##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146441_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4526512 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.892331667297025 31.0 31.0 34.0 30.0 34.0 2 31.895669778407747 33.0 31.0 34.0 30.0 34.0 3 32.073762093196706 33.0 31.0 34.0 30.0 34.0 4 35.78603282174001 37.0 35.0 37.0 35.0 37.0 5 35.69344033551662 37.0 35.0 37.0 33.0 37.0 6 35.72310268922296 37.0 35.0 37.0 33.0 37.0 7 35.64570269558548 37.0 35.0 37.0 33.0 37.0 8 35.63171775530475 37.0 35.0 37.0 33.0 37.0 9 37.245073690293985 39.0 37.0 39.0 33.0 39.0 10-11 37.19011912483607 39.0 37.0 39.0 33.0 39.0 12-13 37.118439429741926 39.0 37.0 39.0 33.0 39.0 14-15 38.325759547307065 40.0 38.0 41.0 33.0 41.0 16-17 38.33074009303411 40.0 38.0 41.0 33.0 41.0 18-19 38.39136723817367 40.0 38.0 41.0 33.5 41.0 20-21 38.40606950782413 40.0 38.0 41.0 34.0 41.0 22-23 38.3728486746528 40.0 38.0 41.0 34.0 41.0 24-25 38.300535158196865 40.0 38.0 41.0 33.0 41.0 26-27 38.15693595863658 40.0 37.0 41.0 33.0 41.0 28-29 38.09537796431336 40.0 37.0 41.0 33.0 41.0 30-31 37.97187348669351 40.0 37.0 41.0 33.0 41.0 32-33 37.847431753190975 40.0 37.0 41.0 33.0 41.0 34-35 37.74731316298289 40.0 36.5 41.0 33.0 41.0 36-37 37.66676538137975 40.0 36.0 41.0 32.0 41.0 38-39 37.555858904162854 40.0 36.0 41.0 32.0 41.0 40-41 37.42810093069454 40.0 36.0 41.0 32.0 41.0 42-43 37.3023092615241 40.0 36.0 41.0 31.0 41.0 44-45 37.09617493557954 39.0 35.0 41.0 31.0 41.0 46-47 36.851967806558335 39.0 35.0 41.0 30.5 41.0 48-49 36.580323878518385 39.0 35.0 41.0 30.0 41.0 50-51 36.37826553867525 39.0 35.0 41.0 30.0 41.0 52-53 36.18140921751671 38.5 35.0 41.0 29.0 41.0 54-55 35.97771628574054 38.0 35.0 40.0 29.0 41.0 56-57 35.7147457026514 38.0 34.5 40.0 28.5 41.0 58-59 35.42416224678074 37.0 34.0 40.0 28.0 41.0 60-61 35.16445212119177 37.0 34.0 40.0 28.0 41.0 62-63 34.88650598960082 36.0 34.0 39.5 28.0 41.0 64-65 34.608221297104706 35.5 34.0 39.0 27.0 41.0 66-67 34.38324509025934 35.0 33.0 39.0 27.0 41.0 68-69 34.18807096943519 35.0 33.0 38.5 27.0 41.0 70-71 33.9842558685363 35.0 33.0 38.0 27.0 40.5 72-73 33.7813705122178 35.0 33.0 37.0 26.5 40.0 74-75 33.597182002389474 35.0 33.0 37.0 26.5 39.5 76-77 33.439705450907894 35.0 33.0 36.0 27.0 39.0 78-79 33.32912715132535 35.0 33.0 36.0 27.0 39.0 80-81 33.184025801765245 35.0 33.0 36.0 27.0 38.0 82-83 32.990952967759725 35.0 33.0 35.0 27.0 37.0 84-85 32.84726319073052 35.0 33.0 35.0 27.0 37.0 86-87 32.715410563365346 35.0 33.0 35.0 27.0 36.5 88-89 32.60610056926835 35.0 33.0 35.0 27.0 36.0 90-91 32.517459801277454 35.0 33.0 35.0 26.5 36.0 92-93 32.4366215090118 35.0 33.0 35.0 26.0 36.0 94-95 32.39410345095738 35.0 33.0 35.0 26.0 35.0 96-97 32.34105763996649 35.0 33.0 35.0 26.0 35.0 98-99 32.29789228439027 35.0 33.0 35.0 26.0 35.0 100 31.80050400838438 35.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5149991615523426 1101 2 0.629673736587602 1101 3 0.49446463641537264 1101 4 0.3960951972028184 1101 5 0.380676489303184 1101 6 0.3727903521277014 1101 7 0.3935596193992197 1101 8 0.4189714421271944 1101 9 0.5009741833859707 1101 10-11 0.5250301889982723 1101 12-13 0.5565088340191764 1101 14-15 0.6913472325268444 1101 16-17 0.6456154689932205 1101 18-19 0.6588927583521524 1101 20-21 0.6231362511253806 1101 22-23 0.6777927080183375 1101 24-25 0.663131285140409 1101 26-27 0.7168271935442689 1101 28-29 0.7280680881521491 1101 30-31 0.7590737432448051 1101 32-33 0.7330568119319381 1101 34-35 0.7929143496736302 1101 36-37 0.7790876901770147 1101 38-39 0.7998274866393515 1101 40-41 0.801938072535826 1101 42-43 0.8209386064023647 1101 44-45 0.8171581574013942 1101 46-47 0.8325646931370798 1101 48-49 0.769109280837796 1101 50-51 0.7522066720623144 1101 52-53 0.762274708899362 1101 54-55 0.750630362451929 1101 56-57 0.7994241042047747 1101 58-59 0.7341597292288498 1101 60-61 0.7630993435508557 1101 62-63 0.7316287425346246 1101 64-65 0.6656283386834048 1101 66-67 0.7312335097984999 1101 68-69 0.7556628852689258 1101 70-71 0.7297500132308201 1101 72-73 0.6291060053402475 1101 74-75 0.6541945905829536 1101 76-77 0.6632745580183439 1101 