##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5045364
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85760928250172	31.0	31.0	34.0	30.0	34.0
2	31.856636508287608	31.0	31.0	34.0	30.0	34.0
3	32.03856094426487	33.0	31.0	34.0	30.0	34.0
4	35.759410421131165	37.0	35.0	37.0	35.0	37.0
5	35.66347403279526	37.0	35.0	37.0	33.0	37.0
6	35.68937028131172	37.0	35.0	37.0	33.0	37.0
7	35.61347466704087	37.0	35.0	37.0	33.0	37.0
8	35.59800739847511	37.0	35.0	37.0	33.0	37.0
9	37.20762228453685	39.0	37.0	39.0	33.0	39.0
10-11	37.151907077467555	39.0	37.0	39.0	33.0	39.0
12-13	37.080443155340234	39.0	37.0	39.0	33.0	39.0
14-15	38.2845251601272	40.0	38.0	41.0	33.0	41.0
16-17	38.28832399406663	40.0	38.0	41.0	33.0	41.0
18-19	38.3371161129306	40.0	38.0	41.0	33.0	41.0
20-21	38.350714338945615	40.0	38.0	41.0	33.0	41.0
22-23	38.31796040880301	40.0	38.0	41.0	33.0	41.0
24-25	38.24913197541347	40.0	37.5	41.0	33.0	41.0
26-27	38.11215781457988	40.0	37.0	41.0	33.0	41.0
28-29	38.05072250485793	40.0	37.0	41.0	33.0	41.0
30-31	37.934069771774645	40.0	37.0	41.0	33.0	41.0
32-33	37.82382014459215	40.0	37.0	41.0	33.0	41.0
34-35	37.732110904188474	40.0	36.5	41.0	32.5	41.0
36-37	37.65407917050187	40.0	36.0	41.0	32.0	41.0
38-39	37.54691276982196	40.0	36.0	41.0	32.0	41.0
40-41	37.42730058723216	40.0	36.0	41.0	31.5	41.0
42-43	37.307960535652136	39.5	36.0	41.0	31.0	41.0
44-45	37.11970553561646	39.0	35.5	41.0	31.0	41.0
46-47	36.89741661850364	39.0	35.0	41.0	30.5	41.0
48-49	36.654095125743154	39.0	35.0	41.0	30.0	41.0
50-51	36.45607849106625	39.0	35.0	41.0	30.0	41.0
52-53	36.266820788351446	38.5	35.0	41.0	30.0	41.0
54-55	36.060099429892475	38.0	35.0	40.0	29.0	41.0
56-57	35.80267905348356	38.0	34.5	40.0	29.0	41.0
58-59	35.525396185488304	37.0	34.0	40.0	28.0	41.0
60-61	35.26970650680506	37.0	34.0	40.0	28.0	41.0
62-63	34.99274631126714	36.0	34.0	39.5	28.0	41.0
64-65	34.71488756807239	36.0	34.0	39.0	28.0	41.0
66-67	34.485969198654445	35.0	33.0	39.0	28.0	41.0
68-69	34.29236562515608	35.0	33.0	39.0	27.5	41.0
70-71	34.08156408536629	35.0	33.0	38.0	27.0	40.0
72-73	33.87037337643032	35.0	33.0	37.0	27.0	40.0
74-75	33.68428363146842	35.0	33.0	37.0	27.0	39.5
76-77	33.519335572220356	35.0	33.0	36.0	27.0	39.0
78-79	33.397493818087256	35.0	33.0	36.0	27.0	39.0
80-81	33.24792413391779	35.0	33.0	36.0	27.0	38.0
82-83	33.053986094957665	35.0	33.0	35.0	27.0	37.0
84-85	32.9105257816879	35.0	33.0	35.0	27.0	37.0
86-87	32.777544593412884	35.0	33.0	35.0	27.0	36.5
88-89	32.6666784596711	35.0	33.0	35.0	27.0	36.0
90-91	32.57581732457757	35.0	33.0	35.0	27.0	36.0
92-93	32.498329654708755	35.0	33.0	35.0	27.0	36.0
94-95	32.45483200022832	35.0	33.0	35.0	27.0	35.0
96-97	32.40112854097346	35.0	33.0	35.0	27.0	35.0
98-99	32.365177021915564	35.0	33.0	35.0	26.5	35.0
100	31.865997180778237	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.462394458575897
1101	2	0.5616920222424113
1101	3	0.4473652015565861
1101	4	0.3289231079792003
1101	5	0.3755469534430702
1101	6	0.3785899913580977
1101	7	0.38796929370193567
1101	8	0.4250663947456701
1101	9	0.46829178969607455
1101	10-11	0.49293258914430993
1101	12-13	0.4609822599609785
1101	14-15	0.6621116556167834
1101	16-17	0.6186619487467695
1101	18-19	0.6233659471642881
1101	20-21	0.6191096601441473
1101	22-23	0.6409880767082754
1101	24-25	0.6467989176358628
1101	26-27	0.6996817060932017
1101	28-29	0.6776571281295887
1101	30-31	0.7464654470922056
1101	32-33	0.7338184366961755
1101	34-35	0.7338899279290985
1101	36-37	0.7408001695688355
1101	38-39	0.7412407258792086
1101	40-41	0.7545679510748755
