##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146437_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5549712
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.46547172177583	31.0	30.0	34.0	27.0	34.0
2	30.58204209515737	31.0	30.0	34.0	26.0	34.0
3	30.785233540046764	31.0	31.0	34.0	27.0	34.0
4	34.24803791620178	37.0	35.0	37.0	30.0	37.0
5	34.43722791380886	37.0	35.0	37.0	30.0	37.0
6	34.62968276552009	37.0	35.0	37.0	31.0	37.0
7	34.524341443303726	37.0	35.0	37.0	30.0	37.0
8	34.46855782786566	37.0	35.0	37.0	30.0	37.0
9	35.858792852674156	39.0	35.0	39.0	30.0	39.0
10-11	35.78491433429338	39.0	35.0	39.0	30.0	39.0
12-13	35.76485266262466	39.0	35.0	39.0	30.0	39.0
14-15	36.78787007325785	40.0	36.0	41.0	29.5	41.0
16-17	36.6122650148332	40.0	36.0	41.0	28.5	41.0
18-19	36.56349824999928	40.0	36.0	41.0	28.0	41.0
20-21	36.89373070170127	40.0	36.0	41.0	30.0	41.0
22-23	36.59927812470269	40.0	36.0	41.0	28.5	41.0
24-25	36.70638395289701	40.0	36.0	41.0	29.5	41.0
26-27	36.77450901956714	40.0	36.0	41.0	30.0	41.0
28-29	36.60547051810977	40.0	35.5	41.0	29.5	41.0
30-31	36.54999700885379	40.0	35.0	41.0	29.0	41.0
32-33	36.65155768443479	40.0	35.0	41.0	30.0	41.0
34-35	36.70224923383412	40.0	35.0	41.0	30.0	41.0
36-37	36.635981650939726	40.0	35.0	41.0	30.0	41.0
38-39	36.46583345225842	39.0	35.0	41.0	29.0	41.0
40-41	36.42534549540589	39.0	35.0	41.0	29.0	41.0
42-43	36.27023267153322	39.0	35.0	41.0	28.0	41.0
44-45	36.09038505061164	39.0	35.0	41.0	28.0	41.0
46-47	35.96032658992034	39.0	34.5	41.0	28.0	41.0
48-49	35.82854578760123	38.0	34.0	40.0	28.0	41.0
50-51	35.604554614725956	38.0	34.0	40.0	27.0	41.0
52-53	35.414295462539314	38.0	34.0	40.0	27.0	41.0
54-55	35.21335179194884	38.0	34.0	40.0	27.0	41.0
56-57	35.05881224106764	37.0	33.0	40.0	27.0	41.0
58-59	34.79013442499358	37.0	33.0	40.0	26.0	41.0
60-61	34.53759303185463	36.0	33.0	40.0	26.0	41.0
62-63	34.26366863721937	36.0	33.0	39.0	26.0	41.0
64-65	33.97180961822883	35.0	32.5	39.0	26.0	41.0
66-67	33.68340203960133	35.0	32.0	38.5	26.0	40.5
68-69	33.39315238340296	35.0	32.0	38.0	25.0	40.0
70-71	33.33688721144449	35.0	32.0	37.5	25.5	40.0
72-73	33.128779024929585	35.0	32.0	37.0	25.0	40.0
74-75	32.872076965435326	35.0	32.0	36.5	25.0	39.0
76-77	32.64522474319388	35.0	32.0	36.0	25.0	39.0
78-79	32.43146482195833	35.0	32.0	36.0	24.0	39.0
80-81	32.22153203625702	35.0	31.0	35.0	24.0	37.5
82-83	31.99465981297768	35.0	31.0	35.0	24.0	37.0
84-85	31.82686056501671	35.0	31.0	35.0	24.0	37.0
86-87	31.670304242814762	35.0	31.0	35.0	24.0	36.0
88-89	31.678672785182364	35.0	31.0	35.0	24.0	36.0
90-91	31.613471924308865	35.0	31.0	35.0	24.0	36.0
92-93	31.469388771885818	35.0	31.0	35.0	23.5	36.0
94-95	31.46061074881003	35.0	31.0	35.0	24.0	35.0
96-97	31.37706569277829	35.0	31.0	35.0	23.0	35.0
98-99	31.304331648200844	35.0	31.0	35.0	23.0	35.0
100	30.821125132259116	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.699142358276351
1101	2	0.8611689982484023
1101	3	0.8505492531084506
1101	4	0.8961037447700733
1101	5	0.8009906534498725
1101	6	0.5248106316502401
1101	7	0.5367205682113223
1101	8	0.5913530107519165
1101	9	0.7533156941681511
1101	10-11	0.8010988745887673
1101	12-13	0.6395232288292121
1101	14-15	0.8687559951808268
1101	16-17	0.9707839311450712
1101	18-19	1.1126493958965682
1101	20-21	0.7974921748286121
1101	22-23	1.080174107900433
1101	24-25	0.9438517744510264
1101	26-27	0.8874894206722175
1101	28-29	1.1034050324622626
1101	30-31	1.117896828279875
1101	32-33	0.9348804807886708
1101	34-35	0.8315804809170828
