FastQCFastQC Report
Mon 16 Jan 2017
ERR1146435_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146435_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712516
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC142940.38502190966988425No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT127850.34437562019934725No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA121470.32719050907794067No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN114330.3079582687320405No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC103270.2781671513334892No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC95540.25734569224752163No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA93910.2529551387791999No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC79360.21376338849448728No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT63120.17001946927636138No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT61040.16441679981985263No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC59410.16002624635153087No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG59330.15981075906474207No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC57410.15463906418181095No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT55270.14887477926021062No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT51180.13785799172313332No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA50560.13618796525052015No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG49690.13384454100669196No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC48310.1301273853095852No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA46990.12657184507757002No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC46880.12627555005823543No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC46060.12406680536865027No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT45370.12220822752009687No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG45160.1216425733922763No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA44200.11905672595081071No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT43490.11714427628056014No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA43060.11598603211407034No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA42180.11361567195939358No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT41720.11237662006035799No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG40120.10806687432458202No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC40070.10793219477033904No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT40070.10793219477033904No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT39890.10744734837506424No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC39340.10596587327839126No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA39290.10583119372414825No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG39260.10575038599160247No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG38230.10297598717419669No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC39750.037.64195394
GTACTAG40950.033.972541
TATTCTC22200.032.168375
GTATCAA83750.031.6454621
TACCCCG2900.030.7818015
TAGGCAT49200.030.7488735
CAACGCA78150.030.6605285
GGTATCA55550.030.0523971
GCATCGT43800.029.4771298
TAACGCC19600.029.2471394
ATCAACG82750.028.9612373
CTAACGC19200.028.8799023
CGTTAAC9650.028.8326551
TAGTACT57300.028.782744
CTAGGCA55600.028.4796564
TCTAACG20050.027.8906922
CTGTGCG58350.027.681989
AGGGCTA48550.027.289675
CGCCTAC21000.026.8280167
TACTGTG68700.026.1739127
Produced by FastQC (version 0.11.5)