##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146438_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5548356
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.55731823985339	31.0	31.0	34.0	27.0	34.0
2	30.687960722058932	31.0	31.0	34.0	27.0	34.0
3	30.860311595002194	31.0	31.0	34.0	27.0	34.0
4	34.33333892057395	37.0	35.0	37.0	30.0	37.0
5	34.55767041624582	37.0	35.0	37.0	30.0	37.0
6	34.67052564759724	37.0	35.0	37.0	31.0	37.0
7	34.56533484873717	37.0	35.0	37.0	30.0	37.0
8	34.51577782680131	37.0	35.0	37.0	30.0	37.0
9	35.89869593804003	39.0	35.0	39.0	30.0	39.0
10-11	35.817529733131764	39.0	35.0	39.0	30.0	39.0
12-13	35.79898198673625	39.0	35.0	39.0	30.0	39.0
14-15	36.81734265068788	40.0	36.0	41.0	29.5	41.0
16-17	36.65994584702207	40.0	36.0	41.0	28.5	41.0
18-19	36.603971338537036	40.0	36.0	41.0	28.5	41.0
20-21	36.94040784333233	40.0	36.0	41.0	30.0	41.0
22-23	36.641465868448236	40.0	36.0	41.0	28.5	41.0
24-25	36.74846071521006	40.0	36.0	41.0	29.5	41.0
26-27	36.81813099231556	40.0	36.0	41.0	30.0	41.0
28-29	36.65755567955625	40.0	35.5	41.0	29.5	41.0
30-31	36.60451627473075	40.0	35.0	41.0	29.5	41.0
32-33	36.71084056250176	40.0	35.0	41.0	30.0	41.0
34-35	36.761026960058075	40.0	35.0	41.0	30.0	41.0
36-37	36.6901397458995	40.0	35.0	41.0	30.0	41.0
38-39	36.51539843514007	39.0	35.0	41.0	29.0	41.0
40-41	36.46690172728643	39.0	35.0	41.0	29.0	41.0
42-43	36.30223682474592	39.0	35.0	41.0	29.0	41.0
44-45	36.095704295110124	39.0	35.0	41.0	28.0	41.0
46-47	35.9563740142125	38.5	34.5	41.0	28.0	41.0
48-49	35.81630081054641	38.0	34.0	40.0	28.0	41.0
50-51	35.57455523762354	38.0	34.0	40.0	27.5	41.0
52-53	35.3680337022354	38.0	34.0	40.0	27.0	41.0
54-55	35.15838790805781	37.0	33.5	40.0	27.0	41.0
56-57	34.986338926341425	37.0	33.0	40.0	27.0	41.0
58-59	34.69683947821661	36.5	33.0	40.0	26.0	41.0
60-61	34.435831803150336	36.0	33.0	39.5	26.0	41.0
62-63	34.1586494269654	35.0	33.0	39.0	26.0	41.0
64-65	33.85824242352149	35.0	32.5	39.0	26.0	41.0
66-67	33.57357062164	35.0	32.0	38.0	26.0	40.0
68-69	33.28651234708083	35.0	32.0	37.5	25.0	40.0
70-71	33.25243432469006	35.0	32.0	37.0	25.5	40.0
72-73	33.06616203069882	35.0	32.0	36.5	25.0	40.0
74-75	32.81826283317076	35.0	32.0	36.0	25.0	39.0
76-77	32.60971060616875	35.0	32.0	36.0	25.0	39.0
78-79	32.40548551679092	35.0	32.0	35.5	24.5	39.0
80-81	32.21686568057277	35.0	31.5	35.0	24.0	37.5
82-83	31.99070120951143	35.0	31.0	35.0	24.0	37.0
84-85	31.833977037522466	35.0	31.0	35.0	24.0	37.0
86-87	31.68697313222151	35.0	31.0	35.0	24.0	36.0
88-89	31.70914357333956	35.0	32.0	35.0	24.0	36.0
90-91	31.643734918956174	35.0	32.0	35.0	24.0	36.0
92-93	31.499586904661488	35.0	31.0	35.0	23.5	36.0
94-95	31.50022204775613	35.0	31.0	35.0	24.0	35.0
96-97	31.418048697668283	35.0	31.0	35.0	23.5	35.0
98-99	31.346864278355607	35.0	31.0	35.0	23.0	35.0
100	30.854418678253523	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7378151676055218
1101	2	0.8935025916921511
1101	3	0.8831264190093364
1101	4	0.9284773591281663
1101	5	0.820431267730811
1101	6	0.6154008328714724
1101	7	0.6669685077963763
1101	8	0.6984962055071904
1101	9	0.9092792022210929
1101	10-11	0.8795594393227049
1101	12-13	0.7611339225684048
1101	14-15	1.0082928615494424
1101	16-17	1.1082146769290944
1101	18-19	1.2269073029812105
1101	20-21	0.8774798878979411
1101	22-23	1.216302361484047
1101	24-25	1.1152812859890702
1101	26-27	0.9332020896070503
1101	28-29	1.1659748762632418
1101	30-31	1.175956821737259
1101	32-33	0.9940968124826668
1101	34-35	0.9466183344000996
1101	36-37	0.9008374916344764
1101	38-39	1.006212213887828
1101	40-41	0.9288354502460479
1101	42-43	0.9539937203098816
