##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146440_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5045364
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.532022070161837	31.0	31.0	34.0	27.0	34.0
2	30.631868979126185	31.0	31.0	34.0	27.0	34.0
3	30.81913931284244	31.0	31.0	34.0	27.0	34.0
4	34.30894044512943	37.0	35.0	37.0	30.0	37.0
5	34.520638946962	37.0	35.0	37.0	30.0	37.0
6	34.72853356071039	37.0	35.0	37.0	31.0	37.0
7	34.63663097449461	37.0	35.0	37.0	31.0	37.0
8	34.57259852807449	37.0	35.0	37.0	30.0	37.0
9	36.00161911013755	39.0	35.0	39.0	30.0	39.0
10-11	35.923652386626614	39.0	35.0	39.0	30.0	39.0
12-13	35.912030529412746	39.0	35.0	39.0	30.0	39.0
14-15	36.951619546181405	40.0	36.0	41.0	30.0	41.0
16-17	36.78015966736989	40.0	36.0	41.0	29.5	41.0
18-19	36.728034191388375	40.0	36.0	41.0	29.5	41.0
20-21	37.060182080024354	40.0	36.0	41.0	30.0	41.0
22-23	36.77112711788486	40.0	36.0	41.0	29.5	41.0
24-25	36.882354276123586	40.0	36.0	41.0	30.0	41.0
26-27	36.954419641476804	40.0	36.0	41.0	30.0	41.0
28-29	36.78884764310365	40.0	36.0	41.0	29.5	41.0
30-31	36.73408033592819	40.0	36.0	41.0	30.0	41.0
32-33	36.84099353386594	40.0	36.0	41.0	30.0	41.0
34-35	36.888612992045765	40.0	36.0	41.0	30.0	41.0
36-37	36.82372728706987	40.0	35.5	41.0	30.0	41.0
38-39	36.65583325603465	40.0	35.0	41.0	30.0	41.0
40-41	36.61742908935807	39.0	35.0	41.0	30.0	41.0
42-43	36.46245424512483	39.0	35.0	41.0	29.0	41.0
44-45	36.284002303897196	39.0	35.0	41.0	28.5	41.0
46-47	36.156083485750486	39.0	35.0	41.0	28.5	41.0
48-49	36.03061037816102	39.0	35.0	41.0	28.0	41.0
50-51	35.813250738697946	38.0	34.0	40.0	28.0	41.0
52-53	35.62411651567657	38.0	34.0	40.0	27.5	41.0
54-55	35.42585579553824	38.0	34.0	40.0	27.0	41.0
56-57	35.275130595136446	37.5	34.0	40.0	27.0	41.0
58-59	35.01345443064167	37.0	33.5	40.0	27.0	41.0
60-61	34.763840725862394	36.5	33.0	40.0	26.5	41.0
62-63	34.492858097057024	36.0	33.0	39.5	26.0	41.0
64-65	34.20106131093812	35.5	33.0	39.0	26.0	41.0
66-67	33.910863319276864	35.0	33.0	39.0	26.0	41.0
68-69	33.61930744739131	35.0	32.0	38.0	26.0	40.0
70-71	33.5516733777781	35.0	32.0	37.5	26.0	40.0
72-73	33.33961117572488	35.0	32.0	37.0	26.0	40.0
74-75	33.073400353274806	35.0	32.0	36.5	25.5	39.0
76-77	32.84127448485382	35.0	32.0	36.0	25.0	39.0
78-79	32.62187554753235	35.0	32.0	36.0	25.0	39.0
80-81	32.40590094193402	35.0	32.0	35.5	25.0	37.5
82-83	32.179019392852524	35.0	31.5	35.0	25.0	37.0
84-85	32.006336014606674	35.0	31.0	35.0	24.5	37.0
86-87	31.8422955211953	35.0	31.5	35.0	24.0	36.0
88-89	31.847923460031822	35.0	32.0	35.0	24.5	36.0
90-91	31.77665139720345	35.0	32.0	35.0	24.0	36.0
92-93	31.64010178452932	35.0	32.0	35.0	24.0	36.0
94-95	31.626079406758365	35.0	32.0	35.0	24.0	35.0
96-97	31.547626097938625	35.0	32.0	35.0	24.0	35.0
98-99	31.474010299356003	35.0	32.0	35.0	24.0	35.0
100	31.000496098993057	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7241216960402355
1101	2	0.8623605397932899
1101	3	0.8008855672651585
1101	4	0.8959273639100473
1101	5	0.8510064132869957
1101	6	0.5845445964778193
1101	7	0.6380309358886578
1101	8	0.652622712522593
1101	9	0.8605586002465486
1101	10-11	0.8423975194496123
1101	12-13	0.6998033737014708
1101	14-15	0.986446448183564
1101	16-17	1.124643514897258
1101	18-19	1.2529186797129555
1101	20-21	0.8729563626131309
1101	22-23	1.201274302969459
1101	24-25	1.0975046855974426
1101	26-27	0.9819889504802362
1101	28-29	1.1005050599757524
1101	30-31	1.1200190607062908
1101	32-33	0.9594416793031826
1101	34-35	0.9072795009819288
1101	36-37	0.8306725383129958
1101	38-39	0.9589190080080883
1101	40-41	0.8837259697581246
1101	42-43	0.9141670498422911
1101	44-45	0.8392360042118767
1101	46-47	0.9256182450567749
1101	48-49	0.9306281124572706
1101	50-51	0.9788887541219893
1101	52-53	1.0286180493656403
1101	54-55	1.0205484596999383
1101	56-57	0.9016108741896858
1101	58-59	0.7912457683903114
1101	60-61	0.8825769588577472
1101	62-63	0.8464181983199666
1101	64-65	0.806396842961135
1101	66-67	0.8679938784702017
1101	68-69	0.9004501506974592
1101	70-71	0.7926870560657946
1101	72-73	0.7870342448718475
1101	74-75	0.7524081373402112
1101	76-77	0.7346189078950545
1101	78-79	0.6909253570157787
1101	80-81	0.7798438184139798
1101	82-83	0.7298996361838448