1101	42-43	0.7649763033252768
1101	44-45	0.7430868785181417
1101	46-47	0.7463268061628696
1101	48-49	0.7884924933568058
1101	50-51	0.7841280400515842
1101	52-53	0.7459406513571878
1101	54-55	0.7384627310583056
1101	56-57	0.7440701247433168
1101	58-59	0.7045104012907188
1101	60-61	0.7642717044015797
1101	62-63	0.6968358237000487
1101	64-65	0.7156946964052793
1101	66-67	0.6794833470155694
1101	68-69	0.7316509803454352
1101	70-71	0.6578103798382813
1101	72-73	0.6749947310769997
1101	74-75	0.5889854409530741
1101	76-77	0.6093222705206003
1101	78-79	0.5538344660830177
1101	80-81	0.5312244342801904
1101	82-83	0.5662747496834228
1101	84-85	0.5472658061079088
1101	86-87	0.5327267802176294
1101	88-89	0.5261217073813853
1101	90-91	0.5144553388322848
1101	92-93	0.5332800332137921
1101	94-95	0.48104538228849236
1101	96-97	0.536965797705939
1101	98-99	0.5498008826933329
1101	100	0.5459491585050316
1102	1	0.4114938094177525
1102	2	0.5422889158485376
1102	3	0.4276781411768127
1102	4	0.29140822982240877
1102	5	0.30308290132963833
1102	6	0.3066763762383502
1102	7	0.34988058835200064
1102	8	0.37613674511298
1102	9	0.39084473131298836
1102	10-11	0.40458596763686927
1102	12-13	0.4613572788906808
1102	14-15	0.5334242252435217
1102	16-17	0.5046418513882287
1102	18-19	0.5799939235966818
1102	20-21	0.5765352727329613
1102	22-23	0.5515581786706534
1102	24-25	0.5678755278748682
1102	26-27	0.6007078730988908
1102	28-29	0.6123153411588262
1102	30-31	0.6415807993938074
1102	32-33	0.6389793568838229
1102	34-35	0.5833081156894906
1102	36-37	0.5874152012880955
1102	38-39	0.5875679923760515
1102	40-41	0.5977039562311788
1102	42-43	0.6310716274102361
1102	44-45	0.546106086138451
1102	46-47	0.6427563352188201
1102	48-49	0.6266602818648863
1102	50-51	0.5692021416200248
1102	52-53	0.5738207066841952
1102	54-55	0.6364050933571477
1102	56-57	0.6869015007986974
1102	58-59	0.6637574982583558
1102	60-61	0.6268668293241362
1102	62-63	0.605156343296386
1102	64-65	0.5913682987354605
1102	66-67	0.575071545605951
1102	68-69	0.5711132991894345
1102	70-71	0.5610956910291236
1102	72-73	0.5605024957125764
1102	74-75	0.5215927112409133
1102	76-77	0.5576538174070791
1102	78-79	0.5620669646656111
1102	80-81	0.4942150255851061
1102	82-83	0.4951051604504002
1102	84-85	0.5205958521091958
1102	86-87	0.5877895610644472
1102	88-89	0.5891835485728905
1102	90-91	0.541180210051273
1102	92-93	0.50656737144282
1102	94-95	0.5112340604201293
1102	96-97	0.5312736822099566
1102	98-99	0.5144835618374657
1102	100	0.5655508370680451
1103	1	0.26212475187106676
1103	2	-0.09297212734010074
1103	3	0.21218961954777882
1103	4	0.1959022021968977
1103	5	0.1908172305397784
1103	6	0.2659129733333785
1103	7	0.3135481578225452
1103	8	0.2900296638505324
1103	9	0.3620975680836125
1103	10-11	0.3929719645836016
1103	12-13	0.40218710156766946
1103	14-15	0.4544296479176637
1103	16-17	0.4884416085389631
1103	18-19	0.5128199700662677
1103	20-21	0.524304098538984
1103	22-23	0.4823085606682227
1103	24-25	0.4599496787326629
1103	26-27	0.4925564242844871
1103	28-29	0.5711108442577668
1103	30-31	0.5506703308510339
1103	32-33	0.5842399412394101
1103	34-35	0.5676803525716281
1103	36-37	0.5693372599279343
1103	38-39	0.5727278629569241
1103	40-41	0.6340985018325611
1103	42-43	0.6671807759335877
1103	44-45	0.6330396179547648
1103	46-47	0.6330377292175058
1103	48-49	0.5615716443460599
1103	50-51	0.6110249610394263
1103	52-53	0.6084467190889598
1103	54-55	0.5887571686836921
1103	56-57	0.5779168024029957
1103	58-59	0.5347829298462798
