64263504559262	26.04169077100158	28.55824833657339	22.75742584683241
38-39	22.752992815889854	26.045915800492285	29.22764900899008	21.973442374627776
40-41	22.31814592006864	25.35792959774318	29.766432989154634	22.55749149303355
42-43	22.458662340898876	25.542539916089012	28.85915977992769	23.139637963084418
44-45	22.705737486047653	26.448268608914887	28.434604473539228	22.411389431498232
46-47	22.738172712445532	26.315030678914137	27.663647092256703	23.283149516383624
48-49	23.628935585150547	26.067556336489204	27.698841569342104	22.60466650901814
50-51	23.072119043390924	26.233364108881478	27.2949515187616	23.399565328965995
52-53	22.454042729218248	26.67652420213893	27.263361298261863	23.606071770380957
54-55	22.512733305261712	26.64577458627402	27.09855621823011	23.742935890234158
56-57	23.567521202853317	26.088164401462617	26.882861084861727	23.461453310822343
58-59	22.39718229990753	26.53988160261958	27.790273069022497	23.272663028450395
60-61	22.60649230406828	27.057455000789105	27.017466680082773	23.318586015059843
62-63	23.018818671354328	26.37375208878958	26.62687685560045	23.980552384255642
64-65	22.15973367494105	26.505352007357015	27.12740634929632	24.20750796840562
66-67	22.85510852865753	26.148941519611807	27.02323662219162	23.97271332953904
68-69	22.34163856978589	26.047085757075244	27.38103270418421	24.230242968954652
70-71	22.307152499573593	25.724695899426326	27.583307852950107	24.38484374804997
72-73	22.96248927817516	25.769832873433458	27.79804203033337	23.46963581805801
74-75	23.06908300691125	26.27254561383233	27.25816241539037	23.40020896386605
76-77	22.758444869674964	26.273638569341255	26.77678227496826	24.191134286015526
78-79	22.522697614654184	26.292715573063578	27.048076380570595	24.136510431711635
80-81	22.11379355174207	26.772919916796674	27.57385595423817	23.53943057722309
82-83	22.995127091734517	25.745135834446703	28.51776512615961	22.74197194765917
84-85	23.36085485333145	26.163099165747834	26.98930380266047	23.486742178260247
86-87	21.92564434993476	26.8356191953121	27.518270514382653	23.72046594037049
88-89	22.86892632023996	25.626513735477594	27.371392695794437	24.133167248488004
90-91	22.483704690615337	25.637030369027524	27.655729802084927	24.223535138272215
92-93	22.740855498532028	26.02644300338475	27.294208900610826	23.9384925974724
94-95	21.589863012238325	25.90711019953207	27.95852705363015	24.544499734599455
96-97	22.009200878745546	25.774005138409706	27.540232718320656	24.676561264524093
98-99	21.40622475087749	26.49564182676276	27.429717168226773	24.668416254132975
100	22.23410561508528	25.74424301659841	26.65030401535508	25.371347352961227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	178.0
1	124.5
2	122.0
3	172.5
4	123.0
5	92.0
6	192.0
7	484.0
8	844.0
9	998.5
10	807.5
11	565.5
12	556.5
13	651.5
14	795.5
15	1116.0
16	1477.5
17	1901.0
18	2540.5
19	3359.5
20	4260.0
21	5163.5
22	6127.0
23	7172.0
24	8213.0
25	9276.5
26	11030.5
27	13126.5
28	16165.0
29	22517.0
30	31388.5
31	40085.0
32	52780.5
33	65621.0
34	74825.5
35	85597.5
36	96300.0
37	116439.0
38	125795.0
39	116067.0
40	107618.5
41	100165.0
42	97680.5
43	102929.5
44	113691.5
45	136587.5
46	158705.0
47	172943.0
48	181078.0
49	175623.5
50	167686.0
51	171353.5
52	173939.0
53	168226.5
54	161976.0
55	140379.0
56	121298.5
57	100980.5
58	79419.5
59	76300.0
60	64007.5
61	44471.0
62	32347.0
63	25894.5
64	18568.5
65	9036.5
66	5059.5
67	3512.5
68	2548.0
69	1928.0
70	1419.5
71	1044.5
72	742.5
73	618.5
74	493.0
75	347.0
76	252.5
77	139.5
78	73.0
79	39.0
80	18.0
81	9.0
82	5.5
83	2.5
84	2.5
85	3.0
86	1.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013494094188777438
2	0.004420030851815346
3	0.0022360156073889395
4	0.02904220271457495
5	0.002600018148126674
6	0.0