1101	84-85	0.7332515186389443
1101	86-87	0.7546740744642548
1101	88-89	0.729598563876948
1101	90-91	0.7822117985804873
1101	92-93	0.8148752614474262
1101	94-95	0.7286654698918476
1101	96-97	0.7653155254183588
1101	98-99	0.791426247108749
1101	100	0.8996157086598444
1102	1	0.6666161570993445
1102	2	0.8275396493602685
1102	3	0.8024277520885477
1102	4	0.8865166957530661
1102	5	0.8243214673003081
1102	6	0.5883610270895332
1102	7	0.6323768484386534
1102	8	0.6552556530062574
1102	9	0.8515467965434453
1102	10-11	0.8048630538979609
1102	12-13	0.7641768199058703
1102	14-15	0.9858940971396777
1102	16-17	1.1450551908396278
1102	18-19	1.1908012494442417
1102	20-21	0.8955663631628923
1102	22-23	1.18780164926617
1102	24-25	1.0687090397159622
1102	26-27	0.9241016892179488
1102	28-29	1.1611081982760822
1102	30-31	1.1534163446774812
1102	32-33	0.9925791499305205
1102	34-35	0.9035975598011134
1102	36-37	0.8347402828738026
1102	38-39	0.8996677793902208
1102	40-41	0.8519065292813721
1102	42-43	0.9194870722914885
1102	44-45	0.9955576482916229
1102	46-47	0.9166352954497441
1102	48-49	0.8758105071077722
1102	50-51	0.974055170247631
1102	52-53	0.9102263793795942
1102	54-55	0.8848103850759301
1102	56-57	0.8489174092461482
1102	58-59	0.8094994496906622
1102	60-61	0.8174897152688061
1102	62-63	0.7475678932355407
1102	64-65	0.7201827347753067
1102	66-67	0.7705787737640648
1102	68-69	0.7621046759337489
1102	70-71	0.7432718279160682
1102	72-73	0.6765554355156311
1102	74-75	0.7129197553720132
1102	76-77	0.6701171444392884
1102	78-79	0.7578724587390298
1102	80-81	0.7382725956782323
1102	82-83	0.7119432229014393
1102	84-85	0.6822518026266593
1102	86-87	0.7167420008176748
1102	88-89	0.7065909973919418
1102	90-91	0.7216175222482981
1102	92-93	0.7964690632300702
1102	94-95	0.748217518060482
1102	96-97	0.7466009458871241
1102	98-99	0.7281987098792051
1102	100	0.7859607227023986
1103	1	0.6075471953338507
1103	2	0.7785037506412387
1103	3	0.7015544311950848
1103	4	0.6593416427089025
1103	5	0.7120867689572776
1103	6	0.551460395596834
1103	7	0.5674495075136505
1103	8	0.6373151508659021
1103	9	0.6981804943289589
1103	10-11	0.7728497967182761
1103	12-13	0.6150111335066129
1103	14-15	0.7708408024079674
1103	16-17	1.000895014894084
1103	18-19	1.0900079933793094
1103	20-21	0.7693890799566887
1103	22-23	1.047183288498232
1103	24-25	0.9802669746988144
1103	26-27	0.8248691300482989
1103	28-29	1.0621305580650215
1103	30-31	1.0402578943234815
1103	32-33	0.8811237465505428
1103	34-35	0.8281646923040711
1103	36-37	0.8170596510696058
1103	38-39	0.8877348460341921
1103	40-41	0.7769134834951998
1103	42-43	0.8190803235136457
1103	44-45	0.8600135905208646
1103	46-47	0.827514421691582
1103	48-49	0.8319441788073973
1103	50-51	0.8294535842606265
1103	52-53	0.8260866886849385
1103	54-55	0.8274689273343014
1103	56-57	0.7682269081228128
1103	58-59	0.7710717436684149
1103	60-61	0.7520830145460593
1103	62-63	0.6844282265117059
1103	64-65	0.6890825975159913
1103	66-67	0.7542146492318764
1103	68-69	0.7764442299963079
1103	70-71	0.7374585865192032
1103	72-73	0.6816860432120535
1103	74-75	0.7054197311823884
1103	76-77	0.6671633357351254
1103	78-79	0.7040791289787904
1103	80-81	0.7066369397890213
1103	82-83	0.6588582561767282
1103	84-85	0.6276978002693454
1103	86-87	0.6529848948818113
1103	88-89	0.6475962090450196
1103	90-91	0.6692727013786843
1103	92-93	0.6848360666601039
1103	94-95	0.6523544969237385
1103	96-97	0.6604226085480462
1103	98-99	0.6753300559548698
1103	100	0.682984419671218
1104	1	0.5314570821511388
1104	2	0.6948666420440439
1104	3	0.6299703364745639
1104	4	0.7380967874943778
1104	5	0.6595832802819643
1104	6	0.3495759623137573
1104	7	0.4363217715631862
1104	8	0.44961959062712964
1104	9	0.5485226116552226
1104	10-11	0.6152328561549822
1104	12-13	0.4919707943588634
1104	14-15	0.6482512656234505
1104	16-17	0.7868914131592533
1104	18-19	0.775237394795262
1104	20-21	0.6491039729877528
1104	22-23	0.9109746116872657
1104	24-25	0.7208779417157203
1104	26-27	0.6205302721983728
1104	28-29	0.7959290395512326
1104	30-31	0.8715190526945875
1104	32-33	0.7255912124876716
1104	34-35	0.6412658663905688
1104	36-37	0.5950866688290617
1104	38-39	0.6974653370435888
1104	40-41	0.6262532162409826
