##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146436_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4493664 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.445169020202666 31.0 30.0 34.0 27.0 34.0 2 30.570467440378273 31.0 30.0 34.0 26.0 34.0 3 30.7438261961731 31.0 31.0 34.0 27.0 34.0 4 34.25670010040804 37.0 35.0 37.0 30.0 37.0 5 34.44509402572155 37.0 35.0 37.0 30.0 37.0 6 34.59701504162305 37.0 35.0 37.0 30.0 37.0 7 34.4705220506028 37.0 35.0 37.0 30.0 37.0 8 34.392520891637645 37.0 35.0 37.0 30.0 37.0 9 35.79066503414585 39.0 35.0 39.0 30.0 39.0 10-11 35.71592146186275 39.0 35.0 39.0 29.5 39.0 12-13 35.70646548117527 39.0 35.0 39.0 30.0 39.0 14-15 36.73703196322644 40.0 36.0 41.0 29.5 41.0 16-17 36.56902118182401 40.0 36.0 41.0 28.0 41.0 18-19 36.52683834394383 40.0 36.0 41.0 27.5 41.0 20-21 36.86132029453026 40.0 36.0 41.0 30.0 41.0 22-23 36.58656243546469 40.0 36.0 41.0 28.0 41.0 24-25 36.709441004044805 40.0 36.0 41.0 29.5 41.0 26-27 36.792765213420495 40.0 36.0 41.0 30.0 41.0 28-29 36.62953950273096 40.0 36.0 41.0 29.5 41.0 30-31 36.57380858916021 40.0 35.0 41.0 29.0 41.0 32-33 36.67783806265889 40.0 35.5 41.0 30.0 41.0 34-35 36.72814433833949 40.0 35.0 41.0 30.0 41.0 36-37 36.66039105282459 40.0 35.0 41.0 30.0 41.0 38-39 36.50254224614925 39.0 35.0 41.0 29.0 41.0 40-41 36.47256548331161 39.0 35.0 41.0 29.0 41.0 42-43 36.317444294900554 39.0 35.0 41.0 28.5 41.0 44-45 36.15439193940624 39.0 35.0 41.0 28.0 41.0 46-47 36.03807972736725 39.0 35.0 41.0 28.0 41.0 48-49 35.92601471761129 39.0 34.5 41.0 28.0 41.0 50-51 35.72989580440371 38.5 34.0 40.0 27.5 41.0 52-53 35.568521478241365 38.0 34.0 40.0 27.0 41.0 54-55 35.38254050592123 38.0 34.0 40.0 27.0 41.0 56-57 35.261000377420295 38.0 34.0 40.0 27.0 41.0 58-59 35.02875960463444 37.0 33.0 40.0 26.5 41.0 60-61 34.78627596544824 37.0 33.0 40.0 26.0 41.0 62-63 34.531199373161854 36.0 33.0 40.0 26.0 41.0 64-65 34.25216682867255 36.0 33.0 39.0 26.0 41.0 66-67 33.96118846446908 35.0 32.0 39.0 26.0 41.0 68-69 33.669732316434875 35.0 32.0 39.0 26.0 40.5 70-71 33.57613352934265 35.0 32.0 38.0 26.0 40.0 72-73 33.351454737158804 35.0 32.0 37.5 26.0 40.0 74-75 33.07384875682739 35.0 32.0 37.0 25.0 39.5 76-77 32.82484872033156 35.0 32.0 36.5 25.0 39.0 78-79 32.600065225170376 35.0 32.0 36.0 25.0 39.0 80-81 32.35439354166222 35.0 31.5 36.0 24.5 38.0 82-83 32.12031529282118 35.0 31.0 35.0 24.0 37.0 84-85 31.92118747641123 35.0 31.0 35.0 24.0 37.0 86-87 31.73970806451039 35.0 31.0 35.0 24.0 36.0 88-89 31.71472310791372 35.0 31.0 35.0 24.0 36.0 90-91 31.634292750859878 35.0 31.0 35.0 24.0 36.0 92-93 31.495783730158728 35.0 31.0 35.0 24.0 36.0 94-95 31.461895571186453 35.0 31.0 35.0 24.0 35.5 96-97 31.375990060672095 35.0 31.0 35.0 23.0 35.0 98-99 31.29393319126664 35.0 31.0 35.0 23.0 35.0 100 30.81348672263881 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7866225845033838 1101 2 0.9139169463522485 1101 3 0.8921785830783762 1101 4 0.9377993244304008 1101 5 0.7960558752056386 1101 6 0.6398372237928314 1101 7 0.6744470271200527 1101 8 0.7296411340734608 1101 9 0.9028502925344881 1101 10-11 0.9303280290727969 1101 12-13 0.7817744422872295 1101 14-15 0.9477062103319014 1101 16-17 1.142723551351068 1101 18-19 1.2132111138542925 1101 20-21 0.9227155176997357 1101 22-23 1.1928471991758443 1101 24-25 1.0735011451021848 1101 26-27 0.9770175739427316 1101 28-29 1.1495333169869042 1101 30-31 1.1531065763204396 1101 32-33 0.9680751677023807 1101 34-35 0.7873195414094951 1101 36-37 0.8026711490710738 1101 38-39 0.9302346647475375 1101 40-41 0.8423442768011427 1101 42-43 0.8741573515763363 1101 44-45 0.9210179737672419 