78-79 0.5826955497882125 1101 80-81 0.6047599985841501 1101 82-83 0.6029271212094756 1101 84-85 0.5534060480606726 1101 86-87 0.5981941144718732 1101 88-89 0.5599104711288732 1101 90-91 0.5973999166217681 1101 92-93 0.5625635449617974 1101 94-95 0.5294952654677019 1101 96-97 0.5246462650926773 1101 98-99 0.55754036566438 1101 100 0.6199796548369889 1102 1 0.3990853303457236 1102 2 0.5193412583210808 1102 3 0.4306425412568089 1102 4 0.2884121666884738 1102 5 0.29633137873060633 1102 6 0.3355589193451891 1102 7 0.35239881282760166 1102 8 0.3726660722440158 1102 9 0.3792238826050607 1102 10-11 0.4093417054701618 1102 12-13 0.4396857072267082 1102 14-15 0.5511843994413326 1102 16-17 0.6222037845101767 1102 18-19 0.5959447451962205 1102 20-21 0.5716192174423256 1102 22-23 0.51816406926892 1102 24-25 0.5169137374203387 1102 26-27 0.519330239647573 1102 28-29 0.542181144634263 1102 30-31 0.6175977708247018 1102 32-33 0.6352480359796218 1102 34-35 0.5248858401062932 1102 36-37 0.5955102956201941 1102 38-39 0.6436457891709253 1102 40-41 0.5882757841483865 1102 42-43 0.6062722602377733 1102 44-45 0.5740573669270503 1102 46-47 0.541829027472204 1102 48-49 0.4864098747236483 1102 50-51 0.4926505056910955 1102 52-53 0.48383625332177616 1102 54-55 0.5268657120716753 1102 56-57 0.5378542813812999 1102 58-59 0.5181167052472091 1102 60-61 0.6025525737296462 1102 62-63 0.5315934369596675 1102 64-65 0.530193036112081 1102 66-67 0.49392626298426734 1102 68-69 0.5276900833335247 1102 70-71 0.4807297359831111 1102 72-73 0.438991378067044 1102 74-75 0.44172003325255815 1102 76-77 0.4435084142912089 1102 78-79 0.42817429257173245 1102 80-81 0.37445458557182576 1102 82-83 0.4232658258194135 1102 84-85 0.4242460049777321 1102 86-87 0.4650426245671966 1102 88-89 0.41848532949475725 1102 90-91 0.41258830308789385 1102 92-93 0.4192299169696483 1102 94-95 0.4529844390879134 1102 96-97 0.47536888803996646 1102 98-99 0.48339261460361627 1102 100 0.5033107853603056 1103 1 0.2934514505330519 1103 2 -0.11650601958205087 1103 3 0.2111256158498378 1103 4 0.1705479759938413 1103 5 0.23845921338261888 1103 6 0.2869294714280315 1103 7 0.29003869726324893 1103 8 0.32018474564924304 1103 9 0.3806939202125932 1103 10-11 0.3753158099658762 1103 12-13 0.35010969015983306 1103 14-15 0.5054867425865766 1103 16-17 0.5230486377217218 1103 18-19 0.5178748064266259 1103 20-21 0.4954297813265143 1103 22-23 0.45965084927512834 1103 24-25 0.41044069817414197 1103 26-27 0.5113772218106618 1103 28-29 0.5364533258945556 1103 30-31 0.5897728789728376 1103 32-33 0.5882669452098739 1103 34-35 0.5175275571478934 1103 36-37 0.5645117743204224 1103 38-39 0.5686622649628745 1103 40-41 0.57934479475648 1103 42-43 0.6088889170868086 1103 44-45 0.5581098053610702 1103 46-47 0.6601305751705979 1103 48-49 0.6566808556648738 1103 50-51 0.6195303355203023 1103 52-53 0.6101276770286788 1103 54-55 0.6383428631170958 1103 56-57 0.5690320263289834 1103 58-59 0.5335513431986172 1103 60-61 0.5701347710267086 1103 62-63 0.6375991574824553 1103 64-65 0.5578032128193655 1103 66-67 0.5759745294012646 1103 68-69 0.5492458059925411 1103 70-71 0.4543772155990453 1103 72-73 0.48268431202964734 1103 74-75 0.4888775512430499 1103 76-77 0.5112774087178664 1103 78-79 0.42752924302997997 1103 80-81 0.42712083379766597 1103 82-83 0.4374088298062375 1103 84-85 0.46196675872292303 1103 86-87 0.5065579197202013 1103 88-89 0.43124950565637477 1103 90-91 0.42913999805075775 1103 92-93 0.3827280270053919 1103 94-95 0.4068947147340509 1103 96-97 0.3909175235012441 1103 98-99 0.5071096390503627 1103 100 0.4651350550176332 1104 1 0.273787239015828 1104 2 0.3749274979129673 1104 3 0.3189894659739565 1104 4 0.23105282735147625 1104 5 0.22356762961588572 1104 6 0.23931373000313982 1104 7 0.265893294460092 1104 8 0.24150853559552843 1104 9 0.2910176928332078 1104 10-11 0.31413973749836543 1104 12-13 0.3088889259550811 1104 14-15 0.3992907430499528 1104 16-17 0.3952593533772273 1104 18-19 0.4378406544723319 1104 20-21 0.4182914368656796 1104 22-23 0.36746832350490166 1104 24-25 0.38171679581482465 1104 26-27 0.43058373412259243 1104 28-29 