1104	42-43	0.6102270254867079
1104	44-45	0.6526687339075394
1104	46-47	0.559575968610126
1104	48-49	0.6000000546554674
1104	50-51	0.7354570525954855
1104	52-53	0.6661976657116568
1104	54-55	0.6627542697904261
1104	56-57	0.5840339551186133
1104	58-59	0.5519658339794873
1104	60-61	0.6171117924278704
1104	62-63	0.5618903749941495
1104	64-65	0.544554236966249
1104	66-67	0.6508121368851647
1104	68-69	0.5842977132688958
1104	70-71	0.47810788436955676
1104	72-73	0.47174650438708454
1104	74-75	0.5070610367772588
1104	76-77	0.5303599174236524
1104	78-79	0.4563356432473924
1104	80-81	0.4264005533225941
1104	82-83	0.5276859011790833
1104	84-85	0.525365928496548
1104	86-87	0.46326169050048804
1104	88-89	0.38835983367921756
1104	90-91	0.4315194868823262
1104	92-93	0.44209073232219964
1104	94-95	0.3717500975610797
1104	96-97	0.4079388122481795
1104	98-99	0.4346231054510703
1104	100	0.4921219577919125
1105	1	0.49419531509613535
1105	2	0.6363041988481442
1105	3	0.5636420845260126
1105	4	0.7272549507596864
1105	5	0.5353466929344748
1105	6	0.33496439906497955
1105	7	0.4268268804893509
1105	8	0.45242278680438375
1105	9	0.46453861319754
1105	10-11	0.54112975843816
1105	12-13	0.5128790058215813
1105	14-15	0.6741543647173458
1105	16-17	0.7799699198655787
1105	18-19	0.7990421849299381
1105	20-21	0.6313664630040066
1105	22-23	0.8800525262538983
1105	24-25	0.7403113982967326
1105	26-27	0.5735179956278245
1105	28-29	0.827315885787705
1105	30-31	0.8377920497476552
1105	32-33	0.6734751737645013
1105	34-35	0.5327799365935562
1105	36-37	0.523675310763565
1105	38-39	0.6790384318685554
1105	40-41	0.5378329037840146
1105	42-43	0.5523711908451574
1105	44-45	0.6037530897146439
1105	46-47	0.6118464972669315
1105	48-49	0.6292829975897263
1105	50-51	0.6095425926819686
1105	52-53	0.6515658767612607
1105	54-55	0.6197845515387499
1105	56-57	0.5565980060426341
1105	58-59	0.50096436051561
1105	60-61	0.5415868709208098
1105	62-63	0.5974897615098769
1105	64-65	0.46909902957965244
1105	66-67	0.5376958124201536
1105	68-69	0.4585011648924606
1105	70-71	0.45752070212824236
1105	72-73	0.44400630911869143
1105	74-75	0.4928346225273401
1105	76-77	0.47592911170055174
1105	78-79	0.520597861292508
1105	80-81	0.6169486095896275
1105	82-83	0.5484069173183386
1105	84-85	0.5111124031997889
1105	86-87	0.516280570117118
1105	88-89	0.5362097124971399
1105	90-91	0.5463115322429779
1105	92-93	0.5803609442531226
1105	94-95	0.4781002793341962
1105	96-97	0.4939410775267561
1105	98-99	0.5013920194944212
1105	100	0.5362656274055055
1106	1	0.5159045856756279
1106	2	0.5771217814994216
1106	3	0.5733969326162303
1106	4	0.7622965789880851
1106	5	0.5976079204623304
1106	6	0.2878392308275721
1106	7	0.4454864556245468
1106	8	0.46893760893087943
1106	9	0.6243983230200314
1106	10-11	0.5722766092657565
1106	12-13	0.45997719180806484
1106	14-15	0.6966061097221186
1106	16-17	0.7241281749055943
1106	18-19	0.7779809849783135
1106	20-21	0.6414044737996321
1106	22-23	0.9084598168561513
1106	24-25	0.6359461014637304
1106	26-27	0.5732844204766536
1106	28-29	0.8075103926649163
1106	30-31	0.7950988939304651
1106	32-33	0.7051911738400563
1106	34-35	0.5608448612837691
1106	36-37	0.4449809089525658
1106	38-39	0.6491650098823172
1106	40-41	0.6048142481525147
1106	42-43	0.6330750449154863
1106	44-45	0.6148713886273427
1106	46-47	0.6002715400675385
1106	48-49	0.6037041693964653
1106	50-51	0.6275478652728097
1106	52-53	0.5895831623448657
1106	54-55	0.5708527604489433
1106	56-57	0.5591568650332519
1106	58-59	0.5530760113366568
1106	60-61	0.5255975989702932
1106	62-63	0.5500643901704336
1106	64-65	0.6060738031711992
1106	66-67	0.5938013183767978
1106	68-69	0.5753719949856801
1106	70-71	0.5714451502676923
1106	72-73	0.5362070266849273
1106	74-75	0.4919341836251476
1106	76-77	0.5059536333181853
1106	78-79	0.5186830319214053
1106	80-81	0.5652956862462943
1106	82-83	0.5774509345129673
1106	84-85	0.5527245162537682
1106	86-87	0.583422921890488
1106	88-89	0.5881688274247807
1106	90-91	0.5889072169913554
1106	92-93	0.6451483375534579
1106	94-95	0.5865284205657808
1106	96-97	0.6339154964833114
1106	98-99	0.6085173980590035
1106	100	0.5405091730388101
1107	1	0.3897244562683255
1107	2	0.5522996332572667