1101 46-47 0.8840199136743863 1101 48-49 0.8137050234826191 1101 50-51 0.8435470280905548 1101 52-53 0.9060995606403139 1101 54-55 0.8879108462748491 1101 56-57 0.752401735214498 1101 58-59 0.7586510379678515 1101 60-61 0.8069425562353558 1101 62-63 0.6626200768038757 1101 64-65 0.72262169726379 1101 66-67 0.7210054959295391 1101 68-69 0.8241882664018831 1101 70-71 0.6810187386249211 1101 72-73 0.6863638119590334 1101 74-75 0.6515483863916529 1101 76-77 0.5931656841358546 1101 78-79 0.5845738380269694 1101 80-81 0.6629925974886746 1101 82-83 0.6733868216246179 1101 84-85 0.6723432885023328 1101 86-87 0.645082908278134 1101 88-89 0.6286918264640882 1101 90-91 0.6152864981441866 1101 92-93 0.6840248092160941 1101 94-95 0.6751263454449514 1101 96-97 0.6167588668260784 1101 98-99 0.6614779813417222 1101 100 0.6718194367678265 1102 1 0.6027984800341315 1102 2 0.7469020585207033 1102 3 0.7041160161406808 1102 4 0.8150287168410344 1102 5 0.6860198227674701 1102 6 0.4967345044830793 1102 7 0.5431820344422107 1102 8 0.5718280022635795 1102 9 0.7335443254487117 1102 10-11 0.7326535633107483 1102 12-13 0.7423708716734296 1102 14-15 0.857797657128728 1102 16-17 1.0690427575861179 1102 18-19 1.1040165425355966 1102 20-21 0.8961443473897432 1102 22-23 1.084351439473977 1102 24-25 0.9587128150747617 1102 26-27 0.853938147926641 1102 28-29 1.0433489142214256 1102 30-31 1.0709561575841704 1102 32-33 0.910075895308303 1102 34-35 0.8262399695648597 1102 36-37 0.7761430314820359 1102 38-39 0.9003600679105475 1102 40-41 0.8353549866617556 1102 42-43 0.8025713917922346 1102 44-45 0.8329151801771388 1102 46-47 0.8439519821584796 1102 48-49 0.8098465647777147 1102 50-51 0.8445194390019424 1102 52-53 0.7994511191274682 1102 54-55 0.8910497247474183 1102 56-57 0.7165201845412952 1102 58-59 0.7250829787238899 1102 60-61 0.8106867982274224 1102 62-63 0.7587251908438475 1102 64-65 0.6851330015379631 1102 66-67 0.7560237723111882 1102 68-69 0.7537372805052485 1102 70-71 0.6726899688373251 1102 72-73 0.5934291668859188 1102 74-75 0.5871213662393018 1102 76-77 0.6176634934631409 1102 78-79 0.6316756492380762 1102 80-81 0.6449452682685859 1102 82-83 0.6169947583179436 1102 84-85 0.5630899119053865 1102 86-87 0.5677334122233617 1102 88-89 0.5974513036123845 1102 90-91 0.6653250513541877 1102 92-93 0.7404999256398632 1102 94-95 0.6676383950840403 1102 96-97 0.6618596123275928 1102 98-99 0.700494013432202 1102 100 0.7208633803407167 1103 1 0.586873346072263 1103 2 0.7808301687702652 1103 3 0.697821603204666 1103 4 0.7136392622635341 1103 5 0.7203153328841907 1103 6 0.5550226337670026 1103 7 0.5633507474555373 1103 8 0.5957097592226503 1103 9 0.7565056474893694 1103 10-11 0.808606436005114 1103 12-13 0.6686802004996224 1103 14-15 0.798898180080208 1103 16-17 1.0469947780897328 1103 18-19 1.139096180008309 1103 20-21 0.7889197403434238 1103 22-23 1.085590340067938 1103 24-25 0.9987898967408029 1103 26-27 0.8000174962601676 1103 28-29 1.0188165515587073 1103 30-31 1.09107063041683 1103 32-33 0.8923011190828092 1103 34-35 0.8597979365701534 1103 36-37 0.8186462580337732 1103 38-39 0.9469175698602683 1103 40-41 0.8261494359209252 1103 42-43 0.932647181731717 1103 44-45 0.9306256065583369 1103 46-47 0.9071119663838516 1103 48-49 0.8028134922395864 1103 50-51 0.905857150935276 1103 52-53 0.9482228220050146 1103 54-55 0.8449671035012045 1103 56-57 0.8166015373690243 1103 58-59 0.8208069342500437 1103 60-61 0.8247688719040696 1103 62-63 0.7629044812984915 1103 64-65 0.8189542561859753 1103 66-67 0.8620831352488381 1103 68-69 0.8136980191635672 1103 70-71 0.7206459976488233 1103 72-73 0.7567189596346253 