##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146436_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4493664 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84622170237917 31.0 31.0 34.0 30.0 34.0 2 31.837716171035485 31.0 31.0 34.0 30.0 34.0 3 32.00395089619518 33.0 31.0 34.0 30.0 34.0 4 35.75031511034203 37.0 35.0 37.0 35.0 37.0 5 35.650355700826765 37.0 35.0 37.0 33.0 37.0 6 35.68337485846739 37.0 35.0 37.0 33.0 37.0 7 35.61410666218035 37.0 35.0 37.0 33.0 37.0 8 35.59511992885984 37.0 35.0 37.0 33.0 37.0 9 37.20329690871414 39.0 37.0 39.0 33.0 39.0 10-11 37.14282532027317 39.0 37.0 39.0 33.0 39.0 12-13 37.08404288794178 39.0 37.0 39.0 33.0 39.0 14-15 38.293871993989754 40.0 38.0 41.0 33.0 41.0 16-17 38.2949331547708 40.0 38.0 41.0 33.0 41.0 18-19 38.35763110014456 40.0 38.0 41.0 33.5 41.0 20-21 38.376343669664664 40.0 38.0 41.0 33.5 41.0 22-23 38.34205572557272 40.0 38.0 41.0 33.0 41.0 24-25 38.280439525518595 40.0 38.0 41.0 33.0 41.0 26-27 38.14695546885571 40.0 37.0 41.0 33.0 41.0 28-29 38.08141707969265 40.0 37.0 41.0 33.0 41.0 30-31 37.95359577396084 40.0 37.0 41.0 33.0 41.0 32-33 37.830306293483446 40.0 37.0 41.0 33.0 41.0 34-35 37.73650110466648 40.0 36.5 41.0 32.5 41.0 36-37 37.654433442286745 40.0 36.0 41.0 32.0 41.0 38-39 37.54671655468678 40.0 36.0 41.0 32.0 41.0 40-41 37.427283504062615 40.0 36.0 41.0 31.5 41.0 42-43 37.308117051030074 40.0 36.0 41.0 31.0 41.0 44-45 37.13054369441062 39.0 35.5 41.0 31.0 41.0 46-47 36.90624599436006 39.0 35.0 41.0 30.5 41.0 48-49 36.66660947502973 39.0 35.0 41.0 30.0 41.0 50-51 36.48581803178876 39.0 35.0 41.0 30.0 41.0 52-53 36.31749102736653 39.0 35.0 41.0 29.5 41.0 54-55 36.129878869448184 38.5 35.0 40.5 29.0 41.0 56-57 35.902289534776074 38.0 35.0 40.0 29.0 41.0 58-59 35.65031597823068 38.0 34.0 40.0 28.0 41.0 60-61 35.39971212800957 37.0 34.0 40.0 28.0 41.0 62-63 35.13432568612161 37.0 34.0 40.0 28.0 41.0 64-65 34.870794078061905 36.0 34.0 39.5 28.0 41.0 66-67 34.64395991778647 36.0 34.0 39.0 28.0 41.0 68-69 34.441545140001566 35.0 33.5 39.0 27.5 41.0 70-71 34.20191707702223 35.0 33.0 38.5 27.0 41.0 72-73 33.978035518454426 35.0 33.0 37.5 27.0 40.0 74-75 33.77794011746317 35.0 33.0 37.0 27.0 39.5 76-77 33.592791428108555 35.0 33.0 37.0 27.0 39.0 78-79 33.45039671412905 35.0 33.0 36.0 27.0 39.0 80-81 33.27625363178021 35.0 33.0 36.0 27.0 38.5 82-83 33.07219175710512 35.0 33.0 36.0 27.0 37.0 84-85 32.907015188496516 35.0 33.0 35.0 27.0 37.0 86-87 32.75630087162725 35.0 33.0 35.0 27.0 36.5 88-89 32.630406389974866 35.0 33.0 35.0 27.0 36.0 90-91 32.53172366692303 35.0 33.0 35.0 26.5 36.0 92-93 32.45199885883768 35.0 33.0 35.0 26.0 36.0 94-95 32.39421639001047 35.0 33.0 35.0 26.0 35.5 96-97 32.335030611990575 35.0 33.0 35.0 26.0 35.0 98-99 32.29236687478192 35.0 33.0 35.0 26.0 35.0 100 31.80554220342242 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4405529923520426 1101 2 0.5705178283085068 1101 3 0.42475548104223293 1101 4 0.3263820483648203 1101 5 0.33357872543273714 1101 6 0.36396046215475764 1101 7 0.37682405577462674 1101 8 0.36986598610985766 1101 9 0.45744909369692976 1101 10-11 0.43128408006460717 1101 12-13 0.508600702863049 1101 14-15 0.6164697580709557 1101 16-17 0.5902996349120926 1101 18-19 0.5618818143033977 1101 20-21 0.5924101180415988 1101 22-23 0.5897514192947568 1101 24-25 0.6598786855605354 1101 26-27 0.6307101848339656 1101 28-29 0.6344147427732594 1101 30-31 0.5986394537260793 1101 32-33 0.6879536513121423 1101 34-35 0.6567586525136093 1101 36-37 0.6355104371231519 1101 38-39 0.6439572422323181 1101 40-41 0.6393963152642712 1101 42-43 0.7050683043111547 1101 44-45 0.6718414204562535 1101 46-47 0.7158490357950811 1101 48-49 0.7598046403757976 1101 50-51 0.7542725760750812 