##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5548356
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84695502595724	31.0	31.0	34.0	30.0	34.0
2	31.851902617640253	31.0	31.0	34.0	30.0	34.0
3	32.028468793278584	33.0	31.0	34.0	30.0	34.0
4	35.75202330203758	37.0	35.0	37.0	35.0	37.0
5	35.656417684806094	37.0	35.0	37.0	33.0	37.0
6	35.68234103939978	37.0	35.0	37.0	33.0	37.0
7	35.60341243424178	37.0	35.0	37.0	33.0	37.0
8	35.59239890158454	37.0	35.0	37.0	33.0	37.0
9	37.19232742095136	39.0	37.0	39.0	33.0	39.0
10-11	37.134545169776416	39.0	37.0	39.0	33.0	39.0
12-13	37.05732454802828	39.0	37.0	39.0	33.0	39.0
14-15	38.250555479857454	40.0	38.0	41.0	33.0	41.0
16-17	38.25981543001206	40.0	37.5	41.0	33.0	41.0
18-19	38.30637291478773	40.0	38.0	41.0	33.0	41.0
20-21	38.31867322500575	40.0	38.0	41.0	33.0	41.0
22-23	38.284405236434	40.0	38.0	41.0	33.0	41.0
24-25	38.2053692120693	40.0	37.0	41.0	33.0	41.0
26-27	38.057617427576744	40.0	37.0	41.0	33.0	41.0
28-29	37.99940450829038	40.0	37.0	41.0	33.0	41.0
30-31	37.87851049932629	40.0	37.0	41.0	33.0	41.0
32-33	37.75825640964639	40.0	36.5	41.0	33.0	41.0
34-35	37.66136311368629	40.0	36.0	41.0	32.0	41.0
36-37	37.58195571805415	40.0	36.0	41.0	32.0	41.0
38-39	37.465706418261554	40.0	36.0	41.0	32.0	41.0
40-41	37.33231888869423	40.0	36.0	41.0	31.0	41.0
42-43	37.207225888173	39.0	35.5	41.0	31.0	41.0
44-45	36.9912901226958	39.0	35.0	41.0	31.0	41.0
46-47	36.745953756391984	39.0	35.0	41.0	30.5	41.0
48-49	36.47015782693108	39.0	35.0	41.0	30.0	41.0
50-51	36.255035095080416	38.5	35.0	41.0	29.5	41.0
52-53	36.042266934565845	38.0	35.0	40.0	29.0	41.0
54-55	35.82425902375407	38.0	34.5	40.0	29.0	41.0
56-57	35.537417389943975	37.0	34.0	40.0	28.5	41.0
58-59	35.22648573739681	37.0	34.0	40.0	28.0	41.0
60-61	34.95608140501439	36.0	34.0	39.5	28.0	41.0
62-63	34.66774896924422	36.0	34.0	39.0	27.5	41.0
64-65	34.38441350915478	35.0	33.0	39.0	27.0	41.0
66-67	34.16391828498388	35.0	33.0	38.5	27.0	41.0
68-69	33.977971582933755	35.0	33.0	38.0	26.0	40.5
70-71	33.80607724161896	35.0	33.0	37.0	26.0	40.0
72-73	33.623861104081996	35.0	33.0	37.0	26.0	40.0
74-75	33.45235390447188	35.0	33.0	36.0	26.5	39.0
76-77	33.32082106843902	35.0	33.0	36.0	27.0	39.0
78-79	33.22645347558809	35.0	33.0	36.0	27.0	39.0
80-81	33.10599860571311	35.0	33.0	35.0	27.0	38.0
82-83	32.92439102681948	35.0	33.0	35.0	27.0	37.0
84-85	32.79491645813643	35.0	33.0	35.0	27.0	37.0
86-87	32.67590300622383	35.0	33.0	35.0	27.0	36.0
88-89	32.578771982187156	35.0	33.0	35.0	27.0	36.0
90-91	32.491479728409644	35.0	33.0	35.0	26.0	36.0
92-93	32.41192093658013	35.0	33.0	35.0	26.0	36.0
94-95	32.38008772328236	35.0	33.0	35.0	26.0	35.0
96-97	32.332589329163454	35.0	33.0	35.0	26.0	35.0
98-99	32.29553619126098	35.0	33.0	35.0	26.0	35.0
100	31.782567665088543	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.49641035677417733
1101	2	0.5962926099728243
1101	3	0.4604526333076606
1101	4	0.33335060005947525
1101	5	0.3852226457815817
1101	6	0.3854241755164409
1101	7	0.3972175974631469
1101	8	0.4092659371004359
1101	9	0.5465840352504898
1101	10-11	0.5425461565962593
1101	12-13	0.5571152436640006
1101	14-15	0.6386542723426203
1101	16-17	0.6714153880552658
1101	18-19	0.654393142319087
1101	20-21	0.6207272678631028
1101	22-23	0.6253060424081909
1101	24-25	0.6776019271041207
1101	26-27	0.6845094082173446
1101	28-29	0.6746821164320593
1101	30-31	0.7500136151209773
1101	32-33	0.7488263192222107
1101	34-35	0.7707129846574503
1101	36-37	0.8112730362053142
1101	38-39	0.8230406518231348
1101	40-41	0.7818222573069065
1101	42-43	0.7865285571175846
1101	44-45	0.7558629816575859
1101	46-47	0.8146155579266932
1101	48-49	0.7980103877963813