1101	36-37	0.7663154638962908
1101	38-39	0.8883548765693092
1101	40-41	0.7718770892953586
1101	42-43	0.8036458736494723
1101	44-45	0.7949301090609069
1101	46-47	0.7878579226640738
1101	48-49	0.8408978593246701
1101	50-51	0.8362802728275227
1101	52-53	0.7986207191512023
1101	54-55	0.8245687710231451
1101	56-57	0.6859197828734196
1101	58-59	0.6427300669249263
1101	60-61	0.7053419390033042
1101	62-63	0.6005094925327228
1101	64-65	0.6387062611832732
1101	66-67	0.6667016145020739
1101	68-69	0.6949858500221495
1101	70-71	0.5605774187751251
1101	72-73	0.5662510463256609
1101	74-75	0.517187476337817
1101	76-77	0.5639395327164465
1101	78-79	0.5100238048959724
1101	80-81	0.5992993841178702
1101	82-83	0.5906327148659116
1101	84-85	0.5424004737895061
1101	86-87	0.538743261592117
1101	88-89	0.5183300841938028
1101	90-91	0.5737602926908565
1101	92-93	0.642475920302747
1101	94-95	0.6386526674566788
1101	96-97	0.5826025570139457
1101	98-99	0.5966813279160732
1101	100	0.6371797761543867
1102	1	0.6774462049672962
1102	2	0.8772024271850576
1102	3	0.8359685649793782
1102	4	0.9322173086255745
1102	5	0.7930175512832207
1102	6	0.5927018801866524
1102	7	0.653952153830005
1102	8	0.6363359847671504
1102	9	0.8070524950017273
1102	10-11	0.8042956777855679
1102	12-13	0.7370328160162174
1102	14-15	0.9093732274109598
1102	16-17	1.0846373895146613
1102	18-19	1.1252282149269988
1102	20-21	0.8961178502284852
1102	22-23	1.0851203444595683
1102	24-25	0.9929966081119872
1102	26-27	0.8932698767752569
1102	28-29	1.0449048229943116
1102	30-31	1.0967577523020893
1102	32-33	0.9804804721951115
1102	34-35	0.8507041529439832
1102	36-37	0.747779591166875
1102	38-39	0.8585070899473379
1102	40-41	0.8025064599247287
1102	42-43	0.8631648492974833
1102	44-45	0.8827923543425058
1102	46-47	0.867263463359933
1102	48-49	0.8242474129967974
1102	50-51	0.9129870069536139
1102	52-53	0.7988153632168178
1102	54-55	0.8983317822055099
1102	56-57	0.7887055992076242
1102	58-59	0.7925507838424153
1102	60-61	0.8204956025440637
1102	62-63	0.7844262209655852
1102	64-65	0.669484300348401
1102	66-67	0.7554303051662501
1102	68-69	0.7904024764065198
1102	70-71	0.7311010886536309
1102	72-73	0.6913751695142949
1102	74-75	0.6674935143857894
1102	76-77	0.7472691063686057
1102	78-79	0.7357918003413815
1102	80-81	0.750794770451968
1102	82-83	0.7148766429808049
1102	84-85	0.7091071004961336
1102	86-87	0.7453128210648998
1102	88-89	0.7031912445388251
1102	90-91	0.6833934552272538
1102	92-93	0.7592926519581802
1102	94-95	0.7030332331275666
1102	96-97	0.7096256414563804
1102	98-99	0.7205067498060096
1102	100	0.7595047629955047
1103	1	0.5488103541362896
1103	2	0.7038231094073986
1103	3	0.696334690621903
1103	4	0.6568121837009713
1103	5	0.6318398025161258
1103	6	0.44237064269171356
1103	7	0.5325804081884158
1103	8	0.5361119149004665
1103	9	0.5864591357441995
1103	10-11	0.7568304138298814
1103	12-13	0.6545613027884514
1103	14-15	0.7291087241455259
1103	16-17	1.0368052698206682
1103	18-19	1.0726096917021337
1103	20-21	0.7741333804083794
1103	22-23	1.0025297560893023
1103	24-25	0.895604510181812
1103	26-27	0.8062614441691807
1103	28-29	1.0051470061892402
1103	30-31	1.0454354967233712
1103	32-33	0.8492642543345355
1103	34-35	0.7743595686031455
1103	36-37	0.7485028031710357
1103	38-39	0.8244978038245421
1103	40-41	0.7792919873406916
1103	42-43	0.8121852172309829
1103	44-45	0.8608197051204982
1103	46-47	0.8259115346033781
1103	48-49	0.7432028893234417
1103	50-51	0.843091567096991
1103	52-53	0.8840415625294824
1103	54-55	0.8070472482412825
