##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146441_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4526512
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.575611199086627	31.0	31.0	34.0	27.0	34.0
2	30.686061585609405	31.0	31.0	34.0	27.0	34.0
3	30.923112984125524	31.0	31.0	34.0	27.0	34.0
4	34.39166492875751	37.0	35.0	37.0	30.0	37.0
5	34.64192959170328	37.0	35.0	37.0	31.0	37.0
6	34.7657967105798	37.0	35.0	37.0	31.0	37.0
7	34.64140578882813	37.0	35.0	37.0	31.0	37.0
8	34.59678600211377	37.0	35.0	37.0	30.0	37.0
9	35.99590479380149	39.0	35.0	39.0	30.0	39.0
10-11	35.91793725499899	39.0	35.0	39.0	30.0	39.0
12-13	35.906012289374246	39.0	35.0	39.0	30.0	39.0
14-15	36.953481842089445	40.0	36.0	41.0	30.0	41.0
16-17	36.802168755986955	40.0	36.0	41.0	29.5	41.0
18-19	36.75524244716462	40.0	36.0	41.0	29.5	41.0
20-21	37.080737221065576	40.0	36.0	41.0	30.0	41.0
22-23	36.80198108388976	40.0	36.0	41.0	29.5	41.0
24-25	36.922587745266114	40.0	36.0	41.0	30.0	41.0
26-27	36.99584150003358	40.0	36.0	41.0	30.0	41.0
28-29	36.84019638078945	40.0	36.0	41.0	30.0	41.0
30-31	36.7916102950793	40.0	36.0	41.0	30.0	41.0
32-33	36.89247758539025	40.0	36.0	41.0	30.0	41.0
34-35	36.94522426981305	40.0	36.0	41.0	30.0	41.0
36-37	36.88817206272733	40.0	36.0	41.0	30.0	41.0
38-39	36.71860529697038	40.0	35.0	41.0	30.0	41.0
40-41	36.67600803886083	39.5	35.0	41.0	30.0	41.0
42-43	36.524772385448216	39.0	35.0	41.0	29.0	41.0
44-45	36.33912104949683	39.0	35.0	41.0	29.0	41.0
46-47	36.207556502666954	39.0	35.0	41.0	28.5	41.0
48-49	36.0766476483438	39.0	35.0	41.0	28.0	41.0
50-51	35.85555158143842	38.5	34.5	40.5	28.0	41.0
52-53	35.66370585121612	38.0	34.0	40.0	28.0	41.0
54-55	35.4663769807746	38.0	34.0	40.0	28.0	41.0
56-57	35.31641228389542	37.5	34.0	40.0	28.0	41.0
58-59	35.042432009458935	37.0	33.5	40.0	27.0	41.0
60-61	34.78997769143217	36.5	33.0	40.0	26.5	41.0
62-63	34.518222088000655	36.0	33.0	39.5	26.0	41.0
64-65	34.228823981909244	35.0	33.0	39.0	26.0	41.0
66-67	33.937590908849906	35.0	33.0	39.0	26.0	41.0
68-69	33.64766038397778	35.0	32.0	38.0	26.0	40.0
70-71	33.583669942772715	35.0	32.0	37.5	26.0	40.0
72-73	33.37398464866546	35.0	32.0	37.0	26.0	40.0
74-75	33.111598511171515	35.0	32.0	36.5	26.0	39.5
76-77	32.880513958650724	35.0	32.0	36.0	25.0	39.0
78-79	32.665693916198606	35.0	32.0	36.0	25.0	39.0
80-81	32.4523285258053	35.0	32.0	35.5	25.0	38.0
82-83	32.22078059220875	35.0	32.0	35.0	25.0	37.0
84-85	32.04510890504653	35.0	31.5	35.0	24.5	37.0
86-87	31.884091326831786	35.0	31.5	35.0	24.0	36.0
88-89	31.886722270922952	35.0	32.0	35.0	25.0	36.0
90-91	31.81477283170795	35.0	32.0	35.0	24.5	36.0
92-93	31.670408252535285	35.0	32.0	35.0	24.0	36.0
94-95	31.65502212299448	35.0	32.0	35.0	24.0	35.0
96-97	31.572860626460283	35.0	32.0	35.0	24.0	35.0
98-99	31.497990505713894	35.0	32.0	35.0	24.0	35.0
100	31.023848163884246	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7148347852219459
1101	2	0.9513469914891353
1101	3	0.9475596449475496
1101	4	0.9394682969384647
1101	5	0.9144523495066466
1101	6	0.6027451280912359
1101	7	0.659361329561925
1101	8	0.7389037077438729
1101	9	0.9508315818915918
1101	10-11	0.9306795873973073
1101	12-13	0.8739910322333131
1101	14-15	1.1089528322626805
1101	16-17	1.2764667247688806
1101	18-19	1.3213993637133186
1101	20-21	1.0214789092172936
1101	22-23	1.3245785551571672
1101	24-25	1.120046393319484
1101	26-27	1.066347969480823
1101	28-29	1.2273261114575789