0.4537514665443467 1104 30-31 0.4745233884664586 1104 32-33 0.48712636828542344 1104 34-35 0.5039800504987468 1104 36-37 0.48435296671384265 1104 38-39 0.4604929224992489 1104 40-41 0.4355561665792962 1104 42-43 0.4629792106686068 1104 44-45 0.516816341283473 1104 46-47 0.4677972651080182 1104 48-49 0.39533247770435764 1104 50-51 0.4095471991730548 1104 52-53 0.3968549351325876 1104 54-55 0.3734600695803252 1104 56-57 0.36904416703788456 1104 58-59 0.3522136978116066 1104 60-61 0.40766395289154644 1104 62-63 0.3702627220047816 1104 64-65 0.36993041364664947 1104 66-67 0.3459908596951351 1104 68-69 0.33113890473629226 1104 70-71 0.4157277210500112 1104 72-73 0.41961543127816014 1104 74-75 0.41133056725163186 1104 76-77 0.3499227586947171 1104 78-79 0.3440007623452175 1104 80-81 0.32394924664053804 1104 82-83 0.34070999429513193 1104 84-85 0.3172547954492657 1104 86-87 0.3158895089360314 1104 88-89 0.35571478365355347 1104 90-91 0.3293804426886169 1104 92-93 0.32607013273343455 1104 94-95 0.30150047334532815 1104 96-97 0.29102057302311124 1104 98-99 0.28446980305312053 1104 100 0.34832572334349265 1105 1 0.12350069775953543 1105 2 -0.4437172828614102 1105 3 0.030100241033338193 1105 4 0.10721461774915042 1105 5 0.1851496449205925 1105 6 0.2302744411624218 1105 7 0.22547445225443852 1105 8 0.20272102483688315 1105 9 0.26175613304276624 1105 10-11 0.3245631015279642 1105 12-13 0.29365857261692696 1105 14-15 0.30224641649079587 1105 16-17 0.37108254204567004 1105 18-19 0.35307260061305357 1105 20-21 0.39235633470754294 1105 22-23 0.3453234923434181 1105 24-25 0.4397960585459799 1105 26-27 0.4392915919444462 1105 28-29 0.4335522819353059 1105 30-31 0.3885588010744243 1105 32-33 0.4146698105809108 1105 34-35 0.3986813087053491 1105 36-37 0.467956365816498 1105 38-39 0.3916440212871777 1105 40-41 0.4283756535415151 1105 42-43 0.47627918439459194 1105 44-45 0.4320761976221874 1105 46-47 0.3694687492277353 1105 48-49 0.4073934638214354 1105 50-51 0.4338080509823783 1105 52-53 0.354937742934041 1105 54-55 0.35949117042422785 1105 56-57 0.39002540531110697 1105 58-59 0.37505830849698896 1105 60-61 0.40161589975284784 1105 62-63 0.39172245180349563 1105 64-65 0.3761031846723313 1105 66-67 0.4462915378489072 1105 68-69 0.44193200968199164 1105 70-71 0.38458458834080034 1105 72-73 0.33901990037227137 1105 74-75 0.2822905247735932 1105 76-77 0.25908942215924924 1105 78-79 0.2748311314337144 1105 80-81 0.2973865885352609 1105 82-83 0.3027726599822316 1105 84-85 0.24830677274941593 1105 86-87 0.23829577043917283 1105 88-89 0.22592215745763156 1105 90-91 0.20523778261413383 1105 92-93 0.1341723991695929 1105 94-95 0.18387357397992332 1105 96-97 0.19980969476416988 1105 98-99 0.2881358829652072 1105 100 0.12364398120339004 1106 1 0.21580698466815917 1106 2 0.2684892270663788 1106 3 0.22198346331031615 1106 4 0.19914984056638474 1106 5 0.2077631620596705 1106 6 0.23581353105527114 1106 7 0.2500888269038768 1106 8 0.2290374074847179 1106 9 0.3083867891086953 1106 10-11 0.28039875967724015 1106 12-13 0.2998054735891884 1106 14-15 0.43049405065757895 1106 16-17 0.3370043309367645 1106 18-19 0.3419242810322132 1106 20-21 0.35864643809726715 1106 22-23 0.33201067923101846 1106 24-25 0.25791480731408 1106 26-27 0.34566997236984065 1106 28-29 0.3474498921363107 1106 30-31 0.3446227157109547 1106 32-33 0.3945336160998991 1106 34-35 0.34169295680622014 1106 36-37 0.39259610117876065 1106 38-39 0.40864105706501874 1106 40-41 0.33722815380262716 1106 42-43 0.3297111847998764 1106 44-45 0.4313165848494549 1106 46-47 0.38324704926027664 1106 48-49 0.3680591392246555 1106 50-51 0.3605401660144665 1106 52-53 0.3462949167474889 1106 54-55 0.3510819488339436 1106 56-57 0.42871734244756254 1106 58-59 0.37338896324351367 1106 60-61 0.40765891043405844 1106 62-63 0.2859489515728981 1106 64-65 0.28861229080993667 1106 66-67 0.2994074760900247 1106 68-69 0.31602543721858467 1106 70-71 0.3005604978793954 1106 72-73 0.3187268280266622 1106 74-75 0.2985579918638379 1106 76-77 0.3261865360638012 1106 78-79 0.3323105122576564 1106 80-81 0.3113989061065823 1106 82-83 0.29282333407058303 1106 84-85 