##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146439_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5725934
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.349078421092525	31.0	30.0	34.0	26.0	34.0
2	30.466047460554034	31.0	30.0	34.0	26.0	34.0
3	30.66955417229748	31.0	30.0	34.0	26.0	34.0
4	34.13524308872579	37.0	35.0	37.0	29.0	37.0
5	34.341326847288144	37.0	35.0	37.0	30.0	37.0
6	34.47823219757685	37.0	35.0	37.0	30.0	37.0
7	34.33118334930162	37.0	35.0	37.0	30.0	37.0
8	34.282950170225504	37.0	35.0	37.0	30.0	37.0
9	35.63933971296211	39.0	35.0	39.0	29.0	39.0
10-11	35.552165725277305	39.0	35.0	39.0	29.5	39.0
12-13	35.5176097034999	39.0	35.0	39.0	29.0	39.0
14-15	36.49905962939845	40.0	36.0	41.0	27.5	41.0
16-17	36.33935555317264	40.0	35.5	41.0	27.0	41.0
18-19	36.294627129827205	39.5	35.0	41.0	27.0	41.0
20-21	36.6287095694781	40.0	36.0	41.0	29.0	41.0
22-23	36.33822228827646	39.5	35.5	41.0	27.5	41.0
24-25	36.43969865876903	39.0	35.0	41.0	28.5	41.0
26-27	36.50383588074889	39.0	35.0	41.0	28.5	41.0
28-29	36.34232921650861	39.0	35.0	41.0	28.0	41.0
30-31	36.29042912475065	39.0	35.0	41.0	28.0	41.0
32-33	36.39059086255622	39.0	35.0	41.0	29.0	41.0
34-35	36.43234771829364	39.0	35.0	41.0	29.0	41.0
36-37	36.366882328717026	39.0	35.0	41.0	29.0	41.0
38-39	36.183766700768814	39.0	35.0	41.0	28.0	41.0
40-41	36.139744363103034	39.0	35.0	41.0	28.0	41.0
42-43	35.97961721179462	39.0	35.0	41.0	28.0	41.0
44-45	35.77701917276727	38.5	34.0	41.0	27.0	41.0
46-47	35.634961999212706	38.0	34.0	40.0	27.0	41.0
48-49	35.49085852194594	38.0	34.0	40.0	27.0	41.0
50-51	35.25465155553661	38.0	33.5	40.0	26.0	41.0
52-53	35.043811629683475	37.5	33.0	40.0	26.0	41.0
54-55	34.83731029732442	37.0	33.0	40.0	26.0	41.0
56-57	34.66440968407949	36.5	33.0	40.0	26.0	41.0
58-59	34.37337926004736	36.0	33.0	40.0	26.0	41.0
60-61	34.11981503803572	36.0	32.5	39.0	26.0	41.0
62-63	33.842451903916455	35.0	32.0	39.0	25.5	41.0
64-65	33.54454888931657	35.0	32.0	38.5	25.0	41.0
66-67	33.26197149670254	35.0	32.0	38.0	25.0	40.0
68-69	32.976129745819634	35.0	31.0	37.0	24.0	40.0
70-71	32.95384473519953	35.0	31.5	37.0	25.0	40.0
72-73	32.767933842758225	35.0	31.0	36.5	24.5	40.0
74-75	32.52241180216188	35.0	31.0	36.0	24.0	39.0
76-77	32.3174212276984	35.0	31.0	36.0	24.0	39.0
78-79	32.11678863221267	35.0	31.0	35.0	24.0	39.0
80-81	31.936906450545884	35.0	31.0	35.0	23.5	37.5
82-83	31.70629341868069	35.0	31.0	35.0	23.0	37.0
84-85	31.553810871728523	34.0	31.0	35.0	23.0	36.5
86-87	31.403460553335055	34.5	31.0	35.0	23.0	36.0
88-89	31.43285540839276	35.0	31.0	35.0	23.0	36.0
90-91	31.367364782758585	35.0	31.0	35.0	23.0	36.0
92-93	31.21973192495757	35.0	31.0	35.0	23.0	36.0
94-95	31.21789720244767	35.0	31.0	35.0	23.0	35.0
96-97	31.132521349355407	34.5	31.0	35.0	23.0	35.0
98-99	31.064729265129497	34.0	31.0	35.0	22.0	35.0
100	30.560376001539662	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8190228479171218
1101	2	0.9438931375344772
1101	3	0.9382766827840676
1101	4	0.905574526770657
1101	5	0.8848381138112202
1101	6	0.5955931878675713
1101	7	0.7196917440892747
1101	8	0.7329883246852518
1101	9	0.873644341890774
1101	10-11	0.8694790588032362
1101	12-13	0.7677154246229918
1101	14-15	0.953156018923039
1101	16-17	1.0855615589924597
1101	18-19	1.1763853712952823
1101	20-21	0.8392969436454365
1101	22-23	1.2166890999498392
1101	24-25	1.0570871558891781
1101	26-27	0.9606477290160527
1101	28-29	1.1510726104532978
1101	30-31	1.1447022189923146
1101	32-33	0.9994424969142699
1101	34-35	0.890526339180596
1101	36-37	0.8792806615088296
1101	38-39	0.9210630297750626
1101	40-41	0.9015667476967337