1107	3	0.439050916460733
1107	4	0.5813954356405375
1107	5	0.46963652641016296
1107	6	0.25342960006420867
1107	7	0.21523623011864146
1107	8	0.2692223952017514
1107	9	0.3631332657993127
1107	10-11	0.40108458015039616
1107	12-13	0.25313367334377546
1107	14-15	0.44079852495010385
1107	16-17	0.5814348067998765
1107	18-19	0.6572806580710946
1107	20-21	0.4340333822273479
1107	22-23	0.6462234339621418
1107	24-25	0.38040678503695347
1107	26-27	0.36004615919135574
1107	28-29	0.5149054397269595
1107	30-31	0.5147148703862925
1107	32-33	0.40839207702829583
1107	34-35	0.32348751697484346
1107	36-37	0.30392957289036104
1107	38-39	0.37308804294526254
1107	40-41	0.20922854266152768
1107	42-43	0.31148652956233036
1107	44-45	0.2618835622612181
1107	46-47	0.27073691143657186
1107	48-49	0.2121902580348376
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2209	26-27	0.40481795885374083
2209	28-29	0.5130940869013116
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2210	2	-0.09020149760941365
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2210	4	-0.03640316756838757
2210	5	-0.06496813475530416
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2210	18-19	0.18634574262635084
2210	20-21	-0.47083873702346324
2210	22-23	-1.7071250961833968
2210	24-25	-0.836870514173846
2210	26-27	-0.24657868489939005
2210	28-29	0.040124294677688965
2210	30-31	-0.04326544216862516
2210	32-33	-0.10977030752594885
2210	34-35	-0.046610786328542986
2210	36-37	-0.04735009664640444
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2211	26-27	-0.5818493078560607
2211	28-29	-0.24176543618318647
2211	30-31	-0.11117947044454723
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2211	34-35	-0.3007453648138352
2211	36-37	-0.3134917998943152
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2213	82-83	-0.5027613461196552
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2214	1	-0.8814218477234057
2214	2	-0.8343887435709902
2214	3	-1.0833654126798962
2214	4	-0.7703315126005847
2214	5	-1.0865196461937714
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2214	8	-0.8953299895198228
2214	9	-4.613204117322972
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2214	14-15	-3.616726565052474
2214	16-17	-3.1014010698883467
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2214	28-29	-0.9421714794337888
2214	30-31	-1.0591164228056513
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2214	34-35	-1.0410515725058076
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2214	38-39	-1.0078654013646329
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2214	44-45	-1.6631514581918907
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2214	50-51	-1.1549229810594
2214	52-53	-1.1143471123660191
2214	54-55	-1.0070527040187471
2214	56-57	-0.9663252420790371
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2214	88-89	-0.8940668378190963
2214	90-91	-0.9003279081960649
2214	92-93	-1.004599280340038
2214	94-95	-1.030711905321244
2214	96-97	-0.9275770905191862
2214	98-99	-1.1329730275690402
2214	100	-1.0178141479044278
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2215	2	-2.373453196012637
2215	3	-3.9446754125132912
2215	4	-2.1466451153396306
2215	5	-1.6386201610135842
2215	6	-1.4390120943664044
2215	7	-1.4100851110279038
2215	8	-1.2882346603850152
2215	9	-1.8990988458346578
2215	10-11	-1.562272204573759
2215	12-13	-1.5497802177868891
2215	14-15	-3.805443066360354
2215	16-17	-3.689449679958159
2215	18-19	-2.4638187792938098
2215	20-21	-1.9891804399677113
2215	22-23	-3.3714773113896612
2215	24-25	-5.5646736777174794
2215	26-27	-3.646423169097176
2215	28-29	-1.983549705388036
2215	30-31	-4.133731453378282
2215	32-33	-4.101395123544712
2215	34-35	-3.1134785668077996
2215	36-37	-2.100637015381473
2215	38-39	-1.9391743390697727
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2215	44-45	-3.4818833915985365
2215	46-47	-2.905315225721857
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2215	64-65	-2.022816714450755
2215	66-67	-1.8345117497545118
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2215	70-71	-1.7070515285859074
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2215	80-81	-1.8332780904021035
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2215	84-85	-1.5563648407342185