1101	44-45	0.9546269879382407
1101	46-47	0.9864576665303417
1101	48-49	0.974486444037268
1101	50-51	1.0059640606848603
1101	52-53	0.9313320365676461
1101	54-55	0.9423675830896627
1101	56-57	0.8254913265311217
1101	58-59	0.8083800226464106
1101	60-61	0.8022103796696527
1101	62-63	0.7011591006613216
1101	64-65	0.758848257957105
1101	66-67	0.757814495556822
1101	68-69	0.7331768196910815
1101	70-71	0.7527071541110004
1101	72-73	0.7143564159061526
1101	74-75	0.703781070490308
1101	76-77	0.7033468796962765
1101	78-79	0.7530350155143068
1101	80-81	0.6966586310184937
1101	82-83	0.6564825898088316
1101	84-85	0.6721310479225693
1101	86-87	0.6580614003300731
1101	88-89	0.6309631132158025
1101	90-91	0.6309008020868951
1101	92-93	0.7286356938087195
1101	94-95	0.7409852457113679
1101	96-97	0.7158928507264584
1101	98-99	0.7090292291233844
1101	100	0.8128173895351409
1102	1	0.7444637875580256
1102	2	0.8796618515727417
1102	3	0.8709255809598382
1102	4	0.8976613656050887
1102	5	0.7850840596742188
1102	6	0.5860422617388252
1102	7	0.6217077971778977
1102	8	0.6980794808003736
1102	9	0.8118684040069084
1102	10-11	0.8840489224469366
1102	12-13	0.770847752627688
1102	14-15	1.0015057718070821
1102	16-17	1.220792147056919
1102	18-19	1.199490428340333
1102	20-21	0.9550788397107439
1102	22-23	1.1901765442319672
1102	24-25	1.0775860069284136
1102	26-27	0.9919969445828514
1102	28-29	1.1336249120360975
1102	30-31	1.1799610676170502
1102	32-33	1.0611693577148102
1102	34-35	0.9048762011588778
1102	36-37	0.8832371152324043
1102	38-39	0.9178542564354615
1102	40-41	0.887707751989872
1102	42-43	0.8988753909922806
1102	44-45	0.8975093604213313
1102	46-47	0.83549045522782
1102	48-49	0.782398923367424
1102	50-51	0.8746635702437686
1102	52-53	0.8192799753767233
1102	54-55	0.8941240901635155
1102	56-57	0.8049940816235264
1102	58-59	0.7232878821959972
1102	60-61	0.7322412534373868
1102	62-63	0.6495945753205277
1102	64-65	0.6737979064170574
1102	66-67	0.7305636696616133
1102	68-69	0.791054748059409
1102	70-71	0.7232872796387326
1102	72-73	0.6006558993595732
1102	74-75	0.655126947971894
1102	76-77	0.6921362318186581
1102	78-79	0.656570203516246
1102	80-81	0.7099539662001959
1102	82-83	0.7180676631607952
1102	84-85	0.6514070618786505
1102	86-87	0.6977674634308961
1102	88-89	0.7213634659274355
1102	90-91	0.7265468705726938
1102	92-93	0.7765349277649491
1102	94-95	0.7425301991218412
1102	96-97	0.7296504083450621
1102	98-99	0.7780061454583027
1102	100	0.7919107584961438
1103	1	0.6070265544481579
1103	2	0.724736367492298
1103	3	0.6786458167159957
1103	4	0.6930839745349715
1103	5	0.6383955513696478
1103	6	0.46481284007698775
1103	7	0.5688636190718057
1103	8	0.5645117400429314
1103	9	0.6277493822847475
1103	10-11	0.7440665015648307
1103	12-13	0.6695294195085211
1103	14-15	0.7996526475890349
1103	16-17	1.0412230908513749
1103	18-19	1.0559903924396181
1103	20-21	0.8104679393914012
1103	22-23	1.0148853060056382
1103	24-25	0.8984588388384083
1103	26-27	0.7806741335873681
1103	28-29	0.9826346855191517
1103	30-31	1.0070260024353175
1103	32-33	0.7760414396668551
1103	34-35	0.8166562174271519
1103	36-37	0.7436590414012585
1103	38-39	0.8733734188538804
1103	40-41	0.7747119966194802
1103	42-43	0.8098639944229546
1103	44-45	0.808929268811923
1103	46-47	0.778766769981651
1103	48-49	0.7945283564397911
1103	50-51	0.803494352420266
1103	52-53	0.8488876806046832
1103	54-55	0.8528390706023004
1103	56-57	0.7305235307828681
1103	58-59	0.6884096359694993
1103	60-61	0.6978738117422338
1103	62-63	0.6141552132049455
1103	64-65	0.5838263708608906
1103	66-67	0.6527905627334647