1101	30-31	1.2725144019535648
1101	32-33	1.0908554316733614
1101	34-35	1.0229012901856223
1101	36-37	1.002176104435783
1101	38-39	1.0520700413640753
1101	40-41	0.9839792963820528
1101	42-43	1.038780863767272
1101	44-45	1.0322652215925885
1101	46-47	1.0278384892012156
1101	48-49	1.0530533045380963
1101	50-51	1.036045919528334
1101	52-53	1.0456711217231742
1101	54-55	1.0509057707566
1101	56-57	0.9427834589167432
1101	58-59	0.8259085618146926
1101	60-61	0.8873138911715799
1101	62-63	0.7721240249365806
1101	64-65	0.8087896688491725
1101	66-67	0.8457834014858676
1101	68-69	0.8449264516489947
1101	70-71	0.7946483046951229
1101	72-73	0.7090949038354566
1101	74-75	0.7403106953827248
1101	76-77	0.744007822020265
1101	78-79	0.7557624067279889
1101	80-81	0.7606171032421827
1101	82-83	0.697918353927669
1101	84-85	0.6971932425881207
1101	86-87	0.7001101988423457
1101	88-89	0.6643082127154187
1101	90-91	0.6711181917552302
1101	92-93	0.7393040892093978
1101	94-95	0.7525486315504715
1101	96-97	0.6606225372325873
1101	98-99	0.664125781266641
1101	100	0.7171799640176637
1102	1	0.7102140613992525
1102	2	0.8362269848383477
1102	3	0.8267834083571124
1102	4	0.9070874603672721
1102	5	0.7852010848448217
1102	6	0.6220020376230906
1102	7	0.63121487288916
1102	8	0.6840958192337538
1102	9	0.874783720000039
1102	10-11	0.8217250277666963
1102	12-13	0.7401737574420437
1102	14-15	1.000238766857919
1102	16-17	1.1678533261416533
1102	18-19	1.113191843349881
1102	20-21	0.8617637233531141
1102	22-23	1.1356775770795693
1102	24-25	1.0030139532231104
1102	26-27	0.874709819897717
1102	28-29	1.0901774092022052
1102	30-31	1.1496083318977028
1102	32-33	0.9753970451648826
1102	34-35	0.8640929824498826
1102	36-37	0.8089566381958306
1102	38-39	0.8322528315232489
1102	40-41	0.8069286410649781
1102	42-43	0.8390552494624615
1102	44-45	0.8874266728765576
1102	46-47	0.8079511623986662
1102	48-49	0.7669537766611114
1102	50-51	0.790150676643826
1102	52-53	0.7998035726317738
1102	54-55	0.8422860846007652
1102	56-57	0.7238304152244766
1102	58-59	0.7099869968836927
1102	60-61	0.7644128913582691
1102	62-63	0.7198735389527613
1102	64-65	0.6340806205456815
1102	66-67	0.7424328611327979
1102	68-69	0.6920148359886227
1102	70-71	0.5723786270945155
1102	72-73	0.5343608573108227
1102	74-75	0.5892406902230647
1102	76-77	0.5933010344887109
1102	78-79	0.596961447634925
1102	80-81	0.6329104334172797
1102	82-83	0.6558190283469401
1102	84-85	0.6429879855638276
1102	86-87	0.6723180494347503
1102	88-89	0.6377603478929572
1102	90-91	0.6717182988165042
1102	92-93	0.7095305981232087
1102	94-95	0.6552019750459657
1102	96-97	0.6878174142081726
1102	98-99	0.7469003895604516
1102	100	0.803448900036468
1103	1	0.5435055394367438
1103	2	0.7126220025050394
1103	3	0.6969114014593139
1103	4	0.6539314084615455
1103	5	0.6547568406404523
1103	6	0.4332963569987882
1103	7	0.47637977041966195
1103	8	0.48088378832284917
1103	9	0.6192308566772837
1103	10-11	0.7117181876637915
1103	12-13	0.591846319453758
1103	14-15	0.792353843073812
1103	16-17	0.9891870713557722
1103	18-19	1.0240426073253701
1103	20-21	0.7854942631636419
1103	22-23	1.0293797455595595
1103	24-25	0.8755163442734641
1103	26-27	0.7729251064385707
1103	28-29	0.9566078948167132
1103	30-31	0.9503265216766223
1103	32-33	0.7442749890667812
1103	34-35	0.7578632113414443
1103	36-37	0.7759259553764437
1103	38-39	0.7832000507897163
1103	40-41	0.7226745613090202
1103	42-43	0.743654926405739