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2215	90-91	-1.8039941770852863
2215	92-93	-4.006669389135293
2215	94-95	-2.160069234422725
2215	96-97	-1.74394630920931
2215	98-99	-3.272930032200751
2215	100	-3.3402414025319764
2216	1	-0.46934238880090007
2216	2	-0.4651106002003118
2216	3	-0.6445897216461987
2216	4	-0.6662065395466783
2216	5	-0.7604424580255795
2216	6	-0.6437558289979322
2216	7	-0.8214090349244785
2216	8	-0.7268717719818483
2216	9	-0.7572416515275151
2216	10-11	-0.8512634895642037
2216	12-13	-1.0160293484592557
2216	14-15	-1.4635004140417252
2216	16-17	-1.1599352304909303
2216	18-19	-3.116600782969819
2216	20-21	-1.3404679762931906
2216	22-23	-0.8205956424047827
2216	24-25	-0.9848922980945218
2216	26-27	-0.9225565550041352
2216	28-29	-0.9428659481539867
2216	30-31	-1.1070698733132147
2216	32-33	-1.1101066402730595
2216	34-35	-1.2856706385397558
2216	36-37	-1.302540687669783
2216	38-39	-1.3252008287247818
2216	40-41	-1.3636893615638428
2216	42-43	-1.3263291330786586
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2216	50-51	-1.2525823186322498
2216	52-53	-1.277082198797423
2216	54-55	-1.1396863675509223
2216	56-57	-1.2174779764032024
2216	58-59	-1.2501953766213418
2216	60-61	-1.2942629716117722
2216	62-63	-1.2926598566195793
2216	64-65	-1.2887851764859803
2216	66-67	-1.2706538134141354
2216	68-69	-1.349917082066547
2216	70-71	-1.360765342823008
2216	72-73	-1.413352443726474
2216	74-75	-1.4510509543556047
2216	76-77	-1.309853393845323
2216	78-79	-1.3762368990391884
2216	80-81	-1.4900822867708001
2216	82-83	-1.5736190993176749
2216	84-85	-1.4035393706763486
2216	86-87	-1.382704318788896
2216	88-89	-1.247831983463925
2216	90-91	-1.3217408798497452
2216	92-93	-1.2944079109057398
2216	94-95	-1.183437459876636
2216	96-97	-1.1394636057245506
2216	98-99	-1.1386472430251224
2216	100	-1.1673111784938257
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	21082.0
1	3355.0
2	537.0
3	551.0
4	501.0
5	270.0
6	238.0
7	325.0
8	627.0
9	1072.0
10	1858.0
11	3097.0
12	5162.0
13	7390.0
14	9829.0
15	12346.0
16	15002.0
17	17876.0
18	20955.0
19	24131.0
20	27861.0
21	32084.0
22	36909.0
23	42839.0
24	48186.0
25	54957.0
26	62695.0
27	72343.0
28	86145.0
29	102086.0
30	122546.0
31	148059.0
32	184246.0
33	235227.0
34	326856.0
35	445934.0
36	664920.0
37	1038975.0
38	874990.0
39	290991.0
40	311.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77075169719088	20.88195463853458	12.434080441682063	23.913213222592482
2	15.401850290346056	19.771207507085677	40.956086216800344	23.870855985767918
3	18.698412805903185	26.63374593461889	28.74486605280051	25.922975206677414
4	12.15197642599378	16.61143375944421	40.08323868960102	31.15335112496099
5	13.170853581090899	38.533601157829985	35.083900867775256	13.211644393303857
6	30.04726770138668	38.38423775229541	18.22629897543159	13.342195570886314
7	27.082299083219453	31.586994545534232	23.735213377585293	17.595492993661026
8	26.049976532162887	35.274350570439765	20.0190611430789	18.65661175431844
9	26.092813482406395	15.029370825551124	21.556663716500303	37.32115197554218
10-11	25.641827339590368	25.817707435039093	27.66879263176784	20.871672593602693
12-13	25.953436598323183	23.539748731356692	27.598709870135124	22.908104800185004
14-15	23.268704519101803	24.748413505118013	24.998640019145345	26.98424195663484
16-17	22.36276371665368	27.383837135848328	26.302855423391396	23.950543724106595
18-19	21.772814065164653	28.080222117626054	27.87232062543018	22.274643191779113
20-21	23.496496319965246	27.1938975289416	26.811287499041008	22.49831865205214
22-23	24.0159186827297	26.747795230371114	26.522343305633566	22.71394278126562
24-25	23.38508331896138	26.87431421878704	27.20854055595392	22.532061906297667
26-27	23.256504991119485	27.158433801548586	26.171197241112758	23.41386396621917
28-29	22.416629143638154	27.68368684675064	26.794351223516376	23.105332786094827
30-31	23.177251341207167	27.283887621922037	26.755733130158166	22.78312790671263
32-33	23.593011101693328	27.453020338825002	25.81227382875123	23.14169473073044
34-35	23.55851540545078	27.234790458270254	26.70824471967196	22.49844941660701
36-37	23.20524096842994	26.861198923647837	26.364349711210195	23.56921039671203