1103	60-61	0.6021690258346339
1103	62-63	0.5798064814706123
1103	64-65	0.5586443851653513
1103	66-67	0.6148720230808209
1103	68-69	0.5615656089065695
1103	70-71	0.4734355627902289
1103	72-73	0.46011279717756537
1103	74-75	0.5023311556373642
1103	76-77	0.5033017842523293
1103	78-79	0.4539413100161056
1103	80-81	0.43717971856785454
1103	82-83	0.39702649105135634
1103	84-85	0.4409296054263194
1103	86-87	0.4440887773318849
1103	88-89	0.4633788099311502
1103	90-91	0.47099974927623833
1103	92-93	0.43457734019468575
1103	94-95	0.4570654470354967
1103	96-97	0.41895578289582147
1103	98-99	0.4426037331807464
1103	100	0.45536596040977173
1104	1	0.3165280992027313
1104	2	0.4179183209402382
1104	3	0.3408348585486678
1104	4	0.25381701961540415
1104	5	0.2670556781928326
1104	6	0.27639191256562157
1104	7	0.28802530889213074
1104	8	0.29369381561669883
1104	9	0.344916564032971
1104	10-11	0.3540348799586255
1104	12-13	0.38628316635218596
1104	14-15	0.4320852103047059
1104	16-17	0.40352122309969474
1104	18-19	0.388771760192391
1104	20-21	0.4415734872835415
1104	22-23	0.4323831353539802
1104	24-25	0.42198993010207886
1104	26-27	0.49480277635053227
1104	28-29	0.49208968557353927
1104	30-31	0.5009758048950772
1104	32-33	0.5156080585969818
1104	34-35	0.4920711834705287
1104	36-37	0.48503421024694404
1104	38-39	0.5018586755763721
1104	40-41	0.5233943627377826
1104	42-43	0.538671262892862
1104	44-45	0.5254366189388122
1104	46-47	0.4969624684106293
1104	48-49	0.4432431022465977
1104	50-51	0.48466105229347534
1104	52-53	0.4032459267996984
1104	54-55	0.3896693943956606
1104	56-57	0.43355559284069756
1104	58-59	0.4223013998699727
1104	60-61	0.4534723896696491
1104	62-63	0.3763299911767959
1104	64-65	0.35493130082988245
1104	66-67	0.3412894329758487
1104	68-69	0.36765616258507805
1104	70-71	0.39341277346950676
1104	72-73	0.4126300621741805
1104	74-75	0.4267530987570112
1104	76-77	0.34408487026636436
1104	78-79	0.2842710106868651
1104	80-81	0.2983424160241839
1104	82-83	0.30121744594355704
1104	84-85	0.2796774035890266
1104	86-87	0.3187222482616008
1104	88-89	0.3218298355259819
1104	90-91	0.346513987203771
1104	92-93	0.28452865787061654
1104	94-95	0.27023077854756394
1104	96-97	0.34979821681439915
1104	98-99	0.3280081362265008
1104	100	0.41784422115658515
1105	1	0.08493090566944517
1105	2	-0.4472752261142112
1105	3	0.038179180892988995
1105	4	0.10303914773790979
1105	5	0.15614911921296937
1105	6	0.21805840709865976
1105	7	0.2649981324345063
1105	8	0.23494891077216806
1105	9	0.34261341605313334
1105	10-11	0.3077214659290419
1105	12-13	0.3190865002363452
1105	14-15	0.3498057651258222
1105	16-17	0.3708115299106751
1105	18-19	0.3992585713136023
1105	20-21	0.3733388553740937
1105	22-23	0.38393741597310793
1105	24-25	0.37929992900042464
1105	26-27	0.31299609618859137
1105	28-29	0.37949363500755595
1105	30-31	0.3612975398643741
1105	32-33	0.35570495261774937
1105	34-35	0.3447097338299372
1105	36-37	0.3508345283498926
1105	38-39	0.3850948394751299
1105	40-41	0.39068837042183446
1105	42-43	0.3618788353955438
1105	44-45	0.3985078616059141
1105	46-47	0.3580773090837397
1105	48-49	0.3473345465166062
1105	50-51	0.30487651195912235
1105	52-53	0.32908817665510526
1105	54-55	0.30762405095464374
1105	56-57	0.33777484633156973
1105	58-59	0.3365969257726462
1105	60-61	0.3271202799998676
1105	62-63	0.3687612754313889
1105	64-65	0.32688642903501375
1105	66-67	0.2807031286160466
1105	68-69	0.3154321100085795
1105	70-71	0.3031446064861143
1105	72-73	0.27061207076709337
1105	74-75	0.2660878711274961