1103	44-45	0.7913524739499209
1103	46-47	0.7514450412088252
1103	48-49	0.7072214405771575
1103	50-51	0.7559915828981616
1103	52-53	0.7806868601065133
1103	54-55	0.7491698748436662
1103	56-57	0.6157196368438136
1103	58-59	0.5546494836837113
1103	60-61	0.5521739725268091
1103	62-63	0.5968742879747353
1103	64-65	0.5846644935123635
1103	66-67	0.6011752115378215
1103	68-69	0.6274932504303976
1103	70-71	0.5484515131721963
1103	72-73	0.5277691107826783
1103	74-75	0.5785942294317437
1103	76-77	0.5509440778357728
1103	78-79	0.557642861663247
1103	80-81	0.6088372534834647
1103	82-83	0.492542878935474
1103	84-85	0.4477646952699672
1103	86-87	0.5457983489627978
1103	88-89	0.5770712552540971
1103	90-91	0.6319412039182914
1103	92-93	0.628043299201039
1103	94-95	0.6285049053988594
1103	96-97	0.6289175934150002
1103	98-99	0.6596884888410983
1103	100	0.7519964063298978
1104	1	0.507303635583348
1104	2	0.6574877693239323
1104	3	0.6641483459719844
1104	4	0.7405060010789768
1104	5	0.6497797444975859
1104	6	0.3976937175177966
1104	7	0.4377251800851383
1104	8	0.440015249056934
1104	9	0.6203484048978538
1104	10-11	0.6091611522355151
1104	12-13	0.5512680064033546
1104	14-15	0.6954345936082689
1104	16-17	0.8789769942021906
1104	18-19	0.8388845454020668
1104	20-21	0.6971374195747018
1104	22-23	0.9377084756999992
1104	24-25	0.7827383547374893
1104	26-27	0.7118943487869913
1104	28-29	0.9195191968952159
1104	30-31	0.9241109984872296
1104	32-33	0.7377304717752509
1104	34-35	0.6293199630703583
1104	36-37	0.6258864545459986
1104	38-39	0.76500707747023
1104	40-41	0.6445587602634362
1104	42-43	0.6059587131858493
1104	44-45	0.7217061019283548
1104	46-47	0.6423213855195087
1104	48-49	0.6557167443012659
1104	50-51	0.7112726256589426
1104	52-53	0.7465456919010904
1104	54-55	0.7028869998082925
1104	56-57	0.6253856836931035
1104	58-59	0.6544474909937819
1104	60-61	0.6225189767376307
1104	62-63	0.582323421164638
1104	64-65	0.5648247958826857
1104	66-67	0.6859953464470365
1104	68-69	0.603744957943178
1104	70-71	0.5974963676348679
1104	72-73	0.5221685877512598
1104	74-75	0.5367488068569344
1104	76-77	0.49564754388713794
1104	78-79	0.5703176301192485
1104	80-81	0.554573401694725
1104	82-83	0.5432104013543864
1104	84-85	0.490791427081156
1104	86-87	0.4635737736200838
1104	88-89	0.4011128696464965
1104	90-91	0.4370345009560417
1104	92-93	0.5083301341713096
1104	94-95	0.4495811972908932
1104	96-97	0.48804681200347844
1104	98-99	0.5330735494928405
1104	100	0.5820807517711586
1105	1	0.5138044185769921
1105	2	0.6683119473479451
1105	3	0.6227825402905118
1105	4	0.7469484846064844
1105	5	0.5962803985795802
1105	6	0.3669776459581158
1105	7	0.39895184134412887
1105	8	0.4686737543767947
1105	9	0.4826388062316269
1105	10-11	0.6115050717354791
1105	12-13	0.5043150067222868
1105	14-15	0.7222288381825024
1105	16-17	0.796407183483737
1105	18-19	0.8673832126746106
1105	20-21	0.6106397804551733
1105	22-23	0.8819134695141031
1105	24-25	0.6856584741807481
1105	26-27	0.5882455194844809
1105	28-29	0.8220514658149867
1105	30-31	0.7923583677909676
1105	32-33	0.6693487616606149
1105	34-35	0.618602808570202
1105	36-37	0.5294067362113353
1105	38-39	0.6977272520562678
1105	40-41	0.590461968771038
1105	42-43	0.6014023062212033
1105	44-45	0.5978971350242333
1105	46-47	0.49388608989059435
1105	48-49	0.59194477214767
1105	50-51	0.6819025239141823
1105	52-53	0.6301695660572051
1105	54-55	0.6145823871223044