38-39	23.439646219136918	26.922312353842244	26.957097762942468	22.68094366407837
40-41	23.03516470266427	26.097872440074717	27.628135150203708	23.238827707057304
42-43	23.02734431245814	26.32431030378351	26.94692196249124	23.701423421267105
44-45	23.192912734919478	27.194856439053105	26.778829609876937	22.833401216150484
46-47	23.12784481457103	26.93758311778971	26.334795093627566	23.599776974011693
48-49	23.779238830992714	26.563943958998586	26.619654998033376	23.037162211975325
50-51	23.393067654157722	26.45311016736949	26.62318892133561	23.530633257137172
52-53	22.924067840651944	26.963504723068226	26.46677751323742	23.645649923042402
54-55	22.862421138063134	26.877054844513133	26.41679956674822	23.84372445067551
56-57	23.970734649604577	26.316328532391537	26.23125321747389	23.481683600529998
58-59	22.808099749311502	26.750196286303	27.10830420590732	23.333399758478173
60-61	22.835430628361582	27.359580198931273	26.406868095328	23.39812107737915
62-63	23.315640037060284	26.686970122637653	25.946949998505637	24.05043984179643
64-65	22.51258085880135	26.83040074719446	26.44822481035925	24.20879358364494
66-67	23.275758654235883	26.41648735606164	26.401322839993036	23.90643114970944
68-69	22.60272068235802	26.620602783465035	26.707511947394096	24.06916458678285
70-71	22.63821214924981	26.28740506686047	26.7921441762152	24.282238607674522
72-73	23.229862978155065	26.417463753689496	27.086161923602802	23.266511344552633
74-75	23.416386041899965	26.724687749003934	26.664426119072317	23.19450009002378
76-77	23.11521317097694	26.630665767889543	26.30404543354207	23.950075627591445
78-79	22.999148119904216	26.71360644255749	26.334715130418978	23.952530307119318
80-81	22.618140610308924	27.057830606749068	26.96420623982444	23.359822543117573
82-83	23.46003295018974	26.038540722656485	27.916677626151642	22.584748701002137
84-85	23.630005985445813	26.589030243191242	26.627840483634387	23.15312328772856
86-87	22.309590978488202	27.259108593474746	27.06500515993293	23.366295268104118
88-89	23.429446378357596	25.974595015735552	26.883270284891562	23.712688321015285
90-91	23.070124199533364	26.14968419253067	26.99417568232882	23.786015925607146
92-93	23.359142442129926	26.356065491302516	26.95361826068352	23.33117380588404
94-95	22.3091263049726	26.359579666889875	27.549340275683008	23.781953752454513
96-97	22.677807379188867	26.24460706181043	27.119522160674535	23.958063398326168
98-99	22.03339524912765	26.99801186069738	26.96849188496268	24.00010100521229
100	22.772478815953907	26.26412904332519	26.181304653476218	24.782087487244684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17587.0
1	10322.0
2	2746.5
3	1947.0
4	1033.0
5	527.0
6	409.5
7	386.5
8	405.5
9	382.0
10	362.0
11	342.5
12	308.5
13	321.5
14	364.0
15	432.5
16	489.0
17	566.0
18	714.0
19	906.0
20	1139.0
21	1579.0
22	2170.5
23	2894.5
24	4045.0
25	5535.0
26	7906.5
27	10894.0
28	15592.0
29	24408.5
30	36867.5
31	49473.0
32	65072.5
33	81129.5
34	94548.0
35	108702.0
36	123232.0
37	147835.5
38	159236.0
39	151319.5
40	142777.0
41	134009.0
42	133880.5
43	143317.0
44	157295.5
45	184607.5
46	212960.0
47	231892.0
48	242492.5
49	236579.5
50	224623.5
51	229705.0
52	232209.5
53	221591.5
54	209908.0
55	183034.5
56	160925.5
57	134054.5
58	106271.0
59	100407.5
60	83549.5
61	58672.0
62	44286.0
63	36551.5
64	26931.5
65	14337.0
66	8687.5
67	6234.5
68	4604.5
69	3403.5
70	2560.5
71	1937.0
72	1485.5
73	1154.0
74	904.0
75	675.0
76	466.0
77	307.5
78	197.5
79	130.0
80	93.5
81	65.0
82	47.5
83	34.5
84	24.5
85	20.5
86	20.5
87	20.0
88	13.0
89	10.5
90	10.0
91	8.5
92	6.0
93	5.5
94	6.0
95	4.0
96	4.0
97	4.0
98	3.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0036936879083451
2	0.4540604007956611
3	0.487913260569505
4	0.47372201490318633
5	0.43949257179462176
6	0.4917583746187589
7	0.5078721773097045
8	0.5099334755629128
9	0.5240058001761617
10-11	0.5385637190894452
12-13	0.5373150480322134
14-15	0.5333807431931572
16-17	0.5389898528629451
18-19	0.5375628002261086
20-21	0.5365123309239929
22-23	0.4999639272805689
24-25	0.5333311927543781
26-27	0.5143137343509804
28-29	0.5197642826166754
30-31	0.5228165896454646
32-33	0.5250364493027659
34-35	0.5369582848730042