1105	76-77	0.2500233083946526
1105	78-79	0.2171965594488796
1105	80-81	0.2558835043218437
1105	82-83	0.25392502188891797
1105	84-85	0.16101063019951312
1105	86-87	0.17102556837507166
1105	88-89	0.17667298736160575
1105	90-91	0.11966165720294697
1105	92-93	0.15612213331748137
1105	94-95	0.12726519150302806
1105	96-97	0.1739731803220721
1105	98-99	0.18557056909438074
1105	100	0.15508395510684636
1106	1	0.2899836948707808
1106	2	0.35346635247307745
1106	3	0.274729536628314
1106	4	0.1937642156414583
1106	5	0.2109755243105198
1106	6	0.1984833244819484
1106	7	0.2542468593221301
1106	8	0.21945232537553494
1106	9	0.20165939338025396
1106	10-11	0.3029997911092792
1106	12-13	0.31287094252947867
1106	14-15	0.3937583279722645
1106	16-17	0.3404183271398864
1106	18-19	0.3760904236904068
1106	20-21	0.3527330721945461
1106	22-23	0.2890560979539316
1106	24-25	0.3039925981438856
1106	26-27	0.33204794125457937
1106	28-29	0.319241032062628
1106	30-31	0.2955152795209983
1106	32-33	0.2971411746975363
1106	34-35	0.3448355163006056
1106	36-37	0.3385080166281398
1106	38-39	0.30942225988820127
1106	40-41	0.29744154290550995
1106	42-43	0.37840388176002904
1106	44-45	0.40595879938275914
1106	46-47	0.3711576392662579
1106	48-49	0.3648746162790246
1106	50-51	0.37307624468779466
1106	52-53	0.35778573824250515
1106	54-55	0.32055135837527615
1106	56-57	0.3213299385181685
1106	58-59	0.41305012592992085
1106	60-61	0.3654162588362837
1106	62-63	0.33168806677143436
1106	64-65	0.36560681112159443
1106	66-67	0.3482904294892748
1106	68-69	0.35165962812641993
1106	70-71	0.34755334075077826
1106	72-73	0.4181327806914368
1106	74-75	0.3574381755274061
1106	76-77	0.3714805612451286
1106	78-79	0.3383638821221382
1106	80-81	0.3134986190173521
1106	82-83	0.34844985481026214
1106	84-85	0.36894168845488196
1106	86-87	0.38821637816782584
1106	88-89	0.2949043227230419
1106	90-91	0.2646265023777943
1106	92-93	0.24835863571667005
1106	94-95	0.3237549379555631
1106	96-97	0.35407094156449403
1106	98-99	0.3427557643198753
1106	100	0.4288147522838699
1107	1	0.056178004413048654
1107	2	-0.5228458201302963
1107	3	-0.08250504731572761
1107	4	0.04653693180941332
1107	5	0.08905420124030172
1107	6	0.10966534784056847
1107	7	0.1486914755159674
1107	8	0.1464092754509565
1107	9	0.17700485466158966
1107	10-11	0.20885548964606926
1107	12-13	0.1510801648848883
1107	14-15	0.15285052916473774
1107	16-17	0.2434606443547267
1107	18-19	0.23265731542662849
1107	20-21	0.17368616739295106
1107	22-23	0.16553157977055122
1107	24-25	0.19756635811470602
1107	26-27	0.23542228976349833
1107	28-29	0.2185076295361199
1107	30-31	0.21275810491099634
1107	32-33	0.2133144221881622
1107	34-35	0.2107057623530011
1107	36-37	0.25320186270743505
1107	38-39	0.2848240749599924
1107	40-41	0.25005548530669586
1107	42-43	0.24504632939499515
1107	44-45	0.22626863918223705
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2216	74-75	-0.8449700017620998
2216	76-77	-0.7637591040512106
2216	78-79	-0.7009605098913596
2216	80-81	-0.6274453345284599
2216	82-83	-0.5914551311161276
2216	84-85	-0.6453349377586761
2216	86-87	-0.5349722389592841
2216	88-89	-0.5846149870069937
2216	90-91	-0.6408349671192752
2216	92-93	-0.5883492512722235
2216	94-95	-0.5173785083553994
2216	96-97	-0.5854407551435798
2216	98-99	-0.5489451343082621
2216	100	-0.6108265147913521
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	11.0
13	46.0
14	218.0
15	752.0
16	1879.0
17	3918.0
18	6771.0
19	10776.0
20	15588.0
21	22570.0
22	31323.0
23	37229.0
24	39991.0
25	42854.0
26	49026.0