1103	68-69	0.6922557383722534
1103	70-71	0.5810481860806149
1103	72-73	0.5662878749073741
1103	74-75	0.6336012051473219
1103	76-77	0.6195244457086204
1103	78-79	0.5727675253375608
1103	80-81	0.6317753251234208
1103	82-83	0.5202170006236031
1103	84-85	0.5227778632760618
1103	86-87	0.613251224438347
1103	88-89	0.6034864512608102
1103	90-91	0.6741167449403527
1103	92-93	0.6837799503363513
1103	94-95	0.6446303044066148
1103	96-97	0.6224908352225036
1103	98-99	0.6917194402183959
1103	100	0.6514635205270878
1104	1	0.522269329031726
1104	2	0.6789111154673471
1104	3	0.6131469761783492
1104	4	0.7333001415484333
1104	5	0.6629103087998303
1104	6	0.364468586243639
1104	7	0.4321411885177753
1104	8	0.42123613348937283
1104	9	0.5206961150871621
1104	10-11	0.6132258295637598
1104	12-13	0.5011197233556715
1104	14-15	0.6789111769786658
1104	16-17	0.8381680508014995
1104	18-19	0.8038802970518617
1104	20-21	0.6482681223008342
1104	22-23	0.8879510437633229
1104	24-25	0.7452842258331316
1104	26-27	0.6486021901771366
1104	28-29	0.8640443201293593
1104	30-31	0.9002732787268215
1104	32-33	0.7187524886810834
1104	34-35	0.5964683153947874
1104	36-37	0.5995695710022346
1104	38-39	0.7241362659093582
1104	40-41	0.6102512125929351
1104	42-43	0.594228672045773
1104	44-45	0.6661667454830464
1104	46-47	0.6273207632764652
1104	48-49	0.6691191299192738
1104	50-51	0.7434683019701396
1104	52-53	0.7345144930230916
1104	54-55	0.7612376946582486
1104	56-57	0.6731020092354925
1104	58-59	0.6121846457602445
1104	60-61	0.6599896953749536
1104	62-63	0.5694494040756339
1104	64-65	0.5400443012413731
1104	66-67	0.6257455125672848
1104	68-69	0.6521753362236922
1104	70-71	0.5655663046432267
1104	72-73	0.5501040418315739
1104	74-75	0.5585474222859546
1104	76-77	0.5443518598723855
1104	78-79	0.49628524724860057
1104	80-81	0.5585050654709178
1104	82-83	0.5636700988495136
1104	84-85	0.5166199857977105
1104	86-87	0.4855803893314068
1104	88-89	0.45692994649035157
1104	90-91	0.4416663588871792
1104	92-93	0.49942267401193163
1104	94-95	0.48884361613138694
1104	96-97	0.4375443006063833
1104	98-99	0.4594204976192735
1104	100	0.5800434459851616
1105	1	0.4116705364579474
1105	2	0.6294576712435287
1105	3	0.5691756574605904
1105	4	0.657809145799348
1105	5	0.5666275131713903
1105	6	0.2650823395734392
1105	7	0.3428292090429679
1105	8	0.37843672683035834
1105	9	0.45947719378283836
1105	10-11	0.4797283953514011
1105	12-13	0.4517268800126786
1105	14-15	0.6000451602433046
1105	16-17	0.6974663722594414
1105	18-19	0.6825365064017674
1105	20-21	0.5607694257314648
1105	22-23	0.8074228452690235
1105	24-25	0.6544518584905603
1105	26-27	0.44969730700739774
1105	28-29	0.7256091311256938
1105	30-31	0.7389605519056914
1105	32-33	0.6293126969423284
1105	34-35	0.5262845710901232
1105	36-37	0.45148331141528786
1105	38-39	0.6026900954507752
1105	40-41	0.4488296060970569
1105	42-43	0.5273622382126106
1105	44-45	0.5328220719575043
1105	46-47	0.44131064671864806
1105	48-49	0.4734667383418838
1105	50-51	0.5392624459502002
1105	52-53	0.5298300532327289
1105	54-55	0.39039491923194447
1105	56-57	0.42950224776676293
1105	58-59	0.41717962703371114
1105	60-61	0.41180210702172104
1105	62-63	0.3994358318016893
1105	64-65	0.32378998885669574
1105	66-67	0.4393265544211644
1105	68-69	0.41285575052241086
1105	70-71	0.37308049578741986
1105	72-73	0.40707507925723974
1105	74-75	0.3632671273081982
1105	76-77	0.395295944703669
1105	78-79	0.39150857850474097
1105	80-81	0.46342153665757024
1105	82-83	0.42151943416212845
1105	84-85	0.4083325733280887
1105	86-87	0.45390182514357136
1105	88-89	0.4073963188098908