36-37	0.5558964625743554
38-39	0.5441728287592332
40-41	0.5502378024657884
42-43	0.5476215392982547
44-45	0.5577298288091801
46-47	0.5551928463436929
48-49	0.5274148703641601
50-51	0.5429637980530245
52-53	0.535461861621877
54-55	0.5449854559552096
56-57	0.532558205909425
58-59	0.5387916511078289
60-61	0.5311113330970768
62-63	0.5255121335150447
64-65	0.5376321708403993
66-67	0.5365321510995045
68-69	0.5560847542417158
70-71	0.5396439186548285
72-73	0.5431719098958965
74-75	0.5400105919017935
76-77	0.5290599449316244
78-79	0.5594442739909351
80-81	0.5332717322278432
82-83	0.5327663177522969
84-85	0.5422502717346063
86-87	0.8498197553238973
88-89	1.1167281488511036
90-91	1.107006352762655
92-93	1.1068874317095851
94-95	1.111574903218083
96-97	1.1047171224910632
98-99	1.100465694843821
100	1.0958178636863465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5045364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.31132257801037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70336815634685	22.037352446948766
2	13.172770214714093	7.985681744484116
3	5.398367708088425	4.908949949837482
4	2.6814844631330192	3.251173621997916
5	1.5465949377400512	2.3439669027678276
6	0.980171717340471	1.7826180663689652
7	0.6594763617309356	1.399272051309933
8	0.4702593334345016	1.1403345880842652
9	0.3298087258630982	0.8997244810811064
>10	1.6327609654200426	8.811571570542824
>50	0.15834957357124385	3.4074510351496015
>100	0.19551242736119634	13.319668994652782
>500	0.03926132774249563	8.359903837923262
>1k	0.030050267466621858	15.987018100447568
>5k	0.0013718600365196934	2.670176111306173
>10k+	3.919600104341981E-4	1.6951364970973801
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19161	0.37977438297811617	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	15799	0.3131389529080558	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15706	0.3112956765854753	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13033	0.25831634744291987	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11507	0.22807075961219053	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10401	0.2061496454963408	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9961	0.19742876827122882	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8588	0.17021566729377702	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7988	0.1583235619868061	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7967	0.15790733830106213	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7676	0.15213966722718125	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7251	0.1437160926347435	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6434	0.12752300924175144	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6390	0.12665092151924023	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6310	0.1250653074783108	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6095	0.12080396974331288	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5991	0.11874267149010458	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5841	0.11576964516336184	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5534	0.10968485128129506	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5461	0.10823797846894692	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5458	0.10817851794241208	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5425	0.10752445215052868	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5387	0.1067712854810872	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5323	0.10550279424834362	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5275	0.10455142582378595	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5267	0.10439286441969302	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5226	0.10358023722371666	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.9820175511618192E-5	0.0
3	0.0	0.0	0.0	1.9820175511618192E-5	0.0
4	0.0	0.0	0.0	1.9820175511618192E-5	0.0
5	0.0	0.0	0.0	1.9820175511618192E-5	0.0
6	0.0	0.0	0.0	1.9820175511618192E-5	0.0
7	0.0	0.0	0.0	1.9820175511618192E-5	0.0
8	0.0	0.0	0.0	1.9820175511618192E-5	0.0
9	0.0	0.0	0.0	3.9640351023236384E-5	0.0
10-11	0.0	0.0	0.0	6.937061429066367E-5	0.0
12-13	0.0	0.0	0.0	9.910087755809096E-5	0.0