0.269039366996239 1106 86-87 0.29171052797695296 1106 88-89 0.2747063288275555 1106 90-91 0.327545782730553 1106 92-93 0.2984960804251173 1106 94-95 0.31622243223921487 1106 96-97 0.2577437152136923 1106 98-99 0.3214022591822143 1106 100 0.2508843579560178 1107 1 0.02447980266298444 1107 2 -0.5573027497240304 1107 3 -0.10488921588745015 1107 4 0.006587024757592985 1107 5 0.08975428847205791 1107 6 0.13294570468460165 1107 7 0.1418133430750501 1107 8 0.16770234917891713 1107 9 0.17865770581644114 1107 10-11 0.18953667009259334 1107 12-13 0.17137703862294273 1107 14-15 0.18717788652633516 1107 16-17 0.30210068812536406 1107 18-19 0.2715911531805588 1107 20-21 0.1998954684712757 1107 22-23 0.25037212239833906 1107 24-25 0.23399119281679504 1107 26-27 0.255116373711644 1107 28-29 0.18123340630247498 1107 30-31 0.27267382786189387 1107 32-33 0.2827397729751695 1107 34-35 0.2835840801468237 1107 36-37 0.27684156142044714 1107 38-39 0.3393535297432635 1107 40-41 0.28505844323356655 1107 42-43 0.27819880684003806 1107 44-45 0.3401475849666582 1107 46-47 0.27773226695603626 1107 48-49 0.3125885101316186 1107 50-51 0.25164039365476043 1107 52-53 0.2619688742858486 1107 54-55 0.22828755905040765 1107 56-57 0.23445925805572898 1107 58-59 0.2018341290111607 1107 60-61 0.21382929560335384 1107 62-63 0.20632945353870014 1107 64-65 0.2639204611372463 1107 66-67 0.18453632861054814 1107 68-69 0.2130659940530535 1107 70-71 0.2590554057213197 1107 72-73 0.18826305080816752 1107 74-75 0.20488175762426408 1107 76-77 0.16039234639377753 1107 78-79 0.12262361682934397 1107 80-81 0.19890460606097804 1107 82-83 0.18642205787725885 1107 84-85 0.1986106260442142 1107 86-87 0.2158291562182555 1107 88-89 0.17890101809541648 1107 90-91 0.13028596591046693 1107 92-93 0.14297108368329958 1107 94-95 0.16519184767814465 1107 96-97 0.1765829256510898 1107 98-99 0.2276344081477859 1107 100 0.2592649684781705 1108 1 0.13575533554601904 1108 2 0.2989600633724798 1108 3 0.15224846606304254 1108 4 0.11851649310682433 1108 5 0.09258214277429744 1108 6 0.10428853290797235 1108 7 0.06935945689959055 1108 8 0.11187726679469989 1108 9 0.07319626492255793 1108 10-11 0.11243707014934046 1108 12-13 0.09046356081135798 1108 14-15 0.09870267091117313 1108 16-17 0.10345175308012244 1108 18-19 0.16779610831958536 1108 20-21 0.14350252249537476 1108 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2214 4 -0.21881400213422353 2214 5 -0.24742122695061397 2214 6 -0.36139894720266597 2214 7 -0.38690102046890473 2214 8 -0.37408338989128964 2214 9 -0.5253320452057721 2214 10-11 -0.4240083396165062 2214 12-13 -0.46932308312216264 2214 14-15 -0.6327106524839721 2214 16-17 -0.5613722448854048 2214 18-19 -0.635715626431768 2214 20-21 -0.5804652674326505 2214 22-23 -0.5705135458834079 2214 24-25 -0.5749584103541707 2214 26-27 -0.562943905764115 2214 28-29 -0.6361649106673823 2214 30-31 -0.5636748513966552 2214 32-33 -0.5911130436724292 2214 34-35 -0.5576377795613112 2214 36-37 -0.5596736301639922 2214 38-39 -0.5840119856409132 2214 40-41 -0.5354671130225412 2214 42-43 -0.6590090772046793 2214 44-45 -0.5596829017739111 2214 46-47 -0.603533576171543 2214 48-49 -0.5246032241523011 2214 50-51 -0.5193869552149195 2214 52-53 -0.525191769734235 2214 54-55 -0.4765254349128796 2214 56-57 -0.5798952534776731 2214 58-59 -0.5404526277605086 2214 60-61 -0.5376430028679167 2214 62-63 -0.4600241979903359 2214 64-65 -0.5314712401561437 2214 66-67 -0.4825127892680001 2214 68-69 -0.47116899641750365 2214 70-71 -0.4436317130971972 2214 72-73 -0.4731349439561896 2214 74-75 -0.36851592401637134 2214 76-77 -0.4053098360252818 2214 78-79 -0.4043696817109321 2214 80-81 -0.4455265743888077 2214 82-83 -0.3919694733303274 2214 84-85 -0.4263544461494604 2214 86-87 -0.39697264860635784 2214 88-89 -0.4027559434139505 2214 90-91 -0.38305039007369146 2214 92-93 -0.42037877156607806 2214 94-95 -0.42516336907464947 2214 96-97 -0.5041074193684167 2214 98-99 -0.4906337846910205 2214 100 -0.6651993229298512 2215 1 -0.549121076822086 2215 2 -0.35915599592767933 2215 3 -0.42323121415916276 2215 4 -0.32755013623470575 2215 5 -0.4990459371660094 