27	60277.0
28	76770.0
29	95633.0
30	119434.0
31	146785.0
32	182117.0
33	231598.0
34	314894.0
35	437045.0
36	653850.0
37	1117205.0
38	985196.0
39	360801.0
40	803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.4675326322934	21.31634889396705	13.607995407453691	24.60812306628586
2	14.927068570805623	20.275809440750027	40.024478955036024	24.772643033408322
3	18.104548995201462	26.30350287099453	29.703684030055754	25.88826410374825
4	12.360920536981888	17.115787190603275	38.78170147358162	31.74159079883322
5	12.771578948620258	38.6319481140359	35.11275429525543	13.48371864208841
6	29.891678776793906	38.78059144989341	17.649806832569464	13.677922940743226
7	26.255324209420554	32.1870831557771	23.767449235434608	17.790143399367736
8	26.16395962709529	35.13827743647436	19.634163164441652	19.063599771988702
9	25.9985008019243	14.590939325685918	21.400993070073834	38.009566802315945
10-11	25.63143115144913	25.865081290467845	27.419111881719534	21.08437567636349
12-13	26.083331153114024	23.579032157045557	27.82045061565429	22.517186074186125
14-15	23.268086297389036	24.623265836502124	25.494501530108554	26.61414633600028
16-17	21.990186149593384	27.196285828368318	26.797343582777867	24.016184439260435
18-19	21.428800776316635	27.228828286720248	29.201926758901838	22.140444178061287
20-21	22.93120208286281	26.460511715360052	28.474202106547924	22.134084095229216
22-23	23.366939995389796	25.952598928632803	28.240289186294888	22.440171889682517
24-25	22.822290207633205	26.082154437435477	28.93324732697654	22.162308027954776
26-27	22.770213668831396	26.387848465355606	27.8824609846543	22.959476881158697
28-29	21.94964525849869	26.921238982955444	28.510599037056593	22.618516721489275
30-31	22.788999961152456	26.447071014103248	28.40674131737571	22.35718770736859
32-33	23.239423359741735	26.66564790964537	27.561936066456255	22.53299266415664
34-35	23.06339116888059	26.50027550175013	28.32603947404932	22.11029385531996
36-37	22.84002700300712	26.062460508300294	28.00631431151449	23.0911981771781
38-39	23.01252199048473	26.045613359115414	28.68296519339338	22.25889945700647
40-41	22.55752483032373	25.451441061588874	29.157849129385255	22.833184978702143
42-43	22.692919606598345	25.61043119702648	28.313308532826515	23.383340663548665
44-45	22.886168371598163	26.435614952657527	28.04289244542118	22.635324230323125
46-47	22.968204078040753	26.310757757022092	27.326858874800706	23.39417929013645
48-49	23.793673725392882	26.11996934200061	27.318729227114375	22.76762770549214
50-51	23.19649285958357	26.155278390221206	27.150905266696316	23.49732348349891
52-53	22.610112967072347	26.62460032616081	27.0742804681684	23.691006238598444
54-55	22.699109505979322	26.566575073996418	26.841353084739193	23.892962335285066
56-57	23.76546445951616	25.949590504858026	26.72258802594622	23.562357009679587
58-59	22.540216710307483	26.45763339056752	27.59731578741864	23.404834111706357
60-61	22.709959786806053	27.028621749215464	26.830538718850335	23.43087974512815
62-63	23.135198966813892	26.310589285530238	26.414417274947855	24.139794472708015
64-65	22.34569200557185	26.428697711403977	26.88561221747331	24.33999806555087
66-67	22.98731072723395	26.106580218989155	26.87700035121351	24.029108702563384
68-69	22.47331269094572	26.064033685332156	27.24320464148486	24.219448982237264
70-71	22.454035396151582	25.729425621454325	27.304191159237096	24.512347823156993
72-73	23.014335536544042	25.78885289545016	27.56324419804002	23.633567369965775