1105	56-57	0.6200284647668681
1105	58-59	0.570124419895258
1105	60-61	0.5990748959039109
1105	62-63	0.5919874046923681
1105	64-65	0.5574379622324699
1105	66-67	0.6457092274590224
1105	68-69	0.6326409891708664
1105	70-71	0.5901742583742262
1105	72-73	0.5343184175445046
1105	74-75	0.5099830406009431
1105	76-77	0.46716553803182137
1105	78-79	0.49516580669912
1105	80-81	0.6222194890630277
1105	82-83	0.5767931250120668
1105	84-85	0.520180844914087
1105	86-87	0.5440667020105252
1105	88-89	0.5096274922144701
1105	90-91	0.5229296743841481
1105	92-93	0.5700474013452066
1105	94-95	0.4790946306076087
1105	96-97	0.4937839277698153
1105	98-99	0.541033368558729
1105	100	0.5738771368158702
1106	1	0.5473550503135556
1106	2	0.5973998288652709
1106	3	0.6175969896120392
1106	4	0.7168680605286326
1106	5	0.5936102519155
1106	6	0.3706058192324164
1106	7	0.42752798638081657
1106	8	0.42422532822657644
1106	9	0.5809873484388319
1106	10-11	0.582496575896684
1106	12-13	0.4706356577855715
1106	14-15	0.678268540287803
1106	16-17	0.8388066997165566
1106	18-19	0.7509891938144193
1106	20-21	0.5615273097224716
1106	22-23	0.9099676250099193
1106	24-25	0.707819446622068
1106	26-27	0.6132569736030931
1106	28-29	0.8141646265645406
1106	30-31	0.7818409941415538
1106	32-33	0.6576667290004821
1106	34-35	0.7094613291301499
1106	36-37	0.5965706762992227
1106	38-39	0.6962860525005965
1106	40-41	0.6149395788773759
1106	42-43	0.6179586618444688
1106	44-45	0.6165847070562691
1106	46-47	0.667065121717684
1106	48-49	0.6350280607371701
1106	50-51	0.6359647148682654
1106	52-53	0.6541660981459074
1106	54-55	0.6109136447648851
1106	56-57	0.597269521363863
1106	58-59	0.5784294188261399
1106	60-61	0.535572799012094
1106	62-63	0.44257452332360714
1106	64-65	0.5578581060512704
1106	66-67	0.6116300696038977
1106	68-69	0.5678386571938958
1106	70-71	0.555160964143532
1106	72-73	0.49080434934875683
1106	74-75	0.5148327280185754
1106	76-77	0.4910185373490563
1106	78-79	0.5038896703342246
1106	80-81	0.6236184274784904
1106	82-83	0.5695556831727515
1106	84-85	0.5643257986106462
1106	86-87	0.5298702322257824
1106	88-89	0.5706840529493036
1106	90-91	0.539831995251955
1106	92-93	0.6128803561311145
1106	94-95	0.5614891593007094
1106	96-97	0.6080800677746403
1106	98-99	0.5940114343622298
1106	100	0.5223650974771559
1107	1	0.4714361610001845
1107	2	0.6192345088640892
1107	3	0.5176285127084483
1107	4	0.6286699559107234
1107	5	0.5794349387646989
1107	6	0.2987895848675848
1107	7	0.320530246679283
1107	8	0.3911099724823188
1107	9	0.5364963599586332
1107	10-11	0.45415003537011955
1107	12-13	0.37920761380115664
1107	14-15	0.5298588760281575
1107	16-17	0.7134759681570415
1107	18-19	0.7429117955166618
1107	20-21	0.5322549385384576
1107	22-23	0.7525999735521722
1107	24-25	0.5147704832309188
1107	26-27	0.5027796447336925
1107	28-29	0.6142229308724794
1107	30-31	0.6831836036753671
1107	32-33	0.561391434847053
1107	34-35	0.3870727731176089
1107	36-37	0.4172217042891688
1107	38-39	0.4678486407585609
1107	40-41	0.4023442975598357
1107	42-43	0.47006055451264217
1107	44-45	0.37055889867672676
1107	46-47	0.41039065032602196
1107	48-49	0.396759051519723
1107	50-51	0.45598417747253706
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2216	90-91	-1.4574141729977903
2216	92-93	-1.4435214981850457
2216	94-95	-1.3625642124820878
2216	96-97	-1.333052709745381
2216	98-99	-1.3155744004550947
2216	100	-1.3541511884453605