1103 74-75 0.6862483002988995 1103 76-77 0.675182530798935 1103 78-79 0.6769696346325986 1103 80-81 0.6889265264519366 1103 82-83 0.5921018435975895 1103 84-85 0.6015311539568273 1103 86-87 0.6470525255910999 1103 88-89 0.6605567605594835 1103 90-91 0.6777097508297771 1103 92-93 0.6962893721701313 1103 94-95 0.6509371512326041 1103 96-97 0.6180932307509757 1103 98-99 0.7155717309662641 1103 100 0.695090330698946 1104 1 0.5805528990007431 1104 2 0.7099918530127312 1104 3 0.6834431584596565 1104 4 0.7507836215717845 1104 5 0.6900077356669811 1104 6 0.48674337711373994 1104 7 0.506510559701951 1104 8 0.486319635224163 1104 9 0.6352342765748915 1104 10-11 0.6288328486922197 1104 12-13 0.5244708659731288 1104 14-15 0.7469986370835215 1104 16-17 0.8696835759004102 1104 18-19 0.8519932171826667 1104 20-21 0.7859573343930819 1104 22-23 0.9645689428445792 1104 24-25 0.8067022224425102 1104 26-27 0.7599934085539743 1104 28-29 0.8912925333625168 1104 30-31 0.863562350333396 1104 32-33 0.7309249973881649 1104 34-35 0.6395051719996232 1104 36-37 0.6806386422560848 1104 38-39 0.7961252765412894 1104 40-41 0.6548759823295285 1104 42-43 0.581187949161972 1104 44-45 0.6969031116645681 1104 46-47 0.6144245860525217 1104 48-49 0.5603369449129332 1104 50-51 0.6476717322547785 1104 52-53 0.6350197729660039 1104 54-55 0.6543793343020994 1104 56-57 0.5540007911516511 1104 58-59 0.4712858397755184 1104 60-61 0.501551210551952 1104 62-63 0.49498153551828494 1104 64-65 0.4674727489406152 1104 66-67 0.5914381464685334 1104 68-69 0.501302387769897 1104 70-71 0.4512835391541543 1104 72-73 0.4090654125735256 1104 74-75 0.4456378524792939 1104 76-77 0.4833530245024775 1104 78-79 0.43114384903584835 1104 80-81 0.43690708002012 1104 82-83 0.4382013989119713 1104 84-85 0.42468684911293764 1104 86-87 0.40500948982666074 1104 88-89 0.3774079565983435 1104 90-91 0.3612453451423576 1104 92-93 0.35190735907035275 1104 94-95 0.34288415827897367 1104 96-97 0.3713149430933136 1104 98-99 0.37718647392668814 1104 100 0.43453063934222413 1105 1 0.44744675378191445 1105 2 0.6616335791676633 1105 3 0.635384991139599 1105 4 0.7418427875117644 1105 5 0.6616421824740257 1105 6 0.3109719661279229 1105 7 0.3378340559210997 1105 8 0.4124326092042381 1105 9 0.4372934408491176 1105 10-11 0.5735303872065529 1105 12-13 0.5364235094008549 1105 14-15 0.6794588338757848 1105 16-17 0.7675897014633364 1105 18-19 0.8433371126499054 1105 20-21 0.6105796453444938 1105 22-23 0.8267641409736015 1105 24-25 0.658295027465094 1105 26-27 0.48675351781081844 1105 28-29 0.7625481355036783 1105 30-31 0.7515874274370091 1105 32-33 0.6686365065868856 1105 34-35 0.5714648199257226 1105 36-37 0.5170260847146722 1105 38-39 0.5823876208244627 1105 40-41 0.3944152616574286 1105 42-43 0.469126386201026 1105 44-45 0.5452632282507395 1105 46-47 0.45475753501297334 1105 48-49 0.5195077259936198 1105 50-51 0.6058624873996763 1105 52-53 0.5519136485691121 1105 54-55 0.5089294576928793 1105 56-57 0.5331286769675003 1105 58-59 0.42319614804841166 1105 60-61 0.46395684555012195 1105 62-63 0.44522984748415695 1105 64-65 0.4430991247498639 1105 66-67 0.44849728159205426 1105 68-69 0.4190483489394907 1105 70-71 0.3933762121656841 1105 72-73 0.3869491878732134 1105 74-75 0.36573715019712694 1105 76-77 0.36096864453384825 1105 78-79 0.4401914059151153 1105 80-81 0.4486776876957492 1105 82-83 0.43346515867015967 1105 84-85 0.3837591216270724 1105 86-87 0.49513918548495184 1105 88-89 0.45807115807886234 1105 90-91 0.52327599794328 1105 92-93 0.5596373768856004 1105 94-95 0.49197810226131367 1105 96-97 0.4988595603320434 1105 98-99 0.5526039016350204 1105 