1101	50-51	0.763061540823216
1101	52-53	0.7691212629721065
1101	54-55	0.7451658691819105
1101	56-57	0.6917290521759014
1101	58-59	0.7118067118447513
1101	60-61	0.7444998091968458
1101	62-63	0.7142146227290596
1101	64-65	0.702675077257183
1101	66-67	0.6702247705044968
1101	68-69	0.6976316535136036
1101	70-71	0.6321733347036655
1101	72-73	0.6724812136430458
1101	74-75	0.6825198249708819
1101	76-77	0.6553264631074285
1101	78-79	0.6223199412750731
1101	80-81	0.625960926072402
1101	82-83	0.5611737758584567
1101	84-85	0.5804943006630126
1101	86-87	0.5503562847719508
1101	88-89	0.5587629028483505
1101	90-91	0.5516085753646962
1101	92-93	0.5846383555625181
1101	94-95	0.5465792189529068
1101	96-97	0.5778594051991774
1101	98-99	0.5928406155415615
1101	100	0.6863381432906124
1102	1	0.44192118271826075
1102	2	0.5686907543554547
1102	3	0.4656613111982537
1102	4	0.3562625812865221
1102	5	0.3010655879048727
1102	6	0.2996227161102638
1102	7	0.3427557651710842
1102	8	0.3456898816429401
1102	9	0.3947233793442919
1102	10-11	0.4540107508089761
1102	12-13	0.4767742953188048
1102	14-15	0.5844154292413606
1102	16-17	0.615671490105882
1102	18-19	0.6126092345427736
1102	20-21	0.5816694431951106
1102	22-23	0.5658743342135182
1102	24-25	0.6093201988343893
1102	26-27	0.6278637855086728
1102	28-29	0.6305956856952051
1102	30-31	0.6561421018607234
1102	32-33	0.6782519078643645
1102	34-35	0.6206693700877679
1102	36-37	0.6432956755307941
1102	38-39	0.6379548868050477
1102	40-41	0.5416773028326745
1102	42-43	0.6453793965830457
1102	44-45	0.6052764634472112
1102	46-47	0.5800435363137595
1102	48-49	0.5821302999749847
1102	50-51	0.5737179214090702
1102	52-53	0.48106218894938024
1102	54-55	0.5633321568445808
1102	56-57	0.5441855558428159
1102	58-59	0.5773747628643093
1102	60-61	0.5850891032718906
1102	62-63	0.5321801453685993
1102	64-65	0.5533125054868435
1102	66-67	0.5498688488778924
1102	68-69	0.5248066211081479
1102	70-71	0.5348014790963589
1102	72-73	0.5250732904828865
1102	74-75	0.5358564186154879
1102	76-77	0.5212918394067216
1102	78-79	0.4841657676525628
1102	80-81	0.47894119270778646
1102	82-83	0.4385511831951092
1102	84-85	0.44957568927386404
1102	86-87	0.5063365049752093
1102	88-89	0.4761201359308487
1102	90-91	0.4554046026437817
1102	92-93	0.47864872674493597
1102	94-95	0.4568130312846179
1102	96-97	0.4956952485265589
1102	98-99	0.47112017170797316
1102	100	0.5440917255720024
1103	1	0.25555972629762636
1103	2	-0.12575204573888143
1103	3	0.17788339544535603
1103	4	0.19851088384476867
1103	5	0.2024930548701036
1103	6	0.24445684834833514
1103	7	0.28532554816818845
1103	8	0.30361326938083977
1103	9	0.3628461807854393
1103	10-11	0.3729606787413502
1103	12-13	0.37499576400179535
1103	14-15	0.4970392561190664
1103	16-17	0.4827664520201935
1103	18-19	0.506252635869707
1103	20-21	0.48527538547511995
1103	22-23	0.4654802033970924
1103	24-25	0.44663280827219864
1103	26-27	0.49562467324477666
1103	28-29	0.532859538513506
1103	30-31	0.5415283395568125
1103	32-33	0.5473620292317847
1103	34-35	0.4992514588990815
1103	36-37	0.5303551094103867
1103	38-39	0.5007100426580138
1103	40-41	0.5315238909628732
1103	42-43	0.5173411999527602
1103	44-45	0.4768489491419601
1103	46-47	0.5228924239600659
1103	48-49	0.5687927396146648
1103	50-51	0.5406740864245663
1103	52-53	0.5275843884672611
1103	54-55	0.4960852967898717
1103	56-57	0.4843030252728937
1103	58-59	0.4529488828688031
1103	60-61	0.5561070632682856
1103	62-63	0.5096794284335573
1103	64-65	0.48341063313884547
1103	66-67	0.46745287344981534
1103	68-69	0.41020744931382325
1103	70-71	0.4089264765962568
1103	72-73	0.40862177147990053
1103	74-75	0.42414070706842466