2215 6 -0.45326350246059377 2215 7 -0.45192072404399397 2215 8 -0.5976811722166744 2215 9 -0.763445136969942 2215 10-11 -0.6844124318275817 2215 12-13 -0.6364292556182392 2215 14-15 -0.7782169215518735 2215 16-17 -0.8010591727991283 2215 18-19 -0.826260054012586 2215 20-21 -0.7734174114337193 2215 22-23 -0.7321737039624665 2215 24-25 -0.8047978676079026 2215 26-27 -0.8570233993073515 2215 28-29 -0.8064272805730113 2215 30-31 -0.8208088278045196 2215 32-33 -0.8962995312044342 2215 34-35 -0.8823276552073125 2215 36-37 -0.8150884286493465 2215 38-39 -0.851498508458775 2215 40-41 -0.9488306138537155 2215 42-43 -0.9303792471813495 2215 44-45 -0.9781452879592365 2215 46-47 -0.9857167473071655 2215 48-49 -0.8583558849153405 2215 50-51 -0.8629539839205407 2215 52-53 -0.9395760710446339 2215 54-55 -0.9405349277172164 2215 56-57 -0.9479542922877755 2215 58-59 -0.9186229513669844 2215 60-61 -0.9164785715649018 2215 62-63 -0.8273380058242665 2215 64-65 -0.8478206140001134 2215 66-67 -0.8140650725948575 2215 68-69 -0.7658886042505628 2215 70-71 -0.7414008606138083 2215 72-73 -0.7609347305024414 2215 74-75 -0.7220583988233287 2215 76-77 -0.7316072037319614 2215 78-79 -0.6993259082816934 2215 80-81 -0.6465978241601249 2215 82-83 -0.6650174601200547 2215 84-85 -0.6764942954989976 2215 86-87 -0.7087636271230835 2215 88-89 -0.6704824140103263 2215 90-91 -0.6517178925897369 2215 92-93 -0.7003436457401904 2215 94-95 -0.755352336099957 2215 96-97 -0.686610632831254 2215 98-99 -0.7405077553061616 2215 100 -0.8150738664956343 2216 1 -0.295034615948218 2216 2 -0.14247766768561831 2216 3 -0.3018619646575935 2216 4 -0.24399366242798948 2216 5 -0.37587840595985966 2216 6 -0.3647458012279259 2216 7 -0.37731095399344383 2216 8 -0.4848877438544861 2216 9 -0.5073276699086406 2216 10-11 -0.5443822997018088 2216 12-13 -0.5287966865076399 2216 14-15 -0.6057812871411485 2216 16-17 -0.7138730058266418 2216 18-19 -0.659638469289817 2216 20-21 -0.621782904992898 2216 22-23 -0.6480834812102998 2216 24-25 -0.7025720710654184 2216 26-27 -0.7116395467432994 2216 28-29 -0.7315916308773325 2216 30-31 -0.751139045778288 2216 32-33 -0.7466211087299186 2216 34-35 -0.7424853016515982 2216 36-37 -0.7274055379241204 2216 38-39 -0.8756048954158118 2216 40-41 -0.7921738329382322 2216 42-43 -0.8735916538114594 2216 44-45 -0.7714982270843294 2216 46-47 -0.6447082671282516 2216 48-49 -0.6885962667125938 2216 50-51 -0.758259649517008 2216 52-53 -0.8013076406234276 2216 54-55 -0.8723837536327679 2216 56-57 -0.7896967282783436 2216 58-59 -0.8165717631105736 2216 60-61 -0.8475219511413812 2216 62-63 -0.8869124127348442 2216 64-65 -0.7679077270812869 2216 66-67 -0.7682205197872065 2216 68-69 -0.8327713501397653 2216 70-71 -0.7903014572462226 2216 72-73 -0.7781364198603313 2216 74-75 -0.9065673995067058 2216 76-77 -0.8461036425613742 2216 78-79 -0.7111026092702417 2216 80-81 -0.6997568558956857 2216 82-83 -0.6650281076454192 2216 84-85 -0.6284264132584099 2216 86-87 -0.6612599524283524 2216 88-89 -0.5942187345372005 2216 90-91 -0.6543739864244884 2216 92-93 -0.6097583768697703 2216 94-95 -0.5676640288635717 2216 96-97 -0.5640554270817404 2216 98-99 -0.6433053091206844 2216 100 -0.6427036012127267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 4.0 12 6.0 13 52.0 14 230.0 15 787.0 16 1927.0 17 3994.0 18 7234.0 19 11035.0 20 15800.0 21 22792.0 22 31460.0 23 36866.0 24 38712.0 25 40226.0 26 44185.0 27 53705.0 28 66658.0 29 83319.0 30 103566.0 31 128065.0 32 160242.0 33 203467.0 34 279551.0 35 388382.0 36 582270.0 37 1009703.0 38 870821.0 39 340638.0 40 814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.45914718256317 21.476869966850565 13.650704428254551 24.41327842233171 2 14.805291039424306 20.210220546875657 40.43321092266653 24.551277491033506 3 17.847704606781438 26.653549586935704 29.89874545280215 25.600000353480702 4 12.15952112195497 17.266626948734697 39.12213352473369 31.45171840457664 5 12.561660916655473 38.77201323445199 35.12889362950604 13.537432219386492 6 29.378028822192455 39.0726899652536 17.84102196128056 13.708259251273386 7 25.937684676554372 32.52596103607758 23.896524794570634 