100 0.5869905425452195 1106 1 0.4182238393631259 1106 2 0.5511184840204706 1106 3 0.4936874848374089 1106 4 0.6708834307337952 1106 5 0.5791517720546224 1106 6 0.32045360800082534 1106 7 0.3978228023021231 1106 8 0.34719217396666124 1106 9 0.49480350238192017 1106 10-11 0.4167507734261946 1106 12-13 0.3503071634175754 1106 14-15 0.5484862176819618 1106 16-17 0.6931352944875542 1106 18-19 0.6935769335359012 1106 20-21 0.48846241892623965 1106 22-23 0.79474389259655 1106 24-25 0.6086689885599625 1106 26-27 0.5011706609051032 1106 28-29 0.7328759510647558 1106 30-31 0.6945209602922162 1106 32-33 0.5866837351696077 1106 34-35 0.5278252302963011 1106 36-37 0.49780943179972326 1106 38-39 0.6426367342866541 1106 40-41 0.5719592671320726 1106 42-43 0.5716651635844201 1106 44-45 0.5615279014889296 1106 46-47 0.5465043318407936 1106 48-49 0.5401944286588503 1106 50-51 0.5537988560366571 1106 52-53 0.5440370917371098 1106 54-55 0.5565923381636537 1106 56-57 0.5390488291639528 1106 58-59 0.5606027950883714 1106 60-61 0.4829860195198492 1106 62-63 0.4747458401542417 1106 64-65 0.48670736617457067 1106 66-67 0.5853762779287308 1106 68-69 0.49967981692241636 1106 70-71 0.4726590360347487 1106 72-73 0.412996555600472 1106 74-75 0.4682730592503077 1106 76-77 0.4227894402127177 1106 78-79 0.4843194050493338 1106 80-81 0.570342551993221 1106 82-83 0.5333375688324367 1106 84-85 0.4592492806098676 1106 86-87 0.5020035675774608 1106 88-89 0.5004332015668176 1106 90-91 0.49071652382823316 1106 92-93 0.5634660484703282 1106 94-95 0.5211310172171508 1106 96-97 0.49972650805438334 1106 98-99 0.5097118069972133 1106 100 0.5401667808908464 1107 1 0.5847041963611765 1107 2 0.7159999587151553 1107 3 0.631810445184275 1107 4 0.7118827331820086 1107 5 0.5624616855606206 1107 6 0.41542219799811875 1107 7 0.37236333191700766 1107 8 0.4609030405648227 1107 9 0.569953350949838 1107 10-11 0.5683593181733997 1107 12-13 0.47987280933480037 1107 14-15 0.7102408932760014 1107 16-17 0.8566024819175837 1107 18-19 0.9630304386227095 1107 20-21 0.6389091909639788 1107 22-23 0.9163074010763026 1107 24-25 0.5639556161814809 1107 26-27 0.583570146274063 1107 28-29 0.7758671491634104 1107 30-31 0.767757120505614 1107 32-33 0.6543334495929543 1107 34-35 0.5097585985677426 1107 36-37 0.5234828274810326 1107 38-39 0.5586879900210846 1107 40-41 0.47490663525584864 1107 42-43 0.5748791264007593 1107 44-45 0.5325832541802313 1107 46-47 0.47762391658567793 1107 48-49 0.5530516718002332 1107 50-51 0.6161176045262664 1107 52-53 0.6625142462099376 1107 54-55 0.5527393428665874 1107 56-57 0.4920498147735657 1107 58-59 0.5802254232004742 1107 60-61 0.5741051241683479 1107 62-63 0.521798169448715 1107 64-65 0.5056570121337529 1107 66-67 0.55911171819929 1107 68-69 0.5778783984998697 1107 70-71 0.49044573930170543 1107 72-73 0.47961313490170454 1107 74-75 0.540933043298196 1107 76-77 0.5225764024896478 1107 78-79 0.501795881335596 1107 80-81 0.5631475211117234 1107 82-83 0.6080237209568224 1107 84-85 0.5483371943248514 1107 86-87 0.5657586716272576 1107 88-89 0.6024881350815399 1107 90-91 0.5954455538036001 1107 92-93 0.7208993927369569 1107 94-95 0.6819846850525906 1107 96-97 0.6872972768823296 1107 98-99 0.6098029446198616 1107 100 0.5759236729970532 1108 1 0.34275826792696407 1108 2 0.515904006352681 1108 3 0.4582772597816245 1108 4 0.5933070101776963 1108 5 0.45368384329473344 1108 6 0.26615859400906317 1108 7 0.22211439668249966 1108 8 0.28844593598677193 1108 9 0.4528617002288158 1108 10-11 0.4469517606375675 1108 12-13 0.28607015152532256 1108 14-15 0.4774283045042864 1108 16-17 0.5938684221667785 1108 18-19 0.6805024701552895 