##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3712516
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.819569262462437	31.0	31.0	34.0	30.0	34.0
2	31.81431298881944	31.0	31.0	34.0	30.0	34.0
3	31.976579225517142	33.0	31.0	34.0	30.0	34.0
4	35.73185543173417	37.0	35.0	37.0	35.0	37.0
5	35.632277409713524	37.0	35.0	37.0	33.0	37.0
6	35.66474784216418	37.0	35.0	37.0	33.0	37.0
7	35.59172620400828	37.0	35.0	37.0	33.0	37.0
8	35.577163842526204	37.0	35.0	37.0	33.0	37.0
9	37.174829145517485	39.0	37.0	39.0	33.0	39.0
10-11	37.11153042842105	39.0	37.0	39.0	33.0	39.0
12-13	37.04028278935363	39.0	37.0	39.0	33.0	39.0
14-15	38.22842164181918	40.0	37.0	41.0	33.0	41.0
16-17	38.23790362115611	40.0	37.0	41.0	33.0	41.0
18-19	38.29871346009014	40.0	38.0	41.0	33.0	41.0
20-21	38.31546557644465	40.0	38.0	41.0	33.0	41.0
22-23	38.28172767470901	40.0	38.0	41.0	33.0	41.0
24-25	38.202656096296955	40.0	37.0	41.0	33.0	41.0
26-27	38.05244152483114	40.0	37.0	41.0	33.0	41.0
28-29	37.985451375832454	40.0	37.0	41.0	33.0	41.0
30-31	37.851026635306084	40.0	37.0	41.0	33.0	41.0
32-33	37.71690438505854	40.0	36.0	41.0	32.5	41.0
34-35	37.61380961051751	40.0	36.0	41.0	32.0	41.0
36-37	37.52789765754545	40.0	36.0	41.0	32.0	41.0
38-39	37.40546990235194	40.0	36.0	41.0	31.5	41.0
40-41	37.270226175456216	39.5	36.0	41.0	31.0	41.0
42-43	37.1379681595985	39.0	35.0	41.0	31.0	41.0
44-45	36.92043926544693	39.0	35.0	41.0	31.0	41.0
46-47	36.654609973398095	39.0	35.0	41.0	30.0	41.0
48-49	36.37064325109979	39.0	35.0	41.0	29.5	41.0
50-51	36.15879877689416	39.0	35.0	41.0	29.0	41.0
52-53	35.957379712302924	38.0	35.0	40.0	29.0	41.0
54-55	35.750048349959975	38.0	34.5	40.0	28.0	41.0
56-57	35.48241771887313	37.5	34.0	40.0	28.0	41.0
58-59	35.18883959018628	37.0	34.0	40.0	28.0	41.0
60-61	34.923956691365106	36.5	34.0	40.0	27.0	41.0
62-63	34.64952541618676	36.0	34.0	39.0	27.0	41.0
64-65	34.36859571783664	35.5	33.0	39.0	26.0	41.0
66-67	34.14733067817082	35.0	33.0	39.0	26.0	41.0
68-69	33.9634225145427	35.0	33.0	38.0	26.0	41.0
70-71	33.76363724762398	35.0	33.0	37.5	26.0	40.0
72-73	33.572421371382646	35.0	33.0	37.0	26.0	40.0
74-75	33.39744003796886	35.0	33.0	36.5	26.0	39.0
76-77	33.246736310362024	35.0	33.0	36.0	26.0	39.0
78-79	33.141284508942185	35.0	33.0	36.0	26.0	39.0
80-81	33.00745559076378	35.0	33.0	36.0	26.0	38.0
82-83	32.82129922133669	35.0	33.0	35.0	26.0	37.0
84-85	32.68358951180278	35.0	33.0	35.0	26.0	37.0
86-87	32.55058766076698	35.0	33.0	35.0	26.0	36.5
88-89	32.44484764510106	35.0	33.0	35.0	26.0	36.0
90-91	32.35690876483765	35.0	33.0	35.0	26.0	36.0
92-93	32.27887394963416	35.0	33.0	35.0	26.0	36.0
94-95	32.23094445922926	35.0	33.0	35.0	25.0	35.0
96-97	32.176816207660785	35.0	33.0	35.0	25.0	35.0
98-99	32.14000559189509	35.0	33.0	35.0	25.0	35.0
100	31.63981919539202	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.45419321616807196
1101	2	0.6325733448921014
1101	3	0.48280343715246943
1101	4	0.3517958793184448
1101	5	0.3825973522097854
1101	6	0.3889493776149848
1101	7	0.37297480799909266
1101	8	0.3713509636957397
1101	9	0.38248430899883346
1101	10-11	0.4828195891769269
1101	12-13	0.49738695335468464
1101	14-15	0.6699982798886808
1101	16-17	0.6319827009516459
1101	18-19	0.6157990922573973
1101	20-21	0.6291667878164162
1101	22-23	0.6187155519184699
1101	24-25	0.6745247737320028
1101	26-27	0.6843179748091117
1101	28-29	0.7213416221682039
1101	30-31	0.7573289070896649
1101	32-33	0.7397739483380263
1101	34-35	0.7849499182966682
1101	36-37	0.7549617164670934
