##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146435_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3712516
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.453688011041567	31.0	30.0	34.0	27.0	34.0
2	30.508798345919587	31.0	30.0	34.0	26.0	34.0
3	30.744048241138895	31.0	31.0	34.0	27.0	34.0
4	34.21560499671921	37.0	35.0	37.0	30.0	37.0
5	34.4093870571871	37.0	35.0	37.0	30.0	37.0
6	34.61901874631651	37.0	35.0	37.0	31.0	37.0
7	34.459512093685255	37.0	35.0	37.0	30.0	37.0
8	34.43319301519509	37.0	35.0	37.0	30.0	37.0
9	35.816170219872454	39.0	35.0	39.0	30.0	39.0
10-11	35.72531579661879	39.0	35.0	39.0	30.0	39.0
12-13	35.70804718417375	39.0	35.0	39.0	30.0	39.0
14-15	36.72329385247094	40.0	36.0	41.0	29.5	41.0
16-17	36.55730184058466	40.0	36.0	41.0	28.0	41.0
18-19	36.508406024378075	40.0	36.0	41.0	27.5	41.0
20-21	36.85535415874302	40.0	36.0	41.0	30.0	41.0
22-23	36.57261086551546	40.0	36.0	41.0	28.5	41.0
24-25	36.69013601557542	40.0	36.0	41.0	29.5	41.0
26-27	36.76579885446958	40.0	36.0	41.0	30.0	41.0
28-29	36.620164195925355	40.0	35.5	41.0	29.5	41.0
30-31	36.57348722537492	40.0	35.0	41.0	29.5	41.0
32-33	36.6834790745683	40.0	35.0	41.0	30.0	41.0
34-35	36.73103361709417	40.0	35.0	41.0	30.0	41.0
36-37	36.6586620771466	40.0	35.0	41.0	30.0	41.0
38-39	36.48679466970648	39.0	35.0	41.0	29.0	41.0
40-41	36.43987918166548	39.0	35.0	41.0	29.0	41.0
42-43	36.27801469407809	39.0	35.0	41.0	29.0	41.0
44-45	36.07733192260989	39.0	35.0	41.0	28.0	41.0
46-47	35.936733336637474	38.5	34.5	40.5	28.0	41.0
48-49	35.80321835650001	38.0	34.0	40.0	28.0	41.0
50-51	35.57836087440431	38.0	34.0	40.0	27.0	41.0
52-53	35.38805193028124	38.0	34.0	40.0	27.0	41.0
54-55	35.188999050778506	37.5	33.5	40.0	27.0	41.0
56-57	35.03022990338628	37.0	33.0	40.0	27.0	41.0
58-59	34.764441284562814	36.5	33.0	40.0	26.0	41.0
60-61	34.508532084440844	36.0	33.0	39.5	26.0	41.0
62-63	34.239898360034005	35.5	33.0	39.0	26.0	41.0
64-65	33.94832601394849	35.0	32.5	39.0	26.0	41.0
66-67	33.66256118492149	35.0	32.0	38.5	26.0	40.5
68-69	33.373560006206034	35.0	32.0	38.0	25.0	40.0
70-71	33.31752267195616	35.0	32.0	37.0	25.5	40.0
72-73	33.115374182899146	35.0	32.0	37.0	25.0	40.0
74-75	32.86083103749587	35.0	32.0	36.0	25.0	39.0
76-77	32.641048146324486	35.0	32.0	36.0	25.0	39.0
78-79	32.433709511285606	35.0	31.5	36.0	25.0	39.0
80-81	32.225201992395455	35.0	31.0	35.0	24.0	37.5
82-83	31.999026132143268	35.0	31.0	35.0	24.0	37.0
84-85	31.82789286295332	35.0	31.0	35.0	24.0	37.0
86-87	31.674354669447887	34.5	31.0	35.0	24.0	36.0
88-89	31.68333914251144	35.0	31.0	35.0	24.0	36.0
90-91	31.607875494678005	35.0	31.0	35.0	24.0	36.0
92-93	31.470450901760422	35.0	31.0	35.0	23.5	36.0
94-95	31.457188063297234	35.0	31.0	35.0	24.0	35.0
96-97	31.37255368057673	35.0	31.0	35.0	23.0	35.0
98-99	31.295433743585214	35.0	31.0	35.0	23.0	35.0
100	30.801464289985553	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6825517830277157
1101	2	0.8351102967125712
1101	3	0.7999053467269626
1101	4	0.7891356844350952
1101	5	0.7454270279173443
1101	6	0.552419319489232
1101	7	0.6228761059326757
1101	8	0.6020918501091757
1101	9	0.7993851251612298
1101	10-11	0.8548650378354807
1101	12-13	0.7801600578013677
1101	14-15	0.9679051443732689
1101	16-17	1.0447277826232693
1101	18-19	1.2268147360277482
1101	20-21	0.8508855947251703
1101	22-23	1.1710614227506184
1101	24-25	1.0513049258038407
1101	26-27	0.8828986413222353
1101	28-29	1.0618218682220473
1101	30-31	1.052601304959019
1101	32-33	0.9567105014246806
1101	34-35	0.9290407923012651
1101	36-37	0.8861831408759286