1103	56-57	0.7637636852789527
1103	58-59	0.7494069219487187
1103	60-61	0.7339627404122169
1103	62-63	0.7350011018469758
1103	64-65	0.7681922394445166
1103	66-67	0.8090223241765528
1103	68-69	0.8075220400178509
1103	70-71	0.7037806617909723
1103	72-73	0.6687155697110754
1103	74-75	0.6779021420317335
1103	76-77	0.6817995070347251
1103	78-79	0.7014366574251412
1103	80-81	0.7114818253325694
1103	82-83	0.6788518753511639
1103	84-85	0.6716280168706135
1103	86-87	0.6836269951788339
1103	88-89	0.6858935027945599
1103	90-91	0.7225557572539643
1103	92-93	0.7749535885319894
1103	94-95	0.7157132234624903
1103	96-97	0.6949504732301541
1103	98-99	0.6627709158367416
1103	100	0.7501824830802661
1104	1	0.504274380302288
1104	2	0.696915017000741
1104	3	0.6383440323232499
1104	4	0.6909912132857414
1104	5	0.6239257016260211
1104	6	0.3914724256815063
1104	7	0.38473742590559823
1104	8	0.4601427252682697
1104	9	0.5348169368932076
1104	10-11	0.5664768510076499
1104	12-13	0.4569388601623743
1104	14-15	0.747633851611063
1104	16-17	0.8510750954734831
1104	18-19	0.7660314921286826
1104	20-21	0.6418318576119262
1104	22-23	0.8845712552972458
1104	24-25	0.717134931821306
1104	26-27	0.6481226277636907
1104	28-29	0.8406261197664904
1104	30-31	0.8897832853176411
1104	32-33	0.7394098390260382
1104	34-35	0.6202639679211543
1104	36-37	0.6333777073469591
1104	38-39	0.7205034083021502
1104	40-41	0.6317121181027829
1104	42-43	0.5830136532666756
1104	44-45	0.6117627701735913
1104	46-47	0.5347252444565669
1104	48-49	0.5602982774779974
1104	50-51	0.6585310405721359
1104	52-53	0.6461635709341635
1104	54-55	0.6062984794405821
1104	56-57	0.5817582070956107
1104	58-59	0.5244628207394868
1104	60-61	0.554545445839004
1104	62-63	0.5345276341664231
1104	64-65	0.563202258110266
1104	66-67	0.6544884959869961
1104	68-69	0.5262396643820466
1104	70-71	0.45864251555987323
1104	72-73	0.453399368941227
1104	74-75	0.47782718027649196
1104	76-77	0.48305362263199925
1104	78-79	0.47401701893906534
1104	80-81	0.5173646595659207
1104	82-83	0.43965152851724554
1104	84-85	0.4395422467709835
1104	86-87	0.44480128737079383
1104	88-89	0.3718539944187391
1104	90-91	0.44153781871580833
1104	92-93	0.4296019303086993
1104	94-95	0.36841642991083035
1104	96-97	0.44486610730542964
1104	98-99	0.4710990378342821
1104	100	0.5936906524856838
1105	1	0.5035869336875329
1105	2	0.7152653387949925
1105	3	0.6543416308194061
1105	4	0.7778902270633239
1105	5	0.6179555517700877
1105	6	0.3853357602615901
1105	7	0.4409113525620896
1105	8	0.5001311131579484
1105	9	0.5596001579990073
1105	10-11	0.5801283836004387
1105	12-13	0.5508950553119973
1105	14-15	0.7299996234028328
1105	16-17	0.8599381456012409
1105	18-19	0.8812746867582248
1105	20-21	0.6814289117349333
1105	22-23	0.9105437291891576
1105	24-25	0.7780956665071841
1105	26-27	0.689375275086789
1105	28-29	0.9050435553622478
1105	30-31	0.8840469310603183
1105	32-33	0.7270664239762112
1105	34-35	0.5945149202312052
1105	36-37	0.48973202922675085
1105	38-39	0.6666816602478249
1105	40-41	0.5379147119044632
1105	42-43	0.5836296640185736
1105	44-45	0.6779579520758006
1105	46-47	0.6520788937377517
1105	48-49	0.6617816458937824
1105	50-51	0.7258520686191403
1105	52-53	0.6384564260970933
1105	54-55	0.6024452802396283
1105	56-57	0.5613197430090295
1105	58-59	0.5686125846844021
1105	60-61	0.5852481399050831
1105	62-63	0.514196475178565
1105	64-65	0.5086488623749474
1105	66-67	0.629923657602312
1105	68-69	0.5924509652490855
1105	70-71	0.5070347345583031
1105	72-73	0.5238460841624928