74-75	23.12027294024096	26.203604960484927	27.118367424601665	23.55775467467245
76-77	22.832555747673958	26.117592120289135	26.67769759061589	24.37215454142102
78-79	22.601863424700923	26.279895443988444	26.79806728355348	24.320173847757157
80-81	22.288463552947526	26.68092579692513	27.320000035677555	23.710610614449788
82-83	23.117281561749635	25.582910469807874	28.294284670308166	23.00552329813432
84-85	23.354629459495836	26.09844820139507	26.920769253182677	23.626153085926422
86-87	21.94213217388891	26.741183501504974	27.369892040448406	23.946792284157706
88-89	22.864997392440397	25.581646348316443	27.23734263214262	24.316013627100542
90-91	22.53252057418013	25.631021140181925	27.36869917901494	24.467759106623006
92-93	22.85948724088475	25.918467691077506	27.073647984140763	24.148397083896985
94-95	21.73343547520747	25.813930671698444	27.773835691647818	24.678798161446267
96-97	22.029792288454285	25.666431620003376	27.428419371478945	24.875356720063387
98-99	21.387548466302416	26.375876119714793	27.309029587783222	24.92754582619957
100	22.126817219001794	25.659173963049998	26.530911419413535	25.68309739853468
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	167.0
1	117.0
2	91.0
3	125.0
4	91.0
5	63.5
6	147.0
7	404.5
8	692.0
9	783.5
10	652.5
11	528.5
12	562.0
13	654.0
14	822.5
15	1087.5
16	1395.0
17	1825.5
18	2451.0
19	3229.0
20	4146.0
21	5120.0
22	6234.0
23	7406.0
24	8480.5
25	10025.5
26	12308.0
27	14844.0
28	18910.5
29	26990.5
30	38740.0
31	50317.0
32	65450.5
33	81030.5
34	93086.0
35	106653.0
36	120444.0
37	144820.5
38	156802.5
39	148850.5
40	140122.5
41	131077.5
42	130662.5
43	139601.0
44	153087.5
45	182032.5
46	211547.5
47	230783.0
48	242887.5
49	236849.5
50	224527.0
51	228289.0
52	230571.0
53	221267.0
54	213390.0
55	186964.5
56	162718.0
57	136083.5
58	107747.5
59	103466.5
60	86488.5
61	59750.5
62	44116.0
63	36024.0
64	26253.5
65	13228.5
66	7882.5
67	5674.0
68	4128.5
69	3082.0
70	2335.5
71	1770.5
72	1290.0
73	1002.5
74	796.5
75	556.5
76	382.5
77	225.0
78	115.0
79	53.5
80	31.0
81	16.5
82	5.5
83	5.5
84	3.0
85	1.5
86	2.5
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013239877241760952
2	0.004241517559486293
3	0.0014666929878597462
4	0.027649144838707375
5	0.0028937456246962557
6	0.0
7	2.774824571626547E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009127190823100177
16-17	0.002923475887963683
18-19	0.0
20-21	9.910087755809094E-6
22-23	6.937061429066366E-4
24-25	9.910087755809094E-6
26-27	1.8829166736037282E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.756842122788366E-4
36-37	0.0
38-39	0.0
40-41	4.16223685743982E-4
42-43	7.333464939298731E-4
44-45	0.0
46-47	0.0
48-49	4.955043877904548E-4
50-51	0.0
52-53	0.0
54-55	3.6667324696493654E-4
56-57	9.910087755809094E-5
58-59	2.180219306278001E-4
60-61	9.910087755809094E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0016252543919526917
70-71	8.22537283732155E-4
72-73	0.0
74-75	2.08111842871991E-4
76-77	0.003042396941033392
78-79	5.946052653485458E-4
80-81	0.003478440802288993
82-83	0.0019721074634060096
84-85	0.00624335528615973
86-87	0.008304653539368021
88-89	0.008265013188344786
90-91	0.006481197392299149
92-93	0.00952359433333254
94-95	0.00186309649809211
96-97	0.005282076773846248
98-99	0.002100938604231528
100	0.08486999154074909
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5045364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.101378682800135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.14072393333129	15.699812551889591
2	16.41121177386456	7.910656592110758
3	6.883987882556935	4.977407964159365