1101 52-53 0.7503774753621997 1101 54-55 0.7653930038588257 1101 56-57 0.7143979371526896 1101 58-59 0.7719396527273759 1101 60-61 0.8286287066319815 1101 62-63 0.7835414946815575 1101 64-65 0.776734206804548 1101 66-67 0.692840740304419 1101 68-69 0.7344382865878387 1101 70-71 0.6602478421622635 1101 72-73 0.6454387994933057 1101 74-75 0.6033222362894577 1101 76-77 0.6054405649690722 1101 78-79 0.583618116078263 1101 80-81 0.5549488648220873 1101 82-83 0.5293065037474491 1101 84-85 0.5360222920051569 1101 86-87 0.5343073595611045 1101 88-89 0.5314224642873384 1101 90-91 0.5470344653209835 1101 92-93 0.5172205475336185 1101 94-95 0.4482556958263544 1101 96-97 0.44477799821489583 1101 98-99 0.5004424204393629 1101 100 0.5148866733522084 1102 1 0.4803208568619475 1102 2 0.561111075592752 1102 3 0.45931518297823715 1102 4 0.3279672508308593 1102 5 0.3291779134375048 1102 6 0.37590871282687033 1102 7 0.40680343486602055 1102 8 0.4050041356104188 1102 9 0.4776214172819522 1102 10-11 0.47145485144641697 1102 12-13 0.49638352743574643 1102 14-15 0.6282602910657786 1102 16-17 0.6496114496545076 1102 18-19 0.6106589676139365 1102 20-21 0.5948014544253795 1102 22-23 0.5326771507256893 1102 24-25 0.5736087169529398 1102 26-27 0.6222993011061178 1102 28-29 0.6285646741621207 1102 30-31 0.6615524780941371 1102 32-33 0.6601324792945604 1102 34-35 0.5979528193032237 1102 36-37 0.6040387486948973 1102 38-39 0.6333764970996398 1102 40-41 0.5814567873253011 1102 42-43 0.6372757011997834 1102 44-45 0.7039795829345294 1102 46-47 0.6375106505440513 1102 48-49 0.6326926877622228 1102 50-51 0.5798279668477875 1102 52-53 0.6059117329480088 1102 54-55 0.6036065135934834 1102 56-57 0.6087556101934624 1102 58-59 0.5804100930749598 1102 60-61 0.5427508699763592 1102 62-63 0.5127029531856522 1102 64-65 0.5186284701756989 1102 66-67 0.5551049258024392 1102 68-69 0.5251301517111173 1102 70-71 0.4844603380981951 1102 72-73 0.4597692338438151 1102 74-75 0.43985982761159903 1102 76-77 0.47522367231937324 1102 78-79 0.3779907006861407 1102 80-81 0.39031762920002677 1102 82-83 0.4052910599232007 1102 84-85 0.41606428431005327 1102 86-87 0.436177017352378 1102 88-89 0.3867282244126926 1102 90-91 0.41680523530256863 1102 92-93 0.4617488988923171 1102 94-95 0.414103425887113 1102 96-97 0.43834418849690593 1102 98-99 0.44125528142479453 1102 100 0.5606031560085114 1103 1 0.287782251378804 1103 2 -0.1496445746582289 1103 3 0.19899568290065872 1103 4 0.20983439158711548 1103 5 0.261742046363608 1103 6 0.2524827094909199 1103 7 0.3089188040386617 1103 8 0.30191404242576425 1103 9 0.3303692688561455 1103 10-11 0.39836786146786807 1103 12-13 0.42961762920388935 1103 14-15 0.5347623946618256 1103 16-17 0.5278289312360229 1103 18-19 0.5480349733487415 1103 20-21 0.5354732343216355 1103 22-23 0.4826515735191492 1103 24-25 0.5263714635761687 1103 26-27 0.5398510152531983 1103 28-29 0.5890408933784528 1103 30-31 0.6378104632354962 1103 32-33 0.6369023169308647 1103 34-35 0.5720663767411196 1103 36-37 0.5816878174268325 1103 38-39 0.5545022467859155 1103 40-41 0.6086077140678512 1103 42-43 0.6168334586025566 1103 44-45 0.5962870386934327 1103 46-47 0.6361126234447525 1103 48-49 0.7003555108086985 1103 50-51 0.6586688713064746 1103 52-53 0.6258048216282504 1103 54-55 0.5695787060426198 1103 56-57 0.6015493540006602 1103 58-59 0.5726599145215232 1103 60-61 0.6071870136847366 1103 62-63 0.6041146176338046 1103 64-65 0.5761996848426989 1103 66-67 0.6396297460920977 1103 68-69 0.5589977481657016 1103 70-71 0.5601827033162792 1103 72-73 0.5396418607588274 1103 74-75 0.5528179545749197 1103 76-77 0.5498560236342911 1103 78-79 0.5065675859348886 1103 80-81 0.5069588675255758 1103 82-83 0.5114984376255762 1103 84-85 0.4785885174744777 