1105	74-75	0.5149024536230655
1105	76-77	0.5618427191912616
1105	78-79	0.5342415869851038
1105	80-81	0.5875163109831192
1105	82-83	0.5981976463079448
1105	84-85	0.5172371298973957
1105	86-87	0.5601412563110202
1105	88-89	0.507132053622751
1105	90-91	0.5386798395054413
1105	92-93	0.5862176255404314
1105	94-95	0.5636500984137278
1105	96-97	0.5490966597441123
1105	98-99	0.5807000702549878
1105	100	0.5812428979038842
1106	1	0.5474689798119314
1106	2	0.5999307275981884
1106	3	0.5922458094287144
1106	4	0.7082687008746973
1106	5	0.5492771460567383
1106	6	0.33204812611651846
1106	7	0.40407070899650677
1106	8	0.4436113376114861
1106	9	0.640240502504497
1106	10-11	0.5419418329475647
1106	12-13	0.4527678069957872
1106	14-15	0.6136777194589982
1106	16-17	0.7635681321072028
1106	18-19	0.7846640618031344
1106	20-21	0.5820680850381237
1106	22-23	0.8624273102469147
1106	24-25	0.6371003500156434
1106	26-27	0.45178606415355205
1106	28-29	0.7345695255654547
1106	30-31	0.6783940668747377
1106	32-33	0.5564415142818291
1106	34-35	0.5648100496728219
1106	36-37	0.4722587102478144
1106	38-39	0.6500226945282961
1106	40-41	0.5637398089275081
1106	42-43	0.5799178526265223
1106	44-45	0.5814124639667568
1106	46-47	0.5351622369660447
1106	48-49	0.5041227977988569
1106	50-51	0.5526670120986736
1106	52-53	0.5129084370782522
1106	54-55	0.5816345921638515
1106	56-57	0.5240613144338866
1106	58-59	0.534770532251656
1106	60-61	0.5277919400705784
1106	62-63	0.5275658476702958
1106	64-65	0.5794742886990889
1106	66-67	0.6084892817370005
1106	68-69	0.5073284823617925
1106	70-71	0.5209323293754551
1106	72-73	0.459290266749953
1106	74-75	0.4591140025962446
1106	76-77	0.45297322699107667
1106	78-79	0.41914609404604874
1106	80-81	0.49364163929206484
1106	82-83	0.4695660464787821
1106	84-85	0.4716798623123637
1106	86-87	0.47833616014222
1106	88-89	0.48321042579808093
1106	90-91	0.45478451754280513
1106	92-93	0.48215722351595147
1106	94-95	0.509202643494465
1106	96-97	0.49307767140116
1106	98-99	0.528590208505971
1106	100	0.4723308129092132
1107	1	0.45617876748475084
1107	2	0.6232930348739281
1107	3	0.5906199427286651
1107	4	0.676210010606006
1107	5	0.582154927918765
1107	6	0.3302471533544633
1107	7	0.3028033024600987
1107	8	0.40435116014189987
1107	9	0.4736588320885602
1107	10-11	0.4629456648611594
1107	12-13	0.3839323009506401
1107	14-15	0.6305625886900899
1107	16-17	0.7259785380676433
1107	18-19	0.7920852554808206
1107	20-21	0.5532897804788917
1107	22-23	0.8310035781581249
1107	24-25	0.5558340518337914
1107	26-27	0.5358703628405266
1107	28-29	0.6787656959471491
1107	30-31	0.6909913512871739
1107	32-33	0.5753657701120787
1107	34-35	0.44166305732913
1107	36-37	0.48013955572280054
1107	38-39	0.49780534956009603
1107	40-41	0.35141176704803456
1107	42-43	0.4416183249256562
1107	44-45	0.45728306703596644
1107	46-47	0.48045069102106197
1107	48-49	0.49670723248414106
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2216	90-91	-1.4500615968474548
2216	92-93	-1.3687150642565804
2216	94-95	-1.2526965434724282
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2216	98-99	-1.3416892537403307
2216	100	-1.3252586356463638
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	22620.0
1	3898.0
2	671.0
3	574.0
4	538.0
5	332.0
6	271.0
7	364.0
8	633.0
9	1083.0
10	1926.0
11	3898.0
12	6445.0
13	9612.0
14	12460.0
15	15811.0
16	19096.0
17	22144.0
18	25584.0
19	29164.0
20	33292.0
21	37598.0
22	43626.0
23	49924.0
24	56968.0
25	64288.0
26	73082.0
27	84240.0
28	99772.0
29	118190.0
30	140440.0