1101	38-39	0.730367428171931
1101	40-41	0.7823885767326786
1101	42-43	0.7574438157614551
1101	44-45	0.6858210217142897
1101	46-47	0.692413231712159
1101	48-49	0.7130111156300032
1101	50-51	0.6978969135289077
1101	52-53	0.7202399977003893
1101	54-55	0.6865329964011195
1101	56-57	0.7423807285929129
1101	58-59	0.7083008590686575
1101	60-61	0.7475649561413107
1101	62-63	0.6959269205141254
1101	64-65	0.7055269358197975
1101	66-67	0.6966353180430076
1101	68-69	0.6143184889875783
1101	70-71	0.626320182937917
1101	72-73	0.5863071200317336
1101	74-75	0.5633912567522472
1101	76-77	0.5269888870526316
1101	78-79	0.5120723556446478
1101	80-81	0.5524426922713772
1101	82-83	0.528633945799875
1101	84-85	0.5154189246585403
1101	86-87	0.5785930239703347
1101	88-89	0.522851489151094
1101	90-91	0.4680046045876267
1101	92-93	0.5789687383543622
1101	94-95	0.5317797817753274
1101	96-97	0.5525924430184261
1101	98-99	0.5401182991243374
1101	100	0.5759448699089837
1102	1	0.4096421694974097
1102	2	0.510100075924349
1102	3	0.4366478547966324
1102	4	0.3224688882186797
1102	5	0.304798763042335
1102	6	0.3437744384209225
1102	7	0.3216185491982344
1102	8	0.34894506973051875
1102	9	0.4554935461116756
1102	10-11	0.43737683434782326
1102	12-13	0.43196447840134766
1102	14-15	0.5787922336559532
1102	16-17	0.5731144662440855
1102	18-19	0.583893659810947
1102	20-21	0.5575338535676835
1102	22-23	0.5216345739029364
1102	24-25	0.5855700632087064
1102	26-27	0.5955780221063591
1102	28-29	0.6487960935102492
1102	30-31	0.621569624498683
1102	32-33	0.6622010654290449
1102	34-35	0.6216078149838395
1102	36-37	0.6164855368992335
1102	38-39	0.6241573057515524
1102	40-41	0.5811968813754902
1102	42-43	0.6438662368147945
1102	44-45	0.6070727818862309
1102	46-47	0.583346746059668
1102	48-49	0.5477848703715154
1102	50-51	0.5534460871745068
1102	52-53	0.5082932306898869
1102	54-55	0.5927247591333114
1102	56-57	0.6276144861641839
1102	58-59	0.5694766876127488
1102	60-61	0.6021633000637436
1102	62-63	0.5181098032209874
1102	64-65	0.5776453069617418
1102	66-67	0.5753641875578381
1102	68-69	0.49961204187358277
1102	70-71	0.553678308026349
1102	72-73	0.5340475308845001
1102	74-75	0.5019629403861856
1102	76-77	0.5006946132540904
1102	78-79	0.5306043554020974
1102	80-81	0.5081053927962245
1102	82-83	0.5491944384459018
1102	84-85	0.5288745548506242
1102	86-87	0.5102169973437327
1102	88-89	0.49898692638117836
1102	90-91	0.46773611520256253
1102	92-93	0.5314132718835367
1102	94-95	0.5334740946513676
1102	96-97	0.48165616629580654
1102	98-99	0.5099799445566546
1102	100	0.5656280726471046
1103	1	0.3083100510614969
1103	2	-0.16208509950179817
1103	3	0.1982731271197551
1103	4	0.19198939682952698
1103	5	0.2302322535507173
1103	6	0.26922454317357847
1103	7	0.30675426699807673
1103	8	0.29588181460390217
1103	9	0.35534189890609724
1103	10-11	0.3593933798011406
1103	12-13	0.38597904488626966
1103	14-15	0.5136128950195697
1103	16-17	0.48663866069892237
1103	18-19	0.49378579927724076
1103	20-21	0.457406897401647
1103	22-23	0.4570020005253994
1103	24-25	0.4958664877373451
1103	26-27	0.48883164084845987
1103	28-29	0.4828203152971966
1103	30-31	0.5438225467323505
1103	32-33	0.6040504887827183
1103	34-35	0.589649087714804
1103	36-37	0.6008123928270237
1103	38-39	0.5959994169951273
1103	40-41	0.5914991408512904
1103	42-43	0.5700423625450171
1103	44-45	0.6086079718383388
1103	46-47	0.6168106747234461
1103	48-49	0.6117363518646002
1103	50-51	0.5743941002521638
1103	52-53	0.6409601516374082