17.639829492797407 8 26.209673143471175 34.92680456828569 19.70384702393366 19.159675264309474 9 26.177794292824142 14.343759610048531 21.580612179974338 37.89783391715299 10-11 25.61096711993694 25.90427242874867 27.418871307532157 21.065889143782233 12-13 25.95901656728183 23.772609019925277 27.861695716260115 22.406678696532783 14-15 23.262098692726198 24.692491778515205 25.73122303900368 26.31418648975492 16-17 21.676406252865153 27.162067730609035 27.049416828882638 24.112109187643174 18-19 21.111188924275467 27.184960517060375 29.665302997097985 22.03854756156617 20-21 22.706632703782827 26.31500869323165 28.91496981117903 22.06338879180649 22-23 23.16862459198383 25.928454260765825 28.626182625553 22.276738521697347 24-25 22.609439674522015 26.023116695592545 29.41558533369623 21.951858296189208 26-27 22.551772565342638 26.292716598426004 28.388097261363598 22.76741357486776 28-29 21.689548155400892 26.800657990081543 28.989738677374543 22.52005517714302 30-31 22.54758189086873 26.344556249933724 28.858257749012928 22.249604110184617 32-33 23.07277656615071 26.618442633091437 27.928424800376096 22.380356000381752 34-35 22.902909924185227 26.45631247594026 28.798567544296162 21.842210055578352 36-37 22.680255790772232 26.039034028850473 28.512793073342124 22.767917107035174 38-39 22.782685652882396 26.010214929287717 29.156102977303494 22.05099644052639 40-41 22.313618698447264 25.333494900633347 29.63912352906284 22.71376287185655 42-43 22.540533160542665 25.595114878473975 28.64985124199184 23.214500718991516 44-45 22.656175439278634 26.524142651118567 28.335890858126522 22.483791051476278 46-47 22.752452661121854 26.286774452381877 27.649821761214817 23.310951125281456 48-49 23.620401637958068 26.082651909388062 27.602350563278673 22.6945958893752 50-51 23.037318800878026 26.14903042342537 27.258670693902943 23.554980081793666 52-53 22.347361500422398 26.749791009059514 27.170976239541616 23.731871250976468 54-55 22.469369515555687 26.69268090512848 26.987423272595763 23.850526306720067 56-57 23.618137467321667 26.05772197268601 26.786186306730674 23.53795425326165 58-59 22.35261226405497 26.466227969065315 27.757069500317517 23.4240902665622 60-61 22.56448622989593 27.10686093852901 26.96102007087581 23.36763276069925 62-63 23.01174723495707 26.30286852216453 26.526153029087297 24.159231213791106 64-65 22.095357308232032 26.529466838925863 27.00587118735132 24.36930466549078 66-67 22.807472950474892 26.09716929945176 27.011504664076885 24.08385308599646 68-69 22.324982486263508 26.00887627757488 27.370164723015645 24.295976513145963 70-71 22.323210744249888 25.72098565838289 27.448211931707593 24.50759166565963 72-73 22.911128922225323 25.775155351405232 27.79102319843624 23.52269252793321 74-75 23.0117598508164 26.238798472021337 27.247104442436026 23.502337234726234 76-77 22.722471587162126 26.215003268611365 26.750386124739222 24.312139019487287 78-79 22.415687127008056 26.284083125363072 27.037216597369017 24.263013150259855 80-81 22.032697246692994 26.70305708210212 27.583076965562952 23.68116870564193 82-83 22.913697991573933 25.551361669129975 28.570915710615207 22.964024628680882 84-85 23.232948651810382 26.137988248563104 27.020980921313537 23.608082178312976 86-87 21.788005295001465 26.810844214256445 27.54373993558109 23.857410555160996 88-89 22.722348831737243 25.58868269274319 27.3680256906233 24.320942784896264 90-91 22.365422609542883 25.5671662153183 27.650622672950746 24.41678850218807 92-93 22.726593339146245 25.936641478489964 27.189499465492773 24.14726571687102 94-95 21.431266700468026 25.86821445969188 27.986167952996226 24.71435088684387 96-97 21.817611414185656 25.75240580187228 27.562461371573566 24.867521412368497 98-99 21.193216055284278 26.48367446918621 27.424527869415638 24.89858160611388 100 22.047743415623845 25.651074717711374 26.64701163647363 25.654170230191152 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 153.0 2 116.0 3 186.0 4 128.5 5 94.5 6 198.5 7 518.5 8 876.0 9 985.0 10 818.0 11 580.0 12 571.5 13 692.0 14 877.0 15 1228.5 16 1577.0 17 2053.0 18 2830.0 19 3753.0 20 4797.5 21 