1103	76-77	0.39619599026280383
1103	78-79	0.32169606921998906
1103	80-81	0.35124085126971494
1103	82-83	0.367776901975553
1103	84-85	0.3911350363154682
1103	86-87	0.4148186703795034
1103	88-89	0.40377566263313724
1103	90-91	0.40810478220489443
1103	92-93	0.3775210127338866
1103	94-95	0.39402345679366135
1103	96-97	0.4077215264084231
1103	98-99	0.4347383979308006
1103	100	0.3403211004693887
1104	1	0.34814279952539806
1104	2	0.4066976679762213
1104	3	0.2922696392186239
1104	4	0.21061821399808167
1104	5	0.22639255321662688
1104	6	0.24395471502799637
1104	7	0.25655826635961176
1104	8	0.2638665673106928
1104	9	0.2732874850855964
1104	10-11	0.24222829222742348
1104	12-13	0.308555692005271
1104	14-15	0.39106869035467184
1104	16-17	0.38045552562257257
1104	18-19	0.38144124622836983
1104	20-21	0.4218473601588215
1104	22-23	0.37958344863628213
1104	24-25	0.35274104711413656
1104	26-27	0.4506770037443033
1104	28-29	0.4462888415511159
1104	30-31	0.46608504697721287
1104	32-33	0.4838218266789269
1104	34-35	0.4764067920381265
1104	36-37	0.47113587859973904
1104	38-39	0.4981008408661509
1104	40-41	0.4627498015220439
1104	42-43	0.43074835210108375
1104	44-45	0.4703836374066981
1104	46-47	0.4804338839246256
1104	48-49	0.5138835724886519
1104	50-51	0.48842182640616016
1104	52-53	0.4705268780073837
1104	54-55	0.4630671365757806
1104	56-57	0.49628519174257235
1104	58-59	0.4950391155485505
1104	60-61	0.4664164805040514
1104	62-63	0.4960598397716609
1104	64-65	0.3903522643180537
1104	66-67	0.37354885118867287
1104	68-69	0.455145605648994
1104	70-71	0.39184041324951124
1104	72-73	0.4864078135294321
1104	74-75	0.44336539134990005
1104	76-77	0.35398793103277626
1104	78-79	0.3150633843609043
1104	80-81	0.3519615642091978
1104	82-83	0.357040109275907
1104	84-85	0.3520275296803774
1104	86-87	0.315074568946514
1104	88-89	0.3584469783083648
1104	90-91	0.33654004338536225
1104	92-93	0.39660029344484826
1104	94-95	0.3589266560125708
1104	96-97	0.3097393134051387
1104	98-99	0.3503799443173037
1104	100	0.3546142826097878
1105	1	0.12193954289878306
1105	2	-0.39725474857725374
1105	3	0.03411475487337157
1105	4	0.12257157870225655
1105	5	0.15975232855912225
1105	6	0.22919166557839787
1105	7	0.29788909046797585
1105	8	0.19421903431419452
1105	9	0.28654239960682304
1105	10-11	0.26490402994149065
1105	12-13	0.29083673847820535
1105	14-15	0.3529572500091973
1105	16-17	0.3426566043791084
1105	18-19	0.3068383673131265
1105	20-21	0.3103446712886182
1105	22-23	0.32423859477401606
1105	24-25	0.35911965630317866
1105	26-27	0.3038158721424793
1105	28-29	0.35995360721434366
1105	30-31	0.4074216794767054
1105	32-33	0.40946532035427907
1105	34-35	0.43249296256308867
1105	36-37	0.45655833809934876
1105	38-39	0.45856276285171305
1105	40-41	0.4451875639195748
1105	42-43	0.4455503730892971
1105	44-45	0.4182710702333452
1105	46-47	0.3996452758944997
1105	48-49	0.4489894608419718
1105	50-51	0.42360051326168957
1105	52-53	0.3823169439436853
1105	54-55	0.4059471415765259
1105	56-57	0.4792612398736651
1105	58-59	0.4433587479935639
1105	60-61	0.4068124993774518
1105	62-63	0.36752206628287354
1105	64-65	0.3824985588825882
1105	66-67	0.38558703223290536
1105	68-69	0.3824525948860398
1105	70-71	0.3507771900183769
1105	72-73	0.32021964765616673
1105	74-75	0.30486473348045706
1105	76-77	0.28696012073261556
1105	78-79	0.25868317094955984
1105	80-81	0.31412119594613586
1105	82-83	0.3085988740136614
1105	84-85	0.32247056333115864
1105	86-87	0.34202109219570076
1105	88-89	0.3200909493781481
1105	90-91	0.3156129771401126
1105	92-93	0.2514701315484018
1105	94-95	0.25978725213605003
1105	96-97	0.24728592920136094
1105	98-99	0.25378595721911523