1103 86-87 0.5036707186436118 1103 88-89 0.4196010064029352 1103 90-91 0.5212116710015522 1103 92-93 0.5424272705495667 1103 94-95 0.5215371144218608 1103 96-97 0.5069029580995092 1103 98-99 0.4990947515863837 1103 100 0.4597330078304225 1104 1 0.2939037267366409 1104 2 0.44086691715433446 1104 3 0.3624481757809974 1104 4 0.22159785446714864 1104 5 0.26543871921115425 1104 6 0.273231123902292 1104 7 0.27466394125035265 1104 8 0.31070312789767485 1104 9 0.3566584642325452 1104 10-11 0.36854094818287564 1104 12-13 0.38674739823239435 1104 14-15 0.5076153462347079 1104 16-17 0.4597225000450962 1104 18-19 0.4278123587928562 1104 20-21 0.42645409960937997 1104 22-23 0.44317179368904647 1104 24-25 0.44957937779044244 1104 26-27 0.5272692754300081 1104 28-29 0.5107803472428571 1104 30-31 0.5262913922625856 1104 32-33 0.5166068663890897 1104 34-35 0.4951216942139993 1104 36-37 0.4973933837769877 1104 38-39 0.47965089063798416 1104 40-41 0.5689109371389165 1104 42-43 0.5067220044355665 1104 44-45 0.506158644021852 1104 46-47 0.5351499545630034 1104 48-49 0.4973112142115781 1104 50-51 0.5157230554253971 1104 52-53 0.5290545215811306 1104 54-55 0.4828188445673902 1104 56-57 0.514949412735298 1104 58-59 0.5040372207714512 1104 60-61 0.4586518385305567 1104 62-63 0.43827087589958325 1104 64-65 0.4103636023233719 1104 66-67 0.42251538782360853 1104 68-69 0.37354469563807413 1104 70-71 0.41107900457650004 1104 72-73 0.3869251529789608 1104 74-75 0.34272379134782227 1104 76-77 0.37286582648505373 1104 78-79 0.3360568327644202 1104 80-81 0.37872307744136435 1104 82-83 0.35842194609577405 1104 84-85 0.3468152298152489 1104 86-87 0.3167651327362293 1104 88-89 0.330161920086411 1104 90-91 0.3665596579867696 1104 92-93 0.3561028511783846 1104 94-95 0.3208587073329099 1104 96-97 0.3355182990032901 1104 98-99 0.3334720883682465 1104 100 0.37133916302730796 1105 1 0.0960702072552273 1105 2 -0.4560320931375337 1105 3 -0.009730077458687703 1105 4 0.054817583332258835 1105 5 0.15985069532090534 1105 6 0.18859356713129216 1105 7 0.2365274668034445 1105 8 0.22448318748171658 1105 9 0.33513943340192043 1105 10-11 0.32684729629061593 1105 12-13 0.26461818064569087 1105 14-15 0.4125158194756082 1105 16-17 0.4019006887367951 1105 18-19 0.38752589479856425 1105 20-21 0.3828205827098756 1105 22-23 0.38531256536656144 1105 24-25 0.4045404636792469 1105 26-27 0.4071543820841157 1105 28-29 0.39371808466494684 1105 30-31 0.43633764194088087 1105 32-33 0.37479686153341873 1105 34-35 0.34678140010836245 1105 36-37 0.3954456064350822 1105 38-39 0.40901055580464174 1105 40-41 0.4781362300410592 1105 42-43 0.4844037236942711 1105 44-45 0.4878494495558243 1105 46-47 0.43226472172798225 1105 48-49 0.42781672078006494 1105 50-51 0.44214552662241147 1105 52-53 0.40226787477025994 1105 54-55 0.39883977102682167 1105 56-57 0.4070012331136468 1105 58-59 0.3389052034675899 1105 60-61 0.3120564120265712 1105 62-63 0.3132672737051223 1105 64-65 0.32739677666042155 1105 66-67 0.2967639450718593 1105 68-69 0.35847437372265034 1105 70-71 0.364253297900909 1105 72-73 0.3483853993274195 1105 74-75 0.32154108679522864 1105 76-77 0.25209431347175837 1105 78-79 0.2650409223087209 1105 80-81 0.2695134842769491 1105 82-83 0.24258500223920265 1105 84-85 0.30177308830438676 1105 86-87 0.2661256599309283 1105 88-89 0.32048741479472653 1105 90-91 0.2541846832854162 1105 92-93 0.2729092538395861 1105 94-95 0.26622892963619904 1105 96-97 0.23584051842149023 1105 98-99 0.2645286578131447 1105 100 0.31555522556639914 1106 1 0.24565008842091274 1106 2 0.2921423062219155 1106 3 0.24232417588851263 1106 4 0.18141973901465036 1106 5 0.18847785145083407 1106 6 0.2087738772852603 1106 7 0.2015989756997385 1106 8 0.1800940889267011 1106 