1101	42-43	0.9383816807240848
1101	44-45	0.9851517461429538
1101	46-47	0.9561910828634197
1101	48-49	0.9158261993578591
1101	50-51	0.8927424161254081
1101	52-53	0.9270383761532202
1101	54-55	0.8890227779254403
1101	56-57	0.7430444231633331
1101	58-59	0.7486701399455029
1101	60-61	0.794296622431041
1101	62-63	0.7235211483146742
1101	64-65	0.7430927019950886
1101	66-67	0.7537839388507592
1101	68-69	0.7790456032785045
1101	70-71	0.7163966757291362
1101	72-73	0.7037335079033227
1101	74-75	0.699722429668725
1101	76-77	0.6788820999773151
1101	78-79	0.660261477610085
1101	80-81	0.6774682107398498
1101	82-83	0.6275517707607747
1101	84-85	0.6452562908728936
1101	86-87	0.6790469445361431
1101	88-89	0.6935740155970542
1101	90-91	0.6856707205148744
1101	92-93	0.7383908126089374
1101	94-95	0.6535242627498228
1101	96-97	0.6687781389303957
1101	98-99	0.6826761566027706
1101	100	0.740937699784542
1102	1	0.6986279029314808
1102	2	0.816537376828709
1102	3	0.7938741361616124
1102	4	0.9420998153880973
1102	5	0.7566347978484984
1102	6	0.5278600972526561
1102	7	0.6144244624730817
1102	8	0.6459668923476656
1102	9	0.7727877196665105
1102	10-11	0.7936704687991281
1102	12-13	0.6970928710925222
1102	14-15	0.9059215561789529
1102	16-17	1.095269634486634
1102	18-19	1.141895543759233
1102	20-21	0.8343030256125914
1102	22-23	1.122446979141813
1102	24-25	0.9643981642993253
1102	26-27	0.8418924070011755
1102	28-29	1.0117339676806978
1102	30-31	1.0142561857957304
1102	32-33	0.9275000915224894
1102	34-35	0.7997498278145017
1102	36-37	0.7649424063138497
1102	38-39	0.8415906307040615
1102	40-41	0.7172936478227498
1102	42-43	0.8191614482641612
1102	44-45	0.8183309532118699
1102	46-47	0.8101364899068244
1102	48-49	0.7599053809693217
1102	50-51	0.8593093835610048
1102	52-53	0.840168921220986
1102	54-55	0.8986329541842508
1102	56-57	0.7604672637674597
1102	58-59	0.7505161447353856
1102	60-61	0.7255920300199321
1102	62-63	0.720879943870635
1102	64-65	0.6599209854835024
1102	66-67	0.6970103557444887
1102	68-69	0.7558786666298758
1102	70-71	0.7436195273263664
1102	72-73	0.6785161551581567
1102	74-75	0.6505414268496139
1102	76-77	0.6236137274019029
1102	78-79	0.6201294986878239
1102	80-81	0.6677773348180942
1102	82-83	0.7019112250159907
1102	84-85	0.6664770528395252
1102	86-87	0.69715694590219
1102	88-89	0.6642401709570542
1102	90-91	0.669902353498653
1102	92-93	0.8035235967008525
1102	94-95	0.7583236455278985
1102	96-97	0.727319154273097
1102	98-99	0.6911722494810881
1102	100	0.7268421673565086
1103	1	0.5267641960544012
1103	2	0.6825429426293681
1103	3	0.619012380874878
1103	4	0.6524054700169657
1103	5	0.6091193240626822
1103	6	0.45240849306121333
1103	7	0.49364460514173913
1103	8	0.5354743804820288
1103	9	0.5654343148531211
1103	10-11	0.7069246179128683
1103	12-13	0.5833415567910265
1103	14-15	0.7091104072342702
1103	16-17	0.9261263718091683
1103	18-19	1.0202659337120465
1103	20-21	0.6844212825658786
1103	22-23	0.9151459229178442
1103	24-25	0.7991587630007402
1103	26-27	0.7275053064950114
1103	28-29	0.946896027149208
1103	30-31	0.9095732371594565
1103	32-33	0.7088544435422506
1103	34-35	0.7384387129554852
1103	36-37	0.7178583691556923
1103	38-39	0.8059233636810959
1103	40-41	0.7076264970044193
1103	42-43	0.7418943395096349
1103	44-45	0.7790295818688975
1103	46-47	0.7503009862304282
1103	48-49	0.7483220868374971
1103	50-51	0.758301483136556
1103	52-53	0.7820397041478202
1103	54-55	0.7456030522487325
1103	56-57	0.730475015365748
1103	58-59	0.6968738927350273
1103	60-61	0.6430410657912802
1103	62-63	0.5885293016140594