1105	100	0.2386236158645758
1106	1	0.2632810794053597
1106	2	0.2884617538751222
1106	3	0.25003716143101684
1106	4	0.17830959457841544
1106	5	0.17138605929225292
1106	6	0.18600635813400146
1106	7	0.19869946158777907
1106	8	0.21906534710521441
1106	9	0.2586815578834205
1106	10-11	0.2575787859163796
1106	12-13	0.2306946871060589
1106	14-15	0.3458067711306825
1106	16-17	0.30574583039723535
1106	18-19	0.4005249390261483
1106	20-21	0.3651722901060168
1106	22-23	0.3283439451936232
1106	24-25	0.3154762799253348
1106	26-27	0.3304066296995174
1106	28-29	0.36887771046954043
1106	30-31	0.3111829414816185
1106	32-33	0.363309610070921
1106	34-35	0.34414333144907516
1106	36-37	0.3208353407208264
1106	38-39	0.3191126833568063
1106	40-41	0.3105911169159228
1106	42-43	0.3741984596473671
1106	44-45	0.38232276142107224
1106	46-47	0.34398268540542887
1106	48-49	0.3831904239117989
1106	50-51	0.40192382633709656
1106	52-53	0.35809618221929185
1106	54-55	0.275136645449777
1106	56-57	0.32567625804519196
1106	58-59	0.3348842757029118
1106	60-61	0.3771000805759144
1106	62-63	0.3192647922033771
1106	64-65	0.34081512837673245
1106	66-67	0.3167636152794344
1106	68-69	0.35369840188808865
1106	70-71	0.3112254812345583
1106	72-73	0.3213757920669025
1106	74-75	0.306567925954063
1106	76-77	0.27909235335260973
1106	78-79	0.29627502218150426
1106	80-81	0.2766433252329463
1106	82-83	0.24540228469232517
1106	84-85	0.2155560962092764
1106	86-87	0.29580844260688366
1106	88-89	0.26341610834418105
1106	90-91	0.1866149186882211
1106	92-93	0.22092969118968142
1106	94-95	0.27144677234579717
1106	96-97	0.2858770818890619
1106	98-99	0.2613684641817997
1106	100	0.2654789805173081
1107	1	0.05958993237765853
1107	2	-0.5364016750716729
1107	3	-0.04321717266302372
1107	4	0.033072417495361606
1107	5	0.1070316763427428
1107	6	0.16890385812808972
1107	7	0.1610266813140555
1107	8	0.17195026267792457
1107	9	0.1743066138724103
1107	10-11	0.23262959035218245
1107	12-13	0.1687066419255956
1107	14-15	0.2560794899753418
1107	16-17	0.294035368364959
1107	18-19	0.2831853402967184
1107	20-21	0.2411871012208593
1107	22-23	0.2086388504088461
1107	24-25	0.2244545833920597
1107	26-27	0.2774246204479667
1107	28-29	0.25811698606508315
1107	30-31	0.28418287041693446
1107	32-33	0.33202102776889575
1107	34-35	0.3665326351758935
1107	36-37	0.36850866169539387
1107	38-39	0.38473935663854064
1107	40-41	0.3667993145580013
1107	42-43	0.37533842056219413
1107	44-45	0.41863092338650176
1107	46-47	0.3819195834122979
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2216	76-77	-0.8273938281595079
2216	78-79	-0.7485133779804514
2216	80-81	-0.7224915024764158
2216	82-83	-0.601998388352726
2216	84-85	-0.6684025114306493
2216	86-87	-0.6148079345441886
2216	88-89	-0.6415888549080577
2216	90-91	-0.6889128732866183
2216	92-93	-0.7072719168025969
2216	94-95	-0.6597559995567366
2216	96-97	-0.7070330308828368
2216	98-99	-0.6786805208950213
2216	100	-0.7524773052693519
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	9.0
13	54.0
14	286.0
15	926.0
16	2350.0
17	4677.0
18	8762.0
19	13136.0
20	19048.0
21	27685.0
22	37284.0
23	43920.0
24	46258.0
25	49338.0
26	55455.0
27	68417.0
28	86783.0
29	107111.0
30	133600.0
31	164380.0
32	205085.0
33	261360.0
34	359382.0
35	497448.0
36	746193.0
37	1270568.0
38	956427.0
39	381764.0
40	649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87907620304613	22.22604768237801	13.431294755630606	24.463581358945248
2	15.00910312786754	20.29491935337061	40.12376865348147	24.572208865280377
3	17.843728681283327	27.158691007140106	29.771089364455545	25.22649094712102
4	12.245651097314715	17.366891785062503	39.53257149676961	30.854885620853175