9 0.2116893895429186 1106 10-11 0.2620724663154732 1106 12-13 0.27590800975016805 1106 14-15 0.3219970341503924 1106 16-17 0.27236170873943166 1106 18-19 0.361564251493526 1106 20-21 0.3663743299290232 1106 22-23 0.31003706326637115 1106 24-25 0.32188786037460915 1106 26-27 0.35713108961533635 1106 28-29 0.285176848247815 1106 30-31 0.2805323856952455 1106 32-33 0.31472088536538223 1106 34-35 0.29722822899639567 1106 36-37 0.40342747777440024 1106 38-39 0.4006689022793992 1106 40-41 0.31664148823960403 1106 42-43 0.282830820911542 1106 44-45 0.30142517510096667 1106 46-47 0.2883670230645876 1106 48-49 0.23840708416698675 1106 50-51 0.32007081658822756 1106 52-53 0.2721550863301587 1106 54-55 0.2910896851803457 1106 56-57 0.3703511132540527 1106 58-59 0.35042264779757204 1106 60-61 0.36550796938747965 1106 62-63 0.31487321314225625 1106 64-65 0.3288839957064553 1106 66-67 0.3780301277433935 1106 68-69 0.2903790548375653 1106 70-71 0.29289324963158236 1106 72-73 0.28466481293494184 1106 74-75 0.31723796084420997 1106 76-77 0.3420345911749507 1106 78-79 0.2574786205490014 1106 80-81 0.2007783039830784 1106 82-83 0.22762938608932615 1106 84-85 0.22821748582484958 1106 86-87 0.25315500966215865 1106 88-89 0.22623540614986837 1106 90-91 0.22512870161668275 1106 92-93 0.2951908905047418 1106 94-95 0.3382006238957942 1106 96-97 0.25301148229436166 1106 98-99 0.27537446533150245 1106 100 0.35871667878861757 1107 1 0.08855581726167472 1107 2 -0.49454445994170015 1107 3 -0.03214224137010646 1107 4 0.07714962469119513 1107 5 0.15055951705442538 1107 6 0.21028323573529661 1107 7 0.1481895047296362 1107 8 0.15811196868394717 1107 9 0.20088403392665555 1107 10-11 0.21498469461219116 1107 12-13 0.18409522635715092 1107 14-15 0.2243910407724954 1107 16-17 0.3174712009709424 1107 18-19 0.25153927437879275 1107 20-21 0.25137476766395395 1107 22-23 0.17857332204073373 1107 24-25 0.19839503015000304 1107 26-27 0.2941893253055383 1107 28-29 0.25729730886598645 1107 30-31 0.2946523629895097 1107 32-33 0.35430249146123316 1107 34-35 0.3607246861675506 1107 36-37 0.29480393828607276 1107 38-39 0.28222176391602716 1107 40-41 0.2790933928706707 1107 42-43 0.3133784858075046 1107 44-45 0.2843666247257417 1107 46-47 0.3480083265222049 1107 48-49 0.31686677362129245 1107 50-51 0.31570264560613737 1107 52-53 0.3628008724355283 1107 54-55 0.2987921658262067 1107 56-57 0.3307623460568223 1107 58-59 0.3408999765574805 1107 60-61 0.3542339953312421 1107 62-63 0.3564533716870315 1107 64-65 0.3657816815209003 1107 66-67 0.31321830736721523 1107 68-69 0.33762029600369203 1107 70-71 0.35589686165077694 1107 72-73 0.40431832455750794 1107 74-75 0.3572101999773878 1107 76-77 0.30757095363399145 1107 78-79 0.28576004900919116 1107 80-81 0.2613982173317311 1107 82-83 0.3201068205511248 1107 84-85 0.2761079867348144 1107 86-87 0.24317767908675592 1107 88-89 0.2604556872765471 1107 90-91 0.21324530774683126 1107 92-93 0.26094610025643306 1107 94-95 0.1956121015880541 1107 96-97 0.12693599581351833 1107 98-99 0.2010911100307382 1107 100 0.21105900444807446 1108 1 0.15057582078768306 1108 2 0.32181319399002817 1108 3 0.1998139304821578 1108 4 0.14445893205797944 1108 5 0.15325495903252317 1108 6 0.11351245605180083 1108 7 0.1747670218483819 1108 8 0.10652804298216267 1108 9 0.14536748893126372 1108 10-11 0.1869567933353551 1108 12-13 0.1520695884767278 1108 14-15 0.21524163065713964 1108 16-17 0.23998013740114033 1108 18-19 0.19756509058142058 1108 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-0.773343673920877 2215 24-25 -0.8104782548920255 2215 26-27 -0.8489549471333717 2215 28-29 -0.7908586842056309 2215 30-31 -0.7813734585868275 2215 32-33 -0.8715836377897332 2215 34-35 -0.9618549675087849 2215 36-37 -0.8774199196991859 2215 38-39 -0.9502444817576077 2215 40-41 -0.9239300796167029 2215 42-43 -0.893089117917043 2215 44-45 -0.984971912677409 2215 46-47 -0.9911442935463057 2215 48-49 -0.9764917826266881 2215 50-51 -0.9081981302486781 2215 52-53 -0.8910206927429556 2215 54-55 -0.9616094434062248 2215 56-57 -0.9001629419138553 2215 58-59 -0.8914451238623968 2215 60-61 -0.9409639775999565 2215 62-63 -0.9462461059968348 2215 64-65 -0.9871587943717302 2215 66-67 -0.859300980450044 2215 68-69 -0.7560192990374333 2215 70-71 -0.7554391416062316 2215 72-73 -0.837062053136961 2215 74-75 -0.7157141976511525 2215 76-77 -0.7202391189766502 2215 78-79 -0.7134793659785359 2215 80-81 -0.6771556755579979 2215 82-83 -0.6666887810015325 2215 84-85 -0.7176455683790408 2215 86-87 -0.6960593125823635 2215 88-89 -0.6637371584335234 2215 90-91 -0.6305719732028408 2215 92-93 -0.7143480010924605 2215 94-95 -0.7664447045565836 2215 96-97 -0.7149673389995854 2215 98-99 -0.670831332194723 2215 100 -0.8206767512665252 2216 1 -0.3970507728760104 2216 2 -0.1570745787685972 2216 3 -0.3828480019074405 2216 4 -0.28469845438313257 2216 5 -0.33940847232885574 2216 6 -0.40657550325862246 2216 7 -0.4591304726306049 2216 8 -0.5052774935540185 2216 9 -0.584792952094304 2216 10-11 -0.6311491758215553 2216 12-13 -0.5665857582535097 2216 14-15 -0.7740628179710782 2216 16-17 -0.8209281805964466 2216 18-19 -0.7650193762818347 2216 20-21 -0.733284962008689 2216 22-23 -0.6752496661570007 2216 24-25 -0.6578285154015688 2216 26-27 -0.7498953002614215 2216 28-29 -0.7987047663791671 2216 30-31 -0.8482431674094215 2216 32-33 -0.8617541804101378 2216 34-35 -0.8081342634565871 2216 36-37 -0.8387669048380175 2216 38-39 -0.8613578667484632 2216 40-41 -0.8142968099574617 2216 42-43 -0.8741935052313607 2216 44-45 -0.8276452999705626 2216 46-47 -0.8041983037108764 2216 48-49 -0.8105964203410636 2216 50-51 -0.7877805868947831 2216 52-53 -0.7824280346305557 2216 54-55 -0.8142668080462911 2216 56-57 -0.8272413732188895 2216 58-59 -0.9494556878922538 2216 60-61 -0.8568156797547246 2216 62-63 -0.9368009069632066 2216 64-65 -0.8595058695365196 2216 66-67 -0.7400526922961319 2216 68-69 -0.7807837680048024 2216 70-71 -0.8312258479695842 2216 72-73 -0.858353143469401 2216 74-75 -0.8547136402773106 2216 76-77 -0.817499427318026 2216 78-79 -0.7254506048980929 2216 80-81 -0.6463053041148257 2216 82-83 -0.6301688402514074 2216 84-85 -0.6432762543424246 2216 86-87 -0.6334920083384006 2216 88-89 -0.6536060894908573 2216 90-91 -0.7491554466995467 2216 92-93 -0.6042692751122978 2216 94-95 -0.586454228614425 2216 96-97 -0.7133016619625536 2216 98-99 -0.6590725643795423 2216 100 -0.7134599653113618 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 3.0 12 7.0 13 59.0 14 242.0 15 765.0 16 1995.0 17 4026.0 18 7006.0 19 10897.0 20 15548.0 21 21877.0 22 29783.0 23 35117.0 24 37575.0 25 39721.0 26 43303.0 27 52972.0 28 65853.0 29 83116.0 30 103133.0 31 126870.0 32 158156.0 33 201293.0 34 272200.0 35 373736.0 36 556472.0 37 954759.0 38 943799.0 39 352406.0 40 974.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.505981460255285 20.961818809049532 13.750280989528271 24.781918741166916 2 14.871210431886029 20.312058385375703 40.11312900944352 24.703602173294755 3 18.01542332699763 26.137894860293 29.913857160310602 25.932824652398768 4 12.1578961192871 17.175145166682917 38.6410270450995 32.02593166893048 5 12.638254026891937 38.54675823515535 35.27308091170881 13.541906826243896 6 29.9378413695372 38.76575996781246 17.683921183248234 13.612477479402111 7 26.254260462110484 32.37372608956117 23.497789321984563 17.874224126343787 8 25.935472701118726 35.393545222784795 19.761090281783417 18.90989179431306 9 25.98015784001652 14.725644819016287 