4	3.5432345500887155	3.415873506146764
5	2.041983153515426	2.4607304623386836
6	1.3267188461692125	1.9185451990279108
7	0.8776766945640394	1.4807252863729357
8	0.642500399197951	1.2388116339936057
9	0.4375429687669714	0.9490849902224433
>10	2.1227039991878445	9.132062451146972
>50	0.2094975643120831	3.5958260114808507
>100	0.26236822614613475	14.281107409704196
>500	0.053499880991366495	9.070491670061015
>1k	0.043720332850414365	18.789813593550246
>5k	0.0021367079964488226	3.3686764502098185
>10k+	4.930864607189591E-4	1.7103742275849667
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	21194	0.4200687997932359	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	15681	0.31080017219768485	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13840	0.27431122908079575	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	12258	0.24295571142141578	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11961	0.2370691192944652	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11419	0.2263265841671681	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9358	0.18547720243772303	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8537	0.1692048383426845	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8242	0.16335788656675712	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7800	0.1545973689906219	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7707	0.1527540926680414	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7250	0.1436962724592319	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7085	0.14042594349981488	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7057	0.13987097858548955	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7054	0.13981151805895473	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7051	0.13975205753241987	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6808	0.13493575488309664	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6622	0.13124920223793565	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6366	0.1261752373069614	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6235	0.12357879431493943	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6133	0.12155713641275435	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5998	0.1188814127186859	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5969	0.11830662762884898	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5758	0.11412457059589755	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	5698	0.11293536006520045	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5661	0.11220201357127056	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5474	0.10849564075059796	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5383	0.10669200477904071	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5344	0.10591901793408762	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5341	0.10585955740755276	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5106	0.10120181616232247	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.9820175511618192E-5	0.0	0.0	0.0	1.9820175511618192E-5
7	1.9820175511618192E-5	0.0	0.0	0.0	1.9820175511618192E-5
8	1.9820175511618192E-5	0.0	0.0	0.0	1.9820175511618192E-5
9	1.9820175511618192E-5	0.0	0.0	1.9820175511618192E-5	1.9820175511618192E-5
10-11	1.9820175511618192E-5	0.0	0.0	2.973026326742729E-5	1.9820175511618192E-5
12-13	1.9820175511618192E-5	0.0	0.0	5.946052653485457E-5	1.9820175511618192E-5
14-15	1.9820175511618192E-5	0.0	0.0	5.946052653485457E-5	1.9820175511618192E-5
