##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5549712
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84042559325601	31.0	31.0	34.0	30.0	34.0
2	31.840620378138542	31.0	31.0	34.0	30.0	34.0
3	32.02344464001015	33.0	31.0	34.0	30.0	34.0
4	35.74507848335193	37.0	35.0	37.0	35.0	37.0
5	35.64852500454078	37.0	35.0	37.0	33.0	37.0
6	35.679363721937285	37.0	35.0	37.0	33.0	37.0
7	35.600792077138415	37.0	35.0	37.0	33.0	37.0
8	35.586014553548004	37.0	35.0	37.0	33.0	37.0
9	37.19313560775766	39.0	37.0	39.0	33.0	39.0
10-11	37.137606600126276	39.0	37.0	39.0	33.0	39.0
12-13	37.063829618545974	39.0	37.0	39.0	33.0	39.0
14-15	38.26999995675451	40.0	38.0	41.0	33.0	41.0
16-17	38.273928719183985	40.0	38.0	41.0	33.0	41.0
18-19	38.31954144647506	40.0	38.0	41.0	33.0	41.0
20-21	38.33127421747291	40.0	38.0	41.0	33.0	41.0
22-23	38.295429654728025	40.0	38.0	41.0	33.0	41.0
24-25	38.227503607394404	40.0	37.0	41.0	33.0	41.0
26-27	38.091034003205934	40.0	37.0	41.0	33.0	41.0
28-29	38.0273290758151	40.0	37.0	41.0	33.0	41.0
30-31	37.90915285333726	40.0	37.0	41.0	33.0	41.0
32-33	37.805118445786015	40.0	37.0	41.0	33.0	41.0
34-35	37.715498660110654	40.0	36.5	41.0	32.0	41.0
36-37	37.63873557762997	40.0	36.0	41.0	32.0	41.0
38-39	37.53131342671476	40.0	36.0	41.0	32.0	41.0
40-41	37.40852561358139	40.0	36.0	41.0	31.0	41.0
42-43	37.29018577900979	39.5	36.0	41.0	31.0	41.0
44-45	37.09994491606051	39.0	35.5	41.0	31.0	41.0
46-47	36.88120518686375	39.0	35.0	41.0	30.5	41.0
48-49	36.637223967658144	39.0	35.0	41.0	30.0	41.0
50-51	36.4340142335314	39.0	35.0	41.0	30.0	41.0
52-53	36.240809703278295	38.5	35.0	40.5	30.0	41.0
54-55	36.03217932750384	38.0	35.0	40.0	29.0	41.0
56-57	35.772242145177984	38.0	34.5	40.0	29.0	41.0
58-59	35.4874578176309	37.0	34.0	40.0	28.0	41.0
60-61	35.22814418117552	37.0	34.0	40.0	28.0	41.0
62-63	34.94442126005818	36.0	34.0	39.5	28.0	41.0
64-65	34.6663162881245	35.5	34.0	39.0	28.0	41.0
66-67	34.443930513871706	35.0	33.0	39.0	28.0	41.0
68-69	34.25155899981837	35.0	33.0	38.5	27.5	41.0
70-71	34.048327733042726	35.0	33.0	37.5	27.0	40.0
72-73	33.84375225236913	35.0	33.0	37.0	27.0	40.0
74-75	33.66480035360394	35.0	33.0	37.0	27.0	39.5
76-77	33.50476276967165	35.0	33.0	36.0	27.0	39.0
78-79	33.39232315118334	35.0	33.0	36.0	27.0	39.0
80-81	33.24530399054942	35.0	33.0	36.0	27.0	38.0
82-83	33.055762172883924	35.0	33.0	35.0	27.0	37.0
84-85	32.919158597779486	35.0	33.0	35.0	27.0	37.0
86-87	32.78748996344315	35.0	33.0	35.0	27.0	36.0
88-89	32.67467302807785	35.0	33.0	35.0	27.0	36.0
90-91	32.584761425457756	35.0	33.0	35.0	27.0	36.0
92-93	32.50773031105037	35.0	33.0	35.0	27.0	36.0
94-95	32.46588300437932	35.0	33.0	35.0	27.0	35.0
96-97	32.41461241592357	35.0	33.0	35.0	27.0	35.0
98-99	32.37545263610076	35.0	33.0	35.0	27.0	35.0
100	31.87590148822137	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.465698348870923
1101	2	0.5632525190952862
1101	3	0.4617970326242897
1101	4	0.3285091740377055
1101	5	0.33094773306721237
1101	6	0.3743703892287229
1101	7	0.4037283306441779
1101	8	0.3847717502124084
1101	9	0.4303966087422282
1101	10-11	0.5096458979731651
1101	12-13	0.5158303950069083
1101	14-15	0.6382503910536172
1101	16-17	0.5948904005999296
1101	18-19	0.5837258505502021
1101	20-21	0.5950157712348556
1101	22-23	0.6262967255121055
1101	24-25	0.6578581802859134
1101	26-27	0.6525088360158762
1101	28-29	0.6702743128476101
1101	30-31	0.6884114395699044
1101	32-33	0.7170949501221386
1101	34-35	0.6742550736542654
1101	36-37	0.6846049070324156
1101	38-39	0.6736334926831304
1101	40-41	0.7043483070233734
1101	42-43	0.697456336094298