21.22475111623833 38.069446224728864 10-11 25.226863868771677 26.07847404701375 27.843626047697377 20.851036036517193 12-13 25.716475464120148 23.880746313030972 28.193429682326048 22.209348540522832 14-15 22.9923720933545 24.96917590388045 25.961021873312745 26.0774301294523 16-17 21.732802969963657 27.542910095263785 27.1205577450032 23.60372918976936 18-19 21.28506937768378 27.20648450796499 29.6182358093529 21.890210304998327 20-21 22.58525703371293 26.40672867771638 29.100879093808025 21.907135194762667 22-23 22.772671845776337 26.17088257943829 28.848829214780615 22.20761636000476 24-25 22.29263247096356 26.29915142743205 29.485270816865704 21.92294528473869 26-27 22.26498373151311 26.477655059119996 28.718936492037557 22.538424717329338 28-29 21.679691672541605 26.80955897014107 29.160569192534197 22.350180164783126 30-31 22.46021286860789 26.45163278785419 29.01054684996475 22.077607493573172 32-33 22.693852944946485 26.669951291418315 28.379068840037885 22.257126923597315 34-35 22.663586758173775 26.556844785074013 28.971112505674686 21.808455951077523 36-37 22.491812917031627 26.273070260704852 28.679936906720215 22.55517991554331 38-39 22.56106598090111 26.33869599507217 29.134309997365175 21.965928026661537 40-41 22.221576866535546 25.780468688454768 29.526380137643244 22.471574307366446 42-43 22.374995271083733 25.91614129334191 28.81843854522958 22.890424890344775 44-45 22.524403248663006 26.6527604200047 28.52598681165303 22.296849519679263 46-47 22.627314814814813 26.52753966473684 27.868505522442266 22.976639998006082 48-49 23.211133243621376 26.391783411269394 27.792162302239987 22.604921042869243 50-51 22.8018605752455 26.451043068640644 27.55990434531821 23.187192010795645 52-53 22.321662233758467 26.927925630398715 27.47143533650936 23.278976799333464 54-55 22.404767529102465 26.871104849433596 27.345585663854216 23.378541957609723 56-57 23.2003645135591 26.43426516469871 27.155670433455136 23.20969988828705 58-59 22.330557715391528 26.64553167644861 27.894081297759016 23.129829310400847 60-61 22.456563520222215 27.158176982479425 27.36795033090513 23.017309166393225 62-63 22.688690120133593 26.52018486473399 27.123857057403487 23.667267957728928 64-65 22.091504838813048 26.665456073262266 27.439679513199028 23.803359574725658 66-67 22.604371399374763 26.4171286504732 27.322069473819138 23.656430476332897 68-69 22.307434761946464 26.324647685080148 27.558257570961192 23.809659982012196 70-71 22.328498600073726 25.98310563877151 27.73199259084444 23.956403170310324 72-73 22.741965131349385 26.08896659830375 27.934042242588674 23.235026027758195 74-75 22.801866810395282 26.432853975851277 27.469215475868857 23.29606373788458 76-77 22.572895314221572 26.43644766823789 27.175409638696323 23.81524737884422 78-79 22.35372411299925 26.498336927486744 27.255918669951456 23.89202028956255 80-81 22.069330180936426 26.87073051101232 27.614803298622547 23.445136009428705 82-83 22.781126813878863 26.044904270680302 28.32912900322382 22.84483991221701 84-85 23.008626418356144 26.45843709649206 27.202271506291666 23.33066497886013 86-87 21.929614697095406 26.95608079236267 27.639532316588983 23.474772193952948 88-89 22.668896054602055 26.060704456023633 27.474572759374993 23.795826729999327 90-91 22.349521896444056 26.043390963324253 27.693051864321227 23.91403527591046 92-93 22.636317162643635 26.334940409542927 27.336853722681543 23.691888705131895 94-95 21.719848370611636 26.229819022126637 27.89485818459547 24.155474422666252 96-97 22.012222892703797 26.1910243424128 27.567070628415312 24.229682136468085 98-99 21.535952654120514 26.70973487780705 27.48944908941544 24.26486337865699 100 22.24209953082177 26.167783837238694 26.82960021292874 24.760516419010795 