1103	64-65	0.5814304660493619
1103	66-67	0.6190176744779947
1103	68-69	0.6460253957703088
1103	70-71	0.5895299517491068
1103	72-73	0.5856748530149929
1103	74-75	0.537665564362932
1103	76-77	0.5220268587707153
1103	78-79	0.5533022218568249
1103	80-81	0.5725240418786264
1103	82-83	0.5235761099983307
1103	84-85	0.48438488623827425
1103	86-87	0.5914392136060975
1103	88-89	0.5738156757831696
1103	90-91	0.5688573107482497
1103	92-93	0.6414847670961272
1103	94-95	0.621821192464779
1103	96-97	0.6198613707904563
1103	98-99	0.6620298565402472
1103	100	0.7043840830878167
1104	1	0.5331377536953745
1104	2	0.7091599078822632
1104	3	0.6792916521613392
1104	4	0.7049100748074579
1104	5	0.6491721786552134
1104	6	0.4031421558656447
1104	7	0.4415246747345236
1104	8	0.46848258903364126
1104	9	0.5415286470201863
1104	10-11	0.5884585761126004
1104	12-13	0.502462823766777
1104	14-15	0.6348308712418955
1104	16-17	0.8369985858586872
1104	18-19	0.766117103943273
1104	20-21	0.6320137022100383
1104	22-23	0.8491574514696509
1104	24-25	0.7074168676812107
1104	26-27	0.6343413594408318
1104	28-29	0.8146512444560017
1104	30-31	0.8935091171100353
1104	32-33	0.6921535416441884
1104	34-35	0.5556407261099281
1104	36-37	0.5428096127725865
1104	38-39	0.6762946285801803
1104	40-41	0.5926681484149157
1104	42-43	0.5939245377324838
1104	44-45	0.6362684529812341
1104	46-47	0.5732872113030538
1104	48-49	0.6116083151371399
1104	50-51	0.6733468554322357
1104	52-53	0.6916164299229166
1104	54-55	0.6450215626229792
1104	56-57	0.5869799165785423
1104	58-59	0.5771326636608549
1104	60-61	0.6401170546639676
1104	62-63	0.5373475719406287
1104	64-65	0.5845185968585866
1104	66-67	0.6127062784251365
1104	68-69	0.5862132866274976
1104	70-71	0.5262480454355725
1104	72-73	0.5075820390053281
1104	74-75	0.4514826820759623
1104	76-77	0.4547782653292529
1104	78-79	0.464227661530181
1104	80-81	0.5165812430248629
1104	82-83	0.4669684879883107
1104	84-85	0.45672914660296726
1104	86-87	0.47171949377551314
1104	88-89	0.42646285828873687
1104	90-91	0.46088818256660247
1104	92-93	0.4330877346014894
1104	94-95	0.45036977841010284
1104	96-97	0.4794103396984859
1104	98-99	0.5534151615760585
1104	100	0.5564802815876497
1105	1	0.5327480170588608
1105	2	0.6325273688855582
1105	3	0.6088674283643201
1105	4	0.6907161811872271
1105	5	0.5980810611930991
1105	6	0.38261351432186785
1105	7	0.5023596918431679
1105	8	0.5063674698744904
1105	9	0.5950976523765448
1105	10-11	0.6462608881867524
1105	12-13	0.5320229142667401
1105	14-15	0.7158908851337102
1105	16-17	0.8476468639218737
1105	18-19	0.8380708938265471
1105	20-21	0.6331930577982803
1105	22-23	0.9339736451236504
1105	24-25	0.7728484118973
1105	26-27	0.5578240763726328
1105	28-29	0.8016403344270628
1105	30-31	0.7892912160388832
1105	32-33	0.6701249308811441
1105	34-35	0.5602649151494035
1105	36-37	0.48945336115303206
1105	38-39	0.6785453016447605
1105	40-41	0.4465525405319326
1105	42-43	0.43104955433854997
1105	44-45	0.5270747393608772
1105	46-47	0.47996856972719826
1105	48-49	0.4875956093357914
1105	50-51	0.5577103303167519
1105	52-53	0.5588788095986033
1105	54-55	0.5198732791368812
1105	56-57	0.4997153809779462
1105	58-59	0.5552122055782434
1105	60-61	0.5727748512832562
1105	62-63	0.5173784575761431
1105	64-65	0.43365329649022755
1105	66-67	0.5877838960497002
1105	68-69	0.5818231737988953
1105	70-71	0.5566912937168027
1105	72-73	0.5016998239956649
1105	74-75	0.455134502648626
1105	76-77	0.4683822456534017
1105	78-79	0.47970509171047837
1105	80-81	0.5670271105969995
1105	82-83	0.49457785853206815
1105	84-85	0.4617685319494882