1101	44-45	0.6871994233517356
1101	46-47	0.6844828879251281
1101	48-49	0.6825806716552378
1101	50-51	0.6100751960494222
1101	52-53	0.6632281847802659
1101	54-55	0.6914737980687562
1101	56-57	0.6568195667015431
1101	58-59	0.6495912759233704
1101	60-61	0.6507971362993814
1101	62-63	0.6303755540574016
1101	64-65	0.5953429816961204
1101	66-67	0.578599827115589
1101	68-69	0.5919740853858926
1101	70-71	0.6140467708877893
1101	72-73	0.5702962714826256
1101	74-75	0.525067885743141
1101	76-77	0.5375051348858477
1101	78-79	0.5012641458906373
1101	80-81	0.5248868606816899
1101	82-83	0.47620110591053333
1101	84-85	0.52273698645309
1101	86-87	0.5040345799183754
1101	88-89	0.4342728600921859
1101	90-91	0.40653884595021594
1101	92-93	0.4648599308674264
1101	94-95	0.45741462979549397
1101	96-97	0.4582213869935856
1101	98-99	0.4795170182776687
1101	100	0.6063077556362728
1102	1	0.38576967539386686
1102	2	0.49258469229842206
1102	3	0.40686100091406985
1102	4	0.29567276313677837
1102	5	0.3129786054206676
1102	6	0.3494963385482208
1102	7	0.35742624563241776
1102	8	0.3500722561580716
1102	9	0.4168488661622263
1102	10-11	0.4464299741120712
1102	12-13	0.4748522333835794
1102	14-15	0.5466963536931289
1102	16-17	0.5883156849929563
1102	18-19	0.576302521191387
1102	20-21	0.6213909014809502
1102	22-23	0.5536908367771929
1102	24-25	0.5710422005667013
1102	26-27	0.595609176535568
1102	28-29	0.621757883040857
1102	30-31	0.6864297653301605
1102	32-33	0.7003411662715777
1102	34-35	0.6666658886457313
1102	36-37	0.6714406519326772
1102	38-39	0.6294265168632478
1102	40-41	0.5896902768814769
1102	42-43	0.6745837248345907
1102	44-45	0.7091278175381959
1102	46-47	0.6656700878220079
1102	48-49	0.6251555161655631
1102	50-51	0.5721434503757408
1102	52-53	0.5806881441757028
1102	54-55	0.5773655407346894
1102	56-57	0.5733443322585643
1102	58-59	0.5541114868951951
1102	60-61	0.5950204398775227
1102	62-63	0.5874956382742553
1102	64-65	0.5601824433097704
1102	66-67	0.5893179262207866
1102	68-69	0.5609648795379769
1102	70-71	0.48989522351043746
1102	72-73	0.5050952359590042
1102	74-75	0.40879727463382665
1102	76-77	0.44055928503676967
1102	78-79	0.4081187966162503
1102	80-81	0.453436590521747
1102	82-83	0.4768615207644942
1102	84-85	0.4142774889613321
1102	86-87	0.4751948175302232
1102	88-89	0.4489300308783868
1102	90-91	0.4427441460264845
1102	92-93	0.45972850315535396
1102	94-95	0.4181145750115647
1102	96-97	0.4640718805860189
1102	98-99	0.46720803693643376
1102	100	0.5180106763714534
1103	1	0.2836897716274436
1103	2	-0.16555714444868386
1103	3	0.19086606291136832
1103	4	0.1913694744030252
1103	5	0.22124207264545248
1103	6	0.2646880669108782
1103	7	0.27205406193336756
1103	8	0.3036175142730002
1103	9	0.3310292380591022
1103	10-11	0.3550493456386832
1103	12-13	0.3895277145079987
1103	14-15	0.49681196957236295
1103	16-17	0.5172537954703458
1103	18-19	0.521798682104496
1103	20-21	0.5312917649061077
1103	22-23	0.4952835233036197
1103	24-25	0.47698542345277417
1103	26-27	0.4911285440640327
1103	28-29	0.5367731020608986
1103	30-31	0.5839198351586106
1103	32-33	0.584380125572217
1103	34-35	0.5702692589068477
1103	36-37	0.5334323879439609
1103	38-39	0.5920995712884078
1103	40-41	0.5772700268062962
1103	42-43	0.587971456096767
1103	44-45	0.561162924714516
1103	46-47	0.6065579885706995
1103	48-49	0.5899642397669496
1103	50-51	0.6005016214510874
1103	52-53	0.5671773743940207
1103	54-55	0.5115889882816589
1103	56-57	0.5558090660256667
1103	58-59	0.4825817450535794
1103	60-61	0.5043640664022533
1103	62-63	0.5739180821133871
