FastQCFastQC Report
Mon 16 Jan 2017
ERR1146437_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146437_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5549712
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC217880.39259694917502025No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT178930.3224131270235284No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170530.30727720645683954No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA141860.255616868046486No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC130800.23568790596701236No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA127050.2289307985711691No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC118110.21282185453947883No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC104900.189018817553055No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG97980.17654970203859227No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT87770.15815235096884306No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC82480.1486203248024402No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT81310.1465121072949371No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT77160.13903424177687057No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT76730.13825942679548056No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC70220.12652908835629667No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC70200.12649305045018552No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT69840.12584436814018457No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG69630.12546597012601735No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG68800.12397039702240405No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA65830.11861876796489619No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT65450.11793404774878408No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT65150.11739347915711662No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA60890.10971740515543868No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA60810.10957325353099404No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC60660.10930296923516031No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC59620.10742899811737978No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT59590.10737494125821302No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG57500.1036089800695964No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG57250.10315850624320687No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA56880.10249180498015033No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC56200.10126651617237074No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC61300.039.0364194
GTACTAG62600.034.577131
CAACGCA91250.031.0994195
TAGGCAT77000.030.5699845
CTGTGCG83150.030.5522339
GTATCAA107650.030.0949271
TAGTACT86300.030.0527614
GCATCGT66500.030.0073768
ATCAACG97600.029.3662133
CTAACGC30250.029.2011783
CGCCTAC31000.028.7772087
TAACGCC31900.027.9845944
CTAGGCA86500.027.919214
ACTGTGC93850.027.8164228
TCTAACG31850.027.735252
GTACTGT93100.027.6857226
GTACATG216300.027.3613661
TACTGTG101850.027.3369757
ACATGGG206900.027.1605533
TACATGG216700.026.735472
Produced by FastQC (version 0.11.5)