14-15	0.0	0.0	0.0	9.910087755809096E-5	0.0
16-17	1.9820175511618192E-5	0.0	0.0	9.910087755809096E-5	0.0
18-19	1.9820175511618192E-5	0.0	1.9820175511618192E-5	9.910087755809096E-5	0.0
20-21	1.9820175511618192E-5	0.0	1.9820175511618192E-5	1.0901096531390005E-4	0.0
22-23	1.9820175511618192E-5	0.0	1.9820175511618192E-5	1.5856140409294554E-4	0.0
24-25	1.9820175511618192E-5	0.0	1.9820175511618192E-5	2.4775219389522736E-4	0.0
26-27	1.9820175511618192E-5	0.0	1.9820175511618192E-5	4.855943000346457E-4	0.0
28-29	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.0014666929878597462	0.0
30-31	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.005728030722857658	0.0
32-33	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.015578657952131897	0.0
34-35	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.03097893432465923	0.0
36-37	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.060610096714528425	0.0
38-39	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.106781195568843	0.0
40-41	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.16035512997674695	1.9820175511618192E-5
42-43	1.9820175511618192E-5	0.0	1.9820175511618192E-5	0.21754624641552125	1.9820175511618192E-5
44-45	1.9820175511618192E-5	2.973026326742729E-5	1.9820175511618192E-5	0.2873132642164173	1.9820175511618192E-5
46-47	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	0.3775347031453033	1.9820175511618192E-5
48-49	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	0.4826609140589262	1.9820175511618192E-5
50-51	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	0.5992828267692876	1.9820175511618192E-5
52-53	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	0.7155182460571725	1.9820175511618192E-5
54-55	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	0.8451719241664228	1.9820175511618192E-5
56-57	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	1.0048333480002634	1.9820175511618192E-5
58-59	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	1.1974458136221688	1.9820175511618192E-5
60-61	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	1.4005927025284994	1.9820175511618192E-5
62-63	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	1.6150767318274757	1.9820175511618192E-5
64-65	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	1.83982761204147	1.9820175511618192E-5
66-67	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	2.0992538893130406	1.9820175511618192E-5
68-69	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	2.398756561469103	1.9820175511618192E-5
70-71	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	2.72856428198243	1.9820175511618192E-5
72-73	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	3.0734749762356097	1.9820175511618192E-5
74-75	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	3.420446968741998	1.9820175511618192E-5
76-77	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	3.815007202651781	1.9820175511618192E-5
78-79	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	4.243707688880327	1.9820175511618192E-5
80-81	1.9820175511618192E-5	3.9640351023236384E-5	1.9820175511618192E-5	4.710958416478969	1.9820175511618192E-5
82-83	2.973026326742729E-5	3.9640351023236384E-5	1.9820175511618192E-5	5.195462606860477	1.9820175511618192E-5
84-85	3.9640351023236384E-5	3.9640351023236384E-5	1.9820175511618192E-5	5.672296389318987	1.9820175511618192E-5
86-87	3.9640351023236384E-5	3.9640351023236384E-5	1.9820175511618192E-5	6.180614520577702	1.9820175511618192E-5
88	3.9640351023236384E-5	3.9640351023236384E-5	1.9820175511618192E-5	6.594806638331744	1.9820175511618192E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5265	0.0	40.34787	94
GTACTAG	5165	0.0	34.793304	1
CAACGCA	9060	0.0	32.356815	5
GTATCAA	10490	0.0	31.51981	1
GCATCGT	5525	0.0	30.933073	8
TAGGCAT	6430	0.0	30.102022	5
ATCAACG	9790	0.0	30.093166	3
CTGTGCG	7430	0.0	29.57465	9
TAGTACT	7595	0.0	29.507652	4
CTAGGCA	7305	0.0	27.270287	4
GTACTGT	8165	0.0	26.9664	6
CTAGTAC	8580	0.0	26.943924	3
ACTGTGC	8565	0.0	26.53212	8
TATCAAC	12425	0.0	26.473457	2
TACCCCG	320	0.0	26.426031	5
AACGCAG	11915	0.0	26.241373	6
GGTATCA	6880	0.0	26.120262	1
TACTGTG	9260	0.0	25.859083	7
AGGGCTA	5890	0.0	25.603487	5
ACGCAGA	11515	0.0	25.280268	7
>>END_MODULE