1101	38-39	1.0119666054377774
1101	40-41	0.8519600956026139
1101	42-43	0.8726271922679558
1101	44-45	0.8529336039689497
1101	46-47	0.8733025016951288
1101	48-49	0.8800994205122521
1101	50-51	0.8947121983022228
1101	52-53	0.8706202506370175
1101	54-55	0.919168982756041
1101	56-57	0.8102889655554293
1101	58-59	0.7473801339382291
1101	60-61	0.6916382997141355
1101	62-63	0.6515107797283122
1101	64-65	0.6326282256724269
1101	66-67	0.7257341055156346
1101	68-69	0.6899703911228912
1101	70-71	0.6428420607317307
1101	72-73	0.6077153365252315
1101	74-75	0.5644498799659203
1101	76-77	0.6748758897250653
1101	78-79	0.6938669887950795
1101	80-81	0.6792456950440098
1101	82-83	0.6177486177542093
1101	84-85	0.6312279730846733
1101	86-87	0.6467903555845922
1101	88-89	0.6091440140067839
1101	90-91	0.632798139038151
1101	92-93	0.6447064772508995
1101	94-95	0.5572263802866928
1101	96-97	0.5492623391233487
1101	98-99	0.5552023881436732
1101	100	0.6842467457009924
1102	1	0.7225765770286436
1102	2	0.8437127370351618
1102	3	0.8287768828096453
1102	4	0.8896625569548533
1102	5	0.7991306318062286
1102	6	0.6286073092212803
1102	7	0.6637292755896667
1102	8	0.71663474440453
1102	9	0.8780919293627392
1102	10-11	0.8235390304107568
1102	12-13	0.7975524093910806
1102	14-15	1.0139689282881577
1102	16-17	1.18474920787407
1102	18-19	1.135838270116139
1102	20-21	0.9519017782007779
1102	22-23	1.1847057029497776
1102	24-25	1.0577885907344822
1102	26-27	0.853520276783037
1102	28-29	1.1123027573025794
1102	30-31	1.1378256300299299
1102	32-33	0.9628332047792867
1102	34-35	0.8911280361920291
1102	36-37	0.8033273840188357
1102	38-39	0.8727959580313751
1102	40-41	0.8750043677167767
1102	42-43	0.8873540203202168
1102	44-45	0.9038793805669201
1102	46-47	0.8530947171873322
1102	48-49	0.7109335904185343
1102	50-51	0.875422061328841
1102	52-53	0.9066667191762576
1102	54-55	0.9051192734791726
1102	56-57	0.7992822482676729
1102	58-59	0.746161657017538
1102	60-61	0.7594871169459623
1102	62-63	0.7794527092136292
1102	64-65	0.7658008134789043
1102	66-67	0.864722335102698
1102	68-69	0.8667378058766673
1102	70-71	0.7310325702888818
1102	72-73	0.7014275644144377
1102	74-75	0.7093386438367446
1102	76-77	0.8068522343616849
1102	78-79	0.8159932764998103
1102	80-81	0.7832344636310964
1102	82-83	0.7069165701605158
1102	84-85	0.6827725450175954
1102	86-87	0.6762140298129751
1102	88-89	0.7278306393950338
1102	90-91	0.6977067111597179
1102	92-93	0.787839616993395
1102	94-95	0.7461025989320476
1102	96-97	0.6907869006805178
1102	98-99	0.667600567671105
1102	100	0.7016234326026201
1103	1	0.6771969952899397
1103	2	0.8426697555859946
1103	3	0.7785925598999839
1103	4	0.809128155641929
1103	5	0.8132111577622254
1103	6	0.61641466506277
1103	7	0.6881793318201375
1103	8	0.6969885694580782
1103	9	0.8509990105931848
1103	10-11	0.9109365579900981
1103	12-13	0.7510931935219887
1103	14-15	0.9165209499130853
1103	16-17	1.2363720295193943
1103	18-19	1.271567048873976
1103	20-21	0.9464587705864744
1103	22-23	1.1139852988766208
1103	24-25	1.0182072113406875
1103	26-27	0.9351665272767136
1103	28-29	1.148096211843125
1103	30-31	1.1240292813555968
1103	32-33	0.9313753155929163
1103	34-35	0.8713757419829449
1103	36-37	0.87116334338549
1103	38-39	0.98166985315342
1103	40-41	0.7926548525394637
1103	42-43	0.849024498128685
1103	44-45	0.8808954162301177
1103	46-47	0.9792266815806414
1103	48-49	0.9098968892151191
1103	50-51	0.9710673327797892
1103	52-53	0.9523276374986693
1103	54-55	0.9542563462151676
1103	56-57	0.889871061998349