31	169667.0
32	208433.0
33	264366.0
34	363343.0
35	490605.0
36	728970.0
37	1124083.0
38	913716.0
39	307662.0
40	323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78333235054523	20.896368270358458	12.13017137640566	24.190128002690656
2	15.47035995357026	19.645217557508207	40.73996177673217	24.144460712189364
3	18.76311211997598	26.525106920302395	28.556434238469073	26.15534672125256
4	12.109866265207032	16.335131175886065	40.12429390190851	31.430708656998398
5	13.07475695123099	38.52158302024743	35.19324458343287	13.210415445088714
6	30.035798980332924	38.39334263178546	18.133647474470752	13.437210913410865
7	27.097852389978133	31.46620121171379	23.792279498986375	17.6436668993217
8	26.200516766476934	35.224950254842625	19.854996058238463	18.719536920441975
9	26.10201020862843	14.933198329233702	21.564948685159088	37.39984277697878
10-11	25.90687917586157	25.69271419690805	27.482419296152287	20.9179873310781
12-13	25.936346015767892	23.42198112131727	27.606828556840433	23.0348443060744
14-15	23.27254658547114	24.647544162611812	24.915589392459193	27.16431985945786
16-17	22.301770479104437	27.345040393133445	26.25559561450379	24.097593513258325
18-19	21.67981551159121	28.123395644667788	27.85209768610522	22.34469115763578
20-21	23.477320695265234	27.212149515610967	26.711973164998053	22.59855662412574
22-23	23.870363863160428	26.849332382895046	26.472537261892075	22.807766492052455
24-25	23.2961053344533	26.876382260188898	27.278500078889255	22.54901232646855
26-27	23.239888055832626	27.096782080602154	26.157952477609133	23.505377385956088
28-29	22.359556137879373	27.64297049866568	26.797154011085322	23.200319352369622
30-31	23.208207137131602	27.31336961722239	26.74349997677051	22.7349232688755
32-33	23.61737498363026	27.427000916705502	25.77117103377247	23.184453065891763
34-35	23.55133304741128	27.20089707666591	26.77694139790522	22.47082847801759
36-37	23.189648112353574	26.826740286725414	26.3825050533162	23.60110654760481
38-39	23.396499430817595	26.950802838420767	27.028596082854694	22.62410164790694
40-41	22.98188517785623	25.983556718793764	27.663703573792937	23.370854529557068
42-43	22.959935256658902	26.2635106159984	27.004709183079118	23.771844944263584
44-45	23.19342069478098	27.202117460880586	26.802584061823538	22.801877782514897
46-47	23.037425756459264	26.91647127184229	26.392841528440798	23.65326144325765
48-49	23.79128165380387	26.593207423040145	26.649531771587785	22.9659791515682
50-51	23.356206522853682	26.358251504478197	26.681149537650818	23.604392435017303
52-53	22.806813511013143	27.03171784987936	26.450197064743424	23.711271574364066
54-55	22.80059245399274	26.896536231192208	26.453753777229366	23.84911753758569
56-57	23.925011276295503	26.313702012343242	26.16949223158341	23.591794479777846
58-59	22.73709391366414	26.779406782885	27.13068856010884	23.352810743342022
60-61	22.812196018831663	27.432302205057884	26.396198525855496	23.35930325025496
62-63	23.264748011705525	26.713389262304165	25.896118179717043	24.125744546273264
64-65	22.474887457768357	26.92079925364347	26.436138510728874	24.168174777859296
66-67	23.20222037993655	26.49515657299722	26.367609538329912	23.93501350873632
68-69	22.586246523780616	26.591559702643895	26.700236714040575	24.12195705953491
70-71	22.589120431909564	26.267632443905114	26.761613968872894	24.381633155312425
72-73	23.19314850140226	26.337422428733714	27.21860683187848	23.250822237985552
74-75	23.411253725124322	26.621907807135937	26.724272864790443	23.242565602949302