1103	64-65	0.5581837192390395
1103	66-67	0.5515649871507549
1103	68-69	0.5287102221354161
1103	70-71	0.5293038111749482
1103	72-73	0.530061625479
1103	74-75	0.5077280667703405
1103	76-77	0.4497562666122832
1103	78-79	0.4844998857453007
1103	80-81	0.4579311643457302
1103	82-83	0.41675478771705343
1103	84-85	0.3979827597312493
1103	86-87	0.45249826097474966
1103	88-89	0.40933898880575725
1103	90-91	0.4725651856480937
1103	92-93	0.47572646584050204
1103	94-95	0.42827514392999433
1103	96-97	0.43657289315930115
1103	98-99	0.4574491249877184
1103	100	0.4554932018770046
1104	1	0.28706026670773355
1104	2	0.4295102498358858
1104	3	0.33755725510882684
1104	4	0.2606703949252349
1104	5	0.2567784260412367
1104	6	0.28371212460969986
1104	7	0.29088487583198486
1104	8	0.21124494076939726
1104	9	0.27070804323390263
1104	10-11	0.31237193445377187
1104	12-13	0.35813914305227
1104	14-15	0.46836344025545884
1104	16-17	0.46099411545655045
1104	18-19	0.43314886961073285
1104	20-21	0.44431908588569513
1104	22-23	0.43571510338742314
1104	24-25	0.40658455460258125
1104	26-27	0.4669758529438113
1104	28-29	0.48712132793207275
1104	30-31	0.5257324362025031
1104	32-33	0.48627973385630696
1104	34-35	0.47700271215182966
1104	36-37	0.526156752076119
1104	38-39	0.4568286055922428
1104	40-41	0.4520255014248775
1104	42-43	0.45221547969614306
1104	44-45	0.40375960263223476
1104	46-47	0.4067610439412306
1104	48-49	0.43040481661938657
1104	50-51	0.4866894087959963
1104	52-53	0.4844045567215147
1104	54-55	0.4446102525686939
1104	56-57	0.40808283803465173
1104	58-59	0.41657163879664694
1104	60-61	0.441365399282013
1104	62-63	0.369006355691198
1104	64-65	0.36079481914661926
1104	66-67	0.3785236515821424
1104	68-69	0.41005988681057204
1104	70-71	0.3943156835145274
1104	72-73	0.40407861816819235
1104	74-75	0.3721787783977675
1104	76-77	0.35614641674627734
1104	78-79	0.3162946018921602
1104	80-81	0.32870144391097966
1104	82-83	0.30813625681808077
1104	84-85	0.29328848350940717
1104	86-87	0.3291706584132825
1104	88-89	0.32346527356128263
1104	90-91	0.30907570236136905
1104	92-93	0.2577865696869779
1104	94-95	0.2664162146201505
1104	96-97	0.30588562830597965
1104	98-99	0.27135385158440783
1104	100	0.28885661497263015
1105	1	0.13954010589735688
1105	2	-0.4037734556458119
1105	3	0.02614878036637691
1105	4	0.09036261751462149
1105	5	0.1540906542228413
1105	6	0.17740801562532482
1105	7	0.21004138795429128
1105	8	0.232492036228777
1105	9	0.30976746229753616
1105	10-11	0.31490170410387464
1105	12-13	0.30647435054309113
1105	14-15	0.35093337215232623
1105	16-17	0.3866585335377124
1105	18-19	0.39675192426552286
1105	20-21	0.36869382538991147
1105	22-23	0.3655920989414909
1105	24-25	0.4314443357477842
1105	26-27	0.40606101393374416
1105	28-29	0.39624450062852645
1105	30-31	0.44906610038817973
1105	32-33	0.4519163805144828
1105	34-35	0.4614516836246878
1105	36-37	0.4366242746820035
1105	38-39	0.4816556242857999
1105	40-41	0.47023621966900464
1105	42-43	0.4880527398836776
1105	44-45	0.46403803505383223
1105	46-47	0.47851252783416953
1105	48-49	0.4758297137424634
1105	50-51	0.4444806183719905
1105	52-53	0.44339970569988907
1105	54-55	0.38128632431845944
1105	56-57	0.5217295490597422
1105	58-59	0.5175325832913273
1105	60-61	0.5124385551735813
1105	62-63	0.517495490217776
1105	64-65	0.5077436947933478
1105	66-67	0.4829847516177779
1105	68-69	0.49727716440416003
1105	70-71	0.45447755100655485
1105	72-73	0.43141218632599276
1105	74-75	0.3562211847917709
1105	76-77	0.36114244853554567
1105	78-79	0.37394295991636284
1105	80-81	0.34496372428252187