1103	54-55	0.6411192580679739
1103	56-57	0.6413804470479292
1103	58-59	0.5527078325952175
1103	60-61	0.5453440587420033
1103	62-63	0.5397613266642765
1103	64-65	0.5447996370145987
1103	66-67	0.5708515315574516
1103	68-69	0.49012950034257585
1103	70-71	0.4274055678553168
1103	72-73	0.3614457555864732
1103	74-75	0.4346985140935473
1103	76-77	0.4693811236547347
1103	78-79	0.4628382618500808
1103	80-81	0.4729407612976999
1103	82-83	0.4040532450088037
1103	84-85	0.40219485782564846
1103	86-87	0.38192364568252657
1103	88-89	0.41845051354699336
1103	90-91	0.47761649264379713
1103	92-93	0.5452478701719912
1103	94-95	0.5730494241262676
1103	96-97	0.524262581912339
1103	98-99	0.5218427452944354
1103	100	0.619348434954329
1104	1	0.33989400807249126
1104	2	0.39221105472567075
1104	3	0.3515600564098236
1104	4	0.24775472712727264
1104	5	0.2834932443167446
1104	6	0.2585049310103855
1104	7	0.2852699296108696
1104	8	0.3372685009372489
1104	9	0.3634301536048099
1104	10-11	0.3802064895258539
1104	12-13	0.37251015126298626
1104	14-15	0.4620025430045587
1104	16-17	0.4289418197543853
1104	18-19	0.4593239974984371
1104	20-21	0.5160585125918757
1104	22-23	0.46900653251572777
1104	24-25	0.4083553224033878
1104	26-27	0.409868776446082
1104	28-29	0.4806391374409458
1104	30-31	0.4734323229168922
1104	32-33	0.47202390666945604
1104	34-35	0.4771158056879159
1104	36-37	0.5087147924139046
1104	38-39	0.45182134367826166
1104	40-41	0.5180706207427548
1104	42-43	0.4889043531924031
1104	44-45	0.5561752128852078
1104	46-47	0.5600968111172833
1104	48-49	0.5215657448484521
1104	50-51	0.5347549952287096
1104	52-53	0.491465334706092
1104	54-55	0.5186716528166428
1104	56-57	0.5074288329751226
1104	58-59	0.5089327395695946
1104	60-61	0.4507904712016213
1104	62-63	0.4751973446942799
1104	64-65	0.38507773674548673
1104	66-67	0.4685163868069395
1104	68-69	0.44673486674027885
1104	70-71	0.4013405608262417
1104	72-73	0.4108638446518995
1104	74-75	0.3636275228971897
1104	76-77	0.34504756659865876
1104	78-79	0.35746419671276186
1104	80-81	0.3500590542812958
1104	82-83	0.3747337080697051
1104	84-85	0.3408489111420323
1104	86-87	0.2889620320828499
1104	88-89	0.30938326976600194
1104	90-91	0.3319392867577662
1104	92-93	0.3388068781744167
1104	94-95	0.32085977642658037
1104	96-97	0.3801313453437274
1104	98-99	0.386530042241084
1104	100	0.4639537925552837
1105	1	0.17364188716046414
1105	2	-0.3354553926048318
1105	3	0.07770682885475821
1105	4	0.10285696530286259
1105	5	0.135806046530611
1105	6	0.21711211000175723
1105	7	0.26626431694575814
1105	8	0.23875063183024992
1105	9	0.36807156413728137
1105	10-11	0.33269530392060176
1105	12-13	0.35470560483287983
1105	14-15	0.40019165591778716
1105	16-17	0.3867164819812743
1105	18-19	0.39847831337392137
1105	20-21	0.412136549452093
1105	22-23	0.3900367046800426
1105	24-25	0.37235530107293613
1105	26-27	0.4364045480338419
1105	28-29	0.40452001070007526
1105	30-31	0.3719976343049396
1105	32-33	0.39051509593443967
1105	34-35	0.39067456754862917
1105	36-37	0.43801021522241257
1105	38-39	0.50673389661344
1105	40-41	0.5001146640521981
1105	42-43	0.4799931373650139
1105	44-45	0.46870555934806646
1105	46-47	0.4267286380453541
1105	48-49	0.4483353964562653
1105	50-51	0.5241372833079794
1105	52-53	0.4638120511406214
1105	54-55	0.4995770334471814
1105	56-57	0.5077911510775976
1105	58-59	0.440032922352394
1105	60-61	0.38237867795410097
1105	62-63	0.39748262822811853
1105	64-65	0.46270257771079315
1105	66-67	0.47921903107599917