1105	90-91	0.4432460753215217
1105	92-93	0.4553963968504462
1105	94-95	0.3966047468757843
1105	96-97	0.40112329569386773
1105	98-99	0.39941355244301846
1105	100	0.43278748208127027
1106	1	0.48644046592821155
1106	2	0.5251586144073528
1106	3	0.49884892327114727
1106	4	0.6473747208428549
1106	5	0.5318583935287364
1106	6	0.30922278161702366
1106	7	0.34011182977555876
1106	8	0.41418300675270814
1106	9	0.5793345336812123
1106	10-11	0.49313569611037167
1106	12-13	0.4399110671502484
1106	14-15	0.6083178889859724
1106	16-17	0.7352604018461477
1106	18-19	0.7127890027701156
1106	20-21	0.5627227000370638
1106	22-23	0.8500976488009044
1106	24-25	0.6700543627601689
1106	26-27	0.5217081089663651
1106	28-29	0.7298840969491991
1106	30-31	0.6840386751329177
1106	32-33	0.5844437429474638
1106	34-35	0.5763788358393498
1106	36-37	0.4964956565306551
1106	38-39	0.5401797399160699
1106	40-41	0.4719083750123403
1106	42-43	0.47470892500539463
1106	44-45	0.48570444680686364
1106	46-47	0.5002248309443758
1106	48-49	0.4892799880183958
1106	50-51	0.5231182879392406
1106	52-53	0.5188009145210799
1106	54-55	0.5545610257452012
1106	56-57	0.521458927152878
1106	58-59	0.5452715074113073
1106	60-61	0.5304287671173782
1106	62-63	0.44267517757511854
1106	64-65	0.4515570957516317
1106	66-67	0.5475780727460275
1106	68-69	0.5020388111028069
1106	70-71	0.43725681346594314
1106	72-73	0.36774139209990153
1106	74-75	0.5204618138064063
1106	76-77	0.502278989005994
1106	78-79	0.5053631052033012
1106	80-81	0.4757879711510071
1106	82-83	0.47070961334463846
1106	84-85	0.3905885913595242
1106	86-87	0.4130113327235456
1106	88-89	0.4519216463722451
1106	90-91	0.4437009783181658
1106	92-93	0.3999434239882298
1106	94-95	0.4880504060163524
1106	96-97	0.4738681126273079
1106	98-99	0.44911969100425253
1106	100	0.41662362188849755
1107	1	0.5065176622969325
1107	2	0.6814244453886005
1107	3	0.6050597642950386
1107	4	0.7765712798691666
1107	5	0.6649954151793409
1107	6	0.4316021010161464
1107	7	0.4043954686930533
1107	8	0.49253969784149376
1107	9	0.6047208191429618
1107	10-11	0.6516233907170843
1107	12-13	0.5413683100384858
1107	14-15	0.7863300345939521
1107	16-17	0.7766672645623558
1107	18-19	0.91509823627716
1107	20-21	0.6397540127084085
1107	22-23	0.9181831281133626
1107	24-25	0.6247395436009526
1107	26-27	0.598057493258807
1107	28-29	0.7416564449788936
1107	30-31	0.7372196895510328
1107	32-33	0.6432865645785952
1107	34-35	0.5289241800583682
1107	36-37	0.5433629364378589
1107	38-39	0.5513899835236415
1107	40-41	0.48098587644214064
1107	42-43	0.5381601892413244
1107	44-45	0.5126301664951214
1107	46-47	0.5219265948676508
1107	48-49	0.5115573638645188
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2216	90-91	-1.4841010155158827
2216	92-93	-1.3931549671542385
2216	94-95	-1.2969740462431858
2216	96-97	-1.3197207200402303
2216	98-99	-1.3210573838723825
2216	100	-1.3295460571903277
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	22816.0
1	3853.0
2	602.0
3	592.0
4	586.0
5	327.0
6	292.0
7	377.0
8	710.0
9	1218.0
10	2038.0
11	3683.0
12	6301.0
13	8880.0
14	11821.0
15	14849.0
16	17751.0
17	21441.0
18	24698.0
19	28093.0
20	32388.0
21	37158.0
22	42975.0
23	49590.0
24	56368.0
25	63799.0
26	72351.0
27	83910.0
28	100454.0
29	117599.0
30	140318.0
31	169822.0
32	209742.0
33	266390.0
34	371100.0
35	504890.0
36	752277.0
37	1146895.0
38	854684.0
39	304561.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96175618054072	21.829791045493455	12.309997913695442	23.89845486027039
2	15.364023796333512	19.81021229139284	41.05014175691676	23.775622155356892
3	18.28972806357468	27.461368293612743	28.870609477705667	25.37829416510691