7	2.3400163333140065E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008970062611037026
16-17	0.0034580241370084763
18-19	0.0
20-21	3.900027222190011E-5
22-23	6.500045370316685E-4
24-25	0.0
26-27	1.300009074063337E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.0300281295963447E-4
36-37	0.0
38-39	0.0
40-41	3.7700263147836773E-4
42-43	7.150049907348353E-4
44-45	0.0
46-47	0.0
48-49	4.810033574034347E-4
50-51	0.0
52-53	0.0
54-55	3.2500226851583426E-4
56-57	1.4300099814696705E-4
58-59	2.9900208703456747E-4
60-61	1.0400072592506696E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015470107981353709
70-71	8.060056259192689E-4
72-73	0.0
74-75	1.9500136110950058E-4
76-77	0.0028210196907174413
78-79	4.680032666628013E-4
80-81	0.0033020230481208756
82-83	0.0017290120685042381
84-85	0.0059150412869881834
86-87	0.008138056803636488
88-89	0.007839054716601922
90-91	0.0061100426480976845
92-93	0.008996062792518291
94-95	0.0013910097092477707
96-97	0.004940034481440681
98-99	0.0020150140647981723
100	0.0839545860030103
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3846127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.257804716206724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.02992378498287	15.589688775333519
2	15.637518535012049	7.273887046667466
3	6.44526123459464	4.497078814174187
4	3.223210214526255	2.9985917491493765
5	1.8365489043768939	2.1357047884880602
6	1.1461091374904169	1.5993589501927339
7	0.7988036017828583	1.3004892723807795
8	0.5520929481714227	1.0272375979012631
9	0.400404585837521	0.8381278498394426
>10	2.219171780840434	9.683704605179717
>50	0.2762774662446844	4.546821737614169
>100	0.32591725920771225	16.841731435645528
>500	0.06393734105368346	10.444677591270468
>1k	0.04269941308130599	17.366131073675817
>5k	0.0016766785241350518	2.588961254587327
>10k+	4.471142731026805E-4	1.267807457900309
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	15042	0.3910947298412143	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12079	0.3140561921122209	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11599	0.3015761050012129	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10047	0.26122382334228694	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9855	0.25623178849788375	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9367	0.24354369993502553	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8380	0.21788152081301526	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7877	0.2048034295279381	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7578	0.19702937526503936	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6690	0.17394121410967447	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6391	0.16616715984677574	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6146	0.15979711538386537	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5960	0.15496108162834976	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5648	0.14684902500619454	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5364	0.13946497346551479	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5184	0.13478494079888675	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5073	0.13189892065446615	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5067	0.13174291956557857	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5006	0.1301569084952213	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4979	0.1294549035952271	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4826	0.1254768758285933	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4782	0.12433286784341756	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4747	0.12342286149157321	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4738	0.1231888598582418	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4673	