1108 20-21 0.39879280829047303 1108 22-23 0.6241252472997729 1108 24-25 0.475488165119927 1108 26-27 0.4117678456041034 1108 28-29 0.5723374010635283 1108 30-31 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-3.9063193032901893 2215 94-95 -2.083162844806232 2215 96-97 -1.6427358804020038 2215 98-99 -3.1644207921994685 2215 100 -3.150644149012262 2216 1 -0.7149350844620592 2216 2 -0.6431848426225244 2216 3 -0.8871443630874829 2216 4 -0.8393016238015676 2216 5 -1.0222512790079108 2216 6 -0.8184912576693009 2216 7 -0.9719108608446732 2216 8 -0.8333420163441545 2216 9 -1.132181977570589 2216 10-11 -1.0967638517277436 2216 12-13 -1.2333205159568905 2216 14-15 -1.6476555598744014 2216 16-17 -1.3368869938381422 2216 18-19 -3.198124312671382 2216 20-21 -1.6148623043174481 2216 22-23 -1.0720035476535585 2216 24-25 -1.2107296777880308 2216 26-27 -1.203150653818156 2216 28-29 -1.17600621549105 2216 30-31 -1.2954102741757936 2216 32-33 -1.306837676309165 2216 34-35 -1.502440434643546 2216 36-37 -1.493306769854918 2216 38-39 -1.6069161553726943 2216 40-41 -1.6106109646108422 2216 42-43 -1.5846485661969893 2216 44-45 -1.452853059942278 2216 46-47 -1.612528583274468 2216 48-49 -1.6005733379299727 2216 50-51 -1.4495005264749778 2216 52-53 -1.409625507934038 2216 54-55 -1.3303561289620234 2216 56-57 -1.4360477583801199 2216 58-59 -1.5262889433530376 2216 60-61 -1.4187467970161336 2216 62-63 -1.452392452091587 2216 64-65 -1.412983841647275 2216 66-67 -1.4780319449468493 2216 68-69 -1.5317116868292544 2216 70-71 -1.5879721689650523 2216 72-73 -1.5575817026273455 2216 74-75 -1.5570741519733318 2216 76-77 -1.4104196371446633 2216 78-79 -1.5905897666638857 2216 80-81 -1.4824131086367913 2216 82-83 -1.610024551188566 2216 84-85 -1.5192295747569453 2216 86-87 -1.5628677466178615 2216 88-89 -1.4510963611807668 2216 90-91 -1.491890135217016 2216 92-93 -1.437124563364364 2216 94-95 -1.3684484706121012 2216 96-97 -1.3488607941271233 2216 98-99 -1.3201138026921662 2216 100 -1.262545925278566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18246.0 1 3063.0 2 495.0 3 465.0 4 458.0 5 254.0 6 231.0 7 299.0 8 511.0 9 891.0 10 1647.0 11 3185.0 12 5308.0 13 7494.0 14 9970.0 15 12340.0 16 15010.0 17 17733.0 18 21204.0 19 23851.0 20 27217.0 21 31118.0 22 35587.0 23 41268.0 24 46071.0 25 51673.0 26 58438.0 27 67088.0 28 78708.0 29 94097.0 30 111287.0 31 133270.0 32 164926.0 33 209478.0 34 282978.0 35 383877.0 36 563208.0 37 877617.0 38 818735.0 39 273982.0 40 386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.924905005995775 20.50061081622355 12.507021295964343 24.067462881816333 2 15.32250980213337 19.81511793965315 41.02297591897305 23.83939633924043 3 18.58753681596979 26.472370469947528 28.93097961560237 26.009113098480313 4 11.971092418187407 16.70708436967275 39.97589916999693 31.34592404214292 5 13.01610396112074 38.47703528880634 35.269094050217184 13.237766699855733 6 30.03369391577505 38.31616288284678 18.328613621036023 13.321529580342146 7 27.111080893456524 31.83764143651549 23.49219370629871 17.55908396372927 8 25.761099713293888 35.563746874670805 20.165533134919613 18.50962027711569 9 26.06504769931981 15.3295691045126 21.290284048509967 37.31509914765763 10-11 25.23252887384994 26.041354624010737 28.153002041444115 20.573114460695212 12-13 25.543592247507924 23.870462933600006 27.94918474446117 22.636760074430903 14-15 22.97782385209369 25.23160136310973 25.214442806638726 26.576131978157854 16-17 22.171582888043893 27.85010130663353 26.479919719820295 23.498396085502286 18-19 21.757424626759406 28.208754997412157 27.920775643781226 22.113044732047207 20-21 