FastQCFastQC Report
Mon 16 Jan 2017
ERR1146436_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146436_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493664
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136170.3030266615394476No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC121470.2703139353543122No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT104570.23270542701902056No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA95260.2119873671017682No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC84230.18744169568530267No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA82750.18414816951156115No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC76380.16997265483133586No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC66840.14874276314383986No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG55820.12421934528260235No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT52040.11580750140642469No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT48850.10870861728869805No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC46920.10441368112969727No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC46590.10367931380717384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG39400.034.6014941
GTATCAA83200.033.395291
TAGGCAT46450.032.6664965
CAACGCA79050.031.7340225
GCATCGT40450.031.3399058
GGTATCA52600.030.9404491
ATCAACG83900.029.961943
GGGCACC41150.029.37074994
CTAGGCA53950.028.9115434
TAGTACT48350.027.4016864
CTGTGCG46100.027.2964239
TATCAAC100950.027.2299692
CTAACGC18500.027.1762923
GTACATG173200.026.582461
AACGCAG98550.026.3428426
TAACGCC19250.025.8701974
TACATGG179150.025.3633862
ACATGGG173750.025.2580743
ACGCAGA98000.025.1521997
TCTAACG19100.025.0936832
Produced by FastQC (version 0.11.5)