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 424.0 1 296.0 2 236.5 3 294.0 4 204.0 5 166.0 6 329.5 7 870.5 8 1486.5 9 1646.0 10 1312.0 11 931.0 12 911.5 13 1016.0 14 1166.0 15 1480.5 16 1839.0 17 2311.0 18 2989.5 19 3814.0 20 4825.5 21 5911.5 22 7154.0 23 8679.0 24 10295.0 25 12288.5 26 15541.5 27 19259.0 28 23643.5 29 32071.0 30 43958.5 31 54580.0 32 68946.0 33 82579.5 34 92439.5 35 104269.5 36 116099.5 37 135782.5 38 144433.0 39 137291.5 40 130758.5 41 122755.5 42 121428.0 43 128668.5 44 138716.5 45 159127.0 46 178194.0 47 190218.5 48 200098.0 49 197859.5 50 189760.0 51 188976.0 52 186782.5 53 179854.5 54 172759.0 55 153182.0 56 135445.5 57 115984.5 58 95410.0 59 89485.0 60 75305.5 61 54727.5 62 41383.0 63 33040.5 64 24206.0 65 14086.5 66 8973.5 67 6198.0 68 4251.5 69 3065.5 70 2308.0 71 1792.5 72 1391.0 73 1150.0 74 890.0 75 657.5 76 471.0 77 324.0 78 190.5 79 101.0 80 67.0 81 28.0 82 13.0 83 7.5 84 4.0 85 3.5 86 2.5 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0136636829099817 2 0.004673246597876477 3 0.0018025379734666412 4 0.02763891559315516 5 0.0027594408482699197 6 0.0 7 4.450711045596645E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.009480014527120852 16-17 0.0031377512871456343 18-19 0.0 20-21 5.563388806995806E-5 22-23 7.677476553654213E-4 24-25 0.0 26-27 1.5577488659588256E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 5.340853254715973E-4 36-37 0.0 38-39 0.0 40-41 4.00563994103698E-4 42-43 7.566208777514296E-4 44-45 0.0 46-47 0.0 48-49 5.118317702436141E-4 50-51 0.0 52-53 0.0 54-55 3.4493010603373997E-4 56-57 8.90142209119329E-5 58-59 2.2253555227983224E-4 60-61 1.001409985259245E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014131007569769347 70-71 7.677476553654213E-4 72-73 0.0 74-75 2.2253555227983224E-4 76-77 0.002915215734865802 78-79 5.229585478576058E-4 80-81 0.0033825403946534495 82-83 0.0018359183063086158 84-85 0.005741417248819672 86-87 0.008189308323897826 88-89 0.007855504995478078 90-91 0.006364516795203202 92-93 0.009146211198701104 94-95 0.0016912701973267248 96-97 0.004684373375490468 98-99 0.0021029609690444147 100 0.08676661183390659 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4493664.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.35614312574736 #Duplication Level Percentage of deduplicated Percentage of total 1 68.12093115576927 21.36009667179484 2 15.902472573339901 9.972804121258358 3 6.377159479047298 5.998893760821707 4 3.0275223615013283 3.7972569793454514 5 1.7059595336996338 2.674615565270947 6 1.0639102347107803 2.0016072955523216 7 0.6953362968322181 1.5262145110798726 8 0.4801877522618067 1.20454687097217 9 0.3613449748371354 1.0197346273886516 >10 1.801461039448652 10.507005073677918 >50 0.19961610077916062 4.438963362925208 >100 0.20672578512218534 13.9886040317968 >500 0.0364451971221946 8.011987204987024 >1k 0.02007333122130451 11.204251671294383 >5k 6.406382304671652E-4 1.5327770905502949 >10k+ 2.135460768223884E-4 0.7606411612841073 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 13617 0.3030266615394476 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 10241 0.2278986590897762 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 10221 0.22745358798521653 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9533 0.21214314198836404 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 8885 0.19772283820063097 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8834 0.1965879068840038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8830 0.19649889266309187 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 8685 0.19327212715503428 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7388 0.16440926602434006 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5798 