5	12.609760942029064	38.89503503302156	35.135559427085646	13.359644597863737
6	28.957009968358193	39.441791406319275	17.947009889055423	13.654188736267104
7	25.5779843102452	32.43964764918623	24.480087175717973	17.502280864850604
8	26.274071094212413	34.7356406113811	19.7571316620635	19.233156632342986
9	26.275693196326984	14.154985729106063	22.061111435531533	37.508209639035414
10-11	25.862408612569197	25.855739970542622	27.129756994684552	21.15209442220362
12-13	26.084699684014506	23.71091544954938	27.71848994549016	22.485894920945952
14-15	23.410092521994926	24.534794434620856	25.479481314450407	26.575631728933807
16-17	21.742463676481506	26.94362275593725	27.000515122392937	24.313398445188312
18-19	21.135179501819998	27.265914443846068	29.545265300207845	22.053640754126086
20-21	22.831317945281956	26.472678150856492	28.49371953205929	22.20228437180226
22-23	23.426455107894594	25.94267243791509	28.24931238052102	22.3815600736693
24-25	22.84746148228412	26.05934983263511	29.127213538568903	21.96597514651187
26-27	22.759988043284608	26.26981750328679	27.97314479187628	22.99704966155232
28-29	21.704546355713298	27.04167684986328	28.630561917800517	22.623214876622914
30-31	22.7586604031897	26.446158105211705	28.489673337471494	22.305508154127097
32-33	23.411880924727974	26.77866200366379	27.38244445742126	22.427012614186978
34-35	23.138999995944722	26.504068117824698	28.490991204106102	21.865940682124478
36-37	22.878650901275982	26.006081801528236	28.130098357062884	22.985168940132898
38-39	23.101852152241133	25.88476658671506	28.908851558912225	22.104529702131586
40-41	22.443755368169825	25.147641285543692	29.50401557802218	22.904587768264303
42-43	22.716429545019317	25.43430998163856	28.446451060570215	23.4028094127719
44-45	22.844037044486694	26.53832955203307	28.10750607927826	22.51012732420198
46-47	22.92808896905678	26.259355023361874	27.338872992288167	23.47368301529318
48-49	23.983127149354992	25.898273919418692	27.390418886144214	22.7281800450821
50-51	23.256132086693786	25.949731776403677	27.03659426323761	23.75754187366492
52-53	22.42667918208565	26.632492579783996	26.950289419063957	23.990538819066405
54-55	22.593817385116825	26.62171261561132	26.667555222001855	24.116914777269997
56-57	23.9325626112212	25.864489537558622	26.497281667610338	23.70566618360984
58-59	22.477025114354003	26.3929884626774	27.677354365926682	23.452632057041917
60-61	22.69502440409992	27.16493848036457	26.62339915259499	23.516637962940518
62-63	23.294792187091097	26.151476221064403	26.087340105789895	24.466391486054608
64-65	22.23763219231066	26.43629031734806	26.694970546230273	24.63110694411101
66-67	23.023702877032402	25.966151054474512	26.699494408794244	24.310651659698838
68-69	22.338428552452267	25.850719674392376	27.18753204826078	24.623319724894575
70-71	22.39010133636321	25.50892799773336	27.30383669461545	24.797133971287984
72-73	23.101816105527476	25.590282959492868	27.674233592797577	23.63366734218208
74-75	23.217427257110817	26.121573594134322	27.127243214776016	23.53375593397885
76-77	22.82820838428453	26.177798336992623	26.47806681138023	24.515926467342616
78-79	22.493056688905508	26.267907317261756	26.784838056094586	24.45419793773815
80-81	22.06373571568965	26.739082651750536	27.521150417619193	23.676031214940622
82-83	23.1084028687975	25.37115320007325	28.695626078718906	22.824817852410344
84-85	23.51756228884374	26.084061640685043	26.835094801320253	23.563281269150963
86-87	21.768852969030117	26.88846025869941	27.434612266321746	23.908074505948733
88-89	22.843763580542543	25.432013336892627	27.261645312413535	24.462577770151295
