FastQCFastQC Report
Mon 16 Jan 2017
ERR1146434_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146434_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4992949
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC157660.3157652922150817No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN152760.30595145273865204No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT132630.2656345979099726No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA132280.26493360937594196No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC115510.23134624447395716No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA110550.22141223553455083No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC104460.2092150350424168No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC90780.18181639748373155No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT71440.14308177391757856No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG70200.14059827168272698No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC69570.13933649232147174No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT68160.13651250994151953No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC65100.13038386732970836No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT59910.1199892087822247No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT58480.11712516991461358No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG58140.11644420962441235No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC56500.11315957763638282No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA55930.11201796773810428No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC55180.11051584945089565No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT53790.10773192355860234No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA53440.10703093502457166No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG53330.10681062434244773No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC52860.10586929688246366No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG50200.1005417840238304No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG47450.036.3807951
GGGCACC50400.034.28431394
GGTATCA69250.032.2157821
GTATCAA105650.032.009361
GCATCGT50900.031.8240998
CAACGCA98400.031.0366555
TATTCGC5750.031.032019
ATCAACG104650.029.1868823
TAGGCAT63650.029.0840235
CTGTGCG65450.027.764869
CTAGGCA71100.027.096324
TAGTACT68000.027.0186654
TATCAAC127200.026.4149632
AACGCAG124750.026.1558256
TATTCTC29950.025.5709865
ACGCAGA120400.025.1139037
ACTGTGC76600.025.0084158
GTACTGT75300.024.9395856
CGCAGAG121150.024.7646548
CTAGTAC79350.024.339323
Produced by FastQC (version 0.11.5)