FastQCFastQC Report
Mon 16 Jan 2017
ERR1146441_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146441_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4526512
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC170620.3769348231044124No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN140370.31010632469327376No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA132230.2921233833026401No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT130360.28799216703722424No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC117840.2603329009179695No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA115690.2555831068160208No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC106070.2343305397179992No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC95030.2099408993061324No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG77520.1712576924572386No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT74430.16443124418978675No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC73380.16211157730278855No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT68800.15199341126235832No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC66370.14662503932387677No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT61710.13633013675872283No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT60870.13447440324912427No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC58910.13014435839339428No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA58490.12921649163859503No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT57680.12742703432576782No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC55740.12314117360121878No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC54840.12115288769807746No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA54530.12046803366477324No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG54370.1201145606153259No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG51950.1147682807424348No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG51410.11357530920054999No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT50960.11258116624897935No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA49790.10999639457489564No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA47920.10586517830947978No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT47800.10560007352239428No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT47060.10396526066870032No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT46770.10332459076657699No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC46040.1017118699784735No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC45520.10056308256776961No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA45300.10007705712477952No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC45250.036.32531794
GGTATCA64100.032.3218571
GTACTAG43550.031.8602371
CTAACGC24600.030.1847533
TAACGCC25150.029.7078824
GTATCAA95350.029.4995481
TCTAACG25200.029.2795752
CGCCTAC25850.028.6941997
CAACGCA87400.028.225785
AGGGCTA55800.027.7893125
TATTCTC24700.027.7749945
GCATCGT45500.027.4458968
TAGGCAT56050.027.2461915
TACCCCG3450.027.2401165
TAGTACT67000.026.791754
CTATTGA56800.026.780189
GCTATTG56500.026.7555948
CTGTGCG66350.026.5342739
ATCAACG93600.026.3602263
ACGCCTA28800.025.9174656
Produced by FastQC (version 0.11.5)