4	12.047223463103455	16.86488876704411	40.89581384113634	30.192073928716095
5	12.906445601917637	38.832690656941864	35.18058597170969	13.08027776943081
6	28.93659426097455	39.211783706265116	18.454060501868145	13.397561530892183
7	26.395570072556218	31.846099584603728	24.439126267532878	17.319204075307173
8	26.058559990666865	35.07681087194348	20.069266647790254	18.795362489599405
9	26.200401895951668	14.727325138028306	22.229564083670166	36.84270888234986
10-11	25.875985945410857	25.77847070406765	27.358828273240913	20.98671507728058
12-13	25.995849370528934	23.705601030391136	27.43198963665412	22.866559962425804
14-15	23.376767275662342	24.711341086954768	24.911772681236215	27.000118956146675
16-17	22.15761114439768	27.217839096339265	26.42202684370269	24.202522915560365
18-19	21.4508509883221	28.25641098586861	28.09892743208216	22.193810593727132
20-21	23.382088789035667	27.280613586331874	26.683732072761906	22.653565551870553
22-23	24.127292971231366	26.810789084774978	26.370231046404356	22.691686897589296
24-25	23.41779990340448	26.929683951816756	27.24481075404753	22.407705390731234
26-27	23.28589103249663	27.157401258894133	26.114910977864415	23.441796730744827
28-29	22.2234259947285	27.841754361141476	26.820590923267446	23.114228720862577
30-31	23.138689133338307	27.305497720455605	26.7660580876177	22.789755058588383
32-33	23.77486321590666	27.618406328181994	25.49913500146348	23.107595454447864
34-35	23.662426724230876	27.268325714700925	26.774740894923394	22.294506666144805
36-37	23.215737702778856	26.861391641178145	26.418768977067263	23.504101678975736
38-39	23.515276378512883	26.825967066335682	27.104523343302333	22.554233211849102
40-41	22.925179899791637	25.8725870457652	27.878204114623	23.32402893982016
42-43	22.965613426183914	26.215909315376468	26.986770658737868	23.831706599701747
44-45	23.084529550386108	27.32520263685039	26.83674463429763	22.753523178465873
46-47	23.065800055730122	26.92484362717002	26.330941771085133	23.678414546014732
48-49	23.970737460176565	26.36196888085271	26.680284566268718	22.987009092702003
50-51	23.42381988656472	26.278022444593883	26.5708071685414	23.727350500299995
52-53	22.72391726933129	27.044901232581438	26.34193444110557	23.889247056981706
54-55	22.75380776661343	26.968296715215175	26.262240948733666	24.01565456943773
56-57	24.107014485033222	26.21768816568664	26.028948323261258	23.646349026018886
58-59	22.73043228670429	26.656369380238417	27.25142907466125	23.361769258396052
60-61	22.836463588866433	27.512142178317205	26.23863808290836	23.412756149908
62-63	23.436910023966842	26.532044327424604	25.702726001813275	24.328319646795276
64-65	22.413569098423768	26.820012550154864	26.334513994981023	24.431904356440345
66-67	23.353255926042696	26.303203922549223	26.259653261724342	24.08388688968374
68-69	22.494507411426486	26.460398764875524	26.64258642133331	24.40250740236468
70-71	22.543279437592005	26.081723189906924	26.80260923328494	24.572388139216127
72-73	23.32099684704708	26.25652848267742	27.203882196734185	23.218592473541307
74-75	23.523804372259928	26.667902520825365	26.662955479984824	23.14533762692988
76-77	23.083620726204977	26.70797791982038	26.192844995559106	24.01555635841554
78-79	22.92737251353846	26.65989902180889	26.282576540631513	24.130151924021135
80-81	22.450121873172176	27.031672528996975	27.1725442175293	23.345661380301554
82-83	23.526886221089146	25.76439529218205	28.338058002447948	22.37066048428085
84-85	23.85052633753484	26.551463943373616	26.51378103539475	23.084228683696793