1105	86-87	0.5226303822299236
1105	88-89	0.4957881790563974
1105	90-91	0.5466664335208407
1105	92-93	0.5743929286848832
1105	94-95	0.5529647782428313
1105	96-97	0.5518541900500047
1105	98-99	0.5358789351381539
1105	100	0.6548930849911798
1106	1	0.46646263284051415
1106	2	0.5122860888312886
1106	3	0.49471208158159996
1106	4	0.655901409994641
1106	5	0.538697267217465
1106	6	0.28085147872700844
1106	7	0.2659778291967285
1106	8	0.33327339389565225
1106	9	0.45397698057713853
1106	10-11	0.4902230585405718
1106	12-13	0.39084683071335036
1106	14-15	0.5563632406763688
1106	16-17	0.6559624037707366
1106	18-19	0.5841074925501317
1106	20-21	0.47694350997134904
1106	22-23	0.8199274649349917
1106	24-25	0.594775777649069
1106	26-27	0.4641012478632618
1106	28-29	0.7246046778424216
1106	30-31	0.6608039941082424
1106	32-33	0.5890466447099101
1106	34-35	0.541829091952593
1106	36-37	0.44737932133590164
1106	38-39	0.5876160679059623
1106	40-41	0.512242068158173
1106	42-43	0.4790569142741887
1106	44-45	0.4634712283830993
1106	46-47	0.5138731766156468
1106	48-49	0.5543368093016809
1106	50-51	0.5739955425196115
1106	52-53	0.4930207447384447
1106	54-55	0.4838290525382476
1106	56-57	0.4410917183857208
1106	58-59	0.4779089510755625
1106	60-61	0.4845392789052596
1106	62-63	0.4345201652507953
1106	64-65	0.48523446791772784
1106	66-67	0.45585450506851544
1106	68-69	0.4226504251198975
1106	70-71	0.35568127948100425
1106	72-73	0.34877985096365194
1106	74-75	0.3832939161810458
1106	76-77	0.40280435101977474
1106	78-79	0.42425975676545846
1106	80-81	0.46810336850325385
1106	82-83	0.4188730453059861
1106	84-85	0.38214788731841054
1106	86-87	0.3733481193843886
1106	88-89	0.47239430031861573
1106	90-91	0.4006391716967741
1106	92-93	0.4513414720729081
1106	94-95	0.42243423997905083
1106	96-97	0.3795449094400283
1106	98-99	0.3522884740648031
1106	100	0.35628740206490406
1107	1	0.49297005745915357
1107	2	0.6261925725504689
1107	3	0.5452178328461308
1107	4	0.7296962033437637
1107	5	0.6283263518505322
1107	6	0.42571784983491057
1107	7	0.3835984886121935
1107	8	0.43038917875251315
1107	9	0.4991948321761157
1107	10-11	0.5710862381442112
1107	12-13	0.4581375527825955
1107	14-15	0.6095287138851404
1107	16-17	0.7236678052860768
1107	18-19	0.8351327780259652
1107	20-21	0.5623040743145609
1107	22-23	0.8331409396349088
1107	24-25	0.5463362527991293
1107	26-27	0.47888963757549874
1107	28-29	0.6777000452742996
1107	30-31	0.6522205274067403
1107	32-33	0.5891025991762078
1107	34-35	0.435969018883938
1107	36-37	0.46684537918719116
1107	38-39	0.49416751691421723
1107	40-41	0.3010837873760366
1107	42-43	0.4813091460064669
1107	44-45	0.44408349082219445
1107	46-47	0.46676694466087554
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2216	4	-0.7057539690856558
2216	5	-0.8353831410766333
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2216	90-91	-1.4045225505617047
2216	92-93	-1.3893820832878383
2216	94-95	-1.3432440460013204
2216	96-97	-1.3086087499785783
2216	98-99	-1.288618844169374
2216	100	-1.2430966724224248
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	23412.0
1	3844.0
2	618.0
3	629.0
4	572.0
5	295.0
6	304.0
7	357.0
8	687.0
9	1212.0
10	2073.0
11	4291.0
12	7594.0
13	11522.0
14	14733.0
15	18501.0
16	22314.0
17	26341.0
18	30403.0
19	34591.0
20	39280.0
21	44117.0
22	50228.0
23	58079.0
24	65178.0
25	73615.0
26	82527.0
27	93963.0
28	110852.0
29	130011.0
30	154168.0
31	183516.0
32	223460.0
33	282121.0
34	387803.0
35	519709.0
36	764655.0
37	1138287.0
38	827124.0
39	292779.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.778553648600905	21.525379090366577	11.993245954814153	23.702821306218368