16-17	1.9820175511618192E-5	0.0	0.0	5.946052653485457E-5	1.9820175511618192E-5
18-19	1.9820175511618192E-5	0.0	0.0	5.946052653485457E-5	1.9820175511618192E-5
20-21	1.9820175511618192E-5	0.0	0.0	6.937061429066367E-5	1.9820175511618192E-5
22-23	1.9820175511618192E-5	0.0	0.0	1.1892105306970914E-4	1.9820175511618192E-5
24-25	1.9820175511618192E-5	0.0	0.0	2.08111842871991E-4	1.9820175511618192E-5
26-27	1.9820175511618192E-5	0.0	0.0	4.5586403676721834E-4	1.9820175511618192E-5
28-29	1.9820175511618192E-5	0.0	0.0	0.0014072324613248915	1.9820175511618192E-5
30-31	1.9820175511618192E-5	0.0	0.0	0.005708210547346039	1.9820175511618192E-5
32-33	1.9820175511618192E-5	0.0	0.0	0.015628208390910944	1.9820175511618192E-5
34-35	1.9820175511618192E-5	0.0	0.0	0.031286147045089314	1.9820175511618192E-5
36-37	1.9820175511618192E-5	0.0	0.0	0.061303802857435065	1.9820175511618192E-5
38-39	1.9820175511618192E-5	0.0	0.0	0.10801995653831914	1.9820175511618192E-5
40-41	1.9820175511618192E-5	0.0	0.0	0.162376787878932	1.9820175511618192E-5
42-43	1.9820175511618192E-5	0.0	0.0	0.2199643078279387	1.9820175511618192E-5
44-45	1.9820175511618192E-5	0.0	0.0	0.2905935032635901	1.9820175511618192E-5
46-47	1.9820175511618192E-5	0.0	0.0	0.38215280403951035	1.9820175511618192E-5
48-49	1.9820175511618192E-5	0.0	0.0	0.48889435925733005	1.9820175511618192E-5
50-51	1.9820175511618192E-5	0.0	0.0	0.606893774165749	1.9820175511618192E-5
52-53	1.9820175511618192E-5	0.0	0.0	0.7244274149496448	1.9820175511618192E-5
54-55	1.9820175511618192E-5	0.0	0.0	0.8551712027120343	1.9820175511618192E-5
56-57	1.9820175511618192E-5	0.0	0.0	1.0158236353214556	1.9820175511618192E-5
58-59	1.9820175511618192E-5	0.0	0.0	1.2101604562128718	1.9820175511618192E-5
60-61	1.9820175511618192E-5	0.0	0.0	1.4153190929336317	1.9820175511618192E-5
62-63	1.9820175511618192E-5	0.0	0.0	1.6310319731143283	1.9820175511618192E-5
64-65	1.9820175511618192E-5	0.0	0.0	1.8579333423713333	1.9820175511618192E-5
66-67	1.9820175511618192E-5	0.0	0.0	2.119916422283903	2.973026326742729E-5
68-69	1.9820175511618192E-5	0.0	0.0	2.4212227304115226	3.9640351023236384E-5
70-71	1.9820175511618192E-5	0.0	0.0	2.7539737469883243	3.9640351023236384E-5
72-73	1.9820175511618192E-5	0.0	0.0	3.1022241408152116	3.9640351023236384E-5
74-75	1.9820175511618192E-5	0.0	0.0	3.453090797809633	3.9640351023236384E-5
76-77	1.9820175511618192E-5	0.0	0.0	3.8546871940260408	3.9640351023236384E-5
78-79	1.9820175511618192E-5	0.0	0.0	4.291355390810256	3.9640351023236384E-5
80-81	1.9820175511618192E-5	0.0	0.0	4.767485557038104	3.9640351023236384E-5
82-83	1.9820175511618192E-5	0.0	0.0	5.260611523767166	3.9640351023236384E-5
84-85	1.9820175511618192E-5	0.0	0.0	5.74625537424059	3.9640351023236384E-5
86-87	1.9820175511618192E-5	0.0	0.0	6.263978178779569	3.9640351023236384E-5
88	1.9820175511618192E-5	0.0	0.0	6.685345200068816	3.9640351023236384E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9930	0.0	39.678505	1
GGGCACC	5470	0.0	37.755386	94
GTATCAA	15940	0.0	34.688023	1
GTACTAG	5090	0.0	33.254105	1
CAACGCA	17600	0.0	30.014359	5
CTGTGCG	7495	0.0	29.477303	9
ATCAACG	17945	0.0	29.470812	3
TAGTACT	7735	0.0	28.867403	4
TAGGCAT	6845	0.0	28.837078	5
TATCAAC	20110	0.0	27.491587	2
CTAGTAC	8610	0.0	27.353798	3
CTAGGCA	7630	0.0	26.985096	4
GTACTGT	8430	0.0	26.982113	6
GTGGTAT	3095	0.0	26.736992	1
TACCCCG	405	0.0	26.689978	5
ACTGTGC	8835	0.0	26.48994	8
AACGCAG	20925	0.0	25.985203	6
TACTGTG	9350	0.0	25.835073	7
GCATCGT	6260	0.0	25.524824	8
CTAACGC	2380	0.0	25.479788	3
>>END_MODULE