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18648.0
1	3253.0
2	476.0
3	478.0
4	466.0
5	239.0
6	236.0
7	321.0
8	548.0
9	927.0
10	1533.0
11	2818.0
12	4450.0
13	6609.0
14	8788.0
15	10967.0
16	13403.0
17	16187.0
18	18531.0
19	21411.0
20	24583.0
21	28221.0
22	33288.0
23	38619.0
24	43218.0
25	49105.0
26	56106.0
27	63357.0
28	76319.0
29	91089.0
30	108008.0
31	130269.0
32	162242.0
33	207725.0
34	290432.0
35	397194.0
36	594332.0
37	938975.0
38	780743.0
39	282043.0
40	355.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.506385997421724	21.122136956576558	12.525436319773172	23.846040726228548
2	15.273859316201117	19.75972078681944	41.23021483587437	23.736205061105068
3	18.305816652795663	26.949334691352238	29.040085903279778	25.704762752572314
4	11.99860252176457	16.844251760942434	40.43431031030853	30.722835406984462
5	12.960634327654446	38.71741429913242	35.200045798548956	13.121905574664172
6	29.445082110974695	38.78497514580609	18.441234650675913	13.328708092543298
7	26.80753637077619	31.91220164905573	23.918169389594468	17.362092590573607
8	25.91322768711161	35.318753962288454	20.104276884439408	18.66374146616053
9	26.115330656582696	15.006386135678342	21.74950579904649	37.12877740869248
10-11	25.595187921026092	25.87709639161822	27.697973677362953	20.829742009992735
12-13	25.836784152784258	23.733086431352376	27.653477201047533	22.77665221481583
14-15	23.25663032801404	24.937462831682982	25.041520237610698	26.764386602692277
16-17	22.107746663214964	27.477721356279382	26.43337019370073	23.981161786804922
18-19	21.53727648259015	28.262633428560385	28.00618228066053	22.193907808188936
20-21	23.41381076884105	27.250769693953714	26.82239751379108	22.513022023414155
22-23	23.915084437427403	26.94895032845011	26.52201458872882	22.613950645393665
24-25	23.292043036258857	26.977308382582184	27.28325703495888	22.44739154620008
26-27	23.160731146425587	27.264847164869018	26.297841752818606	23.276579935886787
28-29	22.24195844526333	27.7704277403478	26.926112932921058	23.06150088146781
30-31	23.04257661389811	27.409257338262634	26.813615135765605	22.734550912073654
32-33	23.558804818684166	27.637909999512523	25.805569000999274	22.997716180804037
34-35	23.51069042306365	27.377775665255673	26.81315847573238	22.298375435948305
36-37	23.18138962865808	27.018603529032276	26.49044609193043	23.309560750379216
38-39	23.30535068823757	27.03081311824625	27.116654699490606	22.547181494025576
40-41	22.88887272186053	26.13848244547654	27.778803952134982	23.193840880527944
42-43	22.894420299593605	26.498384522515057	27.02728818585599	23.579906992035347
44-45	22.980156380293078	27.45273806584163	26.844498680983513	22.72260687288178
46-47	22.937318785725655	27.0932883281207	26.474159342933966	23.495233543219676
48-49	23.655500364839124	26.635143885199657	26.736161736942943	22.97319401301828
50-51	23.26854764978547	26.570522873087615	26.6062627666276	23.554666710499312
52-53	22.673333629478268	27.209437398662907	26.464462608000357	23.652766363858472
54-55	22.646590523899494	27.119519029101973	26.44317036340705	23.790720083591484
56-57	23.862105374910215	26.476533380359708	26.231033053647824	23.430328191082253
58-59	22.683636242482798	26.849422778990157	27.20248546535841	23.264455513168635
60-61	22.78372492083185	27.581032692925305	26.418219268565814	23.21702311767703
62-63	23.21156493488539	26.77585931775155	25.969379137851178	24.043196609511885