1105	82-83	0.3151626931308158
1105	84-85	0.37115906133495713
1105	86-87	0.3303830506895764
1105	88-89	0.35940632858485344
1105	90-91	0.34784808684246116
1105	92-93	0.30279080015872495
1105	94-95	0.25393396262929
1105	96-97	0.24435362863037824
1105	98-99	0.248804932846177
1105	100	0.3486152460831633
1106	1	0.25590950034249715
1106	2	0.2902281622167351
1106	3	0.20661287113657067
1106	4	0.17607223457473964
1106	5	0.19959157163367536
1106	6	0.1521200536922649
1106	7	0.19227905118165012
1106	8	0.16482278375872994
1106	9	0.2522399138912732
1106	10-11	0.25447364479378365
1106	12-13	0.2667834937971918
1106	14-15	0.3221538164494575
1106	16-17	0.3021620678370951
1106	18-19	0.36986898229878307
1106	20-21	0.3252533825267818
1106	22-23	0.2813200052865028
1106	24-25	0.2157270822469073
1106	26-27	0.3178639524569533
1106	28-29	0.28451351326755514
1106	30-31	0.2896339439671607
1106	32-33	0.32258118992825047
1106	34-35	0.27953911265539233
1106	36-37	0.3386826016715503
1106	38-39	0.30418440402068825
1106	40-41	0.2646387831898096
1106	42-43	0.28241766140846636
1106	44-45	0.3141318652944278
1106	46-47	0.2780121242179945
1106	48-49	0.24804047568468235
1106	50-51	0.31102533479356964
1106	52-53	0.2747968852737941
1106	54-55	0.2780374019665999
1106	56-57	0.2973170011189552
1106	58-59	0.2995493904690818
1106	60-61	0.20689758434297545
1106	62-63	0.24113704088644283
1106	64-65	0.21930497860414988
1106	66-67	0.22429880794872759
1106	68-69	0.27171655549470586
1106	70-71	0.2136742432042098
1106	72-73	0.25526182715153567
1106	74-75	0.26483770048032085
1106	76-77	0.21229532491894076
1106	78-79	0.20601852497567563
1106	80-81	0.18708247517031396
1106	82-83	0.16779727846402182
1106	84-85	0.18058326329610708
1106	86-87	0.2262919749511454
1106	88-89	0.25237865951449123
1106	90-91	0.1973400831533283
1106	92-93	0.2928795272277327
1106	94-95	0.28654712202155963
1106	96-97	0.21601842368284707
1106	98-99	0.2465672847499505
1106	100	0.29816303908754094
1107	1	0.10685216132033304
1107	2	-0.43783886976407516
1107	3	-0.024075776068876564
1107	4	0.06915346049861881
1107	5	0.12604604337258252
1107	6	0.16452044232975993
1107	7	0.21551268800545387
1107	8	0.16657538232416158
1107	9	0.19009589815676975
1107	10-11	0.233846133888008
1107	12-13	0.1688262108880636
1107	14-15	0.22206441788405584
1107	16-17	0.2886286335128787
1107	18-19	0.24411148929339532
1107	20-21	0.21166558635825083
1107	22-23	0.22212291252532168
1107	24-25	0.19427035386400604
1107	26-27	0.2864279943489336
1107	28-29	0.2753368260685036
1107	30-31	0.3215338898444031
1107	32-33	0.3271150010878401
1107	34-35	0.3298601736071163
1107	36-37	0.33877946637998946
1107	38-39	0.4170302901887979
1107	40-41	0.3652079625429465
1107	42-43	0.3241340926691265
1107	44-45	0.3454801152845164
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2216	74-75	-0.8914895503191644
2216	76-77	-0.8074190082378436
2216	78-79	-0.7498769360831545
2216	80-81	-0.6644915278714905
2216	82-83	-0.6014089559455869
2216	84-85	-0.6479993789569392
2216	86-87	-0.5463914881371039
2216	88-89	-0.5906619450376667
2216	90-91	-0.6120976813134646
2216	92-93	-0.6268704193025343
2216	94-95	-0.6114377481341577
2216	96-97	-0.6891094017475545
2216	98-99	-0.6716004314144186
2216	100	-0.6549993293767358
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	9.0
13	69.0
14	274.0
15	828.0
16	2108.0
17	4167.0
18	7671.0
19	11926.0
20	17215.0
21	24539.0
22	33627.0
23	40048.0
24	43268.0
25	46404.0
26	53782.0
27	67210.0
28	85140.0
29	106020.0
30	131693.0
31	162994.0
32	202896.0
33	257344.0
34	351371.0
35	483081.0
36	729182.0