76-77	23.05597259628086	26.64522075555311	26.295003152221074	24.00380349594495
78-79	22.983474206322782	26.658746722785747	26.34022763451606	24.01755143637541
80-81	22.542677757570047	26.97856511437494	27.048921825071915	23.429835302983097
82-83	23.425701022358048	25.986894601241595	28.058057706287126	22.52934667011323
84-85	23.68041874637505	26.579264188356884	26.590876708102467	23.1494403571656
86-87	22.2695229936173	27.21156319418493	27.14033037671712	23.37858343548065
88-89	23.395051212417428	25.919760076451944	26.958037976355655	23.727150734774977
90-91	23.0721883901121	26.04357608981899	27.027379824248733	23.856855695820176
92-93	23.389428807409367	26.306455402043984	26.945202862735655	23.358912927810998
94-95	22.313051973156572	26.334628596331605	27.625777094031793	23.72654233648003
96-97	22.651266379125808	26.260471937439366	27.117683762746914	23.970577920687912
98-99	22.024469193353156	27.00603290704725	26.97079168686122	23.998706212738377
100	22.887442094314085	26.315823030386937	26.12365721655588	24.673077658743097
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18900.0
1	11014.5
2	2986.0
3	2190.5
4	1095.0
5	561.0
6	433.0
7	393.0
8	364.0
9	352.0
10	349.0
11	330.0
12	328.5
13	332.0
14	352.0
15	444.0
16	521.0
17	601.0
18	757.0
19	929.0
20	1196.0
21	1597.0
22	2228.5
23	3089.5
24	4220.0
25	5947.5
26	8732.5
27	11969.5
28	16927.5
29	27454.5
30	41932.5
31	55404.0
32	73120.0
33	91115.0
34	104891.5
35	120610.0
36	136075.5
37	160872.5
38	172259.0
39	162732.5
40	151834.0
41	140499.5
42	140321.0
43	152087.5
44	168521.5
45	199371.5
46	231164.5
47	254106.5
48	268872.5
49	265532.5
50	254709.5
51	261468.0
52	265416.0
53	252425.5
54	236551.0
55	204274.0
56	178468.5
57	147706.0
58	116460.0
59	110237.0
60	91275.0
61	63390.5
62	47218.5
63	39083.0
64	28697.0
65	14571.5
66	8141.5
67	5542.0
68	4160.5
69	3062.0
70	2263.5
71	1673.5
72	1179.5
73	932.5
74	724.5
75	514.5
76	363.0
77	202.0
78	103.5
79	81.0
80	67.0
81	53.0
82	36.5
83	26.0
84	21.0
85	17.0
86	18.0
87	18.5
88	15.0
89	10.5
90	5.5
91	5.5
92	8.5
93	7.0
94	3.0
95	3.5
96	4.5
97	5.0
98	6.0
99	3.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9935650714847906
2	0.4469601305437111
3	0.48061953485153824
4	0.46613229659485034
5	0.43375223795396944
6	0.48523238683376724
7	0.503557662091294
8	0.5057739933171307
9	0.5206035916818746
10-11	0.5345953087295341
12-13	0.5328294513300871
14-15	0.5293067460077208
16-17	0.5343070054806448
18-19	0.5330366692902262
20-21	0.5308203380643897
22-23	0.4927733186875283
24-25	0.5282256088243859
26-27	0.50830565622144
28-29	0.5142248823001986
30-31	0.5173691896083977
32-33	0.5199368904188181
34-35	0.5326312428464757
36-37	0.5507312811908077
38-39	0.5397126913973194
40-41	0.5451454057435773
42-43	0.5418479373344058
44-45	0.5522628922005322
46-47	0.5503889210827516
48-49	0.5227118091893778
50-51	0.5380099003335669
52-53	0.5305680727216114
54-55	0.5398208051156529
56-57	0.5276309833735516
58-59	0.5341178064735611
60-61	0.5265498461902167
62-63	0.5207477433063193
64-65	0.5326762902291146
66-67	0.5296761345453602
68-69	0.5514790677426145
70-71	0.5345772897764784
72-73	0.5381990993406505
74-75	0.5362800808402309
76-77	0.5225766670414609
78-79	0.5537854937337289
80-81	0.5274507938429959
82-83	0.5268561683921617
84-85	0.53709994320426
86-87	0.8402327904583156
88-89	1.1045077654480089
90-91	1.0948586160867448
92-93	1.0949487108520226
94-95	1.0990480226721675
96-97	1.0926152564313247
98-99	1.0883717929867351
100	1.084416632791035