64-65	22.292506395208207	27.0258156069956	26.487645259572044	24.194032738224145
66-67	23.160780644993633	26.576796585372247	26.408981499805545	23.85344126982858
68-69	22.528844641481367	26.712806096651764	26.63163055754755	24.12671870431932
70-71	22.53878489363238	26.38814324505922	26.843077914072953	24.229993947235442
72-73	23.189694934951955	26.540304888457566	27.17145480455488	23.0985453720356
74-75	23.347004224545277	26.877594805712903	26.71530109908368	23.060099870658146
76-77	23.024126571387516	26.845416311226174	26.30228917372495	23.828167943661356
78-79	22.88930206477106	26.81324240304258	26.43523016313289	23.862225369053473
80-81	22.449748344712106	27.16877639848534	27.136915535285823	23.244559721516733
82-83	23.328002515971075	26.11677033090861	28.0713897908388	22.483837362281513
84-85	23.60267562332032	26.72511355561632	26.613040620580414	23.059170200482942
86-87	22.217558100621147	27.419364541654247	27.16351370561045	23.19956365211416
88-89	23.330167668531438	26.101956300421364	26.925040319220546	23.642835711826653
90-91	23.001958309770806	26.21314849813959	27.140299218295638	23.644593973793967
92-93	23.361831348963904	26.418659022121627	26.96400971368921	23.255499915225254
94-95	22.13152978562393	26.461172436325253	27.691959710590908	23.715338067459907
96-97	22.561961695847746	26.422891038354525	27.12678202387897	23.88836524191876
98-99	21.979291638586957	27.16261406764907	26.9967269316587	23.86136736210527
100	22.793301879354043	26.386631991792747	26.24984448350319	24.570221645350017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	15813.0
1	9250.5
2	2559.0
3	1892.0
4	996.5
5	538.5
6	421.5
7	407.0
8	415.0
9	415.0
10	371.5
11	347.5
12	352.0
13	348.0
14	369.0
15	474.0
16	564.0
17	634.5
18	808.5
19	1014.5
20	1280.0
21	1635.5
22	2232.0
23	3137.0
24	4166.5
25	5592.5
26	8000.0
27	10837.5
28	15394.5
29	24695.0
30	37198.5
31	48844.5
32	64088.0
33	78138.0
34	88674.0
35	101703.5
36	114182.5
37	135911.0
38	145659.0
39	136239.0
40	126591.5
41	117104.5
42	116693.0
43	125258.5
44	137719.5
45	163211.0
46	189118.0
47	206421.0
48	215599.0
49	209957.5
50	200080.0
51	203774.5
52	206516.5
53	199146.0
54	188659.5
55	163944.0
56	143704.0
57	118776.5
58	93573.5
59	88934.0
60	74182.0
61	51531.0
62	38231.0
63	31335.0
64	22966.5
65	11886.0
66	6790.0
67	4642.5
68	3341.0
69	2440.0
70	1740.0
71	1303.0
72	1003.0
73	766.5
74	561.0
75	393.5
76	278.0
77	164.5
78	110.5
79	92.0
80	64.5
81	43.0
82	31.0
83	24.5
84	18.5
85	13.0
86	14.0
87	10.0
88	8.0
89	8.5
90	6.0
91	3.5
92	3.5
93	3.5
94	3.0
95	4.0
96	3.5
97	2.5
98	2.0
99	2.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9990694821973298
2	0.45114207142276436
3	0.4840592491525484
4	0.46954476205961676
5	0.437842647937308
6	0.4913717228629903
7	0.5073442862848921
8	0.510702280254642
9	0.5255481483314305
10-11	0.537754014570159
12-13	0.5372900811927594
14-15	0.5343960206004094
16-17	0.5387481575217298
18-19	0.5366162731922505
20-21	0.5354122556175704
22-23	0.4982975854255992
24-25	0.5329158522058486
26-27	0.513154499535183
28-29	0.5194176001300781
30-31	0.5222785226240425
32-33	0.5246313276094264
34-35	0.5359093270933557
36-37	0.554687582845246
38-39	0.5437851484763544
40-41	0.549970926841683
42-43	0.5467123471670903
44-45	0.5576368736015722
46-47	0.5546765368124507
48-49	0.5267300738405201
50-51	0.5421392895898652
52-53	0.5348930920761946
54-55	0.543862470705921
56-57	0.5318443870247113
58-59	0.537654600275002