1103	58-59	0.8768613715964904
1103	60-61	0.8102378596733146
1103	62-63	0.7347169831834464
1103	64-65	0.7757113944492389
1103	66-67	0.8173801995980199
1103	68-69	0.8978884697401099
1103	70-71	0.7473045488457544
1103	72-73	0.784318839080143
1103	74-75	0.7902389424017713
1103	76-77	0.7415276823167147
1103	78-79	0.7219040165492316
1103	80-81	0.7437864434900874
1103	82-83	0.7487299396828035
1103	84-85	0.7538397906338581
1103	86-87	0.7797552899527389
1103	88-89	0.7341137104935562
1103	90-91	0.8247767507943635
1103	92-93	0.8292047090227896
1103	94-95	0.7822299660012035
1103	96-97	0.7455471887672154
1103	98-99	0.8376823214228217
1103	100	0.8712103674803089
1104	1	0.45185630549638134
1104	2	0.6679312389700023
1104	3	0.6236533276089702
1104	4	0.6587066486826316
1104	5	0.5995856582197732
1104	6	0.43933347890904173
1104	7	0.4439101764816584
1104	8	0.4321869682594368
1104	9	0.5369268144798767
1104	10-11	0.5703062019285738
1104	12-13	0.45088595279845123
1104	14-15	0.6369036013913316
1104	16-17	0.8220018494546864
1104	18-19	0.738513291698979
1104	20-21	0.6533506257235047
1104	22-23	0.9157511691045883
1104	24-25	0.7247558743654992
1104	26-27	0.6070699049670552
1104	28-29	0.805955756994102
1104	30-31	0.843576271333724
1104	32-33	0.6282427692596002
1104	34-35	0.5501042086690546
1104	36-37	0.5350134453445747
1104	38-39	0.6151985397107325
1104	40-41	0.48247756593731594
1104	42-43	0.4534922602997611
1104	44-45	0.5476166762385049
1104	46-47	0.5398051415740923
1104	48-49	0.5371520073186176
1104	50-51	0.5728698878313949
1104	52-53	0.5587195697855663
1104	54-55	0.475547155109588
1104	56-57	0.47699751812788804
1104	58-59	0.4460558912689976
1104	60-61	0.4921067362737048
1104	62-63	0.4559582390359509
1104	64-65	0.5083351688777498
1104	66-67	0.6044777274973896
1104	68-69	0.5117569854360013
1104	70-71	0.4489731852711003
1104	72-73	0.41966524319474274
1104	74-75	0.37262380186419364
1104	76-77	0.4422539074959104
1104	78-79	0.3833729334720175
1104	80-81	0.46239375634696245
1104	82-83	0.39882504562300625
1104	84-85	0.3285444932573647
1104	86-87	0.3465317733403097
1104	88-89	0.30001432291375707
1104	90-91	0.39467703131558096
1104	92-93	0.3233490158971648
1104	94-95	0.28624868382025426
1104	96-97	0.4188485364054948
1104	98-99	0.43058119874358525
1104	100	0.491536410685665
1105	1	0.36101638059395214
1105	2	0.5238380426293041
1105	3	0.5312127200362049
1105	4	0.6319412451901698
1105	5	0.49361447374336365
1105	6	0.22025960879688
1105	7	0.29233969797131465
1105	8	0.33846629388640537
1105	9	0.3661155490771648
1105	10-11	0.440718072588858
1105	12-13	0.383492958270196
1105	14-15	0.5586946295874355
1105	16-17	0.6534770167938788
1105	18-19	0.6594661110155471
1105	20-21	0.5350313563082736
1105	22-23	0.7825512429702641
1105	24-25	0.5897209301715947
1105	26-27	0.4340980025651433
1105	28-29	0.6625333015163264
1105	30-31	0.6663357020599037
1105	32-33	0.5072535982987887
1105	34-35	0.4031459311534036
1105	36-37	0.3323566835972187
1105	38-39	0.44969639989488996
1105	40-41	0.3887677365413893
1105	42-43	0.42077440238525554
1105	44-45	0.4373000412539483
1105	46-47	0.3149549430630785
1105	48-49	0.41004617939569243
1105	50-51	0.41306252937662435
1105	52-53	0.4314930989819885
1105	54-55	0.3334128252686597
1105	56-57	0.32757620251113906
1105	58-59	0.3353317907220372
1105	60-61	0.28501602660835346
1105	62-63	0.29598524003309024
1105	64-65	0.3026717121245852
1105	66-67	0.41993645145461755
1105	68-69	0.41617406072662533
1105	70-71	0.34976423347948327
1105	72-73	0.2832705126313044
1105	74-75	0.416429480485931