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5549712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.610983360677288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86588354503489	20.847645816680423
2	12.824626861917125	7.338503715064117
3	5.270325574542153	4.523675919557325
4	2.7124980386139175	3.104289449946103
5	1.6521068502485052	2.363420080126047
6	1.0199334552823955	1.7508779470849625
7	0.6896800678010127	1.3812697460831889
8	0.47533361828081605	1.0879809794722366
9	0.34803203844261726	0.8961784974777889
>10	1.6912333022039219	8.601475607367778
>50	0.16846903762435372	3.390776682510213
>100	0.20326447412738727	12.976121256624412
>500	0.041415086838613384	8.276350463245672
>1k	0.03474335545077787	17.70182735531405
>5k	0.0019511667010400613	3.616546511557216
>10k+	5.035268905909836E-4	2.143059971888553
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	21788	0.39259694917502025	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17893	0.3224131270235284	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	17053	0.30727720645683954	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14186	0.255616868046486	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13080	0.23568790596701236	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12705	0.2289307985711691	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11811	0.21282185453947883	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10490	0.189018817553055	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9798	0.17654970203859227	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8777	0.15815235096884306	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8248	0.1486203248024402	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8131	0.1465121072949371	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7716	0.13903424177687057	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7673	0.13825942679548056	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7022	0.12652908835629667	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7020	0.12649305045018552	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6984	0.12584436814018457	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6963	0.12546597012601735	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6880	0.12397039702240405	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6583	0.11861876796489619	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6545	0.11793404774878408	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6515	0.11739347915711662	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6089	0.10971740515543868	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6081	0.10957325353099404	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6066	0.10930296923516031	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5962	0.10742899811737978	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5959	0.10737494125821302	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5750	0.1036089800695964	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5725	0.10315850624320687	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5688	0.10249180498015033	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5620	0.10126651617237074	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.6037906111163966E-5	0.0	0.0	0.0	0.0
7	3.6037906111163966E-5	0.0	0.0	0.0	0.0
8	3.6037906111163966E-5	0.0	0.0	0.0	0.0
9	3.6037906111163966E-5	0.0	0.0	0.0	0.0
10-11	9.009476527790991E-5	0.0	0.0	0.0	0.0