60-61	0.5317560187623495
62-63	0.5257359308889493
64-65	0.537124390700831
66-67	0.5354012095847752
68-69	0.555748001993588
70-71	0.5398638068340479
72-73	0.5428130975903742
74-75	0.5410125942447519
76-77	0.5276248024969337
78-79	0.5597024817342802
80-81	0.5329268982386438
82-83	0.5318775251230969
84-85	0.5419846451307321
86-87	0.8472307153941048
88-89	1.1125343310699276
90-91	1.1034434460794538
92-93	1.1033440317842966
94-95	1.1079060433287264
96-97	1.1022946586687499
98-99	1.0973791740748726
100	1.093446786399771
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4526512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.391689414922606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73556993457186	20.650857109997467
2	12.969044040554737	7.3642614081576605
3	5.350815920709802	4.557561112116475
4	2.7477155497986323	3.120491459617443
5	1.5656813144339619	2.222616880107841
6	0.9836609332738835	1.6756677424263007
7	0.6523360877398701	1.2964646518079266
8	0.4591787560821323	1.0429488502891524
9	0.33767041655507113	0.8628330232295207
>10	1.7024718476009277	8.754665898327014
>50	0.18719299815052248	3.7613010742321666
>100	0.2277260321485319	14.441210491454598
>500	0.04641584296166802	9.345823784713271
>1k	0.032576613401453576	16.399803393645456
>5k	0.0013994726520910844	2.4877298025022774
>10k+	5.442393647020884E-4	2.015763317375513
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	17062	0.3769348231044124	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	14037	0.31010632469327376	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13223	0.2921233833026401	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	13036	0.28799216703722424	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11784	0.2603329009179695	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11569	0.2555831068160208	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10607	0.2343305397179992	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9503	0.2099408993061324	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7752	0.1712576924572386	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7443	0.16443124418978675	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7338	0.16211157730278855	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6880	0.15199341126235832	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6637	0.14662503932387677	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6171	0.13633013675872283	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6087	0.13447440324912427	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5891	0.13014435839339428	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5849	0.12921649163859503	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5768	0.12742703432576782	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5574	0.12314117360121878	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5484	0.12115288769807746	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5453	0.12046803366477324	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5437	0.1201145606153259	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5195	0.1147682807424348	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5141	0.11357530920054999	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5096	0.11258116624897935	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4979	0.10999639457489564	