1105	76-77	0.3660050904072989
1105	78-79	0.3465077705702697
1105	80-81	0.3856839378559229
1105	82-83	0.3895457896195893
1105	84-85	0.2420981621730327
1105	86-87	0.31611155264682367
1105	88-89	0.33804615969217977
1105	90-91	0.42795301609043435
1105	92-93	0.34669417724585827
1105	94-95	0.3318216742909357
1105	96-97	0.4119663199270498
1105	98-99	0.3757156806538653
1105	100	0.42533920969636085
1106	1	0.40913054777441715
1106	2	0.4858736554911971
1106	3	0.43366662361800934
1106	4	0.6081849557744476
1106	5	0.487966930762596
1106	6	0.315604607483678
1106	7	0.32707457921512173
1106	8	0.29431946981770807
1106	9	0.5032366171806615
1106	10-11	0.42452152638509943
1106	12-13	0.3387131459346051
1106	14-15	0.581368017378125
1106	16-17	0.6221046042749023
1106	18-19	0.6050243127106114
1106	20-21	0.4912395156134437
1106	22-23	0.7499996600857983
1106	24-25	0.570041914006481
1106	26-27	0.46504645534027844
1106	28-29	0.6759787037529321
1106	30-31	0.7016297574225803
1106	32-33	0.5012975937217021
1106	34-35	0.5302725757849487
1106	36-37	0.48394511172950416
1106	38-39	0.5483996380540148
1106	40-41	0.47938896444369306
1106	42-43	0.4945911062166033
1106	44-45	0.46084477676562585
1106	46-47	0.4791317869137188
1106	48-49	0.5100439035101658
1106	50-51	0.5648197624937552
1106	52-53	0.45814627423174414
1106	54-55	0.41856176554576763
1106	56-57	0.39963664078096883
1106	58-59	0.481592486471186
1106	60-61	0.4261108881943514
1106	62-63	0.41686470061658554
1106	64-65	0.4430897107522398
1106	66-67	0.43904083695213814
1106	68-69	0.41594193865932994
1106	70-71	0.432658160662335
1106	72-73	0.3571948646307135
1106	74-75	0.46347833242948155
1106	76-77	0.38844285710882787
1106	78-79	0.36345408109208677
1106	80-81	0.48701807533547736
1106	82-83	0.43037873572784235
1106	84-85	0.40915996475711935
1106	86-87	0.3457841099218584
1106	88-89	0.42307531171670476
1106	90-91	0.3779404013559855
1106	92-93	0.492728684051734
1106	94-95	0.4507898778578223
1106	96-97	0.3833185500879601
1106	98-99	0.43523361645734226
1106	100	0.47727242231445643
1107	1	0.447578231146899
1107	2	0.6271540457984912
1107	3	0.5494341981944437
1107	4	0.6493616197876335
1107	5	0.5798871505926471
1107	6	0.32579368527913743
1107	7	0.243668160601608
1107	8	0.36396381156922075
1107	9	0.46316114016731547
1107	10-11	0.5246438409611613
1107	12-13	0.411875887508657
1107	14-15	0.5851646279552156
1107	16-17	0.7219033723196446
1107	18-19	0.7941273219755516
1107	20-21	0.5206422514159215
1107	22-23	0.8053442144430889
1107	24-25	0.5093745468335626
1107	26-27	0.4689915881305424
1107	28-29	0.673757557428921
1107	30-31	0.6500127363965973
1107	32-33	0.5745535220953428
1107	34-35	0.4627490159044285
1107	36-37	0.47290465381239244
1107	38-39	0.4809404930119143
1107	40-41	0.3747926405801181
1107	42-43	0.5143773814635466
1107	44-45	0.5126560852138056
1107	46-47	0.5200055171438862
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2215	4	-1.9227083072214413
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2215	24-25	-5.423133416918205
2215	26-27	-3.50241603538867
2215	28-29	-1.6080956765027423
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2216	3	-0.5064464593994522
2216	4	-0.4386252033685878
2216	5	-0.5862698343002464
2216	6	-0.5133060469801762
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2216	9	-0.595150133854311
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2216	12-13	-0.8104615520385821
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2216	36-37	-1.0869925730475885
2216	38-39	-1.1244475244678682