2	15.422441506041269	19.75459509242813	40.80543823871237	24.017525162818234
3	18.37819482966027	27.591869038416483	28.59269996393766	25.43723616798559
4	11.980004579505747	16.68529759330304	40.99812170080387	30.33657612638734
5	12.884777362618163	39.04078016485785	35.06365553359899	13.010786938924996
6	29.060768167422246	39.14542027982913	18.429352944923743	13.364458607824876
7	26.50214170422226	31.731589452747354	24.495325180277362	17.270943662753023
8	26.117355477797172	35.04619427133112	20.00321214083921	18.8332381100325
9	26.236853765632056	14.593979117702203	22.160125470743505	37.00904164592224
10-11	25.932698259529772	25.796109371890395	27.323062347617004	20.948130020962825
12-13	25.97945491204895	23.740384797709417	27.448553883407527	22.83160640683411
14-15	23.449605513098955	24.736137479148073	24.84857965490988	26.96567735284309
16-17	22.07645339833849	27.207873588283338	26.35601525963788	24.35965775374029
18-19	21.33940032280883	28.392271198748652	28.07700323282881	22.1913252456137
20-21	23.37120424041793	27.340714385414174	26.721304711565725	22.566776662602173
22-23	24.082807711187957	26.95874286638945	26.34439721221211	22.61405221021048
24-25	23.333805024103633	27.056937523679636	27.325581803644848	22.283675648571883
26-27	23.269871154745076	27.19345254133368	26.056922349683315	23.479753954237932
28-29	22.083360359553023	27.912975192256262	26.848687780851428	23.154976667339287
30-31	23.125696645297683	27.45881613374115	26.734774611026268	22.680712609934897
32-33	23.75801248643099	27.69562615035417	25.50090246488822	23.045458898326622
34-35	23.66473758265559	27.294815210700428	26.77897163821616	22.261475568427823
36-37	23.157919413929704	26.872108997161696	26.39987272168211	23.570098867226484
38-39	23.47450966758428	26.919619231432407	27.13167432747965	22.474196773503667
40-41	22.87891664852184	25.87831786925892	27.89008449635816	23.35268098586108
42-43	22.933343728169415	26.28990126749399	26.934144060312526	23.84261094402407
44-45	23.02345978511331	27.424609352603074	26.85062462578955	22.70130623649407
46-47	23.009087711491542	27.0309852486903	26.328751400665855	23.6311756391523
48-49	23.872083087006725	26.4724054950693	26.68792646322393	22.967584954700044
50-51	23.396590797157472	26.31350598532241	26.61728894928125	23.672614268238874
52-53	22.720941877258472	27.07010495851837	26.36475744999714	23.84419571422602
54-55	22.67492856945323	26.967345847634437	26.326395787342626	24.031329795569704
56-57	24.04411139260774	26.281071783805437	26.056350453616684	23.618466369970136
58-59	22.605789805151836	26.781733430040187	27.260931008927475	23.351545755880498
60-61	22.691371438949666	27.622476851894522	26.292381940294955	23.393769768860853
62-63	23.299515924868423	26.71698154942488	25.661503075710286	24.321999449996415
64-65	22.261598469842276	26.97747497990562	26.33452500885768	24.426401541394423
66-67	23.207971154567	26.342288978581703	26.3456423637538	24.104097503097492
68-69	22.390811617937818	26.599308565627528	26.685759813041933	24.324120003392725
70-71	22.440980066512264	26.159500559302085	26.851741140491665	24.547778233693986
72-73	23.253204904504937	26.295014243776155	27.278027501475123	23.173753350243782
74-75	23.448189949991907	26.73326979754816	26.72227862174167	23.096261630718264
76-77	23.057439230720277	26.702722630508298	26.2411196864597	23.998718452311724
78-79	22.951339163022013	26.681775037470924	26.23970153211524	24.127184267391822
80-81	22.369438864421056	27.140841662988592	27.180923026576597	23.30879644601376