5785.5 22 6904.5 23 8134.5 24 9263.5 25 10515.5 26 12506.0 27 14721.0 28 18496.5 29 27437.5 30 39111.0 31 49148.0 32 64039.5 33 78054.0 34 86877.0 35 98607.5 36 110415.0 37 132426.5 38 142877.5 39 133761.0 40 124788.5 41 114440.5 42 113080.0 43 120843.5 44 132802.0 45 160977.5 46 187834.5 47 204307.5 48 215377.5 49 209595.0 50 198905.0 51 202894.5 52 205816.0 53 198744.0 54 191059.5 55 166592.0 56 144729.0 57 120283.5 58 95067.5 59 92091.0 60 76868.0 61 52819.0 62 38758.0 63 31306.0 64 22750.0 65 11087.0 66 5990.0 67 4172.5 68 3005.5 69 2162.5 70 1514.5 71 1032.5 72 774.0 73 627.0 74 457.0 75 294.0 76 186.0 77 102.5 78 60.5 79 35.5 80 19.5 81 8.0 82 5.0 83 3.5 84 2.5 85 1.0 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014205198174665172 2 0.004816070298720074 3 0.0021650224278649878 4 0.028012739168702085 5 0.003181257445026104 6 0.0 7 3.7556511503780395E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.009135069121654819 16-17 0.003070797117073809 18-19 0.0 20-21 4.418413118091811E-5 22-23 8.174064268469851E-4 24-25 0.0 26-27 1.656904919284429E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 4.307952790139516E-4 36-37 0.0 38-39 0.0 40-41 4.0870321342349254E-4 42-43 7.290381644851488E-4 44-45 0.0 46-47 0.0 48-49 4.970714757853287E-4 50-51 0.0 52-53 0.0 54-55 3.645190822425744E-4 56-57 7.73222295666067E-5 58-59 1.656904919284429E-4 60-61 6.627619677137716E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014359842633798385 70-71 7.73222295666067E-4 72-73 0.0 74-75 1.988285903141315E-4 76-77 0.002761508198807382 78-79 5.191635413757877E-4 80-81 0.0030266129858928905 82-83 0.0018115493784176425 84-85 0.005677660856747977 86-87 0.007875821382998653 88-89 0.007665946759889293 90-91 0.005732891020724125 92-93 0.00840603095716967 94-95 0.0014801683945607567 96-97 0.004738748069153467 98-99 0.0019772398703460856 100 0.08474516360500094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4526512.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.32165206576376 #Duplication Level Percentage of deduplicated Percentage of total 1 65.61091465154742 14.645440085683633 2 15.883524087042463 7.090929964982798 3 6.734280801206535 4.509608188730553 4 3.534939092020574 3.156227219430005 5 2.054339169033734 2.2928122078120623 6 1.3454161594624738 1.8019146837106486 7 0.8862302360475318 1.3847486085448888 8 0.5974008720220632 1.0667979527248286 9 0.4446243286219205 0.8932274607125085 >10 2.2361797719751166 9.097119347281057 >50 0.2459097682481474 3.909730794561026 >100 0.311517672878485 15.56036112671266 >500 0.06333995945030893 10.013208051324352 >1k 0.04822137318523962 19.050504940604192 >5k 0.0024703572328503907 3.4492928789869386 >10k+ 6.917000251981093E-4 2.0780764881978975 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 18666 0.41237049631150874 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 14246 0.3147235664016797 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 13808 0.30504724167305863 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 12442 0.2748694800764916 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 12261 0.2708708162046185 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 12249 0.270605711417533 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 10542 0.23289455545461935 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8806 0.19454272958958244 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8462 0.18694305902646455 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 8088 0.17868062649563282 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7177 0.15855475474272465 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7082 0.15645600851163102 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6920 0.15287709388597667 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6735 0.14879006175174173 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 6567 0.1450785947325446 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6166 0.13621967643077054 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 6072 0.1341430222652674 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 