1105	68-69	0.4269283734869873
1105	70-71	0.4208287450139778
1105	72-73	0.40017513108548286
1105	74-75	0.39357140343611263
1105	76-77	0.29127089020186503
1105	78-79	0.3192798112508868
1105	80-81	0.3441760736214263
1105	82-83	0.31642143456345906
1105	84-85	0.36755041315339554
1105	86-87	0.3337529858620343
1105	88-89	0.3187454508982057
1105	90-91	0.29558096176388204
1105	92-93	0.28389345644274044
1105	94-95	0.30998819617440887
1105	96-97	0.26509373004994075
1105	98-99	0.23074254531332627
1105	100	0.3134929768955672
1106	1	0.27289550624441006
1106	2	0.2866330256693388
1106	3	0.24442730946336155
1106	4	0.1507202374031067
1106	5	0.21170908017647605
1106	6	0.16792231307646688
1106	7	0.21960908974787685
1106	8	0.14234333010795552
1106	9	0.15330318477953853
1106	10-11	0.2213692074975384
1106	12-13	0.278788757657253
1106	14-15	0.35849585101983905
1106	16-17	0.28638936299189766
1106	18-19	0.33977549753151237
1106	20-21	0.2997948239024666
1106	22-23	0.2998397712106282
1106	24-25	0.2584942393808589
1106	26-27	0.32354420659259375
1106	28-29	0.3338502966997723
1106	30-31	0.32984799105913254
1106	32-33	0.36477394833802634
1106	34-35	0.30377365181506377
1106	36-37	0.31577018280991354
1106	38-39	0.40706763636069354
1106	40-41	0.31613250456057784
1106	42-43	0.3677838573992105
1106	44-45	0.36564406127016014
1106	46-47	0.27924529278086396
1106	48-49	0.26037405803111113
1106	50-51	0.3572480585670803
1106	52-53	0.4401393731341159
1106	54-55	0.3295760220777382
1106	56-57	0.259469555796251
1106	58-59	0.27986574456483737
1106	60-61	0.2951881344896776
1106	62-63	0.23929957839060734
1106	64-65	0.3451001488662939
1106	66-67	0.3421939578764608
1106	68-69	0.3421984334705712
1106	70-71	0.2917435000787947
1106	72-73	0.2709011519540141
1106	74-75	0.28233643371269324
1106	76-77	0.23457042764943736
1106	78-79	0.20395299409017298
1106	80-81	0.2187334625697801
1106	82-83	0.22814817203165916
1106	84-85	0.20643210994653316
1106	86-87	0.2080170350737376
1106	88-89	0.178906312190648
1106	90-91	0.22251536100677782
1106	92-93	0.24760163217809605
1106	94-95	0.2425708990549964
1106	96-97	0.2418707219913543
1106	98-99	0.26209956213613594
1106	100	0.24527172625874272
1107	1	0.03855125666132864
1107	2	-0.5335693326253796
1107	3	-0.08919824229574047
1107	4	0.039325632839052105
1107	5	0.082983939396307
1107	6	0.18041793286745644
1107	7	0.15474800985580828
1107	8	0.17627007338206369
1107	9	0.168137001858085
1107	10-11	0.21999011648047428
1107	12-13	0.20084072975740952
1107	14-15	0.27749977431292905
1107	16-17	0.32822060445847256
1107	18-19	0.3488270706277561
1107	20-21	0.27746293558823254
1107	22-23	0.2483435433435659
1107	24-25	0.21722052367777422
1107	26-27	0.30373995653836516
1107	28-29	0.18907267144543027
1107	30-31	0.2942660931746772
1107	32-33	0.3006279958474707
1107	34-35	0.301794521241149
1107	36-37	0.33276146754361946
1107	38-39	0.37879927653670364
1107	40-41	0.43173100552299815
1107	42-43	0.35020124444871215
1107	44-45	0.46699780351461584
1107	46-47	0.43095573372681173
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2216	76-77	-0.7962207819484135
2216	78-79	-0.6289173785735613
2216	80-81	-0.5899279305521148
2216	82-83	-0.6505927408195262
2216	84-85	-0.686430782692824
2216	86-87	-0.5768434361272696
2216	88-89	-0.6531835215060227
2216	90-91	-0.6834763028865893
2216	92-93	-0.7001213398729078
2216	94-95	-0.6415569485668655
2216	96-97	-0.7189031700666249
2216	98-99	-0.5914201360869811
2216	100	-0.6740701036497363