82-83	23.491282183449062	25.787586295525315	28.422234351636476	22.298897169389143
84-85	23.759782366702414	26.59770462467246	26.605912928420782	23.036600080204344
86-87	22.098289079778805	27.418120686869063	27.194565493543486	23.28902473980865
88-89	23.518099822204917	25.80492342714044	26.930008251775856	23.746968498878793
90-91	23.025110246588948	25.958950638594086	27.143978963751813	23.871960151065153
92-93	23.50918752546695	26.309957968224314	26.976665543876877	23.204188962431864
94-95	22.095669286610878	26.31778637671393	27.782375100570118	23.80416923610508
96-97	22.553491263916133	26.23885309884779	27.113035373957224	24.094620263278852
98-99	21.702147420820744	27.26043692851866	26.94989645708054	24.087519193580057
100	22.833670517832758	26.29002646036084	25.904880423394022	24.971422598412378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19671.0
1	11641.0
2	3247.0
3	2247.5
4	1195.0
5	658.0
6	492.5
7	451.0
8	463.5
9	437.0
10	398.5
11	387.0
12	370.0
13	362.0
14	366.5
15	470.5
16	568.5
17	644.5
18	813.0
19	966.0
20	1172.5
21	1479.0
22	1982.0
23	2762.5
24	3689.0
25	4798.5
26	6841.5
27	9361.0
28	14119.0
29	25933.5
30	42689.0
31	57930.0
32	79035.5
33	99638.5
34	114787.5
35	132470.5
36	148232.0
37	177008.0
38	188537.0
39	172289.5
40	155292.0
41	138477.0
42	134098.5
43	144212.5
44	162826.0
45	200390.5
46	238443.0
47	265945.0
48	281800.5
49	275470.5
50	263115.0
51	272131.0
52	278779.5
53	267296.5
54	250780.5
55	213041.0
56	184386.5
57	150131.0
58	114857.5
59	112029.5
60	93909.0
61	62635.5
62	46065.5
63	38924.5
64	28131.5
65	12331.0
66	5908.0
67	3763.5
68	2563.0
69	1857.0
70	1372.0
71	983.0
72	699.5
73	601.0
74	561.0
75	438.5
76	266.0
77	157.5
78	108.5
79	76.5
80	53.0
81	40.5
82	34.5
83	28.0
84	19.5
85	17.0
86	18.0
87	16.0
88	11.5
89	10.5
90	6.5
91	6.0
92	9.0
93	7.0
94	5.5
95	4.0
96	2.0
97	1.0
98	1.5
99	2.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9960820365725487
2	0.4463725917902651
3	0.479729595206651
4	0.46505949946331904
5	0.43276782442829415
6	0.48325740394492844
7	0.4995167600604548
8	0.50295724680026
9	0.5174003053475642
10-11	0.5304898729185492
12-13	0.5285775211520077
14-15	0.5259840577973829
16-17	0.5306732491153409
18-19	0.5292237039407021
20-21	0.528629914351091
22-23	0.49045273661903893
24-25	0.5258880035990634
26-27	0.5055070491556487
28-29	0.5118029652454953
30-31	0.5141082660051618
32-33	0.5167803191584115
34-35	0.5293808835379521
36-37	0.5471072492278115
38-39	0.536436501014507
40-41	0.541640892123451
42-43	0.5378773139892985
44-45	0.5491593161919086
46-47	0.5470024628296449
48-49	0.5193301215138002
50-51	0.5341486646545349
52-53	0.5267262947843967
54-55	0.5364452332143542
56-57	0.5237660790361887
58-59	0.5304112831199242
60-61	0.5230500386487165
62-63	0.517077213953217
64-65	0.5293808835379521
66-67	0.5273724775730911
68-69	0.5482948284070337
70-71	0.5316687198979241
72-73	0.5351004744378821
74-75	0.5318957570939518
76-77	0.5186315455260225
78-79	0.5504604139691446
80-81	0.5242812788271748
82-83	0.523146092847036
84-85	0.5323498314860073
86-87	0.840762397890021
88-89	1.10927754319208
90-91	1.0993315675660948
92-93	1.0994974793631922
94-95	1.104064419883289
96-97	1.0969913380070395
98-99	1.0929570616776232
100	1.0892196801430125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5725934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.965189620753137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.88214228116412	14.55105790610151
2	11.775892143942405	4.702158392146946
3	4.983166978110602	2.984696208895607
4	2.7173380861380956	2.17008680613766
5	1.6948105280755854	1.6918606782138912