90-91	22.426170766250497	25.414821226842538	27.568138016743802	24.59086999016316
92-93	22.841943161641467	25.84709533260194	27.144605694939244	24.16635581081735
94-95	21.349199182663998	25.804057085191793	28.02440725206366	24.822336480080544
96-97	21.811481231512218	25.699927876073374	27.48388431729517	25.004706575119233
98-99	21.06752012619493	26.61593528184243	27.353421137722545	24.963123454240097
100	22.072302187991895	25.63728190702757	26.435117690203263	25.85529821477727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	67.0
2	68.0
3	108.5
4	73.5
5	51.0
6	123.0
7	302.5
8	488.5
9	545.0
10	440.0
11	328.5
12	353.5
13	425.0
14	576.5
15	940.0
16	1318.0
17	1786.0
18	2571.5
19	3515.5
20	4570.0
21	5690.5
22	6804.5
23	7857.0
24	8850.5
25	10071.5
26	11816.5
27	13493.5
28	17585.0
29	27978.5
30	43203.5
31	56979.0
32	76404.5
33	95623.5
34	107734.5
35	123051.5
36	137478.5
37	168074.0
38	181605.0
39	164180.5
40	148359.0
41	132326.0
42	127365.0
43	135789.5
44	152987.5
45	193027.0
46	231142.5
47	257994.0
48	275140.0
49	266313.5
50	252409.0
51	258343.0
52	263409.0
53	255467.5
54	247129.5
55	213567.0
56	183612.5
57	150262.5
58	115226.0
59	113021.5
60	93881.5
61	61504.0
62	44204.0
63	36796.5
64	26739.5
65	11399.5
66	5149.5
67	3403.5
68	2463.5
69	1841.0
70	1424.5
71	1079.5
72	764.0
73	566.5
74	402.0
75	275.5
76	191.5
77	100.0
78	44.0
79	23.5
80	12.0
81	5.5
82	2.0
83	2.0
84	2.0
85	0.5
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013157050484864345
2	0.004740142845916881
3	0.0021808261762583368
4	0.026980965172386198
5	0.00291978380623017
6	0.0
7	3.965138502287885E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.00875935141869051
16-17	0.002883737092573007
18-19	0.0
20-21	6.308174890003453E-5
22-23	8.561094493576115E-4
24-25	0.0
26-27	1.712218898715223E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.136656696145669E-4
36-37	0.0
38-39	0.0
40-41	4.7761895595740435E-4
42-43	7.840160220432863E-4
44-45	0.0
46-47	0.0
48-49	6.218058105860547E-4
50-51	0.0
52-53	0.0
54-55	4.325605638859511E-4
56-57	1.712218898715223E-4
58-59	2.7035035242871944E-4
60-61	9.912846255719713E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0014959386167722475
70-71	9.282028766719367E-4
72-73	0.0
74-75	2.343036387715568E-4
76-77	0.00278460863001581
78-79	5.497123832717295E-4
80-81	0.003235192550730343
82-83	0.00194652253748678
84-85	0.00598375446708899
86-87	0.008110510572861584
88-89	0.007876206934090026
90-91	0.006010789502331862
92-93	0.009020690092704937
94-95	0.0015680320440865725
96-97	0.0048032245948169145
98-99	0.0020546626784582675
100	0.08532257122650384
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5548356.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.378022346978822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.359418823471415	9.109831397354625
2	15.633284345205828	4.495514233440872
3	6.834404478239704	2.9479566094926786
4	3.860682716726891	2.220359295027766
5	2.3049009421046587	1.6569958626576664
6	1.5617669952110034	1.3473072454750668
7	1.062981595148851	1.0698501190634162
8	0.7761136091848349	0.8927183053323154
9	0.5666373965245015	0.7332412634876866
>10	2.9962334090426297	7.949778706036606
>50	0.34304197518879637	3.5155479244335734
>100	0.48114546564583976	15.396337443809674
>500	0.10994485357911114	10.986108929489212
>1k	0.10154541778686411	27.788496022928555
>5k	0.006644329805807158	6.813239060841765
>10k+	0.001253647133171162	3.076717581128558