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	14.0
13	73.0
14	227.0
15	774.0
16	1826.0
17	3646.0
18	6370.0
19	10278.0
20	14592.0
21	20554.0
22	28075.0
23	33535.0
24	34661.0
25	35987.0
26	38426.0
27	46178.0
28	58209.0
29	71595.0
30	88657.0
31	109202.0
32	136488.0
33	173110.0
34	235332.0
35	325925.0
36	485703.0
37	821044.0
38	669627.0
39	261774.0
40	628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.988992513238756	21.794473573545826	13.94036506817449	24.27616884504093
2	14.855027763965085	20.192971274922787	40.26510317140066	24.686897789711466
3	17.841867967230197	26.738599552531728	29.85784020329476	25.561692276943315
4	12.194684285485652	17.3997153700701	38.898705262528274	31.506895081915975
5	12.585200580969374	38.75320007240568	35.0646317654056	13.596967581219346
6	29.441058301162876	39.14154174689079	17.672597235944572	13.744802716001765
7	25.76387961091406	32.45083631066539	24.045736345574515	17.73954773284604
8	26.302216609975552	34.81364120720288	19.646110616088926	19.23803156673264
9	26.066473518228605	14.348625029494821	21.679744949247358	37.90515650302921
10-11	25.65333051763279	25.991362730827287	27.22219917705405	21.133107574485873
12-13	26.096964969309223	23.856543648566095	27.76428438288212	22.28220699924256
14-15	23.335678316288767	24.641352973003332	25.691221103068667	26.331747607639226
16-17	21.659278134951872	27.122782514534073	27.12010230585491	24.097837044659144
18-19	21.167087226021383	26.93213982108091	29.926120183724457	21.97465276917325
20-21	22.719227456111952	26.189625143501143	29.10279934297891	21.988348057408
22-23	23.192803415826628	25.706363408753198	28.90131363546801	22.199519539952163
24-25	22.64945120775237	25.881423810698728	29.619050800050424	21.850074181498478
26-27	22.527043032084233	26.05949238102857	28.58228761196269	22.831176974924507
28-29	21.60564425850286	26.70756166438071	29.22829154136979	22.458502535746646
30-31	22.588010395106714	26.14997484185927	28.98802321660028	22.273991546433738
32-33	23.086809592201085	26.45956273319765	28.109266061075562	22.344361613525706
34-35	22.922505050505052	26.333912457912454	28.922666666666668	21.820915824915822
36-37	22.692993107639133	25.894353047906055	28.608738655941146	22.803915188513667
38-39	22.843605253149075	25.74813145586443	29.331738906983833	22.076524384002656
40-41	22.322548339341857	25.130033529930202	29.857099143866243	22.690318986861698
42-43	22.553965414350223	25.33573459455215	28.862528648198303	23.24777134289932
44-45	22.771241928654316	26.31899768243423	28.45500194477276	22.454758444138694
46-47	22.856467150579284	26.16759092755425	27.66607605192813	23.30986586993834
48-49	23.73438315657005	25.895811930403735	27.61730780730387	22.752497105722348
50-51	23.12353670664315	26.005234724914317	27.317983276031672	23.55324529241086
52-53	22.45920286942871	26.598121058602846	27.205094873665193	23.737581198303253
54-55	22.582489843441184	26.569827225705573	26.999725926110486	23.847957004742756
56-57	23.68643486847581	25.932812050927257	26.78456710296616	23.596185977630775
58-59	22.477566366222707	26.33758706383236	27.709396294578383	23.47545027536656
60-61	22.555163772468436	26.998514618196	26.975376655196005	23.47094495413956
62-63	23.064762549171505	26.18698478336524	26.544478192147857	24.20377447531539
64-65	22.17726469057642	26.35363726378553	27.03927740648121	24.42982063915684
66-67	22.869746554627643	25.979820693028664	26.948516854876853	24.20191589746684
68-69	22.423067759577414	25.842891992571527	27.382432430794417	24.351607817056642