37	1242533.0
38	1054642.0
39	388820.0
40	850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40878820022253	21.40364023264791	13.325641837986254	24.861929729143306
2	15.06915124616402	20.188794164445717	39.82659480343026	24.91545978596
3	18.218967527330847	26.236172401925188	29.476195538769527	26.068664531974434
4	12.303525965979498	16.844375352033346	38.86374225526642	31.98835642672074
5	12.720625519635604	38.65097822134432	35.189930304409614	13.438465954610471
6	29.963320619160054	38.754659701260174	17.626356106406963	13.655663573172806
7	26.355609692811115	32.02287339089821	23.78929956321806	17.83221735307261
8	26.337114430442515	35.12652548456568	19.51594965648668	19.02041042850512
9	26.019980856664276	14.474264610487896	21.462068662301757	38.04368587054607
10-11	25.87260383962267	25.832565725933165	27.231800497034804	21.063029937409365
12-13	26.011016427519124	23.466758275024002	27.857923077810163	22.66430221964671
14-15	23.223306471530023	24.520978723891197	25.45348158398531	26.802233220593468
16-17	21.945093119553004	27.119314605060097	26.748204069169812	24.18738820621709
18-19	21.346927552276586	27.327355005088556	29.179361019094323	22.146356423540535
20-21	22.91362690490861	26.530961040566364	28.34623656359609	22.20917549092894
22-23	23.253643281003473	26.08285756913747	28.135809205163266	22.52768994469579
24-25	22.76942479177298	26.12024732094206	28.936916005731465	22.17341188155349
26-27	22.736378049916564	26.3586182390727	27.799704885001912	23.105298826008823
28-29	21.91815539256812	26.946632545977163	28.438908181181294	22.696303880273426
30-31	22.854816610303384	26.575784473140228	28.239843797299752	22.32955511925664
32-33	23.261315181760782	26.719710860671693	27.442928569987057	22.576045387580475
34-35	23.099532117672965	26.546673091054164	28.255076052951466	22.09871873832141
36-37	22.8181570503118	26.131608270843603	27.915214339050387	23.13502033979421
38-39	22.973074999207167	26.186331831273407	28.616620105691972	22.223973063827458
40-41	22.514506658296234	25.36289845222069	29.12580029201615	22.996794597466923
42-43	22.715521423824043	25.58098677679204	28.256197728353253	23.44729407103065
44-45	22.91148621766319	26.49478026967886	27.97010007005769	22.623633442600262
46-47	22.908981583188464	26.366476314446587	27.281776063334455	23.442766039030495
48-49	23.81162216896196	26.198828870889475	27.272745291761503	22.716803668387065
50-51	23.14445534691308	26.104023605551387	27.149987886757714	23.60153316077782
52-53	22.548539455741125	26.685375745624277	26.98585980677916	23.780224991855434
54-55	22.6351677641442	26.591438522037965	26.826217147750075	23.947176566067764
56-57	23.731511976081624	26.00219020551984	26.60823115452481	23.65806666387373
58-59	22.49731201831882	26.49959826679013	27.550447294206553	23.452642420684494
60-61	22.64345259246072	27.124427086974666	26.81587031245608	23.416250008108534
62-63	23.16086852795244	26.3428804954203	26.29335540294704	24.20289557368022
64-65	22.339429505531097	26.535304895100865	26.80580541837126	24.31946018099678
66-67	22.922180466301675	26.17878188994312	26.81381484300446	24.085222800750742
68-69	22.462426142685203	26.04937278324445	27.204781790663223	24.28341928340712
70-71	22.408072044618134	25.766405654244075	27.267161014200482	24.55836128693732
72-73	23.05431344905826	25.746354045038732	27.62693811859066	23.57239438731235
74-75	23.130370838730393	26.196210302045863	27.11354759763292	23.55987126159082
76-77	22.828282882887265	26.19551713565476	26.59318869649807	24.383011284959903