86-87	22.19655134524292	27.34234987921883	27.152400940947057	23.30869783459119
88-89	23.46317825483723	25.802236757271512	26.91502008251716	23.819564905374097
90-91	23.05634536397543	25.892475414896964	27.155089620222526	23.896089600905075
92-93	23.44382796988316	26.194044606677792	27.04435427656067	23.31777314687838
94-95	22.086741722067934	26.249452457151783	27.776694917918466	23.88711090286182
96-97	22.538263468650758	26.222343024628415	27.15632219119447	24.083071315526357
98-99	21.820975545131688	27.15882789967744	26.9804171900418	24.039779365149077
100	22.843437846103722	26.174470350835062	26.007739239101163	24.97435256396006
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	19080.0
1	11194.5
2	2982.0
3	2055.0
4	1038.5
5	520.0
6	402.5
7	384.5
8	383.5
9	372.0
10	332.5
11	302.0
12	306.0
13	299.0
14	306.0
15	435.0
16	522.5
17	567.5
18	716.5
19	885.0
20	1086.0
21	1297.5
22	1719.5
23	2352.0
24	3191.5
25	4484.0
26	6561.5
27	8754.0
28	13357.0
29	25020.5
30	41306.0
31	56268.5
32	76469.5
33	95671.5
34	109353.0
35	126255.5
36	141716.5
37	170585.0
38	182715.5
39	166545.0
40	150904.0
41	134989.0
42	130482.0
43	141015.0
44	158845.0
45	195511.5
46	232917.5
47	258314.5
48	273297.5
49	266396.5
50	253314.0
51	260800.0
52	266562.0
53	256845.0
54	243188.5
55	209266.5
56	181716.5
57	148209.5
58	114044.0
59	108558.0
60	89613.5
61	60725.5
62	44935.5
63	37780.0
64	27618.0
65	12511.5
66	6137.5
67	4043.5
68	2912.0
69	2216.5
70	1654.0
71	1242.0
72	890.0
73	658.5
74	524.0
75	380.0
76	263.5
77	192.0
78	130.0
79	85.0
80	67.5
81	61.5
82	45.5
83	39.0
84	33.5
85	27.0
86	23.0
87	18.0
88	14.5
89	12.5
90	10.0
91	6.5
92	9.0
93	7.5
94	5.0
95	4.5
96	3.5
97	3.5
98	2.5
99	3.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9983137347351179
2	0.44726762305807344
3	0.48052071640680594
4	0.4658497039483407
5	0.43367801200932315
6	0.48852308683869605
7	0.5061859765307056
8	0.5089615734823072
9	0.5229837450949434
10-11	0.5358614335489648
12-13	0.5348431138881499
14-15	0.5319683884739912
16-17	0.5367445780335652
18-19	0.5350143357780214
20-21	0.533293105200892
22-23	0.4959487098520715
24-25	0.5310852439893907
26-27	0.5110072244823511
28-29	0.5167566753106686
30-31	0.5196494240816559
32-33	0.5223619392843574
34-35	0.5343745066106068
36-37	0.5528935057519742
38-39	0.5421696084389682
40-41	0.5470719614963424
42-43	0.5450803805667841
44-45	0.5549121217167752
46-47	0.55319990281806
48-49	0.5253718398747305
50-51	0.5401059340820957
52-53	0.5322657738616627
54-55	0.542412923756154
56-57	0.5295893053726185
58-59	0.5361948656502935
60-61	0.5293640134122612
62-63	0.5238308428658868
64-65	0.5352756744520358
66-67	0.5316169330158339
68-69	0.55319990281806
70-71	0.5370960334917226
72-73	0.5413675690600963
74-75	0.5389974976371379
76-77	0.5262459726809167
78-79	0.5574083566375337
80-81	0.5317611198704626
82-83	0.5308058819585477
84-85	0.5396012800908954
86-87	0.8448899097318197
88-89	1.1105722127419364
90-91	1.1010468686580313
92-93	1.1005241913100026
94-95	1.105597766257248
96-97	1.0989651709443302
98-99	1.094684623697542
100	1.0900165742789396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5548356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.358620559783713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15738367486281	14.690247948232635
2	12.13860417722173	4.942504731389255
3	5.270350387093383	3.218911912438302
4	2.863363830092929	2.3317655096588377
5	1.7230636600786182	1.7539599627946365
6	1.1570079721118744	1.413305177332228
7	0.7802407813627077	1.111923820912277
8	0.5634620663342493	0.9177048326664529
9	0.41950549530159026	0.7686497881430285
>10	2.224570611332184	8.251025594280314