23.234593926597512 27.3928615412287 27.001094071955507 22.371450460218277 22-23 23.52133812091969 27.145068987691158 26.728527506351885 22.605065385037268 24-25 22.969909968154965 27.255331833734704 27.35584446380773 22.418913734302592 26-27 22.89227699896724 27.426950516408137 26.604343185095598 23.07642929952902 28-29 22.26227408199307 27.74960876089293 27.0719791604573 22.916137996656698 30-31 22.964723176941757 27.473722083470854 26.942074002379755 22.61948073720764 32-33 23.185547775432283 27.68628807144779 26.247268487771752 22.880895665348174 34-35 23.222137781483525 27.53436125328948 26.97568397557637 22.267816989650626 36-37 22.97121572048747 27.209037745320753 26.721168032165593 23.09857850202618 38-39 23.034093803629364 27.355385223076347 27.173311762542916 22.437209210751373 40-41 22.766906381942363 26.619996713204984 27.67078664594651 22.942310258906147 42-43 22.71195120977138 26.805886517769373 27.142803285780303 23.339358986678942 44-45 22.808437244878153 27.613692392738482 26.990072085540028 22.587798276843333 46-47 22.791617575341455 27.361571972572328 26.634048881702316 23.2127615703839 48-49 23.273503735300082 26.997384232988146 26.886264186540306 22.84284784517147 50-51 22.98814276471046 26.905070971587204 26.85361310305266 23.253173160649677 52-53 22.57965252763156 27.450878624938184 26.68627431460387 23.283194532826386 54-55 22.59293212971876 27.34441674492697 26.730893252572802 23.331757872781466 56-57 23.432071835227013 26.912953845537796 26.48078950832904 23.17418481090615 58-59 22.58652791550542 27.135393682929436 27.209668732161823 23.068409669403323 60-61 22.613894600454092 27.659486890853323 26.685977430409725 23.04064107828286 62-63 22.87424238865532 27.117827787489666 26.431098330934255 23.576831492920757 64-65 22.27666316284365 27.24141668695991 26.77022948828934 23.711690661907106 66-67 22.82292164663345 27.02151882564469 26.605455534270412 23.550103993451444 68-69 22.423137389859832 27.03675120461088 26.840726431715893 23.69938497381339 70-71 22.47732334987995 26.702449060315057 27.019074248578683 23.80115334122631 72-73 22.89733839368425 26.845779105100053 27.292089168940613 22.964793332275086 74-75 23.01856826176626 27.134945163709062 26.86973222206922 22.97675435245546 76-77 22.757029608908116 27.051292676742513 26.683741222391 23.50793649195837 78-79 22.627687410375835 27.072777536051333 26.70391503978678 23.595620013786053 80-81 22.340304278413956 27.328761599584084 27.165847585187024 23.165086536814933 82-83 23.044665555296056 26.578334178941404 27.851611890054844 22.525388375707696 84-85 23.22826476165909 26.98621526619921 26.797100115028456 22.988419857113247 86-87 22.18127352457047 27.532142321329967 27.24202909593048 23.04455505816908 88-89 23.03296816554833 26.525810555915857 27.081933443479254 23.35928783505656 90-91 22.77902021524132 26.599656047707796 27.223218647317495 23.398105089733388 92-93 22.984451684521787 26.778921992010563 27.09320495152887 23.14342137193878 94-95 22.13345916475568 26.752363445791794 27.60582091839387 23.50835647105866 96-97 22.453133223216067 26.695156752850192 27.23747416103086 23.61423586290288 98-99 21.978024821383755 27.24497805103347 27.12734990514712 23.649647222435657 100 22.631681848258182 26.745684287542126 26.497998648741987 24.124635215457708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15737.0 1 9361.0 2 2783.5 3 2085.5 4 1221.0 5 731.0 6 571.5 7 533.5 8 556.0 9 534.5 10 476.0 11 447.5 12 429.5 13 442.5 14 506.0 15 601.5 16 689.5 17 785.5 18 937.5 19 1205.5 20 1633.0 21 2256.0 22 3103.0 23 4394.0 24 5961.5 25 7963.0 26 11502.0 27 15690.5 28 20842.5 29 29974.0 30 42315.0 31 