FastQCFastQC Report
Mon 16 Jan 2017
ERR1146440_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146440_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5045364
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC191610.37977438297811617No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT157990.3131389529080558No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157060.3112956765854753No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA130330.25831634744291987No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC115070.22807075961219053No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA104010.2061496454963408No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC99610.19742876827122882No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC85880.17021566729377702No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT79880.1583235619868061No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG79670.15790733830106213No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC76760.15213966722718125No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT72510.1437160926347435No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT64340.12752300924175144No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC63900.12665092151924023No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG63100.1250653074783108No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT60950.12080396974331288No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC59910.11874267149010458No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT58410.11576964516336184No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG55340.10968485128129506No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG54610.10823797846894692No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA54580.10817851794241208No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT54250.10752445215052868No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT53870.1067712854810872No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA53230.10550279424834362No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT52750.10455142582378595No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA52670.10439286441969302No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC52260.10358023722371666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC52650.040.3478794
GTACTAG51650.034.7933041
CAACGCA90600.032.3568155
GTATCAA104900.031.519811
GCATCGT55250.030.9330738
TAGGCAT64300.030.1020225
ATCAACG97900.030.0931663
CTGTGCG74300.029.574659
TAGTACT75950.029.5076524
CTAGGCA73050.027.2702874
GTACTGT81650.026.96646
CTAGTAC85800.026.9439243
ACTGTGC85650.026.532128
TATCAAC124250.026.4734572
TACCCCG3200.026.4260315
AACGCAG119150.026.2413736
GGTATCA68800.026.1202621
TACTGTG92600.025.8590837
AGGGCTA58900.025.6034875
ACGCAGA115150.025.2802687
Produced by FastQC (version 0.11.5)