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	26603	0.4794753617107482	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	21197	0.38204109469543773	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	19809	0.3570246754173669	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	18957	0.3416687753994156	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	18543	0.33420710567238293	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	18223	0.3284396314872369	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	15457	0.2785870264993811	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	11283	0.20335753509688276	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	10529	0.18976792404813245	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	10091	0.18187369375721385	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9959	0.1794946106558411	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	9677	0.1744120240301812	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9650	0.1739253933958095	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	9509	0.17138410008297955	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	9361	0.1687166432723495	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	9130	0.1645532478449472	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	9122	0.16440906099031857	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	8891	0.16024566556291628	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8846	0.15943461450563012	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	8806	0.1587136802324869	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8799	0.15858751673468682	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	8692	0.1566590175540286	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	8390	0.15121596379179708	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	8333	0.15018863245256794	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	8327	0.15008049231159645	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8252	0.14872874054945284	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	8201	0.1478095493511952	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	8052	0.1451240691837366	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7742	0.13953682856687638	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7625	0.13742809581793236	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	7513	0.13540947985313126	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	7188	0.12955188888384234	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	7129	0.12848851083095605	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6968	0.12558675038155445	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6954	0.12533442338595432	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6893	0.12423499861941086	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6876	0.12392860155332498	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	6788	0.12234254615240982	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	6632	0.11953090248715115	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	6619	0.11929659884837959	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6497	0.11709774931529268	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	6486	0.11689949239017827	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6445	0.11616053476020645	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	6256	0.11275412031960458	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	6213	0.1119791159759756	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	6177	0.11133027513014666	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6119	0.11028492043408894	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	6114	0.11019480364994604	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	6102	0.10997852336800307	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	6064	0.10929363580851696	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5833	0.1051302403811147	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5822	0.10493198345600031	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	5692	0.10258894706828474	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	5642	0.10168777922685568	No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG	5565	0.10029998075105492	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.8023356828581294E-5