78-79	22.590281282732725	26.280861447401588	26.781493645673095	24.34736362419259
80-81	22.199605733087786	26.719719111854317	27.415497500693753	23.665177654364143
82-83	23.073186852353956	25.667068556262528	28.39979767973177	22.85994691165174
84-85	23.400988536985803	26.229152524780545	26.840045965221908	23.52981297301174
86-87	21.95057275816285	26.857877983626004	27.383107526587196	23.80844173162395
88-89	22.91182540508469	25.704464660198283	27.1764514549351	24.207258479781917
90-91	22.487609417707503	25.71504435814045	27.384655500005767	24.412690724146284
92-93	22.861419631726278	26.047197076645784	27.070733264110665	24.02065002751727
94-95	21.72595759372275	26.009065655139135	27.76833720251264	24.496639548625478
96-97	22.072339447094556	25.92189349283028	27.278415799193002	24.727351260882163
98-99	21.42089391947715	26.671979357089377	27.18052906468118	24.72659765875229
100	22.29023029911311	25.937509073337456	26.351532787637645	25.420727839911795
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	110.0
1	75.0
2	73.0
3	108.0
4	73.0
5	55.5
6	132.0
7	333.5
8	566.0
9	629.0
10	528.5
11	437.0
12	445.5
13	518.5
14	679.0
15	1001.0
16	1380.5
17	1835.5
18	2454.0
19	3253.5
20	4240.5
21	5314.0
22	6534.5
23	7754.5
24	9078.0
25	10729.5
26	13357.5
27	16325.0
28	20745.5
29	30283.5
30	44287.5
31	56827.0
32	73590.5
33	91636.5
34	103863.5
35	118769.5
36	133854.0
37	158273.5
38	169988.5
39	160289.5
40	149389.0
41	138399.5
42	137715.0
43	148315.0
44	164870.0
45	197894.0
46	230010.0
47	253780.5
48	271376.0
49	267301.5
50	254646.5
51	259203.5
52	262449.0
53	251098.5
54	239990.5
55	207525.5
56	179113.5
57	148773.5
58	116612.5
59	113121.5
60	93942.0
61	62798.0
62	46004.5
63	38142.5
64	27844.0
65	13472.0
66	7175.0
67	4919.0
68	3810.0
69	2771.0
70	1981.5
71	1500.5
72	1046.5
73	790.5
74	592.0
75	409.0
76	269.5
77	138.5
78	61.5
79	30.0
80	13.5
81	13.0
82	9.5
83	4.0
84	2.5
85	1.0
86	2.5
87	3.5
88	2.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01308175991835252
2	0.005081344761674119
3	0.0021983122727810022
4	0.028596078499208605
5	0.0027028429583372975
6	0.0
7	5.766064977786235E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009126599722652274
16-17	0.0027478903409762527
18-19	0.0
20-21	6.306633569453694E-5
22-23	7.928339344456073E-4
24-25	0.0
26-27	1.9820848361140183E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.684927794451316E-4
36-37	0.0
38-39	0.0
40-41	3.423601080560577E-4
42-43	6.757107395843244E-4
44-45	0.0
46-47	0.0
48-49	4.5047382638954957E-4
50-51	9.009476527790992E-6
52-53	0.0
54-55	2.8830324888931173E-4
56-57	8.108528875011892E-5
58-59	1.6217057750023784E-4
60-61	7.207581222232793E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013604309556964397
70-71	7.207581222232794E-4
72-73	0.0
74-75	1.5316110097244687E-4
76-77	0.0028379851062541625
78-79	5.225496386118774E-4
80-81	0.003099259925560101
82-83	0.0017118005402802885
84-85	0.005387666963619013
86-87	0.0076400360955667605
88-89	0.0073787612762608226
90-91	0.0060543682266755465
92-93	0.008468907936123532
94-95	0.0015406204862522597
96-97	0.004757003606673644
98-99	0.0019280279769472723
100	0.08322954416373318
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5549712.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.201452284413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.73199919853843	14.371443914810117
2	16.1975286802517	7.192173202400383
3	7.047635293042828	4.6940321602930615
4	3.621207460301721	3.215842585673962
5	2.1575788078445357	2.395069147611057
6	1.337508204828084	1.7816774753700957
7	0.9178441258617701	1.4264230795394195