>50	0.24649732632974664	3.5761178469229735
>100	0.31717432529817263	14.5095639248187
>500	0.07045126155895887	9.942700215403862
>1k	0.06380491612872295	24.350355784299435
>5k	0.00354471756270683	5.06885479974677
>10k+	9.747973297443783E-4	3.152408150960299
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	23897	0.43070415813260715	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	19636	0.35390663468602235	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	19309	0.3480129970030762	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	17234	0.31061453158377006	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	17100	0.3081994017687401	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	17003	0.30645113615636776	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	15471	0.2788393534949812	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	13606	0.24522579300967712	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	10853	0.19560749166059277	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	10426	0.18791151829478858	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	10197	0.18378416958104346	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9745	0.1756376122945247	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	9482	0.17089746944860784	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	9363	0.16875268998600668	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	9259	0.1668782608758342	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8397	0.15134212728959712	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	8279	0.14921537118382455	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	8236	0.14844036684019554	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	8046	0.1450159290427651	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7987	0.1439525509898788	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7985	0.14391650427622163	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7976	0.14375429406476442	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7961	0.1434839437123357	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7839	0.14128509417924878	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7625	0.13742809581793236	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7489	0.13497691928924532	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	7240	0.13048910343892858	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	7118	0.12829025390584167	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7086	0.12771350648732707	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6918	0.12468558254012539	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6903	0.12441523218769668	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	6708	0.12090067760612332	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6687	0.12052218711272311	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6675	0.12030590683078016	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6515	0.11742216973820714	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6385	0.11507913335049157	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6318	0.11387156844297663	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6245	0.11255586339449018	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6229	0.11226748968523288	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	6077	0.10952793944728853	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6041	0.1088790986014596	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	5868	0.10576105787011504	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	5859	0.1055988476586578	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5836	0.10518431045160044	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5759	0.10379651197579967	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5653	0.10188603615197006	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5650	0.10183196608148432	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	5560	0.100209863966912	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	7.209342731432518E-5