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2216	56-57	-1.0226843081058377
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2216	64-65	-1.0238531749159705
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2216	68-69	-1.0563514611310865
2216	70-71	-1.0972227578900586
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2216	76-77	-1.0481712542431119
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2216	80-81	-1.095333401597813
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2216	84-85	-1.089135132336832
2216	86-87	-1.0298094284358967
2216	88-89	-0.9706978719681061
2216	90-91	-1.0258369208853573
2216	92-93	-0.9870092764189735
2216	94-95	-0.9141420926489978
2216	96-97	-0.9324269687649895
2216	98-99	-0.8601717864529768
2216	100	-0.7819557931840997
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	15260.0
1	2571.0
2	394.0
3	399.0
4	335.0
5	220.0
6	178.0
7	267.0
8	526.0
9	917.0
10	1595.0
11	2716.0
12	4225.0
13	6088.0
14	7919.0
15	10005.0
16	11952.0
17	14608.0
18	16804.0
19	19217.0
20	22025.0
21	25455.0
22	29517.0
23	33823.0
24	38382.0
25	43215.0
26	48368.0
27	55867.0
28	66348.0
29	79534.0
30	94688.0
31	113144.0
32	140207.0
33	180052.0
34	249569.0
35	336073.0
36	497450.0
37	750273.0
38	589768.0
39	202382.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.211311890595844	21.373406764955778	12.67163435600915	23.743646988439227
2	15.290767614814285	19.755708989409168	41.11944300588933	23.83408038988722
3	18.39987419722238	27.07626008479237	28.86664905572132	25.65721666226393
4	12.012103275656678	16.93822631425975	40.2096450582621	30.84002535182147
5	13.045022661342182	38.642505389728285	35.064223593713095	13.248248355216438
6	29.56228750260187	38.74111946627068	18.316169331514754	13.38042369961269
7	26.684490907309076	31.747560672534803	24.08053827907281	17.487410141083302
8	26.095431524852813	35.135541960735054	19.999664295709994	18.769362218702142
9	26.101421484237715	14.9836644931119	21.74983523693119	37.165078785719196
10-11	25.666799226952826	25.93691619820349	27.51720000178733	20.879084573056357
12-13	25.9264590035104	23.788712076632464	27.44968983760852	22.835139082248617
14-15	23.2816387830038	24.859934031328688	24.90737753206329	26.951049653604226
16-17	22.217025732239073	27.410255497149954	26.37570414959283	23.997014621018135
18-19	21.68552838492041	28.105088534218236	27.932778065578717	22.276605015282634
20-21	23.46693356654577	27.27220659935406	26.70431319790927	22.5565466361909
22-23	24.04557019454172	26.80801825796275	26.509967464942726	22.6364440825528
24-25	23.39856520129087	26.998126786813177	27.20698971865245	22.396318293243503
26-27	23.198241400273798	27.155727016351573	26.219856642012896	23.426174941361737
28-29	22.28572148002623	27.758492595157737	26.848096899491363	23.107689025324675
30-31	23.126672375221265	27.24583450381266	26.72112582624559	22.90636729472049
32-33	23.641424060032705	27.578633305056037	25.634959724487278	23.144982910423977
34-35	23.555794343576363	27.365152318533593	26.715902253134676	22.363151084755366
36-37	23.25442441532686	26.972299742567902	26.402235727848673	23.37104011425657
38-39	23.42474045045911	26.877267292356983	27.039368116876172	22.658624140307733
40-41	22.939510271657976	26.111216360985374	27.720943597999227	23.22832976935743
42-43	22.919973875896424	26.33984544637998	26.965052714709	23.775127963014594
44-45	23.084132071531478	27.365736277850655	26.779653350285443	22.770478300332424
46-47	23.09062708597441	27.07625086689186	26.284215397353705	23.548906649780022
48-49	23.794406811190278	26.560809769789547	26.56648251238323	23.078300906636947