70-71	22.401778430709445	25.466166678316544	27.524854299133477	24.607200591840535
72-73	23.02616338892546	25.623633675922203	27.74668176514256	23.603521170009774
74-75	23.15958333832771	26.126485636329182	27.241553790381897	23.472377234961215
76-77	22.870014707387636	26.09500002693661	26.691134085044254	24.3438511806315
78-79	22.605600113777903	26.102789066978122	27.025564471988655	24.266046347255323
80-81	22.204307823924527	26.61652490782263	27.53952421177845	23.639643056474398
82-83	23.151330759559563	25.470124493343004	28.53411395043297	22.844430796664465
84-85	23.507758043276844	26.122371026007134	26.871918107021546	23.497952823694472
86-87	22.05501358550531	26.79258563959627	27.395686984246794	23.756713790651627
88-89	22.93329659008366	25.564156367310197	27.227327172373926	24.275219870232217
90-91	22.498627201175335	25.5108515420265	27.605546578656693	24.384974678141475
92-93	22.936414593594534	25.97652230319239	27.081660921651935	24.00540218156114
94-95	21.69947666783256	25.771910569822897	27.864592768057733	24.664019994286807
96-97	22.049596959549415	25.674435656496858	27.466564592806513	24.80940279114721
98-99	21.437508535466193	26.45159491667365	27.313922796556778	24.796973751303383
100	22.31093150588914	25.61850125493008	26.46956221676457	25.601005022416206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	268.0
1	183.0
2	132.5
3	167.0
4	123.0
5	105.5
6	216.0
7	553.5
8	962.0
9	1123.0
10	918.5
11	648.5
12	623.0
13	714.5
14	897.0
15	1248.5
16	1589.0
17	2005.0
18	2743.5
19	3601.5
20	4472.0
21	5393.0
22	6352.0
23	7333.0
24	8318.0
25	9480.5
26	11011.5
27	12603.0
28	15672.5
29	22514.5
30	32063.5
31	40956.0
32	54141.5
33	65774.5
34	72118.0
35	81332.5
36	90780.0
37	109449.5
38	117278.5
39	106799.5
40	97837.5
41	89381.5
42	86666.0
43	91837.5
44	102304.0
45	125918.0
46	149167.5
47	164508.0
48	173324.0
49	169017.5
50	162421.0
51	166794.0
52	168955.0
53	163310.5
54	158884.0
55	139948.0
56	122177.5
57	102494.5
58	81909.0
59	78864.5
60	65474.0
61	45152.5
62	33964.5
63	27714.0
64	19972.0
65	10131.5
66	5892.0
67	4083.5
68	2905.0
69	2172.5
70	1659.0
71	1300.5
72	1015.0
73	836.0
74	638.5
75	464.5
76	357.0
77	246.0
78	138.5
79	69.5
80	31.0
81	21.0
82	17.5
83	5.0
84	1.0
85	0.0
86	1.0
87	1.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013387147691754057
2	0.004040386627289956
3	0.0019932574027963783
4	0.027743988174057703
5	0.002693591084859971
6	0.0
7	5.387182169719942E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009036998089705203
16-17	0.0031919054355590655
18-19	0.0
20-21	5.387182169719942E-5
22-23	8.754171025794906E-4
24-25	0.0
26-27	1.8855137594019796E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.3097457357759534E-4
36-37	0.0
38-39	0.0
40-41	3.232309301831965E-4
42-43	6.060579940934934E-4
44-45	0.0
46-47	0.0
48-49	4.8484639527479476E-4
50-51	0.0
52-53	0.0
54-55	3.636347964560961E-4
56-57	1.3467955424299854E-4
58-59	2.693591084859971E-4
60-61	6.733977712149927E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.001198648032762687
70-71	7.946093700336915E-4
72-73	0.0
74-75	2.0201933136449783E-4
76-77	0.002585847441465572
78-79	5.387182169719942E-4
80-81	0.002909078371648769
82-83	0.0018451098931290802
84-85	0.00558920150108444
86-87	0.007744074368972416
88-89	0.007259227973697622
90-91	0.005643073322781639
92-93	0.008404004184763109
94-95	0.0014949430520972838
96-97	0.00461950871053485
98-99	0.001750834205158981