53702.0 32 68147.0 33 82474.0 34 94115.5 35 106940.0 36 119139.0 37 138165.5 38 146431.0 39 139890.0 40 133386.0 41 125104.5 42 123543.5 43 131267.0 44 141494.5 45 160975.0 46 179374.5 47 190391.5 48 199167.5 49 197352.5 50 189884.0 51 190446.5 52 189278.5 53 180983.5 54 170794.0 55 151539.5 56 135175.5 57 115198.5 58 94682.5 59 87143.5 60 73066.0 61 54480.5 62 42221.5 63 34015.5 64 24980.5 65 14750.0 66 9558.5 67 6749.0 68 4708.5 69 3420.5 70 2581.0 71 1999.5 72 1606.5 73 1324.0 74 1022.0 75 770.0 76 578.0 77 397.5 78 240.5 79 153.0 80 102.0 81 64.0 82 52.5 83 39.5 84 24.5 85 20.5 86 21.5 87 19.0 88 15.5 89 16.0 90 17.5 91 12.0 92 10.0 93 9.0 94 4.5 95 3.5 96 3.0 97 1.0 98 0.5 99 1.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9947116651356221 2 0.4426899741502703 3 0.4766711529834006 4 0.46262915963454326 5 0.42947136234484823 6 0.4815669351335569 7 0.4984796371068242 8 0.5014838670626018 9 0.5153923390800914 10-11 0.5293898253184929 12-13 0.5284440492213036 14-15 0.5249836213833522 16-17 0.5300463051977183 18-19 0.527976724561516 20-21 0.526641511247837 22-23 0.4886991995841255 24-25 0.5247165787206164 26-27 0.5037426029182422 28-29 0.5103296552657253 30-31 0.5126662785646635 32-33 0.5152810713039515 34-35 0.5280991191152699 36-37 0.5461133720723222 38-39 0.5349643409031027 40-41 0.5399713908293989 42-43 0.5377015281961446 44-45 0.5474040782755453 46-47 0.5458240758543585 48-49 0.5179181175984675 50-51 0.5338182828088616 52-53 0.5260629188119095 54-55 0.5356764546703981 56-57 0.5227248855277119 58-59 0.5294454592065628 60-61 0.5217457290976807 62-63 0.5162602277339828 64-65 0.529289684319967 66-67 0.5267639058015908 68-69 0.5471259088351955 70-71 0.5311478561815035 72-73 0.5343857484671751 74-75 0.5309587009620657 76-77 0.5178291033775556 78-79 0.5486502773683124 80-81 0.5232812244084115 82-83 0.5231365762994297 84-85 0.531871096726413 86-87 0.8415738248342556 88-89 1.1096067707776995 90-91 1.1002825311371744 92-93 1.099781826144545 94-95 1.1042881710782115 96-97 1.0973450618470806 98-99 1.0937511126777613 100 1.0885771610872552 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4493664.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.28653978174634 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07697508449135 28.74437593265557 2 12.898323000294987 9.876643133372157 3 4.796335669252738 5.509052892223618 4 2.347017185034037 3.5943666729299184 5 1.2601710768393517 2.4123795032607984 6 0.757036483979756 1.7390584476074586 7 0.5175265178976468 1.387000973091784 8 0.37752614717728367 1.1563335882041996 9 0.26494041260189566 0.9129286482187292 >10 1.358161369305669 9.653231482049758 >50 0.1489961107254996 4.0054023249115955 >100 0.15637698548451115 12.80881751795886 >500 0.026591086423951176 7.102661081253983 >1k 0.013441008645308646 9.147287883096327 >5k 4.073032922820802E-4 1.1435474349319437 >10k+ 1.7455855383517721E-4 0.8069124842334203 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 13617 0.3030266615394476 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 12147 0.2703139353543122 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 10457 0.23270542701902056 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 9526 0.2119873671017682 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 8423 0.18744169568530267 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8275 0.18414816951156115 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 7638 0.16997265483133586 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6684 0.14874276314383986 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5582 0.12421934528260235 