5	0.0	0.0	0.0	0.0	1.8023356828581294E-5
6	0.0	0.0	0.0	0.0	1.8023356828581294E-5
7	0.0	0.0	0.0	0.0	1.8023356828581294E-5
8	0.0	0.0	0.0	0.0	1.8023356828581294E-5
9	0.0	0.0	0.0	0.0	5.4070070485743885E-5
10-11	0.0	0.0	0.0	0.0	5.4070070485743885E-5
12-13	0.0	0.0	0.0	0.0	5.4070070485743885E-5
14-15	0.0	0.0	0.0	0.0	7.209342731432519E-5
16-17	0.0	0.0	0.0	0.0	9.011678414290648E-5
18-19	0.0	0.0	0.0	0.0	1.4418685462865035E-4
20-21	0.0	0.0	0.0	1.8023356828581294E-5	1.4418685462865035E-4
22-23	0.0	0.0	0.0	7.209342731432519E-5	1.53198533042941E-4
24-25	0.0	0.0	0.0	1.8023356828581296E-4	1.8023356828581296E-4
26-27	0.0	0.0	0.0	4.86630634371695E-4	1.8023356828581296E-4
28-29	0.0	0.0	0.0	0.0015139619736008287	1.8023356828581296E-4
30-31	0.0	0.0	0.0	0.0063081748900034535	1.9825692511439425E-4
32-33	0.0	0.0	0.0	0.017572772907866765	2.3430363877155683E-4
34-35	0.0	0.0	0.0	0.03521763924304785	2.523269956001381E-4
36-37	0.0	0.0	0.0	0.06836259245080886	2.6133867401442875E-4
38-39	0.0	0.0	0.0	0.11903526017436517	2.7936203084301007E-4
40-41	0.0	0.0	0.0	0.17762018154566867	2.883737092573007E-4
42-43	0.0	0.0	0.0	0.24244118437966128	2.883737092573007E-4
44-45	0.0	0.0	0.0	0.31886922901126025	2.883737092573007E-4
46-47	0.0	0.0	0.0	0.4180427499605288	3.1540874450017265E-4
48-49	0.0	0.0	0.0	0.53763673419658	3.2442042291446334E-4
50-51	0.0	0.0	0.0	0.6653592523623214	3.2442042291446334E-4
52-53	0.0	0.0	0.0	0.7954608536294354	3.514554581573353E-4
54-55	0.0	0.0	0.0	0.9396206732228429	3.784904934002072E-4
56-57	0.0	0.0	0.0	1.1159341613984395	3.9651385022878845E-4
58-59	0.0	0.0	0.0	1.3360624300243171	4.1453720705736976E-4
60-61	0.0	0.0	0.0	1.562093348011555	4.1453720705736976E-4
62-63	0.0	0.0	0.0	1.8032098156643157	4.505839207145324E-4
64-65	0.0	0.0	0.0	2.062115336506886	4.505839207145324E-4
66-67	0.0	0.0	0.0	2.3527149303325166	4.505839207145324E-4
68-69	0.0	0.0	0.0	2.6844348127625555	4.505839207145324E-4
70-71	0.0	0.0	0.0	3.0492275549730405	4.505839207145324E-4
72-73	0.0	0.0	0.0	3.4334494758447365	5.226773480288575E-4
74-75	0.0	0.0	0.0	3.811408280218501	5.226773480288575E-4
76-77	0.0	0.0	0.0	4.253061627624471	5.226773480288575E-4
78-79	0.0	0.0	0.0	4.730752677009189	5.226773480288575E-4
80-81	0.0	0.0	0.0	5.251717806139332	5.677357401003108E-4
82-83	0.0	0.0	0.0	5.7949507926311865	7.029109163146705E-4
84-85	0.0	0.0	0.0	6.324224689259305	7.029109163146705E-4
86-87	0.0	0.0	0.0	6.895565100725332	7.029109163146705E-4
88	0.0	0.0	0.0	7.353493539347511	7.029109163146705E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5710	0.0	41.025497	94
GGTATCA	11705	0.0	39.08641	1
GTATCAA	19445	0.0	36.271683	1
TATTCTC	3070	0.0	33.985023	5
CAACGCA	21320	0.0	30.905357	5
ATCAACG	22010	0.0	29.98812	3
TAGGCAT	8445	0.0	29.049843	5
GTGGTAT	3135	0.0	28.947039	1
TATCAAC	24750	0.0	28.436243	2
TATCCGC	220	3.6379788E-12	27.784655	1
CTGTGCG	9075	0.0	27.60581	9
TAGTACT	9170	0.0	27.221966	4
ACTGTGC	9530	0.0	26.974638	8
AACGCAG	26025	0.0	26.708075	6
TACCCCG	405	0.0	26.689827	5
AGGGCTA	7480	0.0	26.640184	5
CTAGGCA	9645	0.0	26.51496	4
TACTGTG	10725	0.0	25.502974	7
ACGCAGA	25885	0.0	25.47268	7
CGCAGAG	25915	0.0	25.424828	8
>>END_MODULE