100	0.08474037552969468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3712516.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.479216518108373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.44207368893967	15.160449768838946
2	15.578536767877349	7.003866020808541
3	6.237596661604961	4.206488577265436
4	3.204816587769024	2.8816706390914058
5	1.8059863362546908	2.0298578940707235
6	1.1249806843000703	1.51732106286426
7	0.772973028009145	1.2163079641492793
8	0.5484769726945681	0.9863466099518245
9	0.38140544106808605	0.7716325941858715
>10	2.1538801570603217	9.172357940614791
>50	0.29054718935106005	4.636241653659055
>100	0.34306835582512873	17.2076673113644
>500	0.06800602216538797	10.782954349025529
>1k	0.045018071010890635	17.801376706460292
>5k	0.001915662596208112	2.650448849432877
>10k+	7.18373473578042E-4	1.9750120582168949
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	15978	0.43038198353892615	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13265	0.35730485740667517	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	12360	0.3329278580886924	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10863	0.2926047995483386	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10538	0.2838506285225437	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10378	0.27954088278676775	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8722	0.23493501442148668	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8710	0.23461178349130346	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8025	0.21616068456001267	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6776	0.18251773191011164	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6284	0.16926526377260057	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6187	0.1666524804202864	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6034	0.16253128606045067	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5666	0.15261887086816595	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5400	0.14545391858243842	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5389	0.14515762356310383	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5381	0.14494213627631505	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5363	0.14445728988104026	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5195	0.13993205685847548	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5166	0.1391509154438661	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5118	0.13785799172313332	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5062	0.13634958071561173	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4957	0.13352131007650878	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4906	0.13214757862323018	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4845	0.13050448806146558	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4806	0.1294539875383702	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4644	0.12509036998089706	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4618	0.12439003629883345	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4586	0.12352808715167826	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4514	0.12158870157057909	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4481	0.1206998165125753	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4455	0.1199994828305117	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4319	0.11633619895510215	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4182	0.11264597916884399	