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4792	0.10586517830947978	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4780	0.10560007352239428	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4706	0.10396526066870032	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4677	0.10332459076657699	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4604	0.1017118699784735	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4552	0.10056308256776961	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	4530	0.10007705712477952	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.2092065590459057E-5	0.0
5	0.0	0.0	0.0	2.2092065590459057E-5	0.0
6	0.0	0.0	0.0	2.2092065590459057E-5	0.0
7	0.0	0.0	0.0	2.2092065590459057E-5	0.0
8	0.0	0.0	0.0	2.2092065590459057E-5	0.0
9	0.0	0.0	0.0	1.1046032795229528E-4	0.0
10-11	0.0	0.0	0.0	1.546444591332134E-4	0.0
12-13	0.0	0.0	0.0	1.546444591332134E-4	0.0
14-15	0.0	0.0	0.0	1.7673652472367245E-4	0.0
16-17	0.0	0.0	0.0	1.988285903141315E-4	0.0
18-19	0.0	0.0	0.0	2.761508198807382E-4	0.0
20-21	0.0	0.0	0.0	3.7556511503780395E-4	0.0
22-23	0.0	0.0	0.0	4.860254429900992E-4	0.0
24-25	0.0	0.0	0.0	7.511302300756079E-4	0.0
26-27	0.0	0.0	0.0	0.0011377413779086413	0.0
28-29	0.0	0.0	0.0	0.002385943083769578	0.0
30-31	0.0	0.0	0.0	0.006892724464223225	0.0
32-33	0.0	0.0	0.0	0.018590473194371293	0.0
34-35	0.0	0.0	0.0	0.03725826861830919	0.0
36-37	0.0	0.0	0.0	0.07063937972549283	0.0
38-39	0.0	0.0	0.0	0.12218016874803381	0.0
40-41	0.0	0.0	0.0	0.18009451869342225	0.0
42-43	0.0	0.0	0.0	0.2434545628068588	0.0
44-45	0.0	0.0	0.0	0.31904256522461444	0.0
46-47	0.0	0.0	0.0	0.41700983008550513	0.0
48-49	0.0	0.0	0.0	0.5296130884000749	0.0
50-51	0.0	0.0	0.0	0.6535827144609359	0.0
52-53	0.0	0.0	0.0	0.775674514946608	0.0
54-55	0.0	0.0	0.0	0.9089007164898713	0.0
56-57	2.2092065590459057E-5	0.0	0.0	1.0753975688123658	0.0
58-59	2.2092065590459057E-5	0.0	0.0	1.2790643214908077	0.0
60-61	2.2092065590459057E-5	0.0	0.0	1.4940201196859746	0.0
62-63	2.2092065590459057E-5	0.0	0.0	1.7211707380870744	0.0
64-65	2.2092065590459057E-5	0.0	0.0	1.9569924922324295	0.0
66-67	2.2092065590459057E-5	0.0	0.0	2.2267807972231157	0.0
68-69	2.2092065590459057E-5	0.0	0.0	2.534070383553606	0.0
70-71	2.2092065590459057E-5	0.0	0.0	2.8802751434216898	0.0
72-73	2.2092065590459057E-5	0.0	0.0	3.239613636283302	0.0
74-75	2.2092065590459057E-5	0.0	0.0	3.5889002393012546	0.0
76-77	2.2092065590459057E-5	0.0	0.0	3.9972720717408903	0.0
78-79	2.2092065590459057E-5	0.0	0.0	4.435865849908273	0.0
80-81	2.2092065590459057E-5	0.0	0.0	4.917914721092091	0.0
82-83	2.2092065590459057E-5	0.0	0.0	5.423546872293722	0.0
84-85	2.2092065590459057E-5	0.0	0.0	5.910455997907439	0.0
86-87	2.2092065590459057E-5	0.0	0.0	6.429619539283227	0.0
88	2.2092065590459057E-5	0.0	0.0	6.850926276126077	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4525	0.0	36.325317	94
GGTATCA	6410	0.0	32.321857	1
GTACTAG	4355	0.0	31.860237	1
CTAACGC	2460	0.0	30.184753	3
TAACGCC	2515	0.0	29.707882	4
GTATCAA	9535	0.0	29.499548	1
TCTAACG	2520	0.0	29.279575	2
CGCCTAC	2585	0.0	28.694199	7
CAACGCA	8740	0.0	28.22578	5
AGGGCTA	5580	0.0	27.789312	5
TATTCTC	2470	0.0	27.774994	5
GCATCGT	4550	0.0	27.445896	8
TAGGCAT	5605	0.0	27.246191	5
TACCCCG	345	0.0	27.240116	5
TAGTACT	6700	0.0	26.79175	4
CTATTGA	5680	0.0	26.78018	9
GCTATTG	5650	0.0	26.755594	8
CTGTGCG	6635	0.0	26.534273	9
ATCAACG	9360	0.0	26.360226	3
ACGCCTA	2880	0.0	25.917465	6
>>END_MODULE