8	0.6425827346455015	1.1413015937615778
9	0.48183180491494254	0.9627629243338521
>10	2.2469603137741307	8.925248596229974
>50	0.22268990036833128	3.496031231625454
>100	0.2796069779739086	13.719251986065927
>500	0.059376354075497594	9.127139423587591
>1k	0.051680919364444404	20.593950319397734
>5k	0.0034021921838662456	4.946480140448085
>10k+	5.670320306443743E-4	2.0111722188517898
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	24637	0.4439329464303733	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17702	0.3189715069899123	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	15736	0.2835462452826381	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	14244	0.25666196732370977	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	14000	0.25226534277814777	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	13804	0.2487336279792537	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	11707	0.2109478834216983	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9908	0.17853178687470628	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	8771	0.1580442372505096	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8704	0.1568369673957856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8110	0.14613370928076988	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8059	0.1452147426749352	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7971	0.143629074806044	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7925	0.1428002029654872	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7872	0.14184519845354138	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7829	0.14107038347215134	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7817	0.14085415603548437	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7542	0.1358989439451993	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7458	0.13438535188853043	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7144	0.1287274006290777	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7054	0.1271056948540753	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7041	0.12687144846435272	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6896	0.12425870027129335	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6860	0.12361001796129241	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6667	0.12013236002156509	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6539	0.11782593403045058	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6515	0.11739347915711662	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6222	0.1121139259118311	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6133	0.1105102390898843	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	6129	0.11043816327766197	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6005	0.10820381309876981	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5965	0.10748305497654653	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5804	0.10458200353459783	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5772	0.1040053970368192	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5761	0.10380718855320781	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5755	0.10369907483487432	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5693	0.10258189974542824	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.8018953055581983E-5	0.0	0.0	0.0	0.0
7	1.8018953055581983E-5	0.0	0.0	0.0	0.0
8	1.8018953055581983E-5	0.0	0.0	0.0	0.0
9	1.8018953055581983E-5	0.0	0.0	0.0	0.0
10-11	1.4415162444465586E-4	0.0	0.0	0.0	0.0