6065 0.13398837780613418 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 6058 0.13383373334700097 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 5961 0.13169080298472643 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5916 0.13069666003315578 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5892 0.13016645045898476 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5873 0.12974670121276602 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5833 0.12886301858914767 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 5767 0.12740494226017737 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5658 0.12499690711081732 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5441 0.12020292887768773 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5400 0.11929715418847889 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5319 0.11750769687565173 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 5079 0.11220560113394155 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5042 0.11138819470709455 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 5002 0.1105045120834762 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 4826 0.10661630853955541 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 4821 0.10650584821160311 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4802 0.10608609896538437 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4795 0.10593145450625116 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 4703 0.10389898447192894 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 4642 0.10255136847091093 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 4610 0.10184442237201624 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4593 0.10146885725697845 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.627619677137716E-5 0.0 10-11 0.0 0.0 0.0 1.1046032795229528E-4 0.0 12-13 0.0 0.0 0.0 1.1046032795229528E-4 0.0 14-15 0.0 0.0 0.0 1.1046032795229528E-4 0.0 16-17 0.0 0.0 0.0 1.3255239354275432E-4 0.0 18-19 0.0 0.0 0.0 1.546444591332134E-4 0.0 20-21 0.0 0.0 0.0 1.7673652472367243E-4 0.0 22-23 0.0 0.0 0.0 3.092889182664268E-4 0.0 24-25 0.0 0.0 0.0 4.860254429900992E-4 0.0 26-27 0.0 0.0 0.0 8.726365908231327E-4 0.0 28-29 0.0 0.0 0.0 0.002120838296684069 0.0 30-31 0.0 0.0 0.0 0.006738080005090012 0.0 32-33 0.0 0.0 0.0 0.018590473194371293 0.0 34-35 0.0 0.0 0.0 0.03740186704464718 0.0 36-37 0.0 0.0 0.0 0.07143469408674935 0.0 38-39 0.0 0.0 0.0 0.12368242920818502 0.0 40-41 0.0 0.0 0.0 0.18214908079333492 0.0 42-43 0.0 0.0 0.0 0.24619397894007572 0.0 44-45 0.0 0.0 0.0 0.3232179656212112 0.0 46-47 0.0 0.0 0.0 0.422731675073434 0.0 48-49 0.0 0.0 0.0 0.5366494112906361 0.0 50-51 0.0 0.0 0.0 0.662298034336372 0.0 52-53 0.0 0.0 0.0 0.7861019699053046 0.0 54-55 0.0 0.0 0.0 0.9209408922366714 0.0 56-57 0.0 0.0 0.0 1.0893597542655362 0.0 58-59 0.0 0.0 0.0 1.2952246674702288 0.0 60-61 0.0 0.0 0.0 1.5117710943879086 0.0 62-63 0.0 0.0 0.0 1.7405123415115216 0.0 64-65 0.0 0.0 0.0 1.978863637166984 0.0 66-67 0.0 0.0 0.0 2.2511593916021875 0.0 68-69 0.0 0.0 0.0 2.5611442099347137 0.0 70-71 0.0 0.0 0.0 2.911679014658527 0.0 72-73 0.0 0.0 0.0 3.2750934936215788 0.0 74-75 0.0 0.0 0.0 3.6286107272001047 0.0 76-77 0.0 0.0 0.0 4.043996790464711 0.0 78-79 0.0 0.0 0.0 4.4912175202451685 0.0 80-81 0.0 0.0 0.0 4.982633427239341 0.0 82-83 0.0 0.0 0.0 5.4989470921539585 0.0 84-85 0.0 0.0 0.0 5.995223253578031 0.0 86-87 0.0 0.0 0.0 6.524052073649645 0.0 88 0.0 0.0 0.0 6.9530137112195884 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9345 0.0 43.017845 1 GGGCACC 4790 0.0 40.165764 94 GTATCAA 15825 0.0 38.149 1 GTACTAG 4855 0.0 33.217484 1 GTGGTAT 2525 0.0 32.400352 1 CTGTGCG 6430 0.0 32.38574 9 TAGTACT 6490 0.0 31.942886 4 CAACGCA 18300 0.0 31.61416 5 ATCAACG 18660 0.0 30.987957 3 ACTGTGC 7525 0.0 29.477852 8 TATCAAC 20425 0.0 29.439928 2 TACCCCG 370 0.0 29.214659 5 TAGGCAT 6090 0.0 29.170858 5 GTACTGT 7245 0.0 29.06029 6 TACTGTG 8190 0.0 28.576284 7 CTAGTAC 7635 0.0 28.569876 3 AGGGCTA 5805 0.0 28.336126 5 TATTCTC 2575 0.0 28.107237 5 GCTATTG 5785 0.0 27.864485 8 CTATTGA 5810 0.0 27.83193 9 >>END_MODULE