12-13	1.6217057750023784E-4	0.0	0.0	9.009476527790992E-6	0.0
14-15	2.072179601391928E-4	0.0	0.0	1.8018953055581983E-5	0.0
16-17	2.792937723615208E-4	0.0	0.0	1.8018953055581983E-5	0.0
18-19	3.0632220194489374E-4	0.0	0.0	1.8018953055581983E-5	0.0
20-21	3.0632220194489374E-4	0.0	0.0	1.8018953055581983E-5	0.0
22-23	3.243411550004757E-4	0.0	0.0	3.6037906111163966E-5	0.0
24-25	4.7750225597292253E-4	0.0	0.0	1.351421479168649E-4	1.8018953055581983E-5
26-27	5.585875447230415E-4	0.0	0.0	2.8830324888931173E-4	1.8018953055581983E-5
28-29	5.585875447230415E-4	0.0	0.0	0.0012162793312517838	1.8018953055581983E-5
30-31	6.126444038897875E-4	0.0	0.0	0.006018330320564382	1.8018953055581983E-5
32-33	6.937296926399064E-4	0.0	0.0	0.016694560005996707	1.8018953055581983E-5
34-35	8.378813170845622E-4	0.0	0.0	0.03321793995796539	1.8018953055581983E-5
36-37	9.730234650014271E-4	0.0	0.0	0.06509346791328992	1.8018953055581983E-5
38-39	9.730234650014271E-4	0.0	0.0	0.11629432302072612	3.6037906111163966E-5
40-41	0.001063118230279337	0.0	0.0	0.17406308651692196	3.6037906111163966E-5
42-43	0.001063118230279337	0.0	0.0	0.23504823313353918	4.504738263895496E-5
44-45	0.001144203519029456	0.0	0.0	0.3104395327180942	5.405685916674595E-5
46-47	0.001225288807779575	0.0	0.0	0.40455252452739887	5.405685916674595E-5
48-49	0.001225288807779575	0.0	0.0	0.5196125492638177	5.405685916674595E-5
50-51	0.001225288807779575	0.0	0.0	0.6445379507981674	5.405685916674595E-5
52-53	0.0013514214791686488	0.0	0.0	0.7692471248958505	5.405685916674595E-5
54-55	0.0013694404322242308	0.0	0.0	0.9108760959127249	5.405685916674595E-5
56-57	0.0013694404322242308	0.0	0.0	1.0843805948849237	5.405685916674595E-5
58-59	0.0013694404322242308	0.0	0.0	1.2940581421162034	5.405685916674595E-5
60-61	0.0013874593852798128	0.0	0.0	1.508916138350963	5.405685916674595E-5
62-63	0.0013874593852798128	0.0	0.0	1.744838290707698	5.405685916674595E-5
64-65	0.0013874593852798128	0.0	0.0	1.9897969480218072	5.405685916674595E-5
66-67	0.0014595351975021406	0.0	0.0	2.2719287054895823	5.405685916674595E-5
68-69	0.0015676489158356326	0.0	0.0	2.5881793505681014	5.405685916674595E-5
70-71	0.0015676489158356326	0.0	0.0	2.9405760154761182	5.405685916674595E-5
72-73	0.0015766583923634237	0.0	0.0	3.3096492214370765	5.405685916674595E-5
74-75	0.0015856678688912146	0.0	0.0	3.6732356561926096	5.405685916674595E-5
76-77	0.0015856678688912146	0.0	0.0	4.089356348581692	5.405685916674595E-5
78-79	0.0016847721106969154	0.0	0.0	4.54248797054694	7.207581222232793E-5
80-81	0.0016937815872247065	0.0	0.0	5.030161925519739	8.108528875011892E-5
82-83	0.0017478384463914523	0.0	0.0	5.536746771724371	9.009476527790991E-5
84-85	0.0017928858290304074	0.0	0.0	6.0370520127891325	9.009476527790991E-5
86-87	0.0018018953055581983	0.0	0.0	6.573503273683391	9.009476527790991E-5
88	0.0018018953055581983	0.0	0.0	7.005804985916386	9.009476527790991E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6130	0.0	39.03641	94
GTACTAG	6260	0.0	34.57713	1
CAACGCA	9125	0.0	31.099419	5
TAGGCAT	7700	0.0	30.569984	5
CTGTGCG	8315	0.0	30.552233	9
GTATCAA	10765	0.0	30.094927	1
TAGTACT	8630	0.0	30.052761	4
GCATCGT	6650	0.0	30.007376	8
ATCAACG	9760	0.0	29.366213	3
CTAACGC	3025	0.0	29.201178	3
CGCCTAC	3100	0.0	28.777208	7
TAACGCC	3190	0.0	27.984594	4
CTAGGCA	8650	0.0	27.91921	4
ACTGTGC	9385	0.0	27.816422	8
TCTAACG	3185	0.0	27.73525	2
GTACTGT	9310	0.0	27.685722	6
GTACATG	21630	0.0	27.361366	1
TACTGTG	10185	0.0	27.336975	7
ACATGGG	20690	0.0	27.160553	3
TACATGG	21670	0.0	26.73547	2
>>END_MODULE