7	0.0	0.0	0.0	0.0	7.209342731432518E-5
8	0.0	0.0	0.0	0.0	7.209342731432518E-5
9	0.0	0.0	0.0	0.0	7.209342731432518E-5
10-11	0.0	0.0	0.0	0.0	7.209342731432518E-5
12-13	0.0	0.0	0.0	0.0	7.209342731432518E-5
14-15	0.0	0.0	0.0	0.0	9.011678414290648E-5
16-17	0.0	0.0	0.0	0.0	1.1715181938577842E-4
18-19	0.0	0.0	0.0	0.0	1.2616349780006906E-4
20-21	0.0	0.0	0.0	9.011678414290647E-6	1.2616349780006906E-4
22-23	0.0	0.0	0.0	5.4070070485743885E-5	1.53198533042941E-4
24-25	0.0	0.0	0.0	1.6221021145723164E-4	1.8023356828581296E-4
26-27	0.0	0.0	0.0	4.59595599128823E-4	1.8023356828581296E-4
28-29	0.0	0.0	0.0	0.0014508802247007943	1.8023356828581296E-4
30-31	0.0	0.0	0.0	0.006227069784274838	1.9825692511439425E-4
32-33	0.0	0.0	0.0	0.01725736416336659	2.523269956001381E-4
34-35	0.0	0.0	0.0	0.03469496189501899	2.883737092573007E-4
36-37	0.0	0.0	0.0	0.06747944796620836	3.06397066085882E-4
38-39	0.0	0.0	0.0	0.11802595219196461	3.06397066085882E-4
40-41	0.0	0.0	0.0	0.1761242429288964	3.06397066085882E-4
42-43	0.0	0.0	0.0	0.24003506624304566	3.244204229144633E-4
44-45	0.0	0.0	0.0	0.31493112554421526	3.424437797430446E-4
46-47	0.0	0.0	0.0	0.412743883052926	4.0552552864307913E-4
48-49	0.0	0.0	0.0	0.5307428002096477	4.505839207145324E-4
50-51	0.0	0.0	0.0	0.6570504848643455	4.505839207145324E-4
52-53	0.0	0.0	0.0	0.7854218438759157	5.316890264431482E-4
54-55	0.0	0.0	0.0	0.9283560752049796	6.12794132171764E-4
56-57	0.0	0.0	0.0	1.1032276948342896	6.12794132171764E-4
58-59	0.0	0.0	0.0	1.321454499314752	6.12794132171764E-4
60-61	0.0	0.0	0.0	1.5453316261609746	6.308174890003453E-4
62-63	0.0	0.0	0.0	1.7840960457476052	6.668642026575079E-4
64-65	0.0	0.0	0.0	2.0397934090746883	6.668642026575079E-4
66-67	0.0	0.0	0.0	2.3271848453848314	6.668642026575079E-4
68-69	0.0	0.0	0.0	2.656201224290583	6.668642026575079E-4
70-71	0.0	0.0	0.0	3.0188041286463956	6.848875594860892E-4
72-73	0.0	0.0	0.0	3.3996556817911467	8.831444846004834E-4
74-75	0.0	0.0	0.0	3.7737935345172513	8.831444846004834E-4
76-77	0.0	0.0	0.0	4.208336667654347	8.831444846004834E-4
78-79	0.0	0.0	0.0	4.676322139386874	8.831444846004834E-4
80-81	0.0	0.0	0.0	5.185725285111482	9.011678414290647E-4
82-83	0.0	0.0	0.0	5.7180721640788725	9.372145550862273E-4
84-85	0.0	1.8023356828581294E-5	0.0	6.237649494733215	9.46226233500518E-4
86-87	0.0	1.8023356828581294E-5	0.0	6.799203223441323	9.552379119148086E-4
88	0.0	1.8023356828581294E-5	0.0	7.248867952957597	9.552379119148086E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6030	0.0	41.795727	94
TATTCTC	3045	0.0	36.409595	5
GTACTAG	7070	0.0	33.087334	1
TACCCCG	425	0.0	32.05533	5
CAACGCA	11365	0.0	30.753508	5
GTATCAA	13120	0.0	30.051903	1
TAGGCAT	8435	0.0	29.963213	5
GCATCGT	7320	0.0	29.312721	8
CGTTAAC	1575	0.0	28.746845	1
ATCAACG	12360	0.0	28.204597	3
TAGTACT	9750	0.0	27.560713	4
CTAGGCA	9820	0.0	27.172894	4
CTGTGCG	9825	0.0	26.954607	9
AGGGCTA	7345	0.0	26.798677	5
ACTGTGC	10355	0.0	25.890297	8
TATCAAC	15160	0.0	25.81572	2
GGTATCA	8855	0.0	25.725159	1
TTCTCTG	4335	0.0	25.560541	7
TCTCTGC	4330	0.0	25.481853	8
GTACTGT	10545	0.0	25.288857	6
>>END_MODULE