50-51	23.37989160415612	26.476969877676215	26.502155540696915	23.64098297747075
52-53	22.773970353860022	27.13356062789842	26.399224340710592	23.69324467753097
54-55	22.765904867009482	27.10478440877869	26.3030565645555	23.826254159656326
56-57	23.92499428978846	26.424124049488412	26.077151693681916	23.573729967041217
58-59	22.814180088872664	26.779707742223234	27.010054163634784	23.396058005269317
60-61	22.775594535734424	27.539584378412375	26.288982717523684	23.395838368329517
62-63	23.24936608588956	26.728870511205074	25.883177844029987	24.138585558875388
64-65	22.34335409808852	26.983557222320538	26.388737502252745	24.284351177338195
66-67	23.200206238497927	26.52071104621627	26.276263183720154	24.00281953156565
68-69	22.604375986758445	26.658343294024156	26.55505275848162	24.182227960735784
70-71	22.627699372098604	26.23905315957405	26.758562624132797	24.37468484419455
72-73	23.227015107167933	26.514058068451796	27.075636044065565	23.18329078031471
74-75	23.40660653081033	26.88001282590139	26.663616362557036	23.04976428073125
76-77	23.05465656329339	26.826470664691048	26.245253212543435	23.87361955947213
78-79	22.921776434524862	26.74771328436263	26.382423852213527	23.948086428898982
80-81	22.52265499218149	27.155986605970828	27.032585629567706	23.288772772279977
82-83	23.439810412551463	26.08242309721691	28.043057678458204	22.434708811773422
84-85	23.69260988649771	26.795375087288882	26.469266391521906	23.042748634691502
86-87	22.30280896518536	27.500250937501146	27.00405343861797	23.192886658695524
88-89	23.395164551173124	26.121752247222457	26.785170258858727	23.69791294274569
90-91	23.028993348215543	26.165056363888166	27.07155213160911	23.73439815628718
92-93	23.33018948345448	26.48201019740345	26.934386863482747	23.253413455659324
94-95	22.1311436341201	26.469156320572118	27.60468352029093	23.79501652501686
96-97	22.50984560634923	26.40937432722314	27.109477586768726	23.971302479658902
98-99	21.900913942307756	27.238814150358003	26.96905281513267	23.891219092201567
100	22.710200560331494	26.500008714331152	26.085342712858434	24.70444801247892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13000.0
1	7627.5
2	2097.0
3	1547.5
4	886.0
5	527.0
6	418.5
7	393.5
8	395.5
9	379.0
10	341.0
11	346.5
12	354.0
13	344.0
14	353.5
15	480.0
16	577.0
17	640.0
18	808.5
19	1003.0
20	1244.0
21	1589.0
22	2081.5
23	2848.0
24	3753.5
25	4963.0
26	6942.0
27	8986.5
28	12429.0
29	19870.5
30	30103.5
31	40289.5
32	53766.5
33	65688.5
34	74129.5
35	83899.5
36	93398.0
37	112142.5
38	119875.0
39	109612.0
40	100537.5
41	91885.0
42	89766.0
43	96514.0
44	106723.5
45	128660.5
46	151196.5
47	165749.0
48	173988.5
49	170433.5
50	163434.0
51	167735.5
52	170249.5
53	164236.0
54	156842.5
55	136939.0
56	120467.0
57	100809.0
58	80683.5
59	75876.5
60	62661.5
61	44286.5
62	33986.5
63	28415.5
64	20894.0
65	11012.0
66	6535.5
67	4589.0
68	3249.0
69	2415.5
70	1902.0
71	1453.0
72	1136.0
73	923.0
74	698.0
75	544.0
76	423.0
77	288.0
78	183.0
79	126.5
80	88.5
81	62.0
82	49.0
83	30.5
84	26.5
85	28.0
86	29.0
87	24.5
88	16.0
89	11.5
90	11.0
91	11.5
92	9.5
93	7.5
94	5.0
95	4.0
96	4.5
97	3.5
98	3.0
99	2.5
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9938543025807834
2	0.44923712113294595
3	0.48064439318241325
4	0.4664761040760497
5	0.4345570497204591
6	0.4864356140148621
7	0.5028934555433566
8	0.50609882893434
9	0.5207789003468268
10-11	0.5348529137652202
12-13	0.532603765209362
14-15	0.5307047834945358
16-17	0.5347451701218258
18-19	0.532334406100876