0.12902611321184673 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5439 0.12103708688500074 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5281 0.1175210251589794 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4992 0.11108974769809224 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 4938 0.10988805571578117 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 4840 0.10770720730343879 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.2253555227983224E-5 7 0.0 0.0 0.0 0.0 2.2253555227983224E-5 8 0.0 0.0 0.0 0.0 2.2253555227983224E-5 9 0.0 0.0 0.0 0.0 2.2253555227983224E-5 10-11 0.0 0.0 0.0 0.0 2.2253555227983224E-5 12-13 0.0 0.0 0.0 0.0 1.5577488659588256E-4 14-15 0.0 0.0 0.0 0.0 2.1140877466584062E-4 16-17 0.0 0.0 0.0 1.1126777613991612E-5 2.4478910750781545E-4 18-19 0.0 0.0 0.0 2.2253555227983224E-5 2.4478910750781545E-4 20-21 0.0 0.0 0.0 2.2253555227983224E-5 3.671836612617232E-4 22-23 0.0 0.0 0.0 8.90142209119329E-5 3.7831043887571477E-4 24-25 0.0 0.0 0.0 1.6690166420987417E-4 3.7831043887571477E-4 26-27 0.0 0.0 0.0 3.894372164897064E-4 4.339443269456728E-4 28-29 0.0 0.0 0.0 0.0011349313166271443 4.4507110455966443E-4 30-31 0.0 0.0 0.0 0.0049625428158402585 4.4507110455966443E-4 32-33 0.0 0.0 0.0 0.015065656889344642 4.4507110455966443E-4 34-35 0.0 0.0 0.0 0.031266245095316426 4.895782150156309E-4 36-37 0.0 0.0 0.0 0.06019586689169462 4.895782150156309E-4 38-39 0.0 0.0 0.0 0.10723988264365114 5.118317702436141E-4 40-41 0.0 0.0 0.0 0.16104897918491456 5.118317702436141E-4 42-43 0.0 0.0 0.0 0.21947568843598453 5.118317702436141E-4 44-45 0.0 0.0 0.0 0.2901529798400593 5.452121030855889E-4 46-47 0.0 0.0 0.0 0.38178199349128017 6.00845991155547E-4 48-49 0.0 0.0 0.0 0.48740849338090253 6.564798792255051E-4 50-51 0.0 0.0 0.0 0.603538671338133 7.121137672954632E-4 52-53 0.0 0.0 0.0 0.7208149073896046 7.343673225234463E-4 54-55 0.0 0.0 0.0 0.8535796178797525 7.566208777514295E-4 56-57 0.0 0.0 0.0 1.0155298660514003 7.677476553654212E-4 58-59 0.0 0.0 0.0 1.2077894564435614 7.788744329794128E-4 60-61 0.0 0.0 0.0 1.416427663483518 7.900012105934044E-4 62-63 0.0 0.0 0.0 1.6298281313422631 8.456350986633625E-4 64-65 0.0 0.0 0.0 1.8577156636544254 8.456350986633625E-4 66-67 0.0 0.0 0.0 2.1200405726818916 8.456350986633625E-4 68-69 0.0 0.0 0.0 2.4175483525247996 8.456350986633625E-4 70-71 0.0 0.0 0.0 2.7522306963760528 8.678886538913456E-4 72-73 0.0 0.0 0.0 3.10375898153489 9.346493195752953E-4 74-75 0.0 0.0 0.0 3.4523497974036332 0.0010236635404872283 76-77 0.0 1.1126777613991612E-5 0.0 3.8458371609448325 0.0010681706509431948 78-79 0.0 2.2253555227983224E-5 0.0 4.2796145862262955 0.001090424206171178 80-81 0.0 2.2253555227983224E-5 0.0 4.7575319383024635 0.0011238045390131526 82-83 0.0 2.2253555227983224E-5 0.0 5.255299461641992 0.0012239455375390772 84-85 0.0 2.2253555227983224E-5 0.0 5.746168382860846 0.0012461990927670604 86-87 2.2253555227983224E-5 2.2253555227983224E-5 0.0 6.25501817670391 0.001346340091292985 88 2.2253555227983224E-5 2.2253555227983224E-5 0.0 6.668656134504048 0.0014464810898189094 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8535 0.0 45.832672 1 GTATCAA 14405 0.0 37.01308 1 GTACTAG 3695 0.0 31.047798 1 GGGCACC 4150 0.0 30.264688 94 CAACGCA 17080 0.0 29.772213 5 TAGGCAT 4710 0.0 29.136251 5 ATCAACG 17455 0.0 29.085865 3 GTGGTAT 2915 0.0 28.22636 1 TATCAAC 18930 0.0 28.062428 2 CTGTGCG 4865 0.0 27.247395 9 AACGCAG 19200 0.0 27.243372 6 TAGTACT 5145 0.0 25.856483 4 TCAACGC 20100 0.0 25.561863 4 TGAACCG 580 0.0 25.119179 5 ACGCAGA 20495 0.0 24.71939 7 CTAGGCA 5510 0.0 24.655548 4 CGCAGAG 20760 0.0 24.381212 8 GTACTGT 5955 0.0 24.228333 6 TACTGTG 6915 0.0 23.991058 7 ACTGTGC 6170 0.0 23.917257 8 >>END_MODULE