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5204 0.11580750140642469 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 4885 0.10870861728869805 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4692 0.10441368112969727 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4659 0.10367931380717384 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2253555227983224E-5 2 0.0 0.0 0.0 0.0 2.2253555227983224E-5 3 0.0 0.0 0.0 0.0 2.2253555227983224E-5 4 0.0 0.0 0.0 0.0 2.2253555227983224E-5 5 0.0 0.0 0.0 0.0 2.2253555227983224E-5 6 0.0 0.0 0.0 0.0 8.90142209119329E-5 7 0.0 0.0 0.0 0.0 1.1126777613991611E-4 8 0.0 0.0 0.0 0.0 1.1126777613991611E-4 9 0.0 0.0 0.0 0.0 1.1126777613991611E-4 10-11 0.0 0.0 0.0 0.0 1.2239455375390773E-4 12-13 0.0 0.0 0.0 0.0 2.670426627357987E-4 14-15 0.0 0.0 0.0 0.0 3.2267655080575673E-4 16-17 0.0 0.0 0.0 1.1126777613991612E-5 3.7831043887571477E-4 18-19 0.0 0.0 0.0 2.2253555227983224E-5 4.00563994103698E-4 20-21 0.0 0.0 0.0 2.2253555227983224E-5 4.784514374016393E-4 22-23 0.0 0.0 0.0 7.788744329794128E-5 4.895782150156309E-4 24-25 0.0 0.0 0.0 1.4464810898189094E-4 4.895782150156309E-4 26-27 0.0 0.0 0.0 3.7831043887571477E-4 5.45212103085589E-4 28-29 0.0 0.0 0.0 0.0010570438733292031 5.563388806995806E-4 30-31 0.0 0.0 0.0 0.0048178947068583675 5.563388806995806E-4 32-33 0.0 0.0 0.0 0.014754107116152876 5.563388806995806E-4 34-35 0.0 0.0 0.0 0.030921314989282685 6.00845991155547E-4 36-37 0.0 0.0 0.0 0.05965065478860902 6.00845991155547E-4 38-39 0.0 0.0 0.0 0.1063719939897598 6.787334344534883E-4 40-41 0.0 0.0 0.0 0.15968038553839362 6.898602120674799E-4 42-43 0.0 0.0 0.0 0.21731709357887016 6.898602120674799E-4 44-45 0.0 0.0 0.0 0.28709311599621157 7.121137672954632E-4 46-47 0.0 0.0 0.0 0.37705311300533373 7.566208777514295E-4 48-49 0.0 0.0 0.0 0.4812887656932071 8.01127988207396E-4 50-51 0.0 0.0 0.0 0.5959279554501626 8.01127988207396E-4 52-53 0.0 0.0 0.0 0.7113682731953257 8.456350986633625E-4 54-55 0.0 0.0 0.0 0.8427866435941807 8.678886538913457E-4 56-57 0.0 0.0 0.0 1.002778578905766 8.790154315053372E-4 58-59 0.0 0.0 0.0 1.1926792924437608 8.901422091193289E-4 60-61 0.0 0.0 0.0 1.3990365100728492 8.901422091193289E-4 62-63 0.0 0.0 0.0 1.6110906378403014 8.901422091193289E-4 64-65 0.0 0.0 0.0 1.8368307020729633 9.45776097189287E-4 66-67 0.0 0.0 0.0 2.0960289865908974 9.569028748032786E-4 68-69 0.0 0.0 0.0 2.391200143134867 9.791564300312618E-4 70-71 0.0 0.0 0.0 2.7224888198138535 0.0010236635404872283 72-73 0.0 0.0 0.0 3.070467662913827 0.001112677761399161 74-75 0.0 0.0 0.0 3.4146634016250434 0.001257325870381052 76-77 0.0 0.0 0.0 3.8011742756022704 0.0013018329808370185 78-79 0.0 0.0 0.0 4.2259390110164 0.0013129597584510102 80-81 0.0 0.0 0.0 4.694576630562499 0.0013352133136789934 82-83 0.0 0.0 0.0 5.1827083644883105 0.0015243685331168507 84-85 0.0 0.0 0.0 5.662928959530575 0.001546622088344834 86-87 0.0 0.0 0.0 6.161697892855363 0.0016690166420987415 88 0.0 0.0 0.0 6.568426121757212 0.0017802844182386577 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 3940 0.0 34.601494 1 GTATCAA 8320 0.0 33.39529 1 TAGGCAT 4645 0.0 32.666496 5 CAACGCA 7905 0.0 31.734022 5 GCATCGT 4045 0.0 31.339905 8 GGTATCA 5260 0.0 30.940449 1 ATCAACG 8390 0.0 29.96194 3 GGGCACC 4115 0.0 29.370749 94 CTAGGCA 5395 0.0 28.911543 4 TAGTACT 4835 0.0 27.401686 4 CTGTGCG 4610 0.0 27.296423 9 TATCAAC 10095 0.0 27.229969 2 CTAACGC 1850 0.0 27.176292 3 GTACATG 17320 0.0 26.58246 1 AACGCAG 9855 0.0 26.342842 6 TAACGCC 1925 0.0 25.870197 4 TACATGG 17915 0.0 25.363386 2 ACATGGG 17375 0.0 25.258074 3 ACGCAGA 9800 0.0 25.152199 7 TCTAACG 1910 0.0 25.093683 2 >>END_MODULE