6	1.094465119503573	1.3110722186513446
7	0.7659876780991216	1.0705162468286564
8	0.5859327436409382	0.9358606665439574
9	0.4217215600706802	0.7577775822573869
>10	2.3631172086125263	8.702438838874587
>50	0.25661444213230533	3.619599763596683
>100	0.31933127244821374	14.239220787236212
>500	0.0696523659921526	9.696400984640603
>1k	0.06492126188681498	24.69903334528036
>5k	0.0037673606762929075	5.254752098183183
>10k+	0.0011389695067862277	3.613467476411626
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	29014	0.5067120927345652	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	20584	0.3594872033104119	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	19197	0.33526408093421967	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	17553	0.3065526078365556	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	17443	0.30463152387016684	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	17426	0.3043346290753613	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	16118	0.28149119427503005	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	14741	0.2574427158957822	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	11473	0.20036905769434293	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	11436	0.19972287490564858	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	11147	0.1946756633939546	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	10246	0.17894023926926156	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	10199	0.17811941248362276	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	9746	0.17020803942204016	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	9353	0.1633445303421241	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	9137	0.15957222000812446	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	8631	0.15073523376273634	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8618	0.15050819656670858	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	8554	0.14939047498626426	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	8359	0.1459849170458479	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	8080	0.14111234953109833	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	8039	0.1403963091436262	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	8031	0.14025659394607065	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7982	0.13940083836104294	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7776	0.1358031720239877	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	7775	0.13578570762429326	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7736	0.13510459603621	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7729	0.1349823452383489	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	7578	0.13234522088448802	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	7193	0.12562142700212753	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	7188	0.12553410500365528	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	7157	0.12499270861312758	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	7126	0.12445131222259985	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6990	0.12207615386415561	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6923	0.12090603908462795	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	6913	0.12073139508768352	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6880	0.12015506989776689	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6841	0.11947395830968362	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6660	0.11631290196498947	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	