20-21	0.5318630276610256
22-23	0.49419315633925887
24-25	0.5296542829714403
26-27	0.5089675034397158
28-29	0.5150684872469237
30-31	0.5179371617522995
32-33	0.5206442207925838
34-35	0.5331694193371826
36-37	0.5514993066696547
38-39	0.5403613075337588
40-41	0.5453040471744769
42-43	0.5425027124462224
44-45	0.5534521602061782
46-47	0.5507451011658939
48-49	0.5230145809472606
50-51	0.5370751264102297
52-53	0.5294926675063488
54-55	0.5401592882023942
56-57	0.5271492432625207
58-59	0.5337889452867004
60-61	0.5283074874290105
62-63	0.522273843398924
64-65	0.5333445027576986
66-67	0.5307451873608087
68-69	0.5522669801288399
70-71	0.535970754065437
72-73	0.5404286473108803
74-75	0.538394986041811
76-77	0.5250347742609056
78-79	0.5561996231127354
80-81	0.5300044498124722
82-83	0.5294387956846516
84-85	0.5396744418071195
86-87	0.8436461957335671
88-89	1.1084261993753024
90-91	1.098837015113201
92-93	1.0989043548903223
94-95	1.103335312224917
96-97	1.0963050394934326
98-99	1.0926956274397202
100	1.0883185419268226
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3712516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.913913492561843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6125543249601	21.573409412109672
2	12.432709379847294	7.189565669741336
3	4.8693804911705305	4.22378538852219
4	2.4142724174065595	2.7922425529748565
5	1.4067127788740308	2.03367857986225
6	0.8608750271676414	1.4934759638059207
7	0.5693830399703975	1.152416437328517
8	0.3922216785593501	0.9072530947017956
9	0.2888443772323427	0.751645920249797
>10	1.6154747841842114	8.790308415520263
>50	0.2082963832017543	4.264029050355091
>100	0.24842725390352058	15.89637034916001
>500	0.050261944911859015	10.216080340926874
>1k	0.029094112824674646	15.113598647420053
>5k	0.0010257539777930162	1.957815587660424
>10k+	4.662518080877347E-4	1.644324589661061
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	14294	0.38502190966988425	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12785	0.34437562019934725	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12147	0.32719050907794067	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	11433	0.3079582687320405	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10327	0.2781671513334892	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9554	0.25734569224752163	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9391	0.2529551387791999	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	7936	0.21376338849448728	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6312	0.17001946927636138	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6104	0.16441679981985263	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5941	0.16002624635153087	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5933	0.15981075906474207	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5741	0.15463906418181095	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5527	0.14887477926021062	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5118	0.13785799172313332	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5056	0.13618796525052015	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4969	0.13384454100669196	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4831	0.1301273853095852	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4699	0.12657184507757002	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4688	0.12627555005823543	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4606	0.12406680536865027	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4537	