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4122	0.111029824517928	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4060	0.10935979804531482	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4055	0.10922511849107183	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4009	0.10798606659203625	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	3962	0.10672007878215203	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	3732	0.10052481928697413	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	3714	0.10003997289169933	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	1.3467955424299854E-5	0.0
14-15	0.0	0.0	0.0	5.387182169719942E-5	0.0
16-17	0.0	0.0	0.0	5.387182169719942E-5	1.3467955424299854E-5
18-19	0.0	0.0	0.0	5.387182169719942E-5	2.693591084859971E-5
20-21	0.0	0.0	0.0	9.427568797009898E-5	2.693591084859971E-5
22-23	0.0	0.0	0.0	1.3467955424299854E-4	2.693591084859971E-5
24-25	0.0	0.0	0.0	3.366988856074964E-4	2.693591084859971E-5
26-27	0.0	0.0	0.0	6.060579940934934E-4	2.693591084859971E-5
28-29	0.0	0.0	0.0	0.0015488148737944833	2.693591084859971E-5
30-31	0.0	0.0	0.0	0.00562960536735734	2.693591084859971E-5
32-33	0.0	0.0	0.0	0.015447744871671932	2.693591084859971E-5
34-35	0.0	0.0	0.0	0.031084041119284065	2.693591084859971E-5
36-37	0.0	0.0	0.0	0.05878762542706887	2.693591084859971E-5
38-39	0.0	0.0	0.0	0.10494230866614446	2.693591084859971E-5
40-41	0.0	0.0	0.0	0.15546060946269322	2.693591084859971E-5
42-43	0.0	0.0	0.0	0.21252433659545172	2.693591084859971E-5
44-45	0.0	0.0	0.0	0.2812513131256539	2.693591084859971E-5
46-47	0.0	0.0	0.0	0.37114991558285537	2.693591084859971E-5
48-49	0.0	0.0	0.0	0.4738700116039904	2.693591084859971E-5
50-51	0.0	0.0	0.0	0.5861119521101054	2.693591084859971E-5
52-53	0.0	0.0	0.0	0.6967242700098801	2.693591084859971E-5
54-55	0.0	0.0	0.0	0.8221917427426575	2.693591084859971E-5
56-57	0.0	0.0	0.0	0.976588383726831	2.693591084859971E-5
58-59	0.0	0.0	0.0	1.1640623232330851	2.693591084859971E-5
60-61	0.0	0.0	0.0	1.3621490116136874	2.693591084859971E-5
62-63	0.0	0.0	0.0	1.5730841294690716	2.693591084859971E-5
64-65	0.0	0.0	0.0	1.795924919919537	2.693591084859971E-5
66-67	0.0	0.0	0.0	2.050415405617107	2.693591084859971E-5
68-69	0.0	0.0	0.0	2.3374310036643613	2.693591084859971E-5
70-71	0.0	0.0	0.0	2.663234313333599	2.693591084859971E-5
72-73	0.0	0.0	0.0	3.0121890383772083	2.693591084859971E-5
74-75	0.0	0.0	0.0	3.3451168964659006	2.693591084859971E-5
76-77	0.0	0.0	0.0	3.7296808956513585	2.693591084859971E-5
78-79	0.0	0.0	0.0	4.15334236943356	2.693591084859971E-5
80-81	0.0	0.0	0.0	4.61844474205633	2.693591084859971E-5
82-83	0.0	0.0	0.0	5.103978003057765	2.693591084859971E-5
84-85	0.0	0.0	0.0	5.58246752337229	2.693591084859971E-5
86-87	0.0	0.0	0.0	6.088499012529509	2.693591084859971E-5
88	0.0	0.0	0.0	6.49559490113982	2.693591084859971E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8525	0.0	43.848904	1
GGGCACC	4020	0.0	36.627663	94
GTATCAA	14010	0.0	36.01203	1
GTGGTAT	2235	0.0	34.292255	1
GTACTAG	4250	0.0	33.854664	1
TAGGCAT	5050	0.0	31.45707	5
TATTCTC	2325	0.0	30.322239	5
CAACGCA	16265	0.0	29.705164	5
ATCAACG	16550	0.0	29.286713	3
CTAGGCA	5795	0.0	28.230856	4
TACCCCG	320	0.0	27.905933	5
TCTAACG	1995	0.0	27.337217	2
TATCAAC	18490	0.0	27.232769	2
CTAACGC	1970	0.0	26.966349	3
GCATCGT	5040	0.0	26.948639	8
AACGCAG	18615	0.0	26.913101	6
TAGTACT	5845	0.0	26.782925	4
AGGGCTA	4990	0.0	26.749197	5
CTGTGCG	5890	0.0	26.334242	9
CTATTGA	5205	0.0	26.007221	9
>>END_MODULE