6386	0.11152765644871213	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6364	0.11114343965543437	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	6327	0.11049725686673999	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	6275	0.10958910808262895	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5969	0.10424500177612944	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5955	0.10400050018040724	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	5920	0.10338924619110175	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5846	0.10209688061371298	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5783	0.10099662343296308	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	8.732199847221432E-6
16-17	0.0	0.0	0.0	3.492879938888573E-5	1.7464399694442864E-5
18-19	0.0	0.0	0.0	3.492879938888573E-5	1.7464399694442864E-5
20-21	0.0	0.0	0.0	6.985759877777146E-5	1.7464399694442864E-5
22-23	0.0	0.0	0.0	1.8337619679165005E-4	1.7464399694442864E-5
24-25	0.0	0.0	0.0	3.84216793277743E-4	1.7464399694442864E-5
26-27	0.0	0.0	0.0	6.374505888471645E-4	1.7464399694442864E-5
28-29	0.0	0.0	0.0	0.0020346025644025936	1.7464399694442864E-5
30-31	0.0	0.0	0.0	0.008068552658832603	1.7464399694442864E-5
32-33	0.0	0.0	0.0	0.021437550624928615	1.7464399694442864E-5
34-35	0.0	0.0	0.0	0.042001881265135085	1.7464399694442864E-5
36-37	0.0	0.0	0.0	0.07815318863263182	1.7464399694442864E-5
38-39	0.0	0.0	0.0	0.13537529423147385	1.7464399694442864E-5
40-41	0.0	0.0	0.0	0.197766862139871	1.7464399694442864E-5
42-43	0.0	0.0	0.0	0.267292637323448	1.7464399694442864E-5
44-45	0.0	0.0	0.0	0.35306030422285695	1.7464399694442864E-5
46-47	0.0	0.0	0.0	0.4592962475641529	1.7464399694442864E-5
48-49	0.0	0.0	0.0	0.5856773759529886	1.7464399694442864E-5
50-51	0.0	0.0	0.0	0.7204064873957681	1.7464399694442864E-5
52-53	0.0	0.0	0.0	0.8558079782267836	3.492879938888573E-5
54-55	0.0	0.0	0.0	1.0077395233685893	3.492879938888573E-5
56-57	0.0	0.0	0.0	1.1926263907338086	3.492879938888573E-5
58-59	0.0	0.0	0.0	1.4185458651811216	3.492879938888573E-5
60-61	0.0	0.0	0.0	1.6520623534955172	3.492879938888573E-5
62-63	0.0	0.0	0.0	1.9054183998627998	3.492879938888573E-5
64-65	0.0	0.0	0.0	2.169934197634831	3.492879938888573E-5
66-67	0.0	0.0	0.0	2.472950264533262	3.492879938888573E-5
68-69	0.0	0.0	0.0	2.8153834815420504	3.492879938888573E-5
70-71	0.0	0.0	0.0	3.190431464980211	3.492879938888573E-5
72-73	0.0	0.0	0.0	3.588846815209536	3.492879938888573E-5
74-75	0.0	0.0	0.0	3.9750196212530566	3.492879938888573E-5
76-77	0.0	0.0	0.0	4.4269633565458495	3.492879938888573E-5
78-79	0.0	0.0	0.0	4.905400586175111	3.492879938888573E-5
80-81	0.0	0.0	0.0	5.425010138084023	3.492879938888573E-5
82-83	0.0	0.0	0.0	5.965987383019085	4.366099923610716E-5
84-85	0.0	0.0	0.0	6.496747954132898	5.239319908332859E-5
86-87	0.0	0.0	0.0	7.064969662591291	5.239319908332859E-5
88	0.0	0.0	0.0	7.518878142849708	5.239319908332859E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	7065	0.0	45.92456	94
TATTCTC	2805	0.0	35.34452	5
GTACTAG	7045	0.0	34.4964	1
CTGTGCG	10765	0.0	31.79129	9
TAGGCAT	8500	0.0	31.619106	5
CAACGCA	11005	0.0	31.594694	5
TAGTACT	11000	0.0	31.482294	4
ACTGTGC	11500	0.0	30.206598	8
GTATCAA	13345	0.0	30.127874	1
GCATCGT	7550	0.0	29.906532	8
GTACTGT	11590	0.0	29.64749	6
CTAGGCA	9360	0.0	29.468216	4
TACTGTG	12335	0.0	29.455467	7
CAGTACG	1115	0.0	29.051521	9
ATCAACG	12010	0.0	29.031895	3
AGGGCTA	7285	0.0	28.70139	5
CTAGTAC	12765	0.0	28.345518	3
TCTAACG	3940	0.0	28.027863	2
CTAACGC	4005	0.0	27.807398	3
TACCTGG	1785	0.0	27.11548	2
>>END_MODULE