0.12220822752009687	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4516	0.1216425733922763	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4420	0.11905672595081071	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4349	0.11714427628056014	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4306	0.11598603211407034	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4218	0.11361567195939358	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4172	0.11237662006035799	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4012	0.10806687432458202	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4007	0.10793219477033904	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4007	0.10793219477033904	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	3989	0.10744734837506424	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	3934	0.10596587327839126	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3929	0.10583119372414825	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	3926	0.10575038599160247	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	3823	0.10297598717419669	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.693591084859971E-5	0.0
16-17	0.0	0.0	0.0	2.693591084859971E-5	0.0
18-19	0.0	0.0	0.0	2.693591084859971E-5	0.0
20-21	0.0	0.0	0.0	6.733977712149927E-5	0.0
22-23	0.0	0.0	0.0	1.0774364339439884E-4	0.0
24-25	0.0	0.0	0.0	3.0976297475889664E-4	0.0
26-27	0.0	0.0	0.0	5.52186172396294E-4	0.0
28-29	0.0	0.0	0.0	0.0015218789629458836	0.0
30-31	0.0	0.0	0.0	0.00561613741193304	0.0
32-33	0.0	0.0	0.0	0.015286129406580336	0.0
34-35	0.0	0.0	0.0	0.030868553832495267	0.0
36-37	0.0	0.0	0.0	0.05847786245230997	0.0
38-39	0.0	0.0	0.0	0.10463254569138558	0.0
40-41	0.0	0.0	0.0	0.15445051280587074	0.0
42-43	0.0	0.0	0.0	0.21047720737095812	0.0
44-45	0.0	0.0	0.0	0.27797859995754903	0.0
46-47	0.0	0.0	0.0	0.36671895824826073	0.0
48-49	0.0	0.0	0.0	0.46829427805833024	0.0
50-51	0.0	0.0	0.0	0.5794587821305013	0.0
52-53	0.0	0.0	0.0	0.6885761569781788	0.0
54-55	0.0	0.0	0.0	0.8126025584805561	0.0
56-57	0.0	0.0	0.0	0.9653830448138137	0.0
58-59	0.0	0.0	0.0	1.1513755092233946	0.0
60-61	0.0	0.0	0.0	1.3486406523231147	0.0
62-63	0.0	0.0	0.0	1.557838403928764	0.0
64-65	0.0	0.0	0.0	1.778537789466766	0.0
66-67	0.0	0.0	0.0	2.030698318875932	0.0
68-69	0.0	0.0	0.0	2.3147913705961134	0.0
70-71	0.0	0.0	0.0	2.6367024411477287	0.0
72-73	0.0	0.0	0.0	2.9816706513857447	0.0
74-75	0.0	1.3467955424299854E-5	0.0	3.3099924687193267	0.0
76-77	0.0	2.693591084859971E-5	0.0	3.6882534647662126	0.0
78-79	0.0	2.693591084859971E-5	0.0	4.104036184625198	0.0
80-81	0.0	2.693591084859971E-5	0.0	4.560748021018631	0.0
82-83	0.0	2.693591084859971E-5	0.0	5.0369614568664485	0.0
84-85	0.0	2.693591084859971E-5	0.0	5.505336542657325	0.0
86-87	0.0	2.693591084859971E-5	0.0	6.0021155464380485	0.0
88	0.0	2.693591084859971E-5	0.0	6.401884867297541	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3975	0.0	37.641953	94
GTACTAG	4095	0.0	33.97254	1
TATTCTC	2220	0.0	32.16837	5
GTATCAA	8375	0.0	31.645462	1
TACCCCG	290	0.0	30.781801	5
TAGGCAT	4920	0.0	30.748873	5
CAACGCA	7815	0.0	30.660528	5
GGTATCA	5555	0.0	30.052397	1
GCATCGT	4380	0.0	29.477129	8
TAACGCC	1960	0.0	29.247139	4
ATCAACG	8275	0.0	28.961237	3
CTAACGC	1920	0.0	28.879902	3
CGTTAAC	965	0.0	28.832655	1
TAGTACT	5730	0.0	28.78274	4
CTAGGCA	5560	0.0	28.479656	4
TCTAACG	2005	0.0	27.890692	2
CTGTGCG	5835	0.0	27.68198	9
AGGGCTA	4855	0.0	27.28967	5
CGCCTAC	2100	0.0	26.828016	7
TACTGTG	6870	0.0	26.173912	7
>>END_MODULE