12-13	2.5226534277814777E-4	0.0	0.0	9.009476527790992E-6	0.0
14-15	2.8830324888931173E-4	0.0	0.0	1.8018953055581983E-5	0.0
16-17	3.333506315282667E-4	0.0	0.0	1.8018953055581983E-5	0.0
18-19	3.513695845838487E-4	0.0	0.0	1.8018953055581983E-5	0.0
20-21	3.6037906111163965E-4	0.0	0.0	1.8018953055581983E-5	1.8018953055581983E-5
22-23	4.054264437505946E-4	0.0	0.0	3.6037906111163966E-5	1.8018953055581983E-5
24-25	6.036349273619965E-4	0.0	0.0	1.351421479168649E-4	1.8018953055581983E-5
26-27	7.117486456954883E-4	0.0	0.0	2.8830324888931173E-4	1.8018953055581983E-5
28-29	7.387770752788613E-4	0.0	0.0	0.0012883551434741119	1.8018953055581983E-5
30-31	8.108528875011892E-4	0.0	0.0	0.006153472468481247	1.8018953055581983E-5
32-33	8.108528875011892E-4	0.0	0.0	0.01686574006002474	1.8018953055581983E-5
34-35	8.919381762513081E-4	0.0	0.0	0.03344317687116016	1.8018953055581983E-5
36-37	9.730234650014271E-4	0.0	0.0	0.06588630184773553	1.8018953055581983E-5
38-39	9.730234650014271E-4	0.0	0.0	0.11743852653975557	1.8018953055581983E-5
40-41	0.001081137183334919	0.0	0.0	0.175828943916369	1.8018953055581983E-5
42-43	0.001081137183334919	0.0	0.0	0.23767900027965416	1.8018953055581983E-5
44-45	0.00118024142514062	0.0	0.0	0.3145838919208781	1.8018953055581983E-5
46-47	0.0012793456669463207	0.0	0.0	0.41062491170713	2.7028429583372976E-5
48-49	0.0012793456669463207	0.0	0.0	0.5275949454674405	3.6037906111163966E-5
50-51	0.0012793456669463207	0.0	0.0	0.6541060148706817	3.6037906111163966E-5
52-53	0.0013334025261130668	0.0	0.0	0.7806260937504504	3.6037906111163966E-5
54-55	0.0013514214791686488	0.0	0.0	0.9236875715352437	3.6037906111163966E-5
56-57	0.0013514214791686488	0.0	0.0	1.0989669373834174	3.6037906111163966E-5
58-59	0.0013784499087520219	0.0	0.0	1.3102932188192828	3.6037906111163966E-5
60-61	0.0014054783383353948	0.0	0.0	1.5274396220921012	3.6037906111163966E-5
62-63	0.0014054783383353948	0.0	0.0	1.7645600348270325	7.207581222232793E-5
64-65	0.0014054783383353948	0.0	0.0	2.0119152128975344	7.207581222232793E-5
66-67	0.0014054783383353948	0.0	0.0	2.296893964948091	7.207581222232793E-5
68-69	0.0014415162444465586	0.0	0.0	2.6161537751868926	7.207581222232793E-5
70-71	0.0014415162444465586	0.0	0.0	2.972325410760054	7.207581222232793E-5
72-73	0.0014415162444465586	0.0	0.0	3.3458042507431016	7.207581222232793E-5
74-75	0.0014415162444465586	0.0	0.0	3.714949532516282	7.207581222232793E-5
76-77	0.0014415162444465586	0.0	0.0	4.138971535820238	7.207581222232793E-5
78-79	0.0015135920566688866	0.0	0.0	4.601265795414249	7.207581222232793E-5
80-81	0.0015135920566688866	0.0	0.0	5.0987420608492835	7.207581222232793E-5
82-83	0.0015586394393078415	0.0	0.0	5.615768890349625	7.207581222232793E-5
84-85	0.0016126962984745873	0.0	0.0	6.125425968050234	8.108528875011892E-5
86-87	0.0016217057750023784	0.0	0.0	6.671724586789368	9.91042418057009E-5
88	0.0016397247280579604	0.0	0.0	7.112477188005432	1.081137183334919E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10150	0.0	37.571213	1
GTATCAA	16420	0.0	35.481216	1
GGGCACC	5600	0.0	35.277573	94
GTACTAG	6085	0.0	32.069183	1
CAACGCA	17795	0.0	31.005836	5
ATCAACG	18310	0.0	30.245682	3
TAGGCAT	7830	0.0	29.23079	5
TATCAAC	20980	0.0	27.720558	2
GTGGTAT	2765	0.0	27.549913	1
AACGCAG	21055	0.0	27.45437	6
CTAGGCA	8665	0.0	27.180904	4
CTGTGCG	8385	0.0	26.459332	9
TAGTACT	8310	0.0	26.248934	4
TATTCTC	3320	0.0	26.188282	5
ACTGTGC	9180	0.0	25.596992	8
ACGCAGA	21935	0.0	25.13171	7
CGCAGAG	22070	0.0	24.93539	8
AGGGCTA	6350	0.0	24.793879	5
GTACTGT	9245	0.0	24.603682	6
TCAACGC	23025	0.0	24.48016	4
>>END_MODULE