0.12149884806195946	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4589	0.11931483281753306	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4588	0.1192888326360518	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4486	0.1166368141249626	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4393	0.11421879724720478	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4380	0.11388079488794832	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4377	0.11380279434350452	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4081	0.10610674062504957	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4048	0.10524873463616775	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	3975	0.10335072138803528	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3952	0.10275271721396616	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	1.300009074063337E-5
12-13	0.0	0.0	0.0	0.0	1.0400072592506697E-4
14-15	0.0	0.0	0.0	0.0	1.690011796282338E-4
16-17	0.0	0.0	0.0	0.0	2.3400163333140068E-4
18-19	0.0	0.0	0.0	2.600018148126674E-5	2.730019055533008E-4
20-21	0.0	0.0	0.0	2.600018148126674E-5	3.51002449997101E-4
22-23	0.0	0.0	0.0	9.100063518443359E-5	3.900027222190011E-4
24-25	0.0	0.0	0.0	2.3400163333140065E-4	3.900027222190011E-4
26-27	0.0	0.0	0.0	4.6800326666280136E-4	4.1600290370026784E-4
28-29	0.0	0.0	0.0	0.0011180078036944698	4.4200308518153457E-4
30-31	0.0	0.0	0.0	0.004732033029590546	4.4200308518153457E-4
32-33	0.0	0.0	0.0	0.014170098907290374	4.4200308518153457E-4
34-35	0.0	0.0	0.0	0.027612192733105275	4.4200308518153457E-4
36-37	0.0	0.0	0.0	0.05348237330696568	4.4200308518153457E-4
38-39	0.0	0.0	0.0	0.09816368518252258	4.4200308518153457E-4
40-41	0.0	0.0	0.0	0.148747038254327	4.4200308518153457E-4
42-43	0.0	0.0	0.0	0.2025414137390679	4.4200308518153457E-4
44-45	0.0	0.0	0.0	0.2672298652644596	4.6800326666280136E-4
46-47	0.0	0.0	0.0	0.34959844019711256	5.070035388847014E-4
48-49	0.0	0.0	0.0	0.4504141438907243	5.720039925878683E-4
50-51	0.0	0.0	0.0	0.5638269355120098	6.240043555504018E-4
52-53	0.0	0.0	0.0	0.6733266998203647	6.240043555504018E-4
54-55	0.0	0.0	0.0	0.7935645390804827	6.500045370316685E-4
56-57	0.0	0.0	0.0	0.9427145801477694	6.630046277723018E-4
58-59	0.0	0.0	0.0	1.1284338764684578	6.760047185129353E-4
60-61	0.0	0.0	0.0	1.3233962373057362	6.760047185129353E-4
62-63	0.0	0.0	0.0	1.528745670644781	7.02004899994202E-4
64-65	0.0	0.0	0.0	1.7444691763948512	7.02004899994202E-4
66-67	0.0	0.0	0.0	1.9924849075446547	7.410051722161021E-4
68-69	0.0	0.0	0.0	2.279149908466361	7.540052629567355E-4
70-71	0.0	0.0	0.0	2.6046071801581174	7.540052629567355E-4
72-73	0.0	0.0	0.0	2.943610546401614	8.060056259192689E-4
74-75	0.0	0.0	0.0	3.2804688976729057	8.580059888818024E-4
76-77	0.0	0.0	0.0	3.663646572253074	9.100063518443359E-4
78-79	0.0	0.0	0.0	4.083237500997757	9.100063518443359E-4
80-81	0.0	0.0	0.0	4.5429337096772935	9.100063518443359E-4
82-83	0.0	0.0	0.0	5.027356090945515	9.49006624066236E-4
84-85	0.0	0.0	0.0	5.50221040542863	9.620067148068694E-4
86-87	0.0	0.0	0.0	6.011345959194795	0.001053007349991303
88	0.0	0.0	0.0	6.409070735313732	0.0011440079851757366
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8475	0.0	45.55398	1
GGGCACC	3840	0.0	39.31454	94
GTATCAA	14410	0.0	38.115517	1
GTACTAG	4215	0.0	32.576782	1
TACCCCG	235	0.0	31.998688	5
CAACGCA	16415	0.0	31.952394	5
ATCAACG	16730	0.0	31.361786	3
TAGGCAT	5330	0.0	30.861776	5
GTGGTAT	2215	0.0	30.783443	1
TATCAAC	18500	0.0	29.658846	2
CTGTGCG	5630	0.0	28.802883	9
AACGCAG	18920	0.0	28.788551	6
TAGTACT	5880	0.0	28.22474	4
CTAGGCA	6105	0.0	28.108635	4
TCAACGC	20140	0.0	26.612026	4
ACGCAGA	19760	0.0	26.4945	7
ACTGTGC	6420	0.0	26.425892	8
CGCAGAG	19950	0.0	26.218615	8
CTAGTAC	6725	0.0	26.216307	3
GTACTGT	6430	0.0	25.946268	6
>>END_MODULE