FastQCFastQC Report
Mon 16 Jan 2017
ERR1146436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4493664
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC136170.3030266615394476No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA102410.2278986590897762No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT102210.22745358798521653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95330.21214314198836404No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA88850.19772283820063097No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC88340.1965879068840038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88300.19649889266309187No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC86850.19327212715503428No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC73880.16440926602434006No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG57980.12902611321184673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54390.12103708688500074No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT52810.1175210251589794No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC49920.11108974769809224No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT49380.10988805571578117No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG48400.10770720730343879No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85350.045.8326721
GTATCAA144050.037.013081
GTACTAG36950.031.0477981
GGGCACC41500.030.26468894
CAACGCA170800.029.7722135
TAGGCAT47100.029.1362515
ATCAACG174550.029.0858653
GTGGTAT29150.028.226361
TATCAAC189300.028.0624282
CTGTGCG48650.027.2473959
AACGCAG192000.027.2433726
TAGTACT51450.025.8564834
TCAACGC201000.025.5618634
TGAACCG5800.025.1191795
ACGCAGA204950.024.719397
CTAGGCA55100.024.6555484
CGCAGAG207600.024.3812128
GTACTGT59550.024.2283336
TACTGTG69150.023.9910587
ACTGTGC61700.023.9172578
Produced by FastQC (version 0.11.5)